Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 11 de 11
Filtrar
Mais filtros










Base de dados
Intervalo de ano de publicação
1.
Ther Adv Infect Dis ; 8: 2049936121998548, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33796289

RESUMO

Background: Cleaning is a major control component for outbreaks of infection. However, for the SARS-CoV-2 pandemic, there is limited specific guidance regarding the proper disinfection methods that should be used. Methods: We conducted a systematic review of the literature on cleaning, disinfection or decontamination methods in the prevention of SARS-CoV-2. Results: A total of 27 studies were included, reporting a variety of methods with which the effectiveness of interventions were assessed. Virus was inoculated onto different types of material including masks, nasopharyngeal swabs, serum, laboratory plates and simulated saliva, tears or nasal fluid and then interventions were applied in an attempt to eliminate the virus including chemical, ultraviolet (UV) light irradiation, and heat and humidity. At body temperature (37°C) there is evidence that the virus will not be detectable after 2 days but this can be reduced to non-detection at 30 min at 56°C, 15 min at 65°C and 2 min at 98°C. Different experimental methods testing UV light have shown that it can inactivate the virus. Light of 254-365 nm has been used, including simulated sunlight. Many chemical agents including bleach, hand sanitiser, hand wash, soap, ethanol, isopropanol, guandinium thiocynate/t-octylphenoxypolyethoxyethanol, formaldehyde, povidone-iodine, 0.05% chlorhexidine, 0.1% benzalkonium chloride, acidic electrolysed water, Clyraguard copper iodine complex and hydrogen peroxide vapour have been shown to disinfect SARS-CoV-2. Conclusions: Heating, UV light irradiation and chemicals can be used to inactivate SARS-CoV-2 but there is insufficient evidence to support one measure over others in clinical practice.

2.
Sensors (Basel) ; 21(5)2021 Feb 25.
Artigo em Inglês | MEDLINE | ID: mdl-33668960

RESUMO

In this paper, an incorporated bridge-type superconducting fault current limiter (BSFCL) and Dynamic Voltage Restorer (DVR) is presented to improve the voltage quality and limiting fault current problems in distribution systems. In order to achieve these capabilities, the BSFCL and DVR are integrated through a common DC link as a BSFCL-DVR system. The FCL and DVR ports of the BSFCL-DVR system are located in the beginning and end of the sensitive loads' feeder integrated to the point of common coupling (PCC) in the distribution system. At first, the principle operation of the BSFCL-DVR is discussed. Then, a control system for the BSFCL-DVR system is designed to enhance the voltage quality and limit the fault current. Eventually, the efficiency of the BSFCL-DVR system is verified through the PSCAD/EMTDC simulation.

3.
World J Plast Surg ; 8(2): 245-248, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-31309063

RESUMO

BACKGROUND: Basal cell carcinomas (BCCs) are locally invasive periocular skin cancers affecting lower eyelids more than upper eyelids. The purpose of this study was to describe techniques used for lower eyelid reconstruction after extended excision of BCC. METHODS: Eight referred patients with BCC who underwent lower eyelid reconstruction were enrolled. The tumor was surgically excised with sufficient margins by one surgeon. Defects were repaired by subdermal tunnel between lateral border of defect and insertion site of lateral cantus. RESULTS: Eight patients aged 45 to 75 years were followed up for 6 months. After follow up, adequate viability of the grafts, satisfactory functional and good cosmetic results was noticed in all patients. One patient complained of irritation at the site of surgery. No total or partial necrosis, hematoma, or infection were observed in flaps, and no additional surgery was needed. CONCLUSION: The present novel surgical procedure was useful to close full thickness defects in the lower lid to preserve the function of the lower eyelid and a good aesthetic outcome.

4.
World J Plast Surg ; 8(2): 249-253, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-31309064

RESUMO

BACKGROUND: Microvascular surgery is one of the most important parts of reconstructive surgery. In the present study, the effect of ethanol on microvascular diameter and prevention of thrombosis was evaluated. METHODS: Totally, 80 vessels including 40 arteries and 40 veins in right and left ears of 20 adult rabbits were enrolled. Seven days after injection of ethanol to rabbit ear vessel, vessel diameter and thrombosis rate post-iced saline challenge were documented and compared to normal saline injection in contralateral ear as a control group. RESULTS: Vessel diameter in both arteries and veins in ethanol group was significantly larger than normal saline control group, and patency rates due to preventive effect of ethanol were also significantly higher in the ethanol group after iced saline challenge. CONCLUSION: Pretreatment with ethanol can enlarge vessel diameter and play a preventive role on thrombosis after iced saline challenge.

5.
Vet Parasitol ; 266: 84-87, 2019 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-30736953

RESUMO

Cystic echinococcosis is of great public health importance. Surgery is the efficient treatment for this infection. To minimize the risk of cyst intraoperative leakage, using scolicidals is crucial. To date, any efficacious scolicidal without side effect has not been introduced. Since essential oils of Pelargonium roseum and Ferula gummosa have shown several bioactivities, we evaluated their potential against protoscoleces of E. granulosus using albendazole as positive control. Furthermore, chemical composition of both essential oils was analyzed by gas chromatography-mass spectrometry (GC-MS) analyses, and their main constituents were also evaluated for scolicidal activity. Different concentrations of essential oils and their two main constituents were tested for scolicidal activity. Mortality rate was measured by eosin staining. Results of GC-MS revealed citronellol and ß-pinene as the main constituents of P. roseum and F. gummosa essential oils, respectively. After 60 min of exposure to 50 µg/mL of P. roseum and F. gummosa, mean mortality rate of protoscoleces was 100%. However, ß-pinene and citronellol at the same time point with only 10 µg/mL concentrations resulted in approximately higher than 80% mortality. Essential oils of P. roseum and F. gummosa showed significant toxic effect on E. granulosus with 50% lethal concentration (LC50) values of 8.52 and 17.18 µg/mL, respectively. Based on the LC50 values, ß-pinene (2.20 µg/mL) was the most potent scolicidal agent in the present study. The overall toxicity of ß-pinene and citronellol was significantly higher than the whole essential oils of F. gummosa and P. roseum. Based on these results, ß-pinene and citronellol can be considered as candidate ingredients for the development of green scolicidals.


Assuntos
Echinococcus granulosus/efeitos dos fármacos , Ferula/química , Óleos Voláteis/farmacologia , Pelargonium/química , Óleos Vegetais/farmacologia , Monoterpenos Acíclicos , Albendazol/farmacologia , Animais , Monoterpenos Bicíclicos , Compostos Bicíclicos com Pontes/farmacologia , Cromatografia Gasosa-Espectrometria de Massas , Monoterpenos/farmacologia
6.
J Theor Biol ; 368: 122-32, 2015 Mar 07.
Artigo em Inglês | MEDLINE | ID: mdl-25591889

RESUMO

For the first time, prediction accuracies of some supervised and unsupervised algorithms were evaluated in an SSR-based DNA fingerprinting study of a pea collection containing 20 cultivars and 57 wild samples. In general, according to the 10 attribute weighting models, the SSR alleles of PEAPHTAP-2 and PSBLOX13.2-1 were the two most important attributes to generate discrimination among eight different species and subspecies of genus Pisum. In addition, K-Medoids unsupervised clustering run on Chi squared dataset exhibited the best prediction accuracy (83.12%), while the lowest accuracy (25.97%) gained as K-Means model ran on FCdb database. Irrespective of some fluctuations, the overall accuracies of tree induction models were significantly high for many algorithms, and the attributes PSBLOX13.2-3 and PEAPHTAP could successfully detach Pisum fulvum accessions and cultivars from the others when two selected decision trees were taken into account. Meanwhile, the other used supervised algorithms exhibited overall reliable accuracies, even though in some rare cases, they gave us low amounts of accuracies. Our results, altogether, demonstrate promising applications of both supervised and unsupervised algorithms to provide suitable data mining tools regarding accurate fingerprinting of different species and subspecies of genus Pisum, as a fundamental priority task in breeding programs of the crop.


Assuntos
Genes de Plantas , Modelos Genéticos , Ervilhas/genética , Algoritmos , Teorema de Bayes , Análise por Conglomerados , Impressões Digitais de DNA/métodos , DNA de Plantas/genética , Árvores de Decisões , Marcadores Genéticos , Genótipo , Repetições de Microssatélites , Especificidade da Espécie , Máquina de Vetores de Suporte
7.
Artigo em Inglês | MEDLINE | ID: mdl-24124688

RESUMO

Seven ab initio web-based gene prediction programs (i.e., AUGUSTUS, BGF, Fgenesh, Fgenesh+, GeneID, Genemark.hmm, and HMMgene) were assessed to compare their prediction accuracy using protein-coding sequences of bread wheat. At both nucleotide and exon levels, Fgenesh+ was deduced as the superior program and BGF followed by Fgenesh were resided in the next positions, respectively. Conversely, at gene level, Fgenesh with the value of predicting more than 75% of all the genes precisely, concluded as the best ones. It was also found out that programs such as Fgenesh+, BGF, and Fgenesh, because of harboring the highest percentage of correct predictive exons appear to be much more applicable in achieving more trustworthy results, while using both GeneID and HMMgene the percentage of false negatives would be expected to enhance. Regarding initial exon, overall, the frequency of accurate recognition of 3' boundary was significantly higher than that of 5' and the reverse was true if terminal exon is taken into account. Lastly, HMMgene and Genemark.hmm, overall, presented independent tendency against GC content, while the others appear to be slightly more sensitive if GC-poor sequences are employed. Our results, overall, exhibited that to make adequate opportunity in acquiring remarkable results, gene finders still need additional improvements.


Assuntos
Pão , Biologia Computacional/métodos , Genes de Plantas/genética , Software , Triticum/genética , Composição de Bases/genética , Éxons/genética , Internet , Oligodesoxirribonucleotídeos/genética , Reação em Cadeia da Polimerase
8.
Appl Biochem Biotechnol ; 168(4): 770-84, 2012 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-22907514

RESUMO

To diminish the time required for some diagnostic assays including reverse transcription PCR (RT-PCR), reverse transcription loop-mediated isothermal amplification (RT-LAMP; due to mainly RNA extraction step) and also DAS-ELISA into a minimum level, an innovative immunocapture RT-LAMP (IC-RT-LAMP) and immunocapture reverse transcription (IC/RT-PCR) protocol on the basis of Potato Leafroll virus (PLRV) genome were used and optimized. In this regard, all six IC-RT-LAMP primers (i.e. F3, B3, FIP, BIP, LF and LB) together with IC/RT-PCR primers were designed on the basis of the highly conserved sequence (ORF3) of coat protein gene (GenBank accession number: U73777) of PLRV genome. Even though DAS-ELISA, IC/RT-PCR and IC-RT-LAMP assays could successfully detect positive infected plant samples, considering the time, safety, sensitivity, cost and simplicity, the last one was overall superior. Meanwhile, among five different visual dyes to accurately detect IC-RT-LAMP products, both hydroxynaphthol blue and GeneFinder™ could produce long stable colour change and brightness in a close tube-based approach to prevent cross-contamination risk, concluded eventually as the best ones. Altogether, as IC-RT-LAMP is sensitive, cost-effective, fairly user friendly and also can generate more accurate results than previous diagnostic procedures, we accordingly propose this colorimetric assay as a highly reliable alternative viral recognition system regarding PLRV recognition and probably other viral-based diseases.


Assuntos
Luteoviridae/isolamento & purificação , Imagem Molecular/métodos , Cor , Ensaio de Imunoadsorção Enzimática , Genoma Viral/genética , Luteoviridae/genética , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Segurança
9.
Phys Rev E Stat Nonlin Soft Matter Phys ; 85(4 Pt 1): 041102, 2012 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-22680415

RESUMO

We study a nanoscale system composed of one corrugated plate (rack) and one corrugated cylinder (pinion) coupled via the lateral Casimir force. We assume that the rack moves uniformly. The axle of the pinion experiences frictional torque and random torque due to a surrounding fluid. We show that even in the presence of thermal noise, the device can work against external loads: The pinion rotates with a nonzero average velocity. The device operation becomes less influenced by the noise as the gap between rack and pinion decreases.


Assuntos
Microfluídica/métodos , Modelos Químicos , Modelos Moleculares , Proteínas Motores Moleculares/química , Transdutores , Simulação por Computador , Transferência de Energia , Desenho de Equipamento , Análise de Falha de Equipamento , Temperatura Alta , Termodinâmica
10.
Phys Rev E Stat Nonlin Soft Matter Phys ; 82(1 Pt 2): 016117, 2010 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-20866699

RESUMO

We study a nanoscale system composed of one corrugated cylinder (pinion) placed between two corrugated plates (racks). The pinion and racks have no mechanical contact, but are coupled via the lateral Casimir force-one of the most spectacular consequences of quantum fluctuations of the electromagnetic field. The noncontact design of the device could help with the noteworthy wear problem in nanoscale mechanical systems. We consider the case where both racks undergo harmonic lateral motion. We assume that the amplitude, frequency, and phase of one of the racks are known. We show that probing the pinion motion, one can determine the vibration characteristics of the other rack.

11.
Phys Rev E Stat Nonlin Soft Matter Phys ; 82(3 Pt 2): 037101, 2010 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-21230211

RESUMO

We study a nanoscale system composed of one corrugated cylinder (pinion) and one corrugated plate (rack). The pinion and rack have no mechanical contact, but are coupled via the lateral Casimir force. We consider the case where the rack position versus time is a periodic triangular signal. We find that the device can rectify the periodic but nonsinusoidal motion of the rack. Using the typical values of parameters, we find that the pinion rotates with an average angular velocity Ω=1∼100 Hz . Experimental observation of the pinion rotation will show that the quantum vacuum can intermesh the noncontact parts of nanomachines.

SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...