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1.
Chempluschem ; 86(12): 1570-1576, 2021 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-34851049

RESUMO

This work reports a simple and scalable strategy to prepare a series of thermoresponsive polyurethanes synthesized via copolymerization of dicyclohexyl diisocyanate with glycerol ethoxylate in a single one-pot system. These polyurethanes exhibit lower critical solution temperatures (LCST) at 57 °C. The LCST of synthesized polyurethane was determined from Dynamic Scanning Calorimetry and UV-vis measurements. Both the LCST and Tg of synthesized polyurethane was tuned by varying the ratio between hard segment (dicyclohexyl diisocyanate) and soft segment (glycerol ethoxylate). Thus, Tg values could be tuned from -54.6 °C to -19.9 °C for samples with different flexibility. The swelling and deswelling studies were done at room temperature and above the LCST respectively. The results showed that the swelling ratio increases with the increase of soft segment (glycerol ethoxylate) in synthesized polyurethanes. Furthermore, the mechanical properties of the membrane were studied by universal tensile testing measurements. Specifically, stress at break values varied from 0.35±0.07 MPa to 0.91±0.15 MPa for the tested membranes, whereas elongation at break data ranged from 101.9±20.9 % to 192.4±24.4 %, and Young's modulus varied from 0.35±0.03 MPa to 1.85±0.19 MPa. Tensile strength of the films increased with the increase of the hard segment and elongation at break decreased.

2.
Mol Neurobiol ; 56(9): 6551-6565, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-30868446

RESUMO

The amyloid cascade hypothesis dealing with the senile plaques is until date thought to be one of the causative pathways leading to the pathophysiology of Alzheimer's disease (AD). Though many aggregation inhibitors of misfolded amyloid beta (Aß42) peptide have failed in clinical trials, there are some positive aspects of the designed therapeutic peptides for diseases involving proteinaceous aggregation. Here, we evaluated a smart design of side chain tripeptide (Leu-Val-Phe)-based polymeric inhibitor addressing the fundamental hydrophobic amino acid stretch "Lys-Leu-Val-Phe-Phe-Ala" (KLVFFA) of the Aß42 peptide. The in vitro analyses performed through the thioflavin T (ThT) fluorescence assay, infrared spectroscopy, isothermal calorimetry, cytotoxicity experiments, and so on evinced a promising path towards the development of new age AD therapeutics targeting the inhibition of misfolded Aß42 peptide fibrillization. The in silico simulations done contoured the mechanism of drug action of the present block copolymer as the competitive inhibition of aggregate-prone hydrophobic stretch of Aß42. Graphical abstract The production of misfolded Aß42 peptide from amyloid precursor protein initiates amyloidosis pathway which ends with the deposition of fibrils via the oligomerization and aggregation of Aß42 monomers. The side chain tripeptide-based PEGylated polymer targets these Aß42 monomers and oligomers inhibiting their aggregation. This block copolymer also binds and helps degrading the preformed fibrils of Aß42.


Assuntos
Doença de Alzheimer/tratamento farmacológico , Polietilenoglicóis/química , Peptídeos beta-Amiloides/química , Peptídeos beta-Amiloides/metabolismo , Peptídeos beta-Amiloides/ultraestrutura , Morte Celular , Linhagem Celular Tumoral , Sobrevivência Celular , Humanos , Ligantes , Simulação de Dinâmica Molecular , Polietilenoglicóis/síntese química , Eletricidade Estática
3.
Langmuir ; 33(40): 10588-10597, 2017 10 10.
Artigo em Inglês | MEDLINE | ID: mdl-28918640

RESUMO

Supramolecular host-guest interactions between randomly methylated ß-cyclodextrin (RM ß-CD) and side-chain phenylalanine (Phe) and Phe-Phe dipeptide-based homopolymers have been employed for the amplification of fluorescence emission of otherwise weakly fluorescent amino acid Phe. The host-guest complex has been characterized by 1H and 13C NMR spectroscopy, two-dimensional rotating-frame overhauser spectroscopy, Fourier-transform infrared spectroscopy, UV-visible spectroscopy, and fluorescence spectroscopy. To gain insights into the origin of fluorescence in homopolymers, density functional theory calculations were performed where phenyl moieties inside the less polar core of ß-CD were observed to form a π-π coupled complex resulting in an enhanced emission. Furthermore, the complex-forming ability of Phe, the guest molecule, has been employed in tuning the cloud point temperature (TCP) of statistical copolymers derived from side-chain Phe/Phe-Phe-based methacrylate monomers and N-isopropylacrylamide. By varying the co-monomer feed ratios in the statistical copolymer and hence the concentration of RM ß-CD throughout the polymer chain, host-guest interaction-assisted broad tunability in TCP of the supramolecular polymeric complex has been achieved.


Assuntos
Fenilalanina/química , Substâncias Macromoleculares , Polímeros , Espectrometria de Fluorescência , Temperatura
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