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1.
Sci Rep ; 10(1): 10000, 2020 Jun 19.
Artigo em Inglês | MEDLINE | ID: mdl-32561767

RESUMO

OBJECTIVES: In this in vivo animal study, we evaluated the effect of plasma electrolytic oxidation (PEO) coating on the topographic and biological parameters of implants installed in rats with induced osteoporosis and low-quality bones. MATERIALS AND METHODS: In total 44 Wistar rats (Rattus novergicus), 6 months old, were submitted to ovariectomy (OXV group) and dummy surgery (SHAM group). After 90 days, the ELISA test was performed and the ovariectomy effectiveness was confirmed. In each tibial metaphysis, an implant with PEO coating containing Ca2+ and P5+ molecules were installed, and the other tibia received an implant with SLA acid etching and blasting (AC) (control surface). After 42 days, 16 rats from each group were euthanized, their tibias were removed for histological and immunohistochemical analysis (OPG, RANKL, OC and TRAP), as well as reverse torque biomechanics. Data were submitted to One-way ANOVA or Kruskal-Wallis tests, followed by a Tukey post-test; P < 0.05. Histological analyses showed higher bone neoformation values among the members of the PEO group, SHAM and OVX groups. Immunohistochemical analysis demonstrated equilibrium in all groups when comparing surfaces for TRAP, OC and RANKL (P > 0.05), whereas OPG showed higher PEO labeling in the OVX group (P < 0.05). Biomechanical analysis showed higher reverse torque values (N.cm) for PEO, irrespective of whether they were OVX or SHAM groups (P < 0.05). CONCLUSION: The results indicated that the PEO texturing method favored bone formation and showed higher bone maturation levels during later periods in osteoporotic rats.

2.
Materials (Basel) ; 13(7)2020 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-32244631

RESUMO

Plasma electrolytic oxidation (PEO) has been a promising surface coating with better mechanical and antimicrobial parameters comparing to conventional treatment surfaces. This study evaluated the peri-implant bone repair using (PEO) surface coatings compared with sandblasted acid (SLA) treatment. For this purpose, 44 Wistar rats were ovariectomized (OVX-22 animals) or underwent simulated surgery (SS-22 animals) and received implants in the tibia with each of the surface coatings. The peri-implant bone subsequently underwent molecular, microstructural, bone turnover, and histometric analysis. Real-time PCR showed a higher expression of osteoprotegerin (OPG), receptor activator of nuclear kappa-B ligand (RANKL), and osteocalcin (OC) proteins in the SLA/OVX and PEO/SS groups (p < 0.05). Computed microtomography, confocal microscopy, and histometry showed similarity between the PEO and SLA surfaces, with a trend toward the superiority of PEO in OVX animals. Thus, PEO surfaces were shown to be promising for enhancing peri-implant bone repair in ovariectomized rats.

3.
Clin Oral Investig ; 24(5): 1637-1650, 2020 May.
Artigo em Inglês | MEDLINE | ID: mdl-31338633

RESUMO

OBJECTIVES: The effect of leucocyte- and platelet-rich fibrin (L-PRF), associated with DBBM (deproteinized bovine bone mineral; Bio-Oss®) was investigated and compared with autogenous bone graft as a standard material for filling bone defects. MATERIAL AND METHODS: A defect of 5 mm in diameter was performed in 40 calvaria of rats. The animals were divided into 5 groups and received blood clot (CO), autogenous bone (AUT), DBBM (BIO), L-PRF, or DBBM associated with L-PRF (BIO-LPRF). After 4 and 8 weeks, bone regeneration was assessed by histometric and immunohistochemical analyses. RESULTS: The highest mean percentage of bone formation found at 4 and 8 weeks was observed for the BIO-L-PRF group (54.0% ± 2.8 and 63.6% ± 2.2). The lowest mean percentage at 4 and 8 weeks was observed for the CO group (16.7% ± 2.5 and 20.5% ± 1.0). There was statistical similarity among the AUT, BIO, and L-PRF groups. The expressions OC, RUNX 2, and VEGF showed a favorable aspect in the formation of new bone for BIO-L-PRF. VEGF was the marker with the highest expression because it was related to the initial healing process, promoting the migration and proliferation of endothelial cells in the region of the defect. Even after weeks, VEGF maintained a moderate expression. CONCLUSIONS: The association of L-PRF with DBBM improved bone repair when these biomaterials were inserted into the defects of the calvaria of rats. CLINICAL RELEVANCE: This reinforces the good performance of bovine bone and L-PRF as filler materials, especially when associated.

4.
Clin Implant Dent Relat Res ; 21(6): 1175-1180, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31691471

RESUMO

OBJECTIVES: The objective of this study was to evaluate the peri-implant bone tissue formation around titanium implants with different surface treatments, placed in bone defects filled or not with bone substitute material (BSM). MATERIALS AND METHODS: Ten animals were divided into two groups according to implant surface treatment. In each tibia, a bone defect was created followed by the placement of one implant. On the left tibia, the defect was filled with blood clot (BC), and on the right tibia, the defect was filled with biphasic hydroxyapatite/ß-tricalcium-phosphate (HA/TCP) generating four subgroups: BC-N: blood clot and porous surface; BC-A: blood clot and porous-hydrophilic surface; HA/TCP-N: BSM and porous surface; HA/TCP-A: BSM and porous-hydrophilic surface. The animals were submitted to euthanasia 60 days after implant installation. After light-curing resin inclusion, the blocks containing the implant and the bone tissue were stained and evaluated by means of histomorphometry to assess the percentages of bone implant contact (% BIC). Data was normally distributed and the group differences were examined using the parametric tests of Two-Way ANOVA. RESULTS: The BC-A group presented the higher mean value of BIC (46.43%). The HA/TCP-A group presented the higher mean value of BIC. The porous-hydrophilic surfaces presented better results of BIC when compared to the porous surface in both conditions of defect filling. No statistically significant differences were found among all groups (95% confidence interval and P < .05). CONCLUSION: According to histomorphometric analysis, after 60-days in a rabbit model, hydrophilic and hydrophobic surfaces have the same behavior in the presence or absence of HA/TCP.


Assuntos
Substitutos Ósseos , Implantes Dentários , Trombose , Animais , Durapatita , Osseointegração , Projetos Piloto , Coelhos , Propriedades de Superfície , Titânio
5.
J Appl Oral Sci ; 27: e20180574, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31596365

RESUMO

OBJECTIVES: Hypertension is one of the main causes of premature death in the world; also, it is associated with several bone alterations. Preclinical studies have demonstrated delayed alveolar bone healing in hypertensive rats. However, losartan has been favorable for consolidation of bone grafts and reduction in active periodontitis. Therefore, losartan is suggested to be effective in bone formation stages, as well as in the synthesis of matrix proteins and mineralization. To evaluate the alveolar bone dynamics in hypertensive rats treated with losartan by laser confocal microscopy and histological analysis. METHODOLOGY: Thirty-two rats, 16 spontaneously hypertensive rats (SHR) and 16 Wistar albinus rats, treated or not with losartan (30 mg/kg/day) were used. Calcein fluorochrome at 21 days and alizarin red fluorochrome at 49 days were injected in rats (both 20 mg/kg). The animals were submitted to euthanasia 67 days after treatment, and then the right maxilla was removed for laser confocal microscopy analysis and the left maxilla for histological analysis. RESULTS: This study showed a greater calcium marking in normotensive animals treated with losartan in relation to the other groups. Laser confocal microscopy parameters showed higher values of bone volume formed, mineralized surface, active surface of mineralization and bone formation rate in normotensive animals treated with losartan. However, a smaller mineralized surface was observed in all hypertensive animals. CONCLUSION: Losartan can improve bone mineralization parameters under normal physiological conditions, but the same anabolic effect does not occur under hypertension.


Assuntos
Processo Alveolar/efeitos dos fármacos , Processo Alveolar/fisiopatologia , Anti-Hipertensivos/farmacologia , Hipertensão/fisiopatologia , Losartan/farmacologia , Processo Alveolar/patologia , Animais , Pressão Sanguínea/efeitos dos fármacos , Regeneração Óssea/efeitos dos fármacos , Calcificação Fisiológica/efeitos dos fármacos , Fluoresceínas/análise , Masculino , Microscopia Confocal , Osteogênese/efeitos dos fármacos , Ratos Endogâmicos SHR , Ratos Wistar , Reprodutibilidade dos Testes , Fatores de Tempo
6.
J Craniofac Surg ; 30(7): e629-e630, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31157639

RESUMO

Gunshot wounds can cause extensive destruction of soft tissue and bone, and the maxillofacial region is often affected. The reconstructive treatment is complex due to the difficulty to reestablish the central arch mandibular contour and volume. Moreover, the goal is to avoid postoperative infections and obtain bone graft neovascularization. Therefore, this report shows a clinical case of a patient presenting mandibular deformity with a mandibular central arch discontinuity caused by a firearm injury. The strategy to the reconstructive treatment as performed with a condensed and stabilized particulate autogenous bone graft was essential and effectiveness. After 1 year, it was obtained a satisfactory result to the patient.


Assuntos
Mandíbula/cirurgia , Reconstrução Mandibular , Ferimentos por Arma de Fogo/cirurgia , Adulto , Transplante Ósseo , Humanos , Masculino , Procedimentos Cirúrgicos Reconstrutivos , Transplante Autólogo
7.
Ultrason Sonochem ; 56: 437-446, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31101282

RESUMO

The aim of this study was determinate the best sonochemical time in order to obtain better bone characteristics when a bioactive material (Biogran) is used in the filling periimplantar defects. In this study, 32 rats were submitted to surgical proceedings to create a periimplantar defect that was filled with Biogran receiving different sonochemical times: 15 (G1), 30 (G2), 45 (G3) or 90 min (G4). The biomaterial was characterized through X-ray diffraction and scanning electron microscopy (SEM). In vivo analysis was performed through micro CT, laser confocal microscopy, immunohistochemistry and evaluation of bone cytoarchitecture through hematoxylin and eosin (HE) staining. The data were submitted to statistical testing, considering a significance level of p < 0.05. Rx diffraction of pure bioglass showed that it is predominantly amorphous; otherwise, there are small peaks at 23° and 31°. SEM shows that the longer the sonochemical time, the less edges the biomaterial will present. Within the groups, G1 and G2 showed the best quantity and quality by micro CT (p > 0.05). The best bone turnover result was found in G1 and G2, otherwise the better results were related to neoformed bone area, bone mineral apposition rate and bone implant contact to G1 (p < 0.05). G1 had the best results in terms of bone cytoarchitectural evaluation and immunohistochemistry. It is possible to conclude that Biogran that received 15 min of sonochemical treatment (G1) presented periimplantar bone repair with the best extracellular matrix properties, including the best quality and quantity of vital bone.


Assuntos
Prótese Ancorada no Osso , Cerâmica/química , Ondas Ultrassônicas , Animais , Masculino , Ratos , Padrões de Referência , Tíbia/cirurgia
8.
Arch Oral Biol ; 102: 147-154, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-31022626

RESUMO

OBJECTIVE: The aim of this study was to evaluate bone metabolism in the alveolar repair process in orchiectomized male rats treated with teriparatide. MATERIALS AND METHODS: A total of 78 rats were divided into three groups: 26 oriectomized rats treated with teriparatide (ORQTRAT), 26 untreated orchiectomized rats (ORQ), and 26 rats which had undergone sham surgical procedures (SHAM), all these animals underwent extraction of the central incisor tooth. Thus, a histological analysis was performed (42 days). Real Time PCR (14 and 42 days) and immunohistochemical (42 days) analyses were performed based on the expression of RANK-L and osteoprotegerin (OPG). The calcein and alizarin were analyzed via laser confocal microscopy to verify alveolar bone turnover, and microtomographic analysis was performed to determine volume and bone quality (calcified tissues). In this analysis, the euthanasia period was 60 days post-extraction. The quantitative data were submitted to the statistical test and the significance level of 5% was adopted. RESULTS: Teriparatide increased bone turnover with a higher relative gene expression of RANKL and OPG at 14 days, and at 42 days, there was a significant decrease of RANKL and an increase of OPG, this standard can also be evaluated in the ratio of RANKL to OPG. Greater values of area and bone mineral deposition were found for the ORQTRAT group (p < 0.05), along with higher bone volume values (p < 0.05). The immunolabels revealed greater intensity in the relationship of RANKL and OPG, which led to intense remodeling aiding in the process of bone formation. CONCLUSION: The teriparatide treatment in orchiectomized rats increases bone volume and decreases the porosity, in addition to promoting greater intensity in bone turnover during alveolar repair.


Assuntos
Extração Dentária , Processo Alveolar , Animais , Remodelação Óssea , Incisivo , Masculino , Osteoprotegerina , Ligante RANK , Ratos , Teriparatida
9.
J Craniofac Surg ; 30(4): 1016-1021, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-30908445

RESUMO

This study aimed to assess the repair of surgically created bone defects filled with blood clot, autogenous bone, and calcium phosphate cement, by histomorphometric and immunohistochemical analyses. Ten adult male rabbits were used. Three bone defects were prepared with an 8-mm diameter trephine bur in the parietal region of each animal and filled with blood clot (Group BC), autogenous bone (Group AB), and calcium phosphate bone cement (Group CPC). The animals were euthanized at 40 and 90 postoperative days. The sections were subjected to histomorphometric analysis of the new bone formed inside the calvarial defects and immunohistochemical staining to determine the expression of osteocalcin (OC), osteopontin (OP), and tartrate-resistant acid phosphatase (TRAP) proteins. Histomorphometric data were analyzed statistically by analysis of variance and Tukey's post hoc test at 5% significance level. In the results at 40 and 90 days, Group AB differed significantly from Group CPC regarding the area of newly formed bone. The immunohistochemical analysis revealed expression of OP, OC, and TRAP proteins in all groups. Group AB showed prevalence of OC and OP, and lower TRAP expression. Therefore, the calcium phosphate bone cement assessed in the present study did not accelerate the protein expression dynamics during bone healing, compared with the autogenous group.


Assuntos
Cimentos para Ossos , Regeneração Óssea/efeitos dos fármacos , Fosfatos de Cálcio/farmacologia , Crânio/patologia , Animais , Materiais Biocompatíveis , Substitutos Ósseos/farmacologia , Transplante Ósseo , Modelos Animais de Doenças , Imuno-Histoquímica , Masculino , Coelhos , Distribuição Aleatória , Crânio/efeitos dos fármacos , Trombose , Cicatrização/efeitos dos fármacos
11.
Clin Oral Investig ; 23(5): 2313-2322, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-30291494

RESUMO

OBJECTIVE: To evaluate intermittent teriparatide therapy's influence on the alveolar healing process in rats with induced osteoporosis. MATERIALS AND METHODS: Fifty-four male rats were divided into three groups: fictitious surgery (SHAM), bilateral orchiectomy (ORQ NT), and bilateral orchiectomy treated with teriparatide (ORQ TERI). Right upper incisor extraction was performed. After 14 and 42 days, the rats were euthanized. Immunolabeling analysis was performed in order to characterize bone turnover through Wnt, alcaline phosphatase, osteocalcin, and TRAP presence in tissue; micro-CT analysis was performed in order to determine the bone volume fraction (BV/TV), trabecular thickness, separation, and number (Tb.Th, Tb.Sp, Tb.N). For the micro-CT data, statistical analysis was performed through one-way ANOVA and Tukey post-test, for parametrical data, with significance level adopted in p < 0.05. Days, Wnt, alkaline phosphatase, and osteocalcin revealed more labeling for ORQ TERI and less for TRAP. For the Tb.N in the ORQ NT group was 0.496 mm, a significant statistical difference was observed between the groups of ORQ NT and ORQ TERI (p < 0.05). For the BV/TV, Tb.Sp, and Tb.Th parameters, no significant statistical difference was observed among the three experimental groups (p > 0.05). CONCLUSIONS: Treatment with intermittent teriparatide reverted impairment in the metabolism of repairing bone tissue in orchiectomized animals. CLINICAL RELEVANCE: Cases of decreased bone density such as osteoporosis can lead to delayed alveolar repair process. PTH 1-34 has been shown to be a medication that improves this repair, making bone of low quality into one with normal features.


Assuntos
Remodelação Óssea , Osteoporose/tratamento farmacológico , Teriparatida/farmacologia , Animais , Densidade Óssea , Masculino , Orquiectomia , Osteoporose/induzido quimicamente , Ratos , Ratos Sprague-Dawley
12.
Bone ; 120: 101-113, 2019 03.
Artigo em Inglês | MEDLINE | ID: mdl-30339908

RESUMO

This study evaluated the effects of antimicrobial photodynamic therapy (aPDT) in the alveolar repair of rats with major risk factors for bisphosphonate-related osteonecrosis of the jaws (BRONJ). Senile rats received 0.45 ml of vehicle (VEH and VEH/aPDT) or 0.45 ml of zoledronate (ZOL and ZOL/aPDT) every three days for seven weeks. After three weeks of treatment, the first lower left molar was extracted. VEH/aPDT and ZOL/aPDT were submitted to aPDT on the extraction site at 0, 2 and 4 days postoperatively. Euthanasia was performed 28 days postoperatively and the extraction site was evaluated by clinical, histological, histometric, histochemical and immunohistochemical analysis. ZOL showed tissue repair impairment; lower percentage of newly formed bone tissue (NFBT); higher percentage of non-vital bone tissue (NVBT); fewer mature collagen fibers and increased immunolabeling for tumor necrosis factor (TNFα), interleukin (IL)-1ß and IL-6. ZOL/aPDT showed clinical and histological characteristics of the extraction site, percentage of NFBT and percentage of mature collagen fiber similar to VEH. Percentage of NVBT and immunolabeling for inflammatory cytokines in ZOL/aPDT was lower than in ZOL. Immunolabeling for tartarato-resistant acid phosphatase (TRAP) was lower in ZOL and ZOL/aPDT. aPDT in the dental extraction site improves tissue repair process and prevents the occurrence of BRONJ-like lesions after tooth extraction.


Assuntos
Processo Alveolar/patologia , Anti-Infecciosos/uso terapêutico , Osteonecrose da Arcada Osseodentária Associada a Difosfonatos/tratamento farmacológico , Osteonecrose da Arcada Osseodentária Associada a Difosfonatos/etiologia , Fotoquimioterapia , Extração Dentária/efeitos adversos , Ácido Zoledrônico/efeitos adversos , Envelhecimento/patologia , Processo Alveolar/efeitos dos fármacos , Animais , Osteonecrose da Arcada Osseodentária Associada a Difosfonatos/prevenção & controle , Colágeno/metabolismo , Feminino , Interleucina-1beta/metabolismo , Interleucina-6/metabolismo , Mucosa Bucal/patologia , Osteogênese , Ratos Wistar , Fosfatase Ácida Resistente a Tartarato/metabolismo , Fator de Necrose Tumoral alfa/metabolismo , Cicatrização/efeitos dos fármacos
13.
J. appl. oral sci ; 27: e20180574, 2019. graf
Artigo em Inglês | LILACS-Express | LILACS | ID: biblio-1040233

RESUMO

Abstract Hypertension is one of the main causes of premature death in the world; also, it is associated with several bone alterations. Preclinical studies have demonstrated delayed alveolar bone healing in hypertensive rats. However, losartan has been favorable for consolidation of bone grafts and reduction in active periodontitis. Therefore, losartan is suggested to be effective in bone formation stages, as well as in the synthesis of matrix proteins and mineralization. Objectives: To evaluate the alveolar bone dynamics in hypertensive rats treated with losartan by laser confocal microscopy and histological analysis. Methodology: Thirty-two rats, 16 spontaneously hypertensive rats (SHR) and 16 Wistar albinus rats, treated or not with losartan (30 mg/kg/day) were used. Calcein fluorochrome at 21 days and alizarin red fluorochrome at 49 days were injected in rats (both 20 mg/kg). The animals were submitted to euthanasia 67 days after treatment, and then the right maxilla was removed for laser confocal microscopy analysis and the left maxilla for histological analysis. Results: This study showed a greater calcium marking in normotensive animals treated with losartan in relation to the other groups. Laser confocal microscopy parameters showed higher values of bone volume formed, mineralized surface, active surface of mineralization and bone formation rate in normotensive animals treated with losartan. However, a smaller mineralized surface was observed in all hypertensive animals. Conclusion: Losartan can improve bone mineralization parameters under normal physiological conditions, but the same anabolic effect does not occur under hypertension.

14.
Implant Dent ; 27(6): 615-622, 2018 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-30188365

RESUMO

PURPOSE: The study aimed to investigate through histology, histometry, and immunohistochemistry the vital bone formation after grafting of biphasic calcium phosphate bioceramic (BC) in combination with calvarial autogenous bone into the dental sockets of rats. MATERIALS AND METHODS: Forty-five male rats were submitted to upper right incisor extraction and divided according to the grafted material in: control, bioceramic (BC), and bioceramic + autogenous bone (BC + AB). The animals were killed 7, 21, and 42 days after surgery for histological, histometric, and immunohistochemistry analysis. RESULTS: Histomorphometric results demonstrated, for BC + AB group, formation of trabecular bone between the particles of BCs and autogenous bone, connecting them, as well as higher percentage of vital bone in comparison with BC. Immunohistochemical reactions showed intense labeling for Runx2-positive cells in the group BC + AB. CONCLUSIONS: Autogenous bone was able to stimulate bone turnover enabling a larger amount of vital bone synthesis and can be recommended as a viable grafting material in combination with synthetic biphasic BC.


Assuntos
Materiais Biocompatíveis , Transplante Ósseo , Cerâmica , Hidroxiapatitas , Osteogênese , Alvéolo Dental/patologia , Animais , Masculino , Ratos , Ratos Wistar , Crânio/transplante , Alvéolo Dental/cirurgia
15.
Artigo em Inglês | MEDLINE | ID: mdl-30264913

RESUMO

OBJECTIVE: Hypertension is not only associated with cardiovascular diseases but also with alterations in bone quality. Hypertension therefore might be a risk factor for osseointegration. Preclinical studies suggest that losartan, an angiotensin II receptor blocker widely used to treat hypertension, has a beneficial effect in graft consolidation. Here, we determine the effect of hypertension and losartan on osseointegration. METHODS: We used spontaneously hypertensive rats (SHR) and normotensive Wistar albinus rats receiving losartan (30 mg/kg, p.o.) or left untreated. After 1 week, titanium miniscrews were inserted into the tibia. Sixty days after implantation, implant stability was evaluated by removal torque measurement considered the primary endpoint. Microcomputed tomography and histomorphometric analysis were secondary endpoints. RESULTS: Losartan increased the removal torque in the hypertensive SHR group to levels of the Wistar controls. While the cortical parameters of osseointegration remained unchanged, losartan increased medullary bone formation. Microcomputed tomography revealed a higher bone volume per tissue volume and trabecular thickness in the SHR rats treated with losartan. Histomorphometric analysis further showed that losartan significantly increased the thickness of newly formed bone in medullary area in hypertensive SHR rats. Losartan did not significantly alter the parameters of osseointegration in normotensive rats. CONCLUSIONS: The data presented suggest that the angiotensin II receptor antagonist losartan increases the medullary parameters of osseointegration in a tibia model of spontaneously hypertensive rats. Considering the study limitations, understanding the impact of hypertension and the respective drugs on osseointegration requires further research.

16.
Arch. Health Invest ; 7(9): 388-391, set. 2018. ilus
Artigo em Português | BBO - Odontologia | ID: biblio-994466

RESUMO

O tumor de Warthin é a segunda lesão mais comum da glândula parótida, sendo raro em qualquer outra glândula salivar. Possui comportamento benigno, com crescimento lento e indolor. Neste artigo é relatado um caso clínico onde o tumor apresentou evolução de um ano, sem sintomatologia associada, gerando assimetria facial ao paciente, sendo realizado tratamento cirúrgico conservador apenas por enucleação da lesão, visando reduzir cirurgias maiores como a parotidectomias parciais ou totais. Após acompanhamento de 5 anos o paciente não apresenta sequelas do tratamento cirúrgico ou sinal de recorrência da lesão(AU)


Warthin's tumor is the second most common lesion of the parotid gland, being rare in any other salivary gland. It has benign behavior, with slow and painless growth. In this article a clinical case was reported where the tumor presented evolution of one year, with no associated symptomatology, generating facial asymmetry to the patient, being carried out conservative surgical treatment only by enucleating of the lesion, aiming to reduce major surgeries such as partial or total parotidectomy. After 5 years of follow-up, the patient did not present sequel of the surgical treatment or a sign of recurrence of the lesion(AU)


El tumor de Warthin es la segunda lesión más común de la glándula parótida, siendo raro en cualquier otra glándula salivar. El mismo tiene comportamiento benigno, con un crecimiento lento e indoloro. En este artículo se relata un caso clínico donde el tumor presentó evolución de un año, sin sintomatología asociada, generando asimetría facial al paciente, siendo realizado tratamiento quirúrgico conservador sólo por enucleación de la lesión, buscando reducir las cirugías mayores como la parotidectomías parciales o totales. Después del seguimiento de 5 años el paciente no presenta secuelas del tratamiento quirúrgico ni señal de recurrencia de la lesión(AU)


Assuntos
Humanos , Feminino , Pessoa de Meia-Idade , Cirurgia Bucal , Adenolinfoma/cirurgia , Glândula Parótida/cirurgia , Adenolinfoma
17.
J Appl Oral Sci ; 26: e20170470, 2018 Jul 10.
Artigo em Inglês | MEDLINE | ID: mdl-29995145

RESUMO

INTRODUCTION: The hypothesis of this study was that the peri-implant bone healing of the group of pinealectomized rats would differ from the control group. The samples were subjected to immunohistochemical, microtomographic (total porosity and connectivity density), and fluorochrome (mineralized surface) analyses. OBJECTIVES: The goal of this study was to investigate the cellular changes and bone remodeling dynamics along the bone/implant interface in pinealectomized rats. MATERIAL AND METHODS: The total of 18 adult male rats (Rattus norvegicus albinus, Wistar) was divided into three groups (n=6): control (CO), pinealectomized without melatonin (PNX) and pinealectomized with melatonin (PNXm). All animals were submitted to the first surgery (pinealectomy), except the CO group. Thirty days after the pinealectomy without melatonin, the second surgery was conducted, in which all animals received an implant in each tibia (36 titanium implants with surface treatment were installed - Implalife® São Paulo, SP, Brazil). By gavage, the rats of the PNX group received the vehicle solution, and the procedure. RESULTS: Immunohistochemical analysis for runt-related transcription factor 2 (RUNX2), alkaline phosphatase (ALP), osteopontin (OP) and osteocalcin (OC) showed that the bone repair process in the PNXm group was similar to that of the CO group, whereas the PNX group showed a delay. The microtomographic parameters of total porosity [Po(tot)] and bone surface (BS) showed no statistically significant differences, whereas for the connective density (Conn.Dn) a statistical difference was found between the CO and PNXm groups. Fluorochrome analysis of the active mineralized surface showed statistically significant difference between the CO and PNX and between the CO and PNXm groups. CONCLUSION: The absence of the pineal gland impaired the bone repair process during osseointegration, however the daily melatonin replacement was able to restore this response.


Assuntos
Conservadores da Densidade Óssea/farmacologia , Interface Osso-Implante , Melatonina/farmacologia , Osseointegração/efeitos dos fármacos , Glândula Pineal/cirurgia , Fosfatase Alcalina/análise , Animais , Subunidade alfa 1 de Fator de Ligação ao Core/análise , Implantação Dentária Endo-Óssea , Corantes Fluorescentes , Imuno-Histoquímica , Implantes Experimentais , Masculino , Osteocalcina/análise , Osteopontina/análise , Ratos Wistar , Reprodutibilidade dos Testes , Tíbia/efeitos dos fármacos , Tíbia/patologia , Tíbia/cirurgia , Titânio , Resultado do Tratamento , Microtomografia por Raio-X
18.
J Craniofac Surg ; 29(6): e624-e625, 2018 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-30052614

RESUMO

Orbital emphysema can be a rare and severe postoperative complication. Fast and critical diagnosis is essential to avoid permanent visual loss to the patient. Therefore, a case of a male patient victim of a motorcycle accident with a postoperative complication of orbital emphysema has been presented. The immediate diagnosis and treatment of the orbital emphysema revealed to be the main success factors for the resolution of this complication.


Assuntos
Enfisema/etiologia , Doenças Orbitárias/etiologia , Complicações Pós-Operatórias/etiologia , Adulto , Humanos , Masculino , Fraturas Orbitárias/cirurgia
19.
J Appl Oral Sci ; 26: e20170296, 2018 Jun 11.
Artigo em Inglês | MEDLINE | ID: mdl-29898173

RESUMO

OBJECTIVE: The aim of this study was to compare the bone resorption rate, histomorphometry and immunohistochemical findings of bioactive glass (Biogran; Biomet, Warsaw, IN, USA) mixed with autogenous bone grafts (1:1) and autogenous bone graft isolate in maxillary sinus elevation surgery. MATERIAL AND METHODS: A total of 9 maxillary sinuses were grafted with Biogran with autogenous bone graft (group 1) and 12 were mixed with autogenous bone graft (group 2). Postoperative cone beam computed tomography (CBCT) was used to measure the initial graft volume after 15 days (T1), and 6 months later, another CBCT scan was performed to evaluate the final graft volume (T2) and determine the graft resorption rate. The resorption outcomes were 37.9%±18.9% in group 1 and 45.7%±18.5% in group 2 (P=0.82). After 6 months, biopsies were obtained concurrent with the placement of dental implants; these implants were subjected to histomorphometric analysis and immunohistochemical analysis for tartrate-resistant acid phosphatase (TRAP). RESULTS: The average bone formation in group 1 was 36.6%±12.9 in the pristine bone region, 33.2%±13.3 in the intermediate region, and 45.8%±13.8 in the apical region; in group 2, the values were 34.4%±14.4, 35.0%±13.9, and 42.0%±16.6 of new bone formation in the pristine bone, intermediate, and apical regions, respectively. Immunostaining for TRAP showed poor clastic activity in both groups, which can indicate that those were in the remodeling phase. CONCLUSIONS: The similarity between the groups in the formation and maintenance of the graft volume after 6 months suggests that the bioactive glass mixed with autogenous bone (1:1) can be used safely as a bone substitute for the maxillary sinus lift.


Assuntos
Perda do Osso Alveolar/patologia , Substitutos Ósseos/química , Substitutos Ósseos/uso terapêutico , Transplante Ósseo , Vidro/química , Seio Maxilar/cirurgia , Levantamento do Assoalho do Seio Maxilar/métodos , Regeneração Óssea/fisiologia , Tomografia Computadorizada de Feixe Cônico , Humanos , Imuno-Histoquímica , Seio Maxilar/patologia , Osteogênese/fisiologia , Estudos Prospectivos , Reprodutibilidade dos Testes , Estatísticas não Paramétricas , Fatores de Tempo , Transplante Autólogo/métodos , Resultado do Tratamento
20.
J Appl Oral Sci ; 26: e20170326, 2018 06 18.
Artigo em Inglês | MEDLINE | ID: mdl-29898174

RESUMO

INTRODUCTION: Alveolar bone healing after upper incisor extraction in rats is a classical model of preclinical studies. The underlying morphometric, cellular and molecular mechanism, however, remains imprecise in a unique study. OBJECTIVES: The aim of this study was therefore to characterize the alveolar bone healing after upper incisor extraction in rats by micro computed tomographic (Micro-CT), immunohistochemical and real-time polymerase chain reaction (RT-PCR) analysis. MATERIAL AND METHODS: Thirty animals (Rattus norvegicus, Albinus Wistar) were divided into three groups after upper incisors extraction at 7, 14, and 28 days. Micro-CT was evaluated based on the morphometric parameters. Subsequently, the histological analyses and immunostaining of osteoprotegerin (OPG), receptor activator of nuclear kappa B ligand (RANKL) and tartrate resistant acid phosphate (TRAP) was performed. In addition, RT-PCR analyses of OPG, RANKL, the runt-related transcription factor 2 (RUNX2), osteocalcin (OC), osteopontin (OPN), osterix (OST) and receptor activator of nuclear kappa B (RANK) were performed to determine the expression of these proteins in the alveolar bone healing. RESULTS: Micro-CT: The morphometric parameters of bone volume and trabecular thickness progressively increased over time. Consequently, a gradual decrease in trabecular separation, trabecular space and total bone porosity was observed. Immunohistochemical: There were no differences statistically significant between the positive labeling for OPG, RANKL and TRAP in the different periods. RT-PCR: At 28 days, there was a significant increase in OPG expression, while RANKL expression and the RANKL/OPG ratio both decreased over time. CONCLUSION: Micro-CT showed the newly formed bone had favorable morphometric characteristics of quality and quantity. Beyond the RUNX2, OC, OPN, OST, and RANK proteins expressed in the alveolar bone healing, OPG and RANKL activity showed to be essential for activation of basic multicellular units during the alveolar bone healing.


Assuntos
Remodelação Óssea/fisiologia , Alvéolo Dental/diagnóstico por imagem , Alvéolo Dental/fisiologia , Cicatrização/fisiologia , Animais , Subunidade alfa 1 de Fator de Ligação ao Core/análise , Expressão Gênica , Imuno-Histoquímica , Masculino , Osteocalcina/análise , Osteopontina/análise , Osteoprotegerina/análise , Ligante RANK/análise , Ratos Wistar , Valores de Referência , Reprodutibilidade dos Testes , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Fosfatase Ácida Resistente a Tartarato/análise , Fatores de Tempo , Extração Dentária , Fatores de Transcrição/análise , Microtomografia por Raio-X
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