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1.
PLoS Negl Trop Dis ; 15(4): e0009268, 2021 04.
Artigo em Inglês | MEDLINE | ID: mdl-33798191

RESUMO

BACKGROUND: Echinococcosis (canine Echinococcus disease) is a neglected tropical disease that causes serious public harm. Dogs, as a terminal host of Echinococcus spp., are a key part of the Echinococcus epidemic. Echinococcosis spreads easily in humans and animals in some areas of China and it is therefore necessary to fully understand the prevalence of Echinococcus spp. in dogs. METHODOLOGY/PRINCIPAL FINDINGS: PubMed, ScienceDirect, Chongqing VIP, China National Knowledge Infrastructure (CNKI), and WanFang databases were searched for relevant articles published in the past 10 years. A final total of 108 studies were included. The overall prevalence of Echinococcus spp. in dogs in China was 7.3%, with the highest point estimate found in sampling year 2015 (8.2%) and publication year 2015 (16.5%). Northwestern China (7.9%) had the highest infection rate in China. Qinghai Province (13.5%) showed the highest prevalence among the 11 provinces we included. We also found that geographical and climatic factors are related to the incidence of canine echinococcosis. We further investigated the source of heterogeneity by analysis of subgroups (sampling district, detection method, dog type, season, parasite species, medication, and study quality level). CONCLUSIONS/SIGNIFICANCE: Our research indicated that Echinococcus spp. were still prevalent in some areas in China. More localized prevention and control policies should be formulated, including improving drinking water hygiene and strengthening hygiene promotion. We recommend the rational use of anti-Echinococcus drugs. In addition, treatment of livestock offal and feces and improving the welfare of stray dogs may play an important role in reducing canine Echinococcus infections.


Assuntos
Doenças do Cão/epidemiologia , Equinococose/veterinária , Fezes/parasitologia , Animais , China/epidemiologia , Doenças do Cão/parasitologia , Cães , Equinococose/epidemiologia , Echinococcus/isolamento & purificação , Prevalência , Fatores de Risco
2.
Parasitol Res ; 120(5): 1617-1626, 2021 May.
Artigo em Inglês | MEDLINE | ID: mdl-33655350

RESUMO

Lysine crotonylation (Kcr) is an evolutionally conserved post-translational modification (PTM) on histone proteins. However, information about Kcr and its involvement in the biology and metabolism of Toxoplasma gondii is limited. In the present study, a global Kcr proteome analysis using LC-MS/MS in combination with immune-affinity method was performed. A total of 12,152 Kcr sites distributed over 2719 crotonylated proteins were identified. Consistent with lysine acetylation and succinylation in Apicomplexa, Kcr was associated with various metabolic pathways, including carbon metabolism, pyrimidine metabolism, glycolysis, gluconeogenesis, and proteasome. Markedly, many stage-specific proteins, histones, and histone-modifying enzymes related to the stage transition were found to have Kcr sites, suggesting a potential involvement of Kcr in the parasite stage transformation. Most components of the apical secretory organelles were identified as crotonylated proteins which were associated with the attachment, invasion, and replication of T. gondii. These results expanded our understanding of Kcr proteome and proposed new hypotheses for further research of the Kcr roles in the pathobiology of T. gondii infection.


Assuntos
Histonas/metabolismo , Lisina/metabolismo , Processamento de Proteína Pós-Traducional/genética , Proteínas de Protozoários/metabolismo , Toxoplasma/metabolismo , Acetilação , Cromatografia Líquida , Redes e Vias Metabólicas , Proteoma/metabolismo , Espectrometria de Massas em Tandem
3.
Parasitol Res ; 119(12): 4061-4071, 2020 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-33057814

RESUMO

Lysine 2-hydroxyisobutyrylation (Khib) is a recently discovered and evolutionarily conserved form of protein post-translational modification (PTM) found in mammalian and yeast cells. Previous studies have shown that Khib plays roles in the activity of gene transcription and Khib-containing proteins are closely related to the cellular metabolism. In this study, a global Khib-containing analysis using the latest databases (ToxoDB 46, 8322 sequences, downloaded on April 16, 2020) and sensitive immune-affinity enrichment coupled with liquid chromatography-tandem mass spectrometry was performed. A total of 1078 Khib modification sites across 400 Khib-containing proteins were identified in tachyzoites of Toxoplasma gondii RH strain. Bioinformatics and functional enrichment analysis showed that Khib-modified proteins were associated with various biological processes, such as ribosome, glycolysis/gluconeogenesis, and central carbon metabolism. Interestingly, many proteins of the secretory organelles (e.g., microneme, rhoptry, and dense granule) that play roles in the infection cycle of T. gondii were found to be Khib-modified, suggesting the involvement of Khib in key biological process during T. gondii infection. We also found that histone proteins, key enzymes related to cellular metabolism, and several glideosome components had Khib sites. These results expanded our understanding of the roles of Khib in T. gondii and should promote further investigations of how Khib regulates gene expression and key biological functions in T. gondii.


Assuntos
Regulação da Expressão Gênica/genética , Lisina/análogos & derivados , Processamento de Proteína Pós-Traducional/fisiologia , Proteínas de Protozoários/metabolismo , Toxoplasma/metabolismo , Acetilação , Animais , Carbono/metabolismo , Cromatografia de Afinidade , Cromatografia Líquida , Gluconeogênese/fisiologia , Glicólise/fisiologia , Histonas/metabolismo , Lisina/química , Espectrometria de Massas , Proteoma/análise , Proteínas de Protozoários/genética , Ribossomos/metabolismo , Toxoplasma/genética
4.
Microb Pathog ; 149: 104490, 2020 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-32956791

RESUMO

Porcine circovirus type 2 (PCV2) causes infectious diseases in pigs leading to considerable economic losses in the pig industry. To prevent and control PCV2 infections, is important to understand the prevalence and geographical distribution of the virus. We performed the first systematic review and meta-analysis to estimate the prevalence of PCV2 in China. From PubMed, ScienceDirect, Chinese Web of Knowledge, Wanfang, and VIP Chinese Journal, we extracted 53 studies published in China between 2015 and 2019. There were 29,051 samples, 14,230 of which were positive for PCV2. The pooled prevalence of PCV2 was 46.0%, with the highest in Northeastern China (58.1%). The highest prevalence was 86.3% in Xinjiang province. Nursery pigs had the highest prevalence of PCV2 (50.9%), and the serological test detected the highest number of cases (58.5%). PCV2 prevalence was 50.1% in intensive farms and 37.5% in extensive farms. Our findings showed that PCV2 is common throughout China. Effective control measures are necessary to reduce PCV2 infections.


Assuntos
Infecções por Circoviridae , Circovirus , Doenças dos Suínos , Animais , China/epidemiologia , Infecções por Circoviridae/epidemiologia , Infecções por Circoviridae/veterinária , Prevalência , Suínos , Doenças dos Suínos/epidemiologia
5.
Parasitol Res ; 119(6): 1873-1878, 2020 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-32377907

RESUMO

Enterocytozoon bieneusi is a common microsporidian species, which can infect humans and various species of animals. However, little is known about E. bieneusi prevalence and genotypes in farmed raccoon dogs (Nyctereutes procyonoides) in Shandong Province, China. In this study, a total of 356 fecal samples were collected from farmed raccoon dogs in Weihai, Weifang, and Yantai cities in Shandong Province, China. A total of 23 (6.5%) samples were E. bieneusi-positive by nested PCR amplification of the internal transcribed spacer (ITS) region of ribosomal DNA. Statistical analysis showed that E. bieneusi prevalence in male raccoon dogs was higher than that in female raccoon dogs, and the highest E. bieneusi prevalence was detected in adult raccoon dogs. Sequence analysis revealed four known E. bieneusi genotypes (D, type IV, CHG1, and Peru8), and type IV (11/23) was the predominant genotype. The genotypes type IV, Peru8, and CHG1 were reported in raccoon dogs for the first time in China. Phylogenetic analysis showed that three human-pathogenic genotypes (D, type IV, and Peru8) were clustered into group 1, and the CHG1 belonged to group 2. These findings expand the current understanding of E. bieneusi prevalence and genotype distribution in raccoon dogs in China. Our study also shows that raccoon dogs are hosts for E. bieneusi belonging to several genotypes, including zoonotic ones, highlighting the possibility of transmission of this pathogen between raccoon dogs and humans.


Assuntos
Enterocytozoon , Microsporidiose/veterinária , Cães Guaxinins/parasitologia , Animais , China/epidemiologia , Enterocytozoon/genética , Fazendas , Fezes/parasitologia , Feminino , Genótipo , Masculino , Microsporidiose/epidemiologia , Microsporidiose/parasitologia , Filogenia , Reação em Cadeia da Polimerase , Prevalência
6.
Front Microbiol ; 11: 776, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32411114

RESUMO

Lysine malonylation (Kmal) is a new post-translational modification (PTM), which has been reported in several prokaryotic and eukaryotic species. Although Kmal can regulate many and diverse biological processes in various organisms, knowledge about this important PTM in the apicomplexan parasite Toxoplasma gondii is limited. In this study, we performed the first global profiling of malonylated proteins in T. gondii tachyzoites using affinity enrichment and Liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis. Three experiments performed in tandem revealed 294, 345, 352 Kmal sites on 203, 236, 230 malonylated proteins, respectively. Computational analysis showed the identified malonylated proteins to be localized in various subcellular compartments and involved in many cellular functions, particularly mitochondrial function. Additionally, one conserved Kmal motif with a strong bias for cysteine was detected. Taken together, these findings provide the first report of Kmal profile in T. gondii and should be an important resource for studying the physiological roles of Kmal in this parasite.

7.
Microorganisms ; 8(3)2020 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-32121619

RESUMO

: In the present study, a dense granule protein 17 (gra17) and novel putative transporter (npt1) double deletion mutant of Toxoplasma gondii RH strain was engineered. The protective efficacy of vaccination using RHΔgra17Δnpt1 tachyzoites against acute, chronic, and congenital toxoplasmosis was studied in a mouse model. Immunization using RHΔgra17Δnpt1 induced a strong humoral and cellular response, as indicated by the increased levels of anti-T. gondii specific IgG, interleukin 2 (IL-2), IL-10, IL-12, and interferon-gamma (IFN-γ). Vaccinated mice were protected against a lethal challenge dose (103 tachyzoites) of wild-type homologous (RH) strain and heterologous (PYS and TgC7) strains, as well as against 100 tissue cysts or oocysts of Pru strain. Vaccination also conferred protection against chronic infection with 10 tissue cysts or oocysts of Pru strain, where the numbers of brain cysts in the vaccinated mice were significantly reduced compared to those detected in the control (unvaccinated + infected) mice. In addition, vaccination protected against congenital infection with 10 T. gondii Pru oocysts (administered orally on day 5 of gestation) as shown by the increased litter size, survival rate and the bodyweight of pups born to vaccinated dams compared to those born to unvaccinated + infected dams. The brain cyst burden of vaccinated dams was significantly lower than that of unvaccinated dams infected with oocysts. Our data show that T. gondii RHΔgra17Δnpt1 mutant strain can protect mice against acute, chronic, and congenital toxoplasmosis by balancing inflammatory response with immunogenicity.

8.
Parasitol Int ; 75: 102041, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-31846740

RESUMO

Toxoplasma gondii and Neospora caninum are two closely related protozoan parasites which can cause abortion and significant economic losses in sheep and goats. However, it is yet to know whether black-bone sheep and goats are infected with T. gondii and N. caninum in China. In the present investigation, the seroprevalence and risk factors of T. gondii and N. caninum infections in black-boned sheep and goats were investigated in Yunnan Province, subtropical southwest China between July and August of 2017. A total of 481 serum samples were tested for T. gondii antibodies using the Modified Agglutination Test (MAT), and 468 serum samples were examined for N. caninum antibodies by indirect Enzyme-Linked Immunosorbent Assay (iELISA). The overall seroprevalence of T. gondii in black-boned sheep and goats was 36.80% (177/481, 95% CI 32.49-41.11), and 40 out of 468 serum samples were N. caninum-seropositive (8.55%, 95% CI 6.02-11.08). There was significant difference in the seroprevalence of T. gondii infection in different regions (χ2 = 19.869, df = 2, P<0.01). As for the seroprevalence of N. caninum infection, region (χ2 = 8.558, df = 2, P<0.05), age (χ2 = 16.631, df = 3, P < 0.01), gender (χ2 = 11.219, df = 1, P < 0.01) and species (χ2 = 8.673, df = 1, P < 0.01) were the risk factors. In addition, the seroprevalence of coinfection of T. gondii and N. caninum in black-boned sheep and goats was 3.63% (17/468, 95% CI 1.94-5.32). To our knowledge, this is the first report of T. gondii and N. caninum seroprevalence in black-boned sheep and goats in China, which provided base-line data for the execution of control strategies and measures against T. gondii and N. caninum infection in black-boned sheep and goats.


Assuntos
Coccidiose/veterinária , Doenças das Cabras/epidemiologia , Neospora/isolamento & purificação , Doenças dos Ovinos/epidemiologia , Toxoplasma/isolamento & purificação , Toxoplasmose Animal/epidemiologia , Animais , China/epidemiologia , Coccidiose/epidemiologia , Coccidiose/parasitologia , Feminino , Doenças das Cabras/parasitologia , Cabras , Masculino , Prevalência , Fatores de Risco , Estudos Soroepidemiológicos , Ovinos , Doenças dos Ovinos/parasitologia , Toxoplasmose Animal/parasitologia
9.
Parasitol Res ; 119(1): 321-326, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31788771

RESUMO

Enterocytozoon bieneusi is an opportunistic enteric pathogen which can infect a wide range of animal species and humans. It is the most diagnosed species of Microsporidia in humans and has an impact on public health. Many infected animals including foxes may be a potential source for transmitting E. bieneusi to humans. However, limited information is available on the E. bieneusi prevalence and genotypes in farmed foxes in China. Therefore, in the present study, 344 fresh fecal samples were collected from farmed foxes (Vulpes vulpes and Vulpes lagopus) in Shandong Province, and the prevalence and genotypes of E. bieneusi were examined based on sequence analysis of the ribosomal internal transcribed spacer (ITS) region. The overall E. bieneusi prevalence was 9% (31/344); of them, 6.5% (9/138) in farmed silver foxes (V. vulpes) and 10.7% (22/206) in farmed arctic foxes (V. lagopus). Moreover, four known (Hum-q1, NCF2, HND-1, and Type IV) and two novel E. bieneusi genotypes (SDF1 and SDF2) were identified in farmed foxes in the present study. All of the E. bieneusi genotypes belonged to the zoonotic group based on phylogenetic analysis. In addition, 2, 4, 0, and 11 samples were successfully amplified at MS1, MS3, MS4, and MS7 loci, respectively. The present study reveals E. bieneusi prevalence and genotype distribution in farmed foxes in Shandong Province and enlarged the host and geographic information of E. bieneusi in China.


Assuntos
Enterocytozoon/genética , Enterocytozoon/isolamento & purificação , Raposas/microbiologia , Microsporidiose/veterinária , Animais , China/epidemiologia , DNA Espaçador Ribossômico/genética , Enterocytozoon/classificação , Fazendas , Fezes/microbiologia , Proteínas Fúngicas/genética , Genótipo , Microsporidiose/epidemiologia , Microsporidiose/microbiologia , Filogenia , Prevalência
10.
Acta Trop ; 192: 87-90, 2019 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-30738024

RESUMO

Hepatitis E virus (HEV) and influenza A virus (IAV) are two important pathogens which can infect humans and various animals causing public health problems. In this study, the seroprevalence and risk factors associated with HEV and IAV infection in farmed wild boars were investigated in China. A total of 758 serum samples were collected from farmed wild boars between 2015 and 2016, and antibodies against HEV and IAV were examined by enzyme-linked immunosorbent assay (ELISA) using commercially available kits. The overall prevalence of anti-HEV antibodies was 24.54% (186/758, 95% CI 21.48-27.60) in farmed wild boars. There were statistically significant differences in the HEV seroprevalence in farmed wild boars of different ages (<22 days: 8.33%; 22-66 days: 18.89%; >66 days: 26.36%) (P < 0.05) and different genders (50.00% in male and 23.49% in female) (P < 0.01). However, there was no statistically significant difference in the HEV seroprevalence in farmed wild boars of different regions and different years. The overall IAV seroprevalence was 5.80% (44/758, 95% CI 4.14-7.46), and there was no statistically significant difference in the IAV seroprevalence in farmed wild boars of different ages and genders, collected from different regions and different years. Our results indicate that HEV and IAV infections in farmed wild boars may pose a potential risk for human infection. To our knowledge, this is the first report of HEV and IAV seroprevalence in farmed wild boars in China, which provides baseline data for further studies and for control of HEV and IAV infection in farmed wild boars.


Assuntos
Animais Domésticos/virologia , Anticorpos Anti-Hepatite/sangue , Vírus da Hepatite E/imunologia , Vírus da Hepatite E/isolamento & purificação , Hepatite E/imunologia , Vírus da Influenza A/isolamento & purificação , Sus scrofa/virologia , Animais , China , Feminino , Hepatite E/epidemiologia , Humanos , Influenza Humana/epidemiologia , Masculino , Prevalência , Estudos Soroepidemiológicos , Suínos/virologia
11.
Acta Trop ; 190: 80-82, 2019 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-30408461

RESUMO

Neospora caninum is an intracellular protozoan parasite with a worldwide distribution, which can cause abortion and stillbirth in ruminants. However, little is known of N. caninum infection in raccoon dogs in China. Thus, a total of 1181 serum samples of farmed raccoon dogs were collected from the major production areas of Jilin, Changchun in Jilin province, and Rizhao, Weihai and Yantai in Shandong province in China. The antibodies to N. caninum were examined by a competitive-inhibition enzyme-linked immunoassay (cELISA). While the N. caninum seroprevalence in farmed raccoon dogs from different regions (cities) were not statistically significant, farmed raccoon dogs in Shandong province had a significantly higher N. caninum seroprevalence (8.99%, 95% CI 7.13-10.85) than those in Jilin province (4.46%, 95% CI 1.99-6.93) (P < 0.05). N. caninum seroprevalence in male and female raccoon dogs were 7.91% (95% CI 5.99-9.83) and 8.06% (95% CI 5.46-10.66), respectively. In addition, the seroprevalence of N. caninum infection in infancy, youth and adult raccoon dogs was 7.32% (95% CI 4.31-10.33), 7.93% (95% CI 5.44-10.42) and 8.41% (95% CI 5.82-11.00), respectively. This is the first report of N. caninum seroprevalence in farmed raccoon dogs in China, which provided baseline data for the prevention of N. caninum infection in raccoon dogs in China.


Assuntos
Anticorpos Antiprotozoários/sangue , Neospora/imunologia , Cães Guaxinins/parasitologia , Animais , China , Ensaio de Imunoadsorção Enzimática/veterinária , Feminino , Masculino , Neospora/isolamento & purificação , Estudos Soroepidemiológicos
12.
Artigo em Inglês | MEDLINE | ID: mdl-30211128

RESUMO

Infection with the apicomplexan protozoan parasite Toxoplasma gondii is an ongoing public health problem. The parasite's ability to invade and replicate within the host cell is dependent on many effectors, such as dense granule proteins (GRAs) released from the specialized organelle dense granules, into host cells. GRAs have emerged as important determinants of T. gondii pathogenesis. However, the functions of some GRAs remain undefined. In this study, we used CRISPR-Cas9 technique to disrupt 17 GRA genes (GRA11, GRA12 bis, GRA13, GRA14, GRA20, GRA21, GRA28-31, GRA33-38, and GRA40) in the virulent T. gondii RH strain. The CRISPR-Cas9 constructs abolished the expression of the 17 GRA genes. Functional characterization of single ΔGRA mutants was achieved in vitro using cell-based plaque assay and egress assay, and in vivo in BALB/c mice. Targeted deletion of these 17 GRA genes had no significant effect neither on the in vitro growth and egress of the mutant strains from the host cells nor on the parasite virulence in the mouse model of infection. Comparative analysis of the transcriptomics data of the 17 GRA genes suggest that GRAs may serve different functions in different genotypes and life cycle stages of the parasite. In sum, although these 17 GRAs might not be essential for RH strain growth in vitro or virulence in mice, they may have roles in other strains or parasite stages, which warrants further investigations.


Assuntos
Grânulos Citoplasmáticos/metabolismo , Proteínas de Protozoários/metabolismo , Toxoplasma/crescimento & desenvolvimento , Toxoplasma/metabolismo , Fatores de Virulência/metabolismo , Animais , Proteína 9 Associada à CRISPR , Repetições Palindrômicas Curtas Agrupadas e Regularmente Espaçadas , Grânulos Citoplasmáticos/genética , Modelos Animais de Doenças , Edição de Genes , Técnicas de Inativação de Genes , Camundongos , Camundongos Endogâmicos BALB C , Proteínas de Protozoários/genética , Toxoplasma/genética , Toxoplasma/patogenicidade , Toxoplasmose/parasitologia , Toxoplasmose/patologia , Virulência , Fatores de Virulência/genética
13.
Biomed Res Int ; 2018: 2098908, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-30003091

RESUMO

Neospora caninum is an intracellular protozoan parasite which can cause abortion and stillbirth in ruminants. However, there is no information on Tibetan sheep N. caninum infection in China. A total of 2187 serum samples were collected from Tibetan sheep in the major production areas of Luqu, Maqu, and Tianzhu in Gansu province, and Nyingchi in southeast Tibet, China. All samples were analyzed for the presence of antibodies to N. caninum using a competitive-inhibition enzyme-linked immunoassay. Of the 2187 serum samples, 184 (8.4%, 95% CI 7.3-9.6) were tested N. caninum seropositive. The N. caninum seroprevalence ranged from 4.4% (95% CI 1.4-7.4) to 11.3% (95% CI 8.2-14.4) among different regions, seasons, ages, and pregnancies, and there was no statistical significance among those groups (P > 0.05). Seroprevalence in male (10.8% 69/638) (95% CI 8.4-13.2) was significantly higher than in female (7.4% 115/1549) (OR =1.51, 95% CI 6.1-8.7) (P < 0.01). To our knowledge, this is the first report of N. caninum seroprevalence in Tibetan sheep in China, which provides baseline data for the prevention and control of N. caninum infection in Tibetan sheep.


Assuntos
Coccidiose/veterinária , Neospora/isolamento & purificação , Doenças dos Ovinos/parasitologia , Animais , Anticorpos Antiprotozoários , China , Ensaio de Imunoadsorção Enzimática , Feminino , Masculino , Gravidez , Fatores de Risco , Estudos Soroepidemiológicos , Ovinos , Tibet
14.
Vector Borne Zoonotic Dis ; 18(9): 504-508, 2018 09.
Artigo em Inglês | MEDLINE | ID: mdl-29688824

RESUMO

Chlamydia is Gram-negative obligate bacterium, which can cause human diseases worldwide and has huge economic impact on animals. It is yet to know whether farmed wild boars are infected with Chlamydia in China. To assess risk factors of Chlamydia infection in farmed wild boars in China, from April 2015 to February 2016, a total of 837 serum samples of farmed wild boars were collected in Jilin province, northeastern China, and antibodies against Chlamydia were examined by the indirect hemagglutination assay. The investigation showed that antibodies to Chlamydia were detected in 332 (39.67%, 95% CI 33.36-42.98) of 837 serum samples of farmed wild boars, seroprevalence ranged from 33.71% to 44.42% among different regions and the differences were statistically significant by SPSS analysis (p = 0.0248). These results indicated that Chlamydia is highly prevalent in farmed wild boars in Jilin province, northeastern China, and may pose a potential risk for human health. To our knowledge, this is the first report of Chlamydia seroprevalence in farmed wild boars in China, which provided baseline data for preventing and controlling Chlamydia infection in wild boars in China.


Assuntos
Criação de Animais Domésticos , Infecções por Chlamydia/veterinária , Chlamydia/isolamento & purificação , Sus scrofa/microbiologia , Doenças dos Suínos/microbiologia , Animais , Infecções por Chlamydia/epidemiologia , Infecções por Chlamydia/microbiologia , Suínos , Doenças dos Suínos/epidemiologia
15.
Parasite ; 25: 16, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-29557777

RESUMO

This study was conducted to detect specific anti-Neospora antibodies using a commercial competitive-inhibition ELISA kit, and to evaluate the risk factors for Neospora spp. infection. Out of a total of 2,228 donkey sera collected in three provinces in China, 211 (9.5%) were found to be positive for anti-Neospora antibodies. Statistical analysis revealed that age (p = 0.019, OR = 1.62, 95%CI: 1.08-2.44), feeding status (p < 0.001, OR = 3.79, 95%CI: 2.65-5.43), miscarriage history (p = 0.006, OR = 2.56, 95%CI: 1.27-4.01), and contact with dogs (p < 0.001, OR = 2.69, 95%CI: 1.86-3.88) were significant risk factors for Neospora spp. infection. This is the first evidence of Neospora infection in donkeys in China.


Assuntos
Anticorpos Antiprotozoários/sangue , Coccidiose/veterinária , Equidae/parasitologia , Neospora/imunologia , Aborto Animal/epidemiologia , Aborto Animal/parasitologia , Fatores Etários , Animais , China/epidemiologia , Coccidiose/epidemiologia , Cães , Ensaio de Imunoadsorção Enzimática/veterinária , Feminino , Modelos Logísticos , Masculino , Análise Multivariada , Kit de Reagentes para Diagnóstico/veterinária , Fatores de Risco , Estudos Soroepidemiológicos
16.
Acta Trop ; 182: 212-214, 2018 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-29545154

RESUMO

Porcine enzootic pneumonia caused by Mycoplasma hyopneumoniae affects the global pig industry with significant economic losses. It is yet to know whether wild boars in China were infected with M. hyopneumoniae. The present study was conducted to examine the seroprevalence and to evaluate risk factors of M. hyopneumoniae infection in farmed wild boars in China. A total of 882 serum samples were collected from farmed wild boars in Jilin City, Siping City and Baishan City in Jilin Province, northeastern China from April 2015 to February 2016, and were examined by the double sandwich enzyme-linked immunosorbent assay (ELISA). Seventy-eight out of 882 (8.8%) serum samples were M. hyopneumoniae-seropositive. Among region groups, wild boars from Jilin city (11.7%, 33/281) had the highest seropositivity, followed by Siping city (11%, 29/263) and Baishan city (4.7%, 16/338), and the difference was statistically significant (P = 0.0031). The M. hyopneumoniae seroprevalence in the female wild boars (9.0%, 75/831) was higher than that in the male wild boars (5.9%, 3/51) (P = 0.4429). The results of this investigation showed that farmed wild boars were susceptible to M. hyopneumoniae. Logistic regression analysis showed that there is a significant correlation between the geographical area and M. hyopneumoniae infection, which may be related to the regional environment. This is the first report of M. hyopneumoniae seroprevalence in farmed wild boars in China, which provided baseline information for further studies and control of M. hyopneumoniae infection in wild boars in China.


Assuntos
Anticorpos Antibacterianos/sangue , Mycoplasma hyopneumoniae/imunologia , Suínos/microbiologia , Animais , China , Ensaio de Imunoadsorção Enzimática/métodos , Feminino , Masculino , Estudos Soroepidemiológicos
17.
Infect Genet Evol ; 55: 172-174, 2017 11.
Artigo em Inglês | MEDLINE | ID: mdl-28842355

RESUMO

Toxoplasma gondii can infect nearly all warm-blooded animals including birds. However, limited information on the molecular epidemiology and genotypes of T. gondii infecting poultry is available in China. Therefore, the present study characterized T. gondii genotypes in poultry meat in eastern China. During August 2015 and September 2016, muscle tissue samples collecting from 414 poultries (257 chickens, 115 ducks and 42 geese) in Shandong provinces were used to detect the T. gondii B1 gene by a semi-nested PCR, and the positive samples were genotyped at 10 nuclear loci (i.e., SAG1, alternative SAG2, 5'- and 3'-SAG2, SAG3, L358, BTUB, c22-8, GRA6, c29-2, PK1) and an apicoplast locus Apico by multi-locus PCR-RFLP technology. Thirty-two (7.37%) samples from 414 poultry meat were T. gondii B1 gene positive. Chicken had the highest T. gondii prevalence (8.17%), followed by ducks (7.83%) and geese (4.76%). Furthermore, only one genotype (ToxoDB#9) was identified. This is the first genetic characterization of T. gondii isolates from poultry meat in Shandong province, eastern China and also the first report of genotype ToxoDB#9 was found in poultry in China, which provide basic data for the surveillance and control of T. gondii infection in poultry, other animals and humans.


Assuntos
Parasitologia de Alimentos , Produtos Avícolas/parasitologia , Toxoplasma/genética , Toxoplasmose Animal/parasitologia , Animais , China/epidemiologia , Prevalência , Vigilância em Saúde Pública
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