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1.
Daru ; 2020 Aug 18.
Artigo em Inglês | MEDLINE | ID: mdl-32812187

RESUMO

PURPOSE: A recent survey has shown that the COVID-19 pandemic has culminated in dramatical and critical treatment particularly in acute infected patients. In fact, this systematic review-meta-analysis was directly pertained to estimation at the efficient value of some clinical managements to confront the COVID-19 infection. METHODS: Pubmed, Embase, Scopus, Cochrane, and Scholar databases were searched from inception to July 1, 2020, to identify studies reporting the current treatment process and medications (e.g. hydroxychloroquine, antiviral therapy, convalescent plasma, and immunomodulatory agents) for COVID-19. A random-effects model meta-analysis was performed to calculate the relative risk (RR) with 95% confidence intervals (CI). The outcomes of this study were the frequency of negative conversion cases, clinical improvements, mechanical ventilation demand, intensive care unit (ICU) entry, and mortality. The standard treatment refers to the published guidelines and specialist experience which varies in different articles, and the proposed treatment refers to the kind of interest suggested in the included studies. RESULTS: A number of 45 articles met the eligibility criteria (out of 6793 articles). Among them, 26 articles involving 3263 patients were included in quantitative analysis. Anti-COVID-19 interventions could significantly increase clinical improvement (RR 1.17, 95% CI 1.08-1.27; I2 = 49.8%) and reduce the mortality rate (RR 0.58, 95% CI 0.35-0.95; I2 = 74.8%). Although in terms of negative conversion, ICU entry, and mechanical ventilation demand, clinical intervention had no beneficial effect. The clinical effect of immunomodulatory agents (especially tocilizumab and anakinra) was noticeable compared to other medications with RR of 0.22 (95% CI 0.09-0.53; I2 = 40.9%) for mortality and 1.25 (95% CI 1.07-1.46; I2 = 45.4%) for clinical improvement. Moreover, Antivirals (RR 1.13, 95% CI 1.01-1.26; I2 = 47.0%) and convalescent plasma therapy (RR 1.41, 95% CI 1.01-1.98; I2 = 66.6%) had significant beneficial effects on clinical improvement. CONCLUSION: Based on our findings, all the included interventions significantly declined the mortality and enhanced clinical improvements with no effect on negative conversion and mechanical ventilation demand. Especially, immunomodulators and plasma therapy showed favorable outcomes. An evaluation on the efficacy of proposed treatment against COVID-19.

2.
Meat Sci ; 167: 108161, 2020 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-32380358

RESUMO

The objective of this study was to fabricate betanin nanoliposomes incorporated gelatin/chitosan nanofiber/ZnO nanoparticles bionanocomposite film (G/CH NF/ZnO NPs/B NLPs) and investigate its effects on the preservation of fresh beef. The scanning electron microscopy image of nanocomposite film displayed a good inter-connective porous morphology. Fourier transform infrared and X-ray diffraction analysis confirmed the formation of new hydrogen bonds and enhanced crystallinity through the addition of CH NF, ZnO NPs, and B NLPs. The G/CH NF/ZnO NPs/B NLPs film exhibited satisfactory mechanical properties and high surface hydrophobicity (water contact angle = 92.49 ± 3.71°). The incorporation of ZnO NPs and B NLPs in the nanocomposite film provided high antibacterial activity and DPPH inhibition activity (53.02 ± 3.26%). The growth of inoculated bacteria, lipid oxidation, and the changes in the pH and color quality of the beef samples were controlled by packaging with the fabricated film. In conclusion, the G/CH NF/ZnO NPs/B NLPs nanocomposite has a high potential for meat preservation.

3.
J Cell Sci ; 133(10)2020 May 27.
Artigo em Inglês | MEDLINE | ID: mdl-32327555

RESUMO

We have determined the electropharmacological properties of a new potassium channel from brain mitochondrial membrane using a planar lipid bilayer method. Our results show the presence of a channel with a conductance of 150 pS at potentials between 0 and -60 mV in 200 mM cis/50 mM trans KCl solutions. The channel was voltage independent, with an open probability value of approximately 0.6 at different voltages. ATP did not affect current amplitude or open probability at positive and negative voltages. Notably, adding iberiotoxin, charybdotoxin, lidocaine or margatoxin had no effect on the channel behavior. Similarly, no changes were observed by decreasing the cis pH to 6. Interestingly, the channel was inhibited by adding sodium in a dose-dependent manner. Our results also indicated a significant increase in mitochondrial complex IV activity and membrane potential and a decrease in complex I activity and mitochondrial ROS production in the presence of sodium ions. We propose that inhibition of mitochondrial potassium transport by sodium ions on potassium channel opening could be important for cell protection and ATP synthesis.

4.
Protoplasma ; 257(2): 459-473, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-31776775

RESUMO

Lepidium draba is a weed with the medicinal properties which few researches have been done on it. In this study, some traits, related to the osmotic stress, in 14-day-old L. draba sprouts that were grown 9 days in the presence of various doses of polyethylene glycol 6000 (PEG 6000) including 0, 3, 6, 9, and 12%, with different osmotic potentials (- 0.04, - 0.12, - 0.23, - 0.34, and - 0.48 MPa, respectively) were investigated. Based on our results, germination percentage besides stem and root lengths decreased with increasing the concentrations of PEG. The contents of electrolyte leakage, malondialdehyde, other aldehydes, total protein, free amino acids, total soluble carbohydrate as well as free proline increased with increasing the concentrations of PEG. Also, for the first time, our results have proven that under osmotic stress, there is an adverse relationship between hydrogen peroxide content and the activity of catalase, peroxidase, ascorbate peroxidase, and guaiacol peroxidase enzymes, such that hydrogen peroxide content decreased with induction of PEG up to 6% and after that increased, while the activity of catalase, peroxidase, ascorbate peroxidase, and guaiacol peroxidase enzymes increased up to 6% PEG and after that decreased. The expression levels of catalase, peroxidase, ascorbate peroxidase, and guaiacol peroxidase genes showed the same pattern as was seen for these enzyme activities. According to the results of this study, it can be deduced that decreasing H2O2 content cannot be the main reason for other oxidative stress parameters to decrease. In this study, P5CS and P5CR gene expression levels increased with increasing levels of PEG up to 12% which was completely similar to free proline content. Based on our results, L. draba can be considered as a semi-tolerant plant to osmotic stress.

5.
Mol Divers ; 2019 Dec 23.
Artigo em Inglês | MEDLINE | ID: mdl-31873869

RESUMO

Soluble epoxide hydrolase (sEH) enzyme plays an important role in the metabolism of endogenous chemical mediators, epoxyeicosatrienoic acids, which are involved in the regulation of blood pressure and inflammation. According to the pharmacophoric model suggested for sEH inhibitors, some new amide-based derivatives of 3-phenylglutaric acid were designed, synthesized and biologically evaluated. Docking study illustrated that the amide group as a primary pharmacophore had a suitable distance from the three amino acids of Tyr383, Tyr466 and Asp335 for effective hydrogen binding. Most of the compounds showed moderate to high sEH inhibitory activities in in vitro test in comparison with 12-(3-Adamantan-1-yl-ureido)-dodecanoic acid, as a potent urea-based sEH inhibitor. Compound 6o with phenethyl in R position exhibited the highest activity with IC50 value of 0.5 nM. In this study, some new amide-based derivatives of 3-phenylglutaric acid were designed, synthesized and biologically evaluated. Most of the synthesized compounds provided nanomolar range inhibition against sEH enzyme. The best observed IC50 value was 0.5 nM. Incorporating a carboxylic moiety into these structures by forming carboxylate salts would increase the solubility and improving physicochemical properties.

6.
J Biosci ; 44(4)2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31502582

RESUMO

A new and simple procedure was applied to detect bisphenol A (BPA) based on a BPA aptamer and its complementary strand (Comp. Str.). An electrode was modified with a mixture of carboxylated multiwalled carbon nanotubes and chitosan. The Comp. Str. was immobilized on a modified-glassy carbon electrode (GCE) surface via covalent binding. After the incubation of the aptamer with the electrode surface, it could interact with the Comp. Str. In the presence of BPA, its aptamer will interact with the analyte, resulting in some changes in the configuration and leading to separation from the electrode surface. Due to the attached ferrocene (Fc) group on the 50 head of the aptamer, the redox current of Fc has reduced. This aptasensor can sense the level of BPA in the linear range of 0.2-2 nM, with a limit of detection of 0.38 nM and a sensitivity of 24.51 lA/µM. The proposed aptasensor showed great reliability and selectivity. The acceptable selectivity is due to the specificity of BPA binding to its aptamer. The serum sample was used as a real sample; the aptasensor was able to effectively recover the spiked BPA amounts. It can on-site monitor the BPA in serum samples with acceptable recoveries.


Assuntos
Aptâmeros de Nucleotídeos/química , Compostos Benzidrílicos/isolamento & purificação , Técnicas Biossensoriais , Técnicas Eletroquímicas , Fenóis/isolamento & purificação , Aptâmeros de Nucleotídeos/genética , Compostos Benzidrílicos/sangue , Quitosana/química , Eletrodos , Compostos Ferrosos/química , Humanos , Metalocenos/química , Nanotubos de Carbono/química , Fenóis/sangue
7.
Chem Commun (Camb) ; 55(74): 11095-11098, 2019 Sep 21.
Artigo em Inglês | MEDLINE | ID: mdl-31460525

RESUMO

The electrophilic borylation of 2,5-diarylpyrazines results in the formation of boron-nitrogen doped dihydroindeno[1,2-b]fluorene which can be synthesized using standard Schlenk techniques and worked up and handled readily under atmospheric conditions. Through transmetallation via diarylzinc reagents a series of derivatives were synthesized which show broad visible to near-IR light absorption profiles that highlight the versatility of this BN substituted core for use in optoelectronic devices. The synthesis is efficient, scalable and allows for tuning through changes in substituents on the planar heterocyclic core and at boron. Exploratory evaluation in organic solar cell devices as non-fullerene acceptors gave power conversion efficiencies of 2%.

8.
Biotechnol Appl Biochem ; 66(4): 502-509, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-30919496

RESUMO

In this study, the simple, green, and fast layer-by-layer modification of the glassy carbon electrode was mainly performed by electrodeposition of gold nanoparticles and then, poly-l-arginine, and finally, laccase was covalently bonded to poly-l-arginine using glutaraldehyde. This type of fabrication is used for the first time for catechol detection, which provides a bioelectrocatalytic cycle for electron transport in the presence of laccase that results in sensitive and fast detection of catechol. The scanning electron microscopy, Fourier-transform infrared spectroscopy, and electrochemical studies were performed to confirm successful immobilization of the enzyme. The biosensor response was linear in a wide range of catechol trace concentrations, 24.90-274.00 nM, with the detection limit of 18.00 nM. Values of Km , α, n, and Ks for the immobilized enzyme were calculated to be 1.25 × 10-2  µM, 0.56, 3.19, and 0.28 Sec-1 , respectively. It was examined in real sample successfully confirming it is capable of measuring catechol in natural water.


Assuntos
Técnicas Biossensoriais , Catecóis/análise , Ouro/química , Lacase/química , Nanopartículas Metálicas/química , Peptídeos/química , Poluentes Químicos da Água/química , Lacase/metabolismo , Tamanho da Partícula , Propriedades de Superfície
9.
J Genet ; 97(5): 1433-1444, 2018 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-30555091

RESUMO

Eight candidate housekeeping genes were examined as internal controls for normalizing expression analysis of durum wheat (Triticum durum L.) under drought and salinity stress conditions. Quantitative real-time PCR was used to analyse gene expression of multiple stress levels, plant ages (24 and 50 days old), and plant tissues (leaf and root). The algorithms BestKeeper, NormFinder, GeNorm, the delta Ct method and the RefFinder were applied to determine the stability of candidate genes. Under drought stress, the most stable reference genes were glyceraldehyde-3 phosphate, ubiquitin and ß-tubulin2, whereas under salinity stress conditions, eukaryotic elongation factor 1-α, glyceraldehyde-3 phosphate and actin were identified as the most stable reference genes. Validation with stress-responsive genes NAC29 and NAC6 demonstrated that the expression level of target genes could be determined reliably with combinations of up to three of the reference genes. This is the first report on reference genes appropriate for quantification of target gene expression in T. durum under drought and salt stresses. Results of this investigation may be applicable to other Triticum species.


Assuntos
Perfilação da Expressão Gênica/métodos , Regulação da Expressão Gênica de Plantas , Genes Essenciais/genética , Genes de Plantas/genética , Reação em Cadeia da Polimerase Via Transcriptase Reversa/métodos , Triticum/genética , Algoritmos , Secas , Perfilação da Expressão Gênica/normas , Padrões de Referência , Reprodutibilidade dos Testes , Reação em Cadeia da Polimerase Via Transcriptase Reversa/normas , Estresse Salino , Estresse Fisiológico
10.
Adv Clin Exp Med ; 27(11): 1477-1482, 2018 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-30411548

RESUMO

BACKGROUND: Conjugated linoleic acid (CLA), which is an octadecadienoic acid isomer, is believed to play different positive physiological roles, such as lowering body fat. Due to some reported side effects of CLA, like lipodystrophy and impaired glucose metabolism, it is important to establish its safety by understanding detailed molecular mechanisms. One of these mechanisms may be the role of this dietary agent in modifying the function and activity of microRNAs (miRNAs). OBJECTIVES: The aim of the study was to investigate how adipocyte miR-27a and miR-143 expression may be influenced by CLA in obese rats. MATERIAL AND METHODS: In this study, 24 male Wistar rats were randomly divided into normal-fat diet (NFD) and high-fat diet (HFD) groups. After 8 weeks, the rats were weighed and half of the diet-induced obese rats were randomly selected to receive 500 mg CLA per 1 kg body weight for 4 weeks. At the end of this period, epididymal fat was isolated to investigate the expression level of miRNAs by real-time polymerase chain reaction (RT-PCR). RESULTS: After 12 weeks, the obese rats in the HFD group, compared with rats in the NFD group, demonstrated a significant decrease in the expression of miR-27a (p < 0.05) and a significant increase in the expression of miR-143 (p < 0.05). In the group which had received CLA for a 4-week period, these events were reversed. Moreover, the rats in this group gained less weight than other rats in HFD groups, although the difference was not statistically significant. CONCLUSIONS: In conclusion, this study demonstrated that CLA, as an anti-obesity agent, may minimize abnormal changes in miRNA expression in obesity. This suggests a new pathway for weight loss; however, further studies are needed.


Assuntos
Tecido Adiposo/metabolismo , Dieta , Expressão Gênica/efeitos dos fármacos , Ácidos Linoleicos Conjugados/farmacologia , MicroRNAs/efeitos dos fármacos , Obesidade/genética , Tecido Adiposo/efeitos dos fármacos , Animais , Gorduras na Dieta/administração & dosagem , Masculino , Obesidade/etiologia , Distribuição Aleatória , Ratos , Ratos Wistar
11.
Plant Physiol Biochem ; 132: 445-452, 2018 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-30292161

RESUMO

Moisture stress is the most important limitation of wheat production in the worldwide. Among the tribe Triticeae, Aegilops tauschii is one of the most valuable gene sources of resistance to abiotic stresses. In order to identify the most tolerant accession to moisture stress, and to understand its adaptive mechanisms at the molecular level, the present experiment was carried out on ten Ae. tauschii accessions under normal (95% soil pot capacity) and moisture stress (45% soil pot capacity) conditions. At the start of the heading time, the expanded flag leaves of treated and untreated plants were sampled for two-dimensional electrophoresis (2-DE) based on proteomics approach. A19 accession was less affected by the imposed moisture stress; therefore, it was used for the proteomics experiment. Among 252 protein spots which were reproducibly detected in each given 2-DE gels, 25 spots showed significant differences between the two moisture treatments; 17 spots were upregulated and 8 spots were downregulated. The identified proteins by MALDI-TOF/TOF, were allocated to seven functional protein groups, which were mainly involved in photosynthesis/respiration (28.5%), carbohydrate metabolism (14.2%), energy metabolism (7.1%), chaperone (14.2%), protein translation and processing (14.2%), repair and stability of the genome (7.1%) and unknown function (14.2%). We report this for the first time that RMI2 protein (in the group of repair and stability of the genome) was significantly changed in wheat in response to moisture stress. We believe that, the identified proteins could play important roles in acclimation and tolerance to moisture stress and provide the genetic pathways for improving tolerance to moisture stress in wheat.


Assuntos
Umidade , Poaceae/anatomia & histologia , Poaceae/fisiologia , Proteômica/métodos , Estresse Fisiológico , Adaptação Fisiológica , Ecótipo , Eletroforese em Gel Bidimensional , Proteínas de Plantas/metabolismo , Análise de Componente Principal , Proteoma/metabolismo , Estações do Ano , Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz
12.
Curr Comput Aided Drug Des ; 14(4): 391-397, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-30047333

RESUMO

INTRODUCTION: Acetylcholinesterase enzyme (AChE) is the main target in Alzheimer's disease therapy and designing of novel AChE inhibitors is a great deal of attention. METHODS: In this study, 2D-QSAR and 3D-QSAR models were generated using stepwise multiple linear regressions (SW-MLR) and comparative molecular field analysis (CoMFA) respectively. RESULTS: It was found that CoMFA model with r2 of 0.947 for the training set and r2 of 0.816 for the test set is more favorable than model which is established by SW-MLR method with r2 =0.825 and r2pred =0.778 in 2D-QSAR. CONCLUSION: In addition, obtaining models were validated by cross validation with cut off values of q2 > 0.5 and r2pred > 0.6.


Assuntos
Inibidores da Colinesterase/química , Inibidores da Colinesterase/farmacologia , Piperazina/química , Piperazina/farmacologia , Piperidinas/química , Piperidinas/farmacologia , Acetilcolinesterase/metabolismo , Doença de Alzheimer/tratamento farmacológico , Doença de Alzheimer/enzimologia , Humanos , Modelos Moleculares , Terapia de Alvo Molecular , Relação Quantitativa Estrutura-Atividade
13.
J Cancer Res Ther ; 14(3): 503-508, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-29893306

RESUMO

Context: It has been shown that hesperidin induces apoptosis in NALM-6 cells through inhibition of nuclear factor-kappa B (NF-κB) activation. Aims: To investigate the effect of hesperidin on inhibition of NF-κB activation through blocking phosphoinositide 3-kinase (PI3K)/Akt pathway as a main target in cancer treatment, in NALM-6 cells. Materials and Methods: NALM-6 cells were incubated with two concentrations of hesperidin (25, 50 µM) in the presence or absence of insulin (100 nM), as a potent activator of Akt. The cytotoxic activity of hesperidin was determined by 3-(4,5-methylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Cell apoptotic death was measured by ELISA test using cell death detection ELISA Plus kit. To assay the effect of hesperidin on Akt pathway, the phosphorylation levels of Akt, inhibitor of kappa B alpha (IκBα), and glycogen synthase kinase-3 beta (GSK-3ß) and expression level of IκB kinase alpha (IKKα) were determined by Western blot analysis. Results: Hesperidin (both concentrations) significantly reduced cells survival in the presence and absence of insulin compared to untreated cells in a time-dependent manner (P < 0.05). Hesperidin also significantly increased apoptosis in NALM-6 cells even in hyperinsulinemia condition (P < 0.0001). Hesperidin inhibited insulin-induced phosphorylation and activation of Akt, IκBα, and GSK-3ß and decreased expression of IKKα. Conclusion: The results of this study demonstrated that cytotoxic and proapoptotic actions of hesperidin are partly mediated through the suppression of PI3K3/Akt/IKK signaling pathway. So, hesperidin might act as a chemotherapeutic agent by targeting cell survival pathways.


Assuntos
Apoptose/efeitos dos fármacos , Hesperidina/farmacologia , Insulina/genética , Neoplasias/tratamento farmacológico , Antineoplásicos/farmacologia , Sobrevivência Celular/efeitos dos fármacos , Regulação Neoplásica da Expressão Gênica/efeitos dos fármacos , Glicogênio Sintase Quinase 3 beta/genética , Humanos , Insulina/metabolismo , Inibidor de NF-kappaB alfa/genética , NF-kappa B/antagonistas & inibidores , NF-kappa B/genética , Neoplasias/genética , Neoplasias/patologia , Fosfatidilinositol 3-Quinases/genética , Fosforilação/efeitos dos fármacos , Fosforilação/genética , Células Precursoras de Linfócitos B/efeitos dos fármacos , Células Precursoras de Linfócitos B/patologia , Proteínas Proto-Oncogênicas c-akt/genética , Transdução de Sinais/efeitos dos fármacos
14.
Basic Clin Neurosci ; 8(5): 405-411, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-29167727

RESUMO

Introduction: Balance impairment is a common problem and a major cause of motor disability after stroke. Therefore, this study aimed to investigate whether low-frequency repetitive Transcranial Magnetic Stimulation (rTMS) improves the postural balance problems in stroke patients. Methods: This randomized double blind clinical trial with 12 weeks follow-up was conducted on stroke patients. Treatment was carried with 1 Hz rTMS in contralateral brain hemisphere over the primary motor area for 20 minutes (1200 pulses) for 5 consecutive days. Static postural stability, Medical Research Council (MRC), Berg Balance Scale (BBS), and Fugl-Meyer assessments were evaluated immediately, 3 weeks and 12 weeks after intervention. Results: A total of 26 patients were enrolled (age range=53 to 79 years; 61.5% were male) in this study. Administering rTMS produced a significant recovery based on BBS (df=86, 7; F=7.4; P=0.01), Fugl-Meyer Scale (df=86, 7; F=8.7; P<0.001), MRC score (df=87, 7; F=2.9; P=0.01), and static postural stability (df=87, 7; F=9.8; P<0.001) during the 12 weeks follow-up. Conclusion: According to the findings, rTMS as an adjuvant therapy may improve the static postural stability, falling risk, coordination, motor recovery, and muscle strength in patients with stroke.

15.
Cryobiology ; 78: 34-40, 2017 10.
Artigo em Inglês | MEDLINE | ID: mdl-28716598

RESUMO

This investigation was carried out to study the effect of soybean lecithin 1.5% (wt/vol) (0, 2.5, 5 and 7.5 mg l-1 pomegranate extract (PE)) or PE-loaded lecithin nanoliposome (0, 2.5, 5 and 7.5 mg l-1) to Tris-based extender. Sperm motility (CASA), viability, membrane integrity (HOS test), abnormalities, mitochondrial activity, apoptosis status, lipid peroxidation, total antioxidant capacity (TAC)) and antioxidant activities (GPX, SOD) were investigated following freeze-thawing. No significant differences were detected in motility parameters, viability, membrane integrity, and mitochondria activity after thawing sperm between soybean lecithin and lecithin nanoliposomes. It was shown that PE5 significantly improved sperm total and progressive motility, membrane integrity, viability, mitochondria activity, TAC and reduced lipid peroxidation (malondialdehyde concentration). Moreover, the percentage of apoptotic sperm in PE5 extenders was significantly the lowest among other treatments. Sperm abnormalities, SOD and GPX were not affected by the antioxidant supplements. For apoptotic status, no differences were observed between soybean lecithin and lecithin nanoliposome. We showed that lecithin nanoliposome extender can be a beneficial alternative extender to protect ram sperm during cryopreservation without any adverse effects. It was also observed that regarding pomegranate concentration, PE5 can improve the quality of ram semen after thawing.


Assuntos
Criopreservação/métodos , Crioprotetores/farmacologia , Lythraceae/metabolismo , Extratos Vegetais/farmacologia , Lectinas de Plantas/farmacologia , Preservação do Sêmen/métodos , Proteínas de Soja/farmacologia , Animais , Antioxidantes/farmacologia , Membrana Celular/efeitos dos fármacos , Sobrevivência Celular/efeitos dos fármacos , Citometria de Fluxo , Peroxidação de Lipídeos/efeitos dos fármacos , Lipossomos/química , Lipossomos/farmacologia , Masculino , Malondialdeído/análise , Mitocôndrias/efeitos dos fármacos , Oxirredução/efeitos dos fármacos , Sêmen/efeitos dos fármacos , Ovinos , Soja/metabolismo , Motilidade Espermática/efeitos dos fármacos , Espermatozoides/efeitos dos fármacos
16.
J Spinal Cord Med ; 40(2): 201-212, 2017 03.
Artigo em Inglês | MEDLINE | ID: mdl-26679398

RESUMO

OBJECTIVE: The probable effect of dietary amino acids intake pattern on serum lipid profile and blood pressure (BP) have not yet been described among individuals with spinal cord injury (SCI). DESIGN: Cross-sectional. SETTING: Tertiary rehabilitation center. PARTICIPANTS: People with SCI referred to Brain and Spinal Cord Injury Research Center between 2011 and 2014. OUTCOME MEASURES: Dietary intakes were assessed by recording consumed foods by 24-hour dietary recall interviews using Nutritionist IV 3.5.3 modified for Iranian foods. Partial correlation test with adjustment for age, weight, body mass index, total energy intake, total fat, cholesterol and carbohydrate intake, and injury-related variables was used. RESULTS: Dietary intake of lysine was positively related to levels of fasting plasma glucose (FPG), triglyceride (TG), systolic blood pressure (SBP) and diastolic blood pressure (DBP) (P < 0.0001, 0.046, 0.002 and 0.009, respectively). There was a positive significant relationship between the intake of cysteine and levels of TG and SBP as well (P : 0.027 and 0.048, respectively). Higher intakes of threonine and leucine had a negative relationship with TG level (P : 0.001 and 0.026, respectively). Furthermore, tyrosine, threonine and leucine were inversely correlated to blood pressure. Total cholesterol level was only related to intake of threonine and leucine (P : 0.004 and 0.012, respectively). FPG was positively associated with intake of all amino acids except for cysteine, glutamic acid, threonine, leucine and histidine. CONCLUSION: In the present study, the pattern of relationships between dietary intake of amino acids and serum lipid profile and BP has been described among people with SCI.


Assuntos
Pressão Sanguínea , Proteínas na Dieta/metabolismo , Lipídeos/sangue , Lisina/metabolismo , Traumatismos da Medula Espinal/sangue , Adulto , Glicemia/metabolismo , Proteínas na Dieta/administração & dosagem , Proteínas na Dieta/efeitos adversos , Feminino , Humanos , Lisina/administração & dosagem , Lisina/efeitos adversos , Masculino , Pessoa de Meia-Idade , Traumatismos da Medula Espinal/metabolismo , Traumatismos da Medula Espinal/fisiopatologia
17.
Synapse ; 70(9): 351-60, 2016 09.
Artigo em Inglês | MEDLINE | ID: mdl-27124112

RESUMO

OBJECTIVE: The subthreshold brain-damaging stimulus may protect the brain from subsequent ischemia; this phenomenon has been named "ischemic tolerance" (IT). We focused on the synaptic properties of the neurons after mild and severe ischemia to determine the association between IT and synaptic efficacy. EXPERIMENTAL DESIGN: Adult male rats were randomly divided into four experimental groups including control, sham, permanent ischemia (pI/R), and mild ischemia (mI/R). Middle cerebral artery occlusion (MCAO) method was applied to induce brain ischemia. Seven days after the insult, long-term potentiation (LTP) induced by high-frequency stimulation (HFS) and paired-pulse ratio (PPR) were monitored before and after the HFS delivery. RESULTS: The field potential recording demonstrated that mild ischemia significantly increased the basal synaptic transmission. Additionally, the HFS produced a significant potentiation compared to its baseline level in the mI/R group. Moreover, mild ischemia prevented depression of PPR by HFS. This effect was accompanied by a significant increase in the normalized PPR (PPR after HFS/PPR before HFS) in this group. CONCLUSIONS: Our data indicated that a mild reduction in brain perfusion without permanent lesion can dramatically increase the basal synaptic transmission. This effect may be associated with an increase in the neurotransmitter content of the pre-synaptic neurons. This hypothesis could provide a new insight into the relationship between IT and synaptic efficacy. Synapse 70:351-360, 2016. © 2016 Wiley Periodicals, Inc.


Assuntos
Potenciais Pós-Sinápticos Excitadores , Infarto da Artéria Cerebral Média/fisiopatologia , Traumatismo por Reperfusão/fisiopatologia , Sinapses/fisiologia , Animais , Circulação Cerebrovascular , Estimulação Encefálica Profunda , Potenciação de Longa Duração , Masculino , Ratos , Ratos Sprague-Dawley
18.
Brain Res Bull ; 124: 95-102, 2016 06.
Artigo em Inglês | MEDLINE | ID: mdl-27066884

RESUMO

Fingolimod (FTY720) is a known sphingosine-1-phosphate (S1P) receptor agonist. Several studies have shown the therapeutic efficacy of FTY720 in neurodegenerative disorders. However, the neuroprotective mechanisms in brain ischemia have not been adequately studied. Therefore, the present study aimed to investigate the effects of FTY720 on the impairment of learning and memory and hippocampal synaptic plasticity induced by middle cerebral artery occlusion (MCAO) in ischemic brain injury. Twenty eight male rats were randomly divided into four groups of control (n=7), sham (n=8), ischemic-reperfusion+vehicle (I/R+V; n=7), and I/R+FTY720 (n=6). After 1h of the occlusion of artery, the filament was gently withdrawn to allow reperfusion for the next 7 days. The animals first received a dose of FTY720 (0.5mg/Kg) or its vehicle (intra-peritoneal) twenty-four hours before surgery in I/R+FTY720 and I/R+V groups, respectively. The administration of FTY720 or its vehicle continued every other day. The passive avoidance test and field potential recording were used for evaluation of learning, memory and synaptic plasticity. The brain infarct volume was measured by triphenyltetrazolim hydrochloride (TTC) staining. MCAO caused infarct damage in the rat's brain tissue. The administration of FTY720 significantly reduced the size of the lesion, improved the memory impairment of MCAO rats, and increased the STL time. In addition, the field potential recording demonstrated a marked reduction in induction of long-term potentiation of MCAO animals. However, administration of FTY720 recovers the magnitude of the LTP without any effects on presynaptic plasticity and neurotransmitter release probability. The results of this study demonstrated that MCAO in rats impairs the retention of passive avoidance tasks and multiple injection of FTY720 improved the memory performance after MCAO by LTP induction via post-synaptic mechanisms.


Assuntos
Cloridrato de Fingolimode/uso terapêutico , Hipocalcina/efeitos dos fármacos , Imunossupressores/uso terapêutico , Infarto da Artéria Cerebral Média/patologia , Transtornos da Memória/etiologia , Plasticidade Neuronal/efeitos dos fármacos , Animais , Aprendizagem da Esquiva/efeitos dos fármacos , Infarto Encefálico/tratamento farmacológico , Infarto Encefálico/etiologia , Modelos Animais de Doenças , Infarto da Artéria Cerebral Média/complicações , Potenciação de Longa Duração/efeitos dos fármacos , Linfócitos/efeitos dos fármacos , Masculino , Transtornos da Memória/tratamento farmacológico , Exame Neurológico , Neutrófilos/efeitos dos fármacos , Ratos , Ratos Sprague-Dawley , Reperfusão , Fatores de Tempo
19.
Oman Med J ; 31(1): 22-8, 2016 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-26816565

RESUMO

OBJECTIVES: The effect of dietary protein intake on bone mineral density (BMD) has not been explained in patients with spinal cord injury (SCI). In this study, we looked at the relationship between BMD and higher protein intake in patients with SCI while controlling for possible confounders. METHODS: Patients with SCI, who were referred to the Brain and Spinal Cord Injury Research Center between November 2010 and April 2012, were included in the study. In total, the dietary intakes of 103 patients were assessed by 24-hour dietary recall interviews. We used dual-energy X-ray absorptiometry to measure BMD in the femoral neck, trochanter, intertrochanteric zone, hip, and lumbar vertebras. RESULTS: Eighty-six men and 17 women participated in this study. Protein intake was negatively associated with the BMD of lumbar vertebrae (p = 0.001, r = -0.37 for T-score and p = 0.030, r = -0.24 for Z-score). The BMD of lumbar vertebrae were negatively associated with intake of tryptophan, isoleucine, lysine, cysteine, and tyrosine (p = 0.007, 0.005, 0.009, 0.008, and 0.008 for T-score, respectively). Higher intakes of threonine, leucine, methionine, phenylalanine, valine, and histidine were related to a lower BMD of lumbar vertebrae (p = 0.006, 0.010, 0.009, 0.010, 0.009, and 0.008 respectively for T-scores). CONCLUSIONS: We found that high protein intake led to a lower BMD of lumbar vertebrae in patients with SCI after controlling for confounders including demographic and injury-related characteristics and calcium intake. No relationship between higher amino acids intake and BMD of the femur and hip was detected. Intake of alanine, arginine, and aspartic acid were not related to BMD.

20.
Pak J Biol Sci ; 19(5): 227-232, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-29023027

RESUMO

BACKGROUND AND OBJECTIVE: Molecular mechanisms of most anti-obesity drugs are remained to be clear. MicroRNAs that are noncoding RNA molecules supposed to regulate biological processes concomitant to obesity and have attracted a lot of attention to themselves. The miR-27a and miR-143 expression levels in obese and non-obese rats during weight changes and L-carnitine (LC) effects on them was investigated in this study. MATERIALS AND METHODS: In the present study 12 male Wistar rats were randomly divided into normal fat diet and high fat diet groups to develop obesity. After 8 weeks rats were weighted and half of diet induced obese rats were randomly selected to receive 200 mg LC kg -1 b.wt. for 4 weeks. At the end epididymal fat was isolated to investigate expression level of microRNAs by real-time PCR. RESULTS: After 12 weeks, high fat diet in comparison with normal fat diet mediated significant decrease and increase in expression levels of miR-27a and miR-143 , respectively. These changes were modified in groups, which had received LC in a 4 weeks period. Furthermore, rats in this group gained less weight. CONCLUSION: Findings of this study suggest that the changes of microRNAs expression probably play a role in pathogenesis of obesity, might be modulated by means of dietary agents and supplements and modify weight gain trend.


Assuntos
Adipogenia/efeitos dos fármacos , Tecido Adiposo/efeitos dos fármacos , Fármacos Antiobesidade/farmacologia , Carnitina/farmacologia , MicroRNAs/genética , Obesidade/tratamento farmacológico , Perda de Peso/efeitos dos fármacos , Adipogenia/genética , Tecido Adiposo/metabolismo , Animais , Dieta Hiperlipídica , Modelos Animais de Doenças , Regulação da Expressão Gênica/efeitos dos fármacos , Masculino , MicroRNAs/metabolismo , Obesidade/genética , Obesidade/metabolismo , Obesidade/fisiopatologia , Ratos Wistar , Fatores de Tempo
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