Your browser doesn't support javascript.
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 94
Filtrar
Mais filtros










Intervalo de ano de publicação
1.
Sci Rep ; 10(1): 784, 2020 Jan 21.
Artigo em Inglês | MEDLINE | ID: mdl-31964947

RESUMO

IL-17A is an important pro-inflammatory cytokine involved in the inflammatory response in chronic obstructive pulmonary disease (COPD). To evaluate the role played by single nucleotide polymorphisms of IL17A and protein levels in susceptibility to COPD, 1,807 subjects were included in a case-control study; 436 had COPD related to tobacco smoking (COPD-S) and 190 had COPD related to biomass burning (COPD-BB). Six hundred fifty-seven smokers without COPD (SWOC) and 183 biomass burning-exposed subjects (BBES) served as the respective control groups. The CC genotype and C allele of rs8193036 were associated with COPD (COPD-S vs. SWOC: p < 0.05; OR = 3.01, and OR = 1.28, respectively), as well as a recessive model (p < 0.01; OR = 2.91). Significant differences in serum levels were identified between COPD-S vs. SWOC, COPD-S vs. COPD-BB, and SWOC vs. BBES (p < 0.01). By comparing genotypes in the COPD-BB group TT vs. CC and TC vs. CC (p < 0.05), we found lower levels for the CC genotype. Logistic regression analysis by co-variables was performed, keeping the associations between COPD-S vs. SWOC with both polymorphisms evaluated (p < 0.05), as well as in COPD-BB vs. BBES but with a reduced risk of exacerbation (p < 0.05). In conclusion, polymorphisms in IL17A are associated with COPD. Serum levels of IL-17A were higher in smokers with and without COPD.

2.
Arch Bronconeumol ; 2019 Nov 26.
Artigo em Inglês, Espanhol | MEDLINE | ID: mdl-31784347

RESUMO

INTRODUCTION: It is unclear whether low-risk patients with acute symptomatic pulmonary embolism (PE) should undergo echocardiogram. METHODS: We performed a meta-analysis of studies that enrolled patients with acute low-risk PE to assess the prognostic value of echocardiographic diagnosis of right ventricular (RV) dysfunction for the primary outcome of short-term all-cause mortality, and the secondary outcome of short-term PE-related mortality. We used a random-effects model to pool study results, a Begg rank correlation method to evaluate for publication bias, and I2 testing to assess heterogeneity. RESULTS: The meta-analysis included a total of 11 studies 1,868 patients with low-risk PE. Ten of the 447 (2.2%; 1.1%-4.1%) low-risk patients with echocardiographic RV dysfunction died soon after the diagnosis of PE compared with 10 of 1,421 (0.7%; 0.3-1.3%) patients without RV dysfunction. RV dysfunction was not significantly associated with short-term all-cause mortality (odds ratio 2.0; 95% confidence interval, 0.8-5.1, p=.14; I2=8%). RV dysfunction was significantly associated with short-term PE-related mortality (odds ratio 5.2; 95% confidence interval, 1.7-16, p <.01; I2=0%). CONCLUSIONS: In patients with low-risk PE, echocardiographic RV dysfunction is not associated with all-cause mortality, but identifies patients with an increased risk for short-term PE-related mortality.

3.
Ther Adv Drug Saf ; 10: 2042098619868640, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31632633

RESUMO

Polypharmacy and fall-risk increasing drugs (FRIDS) have been associated with injurious falls. However, no information is available about the association between FRIDS and injurious falls after hospital discharge due to hip fracture in a very old population. We aim to assess the association between the use of FRIDS at discharge and injurious falls in patients older than 80 years hospitalized due to a hip fracture. A retrospective cohort study using routinely collected health data will be conducted at the Orthogeriatric Unit of a teaching hospital. Patients will be included at hospital discharge (2014), with a 2-year follow-up. Fall-risk increasing drugs will be recorded at hospital discharge, and exposure to drugs will be estimated from usage records during the 2-year follow-up. Injurious falls are defined as falls that lead to any kind of health care (primary or specialized care, including emergency department visits and hospital admissions). A sample size of 193 participants was calculated, assuming that 40% of patients who receive any FRID at discharge, and 20% who do not, will experience an injurious fall during follow up. This protocol explains the study methods and the planned analysis. We expect to find a relevant association between FRIDS at hospital discharge and the incidence of injurious falls in this very old, high risk population. If confirmed, this would support the need for a careful pharmacotherapeutic review in patients discharged after a hip fracture. However, results should be carefully interpreted due to the risk of bias inherent to the study design.

4.
J Ayub Med Coll Abbottabad ; 31(3): 297-298, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31535494

RESUMO

Different diagnostic and treatment algorithms for prosthetic infections of the hip and knee are available and widely used in North America. However, for the best of our knowledge, the sampling methodology in Latin American countries is not protocolized varying among the members of the region. In conclusion, we recommend that samples should aim to screen for aerobic, anaerobic, mycobacterial, fungal, and intracellular bacteria. These recommendations are based on experience, especially in Latin America. Further research is necessary for the realization of an international consensus.

5.
Pap. psicol ; 40(2): 109-124, mayo-ago. 2019. graf, tab
Artigo em Espanhol | IBECS | ID: ibc-183641

RESUMO

El presente estudio es una revisión sistemática de las investigaciones realizadas sobre los factores de riesgo y los factores protectores relacionados con el ciberbullying entre adolescentes. La búsqueda se realizó en las bases de datos Web of Science, PsycARTICLES, EBSCOhost, Science Direct Journals, Scopus y Springer Journals. Luego de aplicar los criterios de inclusión a los 226 artículos encon-trados inicialmente, se tuvo como resultado un total de 39 artículos que conformaron la muestra final. El N total de las muestras fue 173.179 adolescentes. La investigación con la muestra más pequeña tuvo 90 participantes y la muestra más grande estuvo conforma-da por 72.327 personas. Los factores de riesgo y los factores protectores están relacionados con el uso de Internet y las Tecnologías de la Información y la Comunicación, aspectos familiares, sociales, psicológicos e individuales. La información consignada en esta re-visión sistemática deja ver la importancia de programas de intervención que prevengan y atiendan el ciberbullying, en los que se in-volucre a la familia, la escuela y la sociedad


The present study is a systematic review of the research on the risk and protective factors related to cyberbullying among adolescents. The research was carried out using the databases Web of Science, PsycARTICLES, EBSCOhost, Science Direct journals, Scopus and Springer journals. Applying the inclusion criteria to the 226 articles initially found, resulted in a total of 39 articles that made up the final sample. The total N of the samples was 173,179 adolescents. The research with the smallest sample had 90 participants and the largest sample was made up of 72,327 participants. The risk and protective factors are related to the use of the Internet and information and communication technologies, family, social, psychological and individual aspects. The information contained in this systematic review shows the importance of intervention programs that prevent and address cyberbullying, involving family, school and society


Assuntos
Humanos , Adolescente , Bullying/psicologia , Fatores de Risco , Fatores de Proteção , Comportamento do Adolescente/psicologia , Psicologia do Adolescente , Internet , Mídias Sociais/estatística & dados numéricos
6.
Reumatol Clin ; 2019 Jun 28.
Artigo em Inglês, Espanhol | MEDLINE | ID: mdl-31262506

RESUMO

BACKGROUND: Patients with RA have a two to four-fold increased risk of developing infections compared to the general population. For this reason, the administration of influenza, pneumococcal and shingles vaccines is recommended for all patients with RA, preferably prior to initiating treatment, Previous studies have demonstrated the low prevalence of vaccination as well as adherence to current recommendations by rheumatologists in other regions. OBJECTIVE: To determine the knowledge and adherence to the current vaccination recommendations for patients with RA by rheumatology members of the Mexican College of Rheumatology (MCR), and to identify barriers to their application in this population. METHODS: A cross-sectional study was conducted through a survey sent to 577 rheumatologists from Mexico in January 2017. RESULTS: We received completed surveys from 122 individuals, representing 21.14% of the 577 rheumatologists in our registry. Fifty percent responded that they recommended immunization against influenza to 76%-100% of their patients, 36.07% recommended immunization against pneumococcus to 76%-100% of their patients, and 69.67% of the survey responders did not recommend shingles immunization routinely to their patients. CONCLUSIONS: The data collected in this study show there is poor adherence to immunization schedules recommended for the RA population. This data suggests there is misinformation about the effectiveness, safety and optimal timing of immunization in patients with RA in Mexico.

8.
Org Lett ; 21(13): 5346-5350, 2019 Jul 05.
Artigo em Inglês | MEDLINE | ID: mdl-31247753

RESUMO

Complex CpRuCl(PPh3)2 catalyzes reactions of terminal alkynes with 4-picoline N-oxide and primary and secondary amines to afford the corresponding amides. The reactions occur in chlorinated solvent and aqueous medium, showing applications in peptide chemistry. Stoichiometric studies reveal that the true catalysts of the processes are the vinylidene cations [CpRu(═C═CHR)(PPh3)2]+ which are oxidized to the Ru(η2-CO)-ketenes by the N-oxide. Finally, nucleophilic additions of primary and secondary amines to the free ketenes yield the corresponding amides.

9.
Cuad. psicol. deporte ; 19(2): 12-27, mayo 2019. tab, graf
Artigo em Espanhol | IBECS | ID: ibc-183266

RESUMO

El propósito de este trabajo es doble. Por un lado, pretende describir los aspectos motivacionales y beneficios que obtienen los exdeportistas de élite que se mantienen físicamente activos después de la retirada y las barreras que perciben los exdeportistas físicamente inactivos, comparando los niveles de actividad física que reportan ambos grupos. Por otro lado, probar las relaciones entre los diferentes tipos de motivos y regulaciones conductuales que pueden predecir la práctica de actividad física saludable y deporte recreativo de los exdeportistas de élite. Se realizó un estudio transversal. Participaron 317 exdeportistas de élite (Medad = 36.5, DS = 7.18 años) completaron un cuestionario en línea que evaluaba motivos, regulaciones conductuales, cantidad de ejercicio, estados de cambio, beneficios y barreras. Respecto a la regulación conductual, los exdeportistas mostraron altos niveles de motivación intrínseca y regulación identificada y bajos de regulación extrínseca y amotivación. Los beneficios principales de practicar actividad física saludable y deporte recreativo fueron la diversión, la revitalización y la buena salud. Los exdeportistas físicamente inactivos (12.9%) detectaron como barreras principales los conflictos con otras actividades, su forma física actual y el hecho de haber perdido acceso a recursos e instalaciones. Los exdeportistas físicamente activos mostraron un perfil con niveles más altos de motivos intrínsecos, regulación interna y actividad física que los inactivos. Por su parte, los inactivos reportaron niveles significativamente más altos de motivos extrínsecos y amotivación. Proponemos que para promover actividad física saludable y deporte recreativo en la población de exdeportistas inactivos los programas de promoción deberían enfatizar la buena salud, el reconocimiento social y la competición, motivos todos ellos que predicen positivamente la regulación intrínseca y la actividad física autoinformada


The purpose of this study is twofold: (a) to describe the motivational aspects and gains obtained by physically active former elite athletes andthe barriers experienced by inactive former elite athletes comparing their reported exercise amount, and (b)to testwhether the different motivationswould predict their reported exercise amount. Using a cross-sectional survey design, 317 former athletes (Mage = 36.5, SD = 7.18 years) completed an online questionnaire assessing motivation, self-reported exercise and sport practice, stages of change, gains, and barriers. The main gains reported by former athletes were enjoyment, revitalization and positive health. Physically inactive former athletes (12.9%) spotted as the main barriers conflict with other activities, current physical fitness and lost accessto facilities and resources. To promote healthy physical activity in the physically inactive population of former elite athletes, policy makers and physical activity organizers should emphasize positive health, social recognition and competition motives, which positively predict intrinsic regulation and vigorous physical activity


O objetivo deste artigo é duplo: (a) descrever os aspectos motivacionais e benefícios obtidos por exdeportistas elite é fisicamente ativo e barreiras percebidas por fisicamente inativos, e (b) a testar um modelo motivacional que pode prever prática de atividade física nesta população. Um estudo transversal foi realizado. Havia 317 ex-atletas de elite (Medad = 36,5, DS = 7,18 anos). Eles preencheram um questionário on-line de motivações, quantidade de exercício, estados de mudança, benefícios e barreiras. Os principais benefícios foram diversão, revitalização eboa saúde. As principais barreiras para os inativos (12,9%) foram os conflitos com outras atividades, sua forma física atual e o fato de ter perdido acesso a recursos e instalações. Para promover a atividade física saudável na população de ex-atletas inativos, os programas de promoção devem enfatizar a boa saúde, o reconhecimento social e a competição, que preveem positivamente a regulação intrínseca e a atividade física


Assuntos
Humanos , Masculino , Feminino , Adulto Jovem , Adulto , Pessoa de Meia-Idade , Atletas/estatística & dados numéricos , Exercício/psicologia , Técnicas de Exercício e de Movimento/estatística & dados numéricos , Estilo de Vida Saudável , Motivação , Condicionamento Físico Humano/estatística & dados numéricos , Autonomia Pessoal
10.
Cyberpsychol Behav Soc Netw ; 22(5): 349-354, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-30896977

RESUMO

Problematic Internet use (PIU) is a growing clinical concern to clinicians working in adolescent mental health, with significant potential comorbidities like depression and substance use. No prior study has examined associations between PIU, high-risk behavior, and psychiatric diagnoses specifically in psychiatrically hospitalized adolescents. Here, we analyzed how PIU severity correlated with preadmission Internet habits, psychiatric symptoms, and high-risk behavior in this unique population. We hypothesized that as the severity of PIU increased, so would endorsement of mood symptoms, engagement in risky behaviors, and chances of having comorbid mood and aggression-related diagnoses. We performed a cross-sectional survey on an adolescent psychiatric inpatient unit in an urban community hospital in Massachusetts. Participants were 12-20 years old (n = 205), 62.0 percent female, and of diverse racial/ethnic backgrounds. Relationships between PIU, high-risk symptoms, diagnoses, and behaviors were performed both using chi-square tests and determining Pearson correlation coefficients. Two hundred five adolescents participated in the study. PIU severity was associated with being female (p < 0.005), sexting (p < 0.05), cyberbullying (p < 0.005), and increased suicidality within the last year (p < 0.05). Adolescents with aggressive and developmental disorders, but not depressive disorders, also had significantly higher PIU scores (p ≤ 0.05). In our sample of psychiatrically hospitalized adolescents, PIU severity was significantly associated with both serious psychiatric symptoms and high-risk behaviors, including those related to suicide. Our findings may improve safety assessments in this vulnerable adolescent population by identifying comorbid risks associated with problematic digital media use.


Assuntos
Comportamento do Adolescente/psicologia , Adolescente Institucionalizado/psicologia , Transtorno Depressivo/psicologia , Internet/estatística & dados numéricos , Assunção de Riscos , Adolescente , Adolescente Institucionalizado/estatística & dados numéricos , Criança , Estudos Transversais , Diagnóstico Duplo (Psiquiatria)/psicologia , Feminino , Humanos , Masculino , Suicídio , Inquéritos e Questionários , Adulto Jovem
11.
Rev Invest Clin ; 71(1): 7-16, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-30810545

RESUMO

Background: Smoking is considered an epidemic, indeed, one of the most important public health problems worldwide. It is also the most significant preventable cause of death, of a high number of premature deaths, and avoidable chronic diseases. It is considered an enormous economic burden for the world. Objective: To provide an overview of smoking-cessation treatments, including pharmacological and psychological options, and to gather current scientific evidence available on them. Methods: Research included reviewing publications from 2007-2018 in four databases using algorithms related to bupropion, varenicline, nicotine replacement therapy, smoking cessation, psychological treatment, motivational interview, cognitive-behavioral therapy and clinical guidelines for smoking treatment. Meta-analyses or systematic reviews and randomized or quasi-randomized trials were selected. We also included clinical guidelines for smoking treatment from Mexico and other countries. Results: After refining the search, 37 articles met the criteria and were included in the review. The results were grouped by type of intervention. Conclusions: It is necessary to conduct research on combinations of both kinds of treatment with an integral, multidisciplinary vision. Current standard for smoking cessation is a combined psychological and pharmacological treatment.


Assuntos
Guias de Prática Clínica como Assunto , Abandono do Hábito de Fumar/métodos , Dispositivos para o Abandono do Uso de Tabaco , Bupropiona/administração & dosagem , Terapia Cognitivo-Comportamental/métodos , Humanos , México , Entrevista Motivacional/métodos , Ensaios Clínicos Controlados Aleatórios como Assunto , Fumar/efeitos adversos , Fumar/epidemiologia , Abandono do Hábito de Fumar/psicologia , Agentes de Cessação do Hábito de Fumar/administração & dosagem , Vareniclina/administração & dosagem
12.
Toxicol Appl Pharmacol ; 369: 30-38, 2019 Apr 15.
Artigo em Inglês | MEDLINE | ID: mdl-30763598

RESUMO

Despite all the development of modern medicine, around 100 compounds derived from natural products were undergoing clinical trials only at the end of 2013. Among these natural substances in clinical trials, we found the resveratrol (RES), a pharmacological multi-target drug. RES analgesic properties have been demonstrated, although the bases of these mechanisms have not been fully elucidated. The aim of this study was to evaluate the involvement of opioid and cannabinoid systems in RES-induced peripheral antinociception. Paw withdrawal method was used and hyperalgesia was induced by carrageenan (200 µg/paw). All drugs were given by intraplantar injection in male Swiss mice (n = 5). RES (100 µg/paw) administered in the right hind paw induced local antinociception that was antagonized by naloxone, non-selective opioid receptor antagonist, and clocinnamox, µOR selective antagonist. Naltrindole and nor-binaltorfimine, selective antagonists for δOR and kOR, respectively, did not reverse RES-induced peripheral antinociception. CB1R antagonist AM251, but not CB2R antagonist AM630, antagonized RES-induced peripheral antinociception. Peripheral antinociception of RES intermediate-dose (50 µg/paw) was increased by: (i) bestatin, inhibitor of endogenous opioid degradation involved-enzymes; (ii) MAFP, inhibitor of anandamide amidase; (iii) JZL184, inhibitor of 2-arachidonoylglycerol degradation involved-enzyme; (iv) VDM11, endocannabinoid reuptake inhibitor. Acute and peripheral administration of RES failed to affect the amount of µOR, CB1R and CB2R. Experimental data suggest that RES induces peripheral antinociception through µOR and CB1R activation by endogenous opioid and endocannabinoid releasing.

13.
Mar Pollut Bull ; 140: 462-471, 2019 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-30803667

RESUMO

Desalination has the potential to provide an important source of potable water to growing coastal populations but it also produces highly saline brines with chemical additives, posing a possible threat to benthic marine communities. The effects of brine (0%, 50%, 100%) were compared to seawater treatments with the same salinity (37, 46, 54 psu) for seagrass (Posidonia australis) in mesocosms over 2 weeks. There were significant differences between brine and salinity treatments for photosynthesis, water relations and growth. Germinating seedlings of P. australis were also tested in brine treatments (0%, 25%, 50%, 100%) over 7 weeks followed by 2.5 weeks recovery in seawater. Growth was severely inhibited only in 100% brine. These experiments demonstrated that brine increased the speed and symptoms of stress in adult plants compared to treatments with the same salinity, whereas seedlings tolerated far longer brine exposure, and so could potentially contribute to seagrass recovery through recruitment.


Assuntos
Alismatales/efeitos dos fármacos , Sais/toxicidade , Água do Mar/química , Plântula/efeitos dos fármacos , Poluentes Químicos da Água/toxicidade , Alismatales/crescimento & desenvolvimento , Alismatales/fisiologia , Austrália , Biomassa , Monitoramento Ambiental , Modelos Teóricos , Salinidade , Sais/química , Plântula/crescimento & desenvolvimento , Plântula/fisiologia , Poluentes Químicos da Água/química
14.
Ageing Res Rev ; 49: 27-48, 2019 01.
Artigo em Inglês | MEDLINE | ID: mdl-30391755

RESUMO

INTRODUCTION: We aimed to perform a review of SRs of non-pharmacological interventions in older patients with well-defined malnutrition using relevant outcomes agreed by a broad panel of experts. METHODS: PubMed, Cochrane, EMBASE, and CINHAL databases were searched for SRs. Primary studies from those SRs were included. Quality assessment was undertaken using Cochrane and GRADE criteria. RESULTS: Eighteen primary studies from seventeen SRs were included. Eleven RCTs compared oral nutritional supplementation (ONS) with usual care. No beneficial effects of ONS treatment, after performing two meta-analysis in body weight changes (six studies), mean difference: 0.59 (95%CI -0.08, 1.96) kg, and in body mass index changes (two studies), mean difference: 0.31 (95%CI -0.17, 0.79) kg/m2 were found. Neither in MNA scores, muscle strength, activities of daily living, timed Up&Go, quality of life and mortality. Results of other intervention studies (dietary counselling and ONS, ONS combined with exercise, nutrition delivery systems) were inconsistent. The overall quality of the evidence was very low due to risk of bias and small sample size. CONCLUSIONS: This review has highlighted the lack of high quality evidence to indicate which interventions are effective in treating malnutrition in older people. High quality research studies are urgently needed in this area.


Assuntos
Suplementos Nutricionais , Desnutrição/dietoterapia , Atividades Cotidianas , Idoso , Peso Corporal , Exercício/fisiologia , Humanos , Força Muscular , Estado Nutricional , Qualidade de Vida
15.
Aging Clin Exp Res ; 31(7): 969-975, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-30276631

RESUMO

BACKGROUND: Polypharmacy and fall-risk increasing drugs (FRIDS) have been associated with injurious falls. We aimed to estimate the prevalence of polypharmacy and FRIDS in older patients discharged from an Orthogeriatric Unit after a hip fracture surgery. METHODS: This study describes the baseline findings of a 2-year retrospective cohort study. We included patients older than 80 years discharged from an Orthogeriatric Unit who were able to walk before surgery. Patient's baseline variables, total number of drugs, and FRIDS at hospital discharge were collected. RESULTS: We included 228 patients. The mean number of drugs and FRIDS prescribed at discharge was 11.6 ± 3.0 and 2.9 ± 1.6, respectively. Polypharmacy was prevalent in all patients except in three: 23.3% (5-9 drugs) and 75.9% (≥ 10 drugs). Only 11 patients had no FRIDS and 35.5% were on > 3 FRIDS. The most prevalent FRIDS were: agents acting on the renin-angiotensin system (43.9%) and anxiolytics (39.9%). The number of FRIDS was higher in patients with extreme polypharmacy (3.4 ± 1.5) than in those on 5-9 drugs (1.5 ± 1.0, p < 0.05). Independent people in performing instrumental activities had lower risk of extreme polypharmacy (≥ 10 drugs) or > 3 FRIDS: OR 0.39 (95% CI 0.18-0.83) and OR 0.41 (95% CI 0.20-0.84), respectively. People living in a nursing home had higher risk of > 3 FRIDS: OR 4.03 (95% CI 1.12-14.53). CONCLUSIONS: Polypharmacy and fall-risk increasing drugs are prevalent in patients discharged from orthogeriatric care after surgery for a hip fracture. Interventions on drug use at hospital discharge could have a potential impact on falls in this high-risk population.


Assuntos
Acidentes por Quedas/prevenção & controle , Efeitos Colaterais e Reações Adversas Relacionados a Medicamentos/epidemiologia , Polimedicação , Atividades Cotidianas , Idoso , Idoso de 80 Anos ou mais , Inibidores da Enzima Conversora de Angiotensina/efeitos adversos , Ansiolíticos/efeitos adversos , Estudos de Coortes , Feminino , Fraturas do Quadril/cirurgia , Humanos , Masculino , Alta do Paciente/estatística & dados numéricos , Padrões de Prática Médica/estatística & dados numéricos , Prevalência , Estudos Retrospectivos , Fatores de Risco
16.
Inflammopharmacology ; 27(2): 397-408, 2019 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-29372359

RESUMO

BACKGROUND: Annona crassiflora Mart., popularly known as "Araticum", is a native tree of the Brazilian Cerrado used in folk medicine for treatment of pain and inflammatory diseases. We proposed to analyze analgesic and anti-inflammatory properties of the filtrate (F1) and the precipitate (F2) of the hydroalcoholic fraction from the leaves of Annona crassiflora Mart. in mice. MATERIALS AND METHODS: Swiss mice were submitted to formalin-induced nociception test and tail-flick reflex test, to assess antinociceptive properties, and to the rota-rod test, for motor performance analyses. To evaluate anti-inflammatory properties, F1 and F2 were orally administered 1 h prior to the intrathoracic injection of carrageenan, zymosan, LPS, CXCL8, or vehicle in Balb/c mice and neutrophil infiltration was evaluated 4 h after injection. RESULTS: F1 and F2 reduced the licking time in the second phase of formalin-induced nociception test, but only F2 showed a dose-dependent response. Neither F1 nor F2 reduced the latency time in the tail-flick reflex test. In addition, motor performance alteration was not observed in F1- or F2-treated mice. F2 treatment significantly inhibited the neutrophilia induced by carrageenan, LPS, or CXCL8, but not zymosan. CONCLUSIONS: The experimental data demonstrated that hydroalcoholic fractions of Annona crassiflora Mart. leaves have remarkable anti-inflammatory and antinociceptive activities.


Assuntos
Analgésicos/farmacologia , Annona/química , Anti-Inflamatórios/farmacologia , Medição da Dor/efeitos dos fármacos , Extratos Vegetais/farmacologia , Folhas de Planta/química , Animais , Carragenina/farmacologia , Modelos Animais de Doenças , Camundongos , Camundongos Endogâmicos BALB C
18.
J Cell Physiol ; 234(1): 108-121, 2018 01.
Artigo em Inglês | MEDLINE | ID: mdl-30076704

RESUMO

More than 40% of patients with luminal breast cancer treated with endocrine therapy agent tamoxifen demonstrate resistance. Emerging evidence suggests tumor initiating cells (TICs) and aberrant activation of Src and Akt signaling drive tamoxifen resistance and relapse. We previously demonstrated that aryl hydrocarbon receptor ligand aminoflavone (AF) inhibits the expression of TIC gene α6-integrin and disrupts mammospheres derived from tamoxifen-sensitive breast cancer cells. In the current study, we hypothesize that tamoxifen-resistant (TamR) cells exhibit higher levels of α6-integrin than tamoxifen-sensitive cells and that AF inhibits the growth of TamR cells by suppressing α6-integrin-Src-Akt signaling. In support of our hypothesis, TamR cells and associated mammospheres were found to exhibit elevated α6-integrin expression compared with their tamoxifen-sensitive counterparts. Furthermore, tumor sections from patients who relapsed on tamoxifen showed enhanced α6-integrin expression. Gene expression profiling from the TCGA database further revealed that basal-like breast cancer samples, known to be largely unresponsive to tamoxifen, demonstrated higher α6-integrin levels than luminal breast cancer samples. Importantly, AF reduced TamR cell viability and disrupted TamR mammospheres while concomitantly reducing α6-integrin messenger RNA and protein levels. In addition, AF and small interfering RNA against α6-integrin blocked tamoxifen-stimulated proliferation of TamR MCF-7 cells and further sensitized these cells to tamoxifen. Moreover, AF reduced Src and Akt signaling activation in TamR MCF-7 cells. Our findings suggest elevated α6-integrin expression is associated with tamoxifen resistance and AF suppresses α6-integrin-Src-Akt signaling activation to confer activity against TamR breast cancer.


Assuntos
Neoplasias da Mama/tratamento farmacológico , Flavonoides/farmacologia , Integrina alfa6/genética , Receptores de Hidrocarboneto Arílico/genética , Neoplasias da Mama/genética , Neoplasias da Mama/patologia , Resistencia a Medicamentos Antineoplásicos/genética , Feminino , Perfilação da Expressão Gênica , Regulação Neoplásica da Expressão Gênica , Humanos , Ligantes , Células MCF-7 , Células-Tronco Neoplásicas/efeitos dos fármacos , Proteína Oncogênica v-akt/genética , Receptores de Hidrocarboneto Arílico/antagonistas & inibidores , Transdução de Sinais , Tamoxifeno/efeitos adversos , Quinases da Família src/genética
20.
J Cell Physiol ; 233(6): 4677-4687, 2018 06.
Artigo em Inglês | MEDLINE | ID: mdl-29111571

RESUMO

Breast cancer is the first cause of cancer death in women. Many patients are resistant to current therapies, and even those were sensitive at first may eventually become resistant later. Thiosemicarbazones (TSCs) are synthetic compounds that exhibit several pharmacological activities. In this study, we investigated the potential anti-tumor activity of a set of N4 -arylsubstituted TSCs (N4 -TSCs) on human breast cancer cell lines. Studies on the effect of N4 -TSCs (T1, T2, and T3) were carried on MCF-7, MDA-MB 231, and BT 474 cell lines which differ in their expression of ER, PR, and Her2/neu. Non-transformed MCF-10A breast cell line were used as normal cells. Action of N4 -TSCs were evaluated by proliferation assay, quantification of apoptosis and cell cycle analysis. Modulation of clonogenic efficiency and migratory capacity by N4 -TSCs were also evaluated. We further investigated the effects of N4 -TSCs on ROS level and Ribonucleotide Reductase (RR) activity. We analyzed the action of these compounds on cellular mammosphere-forming capacity. We found that T1 and T2 had specific anti-tumor effect on all breast cancer cell lines based on their pro-apoptotic action and inhibitory effect on clonogenic efficiency and cell migration capacity. We also showed that both compounds increased ROS level and inhibited RR activity. Finally, we found that all N4 -TSCs diminished mammospehere-forming capacity of MCF-7 and BT 474 cells. N4 -TSCs showed specific anti-tumor action on human breast cancer cells independently their biomarkers expression pattern. Our results place these compounds as promising novel anti-tumor drugs with potential therapeutic application against different types of breast cancer.


Assuntos
Antineoplásicos/farmacologia , Neoplasias da Mama/tratamento farmacológico , Indanos/farmacologia , Tiossemicarbazonas/farmacologia , Antineoplásicos/síntese química , Apoptose/efeitos dos fármacos , Biomarcadores Tumorais/metabolismo , Neoplasias da Mama/metabolismo , Neoplasias da Mama/patologia , Movimento Celular/efeitos dos fármacos , Proliferação de Células/efeitos dos fármacos , Relação Dose-Resposta a Droga , Feminino , Humanos , Indanos/síntese química , Células MCF-7 , Necrose , Espécies Reativas de Oxigênio/metabolismo , Ribonucleotídeo Redutases/antagonistas & inibidores , Ribonucleotídeo Redutases/metabolismo , Transdução de Sinais/efeitos dos fármacos , Tiossemicarbazonas/síntese química
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA