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1.
Sci Rep ; 12(1): 7738, 2022 May 11.
Artigo em Inglês | MEDLINE | ID: mdl-35545645

RESUMO

The precise identification of postural behavior plays a crucial role in evaluation of animal welfare and captive management. Deep learning technology has been widely used in automatic behavior recognition of wild and domestic fauna species. The Asian slow loris is a group of small, nocturnal primates with a distinctive locomotion mode, and a large number of individuals were confiscated into captive settings due to illegal trade, making the species an ideal as a model for postural behavior monitoring. Captive animals may suffer from being housed in an inappropriate environment and may display abnormal behavior patterns. Traditional data collection methods are time-consuming and laborious, impeding efforts to improve lorises' captive welfare and to develop effective reintroduction strategies. This study established the first human-labeled postural behavior dataset of slow lorises and used deep learning technology to recognize postural behavior based on object detection and semantic segmentation. The precision of the classification based on YOLOv5 reached 95.1%. The Dilated Residual Networks (DRN) feature extraction network showed the best performance in semantic segmentation, and the classification accuracy reached 95.2%. The results imply that computer automatic identification of postural behavior may offer advantages in assessing animal activity and can be applied to other nocturnal taxa.

2.
Mitochondrial DNA B Resour ; 7(1): 144-152, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35005229

RESUMO

Chrysolophus amherstiae and Chrysolophus pictus are two related species of Chrysolophus. Understanding the differences in their mitochondrial genome structure is of great significance for studying their phylogenetic relationship. In this study, the full mitochondrial genome of C. amherstiae was sequenced and annotated and analyzed with C. pictus to reveal their structural differences. At the same time, the phylogenetic trees were constructed based on the BI and ML methods with other species of the same family to analyze the phylogenetic relationship of Phasianidae. The results showed that the basic characteristics of mitochondrial genomes of C. amherstiae and C. pictus were similar. By comparing the secondary structures of rRNA and the relative synonymous codon usage of protein-coding genes, the subtle differences of mitochondrial structures between the two were further demonstrated.

3.
Int J Parasitol Parasites Wildl ; 18: 61-67, 2022 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-35492574

RESUMO

Enterocytozoon bieneusi is a zoonotic pathogen with a wide range of animal host. There are only few reports of E. bieneusi infection in wild Chinese rhesus macaques. Here, we determined the prevalence of E. bieneusi in nine wild rhesus macaque populations and assessed their zoonotic potential by performed genotype of ITS gene. A total of 324 fecal samples of rhesus macaque were collected in nine geographical populations from five Chinese provinces (Sichuan, Chongqing, Qinghai, Tibet and Hainan). 38 samples (11.7%) were found to be infected with E. bieneusi, and 11 genotypes were identified including three known genotypes (D, EbpC and SCC-2) and eight novel genotypes named Mul6∼13. Genotype D (63.2%) was the most prevalent, being observed in seven populations except XZ-2 and QH, and other genotypes were identified only in a single area. According to the phylogenetic analysis, Mul6∼9, Mul11∼13 and zoonotic genotype D were clustered into Group 1, indicating that these genotypes may be potentially zoonotic. Among nine populations, population SC-3 had the highest infection rate (26.3%), and the lowest was the wild QH population without infection, but the difference of infection rate among the nine populations is not significant. It is concluded that, rhesus macaque populations are generally infected E. bieneusi in many areas of China, and there may be a risk of cross infection with E. bieneusi in some areas found having zoonotic genotypes, and these areas should be paid more attention to prevent.

4.
Gigascience ; 10(9)2021 09 23.
Artigo em Inglês | MEDLINE | ID: mdl-34555848

RESUMO

BACKGROUND: The microbiota of the female reproductive tract is increasingly recognized as playing fundamental roles in animal reproduction. To explore the relative contribution of reproductive tract microbiomes to egg production in chickens, we investigated the microbiota in multiple reproductive and digestive tract sites from 128 female layer (egg-producing) chickens in comparable environments. RESULTS: We identified substantial differences between the diversity, composition, and predicted function of site-associated microbiota. Differences in reproductive tract microbiota were more strongly associated with egg production than those in the digestive tract. We identified 4 reproductive tract microbial species, Bacteroides fragilis, Bacteroides salanitronis, Bacteroides barnesiae, and Clostridium leptum, that were related to immune function and potentially contribute to enhanced egg production. CONCLUSIONS: These findings provide insights into the diverse microbiota characteristics of reproductive and digestive tracts and may help in designing strategies for controlling and manipulating chicken reproductive tract microbiota to improve egg production.


Assuntos
Galinhas , Microbiota , Animais , Feminino , Trato Gastrointestinal
5.
Front Microbiol ; 12: 650991, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33841376

RESUMO

Bengal slow lorises (Nycticebus bengalensis) are threatened by illegal trade. Subsequently, numerous wild-born individuals are rescued and transferred to rescue centers. Metabonomic analysis of intestinal microbiomes has increasingly played a vital role in evaluating the effects of dietary alteration on the captive status of endangered non-human primates. A synthetic analysis was done to test the differences in gut microbes and fecal metabolites between two dietary groups of Bengal slow lorises across 8 weeks. Dietary interventions led to intra-group convergence and inter-group variation in the composition of intestinal flora, metabolites, and short-chain fatty acids (SCFAs). The control diet, consisting of gums and honey, significantly increased the abundance of some potential probiotics, such as Bifidobacterium and Roseburia, and the concentration of some anti-disease related metabolites. The decrease in some amino acid metabolites in the original group fed without gums was attributed to poor body condition. Some distinct SCFAs found in the control group indicated the dietary alteration herein was fat-restricted but fiber deficient. Cognizant of this, plant exudates and fiber-enriched food supplies should be considered an optimal approach for dietary improvement of the confiscated and captive Bengal slow lorises.

6.
Aging (Albany NY) ; 13(1): 460-476, 2020 12 03.
Artigo em Inglês | MEDLINE | ID: mdl-33291074

RESUMO

Drugs or compounds have been shown to promote longevity in various approaches. We used Drosophila to explore novel natural compounds can be applied to anti-aging. Here we reported that a flavonoid named Dihydromyricetin can increase stress that tolerance and lipid levels, slow down gut dysfunction and extend Drosophila lifespan. Dihydromyricetin can also lessen pERK and pAKT signaling, consequently activating FOXO and AOP to modulate longevity. Our results suggested that DHM could be used as an effective compound for anti-aging intervention, which could likely be applied to both mammals and humans.


Assuntos
Envelhecimento/efeitos dos fármacos , Proteínas de Drosophila/efeitos dos fármacos , Proteínas do Olho/efeitos dos fármacos , Flavonóis/farmacologia , Fatores de Transcrição Forkhead/efeitos dos fármacos , Longevidade/efeitos dos fármacos , Proteínas Repressoras/efeitos dos fármacos , Envelhecimento/metabolismo , Animais , Proteínas de Drosophila/metabolismo , Drosophila melanogaster , Proteínas do Olho/metabolismo , Fatores de Transcrição Forkhead/metabolismo , Proteínas Repressoras/metabolismo
7.
Mitochondrial DNA B Resour ; 5(1): 824-825, 2020 Jan 24.
Artigo em Inglês | MEDLINE | ID: mdl-33366768

RESUMO

The complete mitochondrial of Cynops cyanurus cyanurus was determined by using Sanger sequencing and the mitogenome size of C. c. cyanurus was 16,465 bp. It contained 13 protein-coding genes, 22 tRNAs, 2 rRNAs, 1 control region (D-loop) and a non-coding region (NC). In addition, the phylogenetic tree shows that C. c. cyanurus has the closest relationship with C. c. chuxiongensis. Hoping this study can improve our understanding of the species evolutionary relationship of Cynops.

8.
Mitochondrial DNA B Resour ; 5(1): 1113-1114, 2020 Feb 11.
Artigo em Inglês | MEDLINE | ID: mdl-33366898

RESUMO

Red-and-white giant flying squirrel (Petaurista alborufus) is a widely distributed but rarely studied species. Here, we sequenced the complete mitochondrial genome about it, which is a circular genome of 16,511 bp and have a typical structure with mammals. The base composition shows higher composition of A\T. There are 61 variations and eight base deletions between two sequences when compared with the reported sequence (JQ743657). In this study, we obtain a new mitochondrial genome sequence of red-and-white giant flying squirrel, which provide novel molecular information that could prove fundamental to our understanding of diversity research and phylogenetic structure.

9.
Mitochondrial DNA B Resour ; 5(1): 1115-1116, 2020 Feb 11.
Artigo em Inglês | MEDLINE | ID: mdl-33366899

RESUMO

Ferret-badger (Melogale moschata) is a widely distributed and rarely studied species. Therefore, we sequenced its complete mitochondrial genome, which is circular in shape with 16,511 bp length and have a structure typical to mammals. There is 98.67% similarity when compared with the reported sequence (HM106328). It contained 13 protein-coding genes, 22 tRNA genes, 2 rRNA genes, and 1 control regions (D-loop). This study can provide novel molecular information and genetic diversity of ferret-badger.

10.
Mitochondrial DNA B Resour ; 5(1): 1117-1118, 2020 Feb 11.
Artigo em Inglês | MEDLINE | ID: mdl-33366900

RESUMO

The complete mitochondrial genome of the Oreolalax major (17,786 bp long) was obtained in this study. It includes 13 protein-coding genes (PCG), two ribosomal RNA (rRNA) genes, and 23 transfer RNA (tRNA) genes (GenBank accession number MN803320). The phylogenetic tree indicates that the O. major is closely related to the O. xiangchengensis.

11.
Mitochondrial DNA B Resour ; 5(1): 1122-1123, 2020 Feb 13.
Artigo em Inglês | MEDLINE | ID: mdl-33366902

RESUMO

Due to the multiple causes, the population of roe deer has declined significantly. In this study, we analyzed the complete mitogenome of Capreolus pygargus bedfordi, whose genome was 16,357 bp long. There were 13 protein-coding genes (PCG), two ribosomal RNA genes (12S rRNA and 16S rRNA), 22 transfer RNA genes, and one control region. Nine PCGs started with ATG, while NAD2, NAD3, and NAD5 genes commenced with ATA, and ND4L began with GTG. ND6 and eight tRNA genes were encoded on the L-strand. These results provide newer molecular information, which contribute to its molecular and phylogenetic studies, and genetic diversity conservation.

12.
Int J Parasitol Parasites Wildl ; 13: 191-195, 2020 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-33145162

RESUMO

Enterocytozoon bieneusi is an obligate intracellular parasite and the most common pathogen of microsporidiosis in humans and animals. In this study, a total of 198 fecal samples were collected from 11 captive populations of Rhesus macaque in Chinese zoos, to investigate the prevalence and analyze the zoonotic potential of E. bieneusi by genotype of the Internal Transcribed Spacer (ITS) gene on the rRNA of E. bieneusi via nested PCR. Results showed that the average infection rate of E. bieneusi in the 11 populations was 13.6%, and the highest infection rate was 56.5% in the population of Xinjiang Tianshan Zoo. Seven genotypes were identified including 2 known genotypes (D and CM1) and 5 novel genotypes (Mul1, Mul2, Mul3, Mul4 and Mul5). Phylogenetic analysis revealed that the novel genotypes Mul2, Mul3, Mul4 and Mul5 belonged to Group 1 showed the zoonotic potential.These findings extend the distribution of E. bieneusi genotypes and provide baseline data for controlling E. bieneusi infection.

13.
Aging (Albany NY) ; 12(22): 23082-23095, 2020 11 20.
Artigo em Inglês | MEDLINE | ID: mdl-33221768

RESUMO

Ubiquitination-mediated protein degradation is the selective degradation of diverse forms of damaged proteins that are tagged with ubiquitin, while deubiquitinating enzymes reverse ubiquitination-mediated protein degradation by removing the ubiquitin chain from the target protein. The interactions of ubiquitinating and deubiquitinating enzymes are required to maintain protein homeostasis. The ubiquitin-specific protease USP7 is a deubiquitinating enzyme that indirectly plays a role in repairing DNA damage and development. However, the mechanism of its participation in aging has not been fully explored. Regarding this issue, we found that USP7 was necessary to maintain the normal lifespan of Drosophila melanogaster, and knockdown of dusp7 shortened the lifespan and reduced the ability of Drosophila to cope with starvation, oxidative stress and heat stress. Furthermore, we showed that the ability of USP7 to regulate aging depends on the autophagy and ubiquitin signaling pathways. Furthermore, 2,5-dimethyl-celecoxib (DMC), a derivative of celecoxib, can partially restore the shortened lifespan and aberrant phenotypes caused by dusp7 knockdown. Our results suggest that USP7 is an important factor involved in the regulation of aging, and related components in this regulatory pathway may become new targets for anti-aging treatments.


Assuntos
Envelhecimento/metabolismo , Autofagia , Proteínas de Drosophila/metabolismo , Peptidase 7 Específica de Ubiquitina/metabolismo , Ubiquitinação , Animais , Drosophila melanogaster , Feminino , Técnicas de Silenciamento de Genes , Peptidase 7 Específica de Ubiquitina/genética
14.
Biomed Res Int ; 2020: 3852586, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32851066

RESUMO

Gene differential expression studies can serve to explore and understand the laws and characteristics of animal life activities, and the difference in gene expression between different animal tissues has been well demonstrated and studied. However, for the world-famous rare and protected species giant panda (Ailuropoda melanoleuca), only the transcriptome of the blood and spleen has been reported separately. Here, in order to explore the transcriptome differences between the different tissues of the giant panda, transcriptome profiles of the heart, liver, spleen, lung, and kidney from five captive giant pandas were constructed with Illumina HiSeq 2500 platform. The comparative analysis of the intertissue gene expression patterns was carried out based on the generated RNA sequencing datasets. Analyses of Gene Ontology (GO) enrichment, Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment, and protein-protein interaction (PPI) network were performed according to the identified differentially expressed genes (DEGs). We generated 194.52 GB clean base data from twenty-five sequencing libraries and identified 18,701 genes, including 3492 novel genes. With corrected p value <0.05 and |log2FoldChange| >2, we finally obtained 921, 553, 574, 457, and 638 tissue-specific DEGs in the heart, liver, spleen, lung, and kidney, respectively. In addition, we identified TTN, CAV3, LDB3, TRDN, and ACTN2 in the heart; FGA, AHSG, and SERPINC1 in the liver; CD19, CD79B, and IL21R in the spleen; NKX2-4 and SFTPB in the lung; GC and HRG in the kidney as hub genes in the PPI network. The results of the analyses showed a similar gene expression pattern between the spleen and lung. This study provided for the first time the heart, liver, lung, and kidney's transcriptome resources of the giant panda, and it provided a valuable resource for further genetic research or other potential research.


Assuntos
Genoma/genética , Distribuição Tecidual/genética , Transcriptoma/genética , Ursidae/genética , Animais , Perfilação da Expressão Gênica , Sequenciamento de Nucleotídeos em Larga Escala , Mapas de Interação de Proteínas/genética , Análise de Sequência de RNA/métodos
15.
Front Genet ; 11: 574, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32612636

RESUMO

The liver is the major organ of lipid biosynthesis in the chicken. In laying hens, the liver synthesizes most of the yolk precursors and transports them to developing follicles to produce eggs. However, a systematic investigation of the long non-coding RNA (lncRNA) and mRNA transcriptome in liver across developmental stages is needed. Here, we constructed 12 RNA libraries from liver tissue during four developmental stages: juvenile (day 60), sexual maturity (day 133), peak laying (day 220), and broodiness (day 400). A total of 16,930 putative lncRNAs and 18,260 mRNAs were identified. More than half (53.70%) of the lncRNAs were intergenic lncRNAs. The temporal expression pattern showed that lncRNAs were more restricted than mRNAs. We identified numerous differentially expressed lncRNAs and mRNAs by pairwise comparison between the four developmental stages and found that VTG2, RBP, and a novel protein-coding gene were differentially expressed in all stages. Time-series analysis showed that the modules with upregulated genes were involved in lipid metabolism processes. Co-expression networks suggested functional relatedness between mRNAs and lncRNAs; the DE-lncRNAs were mainly involved in lipid biosynthesis and metabolism processes. We showed that the liver transcriptome varies across different developmental stages. Our results improve our understanding of the molecular mechanisms underlying liver development in chickens.

16.
Front Genet ; 11: 570, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32655617

RESUMO

MicroRNA (miRNA) is known to be an important regulator of muscle growth and development. The regulation of microRNA on the skeletal muscle phenotype of animals is mainly achieved by regulating the proliferation and differentiation of myoblasts. In this study, we sequenced a total of 60 samples from 15 developing stages of the pectoral muscle and five other tissues at 300 days of Tibetan chicken. We characterized the expression patterns of miRNAs across muscle developmental stages, and found that the chicken growth and development stage was divided into early-embryonic and late-embryonic as well as postnatal stages. We identified 81 and 21 DE-miRNAs by comparing the miRNA profiles of pectoral muscle of three broad periods and different tissues, respectively; and 271 miRNAs showed time-course patterns. Their potential targets were predicted and used for functional enrichment to understand their regulatory functions. Significantly, GgmiRNA-454 is a time-dependent and tissue-differential expression miRNA. In order to elucidate the role of gga-miRNA-454 in the differentiation of myoblasts, we cultured chicken myoblasts in vitro. The results show that although gga-miRNA-454-3p initiates increase and thereafter decrease during the chicken myoblasts differentiation, it had no effect on primary myoblasts proliferation. Furthermore, we confirm that gga-miRNA-454 inhibits myoblast differentiation by targeting the myotube-associated protein SBF2.

17.
Conserv Biol ; 34(6): 1525-1535, 2020 12.
Artigo em Inglês | MEDLINE | ID: mdl-32484587

RESUMO

Seizures of hundreds of jaguar heads and canines in Central and South America from 2014 to 2018 resulted in worldwide media coverage suggesting that wildlife traffickers are trading jaguar body parts as substitutes for tiger parts to satisfy the demand for traditional Asian medicine. We compiled a data set of >1000 seized wild cats (jaguar [Panthera onca], puma [Puma concolor], and ocelot [Leopardus pardalis]) from 19 Central and South American countries and China. We ran generalized additive mixed models to detect trends in wild-cat seizures from 2012 to 2018 and assess the effects of socioeconomic factors of source countries and between those countries and China on the number of wild cats seized. Jaguar seizures increased over time, and most of the seized jaguar pieces were canines (1991 of 2117). Around 34% (32 of 93) of the jaguar-part seizure reports were linked with China, and these seizures contained 14-fold more individuals than those intended for domestic markets. Source countries with relatively high levels of corruption and Chinese private investment and low income per capita had 10-50 times more jaguar seizures than the remaining sampled countries. The number of Chinese residents in Central and South America was not significantly related to the number of jaguars seized. No socioeconomic factors influenced the seizures of puma and ocelots. Legal market chains may provide structure for the illegal chain; thus, the influx of illegal jaguar products is potentially a side effect of the economic partnership between Central and South American countries and China. Poverty and high levels of corruption in the source countries may motivate local people to engage in illegal activities and contribute to the growth of this trade. Supply-side interventions to curb this threat to Neotropical wild cats may include improved training for officials and promotion of governance and the value of protecting these animals to local people.


Mercado Ilegal de Felinos Silvestres y su Conexión al Desarrollo Encabezado por China en América Central y América del Sur Resumen La incautación de cientos de cabezas y colmillos de jaguar en América Central y América del Sur entre 2014 y 2018 resultó en una cobertura mediática mundial que sugirió que los traficantes de fauna están comerciando con partes de jaguar como sustituto de las partes de tigre para satisfacer la demanda de la medicina tradicional asiática. Recopilamos un conjunto de datos de más de mil felinos silvestres incautados (jaguar [Panthera onca], puma [Puma concolor], ocelote [Leopardus pardalis]) en 19 países de América Central y América del Sur y en China. Corrimos modelos aditivos mixtos generalizados para detectar las tendencias en las incautaciones de felinos silvestres entre 2012 y 2018 y para evaluar los efectos de los factores socioeconómicos de los países de origen y entre esos países y China sobre el número de felinos silvestres incautados. La incautación de artículos de jaguar incrementó con el tiempo y la mayoría de ellos fueron colmillos (1991 de 2117). Alrededor del 34% (32 de 93) de los reportes de incautación estuvieron vinculados a China y estas incautaciones tenían 14 veces más individuos que las incautaciones de artículos dirigidos al mercado doméstico. Los países de origen con niveles relativamente altos de corrupción y con inversión privada proveniente de China y con un bajo ingreso per cápita tuvieron de 10 a 50 veces más incautaciones de artículos de jaguar que los demás países muestreados. El número de residentes chinos en América Central y en América del Sur no tuvo una relación significativa con el número de jaguares incautados. Ningún factor socioeconómico influyó sobre las incautaciones de pumas y ocelotes. Las cadenas de mercado legales pueden proporcionar una estructura para la cadena ilegal; por lo tanto, la afluencia de productos ilegales de jaguar es potencialmente un efecto colateral de la colaboración económica entre China y los países de América Central y América del Sur. La pobreza y los altos niveles de corrupción en los países de origen pueden motivar a los habitantes locales a participar en actividades ilegales y a contribuir al crecimiento de este mercado. Las intervenciones del lado del suministro para disminuir esta amenaza para los felinos silvestres neotropicales pueden incluir mejoras al entrenamiento para los oficiales y el fomento entre los locatarios de la gestión y el valor de proteger a estos animales.


Assuntos
Panthera , Puma , Animais , Gatos , China , Conservação dos Recursos Naturais , Cães , América do Sul
18.
Dev Comp Immunol ; 109: 103718, 2020 08.
Artigo em Inglês | MEDLINE | ID: mdl-32360411

RESUMO

The chemokine CXCL16 and its receptor CXCR6 are implicated in various physiological and pathological processes in cooperative and/or stand-alone fashions. Despite the significance of rodent animal models in elucidating the function and clinical relevance of the chemokine and its receptor, the evolutionary characterization of these molecules remains deficient for this taxon to a certain extent. In this study, we implemented a comparison of synonymous and nonsynonymous variation rates in combination with the maximum likelihood (ML) analysis and Tajima's test to evaluate the interspecific and intraspecific evolutions of CXCL16 and CXCR6 in murine rodents. Our results indicate that adaptive selection has frequently contributed to genetic diversity of both CXCL16 and CXCR6 in the murine lineage that is asynchronous with a relative dependence between these genes. This signature is radically different from the lineage-specific and concordant adaptive diversity of the primate homologues of these genes, which was reported in a previous study. The diversity identified in the present study shed further light on molecular strategies against the challenges towards CXCL16 and CXCR6.


Assuntos
Quimiocina CXCL16/genética , Evolução Molecular , Receptores CXCR6/genética , Roedores/genética , Adaptação Fisiológica/genética , Animais , Quimiocina CXCL16/classificação , Bases de Dados Genéticas/estatística & dados numéricos , Camundongos , Filogenia , Ratos , Receptores CXCR6/classificação , Roedores/classificação , Especificidade da Espécie
19.
Toxins (Basel) ; 12(5)2020 05 04.
Artigo em Inglês | MEDLINE | ID: mdl-32375309

RESUMO

Aflatoxin B1 (AFB1) leads to a major risk to poultry and its residues in meat products can also pose serious threat to human health. In this study, after feeding 165-day-old Roman laying hens for 35 days, the toxic effects of aflatoxin B1 at different concentrations were evaluated. The purpose of this study was to explore the mechanism of liver toxicosis responses to AFB1. We found that highly toxic group exposure resulted in liver fat deposition, increased interstitial space, and hepatocyte apoptosis in laying hens. Furthermore, a total of 164 differentially expressed lnRNAs and 186 differentially expressed genes were found to be highly correlated (Pearson Correlation Coefficient > 0.80, p-value < 0.05) by sequencing the transcriptome of control (CB) and highly toxic group (TB3) chickens. We also identify 29 differentially expressed genes and 19 miRNAs that have targeted regulatory relationships. Based on the liver cell apoptosis and fatty liver syndrome that this research focused on, we found that the highly toxic AFB1 led to dysregulation of the expression of PPARG and BCL6. They are cis-regulated by TU10057 and TU45776, respectively. PPARG was the target gene of gga-miR-301a-3p, gga-miR-301b-3p, and BCL6 was the target gene of gga-miR-190a-3p. In summary, highly toxic AFB1 affects the expression levels of protein-coding genes and miRNAs in the liver of Roman layer hens, as well as the expression level of long non-coding RNA in the liver, which upregulates the expression of PPARG and downregulates the expression of Bcl-6. Our study provides information on possible genetic regulatory networks in AFB1-induced hepatic fat deposition and hepatocyte apoptosis.


Assuntos
Aflatoxina B1/toxicidade , Apoptose/efeitos dos fármacos , Hepatócitos/efeitos dos fármacos , Metabolismo dos Lipídeos/efeitos dos fármacos , Fígado/efeitos dos fármacos , RNA/metabolismo , Transcrição Genética/efeitos dos fármacos , Animais , Apoptose/genética , Galinhas , Feminino , Regulação da Expressão Gênica , Redes Reguladoras de Genes , Hepatócitos/metabolismo , Hepatócitos/patologia , Metabolismo dos Lipídeos/genética , Fígado/metabolismo , Fígado/patologia , MicroRNAs/genética , MicroRNAs/metabolismo , RNA/genética , RNA Longo não Codificante/genética , RNA Longo não Codificante/metabolismo , RNA Mensageiro/genética , RNA Mensageiro/metabolismo
20.
Curr Microbiol ; 77(10): 2623-2632, 2020 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-32440808

RESUMO

Gastrointestinal microbiome plays an important role in animal metabolism, immune system and pathology associated with health and disease. Many wild slow lorises were confiscated from illegal trade into captivities and experienced a range of changes in living environment and diet. Microbiome analysis contributes to improving captive management by identifying the alteration in their gastrointestinal microbial communities and aiding in determining the factors affecting the health of captive slow lorises. The fecal samples of eighteen Bengal slow lorises (Nycticebus bengalensis) were used to compare gut microbiota from four rescue centers located in Dehong, Gejiu, Nanning and Puer cities of China. The results showed a significant site-dependent difference in microbial community diversity. Similar to other Lorisinae species, the Phyla including Bacteroidetes, Firmicutes and Proteobacteria dominated their gut microbiome composition. The Gejiu group exhibited a higher overall diversity and the unique OTUs, which is resulted from long-term isolated husbandry and heavy human disturbances. The scarcity of gums in the captive diet was likely to cause a lower abundance of Prevotella associated with soluble fiber degradation. The variation of intestinal microbiota in different environments highlights the necessity to improve feed preparation and husbandry management for the captive Bengal slow lorises.


Assuntos
Microbioma Gastrointestinal , Lorisidae , Animais , China , Firmicutes/genética , Humanos , Proteobactérias/genética , RNA Ribossômico 16S/genética
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