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1.
Infect Genet Evol ; 84: 104378, 2020 May 26.
Artigo em Inglês | MEDLINE | ID: mdl-32464310

RESUMO

The Candida parapsilosis complex has been associated with highly refractory infections mainly due to the presence of biofilms. High glucose levels enable the development of this virulence factor which can aggravate the clinical condition of patients with diabetes mellitus, those using parenteral nutrition, with invasive medical device, including others. Combined antifungal therapy, such as azole and cyclooxygenase inhibitors, may be an alternative in such infections since they modulate prostaglandin production favoring the adhesion and development of biofilms. Thus, the present study aimed to evaluate the influence of glucose supplementation in the formation and detection of Candida parapsilosis complex biofilms and to treat them using fluconazole and a cyclooxygenase inhibitor in combination. Protein spectra evaluation allowed the differentiation between species from the complex (score > 2) in our studies. All isolates were able to form active biofilms at different glucose concentrations. In addition, a significant reduction in biofilm formation was observed when fluconazole and acetylsalicylic acid were combined. The ultrastructural analysis presented typical biofilm characteristics by species from the complex. These data support new combined therapies for the treatment of fungal infections, especially with those which are resistant and therapeutic failure is associated with virulence factors.

2.
PLoS Negl Trop Dis ; 14(3): e0008151, 2020 03.
Artigo em Inglês | MEDLINE | ID: mdl-32226021

RESUMO

Sporothrix chilensis is a mild-pathogenical specie of Sporothrix pallida complex, until now, known as restrict to Chile. Herein, we describe the first clinical isolates identified as S. chilensis in Brazil, preserved in the URM Culture Collection, by polyphasic taxonomy, and their respective antifungal profile of this emergent fungus.


Assuntos
Sporothrix/classificação , Sporothrix/isolamento & purificação , Esporotricose/microbiologia , Antifúngicos/farmacologia , Brasil , Humanos , Testes de Sensibilidade Microbiana , Técnicas de Tipagem Micológica , Análise de Sequência de DNA , Sporothrix/genética , Sporothrix/fisiologia , Tubulina (Proteína)/genética
3.
Braz J Microbiol ; 51(2): 647-655, 2020 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-32141030

RESUMO

The objective of this study was to evaluate the effects of nanoparticles (nanospheres and nanocapsules) of the promising antifungal 2-amino-thiophene (6CN10) and 6CN10 complexed with 2-hydroxypropyl-ß-cyclodextrin (6CN10:HP-ß-CD) in vitro and compared with free drug against Candida and Cryptococcus, using a microdilution method to measure susceptibility. The Candida and Cryptococcus clinical strains were identified using phenotypic methods and matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF). To measure in vitro antifungal susceptibility, we used microdilution trials. Serial drug or nanoparticle dilutions were prepared according to the CLSI M27-A3 guidelines. Anti-biofilm activity was verified for Cryptococcus neoformans. All Candida isolates were sensitive to the free drug (MIC = 41.66-333.33 µg/mL) and were able to grow even at the higher concentration tested for all 6CN10 nanoparticles. However, the Cryptococcus neoformans strains presented MIC values of 0.32-83.33 µg/mL for 6CN10 nanoparticles, and MIC values of 0.1-0.2 µg/mL for 6CN10:HP-ß-CD nanoparticles, i.e., 3333 times more active than the free drug (MIC values 166.66-333.33 µg/mL), and presenting activity greater than that of the reference drug amphotericin B (MIC = 0.5-0.125 µg/mL). 6CN10:HP-ß-CD nanosphere also showed high anti-biofilm potential. The in vitro study showed that the nanoparticles allowed better drug efficiency against Cryptococcus than did the free drug. These results suggest that 6CN10-loaded nanoparticles may become a future alternative for cryptococcosis and candidiasis therapy. In vivo experiments are essential prior to clinical use.

4.
Sci Total Environ ; 703: 134779, 2020 Feb 10.
Artigo em Inglês | MEDLINE | ID: mdl-31710846

RESUMO

The search for natural substances such as plant extracts with antimicrobial properties has considerably increased, given that biofilms constitute a barrier against antifungal therapy, where these can be formed on any surface, such as acrylic resin prosthesis. The objective of this study was to identify the chemical composition of the Persea americana Mill. leaf ethanol extract (EEFPa) using the UPLC-QTOF-MS/MS technique, to verify its antifungal activity through a sensitivity test according to the conditions described in the documents in M27-A3 (CLSI, 2008) and M60 (CLSI, 2017), to induce biofilm formation in acrylic resin discs and quantify their formation using tetrazolium salt reduction (MTT), as well as to treat these with the extract and fluconazole. Ten of the twelve compounds present in the extract were identified. In the sensitivity test the lowest minimum inhibitory concentration observed was 512 µg/mL, while fluconazole concentrations ranged from 64 to 1 µg/mL. During biofilm induction, all the isolates were able to form biofilms within 48 h. During biofilm treatment, the extract was less effective at biofilm reduction than Fluconazole. The EEFPa showed significant antifungal activity against some of the strains in this study, however the extract showed lower effect when compared to fluconazole against the biofilm formation.


Assuntos
Persea , Resinas Acrílicas , Antifúngicos , Biofilmes , Produtos Biológicos , Candida , Testes de Sensibilidade Microbiana , Folhas de Planta , Espectrometria de Massas em Tandem , Árvores
5.
Rev. iberoam. micol ; 36(4): 181-185, oct.-dic. 2019. tab, graf
Artigo em Inglês | IBECS | ID: ibc-191413

RESUMO

Background: Candidemia is a life-threatening fungal infection characterized by the presence of Candida in the blood. Aims: To describe the clinical-epidemiological features and main risk factors among patients with candidemia admitted to Intensive Care Unit. Methods: A cross-sectional, retrospective and observational study was performed between January 2015 and July 2016. Laboratory reports and medical records from ICU patients admitted to a public hospital in northeastern Brazil were analyzed. Results: There were 1573 admissions and 67 of them were positive for candidemia. The majority of patients were male (53.3%) and remained at the hospital for more than seven days (86.6%). Non-C. albicans Candida infections (60%) were predominant. Broad-spectrum antibiotic therapy was prescribed in 98.4% of the cases. The most frequent underlying diseases were sepsis (73.3%), presence of solid tumors (15%), respiratory condition (60%), urinary tract disease (56.6%) and gastrointestinal tract diseases (23.3%). Surgeries were carried out on 43% of the patients, consisting of 23.3% abdominal surgeries, with a mortality rate of 92.8%. Risk factors were venous central access (93.3%), mechanical ventilation (81.6%), nasoenteral tube (83.3%), nasogastric tube (25%), indwelling bladder catheter (88.3%), diabetes mellitus (55%) and tracheostomy (36.6%). Statistical analysis correlated the use of indwelling bladder catheter with a higher mortality rate (r=0.07412, p=0.0353). Conclusions: The current study reveals the high case fatality rates among critically ill patients suffering from candidemia admitted to ICU. Herein, we highlight the importance of identifying non-C. albicans Candida species and reinforce the idea of carrying out epidemiological surveillances and antifungal susceptibility tests


Antecedentes: La candidemia es una infección potencialmente fatal caracterizada por la presencia de Candida en la sangre. Objetivos: Describir las características clínico-epidemiológicas y los principales factores de riesgo en pacientes con candidemia ingresados en la unidad de cuidados intensivos (UCI). Métodos: Entre enero de 2015 y julio de 2016 se llevó a cabo un estudio transversal, retrospectivo y observacional en el que se analizaron los registros médicos e informes de laboratorio de pacientes de la UCI de un hospital público del noreste de Brasil. Resultados: Entre las 1.573 admisiones registradas hubo 67 diagnósticos de candidemia. La mayoría de los pacientes fueron del sexo masculino (53,3%) y la permanencia en el hospital fue superior a siete días (86,6%). Las infecciones por especies de Candida no-C. albicans fueron el 60% de los casos. En el 98,4% de los casos se prescribió antibioterapia de amplio espectro. Las enfermedades de base más frecuentes fueron la sepsis (73,3%), la presencia de tumores sólidos (15%), las enfermedades respiratorias (60%), la enfermedad del tracto urinario (56,6%) y las enfermedades del tracto gastrointestinal (23,3%). Se realizaron cirugías en el 43% de los pacientes, siendo el 23,3% cirugías abdominales, con una tasa de mortalidad del 92,8%. Los factores de riesgo fueron la existencia de un acceso venoso central (93,3%), ventilación mecánica (81,6%), sonda nasoenteral (83,3%), sonda nasogástrica (25%), catéter vesical permanente (88,3%), diabetes mellitus (55%) y traqueostomía (36,6%). Los análisis estadísticos correlacionaron el uso del catéter urinario permanente con una mayor mortalidad (r=0,07412; p=0,0353). Conclusiones: Este estudio muestra las elevadas tasas de letalidad asociadas a pacientes con candidemia ingresados en la UCI. Destacamos la creciente importancia de identificar las especies de Candida diferentes de C. albicans, y reforzamos la idea de llevar a cabo una vigilancia epidemiológica y de realizar pruebas de sensibilidad a los antifúngicos


Assuntos
Humanos , Masculino , Feminino , Pessoa de Meia-Idade , Idoso , Idoso de 80 Anos ou mais , Candida/isolamento & purificação , Candidíase/complicações , Candidemia/epidemiologia , Infecção Hospitalar/epidemiologia , Brasil/epidemiologia , Fatores de Risco , Estado Terminal/epidemiologia , Unidades de Terapia Intensiva/estatística & dados numéricos
6.
Rev Iberoam Micol ; 36(4): 181-185, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31759839

RESUMO

BACKGROUND: Candidemia is a life-threatening fungal infection characterized by the presence of Candida in the blood. AIMS: To describe the clinical-epidemiological features and main risk factors among patients with candidemia admitted to Intensive Care Unit. METHODS: A cross-sectional, retrospective and observational study was performed between January 2015 and July 2016. Laboratory reports and medical records from ICU patients admitted to a public hospital in northeastern Brazil were analyzed. RESULTS: There were 1573 admissions and 67 of them were positive for candidemia. The majority of patients were male (53.3%) and remained at the hospital for more than seven days (86.6%). Non-C. albicansCandida infections (60%) were predominant. Broad-spectrum antibiotic therapy was prescribed in 98.4% of the cases. The most frequent underlying diseases were sepsis (73.3%), presence of solid tumors (15%), respiratory condition (60%), urinary tract disease (56.6%) and gastrointestinal tract diseases (23.3%). Surgeries were carried out on 43% of the patients, consisting of 23.3% abdominal surgeries, with a mortality rate of 92.8%. Risk factors were venous central access (93.3%), mechanical ventilation (81.6%), nasoenteral tube (83.3%), nasogastric tube (25%), indwelling bladder catheter (88.3%), diabetes mellitus (55%) and tracheostomy (36.6%). Statistical analysis correlated the use of indwelling bladder catheter with a higher mortality rate (r=0.07412, p=0.0353). CONCLUSIONS: The current study reveals the high case fatality rates among critically ill patients suffering from candidemia admitted to ICU. Herein, we highlight the importance of identifying non-C. albicansCandida species and reinforce the idea of carrying out epidemiological surveillances and antifungal susceptibility tests.

7.
Mycopathologia ; 184(4): 547-549, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31230198

RESUMO

The article describes an atypical case of cat-transmitted ocular sporotrichosis due Sporothrix brasiliensis in a young patient. This report contributes significantly to knowledge of sporotrichosis as an important neglected tropical disease. In addition, this condition is an important public health problem in ascending incidence.


Assuntos
Doenças do Gato/transmissão , Oftalmopatias/diagnóstico , Oftalmopatias/patologia , Sporothrix/isolamento & purificação , Esporotricose/diagnóstico , Esporotricose/patologia , Zoonoses/transmissão , Adulto , Animais , Gatos , Humanos , Masculino , Técnicas Microbiológicas
9.
PLoS One ; 12(11): e0188020, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-29145445

RESUMO

The majority of microorganisms present a community lifestyle, establishing biofilm ecosystems. However, little is known about its formation in emergent Candida species involved in catheter-related infections. Thus, various techniques may be used in the biofilm detection to elucidate structure and clinical impact. In this context, we report the ability of emergent Candida species (Candida haemulonii, C. lusitaniae, C. pelliculosa, C.guilliermondii, C. famata and C. ciferrii) on developing well structured biofilms with cell viability and architecture, using optical coherence tomography (OCT). This new approach was compared with XTT analyses and Scanning Electron Microscopy (SEM). A positive correlation between oxidative activity (XTT) and OCT results (r = 0.8752, p < 0.0001) was observed. SEM images demonstrated cells attachment, multilayer and morphologic characteristics of the biofilm structure. C. lusitaniae was the emergent species which revealed the highest scattering extension length and oxidative metabolism when evaluated by OCT and XTT methods, respectively. Herein, information on C. ciferri biofilm structure were presented for the first time. The OCT results are independently among Candida strains and no species-specific pattern was observed. Our findings strongly contribute for clinical management based on the knowledge of pathogenicity mechanisms involving emergent yeasts.


Assuntos
Biofilmes , Candida/fisiologia , Tomografia de Coerência Óptica/métodos , Candida/classificação , Microscopia Eletrônica de Varredura , Especificidade da Espécie
10.
Braz. j. microbiol ; 48(2): 208-210, April.-June 2017. graf
Artigo em Inglês | LILACS | ID: biblio-839387

RESUMO

Abstract This communication reports the second known case of oral phaeohyphomycosis in a patient with squamocellular carcinoma of the lip. The patient, an 82-year-old black woman, a former smoker (for more than 30 years), suffering from an ulcerous vegetative lesion in the middle third of the lower lip for approximately 12 months. The result of the histopathological analysis indicated carcinoma, with well-differentiated keratinized squamous cells and the presence of septate mycelial filaments. In the direct mycological examination, thick and dematiaceous septate mycelial filaments were observed. After the resection surgery, the patient did not need to use an antifungal drug to treat the phaeohyphomycosis, and no follow-up radiotherapy was needed to treat the squamocellular carcinoma. We stress that the presence of the squamocellular lesion of the lip was a possible contributing factor to the infection.


Assuntos
Humanos , Feminino , Idoso de 80 Anos ou mais , Neoplasias Labiais/complicações , Carcinoma de Células Escamosas/complicações , Feoifomicose/diagnóstico , Feoifomicose/patologia , Lábio/patologia , Brasil , Neoplasias Labiais/cirurgia , Carcinoma de Células Escamosas/cirurgia , Técnicas Microbiológicas , Resultado do Tratamento , Feoifomicose/cirurgia , Histocitoquímica , Lábio/cirurgia , Microscopia
11.
Chem Biol Interact ; 272: 172-181, 2017 Jun 25.
Artigo em Inglês | MEDLINE | ID: mdl-28479098

RESUMO

In the present work, twelve N-substituted 2-(5-nitro-thiophene)-thiosemicarbazones derivatives (L1-12) were synthesized, characterized and their in vitro cytotoxic and antifungal activities were evaluated against Candida sp. and Cryptococcus neoformans. The probable mechanisms of action have been investigated by sorbitol and ergosterol assays. Additionally, ultrastructural study by Scanning Electron Microscopy was performed with the L10 compound. All compounds were obtained in good yield and their chemical structures were characterized on basis of their physico-chemical and Nuclear Magnetic Resonance - NMR, Spectrophotometric Absorption in the Infrared - IR and High-resolution Mass Spectrometry - HRMS data. The results showed that all strains were more sensitive to the compound L10 except Candida tropicalis URM 6551. On the other hand, the cytotoxicity assay by incorporation of tritiated thymidine showed moderate cytotoxic activity on L8 of the 50 µg/mLat which had the best MIC-cytotoxicity relationship. Concerning the study of the possible mechanism of action, the compounds were not able to bind to ergosterol in the membrane, do not act by inhibiting the synthesis of fungal cell wall (sorbitol assay). However, the Scanning Electron Microscopy - SEM analysis shows significant morphological changes in shape, size, number of cells and hyphae, and cell wall indicating a possible mechanism of action by inhibition of enzymes related to the ergosterol biosynthesis pathway. Our results demonstrate that N-substituted 2-(5-nitro-thiophene)-thiosemicarbazones derivatives are potential antifungal agents with activity associated with inhibition of enzymes related to biosynthesis of ergosterol.


Assuntos
Antifúngicos/síntese química , Tiossemicarbazonas/química , Animais , Antifúngicos/química , Antifúngicos/toxicidade , Candida/efeitos dos fármacos , Sobrevivência Celular/efeitos dos fármacos , Espectroscopia de Ressonância Magnética , Espectrometria de Massas , Camundongos , Camundongos Endogâmicos BALB C , Testes de Sensibilidade Microbiana , Microscopia Eletrônica de Varredura , Baço/citologia , Baço/efeitos dos fármacos , Baço/metabolismo , Tiossemicarbazonas/síntese química , Tiossemicarbazonas/toxicidade
12.
Braz J Microbiol ; 48(2): 208-210, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-28057425

RESUMO

This communication reports the second known case of oral phaeohyphomycosis in a patient with squamocellular carcinoma of the lip. The patient, an 82-year-old black woman, a former smoker (for more than 30 years), suffering from an ulcerous vegetative lesion in the middle third of the lower lip for approximately 12 months. The result of the histopathological analysis indicated carcinoma, with well-differentiated keratinized squamous cells and the presence of septate mycelial filaments. In the direct mycological examination, thick and dematiaceous septate mycelial filaments were observed. After the resection surgery, the patient did not need to use an antifungal drug to treat the phaeohyphomycosis, and no follow-up radiotherapy was needed to treat the squamocellular carcinoma. We stress that the presence of the squamocellular lesion of the lip was a possible contributing factor to the infection.


Assuntos
Carcinoma de Células Escamosas/complicações , Neoplasias Labiais/complicações , Lábio/patologia , Feoifomicose/diagnóstico , Feoifomicose/patologia , Idoso de 80 Anos ou mais , Brasil , Carcinoma de Células Escamosas/cirurgia , Feminino , Histocitoquímica , Humanos , Lábio/cirurgia , Neoplasias Labiais/cirurgia , Técnicas Microbiológicas , Microscopia , Feoifomicose/cirurgia , Resultado do Tratamento
13.
Springerplus ; 5: 468, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-27119072

RESUMO

This work aimed to evaluate the antifungal activity of piroctone olamine in the treatment of intra-abdominal candidiasis in an experimental model using Swiss mice. The mice (n = 6) were infected by intraperitoneal injection of 0.2 ml of C. albicans (10(7)cells/ml in saline). The animals were observed daily for clinical signs and mortality for 14 days. The treatment with piroctone olamine (0.5 mg/kg) was performed 72 h after infection by intraperitoneal administration. For comparison, a group of animals (n = 6) was treated with amphotericin B (0.5 mg/kg). The mycological diagnosis was made by collecting the liver, spleen and kidneys. Data regarding the fungal growth and mortality were analyzed statistically by Student's t test and analysis of variance (ANOVA), with level of significance set at P < 0.05. The difference in fungal growth scoring between the control group and the treatment groups (piroctone olamine and amphotericin B) was statistically significant (P < 0.05). The difference in fungal growth scoring between the treatment groups (piroctone olamine and amphotericin B) was not statistically significant (P < 0.05).

14.
Braz. j. infect. dis ; 19(6): 660-663, Nov.-Dec. 2015. tab, graf
Artigo em Inglês | LILACS | ID: lil-769617

RESUMO

ABSTRACT Infections caused by emerging Cryptococcus non-neoformans species are being reported with increasingly frequency. Here, we present a case of fungaemia byCryptococcus laurentii in a woman receiving aggressive immunosuppressive therapy for cervical neoplasia. Three venous blood samples were aseptically collected on consecutive days and C. laurentiiwas isolated and identified through phenotypic and molecular methods. After central venous catheter removal and appropriate antifungal therapy, the patient showed significant improvement and blood culture became negative. Thus, patients following immunosuppressive therapies and using invasive medical devices are at risk of C. laurentii blood infections.


Assuntos
Adulto , Feminino , Humanos , Neoplasia Intraepitelial Cervical/complicações , Criptococose/microbiologia , Fungemia/microbiologia , Hospedeiro Imunocomprometido/imunologia , Neoplasias do Colo do Útero/complicações , Neoplasia Intraepitelial Cervical/microbiologia , Criptococose/diagnóstico , Criptococose/imunologia , Cryptococcus/genética , Cryptococcus/isolamento & purificação , Fungemia/diagnóstico , Fungemia/imunologia , Neoplasias do Colo do Útero/microbiologia
15.
Braz J Infect Dis ; 19(6): 660-3, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-26361842

RESUMO

Infections caused by emerging Cryptococcus non-neoformans species are being reported with increasingly frequency. Here, we present a case of fungaemia by Cryptococcus laurentii in a woman receiving aggressive immunosuppressive therapy for cervical neoplasia. Three venous blood samples were aseptically collected on consecutive days and C. laurentii was isolated and identified through phenotypic and molecular methods. After central venous catheter removal and appropriate antifungal therapy, the patient showed significant improvement and blood culture became negative. Thus, patients following immunosuppressive therapies and using invasive medical devices are at risk of C. laurentii blood infections.


Assuntos
Neoplasia Intraepitelial Cervical/complicações , Criptococose/microbiologia , Fungemia/microbiologia , Hospedeiro Imunocomprometido/imunologia , Neoplasias do Colo do Útero/complicações , Adulto , Neoplasia Intraepitelial Cervical/microbiologia , Criptococose/diagnóstico , Criptococose/imunologia , Cryptococcus/genética , Cryptococcus/isolamento & purificação , Feminino , Fungemia/diagnóstico , Fungemia/imunologia , Humanos , Neoplasias do Colo do Útero/microbiologia
18.
Biosci. j. (Online) ; 31(2): 451-459, mar./abr. 2015.
Artigo em Português | LILACS | ID: biblio-964078

RESUMO

Considerando as perdas causadas em pós-colheita pela antracnose no pimentão e à ineficácia das medidas de controle atualmente utilizadas, este trabalho teve por objetivo estudar a potencialidade antagônica in vitro e in vivo de quinze isolados de leveduras à Colletotrichum sp., agente causal da antracnose em pimentão. Foi calculada a porcentagem de inibição do crescimento do fungo dos tratamentos em relação à testemunha nos testes in vitro e determinado o tamanho da área lesionada em frutos através da mensuração do comprimento da lesão em dois sentidos diametricamente opostos nos testes in vivo. As leveduras que obtiveram o melhor resultado no controle da antracnose foram identificadas através de características macroscópicas, microscópicas, fisiológicas, bioquímicas e por taxonomia molecular, sendo os isolados de levedura 13E e 13A1, os que obtiveram as melhores respostas no controle do fitopatógeno tanto in vitro quanto in vivo, identificados como pertencentes à espécie Rhodotorula glutinis.


Considering the losses caused by post-harvest anthracnose in sweet pepper chili and ineffectiveness of control measures currently used, this study had the objective of evaluate the antagonistic potential in vitro and in vivo of fifteen isolates of yeast to Colletotrichum sp., the causal agent of anthracnose on pepper. By calculating the percentage growth inhibition of the fungus treatment compared to the control tests in vitro and determining the size of lesions via the measurement of lesion length in both directions diametrically affixed in vivo tests. Yeasts who obtained the best result in controlling anthracnose were identified by characteristic macroscopic, microscopic, physiological, biochemical and molecular taxonomy. In this study, the yeast isolates 13E and 13A1, which obtained the best results in controlling the pathogen both in vitro and in vivo of the species Rhodotorula glutinis.


Assuntos
Rhodotorula , Leveduras , Capsicum , Controle Biológico de Vetores , Colletotrichum
19.
Molecules ; 20(3): 3776-82, 2015 Feb 25.
Artigo em Inglês | MEDLINE | ID: mdl-25723851

RESUMO

Cryptococcus neoformans var. grubii is considered to be the major cause of cryptococcosis in immunosuppressed patients. Understanding cell wall glycoproteins using lectins is of medical interest and can contribute to specific therapy. The aim of this study was to evaluate the carbohydrates on the cell wall of Cryptococcus neoformans var. grubii clinical isolates, using a fluorescein isothiocyanate-lectin binding protocol. Thirty yeast strains stocked in the culture collection were cultivated for 2 days at 30 °C with shaking. Cells were obtained by centrifugation, washed in phosphate-buffered saline, and a suspension of 107 cells/mL was obtained. To determine the binding profile of lectins, concanavalin A (Con A), wheat germ agglutinin (WGA), Ulex europaeus agglutinin I (UEA-I), and peanut agglutinin (PNA) conjugated to fluorescein were used. All the tested clinical isolates of Cryptococcus neoformans var. grubii were intensely stained by WGA, moderately stained by Con A, and weakly stained by PNA and UEA-I. Thus, Cryptococcus can be detected in clinical specimens such as blood and cerebrospinal fluid using the fluorescent lectin WGA, which may be considered as an option for detection in cases of suspected cryptococcosis with low laboratory sensitivity. Future applications may be developed using this basic tool.


Assuntos
Carboidratos , Parede Celular/metabolismo , Concanavalina A/metabolismo , Criptococose/metabolismo , Cryptococcus neoformans/isolamento & purificação , Cryptococcus neoformans/metabolismo , Lectinas/metabolismo , Criptococose/microbiologia , Criptococose/patologia , Humanos
20.
Mycopathologia ; 179(3-4): 225-9, 2015 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-25431088

RESUMO

The aim of this study was to assess the efficacy of topical application of a liposomal formulation of itraconazole for the treatment of experimental keratitis with endophthalmitis caused by Aspergillus flavus. The liposomes were obtained by the lipid film hydration method followed by sonication. Adult female Wistar rats (weighing 200-220 g) were immunosuppressed by intraperitoneal injection of 150 mg/kg of cyclophosphamide 3 days before infection by exposure to the fungus A. flavus (10(7) spores/ml). Forty-eight hours later, the animals were treated with the liposomal formulation. For comparison, one group of animals (n = 6) was treated with the same drug not encapsulated. At the end of the experiment, the animals were evaluated for clinical signs and number of colony forming units (CFU/g), along with direct microscopic examination. The results indicated that the liposomal formulation of itraconazole has better antifungal activity than the unencapsulated drug in the treatment of fungal keratitis with endophthalmitis caused experimentally by A. flavus in Wistar rats.


Assuntos
Antifúngicos/administração & dosagem , Aspergilose/tratamento farmacológico , Aspergillus flavus/efeitos dos fármacos , Química Farmacêutica , Endoftalmite/tratamento farmacológico , Itraconazol/administração & dosagem , Ceratite/tratamento farmacológico , Animais , Antifúngicos/química , Aspergilose/microbiologia , Aspergillus flavus/fisiologia , Endoftalmite/microbiologia , Feminino , Humanos , Itraconazol/química , Ceratite/microbiologia , Lipossomos/química , Ratos , Ratos Wistar
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