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1.
Science ; 375(6576): eaaw9021, 2022 Jan 07.
Artigo em Inglês | MEDLINE | ID: mdl-34990240

RESUMO

Epithelial organoids are stem cell­derived tissues that approximate aspects of real organs, and thus they have potential as powerful tools in basic and translational research. By definition, they self-organize, but the structures formed are often heterogeneous and irreproducible, which limits their use in the lab and clinic. We describe methodologies for spatially and temporally controlling organoid formation, thereby rendering a stochastic process more deterministic. Bioengineered stem cell microenvironments are used to specify the initial geometry of intestinal organoids, which in turn controls their patterning and crypt formation. We leveraged the reproducibility and predictability of the culture to identify the underlying mechanisms of epithelial patterning, which may contribute to reinforcing intestinal regionalization in vivo. By controlling organoid culture, we demonstrate how these structures can be used to answer questions not readily addressable with the standard, more variable, organoid models.


Assuntos
Mucosa Intestinal/crescimento & desenvolvimento , Organogênese , Organoides/crescimento & desenvolvimento , Engenharia Tecidual , Animais , Diferenciação Celular , Forma Celular , Células Epiteliais/citologia , Hidrogéis , Mucosa Intestinal/anatomia & histologia , Mucosa Intestinal/citologia , Mucosa Intestinal/metabolismo , Camundongos , Organoides/anatomia & histologia , Organoides/citologia , Organoides/metabolismo , Celulas de Paneth/citologia , Receptores Notch/metabolismo , Transdução de Sinais , Células-Tronco/citologia , Células-Tronco/fisiologia , Técnicas de Cultura de Tecidos , /metabolismo
2.
Parkinsonism Relat Disord ; 84: 112-121, 2021 03.
Artigo em Inglês | MEDLINE | ID: mdl-33609962

RESUMO

Mutations in the glucocerebrosidase gene (GBA) encoding the lysosomal enzyme glucocerebrosidase (GCase) cause Gaucher disease (GD) and are the most commonly known genetic risk factor for Parkinson disease (PD). Ambroxol is one of the most effective pharmacological chaperones of GCase. Fourteen GD patients, six PD patients with mutations in the GBA gene (GBA-PD), and thirty controls were enrolled. GCase activity and hexosylsphingosine (HexSph) concentration were measured in dried blood and macrophage spots using liquid chromatography coupled with tandem mass spectrometry. The effect of ambroxol on GCase translocation to lysosomes was assessed using confocal microscopy. The results showed that ambroxol treatment significantly increased GCase activity in cultured macrophages derived from patient blood monocytic cell (PBMC) of GD (by 3.3-fold) and GBA-PD patients (by 3.5-fold) compared to untreated cells (p < 0.0001 and p < 0.0001, respectively) four days after cultivation. Ambroxol treatment significantly reduced HexSph concentration in GD (by 2.1-fold) and GBA-PD patients (by 1.6-fold) (p < 0.0001 and p < 0.0001, respectively). GD macrophage treatment resulted in increased GCase level and increased enzyme colocalization with the lysosomal marker LAMP2. The possible binding modes of ambroxol to mutant GCase carrying N370S amino acid substitution at pH 4.7 were examined using molecular docking and molecular dynamics simulations. The ambroxol position characterized by minimal binding free energy was observed in close vicinity to the residue, at position 370. Taken together, these data showed that PBMC-derived macrophages could be used for assessing ambroxol therapy response for GD patients and also for GBA-PD patients.


Assuntos
Ambroxol/farmacologia , Inibidores Enzimáticos/farmacologia , Doença de Gaucher/tratamento farmacológico , Glucosilceramidase/efeitos dos fármacos , Macrófagos/efeitos dos fármacos , Chaperonas Moleculares/farmacologia , Doença de Parkinson/tratamento farmacológico , Translocação Genética/efeitos dos fármacos , Adulto , Idoso , Idoso de 80 Anos ou mais , Células Cultivadas , Feminino , Glucosilceramidase/antagonistas & inibidores , Humanos , Masculino , Pessoa de Meia-Idade
3.
J Clin Neurosci ; 78: 323-326, 2020 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-32336641

RESUMO

Immune response may play a pivotal role in the pathogenesis of the common synucleinopathy as Parkinson's disease (PD) and could be mediated with the accumulation of neurotoxic alpha-synuclein. There is limited evidence for immune response in another synucleinopathy as dementia with Lewy bodies (DLB). Recent data suggest that immune response may contribute to cognitive impairment. We aimed to estimate plasma cytokine profile in patients with synucleinopathies with dementia (PD dementia (PDD), DLB). Plasma cytokine levels (interferon-gamma (IFN-gamma), interleukin (IL)-4 (IL-4), IL-6, IL-10, tumor necrosis factor alpha (TNF-alpha), monocyte chemoattractant protein-1 (MCP-1)). were estimated in 16 patients with DLB, 19 patients with PDD, 28 patients with PD without dementia (PD) and 19 individuals without neurological disorders (controls) using Luminex array system. Cognitive status was assessed with the Mini-Mental State Examination (MMSE). TNF-alpha and IL-6 plasma levels were elevated in patients with synucleinopathies with dementia (DLB, PDD) compared to controls and IL-10 plasma level was increased in PDD compared to controls (p < 0.05). IFN-gamma levels were decreased in PD and PDD patients compared to controls (p < 0.001, p = 0.026, respectively) and in PD patients than in DLB patients (p = 0.032). Patients with PD, PDD, and DLB were characterized by increased plasma levels of MCP-1 compared to controls (p < 0.001). At the same time, no differences in TNF-alpha, IL-10, IL-6 plasma levels in PD patients compared to controls were found. Our study demonstrated more pronounced immune response in synucleinopathies associated with dementia compared to PD without demetia.


Assuntos
Citocinas/sangue , Demência/etiologia , Sinucleinopatias/imunologia , Idoso , Idoso de 80 Anos ou mais , Quimiocina CCL2/sangue , Demência/sangue , Demência/imunologia , Feminino , Humanos , Interferon gama/sangue , Interleucina-10/sangue , Interleucina-6/sangue , Doença por Corpos de Lewy/sangue , Doença por Corpos de Lewy/imunologia , Testes de Estado Mental e Demência , Pessoa de Meia-Idade , Doença de Parkinson/sangue , Doença de Parkinson/complicações , Doença de Parkinson/imunologia , Sinucleinopatias/sangue , Sinucleinopatias/complicações , Fator de Necrose Tumoral alfa/sangue
4.
Bull Exp Biol Med ; 168(4): 423-426, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-32146630

RESUMO

Plasma cytokine concentration in patients with Parkinson's disease and mutation in GBA gene, in patients with sporadic Parkinson's disease, and in healthy volunteers were measured by ELISA and multiplex analysis. In patients with Parkinson's disease and mutation in GBA gene, elevated plasma concentrations of IL-1ß and TNFα were revealed by ELISA in comparison with both controls and patients with sporadic form of Parkinson's disease. Multiplex analysis revealed enhanced secretion of IL-1ß, IL-2, IFNγ and reduced plasma levels of monocyte chemoattractant protein-1 (MCP-1) in patients with Parkinson's disease and mutation in GBA gene (in comparison with other groups) and increased plasma levels of IL-13 (only in comparison with the healthy volunteers). Our results support the hypothesis that the concentrations of inflammatory mediators are increased in patients with Parkinson's disease and mutation in GBA gene.


Assuntos
Glucosilceramidase/genética , Mutação , Doença de Parkinson/genética , Idoso , Estudos de Casos e Controles , Quimiocina CCL2/sangue , Quimiocina CCL2/genética , Quimiocina CCL2/imunologia , Ensaio de Imunoadsorção Enzimática , Feminino , Expressão Gênica , Glucosilceramidase/sangue , Glucosilceramidase/imunologia , Humanos , Inflamação , Interferon gama/sangue , Interferon gama/genética , Interferon gama/imunologia , Interleucina-13/sangue , Interleucina-13/genética , Interleucina-13/imunologia , Interleucina-1beta/sangue , Interleucina-1beta/genética , Interleucina-1beta/imunologia , Interleucina-2/sangue , Interleucina-2/genética , Interleucina-2/imunologia , Masculino , Pessoa de Meia-Idade , Doença de Parkinson/sangue , Doença de Parkinson/imunologia , Doença de Parkinson/patologia , Fator de Necrose Tumoral alfa/sangue , Fator de Necrose Tumoral alfa/genética , Fator de Necrose Tumoral alfa/imunologia
5.
Mol Biol (Mosk) ; 53(1): 28-36, 2019.
Artigo em Russo | MEDLINE | ID: mdl-30895950

RESUMO

Parkinson's disease (PD) characterized with slow continuous degeneration of dopaminergic neurons in the substantia nigra is one of the most common neurodegenerative diseases, but its etiology and pathogenesis are not fully understood. The pathogenesis of PD involves the impairment of lysosomal autophagy, which also contributes to lysosomal storage disorders (LSDs). In this work, the expression of genes related to lysosomal autophagy: Hspa8, Lamp2, Tfam, Slc18a2, and Vps35, was analyzed in the brain tissues of mice with the earliest stage of MPTP-induced PD. The detected decrease in Hspa8 and Lamp2 mRNA levels suggests that dysfunction of lysosomal autophagy maybe involved in the earliest stages of PD pathogenesis. A decrease in the rate of lysosomal autophagy may affect the accumulation of damaged proteins and the formation of protein inclusions in PD. Genes related to the lysosome function may be involved in development of both LSD and PD at the earliest stages of these pathophysiological processes.


Assuntos
Proteínas de Choque Térmico HSC70/genética , Doenças por Armazenamento dos Lisossomos/genética , Proteína 2 de Membrana Associada ao Lisossomo/genética , Doença de Parkinson/patologia , Animais , Autofagia , Lisossomos/patologia , Camundongos , Doença de Parkinson/genética
6.
J Neurol Sci ; 395: 135-140, 2018 12 15.
Artigo em Inglês | MEDLINE | ID: mdl-30316070

RESUMO

Parkinson's disease (PD) is the second most frequent neurodegenerative disorder. Impaired metabolism of alpha-synuclein (SNCA) and its aggregation are implicated in PD pathogenesis. SNCA has been identified as a highly significant genetic risk loci associated with the sporadic form of PD in across populations in GWAS and replicative studies. In this study we conducted a genetic analysis of five SNCA single nucleotide polymorphisms (SNPs) (rs356219, rs2619364, rs11931074, rs2583988, rs356168) in 458 PD patients and 353 from North-West region of Russia. We also assessed an association of studied SNPs with alpha-synuclein levels in homogeneous cell fraction of CD45+ blood cells in PD patients and controls. An association with PD was shown for SNPs rs356219, rs11931074, rs356168. After correction for covariates the significant association with the disease only for rs11931074 and rs356168 was shown. Alpha-synuclein level in peripheral blood CD45+ cells was significantly increased in PD patients compared to control subjects (р = 0.02). The effect of SNCA rs356219 and rs356168 on CD45+ alpha-synuclein level in PD patients and control groups was shown. At the same tame the increase of CD45+ alpha-synuclein level in PD patients was revealed only in risk allele carriers as for rs356219 and rs356168 SNPs. Therefore, our study was the first that demonstrated the increased level of alpha-synuclein in CD45+ blood cells in PD patients and showed that it could be influenced by SNCA rs356168 and rs356219. In conclusion we confirmed the significance of the SNCA locus in the PD development.


Assuntos
Doença de Parkinson/sangue , Doença de Parkinson/genética , Polimorfismo de Nucleotídeo Único , alfa-Sinucleína/sangue , alfa-Sinucleína/genética , Idoso , Biomarcadores/sangue , Células Sanguíneas/metabolismo , Estudos de Casos e Controles , Feminino , Estudos de Associação Genética , Predisposição Genética para Doença , Humanos , Antígenos Comuns de Leucócito/metabolismo , Desequilíbrio de Ligação , Masculino , Pessoa de Meia-Idade
7.
Artigo em Russo | MEDLINE | ID: mdl-29171494

RESUMO

BACKGROUND: Mutations in the glucocerebrosidase gene (GBA) increase the risk of Parkinson's disease (PD) by 6-10 times in all populations and are associated with the early-onset of PD, development of cognitive impairment and presence of psychotic disorders. At the same time, polymorphic variants associated with the twofold increase in the risk of PD were also described in the GBA gene. AIM: To estimate the clinical features of PD in patients with mutations and polymorphic variants of the GBA gene. MATERIAL AND METHODS: Evaluation of motor, cognitive, emotional, psychotic and autonomic dysfunctions in patients with mutations (N370S, L444P) and polymorphic variants (E326K, T369M) in the GBA gene was performed using clinical scales. RESULTS: Patients with mutations (mGBA-PD), and with polymorphic variants (pGBA-PD) in the GBA gene were compared with the group of patients with sporadic PD (sPD). Compared to sPD, affective disorders (depression and anxiety) were more expressed in the mGBA-PD group (p=0.001) and the general GBA-PD group (p=0.001) assessed with Sheehan anxiety rating scale, in the pGBA-PD group (p=0.012) and the general GBA-PD group (p=0.05) assessed with the NPI, in the mGBA-PD (p=0.003), pGBA-PD (p=0.022), and general GBA-PD groups (p=0.001) assessed with the Hospital Anxiety and Depression scale (HADS 'A'), and in the pGBA-PD group (p=0.005) assessed with the HADS 'D'. Non-motor symptoms assessed with the PD-NMS were more expressed in the pGBA-PD patients (p=0.007) and in the total group with GBA-PD (p=0,014) compared to sPD. Cognitive impairment measured with MMSE was more marked in mGBA-PD patients (p=0.022). Differences in motor and non-motor clinical symptoms between pGBA-PD and mGBA-PD groups were not found. CONCLUSION: Thus, clinical features of non-motor symptoms were described both in carriers of GBA mutations and polymorphisms. Identification of the specific clinical phenotype of PD in carriers of GBA polymorphic variants is important due to their relatively high prevalence in PD patients.


Assuntos
Glucosilceramidase/genética , Doença de Parkinson/genética , Doença de Parkinson/fisiopatologia , Adulto , Idoso , Idoso de 80 Anos ou mais , Doenças do Sistema Nervoso Autônomo/fisiopatologia , Disfunção Cognitiva/diagnóstico , Disfunção Cognitiva/genética , Emoções , Feminino , Heterozigoto , Humanos , Masculino , Pessoa de Meia-Idade , Mutação , Doença de Parkinson/psicologia , Fenótipo , Polimorfismo Genético
8.
Rev. Esp. Cir. Ortop. Traumatol. (Ed. Impr.) ; 61(4): 216-223, jul.-ago. 2017. tab, ilus
Artigo em Espanhol | IBECS | ID: ibc-164789

RESUMO

Objetivo. Evaluar los resultados de la reconstrucción artroscópica para el tratamiento de la seudoartrosis de escafoides inestable con autoinjerto de hueso esponjoso. Método. Se trató a 13 pacientes con una edad media de 26 (18-45) años. El tiempo medio desde la fractura hasta la intervención fue de 14 (6-48) meses. Se evaluaron parámetros clínicos y radiológicos preoperatorios y postoperatorios. El seguimiento medio fue de 16,8 (12-36) meses. Resultados. La consolidación se obtuvo en todos los casos a las 7 (4-10 semanas), ningún paciente presentó complicaciones ni precisó nuevas reintervenciones. El arco de movilidad, la dolor, la valoración funcional (cuestionario DASH) y las mediciones radiológicas mejoraron respecto al preoperatorio. El rango de movimiento promedio de flexión mejoró de 71,9° (55°-80°) a 81,7° (55°-90°), extensión de 66,3° (30°-80°) a 84,4° (70°-90°), desviación ulnar 21,5° (10°-25°) a 25,5° (20°-45°) y desviación radial 11,9° (5°-25°) a 13,3° (10°-20°). El dolor (EVA 0-10) mejoró de 6,8 (4-10) a 0,7 (0-3). Y la escala funcional DASH mejoró de 36 (12-78) a 8 (0-10). El ángulo escafolunar mejoró de 67,7° (62°-88°) a 47° (32°-55°) y el ángulo radiolunar mejoró de 30,8° (10°-45°) a 4° (0°-10°). Conclusión. El tratamiento de seudoartrosis de escafoides inestables con injerto esponjoso asistido por artroscopia presenta buenos resultados clínicos, con un corto tiempo de consolidación y recuperación (AU)


Objective. To evaluate the results of arthroscopic reconstruction for the treatment of unstable scaphoid non-union with cancellous bone autograft. Methods. 13 patients were treated with a mean age of 26 (18-45) years. The average time from injury until surgery was 14 (6-48) months. Preoperative and postoperative clinical and radiological parameters were evaluated. Mean follow-up was 16.8 (12-36) months. Results. Consolidation was achieved in all cases at 7 (4-10 weeks), no patient had complications or reoperations. Range of motion, pain, functional assessment (DASH questionnaire) and radiological measurements improved compared to preoperative measurements. The average range of flexion improved: flexion 71.9° (55°-80°) to 81.7° (55°-90°), extension 66.3° (30°-80°) to 84.4° (70° -90°), ulnar deviation 21.5 (10°-25°) to 25.5° (20°-45°) and radial deviation 11.9° (5°-25°) to 13.3° (10th-20th). Pain (VAS 0-10) improved from 6.8 (4-10) to 0.7 (0-3). DASH functional scale improved from 36 (12-78) to 8 (0-10). The Scapho-Lunate Angle improved from 67.7° (62°-88°) to 47° (32°-55°), and the Radio-Lunate Angle improved from 30.8° (10° -45°) to 4(0°-10°). Conclusion. Treatment of unstable scaphoid non-union with cancellous bone graft assisted by arthroscopy presents good clinical results with a short period of consolidation and recovery (AU)


Assuntos
Humanos , Adolescente , Adulto Jovem , Adulto , Pessoa de Meia-Idade , Osso Escafoide/cirurgia , Osso Escafoide , Pseudoartrose/cirurgia , Artroscopia/instrumentação , Transplante Autólogo/instrumentação , Pseudoartrose , Inquéritos e Questionários , Estudos Retrospectivos
9.
Rev Esp Cir Ortop Traumatol ; 61(4): 216-223, 2017.
Artigo em Inglês, Espanhol | MEDLINE | ID: mdl-28473231

RESUMO

OBJECTIVE: To evaluate the results of arthroscopic reconstruction for the treatment of unstable scaphoid non-union with cancellous bone autograft. METHODS: 13 patients were treated with a mean age of 26 (18-45) years. The average time from injury until surgery was 14 (6-48) months. Preoperative and postoperative clinical and radiological parameters were evaluated. Mean follow-up was 16.8 (12-36) months. RESULTS: Consolidation was achieved in all cases at 7 (4-10 weeks), no patient had complications or reoperations. Range of motion, pain, functional assessment (DASH questionnaire) and radiological measurements improved compared to preoperative measurements. The average range of flexion improved: flexion 71.9° (55°-80°) to 81.7° (55°-90°), extension 66.3° (30°-80°) to 84.4° (70° -90°), ulnar deviation 21.5 (10°-25°) to 25.5° (20°-45°) and radial deviation 11.9° (5°-25°) to 13.3° (10th-20th). Pain (VAS 0-10) improved from 6.8 (4-10) to 0.7 (0-3). DASH functional scale improved from 36 (12-78) to 8 (0-10). The Scapho-Lunate Angle improved from 67.7° (62°-88°) to 47° (32°-55°), and the Radio-Lunate Angle improved from 30.8° (10° -45°) to 4(0°-10°). CONCLUSION: Treatment of unstable scaphoid non-union with cancellous bone graft assisted by arthroscopy presents good clinical results with a short period of consolidation and recovery.


Assuntos
Artroscopia/métodos , Transplante Ósseo/métodos , Osso Esponjoso/transplante , Fixação de Fratura/métodos , Pseudoartrose/cirurgia , Osso Escafoide/lesões , Adolescente , Adulto , Feminino , Seguimentos , Humanos , Masculino , Pessoa de Meia-Idade , Estudos Retrospectivos , Osso Escafoide/cirurgia , Transplante Autólogo/métodos , Resultado do Tratamento , Adulto Jovem
10.
Kardiologiia ; 57(2): 17-22, 2017 Feb.
Artigo em Russo | MEDLINE | ID: mdl-28290785

RESUMO

High risk of macrovascular complications in patients with type 2 diabetes mellitus (T2DM) is caused by insulin resistance and atherogenic dyslipidemia that may be genetically determined. The aim of this study was to assess the association of polymorphic genetic variants APOA5 (S19W/rs3135506), CETP (Taq1B/rs708272), PON1 (Q192R /rs662) and PPARG (Pro12Ala /rs1801282) with T2DM and macrovascular complications in patients with T2DM resident in Northwestern Russia. We examined 386 patients with T2DM and 199 healthy controls. Genotyping was performed by polymerase chain reaction followed by restriction analysis. The study revealed the protective role of allele 12Ala of PPARG gene against T2DM development (odds ratio [OR]=0.58; 95% confidence interval [CI] 0.39-0.85). B1B1 genotype of CETP was associated with increased risk of stroke in T2DM patients (OR=1.85; 95%CI1.07-3.21). RR genotype of PON1 was associated with increased risk of T2DM with stroke (OR=2.98; 95%CI1.01-8.84). According to study results Pro12Ala (rs1801282) variant of PPARG affected the risk of T2DM; polymorphic variants of CETP (Taq1B/rs708272) and PON1 (Q192R/rs662) contributed to the risk of macrovascular complications of T2DM.


Assuntos
Diabetes Mellitus Tipo 2/complicações , Diabetes Mellitus Tipo 2/genética , Angiopatias Diabéticas/genética , Predisposição Genética para Doença , Arildialquilfosfatase , Humanos , Resistência à Insulina/genética , Polimorfismo Genético , Fatores de Risco , Federação Russa
11.
Tsitologiia ; 59(1): 27-33, 2017.
Artigo em Inglês, Russo | MEDLINE | ID: mdl-30188100

RESUMO

The adipose tissue is considered today as an endocrine organ in which tissue-specific regulation of gene expression plays a key role in the processes of development of obesity and comorbidities, such as diabetes and cardiovascular disease. The present study is focused on ITLN1, PPARã and TNFá gene expression in intra-abdominal adipose tissue and its effect on the serum levels of omentin 1 and TNFa in individuals with different body mass. It has been shown that serum TNFa level is significantly higher in the subgroup of patients with overweight and obesity (BMI ≥ 25 kg/m2) as compared to individuals with normal body weight (BMI < 25 kg/m2)( p < 0.03). We have demonstrated that the expression level of the PPARã gene is positively correlated with the ITLN1 gene expression level in the intra-abdominal adipose tissue (r = 0.516, p = 0.020). Serum level of omentin 1 positively correlates with PPARã mRNA and protein levels in intra-abdominal adipose tissue (r = 0.550, p < 0.05 and r = 0.581, p < 0.03, respectively). For the subgroup of patients with overweight and obesity, we have shown negative correlation of the level of TNFá mRNA with PPARã and ITLN1 mRNA levels was shown (r = ­0.549, p < 0.05 and r = ­0.475, p < 0.05, respectively). This study is the first to show a correlation relationship between PPARã gene expression level in the intra-abdominal adipose tissue and the expression and secretion levels of omentin 1.


Assuntos
Citocinas/biossíntese , Regulação da Expressão Gênica , Gordura Intra-Abdominal/metabolismo , Lectinas/biossíntese , Obesidade/metabolismo , PPAR gama/biossíntese , Fator de Necrose Tumoral alfa/biossíntese , Adulto , Feminino , Proteínas Ligadas por GPI/biossíntese , Humanos , Gordura Intra-Abdominal/patologia , Masculino , Pessoa de Meia-Idade , Obesidade/patologia
12.
Neurosci Lett ; 636: 70-76, 2017 01 01.
Artigo em Inglês | MEDLINE | ID: mdl-27780739

RESUMO

Alpha-synuclein oligomerization plays a key role in the development of Parkinson's disease (PD). Being the most common genetic contributor to PD, glucocerebrosidase 1 (GBA) mutations have been associated with decreased GBA enzymatic activity in PD patients with mutations in the GBA gene (GBA-PD). However, it is unknown whether the activities of other lysosomal hydrolases are being altered in GBA-PD patients and are accompanied by an increase in alpha-synuclein oligomerization. The aim of our study was to estimate GBA enzymatic activity as well as the activities of five other lysosomal hydrolases (galactocerebrosidase, alpha-glucosidase, alpha-galactosidase, sphingomyelinase, alpha-iduronidase) in dried blood spots with assessing plasma oligomeric alpha-synuclein levels in sporadic PD (sPD) patients, in GBA-PD patients and in controls. GBA enzymatic activity and plasma oligomeric alpha-synuclein levels were assessed in sPD patients (N=84), in GBA-PD patients (N=21) and controls (N=62) by LC-MS/MS and ELISA methods accordingly. GBA-PD patients showed lower GBA enzymatic activity compared to controls (p=0.001) and to sPD (p=0.0001). We also found the reduction of GLA enzymatic activity (but not of other lysosomal hydrolases) in GBA-PD (p=0.001). At the same time plasma oligomeric alpha-synuclein levels were increased in GBA-PD group compared to sPD and controls (p=0.002 and p<0.0001, respectively). Our results suggest that the decrease in enzymatic activity of lysosomal hydrolases in GBA mutation carriers may contribute to PD pathogenesis by increasing the level of neurotoxic oligomeric alpha-synuclein species.


Assuntos
Glucosilceramidase/metabolismo , Doença de Parkinson/metabolismo , alfa-Sinucleína/sangue , Idoso , Estudos de Casos e Controles , Feminino , Glucosilceramidase/genética , Humanos , Masculino , Pessoa de Meia-Idade , Mutação , Doença de Parkinson/genética , Agregados Proteicos
13.
Artigo em Russo | MEDLINE | ID: mdl-27635612

RESUMO

Mutations in the GBA and SMPD1 genes, which lead to the development of lysosomal storage diseases, are high risk factors for Parkinson's disease and dementia with Lewy bodies. We screened the mutations in the GALC and CLN3 genes in patients with Parkinson's disease and control subjects. A heterozygous CLN3 mutation (del 1.02 kb) carrier with clinical features of the unusual extrapyramidal syndrome was identified. A role of CLN3 mutations in the development of neurodegenerative disorders is discussed.


Assuntos
Doenças dos Gânglios da Base/genética , Glicoproteínas de Membrana/genética , Chaperonas Moleculares/genética , Deleção de Sequência , Idoso , Feminino , Testes Genéticos , Heterozigoto , Humanos , Leucodistrofia de Células Globoides/genética , Masculino , Doença de Parkinson/genética , Linhagem , Síndrome
14.
Biomed Khim ; 62(3): 283-9, 2016 Mar.
Artigo em Russo | MEDLINE | ID: mdl-27420620

RESUMO

Tissue specific expression of genes encoding cholesterol transporters ABCA1 and ABCG1 as well as genes encoding the most important transcriptional regulators of adipogenesis - LXRa, LXRb, PPARg and RORa has been investigated in intraabdominal adipose tissue (IAT) samples.A direct correlation between the content of ABCA1 and ABCG1 proteins with RORa protein level (r=0.480, p<0.05; r=0.435, p<0.05, respectively) suggests the role of the transcription factor RORa in the regulation of IAT ABCA1 and ABCG1 protein levels. ABCA1 and ABCG1 gene expression positively correlated with obesity indicators such as body mass index (BMI) (r=0.522, p=0.004; r=0.594, p=0.001, respectively) and waist circumference (r=0.403, p=0.033; r=0.474, p=0.013, respectively). The development of obesity is associated with decreased IAT levels of RORa and LXRb mRNA (p=0.016 and p=0.002, respectively). These data suggest that the nuclear factor RORa can play a significant role in the regulation of cholesterol metabolism and control IAT expression of ABCA1 and ABCG1, while the level of IAT LXRb gene expression may be an important factor associated with the development of obesity.


Assuntos
Transportador 1 de Cassete de Ligação de ATP/metabolismo , Membro 1 da Subfamília G de Transportadores de Cassetes de Ligação de ATP/metabolismo , Gordura Abdominal/metabolismo , Obesidade/metabolismo , Transportador 1 de Cassete de Ligação de ATP/genética , Membro 1 da Subfamília G de Transportadores de Cassetes de Ligação de ATP/genética , Adulto , Composição Corporal , Estudos de Casos e Controles , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Membro 1 do Grupo F da Subfamília 1 de Receptores Nucleares/genética , Membro 1 do Grupo F da Subfamília 1 de Receptores Nucleares/metabolismo , Obesidade/genética
15.
Tsitologiia ; 58(2): 99-104, 2016.
Artigo em Russo | MEDLINE | ID: mdl-27228655

RESUMO

Impaired metabolism of alpha-synuclein (SNCA) and its aggregation are now implicated in the pathogenesis of Parkinson's disease (PD). Previous studies have found association between PD and gene locus, containing the SNCA gene. Meta-analysis have shown high significant association of single nucleotide polymorphisms (SNPs) rs356165 (A/G) and rs356219 (A/G) in the SNCA gene with PD. We genotyped these SNPs in 260 PD patients and 262 controls from north-western region of Russia. Alleles "G" of rs356165 and rs356219 were associated with increased risk of PD development. Linkage disequilibrium was shown between associated marker alleles. We studied the relationship between rs356165 and rs356219 and levels of mRNA SNCA and alpha-synuclein in CD45+ peripheral blood cells in drug-naive PD patients (n = 43) and controls (n = 39). Alleles "G" of rs356165 and rs356219 were associated with increased levels of SNCA expression (p = 0.046) and high alpha-synuclein levels (p = 0.039) in controls. Our data suggest that rs356165 and rs356219 variants might influence on PD development by upregulating SNCA expression.


Assuntos
Estudos de Associação Genética , Doença de Parkinson/genética , RNA Mensageiro/genética , alfa-Sinucleína/genética , Idoso , Células Sanguíneas , Feminino , Genótipo , Humanos , Antígenos Comuns de Leucócito/genética , Masculino , Pessoa de Meia-Idade , Doença de Parkinson/patologia , Polimorfismo de Nucleotídeo Único , RNA Mensageiro/biossíntese , Ativação Transcricional , alfa-Sinucleína/biossíntese
16.
Biochemistry (Mosc) ; 80(11): 1522-7, 2015 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-26615445

RESUMO

Stability of composition and sequence of genes was shown earlier in 13 mitochondrial genomes of mosses (Rensing, S. A., et al. (2008) Science, 319, 64-69). It is of interest to study the evolution of mitochondrial genomes not only at the gene level, but also on the level of nucleotide sequences. To do this, we have constructed a "nucleotide pangenome" for mitochondrial genomes of 24 moss species. The nucleotide pangenome is a set of aligned nucleotide sequences of orthologous genome fragments covering the totality of all genomes. The nucleotide pangenome was constructed using specially developed new software, NPG-explorer (NPGe). The stable part of the mitochondrial genome (232 stable blocks) is shown to be, on average, 45% of its length. In the joint alignment of stable blocks, 82% of positions are conserved. The phylogenetic tree constructed with the NPGe program is in good correlation with other phylogenetic reconstructions. With the NPGe program, 30 blocks have been identified with repeats no shorter than 50 bp. The maximal length of a block with repeats is 140 bp. Duplications in the mitochondrial genomes of mosses are rare. On average, the genome contains about 500 bp in large duplications. The total length of insertions and deletions was determined in each genome. The losses and gains of DNA regions are rather active in mitochondrial genomes of mosses, and such rearrangements presumably can be used as additional markers in the reconstruction of phylogeny.


Assuntos
Briófitas/genética , DNA Mitocondrial/metabolismo , Filogenia , Briófitas/classificação , Bases de Dados Genéticas , Internet , Interface Usuário-Computador
17.
Neurosci Lett ; 583: 188-93, 2014 Nov 07.
Artigo em Inglês | MEDLINE | ID: mdl-25265039

RESUMO

A link between lysosomal storage diseases (LSDs) and neurodegenerative disorders associated with accumulation of presynaptic protein alpha-synuclein has been shown. Particularly, Gaucher disease (GD) patients with a deficiency of the lysosomal enzyme glucocerebrosidase (GBA) and carriers of GBA mutations are at increased risk of Parkinson's disease (PD). It remains unclear whether this link is due to increased alpha-synuclein oligomerization. Here we show that level of oligomeric alpha-synuclein form, associated with PD development, is increased in plasma of GD patients (n=41, median=22.9pg/mL, range1.57-444.58pg/mL; controls (n=40, median=6.02pg/mL, range 1.05-103.14pg/mL, p<0.0001). This difference is absent in GD patients receiving enzyme replacement therapy (ERT) for more than 5 years. Moreover, the levels of alpha-synuclein oligomers in plasma are also higher in patients with other LSDs (Niemann-Pick type C, Krabbe disease, Wolman disease) compared to the median value in controls. Therefore, we suggest that mutations in the GBA gene and at least in several other LSDs genes may be associated with an increase in oligomeric alpha-synuclein in plasma. ERT applied for recovering of GBA functions in GD treatment might decrease formation of plasma oligomeric alpha-synuclein.


Assuntos
Doenças por Armazenamento dos Lisossomos/sangue , alfa-Sinucleína/sangue , Adolescente , Adulto , Fatores Etários , Idoso , Estudos de Casos e Controles , Criança , Pré-Escolar , Terapia de Reposição de Enzimas , Feminino , Humanos , Lactente , Doenças por Armazenamento dos Lisossomos/tratamento farmacológico , Masculino , Pessoa de Meia-Idade , Adulto Jovem
19.
Artigo em Russo | MEDLINE | ID: mdl-20218340

RESUMO

AIM: Genotyping of Hib strains isolated in regions of Russia as well as characterization of genetic relations of typed strains with strains isolated in other areas. MATERIALS AND METHODS: Genetic characterization of 31 strains of Hib isolated in Russian regions during 2005-2008 was performed by multilocus sequence typing. RESULTS: Studied strains belonged to 11 variants of sequence types, 6 of which were described in previous studies, whereas other 5 were isolated for the first time during this study. The most common isolated strains were ST-92 (13 strains or 42%) and ST-6 (6 strains or 19%). Typed strains were distributed to two clonal complexes. Clonal complex "A1/A2" ("ST-6") incorporates all typed strains except ST-93 strain belonging to clonal complex "B1b" ("ST-93"). The majority of studied strains (19 or 61%) had difference from "central" sequence type of clonal complex, A1/A2 ("ST-6") on not more than one allele. CONCLUSION: Clonal structure of isolated strains is analogous to the one observed in Moscow and foreign strains.


Assuntos
Infecções por Haemophilus/epidemiologia , Haemophilus influenzae tipo b/classificação , Técnicas de Tipagem Bacteriana , Genes Bacterianos/genética , Haemophilus influenzae tipo b/genética , Humanos , Epidemiologia Molecular , Federação Russa/epidemiologia , Análise de Sequência
20.
Gig Sanit ; (2): 8-12, 2006.
Artigo em Russo | MEDLINE | ID: mdl-16758810

RESUMO

The paper considers the significance of complex entrance of chloroform from portable water into the human body (enterally, inhalationally, and through the intact skin). It shows it necessary to toughen the maximum permissible concentration (MPC) of chloroform in the portable drinking, by taking into account of the multiplicity of routes of its action on the population. The authors present the results of their own investigations of the levels of chloroform in the air of bath and shower rooms before and after taking a shower and filling the bath with water, as well as in the airspace layer above the water of an indoor swimming pool, by using chromatographic mass-spectrometry.


Assuntos
Clorofórmio/efeitos adversos , Indicadores Básicos de Saúde , Solventes/efeitos adversos , Água/normas , Carcinógenos/toxicidade , Humanos , Federação Russa
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