Your browser doesn't support javascript.
Mostrar: 20 | 50 | 100
Resultados 1 - 3 de 3
Filtrar
Filtros adicionais











Intervalo de ano
2.
J Nat Prod ; 77(9): 2130-3, 2014 Sep 26.
Artigo em Inglês | MEDLINE | ID: mdl-25153802

RESUMO

A new granaticin analogue and its hydrolysis product were isolated from Streptomyces sp. CPCC 200532. Their structures were determined to be 6-deoxy-13-hydroxy-8,11-dione-dihydrogranaticins B (1) and A (2), respectively, by detailed analysis of spectroscopic data. Compound 1 was regarded as an intermediate in granaticin biosynthesis, as it was bioconvertable to granaticin B. Compared to granaticin B, 1 showed similar cytotoxicity against cancer cell line HCT116, but decreased cytotoxicity against cancer cell lines A549, HeLa, and HepG2. Compound 2 displayed lower cytotoxicity than 1 against all four cancer cell lines tested.


Assuntos
Antineoplásicos/isolamento & purificação , Streptomyces/química , Antineoplásicos/química , Antineoplásicos/farmacologia , Bacillus subtilis/efeitos dos fármacos , Ensaios de Seleção de Medicamentos Antitumorais , Células HCT116 , Humanos , Testes de Sensibilidade Microbiana , Estrutura Molecular , Naftoquinonas/química , Naftoquinonas/isolamento & purificação , Naftoquinonas/farmacologia
3.
Sheng Wu Gong Cheng Xue Bao ; 28(7): 865-76, 2012 Jul.
Artigo em Chinês | MEDLINE | ID: mdl-23167198

RESUMO

Wheat grain peroxidase 1 (WP1) belonged to class III plant peroxidase with cofactor heme, which not only has antifungal activity, but also influences the processing quality of flour. In order to enhance functional expression of WP1 in prokaryotic system by increasing endogenous heme synthesis, we constructed a recombinant plasmid pACYC-A-L containing hemA and hemL of Esherichia coli. Then, we co-transformed it into host strain T7 Express with secretive expression vector (pMAL-p4x-WP1) or non-secretive expression vector (pET21a-MBP-WP1), respectively. The MBP-WP1 fusion protein was further purified by amylose affinity chromatography and its peroxidase activity was assayed using 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonate) (ABTS) as substrate. At 12 h after induction at 28 degree, the extracellular 5-aminolevulinic acid (5-ALA) production of T7 Express/pACYC-A-L was up to 146.73 mg/L, simultaneously the extracellular porphrins also increased dramatically. The peroxidase activity of functional MBP-WP1 obtained from T7 Express/ (pACYC-A-L + pMAL-p4x-WP1) was 14.6-folds of that purified from T7 Express/ pET21a-MBP-WP1. This study not only successfully enhanced functional expression of wheat peroxidase 1 in Esherichia coli, but also provided beneficial references for other important proteins with cofactor heme.


Assuntos
Escherichia coli/genética , Heme/genética , Peroxidases/biossíntese , Proteínas Recombinantes de Fusão/biossíntese , Escherichia coli/metabolismo , Vetores Genéticos/genética , Heme/biossíntese , Peroxidases/genética , Proteínas Recombinantes de Fusão/genética , Transformação Genética
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA