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1.
Dent Mater J ; 2021 Jan 30.
Artigo em Inglês | MEDLINE | ID: mdl-33518688

RESUMO

This study aimed to evaluate the mechanical properties of contemporary heat-treated nickel-titanium (NiTi) rotary instruments used in the single-length technique [ProTaper Next (PTN), HyFlex EDM (EDM), and JIZAI (JZ)]. Bending loads, cyclic fatigue resistance, torque/force values and canal-centering ratios were evaluated for the three instruments and a non-heat-treated experimental NiTi instrument with the same geometry as JZ (nJZ). EDM and JZ exhibited significantly lower bending load and more cycles to failure compared with nJZ and PTN (p<0.05). PTN and JZ exhibited significantly better centering ability than nJZ and EDM (p<0.05). JZ and nJZ generated significantly smaller upward force and maximum torque than PTN and EDM (p<0.05). Under the present experimental condition, JZ exhibited flexibility and cyclic fatigue resistance comparable to EDM, better maintained the canal curvature than the other instruments, and generated smaller torque and screw-in force than PTN and EDM.

2.
J Oral Sci ; 2020 Dec 10.
Artigo em Inglês | MEDLINE | ID: mdl-33298628

RESUMO

The purpose of this study was to examine the effect of heating on the physical properties of four types of premixed calcium silicate-based root canal sealers. Endoseal MTA, Well-Root ST, EndoSequence BC Sealer, EndoSequence BC Sealer HiFlow, and AH Plus (epoxy resin root canal sealer) were heated at 100°C for 1 min, and changes in setting time, flow, and film thickness were evaluated in accordance with ISO 6876:2012 standards. In addition, pH measurement and scanning electron microscopy (SEM) and energy-dispersive X-ray spectroscopy (EDS) analyses of the set materials were performed. All root canal sealers heated at 100°C showed significant decreases in setting time and flow, particularly Endoseal MTA. In addition, the film thickness of Endoseal MTA increased significantly after heating at 100°C. The pH and SEM/EDS results were not affected by heating. Heating calcium silicate-based root canal sealers accelerated setting time, decreased flow, and increased film thickness. However, the degree of these changes varied among the products. The present findings indicate that heat-induced changes in the physical properties of calcium silicate-based root canal sealers could adversely affect the quality of warm vertical condensation technique.

3.
Sci Rep ; 10(1): 20967, 2020 12 01.
Artigo em Inglês | MEDLINE | ID: mdl-33262423

RESUMO

When regenerative endodontic procedures (REPs) are performed on immature teeth diagnosed with pulp necrosis and apical periodontitis, various healing patterns occur. Furthermore, infected immature teeth with endodontic disorders often exhibit some remnant pulp and apical tissue. Therefore, this study investigated the impact of remnant healthy or fully functional pulp and apical tissue on healing patterns after REPs. Simulated REPs were performed on non-infected immature rat molars with different amounts of remnant pulp and apical tissue. Healing patterns in these teeth were assessed after 28 days. Teeth with 0.81-0.91 mm of remnant pulp healed with pulp-like tissue, dentin, and osteodentin-like dentin-associated mineralized tissue (OSD-DAMT); teeth with 0.60-0.63 mm of remnant pulp healed with pulp-like tissue and OSD-DAMT; teeth with 0.13-0.43 mm of remnant pulp healed with periodontal ligament (PDL)-like tissue, OSD-DAMT, and cementum-like dentin-associated mineralized tissue (CEM-DAMT); and teeth with disorganization of pulp and apical tissues at 0.15-0.38 mm beyond the root apex healed with PDL-like tissue, CEM-DAMT, and intracanal bone (IB). Loss of Hertwig's epithelial root sheath was observed with IB formation. These results showed that four distinct healing patterns occurred after REPs, depending on the preoperative amount of remnant healthy pulp and apical tissue.

4.
Dent Mater J ; 2020 Sep 29.
Artigo em Inglês | MEDLINE | ID: mdl-32999218

RESUMO

This study evaluated tristrontium aluminate (S3A) and its viability as a component for tricalcium silicate (C3S) cements. The properties of S3A, C3S, and S3A/C3S mixtures were evaluated in terms of setting time, compressive strength, flowability, and radiopacity. X-ray diffraction (XRD) pattern verified the powder synthesized in the laboratory as S3A, consequently, confirming the preparation method. S3A exhibited the lowest setting time, followed by C3S and S3A/C3S mixtures. Compressive strength of C3S was significantly higher than S3A. The S3A/C3S mixture showed comparable compressive strength to C3S for 1-day post initial mixing. There was no significant difference in flowability between S3A/C3S and mineral trioxide aggregate (MTA). S3A showed comparable radiopacity to MTA, whereas that of the S3A/C3S mixture was significantly lower comparatively; however, it achieved sufficient radiopacity (3 mm aluminum thickness equivalent). Further studies are needed to improve the manufacturing process of S3A and evaluate the bioactive effect of strontium.

5.
J Endod ; 2020 Sep 28.
Artigo em Inglês | MEDLINE | ID: mdl-33002538

RESUMO

INTRODUCTION: This study aimed to analyze torque/force generation and transportation in double-curved canals instrumented with 3 types of glide path files using optimum glide path (OGP) motion in comparison with continuous rotation. METHODS: Sixty simulated double-curved canals were prepared with #10/0.05 or #15/0.03 HyFlex EDM Glidepath files (Coltene/Whaledent, Altstätten, Switzerland) or a #13/0.04 prototype MANI Glidepath file (Tochigi, Japan) using OGP motion or continuous rotation (n = 10 each). Canals were sequentially prepared to 20 mm and 22 mm (full working length) using automated root canal instrumentation and a torque/force analyzing device. Transportation was calculated at 1-9 mm from the apex. Data were compared using 2-way analysis of variance followed by a post hoc simple main effect test with Bonferroni correction and a Kruskal-Wallis test (α = 5%). RESULTS: All #10/0.05 instruments fractured. In the 22-mm preparation, the OGP motion resulted in lower clockwise torque and screw-in force than did continuous rotation (P < .05). In the 20-mm preparation, #15/0.03 instruments recorded a lower screw-in force for OGP motion than for continuous rotation (P < .05). Comparing the 2 preparation phases, OGP motion generated no significant differences; however, continuous rotation developed higher clockwise torque and screw-in force in the 22-mm preparation than in the 20-mm preparation (P < .05). There was no significant difference among the tested groups for transportation values. CONCLUSIONS: Compared with continuous rotation, OGP motion generated less screw-in force, lower clockwise torque, and similar transportation. The #15/0.03 HyFlex EDM instrument and the #13/0.04 prototype MANI instrument performed similarly well.

6.
Materials (Basel) ; 13(20)2020 Oct 09.
Artigo em Inglês | MEDLINE | ID: mdl-33050334

RESUMO

Surface­reaction­type prereacted glass-ionomer (S­PRG) fillers exhibit bioactive properties by the release of multiple ions. This study examined whether a novel endodontic sealer containing S­PRG fillers (PRG+) has the capacity to induce osteoblast differentiation. Kusa­A1 osteoblastic cells were cultured with extracts of PRG+, PRG- (an experimental sealer containing S­PRG­free silica fillers), AH Plus (an epoxy-resin­based sealer), and Canals N (a zinc-oxide noneugenol sealer). Cell viability and mineralized nodule formation were determined using WST­8 assay and Alizarin red staining, respectively. Osteoblastic-marker expression was analyzed with RT­qPCR and immunofluorescence. Phosphorylation of extracellular signal­regulated kinase (ERK) and p38 mitogen­activated protein kinase (MAPK) was determined with Western blotting. Extracts of freshly mixed PRG+, PRG-, and AH Plus significantly decreased cell growth, but extracts of the set samples were not significantly cytotoxic. Set PRG+ significantly upregulated mRNAs for alkaline phosphatase and bone sialoprotein (IBSP) compared to set PRG-, and upregulation was blocked by NPS2143, a calcium­sensing receptor antagonist. Set PRG+ significantly accelerated IBSP expression, mineralized nodule formation, and enhanced the phosphorylation of ERK and p38 compared with set PRG-. In conclusion, PRG+ induced the differentiation and mineralization of Kusa­A1 cells via the calcium-sensing receptor-induced activation of ERK and p38 MAPK.

7.
Arch Oral Biol ; 119: 104881, 2020 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-32911120

RESUMO

OBJECTIVES: The present study aimed firstly, to investigate whether pulpal circulatory changes elicited by innocuous tooth cooling and foot heating can be monitored with transmitted-light plethysmography (TLP), which detects pulpal blood volume changes, and secondly, to assess the effect of autonomic nervous control on TLP values. DESIGN: Thirty sound permanent maxillary incisors in 30 healthy volunteers (age: 25-35 years) were examined. The photodiode and 525-nm light-emitting diode of a prototype TLP system (J. Morita) were fixed onto the palatal and labial side, respectively, of each tooth with a custom-made acrylic cap. The subjects were stimulated for 10 min by cold (0 °C) water application to the experimental tooth or 30 min by foot heating with a footbath (43 °C). TLP and finger plethysmography were simultaneously recorded before (baseline), during and after stimulation. TLP values and autonomic nerve activity were statistically analyzed using a repeated measures one-way ANOVA followed by Tukey post-hoc test and partial correlation analysis. RESULTS: TLP values decreased significantly after both innocuous stimuli (P < 0.05), and returned to their baseline levels shortly after the removal of the stimuli. There was no significant serial correlation between the autonomic nervous activity and TLP values (P > 0.05). CONCLUSION: TLP was able to monitor the pulpal circulatory changes evoked by innocuous stimuli. Systemic autonomic nervous control mechanisms were not associated with the pulpal circulatory changes, suggesting the involvement of other mechanisms, such as somatosensory-sympathetic nervous control.

8.
Am J Pathol ; 190(12): 2417-2426, 2020 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-32919979

RESUMO

Increased expression of the transient receptor potential ankyrin 1 (TRPA1) channel has been detected in carious tooth pulp, suggesting involvement of TRPA1 in defense or repair of this tissue after exogenous noxious stimuli. This study aimed to elucidate the induction mechanism in response to lipopolysaccharide (LPS) stimulation and the function of TRPA1 in dental pulp cells. Stimulation of human dental pulp cells with LPS up-regulated TRPA1 expression, as demonstrated by quantitative RT-PCR and Western blotting. LPS stimulation also promoted nitric oxide (NO) synthesis and p38/mitogen-activated protein kinase (MAPK) phosphorylation. NOR5, an NO donor, up-regulated TRPA1 expression, whereas 1400W, an inhibitor of inducible nitric oxide synthase, and SB202190, a p38/MAPK inhibitor, down-regulated LPS-induced TRPA1 expression. Moreover, JT010, a TRPA1 agonist, increased the intracellular calcium concentration and extracellular signal-regulated kinase 1/2 phosphorylation, and up-regulated alkaline phosphatase mRNA in human dental pulp cells. Icilin-a TRPA1 agonist-up-regulated secreted phosphoprotein 1 mRNA expression and promoted mineralized nodule formation in mouse dental papilla cells. In vivo expression of TRPA1 was detected in odontoblasts along the tertiary dentin of carious teeth. In conclusion, this study demonstrated that LPS stimulation induced TRPA1 via the NO-p38 MAPK signaling pathway and TRPA1 agonists promoted differentiation or mineralization of dental pulp cells.

9.
J Endod ; 2020 Aug 17.
Artigo em Inglês | MEDLINE | ID: mdl-32818563

RESUMO

INTRODUCTION: This study compared the static and dynamic cyclic fatigue resistance of contemporary nickel-titanium instruments with different kinematic, metallurgic, and design features to establish whether the fatigue-reducing effect of the pecking motion differs among different nickel-titanium instruments. METHODS: ProTaper Gold (PTG), Hyflex EDM (EDM), Reciproc Blue (RPB), and WaveOne Gold (WOG) files were divided into 2 groups of 10 for the static and dynamic cyclic fatigue resistance tests. A stainless steel artificial canal with 1.5-mm inner diameter, 60° angulation, and 3-mm radius of curvature was used. In the dynamic cyclic fatigue resistance test, speeds were set at 100 and 200 mm/min for the descending and ascending motion, respectively. The number of cycles to fracture (NCF) was calculated, the fractured lengths were recorded, and fractographic analysis of the fractured surfaces was carried out by scanning electron microscopy. Data were analyzed statistically with the Kruskal-Wallis test with Bonferroni correction (alpha = 0.05). RESULTS: The RPB and EDM showed significantly higher NCF in the static and dynamic cyclic fatigue resistance tests (P < .05). The dynamic cyclic fatigue resistance test showed significantly higher NCF than the static cyclic fatigue resistance test in the PTG and EDM (P < .05). There was no significant difference between the RPB and WOG (P > .05). CONCLUSIONS: In the experimental condition where the ascending speed was higher than the descending speed, the dynamic cyclic fatigue resistance was significantly higher than the static cyclic fatigue resistance in continuous rotary instruments, but not in reciprocating instruments.

10.
Photobiomodul Photomed Laser Surg ; 38(7): 438-443, 2020 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-32667863

RESUMO

Objective: This study aimed to examine the irrigant flow generated by laser-activated irrigation (LAI), in comparison with ultrasonic-activated irrigation (UAI) and syringe irrigation (SI), in the area beyond the ledge using particle image velocimetry (PIV). Background data: There was no reported study about cleaning efficacy of LAI beyond the ledge. Materials and methods: Forty-nine J-shaped root canal models (40° curvature) were instrumented to no. 35/0.06, and a ledge, 2.5 mm deep, was created with no. 60/0.08 instrument at 5 mm from the apical foramen in each canal. The samples were irrigated with LAI [30 mJ/5 pulse per second (pps), 30 mJ/10 pps, 30 mJ/20 pps, 50 mJ/10 pps, 70 mJ/10 pps], UAI, and SI with a tip/needle insertion depth of 5 mm from the apical foramen (n = 7). PIV was performed with glass beads and a high-speed camera. Velocities were compared in the coronal and apical areas to the ledge, respectively. Results: In the apical area, all LAI groups and UAI produced a higher velocity than that of SI, and LAI at 30 mJ/20 pps and 70 mJ/10 pps showed significantly higher velocity than that of UAI (p < 0.05). In the coronal area, LAI at 30 mJ/20 pps generated a significantly higher velocity than that of UAI and SI (p < 0.05). Velocity was significantly slower in the apical area than in the coronal area in UAI and SI (p < 0.05), but was similar between both areas in LAI except at 30 mJ/20 pps. Conclusions: Among tested laser settings, higher velocity was significantly achieved by LAI at 30 mJ/20 pps and 70 mJ/10 pps compared with UAI in the canal area beyond the ledge. SI generated lower fluid movement than LAI and UAI in both canal regions.

11.
J Oral Sci ; 62(3): 327-330, 2020 Jun 23.
Artigo em Inglês | MEDLINE | ID: mdl-32475865

RESUMO

This retrospective study aimed to investigate whether the corono-apical location of sinus tracts differs according to the presence/location of vertical root fracture (VRF) in microsurgically treated root-filled teeth. The cases included were (1) anterior and premolar teeth without a preoperative diagnosis of VRF, (2) those with a periodontal probing depth of ≤3 mm, and (3) those for which preoperative cone-beam computed tomography (CBCT) scans and intraoperative video records were available. VRF was diagnosed intraoperatively. The locations of buccal cortical bone defects and fracture lines were categorized on video images, and the corono-apical sinus tract locations were determined by superimposing video images onto volume-rendered CBCT images. Eleven of the 78 teeth investigated had VRF, and there was no significant difference in the incidence of sinus tracts between vertically fractured and non-fractured teeth (Mann-Whitney U-test, P > 0.05). The location of the sinus tract was significantly more coronal in vertically fractured than in non-fractured teeth (Mann-Whitney U-test, P < 0.0001). The location of sinus tracts was high correlated with cortical bone defects (Spearman's correlation, P < 0.0001). In microsurgically treated anterior and premolar teeth with a normal probing depth, sinus tracts were located more coronally in vertically fractured than in non-fractured teeth, and were highly correlated with the location of cortical bone defects.


Assuntos
Fraturas dos Dentes , Raiz Dentária , Tomografia Computadorizada de Feixe Cônico , Osso Cortical , Humanos , Microcirurgia , Estudos Retrospectivos
12.
J Endod ; 46(7): 973-979, 2020 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-32418616

RESUMO

INTRODUCTION: This ex vivo study aimed to evaluate the cleaning and shaping ability of a unique stainless steel rotary system (Gentlefile; MedicNRG, Kibbutz Afikim, Israel) compared with 2 nickel-titanium rotary instruments. METHODS: Thirty human mandibular premolars with a 15° to 25° curvature were equally distributed into 3 groups for final instrumentation with Gentlefile Red (#23/0.04), HyFlex EDM OneFile (#25/0.08∼; Coltene/Whaledent, Altstätten, Switzerland), and ProTaper Next X2 (#25/0.06v; Dentsply Sirona, Ballaigues, Switzerland) (n = 10/each). The untouched canal area, volume changes, and transportation were evaluated on pre- and post-instrumentation micro-computed tomographic images. Five random regions of the canal wall located 1-7 mm from the apical foramen were evaluated with scanning electron microscopy for superficial debris and a smear layer via a 5-point scoring system. Data were compared using the Kruskal-Wallis test with post hoc Dunn's pairwise comparison test with Bonferroni correction and Wilcoxon signed-rank test (α = 5%). RESULTS: All instruments generated no overt procedural errors. Untouched area and volume changes did not show any significant differences among the 3 groups (P > .05). The Gentlefile exhibited less transportation at the level of 5-7 mm from the apex compared with ProTaper Next (P < .05). The Gentlefile showed a smaller debris score than ProTaper Next and better smear layer removal than the others (P < .05). Complete cleanliness was not achieved by any of the systems investigated. CONCLUSIONS: Canals instrumented with the Gentlefile exhibited less transportation at the mid-root level and better cleanliness than those instrumented with HyFlex EDM and ProTaper Next.


Assuntos
Cavidade Pulpar , Preparo de Canal Radicular , Ligas Dentárias , Elétrons , Desenho de Equipamento , Humanos , Israel , Microscopia Eletrônica de Varredura , Titânio
14.
Photobiomodul Photomed Laser Surg ; 38(7): 431-437, 2020 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-32364877

RESUMO

Objective: Er:YAG laser-activated irrigation (LAI) is an effective method of root canal cleaning, but irrigant extrusion from the apical foramen has been a concern. We aimed to analyze the effects of pulse energy, pulse frequency, and laser tip diameter on intracanal vapor bubble kinetics and periapical pressure generation during LAI with Er:YAG laser. Background: Irrigant vapor bubble kinetics are one of indices of root canal cleaning efficacy. However, few studies have compared laser pulse conditions to vapor bubble kinetics, in relation to periapical pressure. Methods: A plastic root canal model (apical diameter 0.50 mm, 6% taper, 20 mm long) was filled with distilled water, and LAI with Er:YAG laser (Erwin AdvErl Unit; 30, 50, or 70 mJ; 10, or 20 pulses per second; laser tip R200T or R600T) was performed with the end of the tip fixed at 15 mm from the root apex. The number, maximum diameter, and velocity of vapor bubbles were analyzed by high-speed video imaging. Pressure generated outside the apical foramen was measured with a pressure sensor. Results: Vapor bubble count and maximum diameter increased significantly with pulse energy, pulse frequency, and tip diameter. Vapor bubble velocity increased significantly with pulse frequency, but not with pulse energy or tip diameter. Periapical pressure increased significantly with pulse energy, pulse frequency, and tip diameter. Conclusions: The pulse frequency was the single factor that significantly affected all the examined parameters (the number, diameter, and velocity) of vapor bubble kinetics together with the periapical pressure.

15.
J Endod ; 46(7): 943-949, 2020 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-32467021

RESUMO

INTRODUCTION: This study aimed to examine the process of reinnervation during coronal pulp tissue regeneration in a rat model in which rat bone marrow mesenchymal stem cells were implanted in pulpotomized molars. METHODS: The maxillary first molars of Wistar rats were pulpotomized, and preformed biodegradable porous poly L-lactic acid scaffolds and hydrogel carrying rat bone marrow mesenchymal stem cells were implanted in the pulp chamber. After 3, 7, and 14 days, the implanted teeth were processed for histologic analysis; immunoperoxidase staining for protein gene product 9.5 (a general neuronal marker), calcitonin gene-related peptide (CGRP), or substance P (SP); and real-time polymerase chain reaction for nerve growth factor (NGF) and growth-associated protein 43 (GAP-43) messenger RNA (mRNA) expression. RESULTS: Histologic analysis of the implanted region revealed sparse cellular distribution at 3 days, pulplike tissue with a thin dentin bridge-like structure at 7 days, and dentin bridge-like mineralized tissue formation and resorption of most scaffolds at 14 days. Protein gene product 9.5 and CGRP-immunoreactive nerve fibers showed the lowest density at 3 days and significantly increased until 14 days when the CGRP-immunoreactive fibers reached normal levels. SP-immunoreactive nerve fibers showed the highest density at 7 days and decreased to normal levels at 14 days. NGF mRNA increased with time, whereas GAP-43 mRNA levels peaked at 3 days and subsequently dropped until 14 days. CONCLUSIONS: Regeneration/remodeling of SP-immunoreactive and CGRP-immunoreactive nerve fibers with increased mRNA expression of NGF and GAP-43 occurred in a rat model of coronal pulp tissue engineering with bone marrow mesenchymal stem cells.


Assuntos
Polpa Dentária , Engenharia Tecidual , Animais , Dente Molar , Regeneração Nervosa , Ratos , Ratos Wistar
16.
Oral Dis ; 2020 Apr 05.
Artigo em Inglês | MEDLINE | ID: mdl-32248596

RESUMO

OBJECTIVES: We aimed to investigate whether the mesenchymal stem cell-endothelial cell crosstalk enhances angiogenic factor expression via nuclear factor-kappa B (NF-κB)-dependent mechanisms. MATERIALS AND METHODS: Human dermal microvascular endothelial cells (HDMECs) and stem cells from human exfoliated deciduous teeth (SHEDs) were cocultured for 96 hr, in the presence of NF-κB decoy oligodeoxynucleotides (ODNs) or scramble (control). Vascular endothelial cell growth factor (VEGF) and phospho-NF-κB p65 were measured with enzyme-linked immunosorbent assay. Angiogenesis-related gene expression was analyzed with microarray analysis followed by real-time polymerase chain reaction. Tube formation assay was conducted in the presence of NF-κB decoy. RESULTS: The VEGF and phospho-NF-κB p65 levels were significantly higher in the coculture with NF-κB decoy scramble than in single culture and coculture with NF-κB decoy ODN. Microarray analysis of SHEDs and HDMECs with NF-κB decoy scramble showed higher expression of proangiogenic genes, Bcl-2, NF-κB1, VEGFA, CXCL8, and CXCR1, and lower expression of proapoptotic genes, Bax and Caspase 9, compared to cells with NF-κB decoy ODN. Real-time PCR results for Bcl-2 and CXCL8 showed a similar trend. Tube formation assay showed more tube development in the presence of NF-κB decoy scramble. CONCLUSION: The SHED-HDMEC crosstalk enhanced proangiogenic factor expression via NF-κB-dependent pathways.

17.
J Endod ; 46(2): 232-237, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31889584

RESUMO

INTRODUCTION: Optimum torque reverse (OTR) motion is a torque-sensitive reciprocal motion in which the motor rotates in alternating 90° counterclockwise and 180° clockwise rotation when the torque exceeds a predetermined value. This study aimed to examine whether OTR motion contributes to torque and force reduction during nickel-titanium rotary instrumentation with the crown-down or single-length technique. METHODS: Twenty-eight simulated straight canals in resin blocks were divided into 2 groups according to the type of motion (OTR or continuous rotation). The groups were further subdivided according to the preparation technique (crown-down or single-length technique, n = 7 each). Automated root canal instrumentation was performed with a torque/force analyzing device (300 rpm, up-and-down speed of 10 mm/min) and EndoWave instruments (FKG Dentaire, La-Chaux-de-Fonds, Switzerland) to size #25/0.06 taper. Maximum torque and apical force were recorded and analyzed with analysis of variance and the Bonferroni test. RESULTS: During the crown-down preparation phase (#35/0.08, #30/0.06, #25/0.06, and #20/0.06), OTR motion developed lower maximum torque and upward force (representing the screw-in force) than continuous rotation. During the apical preparation phase (#25/0.06), OTR motion generated significantly lower maximum clockwise and counterclockwise torque (P < .05) when the single-length technique was used and significantly lower maximum upward force regardless of the preparation technique (P < .05) compared with continuous rotation. CONCLUSIONS: Under the present experimental conditions, OTR motion reduced both torque and screw-in force during the crown-down preparation phase of the crown-down technique and during the apical preparation phase of the single-length technique.


Assuntos
Cavidade Pulpar , Preparo de Canal Radicular , Coroas , Ligas Dentárias , Desenho de Equipamento , Rotação , Titânio , Torque
18.
Odontology ; 108(2): 188-193, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-31506734

RESUMO

This study aimed to analyze force/torque generation and canal volume changes of NiTi rotary glide path preparation using HyFlex EDM Glide Path File in comparison to manual stainless steel K-file instrumentation. Thirty extracted mandibular incisors with a minimally curved and narrow root canal were randomly divided into three groups (n = 10) according to the instrumentation kinematics: Optimum Glide Path motion (OGP) or continuous rotation (CR) with HyFlex EDM Glide Path Files using a custom-made automated-root-canal-preparation device and manual instrumentation with stainless steel K-files (SS) in watch-winding motion. Torque and force were monitored with a custom-made torque/force analyzing device. Canal volume changes and transportation values were measured on micro-computed tomographic images taken before and after the glide path preparation. The data were statistically evaluated using Kruskal-Wallis test and Mann-Whitney U test with Bonferroni correction, with a significance level set at 5%. Maximum upward apical force, representing the screw-in force, was lower in groups OGP and CR compared with that in group SS (P < 0.05). Group CR showed the highest maximum clockwise torque value and canal volume changes, followed by groups OGP and SS (P < 0.05). Canal transportation values at 1 and 3 mm from the apex were not significantly different among groups. Within the limitations of this study, rotary glide path preparation generated smaller screw-in force, larger torque and larger canal volume changes than manual preparation. OGP motion generated smaller torque and less canal volume changes than CR.


Assuntos
Níquel , Titânio , Ligas Dentárias , Cavidade Pulpar , Desenho de Equipamento , Dente Molar , Preparo de Canal Radicular , Torque
19.
Dent Mater J ; 39(2): 251-255, 2020 Mar 31.
Artigo em Inglês | MEDLINE | ID: mdl-31723092

RESUMO

This study aimed to evaluate the influence of a post-machining thermal treatment on canal-centering ability and torque/force generation of reciprocating nickel-titanium instruments. Simulated J-shaped resin canals were prepared with reciprocating instruments sharing identical geometric architecture and with/without post-machining thermal treatment (Reciproc Blue/Reciproc, VDW, Munich, Germany). Using an original automated root canal instrumentation and torque/force analyzing device, files were operated in a combination of reciprocation and up-and-down motion, and torque/force values were monitored. Canal-centering ratios were measured after superimposition of pre- and post-instrumentation images. Compared with Reciproc, Reciproc Blue showed a significantly lower canal-centering ratio (i.e., less deviation; p<0.05) at 0-1 mm from the apex and generated a significantly smaller upward maximum vertical force (p<0.05). Under standardized conditions using the automated device, Reciproc Blue showed better canal-centering ability and reduced screw-in forces than Reciproc, indicating that the post-machining thermal treatment confers superior performance to reciprocating nickel-titanium instruments.


Assuntos
Níquel , Titânio , Parafusos Ósseos , Instrumentos Odontológicos , Cavidade Pulpar , Desenho de Equipamento , Preparo de Canal Radicular
20.
Biochem Biophys Res Commun ; 522(2): 308-314, 2020 02 05.
Artigo em Inglês | MEDLINE | ID: mdl-31767145

RESUMO

Hypoxia-inducible factor 1 alpha (HIF1α) is a transcriptional factor that plays a key role in the regulation of various molecules expressed in hypoxic conditions. Ischemic/hypoxic conditions are regarded as a distinct characteristic of dental pulp inflammation due to the encasement of pulp tissue within the rigid tooth structure. This study was performed to examine the role of HIF1α in the regulation of interleukin (IL)-6, a proinflammatory cytokine expressed in inflamed dental pulp, in lipopolysaccharide (LPS)-stimulated human dental pulp cells (hDPCs). LPS stimulation promoted the expression of IL-6 in hDPCs, while HIF1α suppressed the expression of IL-6. Moreover, HIF1α induced suppressor of cytokine signaling 3 (SOCS3) expression in LPS-stimulated hDPCs, and SOCS3 activity led to downregulate expression of CCAAT enhancer-binding protein beta (CEBPß), an inducer of IL-6. LPS stimulation promoted HIF1α expression in hDPCs and mouse pulp tissue explants cultured under hypoxic conditions. These findings suggest that HIF1α negatively regulates IL-6 synthesis in LPS-stimulated hDPCs via upregulation of SOCS3 and subsequent downregulation of CEBPß.


Assuntos
Proteína beta Intensificadora de Ligação a CCAAT/metabolismo , Polpa Dentária/citologia , Subunidade alfa do Fator 1 Induzível por Hipóxia/metabolismo , Interleucina-6/biossíntese , Lipopolissacarídeos/farmacologia , Proteína 3 Supressora da Sinalização de Citocinas/metabolismo , Animais , Regulação para Baixo/efeitos dos fármacos , Humanos , Interleucina-6/genética , Camundongos , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , Regulação para Cima/efeitos dos fármacos
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