Your browser doesn't support javascript.
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 47
Filtrar
1.
J Virol Methods ; 259: 54-59, 2018 09.
Artigo em Inglês | MEDLINE | ID: mdl-29902492

RESUMO

A new molecular assay (Viral CNS Flow Chip kit, Master Diagnóstica, Spain) has been developed for the detection of eight viruses causing acute meningitis and encephalitis, i.e. herpes simplex viruses 1-2, varicella zoster virus, human enterovirus, human parechovirus, Toscana virus, human cytomegalovirus and Epstein Barr virus. The new assay is a multiplex one-step RT-PCR followed by automatic flow-through hybridization, colorimetric detection and image analysis. The limit of detection was 50 copies/reaction, and 10 copies/reaction for human enterovirus and the other seven viruses, respectively. The analytical validation was performed with nucleic acids extracted from 268 cerebrospinal fluid samples and the results were compared with routine molecular assays. An excellent coefficient of agreement was observed between V-CNS and routine assays [kappa index: 0.948 (95%CI: 0.928-0.968)]. The overall sensitivity and specificity was 95.9% (95%CI: 91.2-98.3%) and 99.9% (95%CI: 99.6-100%), respectively. Viral CNS Flow Chip kit is an efficient multiplex platform for the detection of the main viruses involved in acute meningitis and encephalitis. The inclusion of a TOSV genome target may improve the laboratory diagnosis of viral neurological infections in endemic areas.


Assuntos
Encefalite/diagnóstico , Meningite/diagnóstico , Técnicas de Diagnóstico Molecular/métodos , Reação em Cadeia da Polimerase Multiplex/métodos , Técnicas de Amplificação de Ácido Nucleico , Reação em Cadeia da Polimerase em Tempo Real/métodos , Vírus/isolamento & purificação , Líquido Cefalorraquidiano/virologia , Colorimetria/métodos , Encefalite/virologia , Humanos , Processamento de Imagem Assistida por Computador/métodos , Meningite/virologia , Sensibilidade e Especificidade , Espanha , Vírus/classificação , Vírus/genética
2.
Euro Surveill ; 23(14)2018 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-29637890

RESUMO

In Andalusia, Spain, West Nile virus (WNV) surveillance takes place from April to November, during the active vector period. Within this area seroconversion to this virus was evidenced in wild birds in 2004, affecting horses and two humans for the first time in 2010. Since 2010, the virus has been isolated every year in horses, and national and regional surveillance plans have been updated with the epidemiological changes found. WNV is spreading rapidly throughout southern Europe and has caused outbreaks in humans. Here we describe the second WNV outbreak in humans in Andalusia, with three confirmed cases, which occurred between August and September 2016, and the measures carried out to control it. Surveillance during the transmission season is essential to monitor and ensure prompt identification of any outbreaks.

3.
Eur J Nutr ; 2017 Nov 09.
Artigo em Inglês | MEDLINE | ID: mdl-29124387

RESUMO

PURPOSE: Elderly people are particularly vulnerable to seasonal influenza. Therefore, vaccination is strongly recommended. However, the vaccine efficacy is lower in the elderly, owing to immunosenescence. The objective of the present study was to evaluate the ability of the probiotic strain Lactobacillus coryniformis K8 CECT5711 to enhance the immune response to the influenza vaccine in the elderly and to assess the effects on symptoms related to respiratory infections. METHODS: A randomized, double-blind, placebo-controlled trial was conducted between November 2015 and April 2016. A total of 98 nursing home residents, more than 65 years of age were randomly assigned to receive L. coryniformis K8 CECT5711 (3 × 109 CFU/day) or a placebo for 2 weeks before influenza vaccination. The primary outcome was the percentage of seroconversion. The secondary outcomes were the incidence of influenza-like illness (ILI) and respiratory symptoms associated with respiratory infections during the 5-month follow-up period. The serum cytokine and immunoglobulin levels were also evaluated. RESULTS: The percentage of responders to vaccination was higher in the probiotic group than in the control group (p = 0.036). L. coryniformis ingestion was associated with a significantly lower incidence of respiratory symptoms commonly associated with respiratory infections (p = 0.007) and lower consumption of analgesics (p = 0.008). CONCLUSION: The administration of L. coryniformis K8 CECT5711 to an elderly population increased the immune response against the influenza vaccine and decreased symptoms associated with respiratory infections. Probiotic administration may be a natural and safe strategy to improve the efficacy of vaccines and to protect against common respiratory infections in susceptible populations.

4.
PLoS Pathog ; 13(10): e1006650, 2017 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-29023600

RESUMO

Influenza A virus (IAV) infection can be severe or even lethal in toddlers, the elderly and patients with certain medical conditions. Infection of apparently healthy individuals nonetheless accounts for many severe disease cases and deaths, suggesting that viruses with increased pathogenicity co-circulate with pandemic or epidemic viruses. Looking for potential virulence factors, we have identified a polymerase PA D529N mutation detected in a fatal IAV case, whose introduction into two different recombinant virus backbones, led to reduced defective viral genomes (DVGs) production. This mutation conferred low induction of antiviral response in infected cells and increased pathogenesis in mice. To analyze the association between low DVGs production and pathogenesis in humans, we performed a genomic analysis of viruses isolated from a cohort of previously healthy individuals who suffered highly severe IAV infection requiring admission to Intensive Care Unit and patients with fatal outcome who additionally showed underlying medical conditions. These viruses were compared with those isolated from a cohort of mild IAV patients. Viruses with fewer DVGs accumulation were observed in patients with highly severe/fatal outcome than in those with mild disease, suggesting that low DVGs abundance constitutes a new virulence pathogenic marker in humans.


Assuntos
Genoma Viral/genética , Vírus da Influenza A Subtipo H1N1/genética , Virus da Influenza A Subtipo H5N1/genética , Influenza Humana/virologia , Infecções por Orthomyxoviridae/virologia , Replicação Viral/genética , Adolescente , Adulto , Animais , Criança , Pré-Escolar , Feminino , Humanos , Vírus da Influenza A Subtipo H1N1/patogenicidade , Virus da Influenza A Subtipo H5N1/patogenicidade , Masculino , Camundongos , Pessoa de Meia-Idade , Infecções por Orthomyxoviridae/genética , Virulência/genética , Adulto Jovem
5.
Enferm. infecc. microbiol. clín. (Ed. impr.) ; 35(7): 438-440, ago.-sept. 2017. tab
Artigo em Inglês | IBECS | ID: ibc-165241

RESUMO

The analytical performance of the new Alere(TM) i Influenza A&B kit (AL-Flu) assay, based on isothermal nucleic acids amplification, was evaluated and compared with an antigen detection method, SD Bioline Influenza Virus Antigen Test (SDB), and an automated real-time RT-PCR, Simplexa(TM) Flu A/B & VRS Direct assay (SPX), for detection of influenza viruses. An ‘in-house’ RT-PCR was used as the reference method. Sensitivity of AL-Flu, SDB, and SPX was 71.7%, 34.8%, and 100%, respectively. Specificity was 100% for all techniques. The turnaround time was 13min for AL-Flu, 15min for SDB, and 75min for SPX. The Alere(TM) i Influenza A&B assay is an optimal point-of-care assay for influenza diagnosis in clinical emergency settings, and is more sensitive and specific than antigen detection methods (AU)


Se evaluó el nuevo ensayo Alere(TM) i Influenza A&B kit (AL-Flu), basado en la amplificación isotérmica de ácidos nucleicos, y se comparó con un método de detección de antígeno, SD Bioline Influenza Virus Antigen Test (SDB), y con una RT-PCR en tiempo real automática, Simplexa(TM) Flu A/B & VRS Direct assay (SPX), para la detección de virus de la gripe. Se utilizó una RT-PCR en tiempo real casera como método de referencia. La sensibilidad de AL-Flu, SDB y SPX fue del 71,7%, del 34,8% y del 100%, respectivamente. Se obtuvo una especificidad del 100% con todos los métodos. El tiempo de realización fue de 13min para AL-Flu, de 15min para SDB y de 75min para SPX. El ensayo Alere(TM) i Influenza A&B es óptimo para el diagnóstico de gripe en unidades de urgencias, al ser más sensible y específico que las técnicas de detección de antígeno (AU)


Assuntos
Humanos , Influenzavirus A/isolamento & purificação , Influenzavirus B/isolamento & purificação , Influenza Humana/microbiologia , Infecções Respiratórias/microbiologia , Diagnóstico Precoce , Estudos Retrospectivos , Técnicas de Diagnóstico Molecular/métodos
6.
Enferm Infecc Microbiol Clin ; 35(7): 438-440, 2017 Aug - Sep.
Artigo em Inglês, Espanhol | MEDLINE | ID: mdl-26620605

RESUMO

The analytical performance of the new Alere™ i Influenza A&B kit (AL-Flu) assay, based on isothermal nucleic acids amplification, was evaluated and compared with an antigen detection method, SD Bioline Influenza Virus Antigen Test (SDB), and an automated real-time RT-PCR, Simplexa™ Flu A/B & VRS Direct assay (SPX), for detection of influenza viruses. An "in-house" RT-PCR was used as the reference method. Sensitivity of AL-Flu, SDB, and SPX was 71.7%, 34.8%, and 100%, respectively. Specificity was 100% for all techniques. The turnaround time was 13min for AL-Flu, 15min for SDB, and 75min for SPX. The Alere™ i Influenza A&B assay is an optimal point-of-care assay for influenza diagnosis in clinical emergency settings, and is more sensitive and specific than antigen detection methods.


Assuntos
Vírus da Influenza A/isolamento & purificação , Vírus da Influenza B/isolamento & purificação , Influenza Humana/diagnóstico , Influenza Humana/virologia , Humanos , Técnicas de Diagnóstico Molecular , Técnicas de Amplificação de Ácido Nucleico , Estudos Retrospectivos , Fatores de Tempo
7.
Rev. esp. quimioter ; 29(6): 332-335, dic. 2016. tab
Artigo em Inglês | IBECS | ID: ibc-158228

RESUMO

Introduction. Different subtypes of Campylobacter spp. have been associated with diarrhoea and a Multilocus Sequence Typing (MLST) method has been performed for subtyping. In the present work, MLST was used to analyse the genetic diversity of eight strains of Campylobacter coli. Material and methods. Nineteen genetic markers were amplified for MLST analysis: AnsB, DmsA, ggt, Cj1585c, CJJ81176-1367/1371, Tlp7, cj1321-cj1326, fucP, cj0178, cj0755/cfrA, ceuE, pldA, cstII, cstIII. After comparing the obtained sequences with the Campylobacter MLST database, the allele numbers, sequence types (STs) and clonal complexes (CCs) were assigned. Results. The 8 C. coli isolates yielded 4 different STs belonging to 2 CCs. Seven isolates belong to ST-828 clonal complex and only one isolate belong to ST-21. Two samples came from the same patient, but were isolated in two different periods of time. Conclusions. MLST can be useful for taxonomic characterization of C. coli isolates (AU)


Introducción. Diferentes subtipos de Campylobacter spp. se han asociado con diarrea y la técnica de tipado mediante análisis de secuencias de múltiples locus (MLST) se ha empleado para la tipificación genética. En el presente trabajo, la técnica MLST se utilizó para analizar la diversidad genética de ocho cepas de Campylobacter coli. Material y métodos. 19 marcadores genéticos fueron amplificados mediante el análisis MLST: AnsB, DmsA, ggt, Cj1585c, CJJ81176-1367/1371, Tlp7, cj1321-cj1326, fucP, cj0178, cj0755/cfrA, ceuE, pldA, cstII, cstIII. Después de comparar las secuencias obtenidas con la base de datos MLST para Campylobacter, se asignaron el número de los alelos, los secuenciotipos (STs) y los complejos clonales (CCs). Resultados. Las 8 cepas de C. coli aisladas mostraron 4 STs diferentes pertenecientes a 2 CCs. Siete aislamientos pertenecieron al complejo clonal ST-828 y sólo un aislado perteneció al ST-21. Dos aislados pertenecieron al mismo paciente, pero fueron obtenidos en diferentes periodos de tiempo. Conclusiones. La técnica MLST puede ser útil para la caracterización taxonómica de aislados de C. coli (AU)


Assuntos
Humanos , Masculino , Feminino , Tipagem de Sequências Multilocus/métodos , Tipagem de Sequências Multilocus , Campylobacter coli/genética , Campylobacter coli/isolamento & purificação , Marcadores Genéticos/genética , Marcadores Genéticos/fisiologia , Análise de Sequência de DNA/métodos , Tipagem de Sequências Multilocus/classificação , Tipagem de Sequências Multilocus/normas , Infecções por Campylobacter/classificação , Infecções por Campylobacter/diagnóstico , Infecções por Campylobacter/genética
8.
Diagn Microbiol Infect Dis ; 83(3): 252-6, 2015 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-26283523

RESUMO

The analytical performance of mariPOC® respi test (ArcDia® Laboratories, Turku, Finland) was evaluated using nucleic acid amplification techniques (NAATs) as the gold standard. The mariPOC assay allows automated detection of antigens from 8 respiratory viruses: influenza A and B viruses, respiratory syncytial virus, adenovirus, human metapneumovirus, and parainfluenza viruses 1-3. Positive results from samples with high viral load are available in 20min. Nasopharyngeal aspirates (n=192) from patients with acute respiratory infection and from previously positive samples were analyzed by mariPOC and NAATs (Simplexa(TM) FluA/FluB & RSV kit [n=118] and Luminex® Respiratory virus panel xTAG® RVP FAST [n=74]). Sensitivity, specificity, positive predictive value, and negative predictive value of mariPOC were 85.4%, 99.2%, 95.9%, and 97%, respectively, and 84.6% of positive results were reported in 20min. The good analytical performance and extended portfolio of mariPOC show this rapid assay as a good alternative for the etiological diagnosis of acute respiratory infection in laboratories that are not equipped with molecular assays.


Assuntos
Antígenos Virais/análise , Automação Laboratorial/métodos , Imunoensaio/métodos , Sistemas Automatizados de Assistência Junto ao Leito , Infecções Respiratórias/diagnóstico , Viroses/diagnóstico , Vírus/isolamento & purificação , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Criança , Feminino , Humanos , Recém-Nascido , Pessoa de Meia-Idade , Nasofaringe/virologia , Valor Preditivo dos Testes , Sensibilidade e Especificidade , Fatores de Tempo , Vírus/classificação , Adulto Jovem
9.
Eur J Pediatr ; 174(11): 1511-6, 2015 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-25982340

RESUMO

UNLABELLED: Human parechoviruses (HPeV) have been recently recognized as important viral agents in paediatric infections. The aims of this study were to investigate the HPeV infection prevalence in infants <1 month in Spain and, secondly, to analyse the clinical and epidemiological characteristics of the infected patients compared with those infected by enterovirus (EV). Infants <1 month with neurological or systemic symptoms were included in a multicentre prospective study. EV and HPeV detection by RT-PCR and genotyping were performed in cerebrospinal fluids (CSF), sera or throat swabs. Out of the total of 84 infants studied during 2013, 32 were EV positive (38 %) and 9 HPeV positive (11 %). HPeV-3 was identified in eight cases and HPeV-5 in one. Mean age of HPeV-positive patients was 18 days. Diagnoses were fever without source (FWS) (67 %), clinical sepsis (22 %) and encephalitis (11 %). Leukocytes in blood and CSF were normal. Pleocytosis (p = 0.03) and meningitis (p = 0.001) were significantly more frequent in patients with EV infections than with HPeV. CONCLUSIONS: Although HPeV-3 infections were detected less frequently than EV, they still account for approximately 10 % of the cases analysed in infants younger than 1 month. HPeV-3 was mainly associated with FWS and without leukocytosis and pleocytosis in CSF. In these cases, HPeV screening is desirable to identify the aetiologic agent and prevent unnecessary treatment and prolonged hospitalization.


Assuntos
Encefalite Viral/epidemiologia , Infecções por Enterovirus/epidemiologia , Enterovirus/isolamento & purificação , Parechovirus/isolamento & purificação , Infecções por Picornaviridae/epidemiologia , Viremia/epidemiologia , Encefalite Viral/diagnóstico , Encefalite Viral/virologia , Enterovirus/genética , Infecções por Enterovirus/diagnóstico , Infecções por Enterovirus/virologia , Feminino , Genótipo , Humanos , Recém-Nascido , Masculino , Parechovirus/genética , Infecções por Picornaviridae/diagnóstico , Infecções por Picornaviridae/virologia , Prevalência , Estudos Prospectivos , Reação em Cadeia da Polimerase em Tempo Real , Espanha/epidemiologia , Viremia/diagnóstico , Viremia/virologia
10.
J Virol Methods ; 208: 125-8, 2014 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-25152526

RESUMO

Human enteroviruses (EVs) and parechoviruses (HPeVs) are important etiological agents causing infections such as meningitis, encephalitis and sepsis-like disease in neonates and young children. We have developed a real-time RT-PCR for simultaneous detection of EV and HPeV in clinical samples. Primers and probe sets were designed from the conserved 5'-noncoding region of the genomes. The sensitivity, specificity and reproducibility of the technique were measured using a set of 25 EV and 6 HPeV types. All EVs but no HPeVs were detected with the EV primers-probe set. The HPeV primers-probe set detected only the 6 HPeV types. The lower detection limit was found to be 4 and 40CCID50/ml for HPeV and EV respectively, demonstrating high sensitivity of the technique for both viruses. The threshold cycle values were highly reproducible on repeat testing of positive controls among assay runs. The assay was evaluated in 53 clinical samples of suspected meningitis, sepsis or febrile syndromes from children under 3 years. In 11 of these (21%) EVs were detected, while 4, i.e. 7.5%, were HPeV positive. Molecular typing was carried out for 73% of the viruses. In summary, the RT-PCR method developed demonstrated effectively both EV and HPeV detection, which can cause similar clinical symptoms in infants.


Assuntos
Enterovirus/isolamento & purificação , Técnicas de Diagnóstico Molecular/métodos , Parechovirus/isolamento & purificação , Infecções por Picornaviridae/diagnóstico , Reação em Cadeia da Polimerase em Tempo Real/métodos , Reação em Cadeia da Polimerase Via Transcriptase Reversa/métodos , Pré-Escolar , Primers do DNA/genética , Enterovirus/classificação , Enterovirus/genética , Humanos , Lactente , Sondas de Oligonucleotídeos/genética , Parechovirus/classificação , Parechovirus/genética , Infecções por Picornaviridae/virologia , RNA não Traduzido/genética , RNA Viral/genética , Reprodutibilidade dos Testes , Sensibilidade e Especificidade
11.
Enferm. infecc. microbiol. clín. (Ed. impr.) ; 32(supl.1): 15-22, feb. 2014. ilus, tab
Artigo em Espanhol | IBECS | ID: ibc-134455

RESUMO

La infección por citomegalovirus humano (CMV) tiene una altísima prevalencia mundial. Tras la infección primaria, el virus pasa a un estado de latencia, pudiendo aparecer recurrencias por reinfección con una cepa nueva o por reactivación de la replicación del CMV latente. Los cuadros clínicos más graves se dan en infección congénita y en pacientes inmunodeprimidos, en los que se comporta como patógeno oportunista. Las técnicas serológicas son de elección en la infección primaria y para determinar el estado inmune frente a CMV en el donante y receptor de órganos. Aunque faltan estudios estandarizados, la reciente comercialización de métodos de medida de la respuesta inmune celular ofrece buenas perspectivas para predecir el riesgo de enfermedad por CMV en inmunodeprimidos. Las técnicas moleculares, que han ido sustituyendo al cultivo y/o detección de antígeno, son actualmente los procedimientos más utilizados en el diagnóstico de rutina y control de la infección por CMV (AU)


Prevalence of human cytomegalovirus infection is very high worldwide. Following primary infection, the virus remains latent, being able to cause recurrences either by reinfection with a new strain or by reactivation of the replication of the latent virus. The most severe disease is seen in congenital infection and in immunosuppressed patients, in whom the virus act as an opportunistic pathogen. Serological techniques are the methods of choice in primary infection and to determine the immune status against CMV in organ donor and receptor. Although well-standardized studies are lacking, the recent commercial availability of methods that measure cellular immune response are promising to predict the risk of CMV disease in immunosuppressed individuals. Molecular assays, that have gradually been substituting viral culture and/or antigen detection, are the most widely used methods for the diagnosis and control of CMV infection (AU)


Assuntos
Humanos , Infecções por Citomegalovirus/microbiologia , Citomegalovirus/isolamento & purificação , DNA Viral/análise , Testes Sorológicos/métodos , Antígenos de Plaquetas Humanas/análise , Cultura de Vírus/métodos , Reação em Cadeia da Polimerase/métodos
12.
Enferm. infecc. microbiol. clín. (Ed. impr.) ; 32(supl.1): 15-22, feb. 2014. tab, graf
Artigo em Espanhol | IBECS | ID: ibc-179629

RESUMO

La infección por citomegalovirus humano (CMV) tiene una altísima prevalencia mundial. Tras la infección primaria, el virus pasa a un estado de latencia, pudiendo aparecer recurrencias por reinfección con una cepa nueva o por reactivación de la replicación del CMV latente. Los cuadros clínicos más graves se dan en infección congénita y en pacientes inmunodeprimidos, en los que se comporta como patógeno oportunista. Las técnicas serológicas son de elección en la infección primaria y para determinar el estado inmune frente a CMV en el donante y receptor de órganos. Aunque faltan estudios estandarizados, la reciente comercialización de métodos de medida de la respuesta inmune celular ofrece buenas perspectivas para predecir el riesgo de enfermedad por CMV en inmunodeprimidos. Las técnicas moleculares, que han ido sustituyendo al cultivo y/o detección de antígeno, son actualmente los procedimientos más utilizados en el diagnóstico de rutina y control de la infección por CMV


Prevalence of human cytomegalovirus infection is very high worldwide. Following primary infection, the virus remains latent, being able to cause recurrences either by reinfection with a new strain or by reactivation of the replication of the latent virus. The most severe disease is seen in congenital infection and in immunosuppressed patients, in whom the virus act as an opportunistic pathogen. Serological techniques are the methods of choice in primary infection and to determine the immune status against CMV in organ donor and receptor. Although well-standardized studies are lacking, the recent commercial availability of methods that measure cellular immune response are promising to predict the risk of CMV disease in immunosuppressed individuals. Molecular assays, that have gradually been substituting viral culture and/or antigen detection, are the most widely used methods for the diagnosis and control of CMV infection


Assuntos
Humanos , Infecções por Citomegalovirus/diagnóstico , Infecções por Citomegalovirus/virologia , Citomegalovirus , Citomegalovirus/isolamento & purificação
13.
Enferm. infecc. microbiol. clín. (Ed. impr.) ; 31(6): 375-379, jun.-jul. 2013. tab
Artigo em Espanhol | IBECS | ID: ibc-114561

RESUMO

Introducción La sepsis neonatal es una importante causa de morbimortalidad. Un diagnóstico precoz y el inicio rápido del tratamiento son fundamentales en la evolución del recién nacido. El hemocultivo, considerado técnica de referencia para el diagnóstico de sepsis, presenta una baja sensibilidad en este grupo de pacientes. Se planteó evaluar la utilidad de la PCR múltiple LightCycler® SeptiFast (LC-SF) en el diagnóstico de la sepsis neonatal, comparándola con el hemocultivo tradicional. Métodos Se recogieron 42 muestras de sangre correspondientes a 35 recién nacidos con episodios febriles ingresados en la unidad de cuidados intensivos neonatal del Hospital Universitario Virgen de las Nieves. Por cada episodio febril se procesaron 2 muestras de sangre venosa periférica para la realización del ensayo LC-SF y del hemocultivo, respectivamente. Resultados La sensibilidad y la especificidad de LC-SF, comparada con el diagnóstico clínico de sepsis, fueron del 79 y del 87%, respectivamente. La tasa de hemocultivos contaminados fue del 16,7%, detectándose Staphylococcus coagulasa negativa (SCN) y Streptococcus grupo viridans. En LC-SF la tasa de SCN contaminantes fue del 2,4%. La concordancia entre las 2 técnicas diagnósticas fue moderada (índice kappa: 0,369). La técnica LC-SF demostró una mayor concordancia con el diagnóstico clínico final (índice kappa: 0,729) que el hemocultivo (índice kappa: 0,238). Conclusión LC-SF podría ser una herramienta útil, empleada en paralelo con el hemocultivo en recién nacidos, al confirmar o descartar los casos que el hemocultivo no resuelve, además de disminuir el tiempo de obtención de resultado a 7 h( AU)


Introduction Neonatal sepsis is a significant cause of morbidity and mortality. Early diagnosis and prompt antimicrobial therapy are crucial for a favorable outcome of the newborn child. Blood culture, the current “gold standard” method for diagnosing bloodstream infections, has a low sensitivity in newborns. We evaluated the multiplex real-time PCR LightCycler® SeptiFast (LC-SF) for detection of bloodstream infections in newborns, compared with conventional blood culture. Methods A total of 42 blood samples were obtained from 35 subjects presenting with a febrile episode and hospitalized in neonatal intensive care unit at Hospital Universitario Virgen de las Nieves. Two samples were collected during each febrile episode in order to carry out LC-SF assay and blood culture, respectively. Results Sensitivity and specificity of 79% and 87%, respectively, compared with clinical diagnosis, were obtained for LC-SF. Contamination rate of blood cultures was 16.7%, mainly due to coagulase-negative staphylococci (CoNS) and viridans groups of streptococci. Contamination rate of LC-SF by CoNS was 2.4%. Concordance between LC-SF and blood culture was moderate (kappa index: 0.369). LC-SF demonstrated a higher concordance (kappa index: 0.729) with the final clinical diagnosis than blood culture (kappa index: 0.238). Conclusion LC-SF assay could be a useful diagnostic tool, along with a conventional blood culture, in newborn, for confirming or ruling out those cases that blood culture could not determine, shortening the time to result to 7 hours (AU)


Assuntos
Humanos , Masculino , Feminino , Recém-Nascido , Lactente , Sepse/microbiologia , Técnicas Microbiológicas/métodos , Reação em Cadeia da Polimerase/métodos , Doenças do Recém-Nascido/microbiologia , Fatores de Risco
14.
Am J Trop Med Hyg ; 88(5): 1003-6, 2013 May.
Artigo em Inglês | MEDLINE | ID: mdl-23419365

RESUMO

Granada virus (GRV), a new phlebovirus within the Naples serocomplex, has been recently described in phlebotomine sandflies from Spain. The presence of anti-GRV immunoglobulin G (IgG) antibodies was investigated by indirect fluorescence assay (IFA) and neutralization test (NT) in 920 serum samples from the Granada population. By IFA, an overall GRV seroprevalence of 15.8% (N = 145) was observed, significantly increasing up to 65 years. NT was positive in 18% of anti-GRV IFA-positive samples. IgG antibodies against Toscana virus (TOSV), a hyperendemic phlebovirus within Granada province, were detected in 40% of anti-GRV-positive cases. Anti-GRV IgM antibodies were detected in 36 (6.6%) of 547 acute-phase serum samples from individuals with febrile illness, exanthema, and/or acute respiratory infection. All positives were anti-TOSV IgM-negative. GRV may infect humans, with most cases being asymptomatic. The codetection of anti-GRV and anti-TOSV IgG antibodies could be attributable to cross-reactivity or exposure to the same transmission vector.


Assuntos
Anticorpos Antivirais/sangue , Febre por Flebótomos/epidemiologia , Febre por Flebótomos/fisiopatologia , Phlebovirus/imunologia , Adulto , Idoso , Animais , Reações Cruzadas , Humanos , Imunoglobulina G/sangue , Imunoglobulina M/sangue , Pessoa de Meia-Idade , Febre por Flebótomos/virologia , Psychodidae/virologia , Vírus da Febre do Flebótomo Napolitano/imunologia , Estudos Soroepidemiológicos , Espanha/epidemiologia
15.
Enferm Infecc Microbiol Clin ; 31(6): 375-9, 2013 Jun-Jul.
Artigo em Espanhol | MEDLINE | ID: mdl-23137657

RESUMO

INTRODUCTION: Neonatal sepsis is a significant cause of morbidity and mortality. Early diagnosis and prompt antimicrobial therapy are crucial for a favorable outcome of the newborn child. Blood culture, the current "gold standard" method for diagnosing bloodstream infections, has a low sensitivity in newborns. We evaluated the multiplex real-time PCR LightCycler(®) SeptiFast (LC-SF) for detection of bloodstream infections in newborns, compared with conventional blood culture. METHODS: A total of 42 blood samples were obtained from 35 subjects presenting with a febrile episode and hospitalized in neonatal intensive care unit at Hospital Universitario Virgen de las Nieves. Two samples were collected during each febrile episode in order to carry out LC-SF assay and blood culture, respectively. RESULTS: Sensitivity and specificity of 79% and 87%, respectively, compared with clinical diagnosis, were obtained for LC-SF. Contamination rate of blood cultures was 16.7%, mainly due to coagulase-negative staphylococci (CoNS) and viridans groups of streptococci. Contamination rate of LC-SF by CoNS was 2.4%. Concordance between LC-SF and blood culture was moderate (kappa index: 0.369). LC-SF demonstrated a higher concordance (kappa index: 0.729) with the final clinical diagnosis than blood culture (kappa index: 0.238). CONCLUSION: LC-SF assay could be a useful diagnostic tool, along with a conventional blood culture, in newborn, for confirming or ruling out those cases that blood culture could not determine, shortening the time to result to 7 hours.


Assuntos
Reação em Cadeia da Polimerase em Tempo Real , Sepse/sangue , Sepse/diagnóstico , Técnicas Bacteriológicas , Feminino , Humanos , Lactente , Recém-Nascido , Masculino , Estudos Retrospectivos
16.
Open Virol J ; 6: 151-9, 2012.
Artigo em Inglês | MEDLINE | ID: mdl-23248735

RESUMO

Advances in clinical virology for detecting respiratory viruses have been focused on nucleic acids amplification techniques, which have converted in the reference method for the diagnosis of acute respiratory infections of viral aetiology. Improvements of current commercial molecular assays to reduce hands-on-time rely on two strategies, a stepwise automation (semi-automation) and the complete automation of the whole procedure. Contributions to the former strategy have been the use of automated nucleic acids extractors, multiplex PCR, real-time PCR and/or DNA arrays for detection of amplicons. Commercial fully-automated molecular systems are now available for the detection of respiratory viruses. Some of them could convert in point-of-care methods substituting antigen tests for detection of respiratory syncytial virus and influenza A and B viruses. This article describes laboratory methods for detection of respiratory viruses. A cost-effective and rational diagnostic algorithm is proposed, considering technical aspects of the available assays, infrastructure possibilities of each laboratory and clinic-epidemiologic factors of the infection.

17.
Enferm Infecc Microbiol Clin ; 30 Suppl 4: 25-31, 2012 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-23116789

RESUMO

Coinciding with the pandemic wave of the influenza A(H1N1)pdm09 virus, other respiratory viruses have co-circulated in our area and were responsible for many acute respiratory infections and influenza-like illness (ILI). Apart from the pandemic virus that was responsible for most ILI cases, incidence rates of other viruses have varied among geographical areas. In general, human rhinovirus was the most frequent among individuals from the community, and respiratory syncytial virus among hospitalized patients. Detection rates of other respiratory viruses such as human metapneumovirus, adenovirus or parainfluenza viruses have been much lower. On the basis of an interference mechanism, human rhinovirus may contribute to modulate the pandemic wave, although available data are not conclusive to support this hypothesis. In contrast, the epidemic wave of respiratory syncytial virus during 2009-2010 was similar to previous seasons. Overall, incidence rates of respiratory viruses other than influenza did not change significantly during the pandemic season compared to other seasons. No association has been found between coinfection of pandemic influenza and other respiratory viruses with the prognosis of patients with influenza. The involvement of clinical virology laboratories in the etiological diagnosis of ILI cases has improved and has optimized diagnostic procedures.


Assuntos
Coinfecção , Influenza Humana/complicações , Influenza Humana/epidemiologia , Pandemias , Infecções Respiratórias/complicações , Infecções Respiratórias/virologia , Viroses/complicações , Algoritmos , Humanos , Estações do Ano
18.
Enferm. infecc. microbiol. clín. (Ed. impr.) ; 30(supl.4): 25-31, oct. 2012. ilus
Artigo em Inglês | IBECS | ID: ibc-105894

RESUMO

Coinciding with the pandemic wave of the influenza A(H1N1)pdm09 virus, other respiratory viruses have co-circulated in our area and were responsible for many acute respiratory infections and influenza-like illness (ILI). Apart from the pandemic virus that was responsible for most ILI cases, incidence rates of other viruses have varied among geographical areas. In general, human rhinovirus was the most frequent among individuals from the community, and respiratory syncytial virus among hospitalized patients. Detection rates of other respiratory viruses such as human metapneumovirus, adenovirus or parainfluenza viruses have been much lower. On the basis of an interference mechanism, human rhinovirus may contribute to modulate the pandemic wave, although available data are not conclusive to support this hypothesis. In contrast, the epidemic wave of respiratory syncytial virus during 2009–2010 was similar to previous seasons. Overall, incidence rates of respiratory viruses other than influenza did not change significantly during the pandemic season compared to other seasons. No association has been found between coinfection of pandemic influenza and other respiratory viruses with the prognosis of patients with influenza. The involvement of clinical virology laboratories in the etiological diagnosis of ILI cases has improved and has optimized diagnostic procedures (AU)


Coincidiendo con la onda pandémica 2009 por el virus de la gripe A(H1N1)pdm09, otros virus respiratorios han circulado en nuestro medio, provocando numerosos casos de infección respiratoria aguda y de síndrome gripal (ILI, influenza-like illness). Aparte del virus pandémico, que fue responsable de la mayoría de los casos de ILI, la incidencia de otros virus ha sido diferente según la zona. En general, rinovirus fue el virus más frecuente en la comunidad y virus respiratorio sincitial en pacientes hospitalizados. Las tasas de detección de otros virus como metaneumovirus humano, adenovirus o virus parainfluenza han sido mucho menores. Sobre la base de un mecanismo de interferencia, la presencia de rinovirus pudo contribuir a modular la onda pandémica de gripe, aunque los datos existentes no apoyan esta hipótesis de modo concluyente, mientras que la onda de virus respiratorio sincitial en 2009-2010 se ha presentado de forma similar a otros años. En conjunto, la incidencia de los distintos virus respiratorios de gripe no varió significativamente durante la temporada de la pandemia con respecto a otros años. Por otro lado, no se ha asociado la coinfección por virus de la gripe con otros virus respiratorios con el pronóstico de los pacientes con gripe. La implicación de los laboratorios de virología clínica en el diagnóstico de ILI ha supuesto una mejora y una mayor optimización en los procedimientos diagnósticos (AU)


Assuntos
Humanos , Influenza Humana/epidemiologia , Vírus da Influenza A Subtipo H1N1/patogenicidade , Infecções Respiratórias/virologia , Coinfecção/epidemiologia , Pandemias
20.
Clin Biochem ; 45(4-5): 374-7, 2012 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-22240066

RESUMO

OBJECTIVES: To evaluate the implementation of a quality management system based on ISO-15189 in a urine culture unit. DESIGN AND METHODS: The effectiveness of improvement actions was measured by quality indicators. RESULTS: The errors in the pre-analytical phase and the rate of contaminated urine decreased significantly. The traceability, response time and external quality control were fulfilled. CONCLUSIONS: The implementation of ISO-15189 was effective in improving the management of a urine culture unit.


Assuntos
Implementação de Plano de Saúde , Garantia da Qualidade dos Cuidados de Saúde , Urina/microbiologia , Unidade Hospitalar de Urologia , Ilhas Atlânticas , Bactérias Gram-Negativas/classificação , Bactérias Gram-Negativas/efeitos dos fármacos , Bactérias Gram-Negativas/crescimento & desenvolvimento , Bactérias Gram-Negativas/isolamento & purificação , Bactérias Gram-Positivas/classificação , Bactérias Gram-Positivas/efeitos dos fármacos , Bactérias Gram-Positivas/crescimento & desenvolvimento , Bactérias Gram-Positivas/isolamento & purificação , Hospitais Universitários , Humanos , Testes de Sensibilidade Microbiana , Estudos de Casos Organizacionais , Melhoria de Qualidade , Indicadores de Qualidade em Assistência à Saúde , Estudos Retrospectivos , Espanha , Unidade Hospitalar de Urologia/normas
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA