Your browser doesn't support javascript.
Mostrar: 20 | 50 | 100
Resultados 1 - 14 de 14
Filtrar
Mais filtros










Tipo de estudo
Intervalo de ano de publicação
1.
Artigo em Inglês | MEDLINE | ID: mdl-31781552

RESUMO

The potent immunomodulatory activities displayed by mesenchymal stromal cells (MSCs) have motivated their application in hundreds of clinical trials to date. In some countries, they have subsequently been approved for the treatment of immune disorders such as Crohn's disease and graft-versus-host disease. Increasing evidence suggests that their main mechanism of action in vivo relies on paracrine signaling and extracellular vesicles. Mesenchymal stromal cell-derived extracellular vesicles (MSC-EVs) play a prominent role in intercellular communication by allowing the horizontal transfer of microRNAs, mRNAs, proteins, lipids and other bioactive molecules between MSCs and their targets. However, despite the considerable momentum gained by MSC-EV research, the precise mechanism by which MSC-EVs interact with the immune system is still debated. Available evidence is highly context-dependent and fragmentary, with a limited number of reports trying to link their efficacy to specific active components shuttled within them. In this concise review, currently available evidence on the molecular mechanisms underlying the effects of MSC-EV cargo on the immune system is analyzed. Studies that pinpoint specific MSC-EV-borne mediators of immunomodulation are highlighted, with a focus on the signaling events triggered by MSC-EVs in target immune cells. Reports that study the effects of preconditioning or "licensing" in MSC-EV-mediated immunomodulation are also presented. The need for further studies that dissect the mechanisms of MSC-EV cargo in the adaptive immune system is emphasized. Finally, the major challenges that need to be addressed to harness the full potential of these signaling vehicles are discussed, with the ultimate goal of effectively translating MSC-EV treatments into the clinic.

2.
Stem Cells ; 37(10): 1357-1368, 2019 10.
Artigo em Inglês | MEDLINE | ID: mdl-31184411

RESUMO

Mesenchymal stromal cells (MSC) may exert their functions by the release of extracellular vesicles (EV). Our aim was to analyze changes induced in CD34+ cells after the incorporation of MSC-EV. MSC-EV were characterized by flow cytometry (FC), Western blot, electron microscopy, and nanoparticle tracking analysis. EV incorporation into CD34+ cells was confirmed by FC and confocal microscopy, and then reverse transcription polymerase chain reaction and arrays were performed in modified CD34+ cells. Apoptosis and cell cycle were also evaluated by FC, phosphorylation of signal activator of transcription 5 (STAT5) by WES Simple, and clonal growth by clonogenic assays. Human engraftment was analyzed 4 weeks after CD34+ cell transplantation in nonobese diabetic/severe combined immunodeficient mice. Our results showed that MSC-EV incorporation induced a downregulation of proapoptotic genes, an overexpression of genes involved in colony formation, and an activation of the Janus kinase (JAK)-STAT pathway in CD34+ cells. A significant decrease in apoptosis and an increased CD44 expression were confirmed by FC, and increased levels of phospho-STAT5 were confirmed by WES Simple in CD34+ cells with MSC-EV. In addition, these cells displayed a higher colony-forming unit granulocyte/macrophage clonogenic potential. Finally, the in vivo bone marrow lodging ability of human CD34+ cells with MSC-EV was significantly increased in the injected femurs. In summary, the incorporation of MSC-EV induces genomic and functional changes in CD34+ cells, increasing their clonogenic capacity and their bone marrow lodging ability. Stem Cells 2019;37:1357-1368.

3.
PLoS One ; 13(6): e0199332, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-29928002

RESUMO

Recently several studies demonstrated a role for the Wnt pathway in lymphocyte development and self-renewal of hematopoietic stem cells (HSCs). B-1 cells constitute a separate lineage of B lymphocytes, originating during fetal hematopoiesis, expressing lymphoid and myeloid markers and possessing self-renewal ability, similar to early hematopoietic progenitors and HSCs. A plethora of studies have shown an important role for the evolutionary conserved Wnt pathway in the biology of HSCs and T lymphocyte development. Our previous data demonstrated abundant expression of Wnt pathway components by B-1 cells, including Wnt ligands and receptors. Here we report that the canonical Wnt pathway is activated in B-1 cell precursors, but not in mature B-1 cells. However, both B-1 precursors and B-1 cells are able to respond to Wnt ligands in vitro. Canonical Wnt activity promotes proliferation of B-1 cells, while non-canonical Wnt signals induce the expansion of B-1 precursors. Interestingly, using a co-culture system with OP9 cells, Wnt3a stimulus supported the generation of B-1a cells. Taking together, these results indicate that B-1 cells and their progenitors are differentially responsive to Wnt ligands, and that the balance of activation of canonical and non-canonical Wnt signaling may regulate the maintenance and differentiation of different B-1 cell subsets.


Assuntos
Linfócitos B/citologia , Linfócitos B/metabolismo , Células-Tronco/metabolismo , Via de Sinalização Wnt , Animais , Linfócitos B/efeitos dos fármacos , Antígenos CD5/metabolismo , Proliferação de Células/efeitos dos fármacos , Feminino , Interleucina-7/farmacologia , Ligantes , Camundongos Endogâmicos C57BL , Receptores de Interleucina-7/metabolismo , Células-Tronco/citologia , Células-Tronco/efeitos dos fármacos , Proteínas Wnt/farmacologia , Via de Sinalização Wnt/efeitos dos fármacos
4.
Braz. j. microbiol ; 47(3): 770-774, July-Sept. 2016. graf
Artigo em Inglês | LILACS | ID: lil-788952

RESUMO

ABSTRACT The objective of this study was to characterize genotypically Malassezia spp. isolated from the external ear canal of healthy horses. Fifty-five horses, 39 (70.9%) males and 16 (29.1%) females, from different breeds and adults were studied. External ear canals were cleaned and a sterile cotton swab was introduced to collect cerumen. A total of 110 samples were cultured into Dixon medium and were incubated at 32 °C for up to 15 days. Macro- and micromorphology and phenotypic identification were performed. DNA was extracted, strains were submitted to polymerase chain reaction technique, and the products obtained were submitted to Restriction Fragment Length Polymorphism using the restriction enzymes BstCI and HhaI. Strains were sent off to genetic sequencing of the regions 26S rDNA D1/D2 and ITS1-5.8S-ITS2 rDNA. Malassezia spp. were isolated from 33/55 (60%) animals and 52/110 (47%) ear canals. No growth on Sabouraud dextrose agar was observed, confirming the lipid dependence of all strains. Polymerase chain reaction-Restriction fragment length polymorphism permitted the molecular identification of Malassezia nana - 42/52 (81%) and Malassezia slooffiae - 10/52 (19%). Sequencing confirmed RFLP identification. It was surprising that M. nana represented over 80% of the strains and no Malassezia equina was isolated in this study, differing from what was expected.


Assuntos
Animais , Masculino , Feminino , Meato Acústico Externo/microbiologia , Microbiota , Cavalos/microbiologia , Malassezia/isolamento & purificação , Malassezia/classificação , Polimorfismo de Fragmento de Restrição , Reação em Cadeia da Polimerase , Genes Bacterianos , Malassezia/genética
5.
Braz J Microbiol ; 47(3): 770-4, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-27287335

RESUMO

The objective of this study was to characterize genotypically Malassezia spp. isolated from the external ear canal of healthy horses. Fifty-five horses, 39 (70.9%) males and 16 (29.1%) females, from different breeds and adults were studied. External ear canals were cleaned and a sterile cotton swab was introduced to collect cerumen. A total of 110 samples were cultured into Dixon medium and were incubated at 32°C for up to 15 days. Macro- and micromorphology and phenotypic identification were performed. DNA was extracted, strains were submitted to polymerase chain reaction technique, and the products obtained were submitted to Restriction Fragment Length Polymorphism using the restriction enzymes BstCI and HhaI. Strains were sent off to genetic sequencing of the regions 26S rDNA D1/D2 and ITS1-5.8S-ITS2 rDNA. Malassezia spp. were isolated from 33/55 (60%) animals and 52/110 (47%) ear canals. No growth on Sabouraud dextrose agar was observed, confirming the lipid dependence of all strains. Polymerase chain reaction-Restriction fragment length polymorphism permitted the molecular identification of Malassezia nana - 42/52 (81%) and Malassezia slooffiae - 10/52 (19%). Sequencing confirmed RFLP identification. It was surprising that M. nana represented over 80% of the strains and no Malassezia equina was isolated in this study, differing from what was expected.


Assuntos
Meato Acústico Externo/microbiologia , Cavalos/microbiologia , Malassezia/classificação , Malassezia/isolamento & purificação , Microbiota , Animais , Feminino , Genes Bacterianos , Malassezia/genética , Masculino , Reação em Cadeia da Polimerase , Polimorfismo de Fragmento de Restrição
6.
Immunobiology ; 220(1): 60-7, 2015 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-25245014

RESUMO

The Wnt/ß-catenin signaling pathway has been shown to play an important role in controlling the proliferation, survival and differentiation of hematopoietic cells. Several Wnt/ß-catenin signaling components influence hematopoietic cells fate. B-1 cells are self-renewing and spontaneously express both myeloid and lymphoid restricted transcription factors. B-1 lymphocytes play a major role in autoimmunity and are related to CD5(+) B-cell lymphomas and leukemias, such as CLL (chronic lymphocytic leukemia). Herein, we demonstrate that Wnt/ß-catenin pathway is important to B-1 cell survival in vitro. The loss of Wnt signals by quercetin treatment induces a reduction in the proliferation and survival of B-1 cells. Furthermore, the quercetin treatment diminishes IL-6 production by peritoneal cells, a cytokine important to the maintenance of B-1 cells in vitro. Importantly, the IL-6 addition to B-1 cell culture prevents cells from apoptosis, even in the presence of quercetin. These data suggest that a deregulation in ß-catenin signals could result in alterations in B-1 cell proliferation and differentiation. The correlation between Wnt/ß-catenin and IL-6 could point out a mechanism for the expansion of B-1 cells in autoimmune disease and neoplasia.


Assuntos
Quercetina/farmacologia , Via de Sinalização Wnt/efeitos dos fármacos , Animais , Subpopulações de Linfócitos B/efeitos dos fármacos , Subpopulações de Linfócitos B/metabolismo , Técnicas de Cultura de Células , Linhagem Celular , Proliferação de Células/efeitos dos fármacos , Sobrevivência Celular/efeitos dos fármacos , Feminino , Interleucina-6/metabolismo , Camundongos
7.
BMC Microbiol ; 14: 299, 2014 Dec 20.
Artigo em Inglês | MEDLINE | ID: mdl-25527183

RESUMO

BACKGROUND: Attachment is essential to maintain bacteria at their preferential intestinal colonization sites. There is little information on the influence of different environmental conditions in the interaction of atypical enteropathogenic Escherichia coli (aEPEC) strains with epithelial cells. In this study, we evaluated the effect of different glucose (5 and 25 mM) and CO2 (0.03 and 5%) concentrations and presence of bile salts on the adhesiveness of the aEPEC strain 1551-2. RESULTS: We found that a CO2-enriched atmosphere enhanced the adhesiveness of the aEPEC 1551-2 strain independently of glucose concentrations or presence of bile salts. Conversely, the presence of high glucose concentration altered the original localized adherence (LA) pattern observed at 5 mM glucose, which is characterized by the formation of compact bacterial clusters, to a hybrid adherence pattern (LA and an aggregative adherence-like pattern). In addition, at high glucose concentration, there was increased expression of the fimA gene, which encodes the major subunit of type 1 pilus (T1P), and an isogenic fimA mutant displayed only LA. The presence of bile salts did not interfere with the adhesion properties of the 1551-2 strain to HeLa cells. CONCLUSIONS: Our data suggest that a CO2-enriched atmosphere could favor aEPEC adhesion to the host cells, whereas enhanced T1P production under high glucose concentration could allow bacteria to access more extensive intestinal colonization sites in the host at the beginning of the infectious process.


Assuntos
Aderência Bacteriana , Escherichia coli Enteropatogênica/fisiologia , Exposição Ambiental , Células Epiteliais/microbiologia , Interações Hospedeiro-Patógeno , Ácidos e Sais Biliares/metabolismo , Dióxido de Carbono/metabolismo , Glucose/metabolismo , Células HeLa , Humanos
8.
Immunobiology ; 219(6): 403-15, 2014 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-24594322

RESUMO

Macrophages respond to endogenous and non-self stimuli acquiring the M1 or M2 phenotypes, corresponding to classical or alternative activation, respectively. The role of B-1 cells in the regulation of macrophage polarization through the secretion of interleukin (IL)-10 has been demonstrated. However, the influence of B-1 cells on macrophage phenotype induction by an immunogen that suppress their ability to secrete IL-10 has not been explored. Here, we studied the peritoneal macrophage pattern induced by liposomes comprised of dipalmitoylphosphatidylcholine (DPPC) and cholesterol (Chol) carrying ovalbumin (OVA) (Lp DPPC/OVA), and the involvement of B-1 cells in macrophage polarization. Peritoneal cells from BALB/c, B-1 cells-deficient BALB/xid and C57BL/6 mice immunized with Lp DPPC/OVA and OVA in soluble form (PBS/OVA) were analyzed and stimulated or not in vitro with lipopolysaccharide (LPS). Peritoneal macrophages from BALB/c and C57BL/6 mice immunized with Lp DPPC/OVA showed an M2-like phenotype as evidenced by their high arginase activity without LPS stimulation. Upon stimulation, these macrophages were reprogrammable toward the M1 phenotype with the upregulation of nitric oxide (NO) and a decrease in IL-10 secretion. In addition, high IFN-γ levels were detected in the culture supernatant of peritoneal cells from BALB/c and C57BL/6 mice immunized with Lp DPPC/OVA. Nevertheless, still high levels of arginase activity and undetectable levels of IL-12 were found, indicating that the switch to a classical activation state was not complete. In the peritoneal cells from liposomes-immunized BALB/xid mice, levels of arginase activity, NO, and IL-6 were below those from wild type animals, but the last two products were restored upon adoptive transfer of B-1 cells, together with an increase in IFN-γ secretion. Summarizing, we have demonstrated that Lp DPPC/OVA induce an M2-like pattern in peritoneal macrophages reprogrammable to M1 phenotype after LPS stimulation, with the involvement of B-1 cells.


Assuntos
Linfócitos B/imunologia , Colesterol/farmacologia , Lipossomos/farmacologia , Macrófagos Peritoneais/imunologia , Ovalbumina/farmacologia , 1,2-Dipalmitoilfosfatidilcolina/farmacologia , Transferência Adotiva , Animais , Arginase/biossíntese , Linfócitos B/transplante , Proliferação de Células/efeitos dos fármacos , Células Cultivadas , Portadores de Fármacos/farmacologia , Interferon gama/biossíntese , Interleucina-10/metabolismo , Interleucina-12/biossíntese , Interleucina-6/biossíntese , Lipopolissacarídeos/imunologia , Ativação de Macrófagos/imunologia , Macrófagos Peritoneais/citologia , Masculino , Camundongos , Camundongos Endogâmicos BALB C , Camundongos Endogâmicos C57BL , Camundongos Transgênicos , Óxido Nítrico/biossíntese , Fenótipo , Fosfatidilcolinas/farmacologia
9.
Artigo em Inglês | MEDLINE | ID: mdl-23431344

RESUMO

The present study analyzed the immune modulation mechanisms of thymulin 5CH in a granuloma experimental model. Male adult Balb/c mice were inoculated with BCG into the footpad to induce granuloma, which was quantitatively evaluated. The phenotypic characterization of phagocyte, T- and B-lymphocyte populations in the peritoneum, and local lymph node was done by flow cytometry. During all experimental periods, thymulin 5CH and vehicle (control) were given ad libitum to mice, diluted into the drinking water (1.6 × 10(-17) M). After 7 days from inoculation, thymulin-treated mice presented reduction in the number of epithelioid cytokeratine-positive cells (P = 0.0001) in the lesion, in relation to young phagocytes. After 21 days, the differentiation of B1 peritoneal stem cells into phagocytes reached the peak, being higher in thymulin-treated mice (P = 0.0001). Simultaneously, the score of infected phagocytes in the lesion decreased (P = 0.001), and the number of B1-derived phagocytes, CD4+ and CD8+ T lymphocytes in the local lymph node increased in relation to control (P = 0.0001). No difference was seen on the CD25+ Treg cells. The results show that thymulin 5CH treatment is able to improve the granuloma inflammatory process and the infection remission, by modulating local and systemic phagocyte differentiation.

10.
J Vet Diagn Invest ; 24(5): 1014-6, 2012 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-22826042

RESUMO

Diseases caused by extraintestinal pathogenic Escherichia coli (ExPEC) in wild felids are rarely reported. Although urinary tract infections are infrequently reported in domestic cats, such infections when present are commonly caused by ExPEC. The present work characterized ExPEC strains isolated from 2 adult felines, a snow leopard (Panthera uncia) and a black leopard (Panthera pardus melas), that died from secondary bacteremia associated with urinary tract infections. Isolates from both animals were classified into the B2 phylogenetic group and expressed virulence genotypes that allowed them to cause severe disease. In addition, strains from the black leopard showed multidrug resistance.


Assuntos
Bacteriemia/veterinária , Infecções por Escherichia coli/veterinária , Felidae , Infecções Urinárias/veterinária , Animais , Animais de Zoológico , Antibacterianos/uso terapêutico , Bacteriemia/microbiologia , Enrofloxacina , Infecções por Escherichia coli/microbiologia , Evolução Fatal , Feminino , Fluoroquinolonas/uso terapêutico , Masculino , Infecções Urinárias/tratamento farmacológico , Infecções Urinárias/microbiologia , Infecções Urinárias/patologia
11.
PLoS One ; 7(3): e34570, 2012.
Artigo em Inglês | MEDLINE | ID: mdl-22479646

RESUMO

The inflammatory response is driven by signals that recruit and elicit immune cells to areas of tissue damage or infection. The concept of a mononuclear phagocyte system postulates that monocytes circulating in the bloodstream are recruited to inflamed tissues where they give rise to macrophages. A recent publication demonstrated that the large increase in the macrophages observed during infection was the result of the multiplication of these cells rather than the recruitment of blood monocytes. We demonstrated previously that B-1 cells undergo differentiation to acquire a mononuclear phagocyte phenotype in vitro (B-1CDP), and we propose that B-1 cells could be an alternative origin for peritoneal macrophages. A number of recent studies that describe the phagocytic and microbicidal activity of B-1 cells in vitro and in vivo support this hypothesis. Based on these findings, we further investigated the differentiation of B-1 cells into phagocytes in vivo in response to LPS-induced inflammation. Therefore, we investigated the role of B-1 cells in the composition of the peritoneal macrophage population after LPS stimulation using osteopetrotic mice, BALB/Xid mice and the depletion of monocytes/macrophages by clodronate treatment. We show that peritoneal macrophages appear in op/op((-/-)) mice after LPS stimulation and exhibit the same Ig gene rearrangement (VH11) that is often found in B-1 cells. These results strongly suggest that op/op((-/-)) peritoneal "macrophages" are B-1CDP. Similarly, the LPS-induced increase in the macrophage population was observed even following monocyte/macrophage depletion by clodronate. After monocyte/macrophage depletion by clodronate, LPS-elicited macrophages were observed in BALB/Xid mice only following the transfer of B-1 cells. Based on these data, we confirmed that B-1 cell differentiation into phagocytes also occurs in vivo. In conclusion, the results strongly suggest that B-1 cell derived phagocytes are a component of the LPS-elicited peritoneal macrophage population.


Assuntos
Subpopulações de Linfócitos B/imunologia , Lipopolissacarídeos/imunologia , Macrófagos Peritoneais/imunologia , Fagócitos/imunologia , Animais , Subpopulações de Linfócitos B/citologia , Diferenciação Celular , Células Cultivadas , Genes de Imunoglobulinas , Inflamação/imunologia , Macrófagos Peritoneais/citologia , Macrófagos Peritoneais/metabolismo , Camundongos , Camundongos Endogâmicos BALB C , Camundongos Endogâmicos C57BL , Fagócitos/citologia , Fagócitos/metabolismo
12.
Int. j. high dilution res ; 10(36): 194-195, september 30, 2011.
Artigo em Inglês | LILACS-Express | ID: hom-10705

RESUMO

Em estudos prévios, foi verificado que a timulina (um hormônio tímico), quando preparada em potências homeopáticas 5cH, tem a propriedade de melhorar a performance produtiva de frangos infectados com reovirus, bem como modular o desenvolvimento do tumor de Ehrlich e de lesões granulomatosas em camundongos por mecanismos imunomediados. O objetivo deste trabalho foi estudar os mecanismos imunonodulatórios da timulina 5cH em um modelo de granuloma experimental, pela injeção subcutânea de BCG em camundongos, focando o envolvimento das células B1 e do zinco no processo. Três grupos de camundongos Balb/c machos (grupo A tratado com timulina 5cH, grupo B tratado com timulina 5cH incubada com Chelex ® - um quelante de zinco ? e grupo C, controle, tratado com veículo) foram inoculados com BCG no coxim plantar esquerdo e o granuloma subcutâneo e baço foram colhidos para análise histomorfométrica, após 7, 14 e 21 dias. Colorações de Ziehl-Neelsen, hematoxilina eosina e azul da Prússia foram também utilizadas. A citometria de fluxo também foi usada nos mesmos tempos para caracterizar e quantificar células peritoneais. Células positivas para CD11b (fagócitos ativados, células B1), CD 19 (B1 e B2), CD23 (negativas em B1, positivas em B2) e CD5 (B1a) foram analisadas em um aparelho FACS Calibur (BD). A análise estatística foi Kruskal-Wallis/Dunn para avaliações não paramétricas e ANOVA/Tuckey-Krammer para avaliações paramétricas. O método X2 foi usado para avaliar a proporção entre células na citometria. Valores de p?0.05 foram considerados estatisticamente significantes. Camundongos tratados com timulina 5cH apresentaram alta atividade de macrófagos e aumento na área folicular esplênica após 7 dias da inoculação do BCG. O aumento do tamanho macroscópico da lesão e a redução da infecção local foram vistos após 21 dias. Nesse período, a citometria demonstrou aumento na proporção de fagócitos derivados de células B1 peritoneais em camundongos tratados com timulina 5cH, independentemente da incubação ou não com Chelex®, a qual bloqueou apenas os efeitos da timulina sobre células B2 no peritôneo e reduziu significativamente os níveis de manganês na preparação medicamentosa. Conclui-se que a timulina 5cH modula o granuloma induzido por BCG através de mais de um mecanismo, especialmente da diferenciação de células B1 peritoneais em fagócitos.(AU)


In previous studies, we found that thymulin (a thymic hormone), when prepared in homeopathic 5cH potency, had the property to improve the productive performance of broiler chickens infected with reovirus, as well as modulate the development of Ehrlich tumor and granuloma inflammatory lesions in mice by immune-mediated mechanisms. The aim of the present work was to study the immunomodulatory mechanisms of thymulin 5cH in a granuloma experimental model, by subcutaneous inoculation of BCG in mice, focusing the B-1 cells and zinc involvement in this process. Three groups of male Balb/c SPF mice (group A treated with thymulin 5cH, group B treated with thymulin 5cH incubated in Chelex ® - a zinc chelant - and group C, control, treated with vehicle) were inoculated with BCG in the left footpad and subcutaneous granuloma and spleen were harvested for histomorphometry analysis, after 7, 14 and 21 days. Ziehl-Neelsen, HE and Prussia Blue staining methods were used. Flow cytometry was also used in the same times to characterize and quantify peritoneal cells. Positive cells for CD11b (activated phagocytes, B-1 cells), CD19 (B-1 and B-2 cells), CD23 (negative B-1 cells, positive B2 cells) and CD5 (B-1a cells) were analyzed in a FACS Calibur (BD) device. Statistical analysis was performed using Kruskal - Wallis / Dunn for nonparametric evaluations and ANOVA / Tuckey-Krammer for the parametric ones. The X² method was used to evaluate the cell count in flow cytometry. P values ? 0.05 were considered statistically significant. Mice treated with thymulin 5cH presented higher macrophage activity and increase in the follicular area were seen in spleen after 7 days. Increase in gross lesion diameter and decrease in local BCG infection were seen after 21 days. At this time, the flow cytometry demonstrated the increase in peritoneal phagocytes derived from B-1 cells in thymulin 5cH treated mice, independently of Chelex ® incubation. The incubation of thymulin 5cH with Chelex ® blocked its effects only upon the number of B2 cells in the peritoneum and reduces Mn levels in the medicine solution. We conclude that thymulin 5CH modulates the BCG-induced granuloma through more than one mechanism, especially by peritoneal B1 cell differentiation into phagocytes.(AU)


Assuntos
Hormônios do Timo , Granuloma , Mycobacterium bovis , Linfócitos B , Zinco
13.
Int. j. high dilution res ; 8(27): 41-44, 2009. tab, graf
Artigo em Inglês | LILACS | ID: lil-529835

RESUMO

This paper reports the results of incubation of a strain of uropathogenic Escherichia coli (UPEC) isolated from a snow leopard - which had died of septicemia secondary to necro-hemorrhagic cystitis - with homeopathic and isopathic remedies. Methods: UPEC was isolated from heart blood and previously typified for virulence factors; it was incubated with homeopathic remedies Cantharis vesicatoria (urinary tract infection affinity), Mercurius solubilis (from symptoms analysis) and nosode prepared from the actual strain, all in dilution 12cH. Results: 2 patterns of bacterial growth were observed, associated to the quality of nutrients in the culture medium; inrich-nutrient medium, nosode of E. coli 12cH had a significant inhibitory effect; in poor-nutrient medium, Merc 12cH exerted significant inhibitory effect. Conclusion: results suggest that the previous conditions of prokaryote systems may influence the in vitro response to homeopathic and isopathic remedies.


Este artigo relata os resultados da incubação de uma linhagem de Escherichia coli uropatogênica (UPEC) isolada a partir de um leopardo das neves, que morreu de septicemia secundária a cistite necrótica-hemorrágica. A UPEC foi tratada com preparados homeopáticos e isopáticos. Métodos: UPEC foi isolada de sangue cardíaco e previamente tipificada para fatores de virulência; foi incubada com o medicamento homeopático Cantharis vesicatoria (afinidade com infecção do trato urinário), Mercurius solubilis (a partir da análise de sintomas) e nosódio preparado a partir da mesma linhagem de bactérias, todas em 12 cH. Resultados: 2 padrões de crescimento bacteriano foram observados, associados à qualidade dos nutrientes do meio de cultura; em meios ricos em nutrientes, nosódio de E. coli 12 cH teve um significativo efeito inibitório; em meio pobre de nutrientes, Merc 12 cH exerceu efeito inibitório significativo. Conclusão: os resultados sugerem que as condições prévias do sistema procarioto estudado podem influenciar as respostas proliferativas in vitro para preparados homeopáticos e isopáticos.


Assuntos
Escherichia , Felidae , Homeopatia , Infecções Urinárias , Isoterapia
14.
Int. j. high dilution res ; 8(27): 41-44, 2009. tab, graf
Artigo em Inglês, Português | HomeoIndex - Homeopatia | ID: hom-9216

RESUMO

This paper reports the results of incubation of a strain of uropathogenic Escherichia coli (UPEC)isolated from a snow leopard - which had died of septicemia secondary to necro-hemorrhagiccystitis - with homeopathic and isopathic remedies. Methods: UPEC was isolated from heartblood and previously typified for virulence factors; it was incubated with homeopathic remediesCantharis vesicatoria (urinary tract infection affinity), Mercurius solubilis (from symptomsanalysis) and nosode prepared from the actual strain, all in dilution 12cH. Results: 2 patterns ofbacterial growth were observed, associated to the quality of nutrients in the culture medium; inrich-nutrient medium, nosode of E. coli 12cH had a significant inhibitory effect; in poor-nutrientmedium, Merc 12cH exerted significant inhibitory effect. Conclusion: results suggest that theprevious conditions of prokaryote systems may influence the in vitro response to homeopathic andisopathic remedies.(AU)


Este artigo relata os resultados da incubação de uma linhagem de Escherichia coli uropatogênica (UPEC) isolada a partir de um leopardo das neves, que morreu de septicemia secundária a cistite necrótica-hemorrágica. A UPEC foi tratada com preparados homeopáticos e isopáticos. Métodos: UPEC foi isolada de sangue cardíaco e previamente tipificada para fatores de virulência; foi incubada com o medicamento homeopático Cantharis vesicatoria (afinidade com infecção do trato urinário), Mercurius solubilis (a partir da análise de sintomas) e nosódio preparado a partir da mesma linhagem de bactérias, todas em 12 cH. Resultados: 2 padrões de crescimento bacteriano foram observados, associados à qualidade dos nutrientes do meio de cultura; em meios ricos em nutrientes, nosódio de E. coli 12 cH teve um significativo efeito inibitório; em meio pobre de nutrientes, Merc 12 cH exerceu efeito inibitório significativo. Conclusão: os resultados sugerem que as condições prévias do sistema procarioto estudado podem influenciar as respostas proliferativas in vitro para preparados homeopáticos e isopáticos. (AU)


Assuntos
Infecções Urinárias , Felidae , Homeopatia , Isoterapia , Escherichia
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA