Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 10 de 10
Filtrar
Mais filtros










Base de dados
Intervalo de ano de publicação
1.
Nutr Cancer ; 72(3): 504-512, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-31290695

RESUMO

Although several studies have investigated the cytotoxic effects of different Fabaceae species, limited researches have been conducted on the cytotoxic effect of Dorycnium pentaphyllum. The aim of this study was to evaluate the phenolic characterization and the cytotoxic effect of D. pentaphyllum on human cervix (HeLa) and colon (WiDr) cancer cells and the possible mechanisms involved. Total phenolic content (TPC) and phenolic characterization of the extract were investigated using the Folin-Cioceltau method and RP-HPLC, respectively. The cytotoxic effect of the extract was evaluated using the MTT assay. The mechanism involved in the extract's cytotoxic effect was then evaluated in terms of apoptosis and the cell cycle using flow cytometry, while mitochondrial membrane potential (MMP) was investigated using the fluorometric method. The TPC value of the extract was 141.2 ± 0.8 mg gallic acid equivalent per g sample, and quercetin was detected as major phenolics. D. pentaphyllum extract exhibited a selective cytotoxic effect on HeLa and WiDr cells compared to normal fibroblast and colon cells, respectively. The extract induced cell cycle arrest at the S phase and apoptosis via reduced MMP in these cells. Further studies may be useful in developing a natural product based new generation pharmacological agent.

2.
Histol Histopathol ; 34(4): 431-443, 2019 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-30394506

RESUMO

Following an injury or resection, the mammalian liver has the capacity to regain its former volume and functioning by restoring itself. Studies have demonstrated that antioxidants play a role in hepatic regeneration. This study investigated the effect of 4-(3,4-dihydroxybenzoyloxymethyl) phenyl-O-ß-D-glucopyranoside (PG) obtained from Origanum micranthum on liver regeneration. Sixty Wistar Albino rats were used. In the sham-operated group, a midline abdominal laparotomy was performed without hepatectomy. In the partial hepatectomy (PHx) group, the median and left lateral lobes were removed. Rats in the PHx group received 20 mg/kg/day PG intraperitoneally before being sacrificed at 24, 48, and 72 hrs, and 7 days later. Liver tissues were collected for immunohistochemical analysis and electron microscopic evaluation. We found an increase in mitotic index, and the numbers of Ki-67 stained hepatocytes in all PHx early stage groups (24 hr, 48hr, 72 hr), but not in 7-day groups. The regeneration mediators eNOS, iNOS, TNF-α and NF-κB were shown to increase in PHx groups. This increase was more prominent dependening on time. In the PHx treatment (PHx+PG) groups, while eNOS was still high, iNOS, TNF-α and NF-κB had decreased. The apoptotic index was markedly high in the PHx groups; this was prevented by PG treatment. These findings were supported by the ultrastructural results. Our findings indicate that PG supports liver regeneration, hepatocyte proliferation, reduced liver damage, and inflammatory mediators following PHx.


Assuntos
Proliferação de Células/efeitos dos fármacos , Regeneração Hepática/efeitos dos fármacos , Metilglucosídeos/farmacologia , Animais , Hepatectomia , Hepatócitos/efeitos dos fármacos , Masculino , Ratos , Ratos Wistar
3.
Chem Biodivers ; 14(6)2017 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-28207990

RESUMO

Plant phenolics are known to display many pharmacological activities. In the current study, eight phenolic compounds, e.g., luteolin 5-O-ß-glucoside (1), methyl rosmarinate (2), apigenin (3), vicenin 2 (4), lithospermic acid (5), soyasaponin II (6), rubiadin 3-O-ß-primeveroside (7), and 4-(ß-d-glucopyranosyloxy)benzyl 3,4-dihydroxybenzoate (8), isolated from various plant species were tested at 0.2 mm against carbonic anhydrase-II (CA-II) and urease using microtiter assays. Urease inhibition rate for compounds 1 - 8 ranged between 5.0 - 41.7%, while only compounds 1, 2, and 4 showed a considerable inhibition over 50% against CA-II with the IC50 values of 73.5 ± 1.05, 39.5 ± 1.14, and 104.5 ± 2.50 µm, respectively, where IC50 of the reference (acetazolamide) was 21.0 ± 0.12 µm. In silico experiments were also performed through two docking softwares (Autodock Vina and i-GEMDOCK) in order to find out interactions between the compounds and CA-II. Actually, compounds 6 (30.0%) and 7 (42.0%) possessed a better binding capability toward the active site of CA-II. According to our results obtained in this study, among the phenolic compounds screened, particularly 1, 2, and 4 appear to be the promising inhibitors of CA-II and may be further investigated as possible leads for diuretic, anti-glaucoma, and antiepileptic agents.


Assuntos
Inibidores da Anidrase Carbônica/farmacologia , Fenóis/farmacologia , Urease/antagonistas & inibidores , Domínio Catalítico , Simulação por Computador , Simulação de Acoplamento Molecular , Extratos Vegetais/química , Extratos Vegetais/farmacologia , Ligação Proteica , Relação Estrutura-Atividade
4.
Chem Pharm Bull (Tokyo) ; 63(9): 720-5, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-26329865

RESUMO

Thymus praecox ssp. grossheimii (RONNIGER) JALAS var. grossheimii (Lamiaceae) is used as an herbal tea for cold, stomachache, cough, and infections in Turkey. There are no phytochemical studies on this species. We performed phytochemical studies and quantitative analysis of rosmarinic acid and luteolin 5-O-ß-D-glucopyranoside in the methanol extract of the plant. Several chromatographic methods were used for the isolation of major compounds. HPLC methods were applied for quantitative analysis of rosmarinic acid and luteolin 5-O-ß-D-glucopyranoside in the methanol extract. In this study, ursolic acid (1), oleanolic acid (2), methyl rosmarinate (3), ethyl rosmarinate (4), rosmarinic acid (5), luteolin 5-O-ß-D-glucopyranoside (6), and thymoquinol 2,5-O-ß-diglucopyranoside (7) were isolated from the aerial parts of the plant. The relative contents of rosmarinic acid and luteolin 5-O-ß-D-glucopyranoside in the extract were 15.2 and 57.8 mg/g of dry weight, respectively. Compounds isolated from this plant and the contents of rosmarinic acid and luteolin 5-O-ß-D-glucopyranoside provided reasonable evidence for the traditional usages of this plant.


Assuntos
Cinamatos/análise , Depsídeos/análise , Flavonas/análise , Glucosídeos/análise , Compostos Fitoquímicos/análise , Thymus (Planta)/química , Cromatografia Líquida de Alta Pressão , Conformação Molecular
5.
J Pak Med Assoc ; 64(3): 260-5, 2014 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-24864596

RESUMO

OBJECTIVE: To investigate the potential protective effects of Rosmarinic acid (RA) on rats exposed to ischaemia/reperfusion renal injury. METHODS: The prospective study was conducted at Abant Izzet Baysal University, Turkey, and comprised 21 male Spraque Dawley rats weighing 250-270g each. They were divided into three equal groups. Unilaterally nephrectomised rats were subjected to 60 minutes of left renal ischaemia followed by 60 minutes of reperfusion. Group 1 had sham-operated animals; group 2 had ischaemia/reperfusion untreated animals; and group 3 had ischaemia/reperfusion animals treated with rosmarinic acid. Serum creatinine, blood urea nitrogen, tissue malondialdehyde, glutathione peroxidase, superoxide dismutase and myeloperoxidase (MPO) activities, and light microscopic findings were evaluated. SPSS 17 was used for statistical analysis. RESULTS: Treatment of rats with rosmarinic acid produced a reduction in the serum levels of creatinine and blood urea nitrogen compared to the other groups. However, no statistically significant difference was found. The levels of malondialdehyde and myeloperoxidase were decreased in the renal tissue of group 3, while glutathione peroxidose and superoxide dismutase levels remained unchanged. The injury score decreased in the treatment group rats compared to the untreated group. Rosmarinic acid significantly decreased focal glomerular necrosis, dilatation of Bowman's capsule, degeneration of tubular epithelium, necrosis in tubular epithelium, and tubular dilatation. CONCLUSIONS: Rosmarinic acid prevented ischaemia/reperfusion injury in the kidneys by decreasing oxidative stress.


Assuntos
Cinamatos/farmacologia , Depsídeos/farmacologia , Nefropatias/sangue , Nefropatias/prevenção & controle , Traumatismo por Reperfusão/sangue , Traumatismo por Reperfusão/prevenção & controle , Animais , Nitrogênio da Ureia Sanguínea , Creatinina/sangue , Masculino , Malondialdeído/sangue , Nefrectomia , Estresse Oxidativo , Peroxidase/sangue , Estudos Prospectivos , Ratos , Ratos Sprague-Dawley
6.
Adv Clin Exp Med ; 21(4): 433-9, 2012.
Artigo em Inglês | MEDLINE | ID: mdl-23240448

RESUMO

BACKGROUND: Intestinal ischemia and reperfusion (I-R) injury of different causes, including cardiac insufficiency, sepsis, vasodepressant and cardiodepressant drugs, and complications of long-lasting surgery, represents a major clinical problem. OBJECTIVES: The purpose of the present study was to investigate whether lithospermic acid (LA) can reduce oxidative stress and histological damage in the rat small bowel subjected to mesenteric I-R injury. MATERIAL AND METHODS: The study was performed on three groups of animals, each composed of 7 rats: the SO (sham operation) group, the I-R/Untreated group and the I-R/LA (I-R plus LA pretreatment) group. Intestinal ischemia for 45 minutes and reperfusion for 60 minutes were applied. Ileum specimens were obtained to determine the tissue level of malondialdehyde (MDA), superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase (CAT) and myeloperoxidase (MPO) activities and histological changes. RESULTS: Untreated intestinal I-R resulted in increased tissue MDA and MPO levels and diminished SOD and GPx activities. These changes were found to be almost reversed in the LA treatment group. Histopathologically, the intestinal injury in rats treated with LA was less than the untreated I-R group. CONCLUSIONS: Lithospermic acid attenuates mesenteric ischemia reperfusion injury in rat intestines by increasing tissue SOD and GPx activities and decreasing MDA and MPO levels. Lithospermic acid also improves morphological alterations which occurred after periods of reperfusion.


Assuntos
Benzofuranos/farmacologia , Depsídeos/farmacologia , Intestino Delgado/irrigação sanguínea , Traumatismo por Reperfusão/prevenção & controle , Animais , Catalase/metabolismo , Glutationa Peroxidase/metabolismo , Intestino Delgado/enzimologia , Intestino Delgado/metabolismo , Malondialdeído/metabolismo , Peroxidase/metabolismo , Ratos , Ratos Sprague-Dawley , Superóxido Dismutase/metabolismo
7.
Molecules ; 15(4): 2593-9, 2010 Apr 12.
Artigo em Inglês | MEDLINE | ID: mdl-20428066

RESUMO

Sonchus erzincanicus (Asteraceae) is an endemic species in Turkey, where six Sonchus species grow. In this study, a phytochemical study was performed on the aerial parts of the plant. The study describes the isolation and structure elucidation of five flavonoids and two a-ionone glycosides from S. erzincanicus. The compounds were isolated using several and repeated chromatographic techniques from ethyl acetate and aqueous phases that were partitioned from a methanol extract obtained from the plant. 5,7,3',4'-Tetrahydroxy-3-methoxyflavone (1) and quercetin 3-O-beta-D-glucoside (2) were isolated from the ethyl acetate phase, while corchoionoside C 6'-O-sulfate (3), corchoionoside C (4), luteolin 7-O-glucuronide (5) and luteolin 7-O-beta-D-glucoside (6), apigenin 7-O-glucuronide (7) were isolated from the aqueous phase. Corchoionoside C 6'-O-sulfate (3), isolated for the first time from a natural source, was a new compound. The structures of the compounds were elucidated by means of 1H-NMR, 13C-NMR, 2D-NMR (COSY, HMQC, HMBC) and ESI-MS.


Assuntos
Glucosídeos/química , Norisoprenoides/química , Sonchus/química , Ésteres do Ácido Sulfúrico/química , Flavonoides/química , Flavonoides/isolamento & purificação , Glucosídeos/isolamento & purificação , Espectroscopia de Ressonância Magnética , Norisoprenoides/isolamento & purificação , Espectrometria de Massas por Ionização por Electrospray , Ésteres do Ácido Sulfúrico/isolamento & purificação
8.
Mycoses ; 52(2): 135-40, 2009 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-18522697

RESUMO

Methanol and chloroform extracts obtained from eight plant species belonging to six families, which were selected depending on their use in Turkish folk medicine, including Mentha longifolia L. (Labiatae), Mentha piperita L. Hudson (Labiatae), Prongos ferulaceae (Umbelliferae), Galium verum L. (Rubiaceae), Salvia limbata C. A Meyer (Labiatae), Artemisia austriaca Jacq. (Artemiceae), Plantago lanceolata L. (Plantaginaceae) and Urtica dioica L. (Urticaceae) were evaluated for their in vitro anticandidal activity. The anticandidal activity of extracts against 99 human pathogenic clinical isolates belonging to 35 Candida albicans, 33 Candida tropicalis and 31 Candida glabrata and standard strains of Candida spp. (C. albicans ATCC 10231, C. glabrata ATCC 80030 and C. tropicalis ATCC 22019) were tested by disc diffusion method and the active extracts were assayed for the minimal inhibitory concentration (MIC). Chloroform extracts of plants have no inhibitory effect against both clinical and standard strains of Candida spp., whereas methanol extracts exhibited good activity. Among the plants tested, M. piperita showed the highest anticandidal activity with 12.3 mm inhibition zone and 1.25 mg ml(-1) MIC value against C. albicans, M. longifolia, P. lanceolata and A. austriaca also displayed activity against C. albicans and C. tropicalis.


Assuntos
Candida , Magnoliopsida/química , Medicina Tradicional , Extratos Vegetais/farmacologia , Plantas Medicinais/química , Candida/classificação , Candida/efeitos dos fármacos , Candida albicans/efeitos dos fármacos , Candidíase/microbiologia , Humanos , Magnoliopsida/classificação , Testes de Sensibilidade Microbiana , Fitoterapia , Turquia
9.
Chem Biol Interact ; 170(1): 40-8, 2007 Oct 20.
Artigo em Inglês | MEDLINE | ID: mdl-17681286

RESUMO

This study investigated the effects of Onosma armeniacum K. (Boraginaceae) root extract (AR-1) on ethanol-induced stomach ulcers, and on some oxidant and antioxidant parameters, in stomach tissue in rats. The results obtained showed that AR-1 significantly inhibited ethanol-induced ulcers at 25, 50, 100 and 200 mg/kg doses. We found that 50, 100 and 200 mg/kg doses of AR-1 inhibited ulcers more effectively than did ranitidine. AR-1 at doses of 25, 50, 100 and 200 mg/kg significantly prevented the decrease in total glutathione (tGSH) level which occurs in damaged stomach tissues of rats given ethanol (control group). Only a 100 mg/kg dose of AR-1 significantly increased the glutathione S-transferase (GST) level in stomach tissue compared to the control. All doses of AR-1 except the 25 mg/kg dose eliminated the decrease in the superoxide dismutase (SOD) level in the stomach tissue of rats given ethanol. While all doses of AR-1 decreased malondialdehyde (MDA) levels significantly; all doses AR-1 except 25 mg/kg decreased myeloperoxidase (MPO) levels significantly compared to the control. The effect of AR-1 on catalase (CAT) activity was insignificant at all doses. AR-1 significantly increased nitric oxide (NO) levels at 50, 100 and 200 mg/kg doses compared to the control. Our results indicate that the protection of some antioxidant mechanisms and the inhibition of some oxidant mechanisms have a role in AR-1's antiulcer effect mechanism.


Assuntos
Boraginaceae/química , Fitoterapia/métodos , Extratos Vegetais/farmacologia , Úlcera Gástrica/tratamento farmacológico , Estômago/efeitos dos fármacos , Animais , Catalase/metabolismo , Etanol/toxicidade , Mucosa Gástrica/metabolismo , Glutationa/metabolismo , Glutationa Transferase/metabolismo , Ácido Linoleico/metabolismo , Peroxidação de Lipídeos/efeitos dos fármacos , Malondialdeído/metabolismo , Óxido Nítrico/metabolismo , Estresse Oxidativo/efeitos dos fármacos , Peroxidase/metabolismo , Raízes de Plantas/metabolismo , Ranitidina/farmacologia , Ratos , Ratos Wistar , Estômago/enzimologia , Úlcera Gástrica/induzido quimicamente , Úlcera Gástrica/enzimologia , Úlcera Gástrica/metabolismo , Superóxido Dismutase/metabolismo , Substâncias Reativas com Ácido Tiobarbitúrico/metabolismo
10.
Biol Pharm Bull ; 27(5): 702-5, 2004 May.
Artigo em Inglês | MEDLINE | ID: mdl-15133249

RESUMO

Antioxidant and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activities, reducing powers and the amount of total phenolic compounds of aqueous and/or methanolic extracts of some medicinal plants used in Eastern Turkey were studied. These plants are Prangos ferulacea (CASIR), Sedum sempervivoides (HOROZ LELESI), Malva neglecta (EBEMGUMECI), Cruciata taurica (SARILIK OTU), Rosa pimpinellifolia (KOYUN GOZU), Galium verum subsp. verum (MADAVUR OTU), Urtica dioica (ISIRGAN). The highest peroxidation inhibitions were shown by aqueous extracts of C. taurica and R. pimpinellifolia (IC(50): 0.00022 mg/l and IC(50): 23 mg/l, respectively). However, the highest DPPH radical scavenging activity, reducing power and the amount of phenolic compounds were shown by R. pimpinellifolia. The lowest antioxidant properties were shown by aqueous extract of M. neglecta.


Assuntos
Antioxidantes/farmacologia , Extratos Vegetais/farmacologia , Plantas Medicinais , Antioxidantes/isolamento & purificação , Apiaceae , Crassulaceae , Depuradores de Radicais Livres/farmacologia , Peroxidação de Lipídeos/efeitos dos fármacos , Peroxidação de Lipídeos/fisiologia , Malvaceae , Extratos Vegetais/isolamento & purificação , Rosaceae , Rubiaceae , Urtica dioica
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA