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1.
Bioresour Technol ; : 127307, 2022 May 12.
Artigo em Inglês | MEDLINE | ID: mdl-35569712

RESUMO

This work studied the impacts of key granule properties on the granular reactor performing partial nitritation/anammox from the modeling perspective. The results could guide not only future reliable modeling but also practical startup/operation of the reactor. To achieve high total nitrogen (TN) removal whilst avoiding significant N2O production, inoculated granules should be big and anammox-enriched. The optimum boundary layer thickness for maximum TN removal increased with the decreasing diffusivity of soluble components in the granule structure. Even though a thick boundary layer could protect anammox bacteria from elevated dissolved oxygen (DO) (e.g., 0.5 g-O2/m3) and obtain high TN removal (>90.0%) and low N2O production (<1.8%), even complete removal of the boundary layer would fail to provide sufficient substrate for anammox and therefore couldn't increase TN removal to 90.0% and decrease N2O production to <2.4% at insufficient DO (e.g., 0.3 g-O2/m3 in the presence of lifted influent NH4+ concentration).

2.
Sci Total Environ ; 835: 155411, 2022 Apr 29.
Artigo em Inglês | MEDLINE | ID: mdl-35490813

RESUMO

This work aimed to reflect the advancements in water-related science, technology, and policy and shed light on future research opportunities related to water through a systematic overview of Water Research articles published in the first 21.5 years of the 21st century. Specific bibliometric analyses were performed to i) reveal the temporal and spatial trends of water-related research themes and ii) identify the underlying connections between research topics. The results showed that while top topics including wastewater (treatment), drinking water, adsorption, model, biofilm, and bioremediation remained constantly researched, there were clear shifts in topics over the years, leading to the identification of trending-up and emerging research topics. Compared to the first decade of the 21st century, the second decade not only experienced significant uptrends of disinfection by-products, anaerobic digestion, membrane bioreactor, advanced oxidation processes, and pharmaceuticals but also witnessed the emerging popularity of PFAS, anammox, micropollutants, emerging contaminants, desalination, waste activated sludge, microbial community, forward osmosis, antibiotic resistance genes, resource recovery, and transformation products. On top of the temporal evolution, distinct spatial evolution existed in water-related research topics. Microplastics and Covid-19 causing global concerns were hot topics detected, while metagenomics and machine learning were two technical approaches emerging in recent years. These consistently popular, trending-up and emerging research topics would most likely attract continuous/increasing research input and therefore constitute a major part of the prospective water-related research publications.

3.
J Environ Manage ; 316: 115230, 2022 May 07.
Artigo em Inglês | MEDLINE | ID: mdl-35537269

RESUMO

Excess sludge management is a restrictive factor for the development of municipal wastewater treatment plants. The addition of metabolic uncouplers has been proven to be effective in sludge reduction. However, the long-term effect of metabolic uncoupler o-chlorophenol (oCP) on the biological wastewater treatment system operated in anaerobic-oxic mode is still unclear. To this end, two parallel reactors operated in anaerobic-oxic mode with and without 10 mg/L of oCP addition were investigated for 91 days. The results showed that 56.1 ± 2.3% of sludge reduction was achieved in the oCP-added system, and the nitrogen and phosphorus removal ability were negatively affected. Dosing oCP stimulated the formation of microbial products and increased the DNA concentration, but resulted in a decrease in the electronic transport activity of activated sludge. Microbial community analysis further demonstrated that a significant reduction of bacterial richness and diversity occurred after oCP dosing. However, after stopping oCP addition, the pollutant removal ability of activated sludge was gradually increased, but the sludge yield, as well as species richness and diversity, did not recover to the previous level. This study will provide insightful guidance on the long-term application of metabolic uncouplers in the activated sludge system.

4.
Bioact Mater ; 15: 29-43, 2022 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-35386360

RESUMO

Low back pain, mainly caused by intervertebral disc degeneration (IVDD), is a common health problem; however, current surgical treatments are less than satisfactory. Thus, it is essential to develop novel non-invasive surgical methods for IVDD treatment. Here, we describe a therapeutic strategy to inhibit IVDD by injecting hydrogels modified with the extracellular matrix of costal cartilage (ECM-Gels) that are loaded with cartilage endplate stem cells (CESCs). After loaded with CESCs overexpressing Sphk2 (Lenti-Sphk2-CESCs) and injected near the cartilage endplate (CEP) of rats in vivo, ECM-Gels produced Sphk2-engineered exosomes (Lenti-Sphk2-Exos). These exosomes penetrated the annulus fibrosus (AF) and transported Sphk2 into the nucleus pulposus cells (NPCs). Sphk2 activated the phosphatidylinositol 3-kinase (PI3K)/p-AKT pathway as well as the intracellular autophagy of NPCs, ultimately ameliorating IVDD. This study provides a novel and efficient non-invasive combinational strategy for IVDD treatment using injectable ECM-Gels loaded with CESCs that express Sphk2 with sustained release of functional exosomes.

5.
Water Res ; 218: 118432, 2022 Apr 08.
Artigo em Inglês | MEDLINE | ID: mdl-35472747

RESUMO

Polycyclic aromatic hydrocarbons (PAHs) are a group of common recalcitrant pollutant in industrial saline wastewater that raised significant concerns, whereas traditional activated sludge (AS) has limited tolerance to high salinity and PAHs toxicity, restricting its capacity to degrade PAHs. It is therefore urgent to develop a bioaugmented sludge (BS) system to aid in the effective degradation of these types of compounds under saline condition. In this study, a novel bioaugmentation strategy was developed by using halophilic Martelella sp. AD-3 for effectively augmented phenanthrene (PHE) degradation under 3% salinity. It was found that a 0.5∼1.5% (w/w) ratio of strain AD-3 to activated sludge was optimal for achieving high PHE degradation activity of the BS system with degradation rates reaching 2.2 mg⋅gVSS-1⋅h-1, nearly 25 times that of the AS system. Although 1-hydroxy-2-naphthoic acid (1H2N) was accumulated obviously, the mineralization of PHE was more complete in the BS system. Reads-based metagenomic coupled metatranscriptomic analysis revealed that the expression values of ndoB, encoding a dioxygenase associated with PHE ring-cleavage, was 5600-fold higher in the BS system than in the AS system. Metagenome assembly showed the members of the Corynebacterium and Alcaligenes genera were abundant in the strain AD-3 bioaugmented BS system with expression of 10.3±1.8% and 1.9±0.26%, respectively. Moreover, phdI and nahG accused for metabolism of 1H2N have been annotated in both above two genera. Degradation assays of intermediates of PHE confirmed that the activated sludge actually possessed considerable degradation capacity for downstream intermediates of PHE including 1H2N. The degradation capacity ratio of 1H2N to PHE was 87% in BS system, while it was 26% in strain AD-3. These results indicated that strain AD-3 contributed mainly in transforming PHE to 1H2N in BS system, while species in activated sludge utilized 1H2N as substrate to grow, thus establishing a syntrophic interaction with strain AD-3 and achieving the complete mineralization of PHE. Long-term continuous experiment confirmed a stable PHE removal efficiency of 93% and few 1H2N accumulation in BS SBR system. This study demonstrated an effective bioaugmented strategy for the bioremediation of saline wastewater containing PAHs.

6.
J Environ Manage ; 315: 115150, 2022 Apr 27.
Artigo em Inglês | MEDLINE | ID: mdl-35489188

RESUMO

Metal-laden solid wastes (e.g., waste graphite powders) have attracted great attention owing to their hazardous effects on the surrounding soil and water. Additionally, the metal-bearing impurities also hinder the reutilization of waste graphite powders. Thus, it is necessary to remove these inorganic impurities and figure out the removal mechanism of impurities in the purification process. In this study, an alkaline roasting-water washing-acid leaching (AWA) method was used to upgrade the waste graphite powders, and the migration behavior of diverse impurities has been qualitatively and quantitatively investigated. A graphite product with high impurity removal efficiencies is attained under optimal conditions. The removal of impurities mainly follows three routes: (1) V-, P-, and S-bearing impurities were complete removed (some formed soluble salts during alkaline roasting, and the remainder was dissolved in acid); (2) most Al-, K-, and Si-bearing impurities were removed by alkaline roasting, with the remainder was dissolved in the acid-leaching process; and (3) Fe-, Mg-, Ti-, Ca-, and Zn-bearing impurities were decomposed at high temperature and reacted with alkali to form hydroxides or oxides, which was subsequently dissolved in acid. In addition, the treatment of the generated wastewater is also discussed. The uncovered migration mechanisms of diverse impurities would guide the purification and reutilization process of other metal-bearing solid wastes efficiently.

7.
Water Res ; 217: 118426, 2022 Jun 15.
Artigo em Inglês | MEDLINE | ID: mdl-35427830

RESUMO

Microplastic fibers (MFs), as the most frequently detected microplastic shape in sewage sludge, have posed emerging concern for sludge treatment and disposal. However, the effect of MFs on antibiotic resistance genes (ARGs), especially extracellular ARGs (eARGs) during sludge treatment remains far from explicit. Therefore, this study investigated the potential impact of MFs on eARGs during sludge anaerobic digestion (AD), a commonly used sludge treatment method, through long-term operation. The qPCR results showed that both absolute and relative abundances of eARGs increased with the MFs exposure during sludge AD. The average absolute and relative abundances of eight tested eARGs in the AD reactor with the highest MFs dosage (170 items/gTS) were 1.70 and 2.15 times higher than those in the control AD reactor. The metagenomics results further comfirmed the increase of eARGs abundance during sludge anaerobic digestion after MFs exposure and the enhancement did not show significant selectivity. The identification of the potential hosts of eARGs suggested the host numbers of eARGs also increased with MFs exposure, which may suggest enhanced horizonal transformation as a result of increased eARGs. Further exploring the mechansims showed that the genes involved in type IV secretion system was upregulated after MFs exposure, suggesting the active release of eARGs was enhanced with MFs exposure. In contrast, the MFs may not affect the passive release of eARGs as its impact on cell membrance damage was insignificant. The enhanced eARGs in sludge AD process may further accelerate the transport of ARGs in environment, which should be considered during sludge treatment and disposal.

8.
Front Microbiol ; 13: 834208, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35401497

RESUMO

Although many reports have demonstrated that nanoparticles can have a negative effect on aquatic organisms, the toxic effects on symbiotic organisms remain poorly understood. The present study conducts ultrastructure, enzyme activity, and transcriptomics to assess the toxic effects to the Paramecium bursaria-Chlorella symbiotic system from exposure to copper nanoparticles (CuNPs) for 24 h. We found that in both the host and symbiotic algae, CuNP exposure induced high reactive oxygen species level, which leads to oxidative damage and energy metabolism disorder. Moreover, transmission electron micrographs (TEMs) showed that the symbiotic algae in the cytoplasm of P. bursaria were enveloped in the digestive vacuole and digested, and the level of acid phosphatase activity increased significantly within 24 h, which indicated that the stability of the symbiotic system was affected after CuNP exposure. We speculated that the increased energy demand in the host and symbiotic algae resulted from oxidative stress, precipitating the decrease of the photosynthetic products provided to the host, the digestion of the symbiont, and the destruction of the stable symbiotic relationship. The study provides the first insight into the mechanisms of nanoparticles' toxicity to the symbiotic relationship in the ecosystem, which may help to understand the environmental effects and toxicological mechanisms of nanoparticles.

9.
Microb Ecol ; 2022 Mar 22.
Artigo em Inglês | MEDLINE | ID: mdl-35316342

RESUMO

The single-celled eukaryote Euplotes aediculatus was chosen to test and compare the toxic effects of Cu and CuO nanoparticles (NPs). The antioxidant enzymatic activity, morphological changes, and functional groups on the membrane were determined using spectrophotometry, microscopy, and Fourier transform infrared spectroscopy after NPs treatment. The toxicity of the NPs to cells was dose-dependent, and the 24 h-LC50 values of the CuNPs and CuONPs were 0.46 µg/L and 1.24 × 103 µg/L, respectively. These NPs increased the activities of superoxide dismutase, glutathione peroxidase, and catalase and destroyed the cell structure; moreover, the CuNPs were more toxic than the CuONPs. In addition to the higher enzymatic activity, CuNPs also caused nucleoli disappearance, chromatin condensation, and mitochondrial and pellicle damage. The oxidization of the functional groups of the membrane (PO2 - , C-O-C, and δ(COH) of carbohydrates) also confirmed the severe damage caused by CuNPs. Our study showed that oxidative stress and organelle destruction played important roles in the toxic effects of these NPs on this protozoan. Compared with other aquatic organisms, E. aediculatus can be considered a potential indicator at the preliminary stage of environmental pollution.

10.
Environ Sci Technol ; 56(6): 3658-3668, 2022 03 15.
Artigo em Inglês | MEDLINE | ID: mdl-35254057

RESUMO

This study demonstrated that Fe3O4 simultaneously improves the total production and formation rate of medium-chain fatty acids (MCFAs) and long-chain alcohols (LCAs) from waste activated sludge (WAS) in anaerobic fermentation. Results revealed that when Fe3O4 increased from 0 to 5 g/L, the maximal MCFA and LCA production increased significantly, and the optimal fermentation time was also remarkably shortened from 24 to 9 days. Moreover, Fe3O4 also enhanced WAS degradation, and the corresponding degradation rate in the fermentation system increased from 43.86 to 72.38% with an increase in Fe3O4 from 0 to 5 g/L. Further analysis showed that Fe3O4 promoted the microbe activities of all the bioprocesses (including hydrolysis, acidogenesis, and chain elongation processes) involved in the MCFA and LCA production from WAS. Microbial community analysis indicated that Fe3O4 increased the abundances of key microbes involved in abovementioned bioprocesses correspondingly. Mechanistic investigations showed that Fe3O4 increased the conductivity of the fermented sludge, providing a better conductive environment for the anaerobic microbes. The redox cycle of Fe(II) and Fe(III) existed in the fermentation system with Fe3O4, which was likely to act as electron shuttles to conduct electron transfer (ET) from the electron donor to the acceptor, thus increasing ET efficiency. This study provides an effective method for enhancing the biotransformation of WAS into high-value products, potentially bringing economic benefits to WAS treatment.


Assuntos
Ácidos Graxos Voláteis , Esgotos , Anaerobiose , Biotransformação , Fermentação , Compostos Férricos , Concentração de Íons de Hidrogênio
11.
Sci Total Environ ; 828: 154404, 2022 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-35271918

RESUMO

Primary sludge (PS) and Waste activated sludge (WAS) as two main sludge streams in wastewater treatment plants are commonly anaerobically co-digested, which though may be differently affected by pretreatment. Previous work has found that calcium peroxide (CaO2) pretreatment effectively enhanced anaerobic digestion of WAS. However, the feasibilities of this strategy on PS anaerobic digestion and co-digestion of WAS and PS are still unclear. Herein, this work provided new insights into these systems. Biomethane potential test demonstrated that CaO2 pretreatment at 0.02-0.26 g/g-volatile suspended solids (VSS) promoted anaerobic digestion of PS. Then the feasibility of CaO2 pretreatment for improving anaerobic co-digestion of PS and WAS mixture was confirmed, with the highest improvement in methane production, VSS destruction and sludge reduction being approximately 37.4%, 38.9% and 19.9%, achieved at 0.14 g/g-VSS of CaO2. Process modelling analysis revealed that CaO2 pretreatment increased both degradable faction and actually degraded fraction in sludge mixture. The changes of sludge characteristics via pretreatment and key enzyme activity in sludge anaerobic co-digestion system demonstrated that increased CaO2 concentration resulted in increased soluble organics release from sludge mixture in the pretreatment stage and inhibited activity of coenzyme F420 responsible for methanogenesis. Further mechanism investigation disclosed that OH-, O2- and OH were main contribution factors, and the order of their contributions were OH- >O2- >OH. This work laid the theoretical foundation and provided guidance for the practical application of CaO2 pre-treatment technology.


Assuntos
Esgotos , Eliminação de Resíduos Líquidos , Anaerobiose , Reatores Biológicos , Digestão , Metano , Peróxidos , Eliminação de Resíduos Líquidos/métodos
12.
Sci Total Environ ; 828: 154428, 2022 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-35276160

RESUMO

The production of medium-chain fatty acids (MCFAs) is considered promising for carbon resource recovery from waste streams. However, a large quantity of external electron donors are often required, causing great cost and environmental impact. Therefore, in this study, a two-stage technology was developed to produce MCFAs from carbohydrate-rich wastewater without external electron donor addition, with the biofilm development and pH impact being explored. Stage I aimed at converting organics into ethanol and a yeast biofilm reactor is innovatively applied. The results showed that the yeast biofilm could quickly form on carriers with steady-state thickness reaching 50-200 µm. However, the attachment of yeast biofilm was weak at the initial stage so that the violent turbulence should be avoided during operation. The polyurethane foam was the most suitable for yeast biofilm development among the tested carriers, as evidenced by the highest ethanol production, accounting for 74.2% of soluble organics. The Nakaseomyces was the main fungal genus in the steady-state biofilm, while lactic acid bacteria were also developed, resulting in lactate and acetate production. In Stage II, the yeast biofilm reactor effluent was applied for MCFA production at different pH (5-8). However, the MCFA production selectivity was significantly affected by pH, with 65.2% at pH of 5 but decreasing substantially to 3.0% at pH of 8. Both the microbial and electron transfer efficiency analysis suggested that mildly acidic pH can promote the electron transfer from ethanol toward the chain elongation process instead of its excessive oxidation. Thus, if conditions of online extraction or microbial tolerance permit, a lower pH should be recommended for Stage II in the developed technology as well as other ethanol-based MCFA production process. This is a conceptual study that eliminated external electron donor addition in MCFAs production and provide a sustainable and reliable way in carbon resources recovery.


Assuntos
Saccharomyces cerevisiae , Águas Residuárias , Biofilmes , Carboidratos , Carbono , Elétrons , Etanol , Ácidos Graxos , Fermentação , Concentração de Íons de Hidrogênio
13.
Protist ; 173(2): 125867, 2022 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-35325828

RESUMO

The Class Nassophorea is not monophyletic with unsolved relationship of four orders, which calls for discussion to combine morphological features and molecular phylogeny. In the present study, the ultrastructure of Apocolpodidium etoschense in the order Colpodidiida is first studied. Comparisons between orders of Nassophorea were conducted and a discussion of systematics was performed based on a SSU rRNA gene-based phylogeny. The order Colpodidiida and Nassulida shared the following features: Two pairs of alveolocysts in the cortex, the presence of a ''B-cartwheel'' in the distal region of the kinetosome, the presence of cytostomal lamellae and subcytostomal lamellae in the cytopharyngeal basket, and spindle trichocysts with a simple tip. These similarities shape a core group of Nassophorea, which are morphologically and genetically different from the order Microthoracida. Consequently, Microthoracida should be regarded as an independent taxon rather than a member of Nassophorea. Within the core group of Nassophorea, Colpodidiida as an independent order is further validated by its delicate cytopharyngeal basket which lacks nematodesmal lamellae; while the non-monophyly of the order Nassulida might be explained by differentiation of the cartwheels in kinetosomes and the arrangement of kinetosomes with postciliary microtubules in the nassulid organelle 3 within its members.


Assuntos
Cilióforos , Filogenia
14.
Angew Chem Int Ed Engl ; 61(20): e202200753, 2022 May 09.
Artigo em Inglês | MEDLINE | ID: mdl-35238123

RESUMO

The growth of crystalline nanoparticles (NPs) generally involves three processes: nucleation, growth, and shape evolution. Among them, the shape evolution is less understood, despite the importance of morphology for NP properties. Here, we propose a symmetry-based kinematic theory (SBKT) based on classical growth theories to illustrate the process. Based on the crystal lattice, nucleus (or seed) symmetry, and the preferential growth directions under the experimental conditions, the SBKT can illustrate the growth trajectories. The theory accommodates the conventional criteria of the major existing theories for crystal growth and provides tools to better understand the symmetry-breaking process during the growth of anisotropic structures. Furthermore, complex dendritic growth is theoretically and experimentally demonstrated. Thus, it provides a framework to explain the shape evolution, and extends the morphogenesis prediction to cases, which cannot be treated by other theories.

15.
Nanoscale ; 14(8): 2990-2997, 2022 Feb 24.
Artigo em Inglês | MEDLINE | ID: mdl-35166288

RESUMO

The traditional synthesis of ammonia is an industrial process with high energy consumption that is not environmentally friendly; thus, it is urgent to develop cost-effective approaches to synthesize ammonia under ambient conditions. In recent years, the photochemical synthesis of ammonia has become a hot research frontier. In this mini review, we summarize the recent advances in materials sciences for photocatalytic nitrogen fixation. Beyond nitrogen fixation, we talk about an alternative for artificial ammonia synthesis and coupling reactions with other reactions for the synthesis of other high-value chemicals. The results and findings of this review will help the development of ammonia synthesis and the synthesis of other high-value chemicals.

16.
Bioresour Technol ; 349: 126887, 2022 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-35202830

RESUMO

While sulfur-driven autotrophic denitrification (SDAD) occurring in the anoxic reactor of the sulfate reduction, autotrophic denitrification and nitrification integrated (SANI) system has been regarded as the main nitrogen removal bioprocess, little is known about the accompanying Anammox bacteria whose presence is made possible by the co-existence of NH4+ and NO2-. Therefore, this work firstly developed an integrated SDAD-Anammox model to describe the interactions between sulfur-oxidizing bacteria and Anammox bacteria. The model was subsequently used to explore the impacts of influent conditions on the reactor performance and microbial community structure of the anoxic reactor. The results revealed that at a relatively low ratio of <1.5 mg S/mg N, Anammox bacteria could survive and even take a dominant position (up to 58.9%). Finally, a modified SANI system configuration based on the effective collaboration between SDAD and Anammox processes was proposed to improve the efficiency of the treatment of sulfate-rich saline sewage.


Assuntos
Reatores Biológicos , Desnitrificação , Processos Autotróficos , Nitrogênio , Oxirredução , Esgotos/microbiologia , Enxofre , Águas Residuárias/análise
17.
J Hazard Mater ; 430: 128195, 2022 May 15.
Artigo em Inglês | MEDLINE | ID: mdl-35180518

RESUMO

BiOX (X = Cl, Br and I) and BiOX/TiO2 photocatalysts were prepared by a facile hydrothermal approach. The BiOX/TiO2 heterojunctions demonstrated significantly enhanced efficiency for photocatalytic decomposition of perfluorooctanoic acid (PFOA) compared with sole BiOX or TiO2. PFOA (10 mg L1) was completely degraded by BiOCl(Br)/TiO2 in 8 h. Moreover, BiOCl/TiO2 attained deep decomposition of PFOA with a high defluorination ratio of 82%. The p-n heterojunctions between BiOX and TiO2 were confirmed by a series of characterizations. The photo-induced holes would migrate from the valance band (VB) of TiO2 to BiOX, driven by the built-in electric field (BIEF) near the interfaces of p-n heterojunctions, the inner electric fields (IEF) in BiOX and the higher VB position of BiOX. The X-ray diffraction (XRD) and TEM characterizations indicated that TiO2 combined with BiOX along the [110] facet, which facilitated photo-induced electron transfer in the [001] direction, thus benefiting PFOA decomposition.

18.
Arthritis Res Ther ; 24(1): 37, 2022 02 04.
Artigo em Inglês | MEDLINE | ID: mdl-35120571

RESUMO

BACKGROUND: Certain circRNAs could be used as biomarkers to determine the risk of development and/or severity of systemic lupus erythematosus, and their new function in the regulation of gene expression has motivated us to investigate their role in SLE METHODS: Experimental methods including qRT-PCR, RNA immunoprecipitation (RIP), pulldown, dual luciferase reporter assay, RNA interference and cell transfection, RNA fluorescence in situ hybridization, western blotting, and mass spectrometry were used to assessed circGARS (hsa_circRNA_0009000) for immune functions and defined mechanisms by which circGARS promotes the progression in SLE. RESULTS: Our results demonstrated that the levels of circGARS was remarkably upregulated in SLE and correlated with clinicopathological features. CircGARS directly combined with microRNA-19a (miR-19a). Functionally, circGARS downregulated the expression of TNFAIP3 (A20, tumor necrosis factor alpha-induced protein 3) to mediate the activation of immune responses that were regulated by the nuclear factor-κB (NF-κB) pathway as a negative feedback mechanism. In addition, miR-19a regulated A20 (TNFAIP3) degradation by downregulating the expression of YTH N6-methyladenosine RNA-binding protein 2 (YTHDF2). CONCLUSIONS: The circGARS sponges miR-19a to regulate YTHDF2 expression to promote SLE progression through the A20/NF-κB axis and may act as an independent biomarker to help the treatment of SLE patients.


Assuntos
Lúpus Eritematoso Sistêmico , NF-kappa B , Proteína 3 Induzida por Fator de Necrose Tumoral alfa/metabolismo , Adenosina/análogos & derivados , Humanos , Hibridização in Situ Fluorescente , Lúpus Eritematoso Sistêmico/genética , NF-kappa B/metabolismo , RNA Circular/genética , Proteína 3 Induzida por Fator de Necrose Tumoral alfa/genética
19.
Environ Sci Technol ; 56(4): 2816-2826, 2022 02 15.
Artigo em Inglês | MEDLINE | ID: mdl-35107268

RESUMO

Mathematical modeling plays a critical role toward the mitigation of nitrous oxide (N2O) emissions from wastewater treatment plants (WWTPs). In this work, we proposed a novel hybrid modeling approach by integrating the first principal model with deep learning techniques to predict N2O emissions. The hybrid model was successfully implemented and validated with the N2O emission data from a full-scale WWTP. This hybrid model is demonstrated to have higher accuracy for N2O emission modeling in the WWTP than the mechanistic model or pure deep learning model. Equally important, the hybrid model is more applicable than the pure deep learning model due to the lower requirement of data and the pure mechanistic model due to the less calibration requirement. This superior performance was due to the hybrid nature of the proposed model. It integrated the essential wastewater treatment knowledge as the first principal component and the less understood N2O production processes by the data-driven deep learning approach. The developed hybrid model was also successfully implemented under different circumstances for the prediction of N2O flux, which showed the generalizability of the model. The hybrid model also showed great potential to be applied for the N2O mitigation work. Nevertheless, the capability of the hybrid model in evaluating N2O mitigation strategies still requires validation with experiments. Going beyond N2O modeling in WWTP, the novel hybridization modeling concept can potentially be applied to other environmental systems.


Assuntos
Aprendizado Profundo , Purificação da Água , Modelos Teóricos , Óxido Nitroso/análise , Águas Residuárias , Purificação da Água/métodos
20.
Pain Physician ; 25(1): E87-E94, 2022 01.
Artigo em Inglês | MEDLINE | ID: mdl-35051155

RESUMO

BACKGROUND: Endoscopic rhizotomy (ER) of the medial branch has been recently developed for the treatment of lumbar facet joint pain (LFJP). However, there are no studies comparing the pain-free duration after ER and radiofrequency (RF). OBJECTIVES: To evaluate the long-term outcomes for pain and physical function in patients who underwent ER versus RF for LFJP and compare their pain-free survival. STUDY DESIGN: Open label, prospective, real-world study that includes patients treated with ER or RF at a single center between November 2017 and February 2020. SETTING: The research took place within a single university-based neuro-spine center. METHODS: Patients with a positive diagnostic medial branch block (>= 80% pain relief) were treated with ER or RF. Numeric rating scale (NRS), Oswestry Disability Index (ODI), and Global Impression of Change (GIoC) were obtained at the baseline, and at 6 months and 12 months postoperatively. The duration of pain-free time was recorded at every follow-up. The final follow-up was conducted in March 2021. RESULTS: Of 55 patients with LFJP, 19 underwent ER, and 36 underwent RF. Both ER and RF groups showed significant decreases in NRS and ODI scores at 6 months and 12 months compared with baseline (P < 0.001). ER had significantly better efficacy than RF in NRS, ODI, and GIoC scores at 6 and 12 months (P < 0.05). The pain-free survival curves showed that the median pain-free duration was 20 months and 10 months in ER and RF, respectively. LIMITATION: Patients were not randomized to different groups, which may have led to bias. CONCLUSIONS: Both ER and RF can improve the pain and physical function in patients with LFJP. ER is associated with a longer operative duration and medical expenses; however, it provides more sustained efficacy than RF. The surgical choice should depend on the patients' specific conditions.


Assuntos
Dor Lombar , Articulação Zigapofisária , Humanos , Dor Lombar/terapia , Vértebras Lombares/cirurgia , Estudos Prospectivos , Rizotomia , Articulação Zigapofisária/cirurgia
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