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1.
Nan Fang Yi Ke Da Xue Xue Bao ; 41(8): 1226-1233, 2021 Aug 20.
Artigo em Chinês | MEDLINE | ID: mdl-34549715

RESUMO

OBJECTIVE: We propose an efficient method to reduce the noise in low-dose cerebral perfusion CT images using prior image constrained diffusion tensor to reduce the radiation dose in brain CT examination. METHODS: By utilizing the redundant information in cerebral perfusion CT images, we embedded the complementary structure information in prior images into lowdose cerebral perfusion CT image restoration process to suppress the image noise and artifacts.We first calculated the diffusion tensor for the low-dose cerebral perfusion CT image and prior image separately and then constructed a prior image constrained diffusion tensor (PICDT) to incorporate the structure information from the prior image into low-dose image restoration process. RESULTS: In experiments with the Shepp-Logan phantom, the SSIM value of CBF map obtained by the proposed algorithm was increased by 63% as compared with that of the FBP algorithm.In analysis of the clinical dataset, the SSIM value of CBF map obtained by the proposed algorithm was increased by 45% as compared with that of FBP algorithm. CONCLUSION: The proposed method can effectively reduce noises and artifacts of low-dose cerebral perfusion CT images while maintaining the structural details to obtain accurate cerebral hemodynamic maps.


Assuntos
Algoritmos , Tomografia Computadorizada por Raios X , Artefatos , Circulação Cerebrovascular , Processamento de Imagem Assistida por Computador , Imagens de Fantasmas , Doses de Radiação
2.
Zhonghua Wai Ke Za Zhi ; 59(9): 799-803, 2021 Sep 01.
Artigo em Chinês | MEDLINE | ID: mdl-34404180

RESUMO

In the past,treatment of acute ilio-femoral deep vein thrombosis (IFDVT) was mainly based on anticoagulation alone,but 30%-50% of patients will develop post-thrombotic syndrome,causing a serious medical burden.Thrombus removal technology such as catheter-directed thrombolysis and percutaneous mechanical thrombectomy can effectively remove blood clots and compensate for the deficiencies of simple anticoagulation,which is expected to improve the prognosis of such disease,but the current evidence is insufficient,and other treatments such as filter implantation and compression therapy are also controversial.This article summarizes the treatment strategies and the latest progress of acute IFDVT,hoping to help the treatment of this type of disease.

3.
Eur Rev Med Pharmacol Sci ; 25(8): 3173-3181, 2021 04.
Artigo em Inglês | MEDLINE | ID: mdl-33928603

RESUMO

OBJECTIVE: To evaluate the effectiveness of case-based learning (CBL) in medical students' education through meta-analysis. MATERIALS AND METHODS: PubMed, Cochrane Library, Elsevier and other databases were searched to find randomized controlled trials (RCTs) of CBL teaching methods and other teaching methods published from January 1, 1995, to October 1, 2020. All included studies used the Cochrane risk bias assessment tool, and Review Manager software, version 5.3 (Copenhagen, Denmark), was used for the meta-analysis and systematic review. RESULTS: A total of 8 studies were included with a total of 939 students, including 480 in the CBL group and 459 in the control group. Compared with other teaching methods, CBL teaching can improve medical students' academic performance (p=0.03) and case analysis ability (p<0.001). CONCLUSIONS: CBL is an active teaching method that is effective for educating medical students and helps to improve their performance and case analysis ability.

4.
Res Vet Sci ; 135: 343-348, 2021 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-33129574

RESUMO

Tibial dyschondroplasia (TD) is an intractable avian cartilage disease in which proximal growth plates of tibia lack blood vessels and contain nonviable cells, and it leads to the inflammatory response. Prostaglandins (PGs) genes have not been studied yet in TD chicken, and they might play role in skeletal metabolism, therefore we planned to explore the role of recombinant glutathione-S-transferase A3 (rGSTA3) protein and PG-related genes. In this study, qRT-PCR, enzyme-linked immunosorbent assay (ELISA) and immunohistochemistry (IHC) analysis were used to identify the expression patterns of eight PG-related genes in the tibial growth plate of broiler chicken. The results showed that the expression of PG-related genes glutathione-S-transferase A3 (GSTA3), cyclooxygenase 2 (COX-2), prostaglandin D2 synthase (PTGDS), prostaglandin E synthase (PTGES), prostaglandin E2 receptor (PTGER) 3, PTGER4, prostaglandin reductase 1 (PTGR1) and hematopoietic prostaglandin D synthases (HPGDS) expression were identified and could significantly respond to thiram-induced TD chicken. Interestingly, the expression of rate-limiting enzyme COX-2 and PGE2 were induced after the treatment of rGSTA3 protein. These findings demonstrated that the occurrence of TD is closely related to the inhibition of PGs. Moreover, rGSTA3 protein participated in the recovery of TD by strengthening the expression of PG-related genes.


Assuntos
Galinhas , Glutationa Transferase/farmacologia , Osteocondrodisplasias/veterinária , Doenças das Aves Domésticas/prevenção & controle , Tíbia/efeitos dos fármacos , Animais , Regulação Enzimológica da Expressão Gênica/efeitos dos fármacos , Masculino , Osteocondrodisplasias/tratamento farmacológico , Proteínas Recombinantes/farmacologia , Tiram/efeitos adversos , Tíbia/crescimento & desenvolvimento , Tíbia/patologia
5.
Zhonghua Zhong Liu Za Zhi ; 42(5): 391-395, 2020 May 23.
Artigo em Chinês | MEDLINE | ID: mdl-32482028

RESUMO

Objective: To investigate the expression of IGF1R-Ras and RAGE-HMGB1 signaling pathways in colorectal cancer patients with type 2 diabetes mellitus and their significance. Methods: The resected cancer tissues were obtained from 59 patients with colorectal cancer (CRC), including 29 patients with type 2 diabetes mellitus (CRC/DM group) and 30 with CRC alone (CRC group). The expressions of IGF1R, Ras, RAGE and HMGB1 in cancer tissues were detected by immunohistochemistry. The differences between the two groups were compared and the relationship between the expression and clinicopathological characteristics was analyzed. Results: In CRC/DM group, the positive rates of IGF1R and Ras were both 65.5% (19/29), and 51.7% (15/29) patients had IGF1R+ Ras+ immunophenotype, which were significantly higher than those in CRC group [33.3% (10/30), 36.7% (11/30) and 20.0% (6/30); P=0.013, 0.027 and 0.011, respectively]. The expression of IGF1R and Ras in CRC / DM group was positively correlated (r=0.479, P=0.017). The positive rate of RAGE expression in CRC group and CRC/DM group was 70.0% (21/30) and 72.4% (21/29) respectively, and the positive rate of HMGB1 expression was 46.7% (14/30) and 58.6% (17/29) respectively, neither was observed with significant difference (P=0.358 and 0.838). However, the proportion of patients with RAGE+ HMGB1+ immunophenotype in CRC/DM group [55.2% (16/29)] was higher than that in CRC Group [26.7% (8/30)] which was statistically significant (P=0.026), and the expression of both proteins was positively correlated in CRC/DM group (r=0.578, P=0.003). The clinicopathological analysis showed that in both groups the expression of IGF1R, Ras, RAGE and HMGB1 had no correlation with the sex, age, differentiation degree, tumor length, T stage and lymph node metastasis (P>0.05). Conclusion: Both IGF1R-Ras and RAGE-HMGB1 pathways may be involved in the oncogenesis of colorectal cancer in patients with type 2 diabetes.


Assuntos
Neoplasias Colorretais/patologia , Diabetes Mellitus Tipo 2/complicações , Genes ras/genética , Proteína HMGB1/metabolismo , Receptor IGF Tipo 1/metabolismo , Biomarcadores Tumorais/genética , Biomarcadores Tumorais/metabolismo , Neoplasias Colorretais/complicações , Neoplasias Colorretais/metabolismo , Proteína HMGB1/genética , Humanos , Prognóstico , Receptor IGF Tipo 1/genética
6.
Eur Rev Med Pharmacol Sci ; 24(6): 3411-3421, 2020 03.
Artigo em Inglês | MEDLINE | ID: mdl-32271460

RESUMO

OBJECTIVE: On December 8, 2019, many cases of pneumonia with unknown etiology were first reported in Wuhan, China, subsequently identified as a novel coronavirus infection aroused worldwide concern. As the outbreak is ongoing, more and more researchers focused interest on the COVID-19. Therefore, we retrospectively analyzed the publications about COVID-19 to summarize the research hotspots and make a review, to provide reference for researchers in the world. MATERIALS AND METHODS: We conducted a search in PubMed using the keywords "COVID-19" from inception to March 1, 2020. Identified and analyzed the data included title, corresponding author, language, publication time, publication type, research focus. RESULTS: 183 publications published from 2020 January 14 to 2020 February 29 were included in the study. The first corresponding authors of the publications were from 20 different countries. Among them, 78 (42.6%) from the hospital, 64 (35%) from the university and 39 (21.3%) from the research institution. All the publications were published in 80 different journals. Journal of Medical Virology published most of them (n=25). 60 (32.8%) were original research, 29 (15.8%) were review, 20 (10.9%) were short communications. 68 (37.2%) epidemiology, 49 (26.8%) virology and 26 (14.2%) clinical features. CONCLUSIONS: According to our review, China has provided a large number of research data for various research fields, during the outbreak of COVID-19. Most of the findings play an important role in preventing and controlling the epidemic around the world. With research on the COVID-19 still booming, new vaccine and effective medicine for COVID-19 will be expected to come out in the near future with the joint efforts of researchers worldwide.


Assuntos
Bibliometria , Infecções por Coronavirus , Pandemias , Pneumonia Viral , Betacoronavirus , COVID-19 , Teste para COVID-19 , Técnicas de Laboratório Clínico , Infecções por Coronavirus/diagnóstico , Infecções por Coronavirus/epidemiologia , Infecções por Coronavirus/virologia , Surtos de Doenças , Humanos , Pneumonia Viral/diagnóstico , Pneumonia Viral/epidemiologia , Pneumonia Viral/virologia , SARS-CoV-2
7.
ISA Trans ; 102: 245-250, 2020 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-32122638

RESUMO

Tip-Tilt mirrors play an important role in astronomical telescopes requiring the tracking performance at the level of microradian or sub-microradian. However, the closed-loop performance suffers a lot from the low-sample rate and time delay of image sensors. Especially, this issue is under the condition of vibrations, because dynamic behaviors are complex and the models are difficult to be obtained accurately. Another challenging issue comes from the measurement of vibrations and its extraction for the closed-loop control. This paper proposes a new method based on an add-on controller of the Tip-Tilt mirror to mitigate telescope vibrations. The proposed method only uses Tip-Tilt errors from an image sensor to implement a disturbance observer, which is not being restricted by an accurate model. As a result, the closed-loop performance can be optimized by designing of a proper Q-filter. To suppress the low-frequency and high-frequency vibrations, a novel Q-filter combining a lowpass filter and a bandpass filter is proposed here. The improved control method is validated by both simulation and experiment in the tip-tilt mirror control system under the condition of vibrations.

8.
Zhonghua Kou Qiang Yi Xue Za Zhi ; 55(2): 98-103, 2020 Feb 09.
Artigo em Chinês | MEDLINE | ID: mdl-32074670

RESUMO

Objective: To analyze the correlation between the age and the cone-beam CT (CBCT) images of the third and fourth cervical vertebrae in female skeletal class Ⅰ patients aged between 9 and 17 years, and to establish a quantitative evaluation method for calculating the age. Methods: CBCT images of 108 female skeletal class Ⅰ patients aged between 9 and 17 years were collected from Qingdao Stomatological Hospital from September, 2017 to March, 2019. The two-dimensional linear values (AH: height of anterior edge of vertebral body; H: height of middle part of vertebral body; PH: height of posterior edge of vertebral body; AP: width of vertebral body), the two-dimensional linear ratio values (AH/PH, AH/AP, AH/H, H/AP, H/PH, PH/AP) and the three-dimensional volume values of the third vertical vertebrae (C3) and the fourth vertical vertebrae (C4) were measured. By Exponential transformation of measurements and multiple linear regression analysis, the optimal index for evaluating age were screened, and the fitting degree of multiple linear regression equation (R(2)) and the accuracy of age estimation (SEE) were compared. CBCT images of 27 female skeletal class Ⅰ patients aged from 9 to 17 years were added from Qingdao Stomatological Hospital between April, 2019 and July, 2019, by which the accuracy of the regression equation was verified. Results: Multiple linear regression equation for age estimation based on two-dimensional linear indexes was as follows: Y=-113.928+33.743×e(AH)(3)(/100)+58.844×e(PH)(4)(/100)+20.590×e(AP)(4)(/100)( "e" was a natural constant, e≈2.718), R(2)=0.745, SEE=1.31. Multiple linear regression equation for age estimation based on two-dimensional linear ratio indexes was as follows: Y=-0.076-2.284×e(A)H(3)/PH(3)+3.227×e(A)H(3)/AP(3)+2.149×e(A)H(3)/H(3)+1.961×e(A)H(4)/H(4), R(2)=0.576, SEE=1.70. Multiple linear regression equation of age estimation by the volume index was as follows: Y=-16.828+22.184×e(V)(3)(/10 000), R(2)=0.555, SEE=1.71. The data of 27 new patients were tested. The CBCT measurement index of C3 and C4 vertebral bodies inferred the fitting degree (R(2)) and accuracy (SEE) of the equation of the age estimation. The two-dimensional linear value was superior to the two-dimensional linear ratio and the latter was superior to the three-dimensional volume value. The standard error of the estimate about them was 1.74, 2.00 and 2.37, respectively. Conclusions: The two-dimensional linear index of CBCT images of C3 and C4 could be used to estimate the age of 9 to 17-year-old female skeletal class Ⅰ patients, and the accuracy of the method was higher than that of two-dimensional ratio index and three-dimensional volume index.


Assuntos
Vértebras Cervicais/diagnóstico por imagem , Tomografia Computadorizada de Feixe Cônico , Adolescente , Criança , Feminino , Humanos , Análise Multivariada , Análise de Regressão
9.
Res Vet Sci ; 124: 112-117, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-30878632

RESUMO

Tibial dyschondroplasia (TD) is a type of bone deformity found in fast-growing chickens, which induce inflammatory responses. Prostaglandins (PGs) implicate in bone formation and bone resorption, associated with inflammation in an autocrine/paracrine manner. This study used qRT-PCR and immunohistochemistry analysis to identify the expression patterns of PG-related genes in the erythrocytes of broiler chickens and explore the effects of thiram-induced TD and the recombinant glutathione-S-transferase A3 (rGSTA3) protein on the expression of PG-related genes: GSTA3, cyclooxygenase 2 (COX-2), prostaglandin D2 synthase (PTGDS), prostaglandin E synthase (PTGES), prostaglandin E2 receptor (PTGER) 3, PTGER4 and prostaglandin reductase 1 (PTGR1). Interestingly, the results showed that these seven PG-related genes expression was identified in the erythrocytes of broiler chicken, and thiram-induced TD suppressed the expression of these PG-related genes in the initial stage of TD and promoted their expression in TD recovery. These findings demonstrated that the immunoregulatory function of erythrocytes can be inhibited in the early stage of TD and promoted in the recovery stage by modulating the expression of PG-related genes. Further, the rGSTA3 protein can modulate the expression of PG-related genes in erythrocytes and participate in the recovery of TD.


Assuntos
Galinhas , Glutationa Transferase/farmacologia , Osteocondrodisplasias/veterinária , Doenças das Aves Domésticas/genética , Prostaglandinas/genética , Tíbia/patologia , Animais , Proteínas Aviárias/farmacologia , Eritrócitos/metabolismo , Mutagênicos/farmacologia , Osteocondrodisplasias/induzido quimicamente , Osteocondrodisplasias/genética , Doenças das Aves Domésticas/induzido quimicamente , Prostaglandinas/metabolismo , Proteínas Recombinantes/farmacologia , Tiram/farmacologia
10.
Res Vet Sci ; 120: 11-16, 2018 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-30165245

RESUMO

Thiram, a carbamate pesticide, is known to induce tibial dyschondroplasia (TD) in broiler chickens. This study used a thiram-induced TD model to explore whether apoptosis-related genes were expressed in erythrocytes of broiler chickens and the impacts of thiram-induced TD and the recombinant GSTA3 protein in regulating these genes expression. In this study, mRNA and protein expression of six types of apoptosis-related genes (Bcl-2, Bax, Murine double minute MDM2, Bcl-2-associated athanogene BAG-1, BAG-3, STAT3) were identified in erythrocytes of broiler chickens by real-time PCR and immunohistochemistry, and we also found that thiram-induced TD induced the decreased expression of these antiapoptotic genes in the initial stage of TD and promoted their expression in TD recovery, which suggested that the expression of these apoptosis-related genes in erythrocytes is highly related to the development of TD. Further, the recombinant GSTA3 protein promoted the expression of all apoptosis-related genes in the initial stage of TD and recovered the normal expression of these genes in the recovery stage of TD, which indicated that the recombinant GSTA3 protein may participate in the recovery of TD. Further studies are needed to elucidate the mechanism of the response of erythrocytes to thiram-induced TD and the recombinant protein GSTA3 in broiler chickens.


Assuntos
Apoptose/fisiologia , Glutationa Transferase/farmacologia , Osteocondrodisplasias/veterinária , Doenças das Aves Domésticas/genética , Tiram/toxicidade , Animais , Apoptose/genética , Galinhas/genética , Eritrócitos/metabolismo , Expressão Gênica/efeitos dos fármacos , Regulação da Expressão Gênica , Predisposição Genética para Doença , Glutationa/metabolismo , Osteocondrodisplasias/genética , Doenças das Aves Domésticas/metabolismo , RNA Mensageiro/genética , Proteínas Recombinantes/farmacologia , Transferases
11.
Water Sci Technol ; 2017(3): 859-868, 2018 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-30016303

RESUMO

A new carbonized pomelo peel biosorbent (MCPP) modified with nanoscale zero-valent iron (NZVI) and cetyl-trimethyl ammonium bromide was prepared and employed for the adsorption of molybdate (Mo(VI)) from aqueous solution. We investigated the effects of various conditions on Mo(VI) adsorption and evaluated the results based on adsorption kinetics models and isotherm equations. The kinetic data fitted to the pseudo-second-order model. The Langmuir model best described the adsorption of Mo(VI) on MCPP. The values of changes in Gibbs free energy, standard enthalpy, and standard entropy revealed that the adsorption process was feasible, spontaneous and endothermal. X-ray diffraction, Fourier transform infrared and X-ray photoelectron spectroscopy measurements suggested that Mo(VI) adsorption occurred via both the reduction and surface adsorption. Thus, biochar, prepared from fruit residue, can be applied to remove Mo(VI) from aqueous solutions. More importantly, our results provide a sustainable approach for Mo(VI) removal from wastewater by means of functional modification.


Assuntos
Brometos/química , Carvão Vegetal , Ferro/química , Nanopartículas Metálicas/química , Molibdênio/química , Compostos de Amônio Quaternário/química , Adsorção , Cetrimônio , Compostos de Cetrimônio , Cromo/química , Cinética , Soluções , Águas Residuárias/química , Poluentes Químicos da Água/química , Difração de Raios X
12.
Nature ; 560(7716): E1, 2018 08.
Artigo em Inglês | MEDLINE | ID: mdl-29875404

RESUMO

In this Brief Communications Arising Reply, the affiliation for author P. H. Templer was incorrectly listed as 'Department of Ecology & Evolutionary Biology, University of California Irvine, Irvine, California 92697, USA' instead of 'Department of Biology, Boston University, Boston, Massachusetts 02215, USA'. This has been corrected online.

14.
J Fish Biol ; 91(1): 368-374, 2017 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-28508492

RESUMO

Experiments on the swimming kinetics and behaviour of weather loach Misgurnus anguillicaudatus showed that horizontal swim speed was significantly greater than swim speeds when ascending to or descending from the water surface to gulp air. Vertical swimming speeds during ascending or descending were similar. Misgurnus anguillicaudatus swam unsteadily during vertical movements compared with horizontal movements.


Assuntos
Cipriniformes/fisiologia , Natação/fisiologia , Análise de Variância , Animais , Pesqueiros , Tempo (Meteorologia)
15.
Nature ; 540(7631): 104-108, 2016 11 30.
Artigo em Inglês | MEDLINE | ID: mdl-27905442

RESUMO

The majority of the Earth's terrestrial carbon is stored in the soil. If anthropogenic warming stimulates the loss of this carbon to the atmosphere, it could drive further planetary warming. Despite evidence that warming enhances carbon fluxes to and from the soil, the net global balance between these responses remains uncertain. Here we present a comprehensive analysis of warming-induced changes in soil carbon stocks by assembling data from 49 field experiments located across North America, Europe and Asia. We find that the effects of warming are contingent on the size of the initial soil carbon stock, with considerable losses occurring in high-latitude areas. By extrapolating this empirical relationship to the global scale, we provide estimates of soil carbon sensitivity to warming that may help to constrain Earth system model projections. Our empirical relationship suggests that global soil carbon stocks in the upper soil horizons will fall by 30 ± 30 petagrams of carbon to 203 ± 161 petagrams of carbon under one degree of warming, depending on the rate at which the effects of warming are realized. Under the conservative assumption that the response of soil carbon to warming occurs within a year, a business-as-usual climate scenario would drive the loss of 55 ± 50 petagrams of carbon from the upper soil horizons by 2050. This value is around 12-17 per cent of the expected anthropogenic emissions over this period. Despite the considerable uncertainty in our estimates, the direction of the global soil carbon response is consistent across all scenarios. This provides strong empirical support for the idea that rising temperatures will stimulate the net loss of soil carbon to the atmosphere, driving a positive land carbon-climate feedback that could accelerate climate change.


Assuntos
Atmosfera/química , Ciclo do Carbono , Carbono/análise , Geografia , Aquecimento Global , Solo/química , Bases de Dados Factuais , Ecossistema , Retroalimentação , Modelos Estatísticos , Reprodutibilidade dos Testes , Temperatura
16.
Genet Mol Res ; 15(4)2016 Nov 21.
Artigo em Inglês | MEDLINE | ID: mdl-27886349

RESUMO

Sillago sihama (Forsskål, 1775), a commercially important marine fishery species in the Indo-West Pacific, is being developed as a target species for aquaculture and stock enhancement in China. However, due to the limitations of traditional isolation methods, the available microsatellite loci, or simple sequence repeats (SSRs), of S. sihama lack diversity. We used a stepwise approach including Illumina sequencing, primer screening, and SSR marker validation to develop diverse SSRs for S. sihama. A total of 853.48 Mb clean sequences were assembled with high coverage and sequencing depth, and 27,288 potential SSRs were identified. A set of 18 novel SSR markers with four type motifs including 11 di-, 1 tri-, 5 tetra-, and 1 hexanucleotide repeats were successfully isolated. The ranges of number of alleles per locus and observed and expected heterozygosities were 5-24, 0.226-0.968, and 0.319-0.950, respectively. The diversity parameters exhibited high levels of polymorphism in these 18 loci. Three loci with the presence of both null alleles and inbreeding showed significant deviation from Hardy-Weinberg equilibrium after Bonferroni correction. Moreover, 13 loci developed in S. sihama showed high transferability to the closely related species Sillago japonica. The polymorphic SSR markers developed in this study may serve as valuable tools for further basic and applied research on the genetic resources of S. sihama as well as S. japonica. Our results indicate that this approach, based on next-generation sequencing technology, is convenient, cost-effective, and suitable for SSR marker isolation in other sillaginid fishes.


Assuntos
Sequenciamento de Nucleotídeos em Larga Escala/métodos , Repetições de Microssatélites , Perciformes/genética , Análise de Sequência de DNA/métodos , Animais , Aquicultura , China , Frequência do Gene , Marcadores Genéticos , Humanos , Perciformes/crescimento & desenvolvimento
17.
Genet Mol Res ; 15(3)2016 Sep 16.
Artigo em Inglês | MEDLINE | ID: mdl-27706718

RESUMO

DNA methylation is an important epigenetic modification in eukaryotes, which plays a significant role in regulating gene expression. When the host is invaded by the influenza virus, gene expression is regulated via changes in DNA methylation levels or patterns, leading to the activation or suppression of relevant signaling pathways or networks, triggering a series of immune responses against viral invasion. Here, we investigated the changes in genomic DNA methylation in the immune organs of chicken infected with H5N1 influenza virus. Genome-wide DNA methylation levels in the spleen, thymus, and bursa of Fabricius of specific pathogen-free (SPF) chicken infected with the Guangdong (G-H5N1) and Anhui (A-H5N1) H5N1 strains, and water (control) were analyzed by fluorescence-labeled methylation-sensitive amplified polymorphism (F-MSAP). The results indicated that total DNA methylation levels did not differ between spleen genomic DNA in chicken treated with different viral strains and the control (P > 0.05). However, the total DNA methylation levels were significantly upregulated in the thymus (P < 0.01) and bursa (P < 0.05) of chicken in the A-H5N1 group compared to those in the G-H5N1 and control groups. These results provide a basis for the screening of avian influenza-resistance genes or methylation markers, analyzing the epigenetic regulation mechanisms of avian influenza, and performing selective breeding for disease resistance.


Assuntos
Metilação de DNA/genética , Resistência à Doença/genética , Virus da Influenza A Subtipo H5N1/patogenicidade , Influenza Aviária/genética , Animais , Bolsa de Fabricius/imunologia , Bolsa de Fabricius/virologia , Galinhas , Metilação de DNA/imunologia , Resistência à Doença/imunologia , Epigênese Genética , Genoma/genética , Virus da Influenza A Subtipo H5N1/imunologia , Influenza Aviária/imunologia , Influenza Aviária/virologia , Transdução de Sinais , Baço/imunologia , Baço/virologia , Timo/imunologia , Timo/virologia
19.
Genet Mol Res ; 13(4): 10501-9, 2014 Dec 12.
Artigo em Inglês | MEDLINE | ID: mdl-25511033

RESUMO

The objective of this study was to examine the clinical findings, magnetic resonance imaging (MRI), pathological features, and treatment experiments of patients with hypertrophic cranial pachymeningitis (HCP). The clinical findings, MRI, and pathological appearances of 9 patients with HCP were analyzed retrospectively. The thickened dura mater was markedly enhanced after contrast media injection. The lesion near the brain hemisphere presented long regions of T1- and T2-weighted abnormal signal intensities. The abnormal signal intensities of the brain tissue were decreased significantly. Pathological examination demonstrated chronic inflammation changes, with cerebral dura mater fibrous tissue showing obvious hyperplasia, and the periphery of the blood vessel showing a great quantity of infiltrating phlegmonosis cells. HCP mainly presents headache and paralysis of multiple cranial nerves. The distinctive signs on brain MRIs involve strengthening the signal in the cerebral dura.


Assuntos
Dura-Máter/diagnóstico por imagem , Imageamento por Ressonância Magnética , Meningite/diagnóstico por imagem , Adulto , Angiografia , Nervos Cranianos/diagnóstico por imagem , Nervos Cranianos/fisiopatologia , Dura-Máter/fisiopatologia , Feminino , Cefaleia/fisiopatologia , Humanos , Masculino , Meningite/fisiopatologia , Pessoa de Meia-Idade
20.
Asian-Australas J Anim Sci ; 27(5): 706-16, 2014 May.
Artigo em Inglês | MEDLINE | ID: mdl-25050006

RESUMO

A new strategy of co-inoculating Bacillus subtilis MA139 with Streptococcus thermophilus and Saccharomyces cerevisiae was used to produce fermented soybean meal (FSBM). Three experiments were conducted to determine the concentration of digestible energy (DE) and metabolizable energy (ME) (Exp. 1), apparent ileal digestibility (AID) and standardized ileal digestibility (SID) of amino acids (AA) (Exp. 2), and feeding value (Exp. 3) of FSBM produced by this new strategy (NFSB) compared with soybean meal (SBM) and conventionally available FSBM (Suprotein). In Exp. 1, twenty-four barrows (initial body weight [BW] of 32.2 ±1.7 kg) were randomly allotted to 1 of 4 diets with 6 replicates per diet. A corn basal diet and 3 diets based on a mixture of corn and 1 of 3 soybean products listed above were formulated and the DE and ME contents were determined by the difference method. The results showed that there were no differences in DE and ME between SBM and either FSBM product (p>0.05). In Exp. 2, eight barrows (initial BW of 26.8±1.5 kg) were fitted with ileal T-cannulaes and used in a replicated 4×4 Latin square design. Three corn-starch-based diets were formulated using each of the 3 soybean products as the sole source of AA. A nitrogen-free diet was also formulated to measure endogenous losses of AA. The results showed that the SID of all AA except arginine and histidine was similar for NFSB and SBM (p>0.05), but Suprotein had greater (p<0.05) SID of most AA except lysine, aspartate, glycine and proline than NFSB. In Exp. 3, a total of 144 piglets (initial BW of 8.8±1.2 kg) were blocked by weight and fed 1 of 4 diets including a control diet with 24% SBM as well as diets containing 6% and 12% NFSB or 12% Suprotein added at the expense of SBM. During d 15 to 28, replacing SBM with 6% NFSB significantly improved average daily gain (ADG) and average daily feed intake (ADFI) (p<0.05) for nursery piglets. During the overall experiment, ADG of piglets fed diets containing 6% NFSB was significantly greater (p<0.05) than that of piglets fed SBM. In conclusion, fermentation with the new strategy did not affect the energy content or the AID and the SID of AA in SBM. However, inclusion of 6% NFSB in diets fed to nursery piglets improved performance after weaning likely as a result of better nutritional status and reduced immunological challenge.

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