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1.
Braz. j. biol ; 82: e238763, 2022. tab, graf
Artigo em Inglês | LILACS-Express | LILACS | ID: biblio-1180734

RESUMO

Abstract We report the discovery that the earwig predator Doru luteipes (Scudder, 1876) (Dermaptera: Forficulidae) feed on Puccinia polysora Underw uredospore, the causal agent of Southern Rust of Corn (SRC), which is a primary disease affecting the maize crop in Brazil. We performed experiments in laboratory and greenhouse to test the effect of D. luteipes (1st/2nd and 3rd/4th instars, and adults) fungivory on the P. polysora uredospore concentration. All trials showed a significant reduction of the initial concentration of uredospore. There was a reduction in uredospore concentration with increase in number of D. luteipes feeding on them. We also tested the uredospore consumption by quantifying its percentage in the feces of D. luteipes. Nymphs of the 2nd, 4th instar and adults fed 88%, 85%, and 83.8% of the uredospore, respectively. For nymphs of the 3rd instar, the percentage of uredospore consumption (75.6%) was statistically significant compared with the other groups. In greenhouse experiment, at twenty-eight days after plant inoculation with 9.9 x 104 uredospores, the percentage of uredospore consumption was 81.7%. Our results confirmed the fungivory of D. luteipes on P. polysora uredospore. This is the first report of D. luteipes fungivory, which may play an important role in the biological control of P. polysora in corn.


Resumo Relatamos a descoberta de que o predador Doru luteipes (Scudder, 1876) (Dermaptera: Forficulidae) se alimenta de uredosporos de Puccinia polysora Underw, o agente causal da ferrugem polisora que é uma doença primária que afeta a cultura do milho no Brasil. Realizamos experimentos em laboratório e em casa de vegetação para testar o efeito da fungivoria de D. luteipes (1º/2º e 3º/4º iìstares e adultos) sobre a concentração de uredosporos de P. polysora. Todos os ensaios mostraram uma redução significativa da concentração inicial de uredosporos. Houve uma redução na concentração de uredosporos com o aumento do número de D. luteipes alimentando-se deles. Também testamos o consumo de uredosporos quantificando sua porcentagem nas fezes de D. luteipes. Ninfas do 2º e 4º ínstar, assim como adultos, alimentaram-se de 88%, 85% e 83,8% dos uredosporos, respectivamente. Para ninfas do 3º ínstar, a porcentagem de consumo de uredosporos (75,6%) foi estatisticamente significativo em comparação com os outros grupos. No experimento em casa de vegetação, aos 28 dias após a inoculação das plantas com 9,9 x 104 uredosporos, a porcentagem de consumo de uredosporos foi de 81,7%. Nossos resultados confirmaram a fungivoria de D. luteipes em uredosporos de P. polysora. Este é o primeiro relato de fungivoria de D. luteipes, que pode ter papel importante no controle biológico de P. polysora em milho.

2.
Braz J Biol ; 82: e238763, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33825760

RESUMO

We report the discovery that the earwig predator Doru luteipes (Scudder, 1876) (Dermaptera: Forficulidae) feed on Puccinia polysora Underw uredospore, the causal agent of Southern Rust of Corn (SRC), which is a primary disease affecting the maize crop in Brazil. We performed experiments in laboratory and greenhouse to test the effect of D. luteipes (1st/2nd and 3rd/4th instars, and adults) fungivory on the P. polysora uredospore concentration. All trials showed a significant reduction of the initial concentration of uredospore. There was a reduction in uredospore concentration with increase in number of D. luteipes feeding on them. We also tested the uredospore consumption by quantifying its percentage in the feces of D. luteipes. Nymphs of the 2nd, 4th instar and adults fed 88%, 85%, and 83.8% of the uredospore, respectively. For nymphs of the 3rd instar, the percentage of uredospore consumption (75.6%) was statistically significant compared with the other groups. In greenhouse experiment, at twenty-eight days after plant inoculation with 9.9 x 104 uredospores, the percentage of uredospore consumption was 81.7%. Our results confirmed the fungivory of D. luteipes on P. polysora uredospore. This is the first report of D. luteipes fungivory, which may play an important role in the biological control of P. polysora in corn.


Assuntos
Zea mays , Animais , Brasil , Ninfa
3.
Braz. j. biol ; 80(4): 798-802, Oct.-Dec. 2020. tab, graf
Artigo em Inglês | LILACS-Express | LILACS | ID: biblio-1142516

RESUMO

Abstract Nasal mites are commonly found in hummingbirds (Apodiformes). In most cases, endoparasitic mites were reported to parasitize the respiratory system, particularly the anterior nasal chambers, larynx, trachea, lungs, and the air and conjunctival sacs. However, some mites are only carried by hummingbirds, because flowers serving as a nutrient source for these small mites, such as for Apodiformes, a tropical nectar-feeding in their natural habitat. From August 2012 to May 2014, humminbirds were captured using mist nets, and examined for the presence of nasal mites (identified according to specific keys) in the Pantanal, and Cerrado biomes, from the Mato Grosso state, midwestern Brazil. Overall, 76 Apodiform specimens, from the Trochilidae family were captured, and 20 female nasal mites, identified as being from the Rhinoseius genus, were collected from eight hosts (10.5%), of the following species: Amazilia fimbriata, Amazilia versicolor, Eupetonema macroura, and Thalurania furcata. The Rhinoseius spp. has been reported on, in many ecosystems in the Americas, including Brazil, however, this is the first report on Apodiformes from the Pantanal biome. Phoretic mites are not responsible for direct damage to the population of Apodiformes, as they are not pathogenic. However, they compete for food with hummingbirds and decrease the quantity of food available in the environment. They also affect the reproductive dynamics of plants, feed on pollen, and interfere with the intricacies of the biodiversity where they live.


Resumo Os ácaros nasais são comumente encontrados em beija-flores (Apodiformes). Na maioria dos casos, os ácaros foram relatados como endoparasitas, parasitando o sistema respiratório, particularmente as câmaras nasais anteriores, a laringe, a traqueia, os pulmões e os sacos aéreos e conjuntivais. No entanto, alguns ácaros são transportados apenas por beija-flores, pois flores servem como fonte de nutrientes para esses pequenos ácaros, assim como para Apodiformes tropicais alimentados de néctar em seu habitat natural. De agosto de 2012 a maio de 2014, beija-flores foram capturadas com redes de neblina e examinadas quanto à presença de ácaros nasais (identificados de acordo com chaves específicas) nos biomas Pantanal e Cerrado, no estado do Mato Grosso, Centro-Oeste do Brasil. No total, 76 exemplares Apodiformes da família Trochilidae foram capturados e 20 fêmeas de ácaros nasais, identificados como pertencentes ao gênero Rhinoseius, foram coletados de oito hospedeiros (10,5%), das seguintes espécies: Amazilia fimbriata, Amazilia versicolor, Eupetonema macroura e Thalurania furcata. Rhinoseius spp. foi relatado em muitos ecossistemas nas Américas, incluindo o Brasil, no entanto, este é o primeiro relato em Apodiformes do bioma Pantanal. Os ácaros foréticos não são responsáveis ​​por danos diretos à população de Apodiformes, pois não são patogênicos. No entanto, eles competem por comida com beija-flores e diminuem a quantidade de comida disponível no ambiente. Eles também afetam a dinâmica reprodutiva das plantas, se alimentam de pólen e interferem nas interações da biodiversidade em que vivem.

4.
Braz J Biol ; 80(4): 798-802, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-31826078

RESUMO

Nasal mites are commonly found in hummingbirds (Apodiformes). In most cases, endoparasitic mites were reported to parasitize the respiratory system, particularly the anterior nasal chambers, larynx, trachea, lungs, and the air and conjunctival sacs. However, some mites are only carried by hummingbirds, because flowers serving as a nutrient source for these small mites, such as for Apodiformes, a tropical nectar-feeding in their natural habitat. From August 2012 to May 2014, humminbirds were captured using mist nets, and examined for the presence of nasal mites (identified according to specific keys) in the Pantanal, and Cerrado biomes, from the Mato Grosso state, midwestern Brazil. Overall, 76 Apodiform specimens, from the Trochilidae family were captured, and 20 female nasal mites, identified as being from the Rhinoseius genus, were collected from eight hosts (10.5%), of the following species: Amazilia fimbriata, Amazilia versicolor, Eupetonema macroura, and Thalurania furcata. The Rhinoseius spp. has been reported on, in many ecosystems in the Americas, including Brazil, however, this is the first report on Apodiformes from the Pantanal biome. Phoretic mites are not responsible for direct damage to the population of Apodiformes, as they are not pathogenic. However, they compete for food with hummingbirds and decrease the quantity of food available in the environment. They also affect the reproductive dynamics of plants, feed on pollen, and interfere with the intricacies of the biodiversity where they live.

5.
Arq. bras. med. vet. zootec ; 68(3): 571-578, tab, mapas
Artigo em Inglês | LILACS, VETINDEX | ID: lil-785708

RESUMO

The population of wild animals is regulated by many biotic and abiotic factors, and parasites are a biotic factor that affects the dynamic and density of host populations. From 2002 to 2014, 62 wild animals from the biomes Pantanal, Amazon, and "Cerrado" (or Savanna), which died in attendance in the veterinary hospital or have been road-killed, underwent necropsy for parasitological examination. Overall, 36 species of parasites were identified from 24 host species. Among the parasites, the most prevalent order was Oxyurida (29.1%), followed by Strongylida (20.9%), Spirurida (19.4%), Ascaridida (16.2%), Pentastomida (3.2%), Echinostomida (3.2%), Gygantorhynchia (3.2%), Rhabditida (1.6%), Plagiorchiida (1.6%), and Monilimorfida (1.6%), especially nematodes, which have more biotic potential and is more easily adapted to the environment than other classes. The occurrence of endoparasites was observed more frequently in endothermic than ectothermic animals, and herein is reported eleven new host occurrences for endoparasites in wild animals. The study has contributed to the knowledge on the biodiversity of parasites in wild animals from three biomes in central-western Brazil.(AU)


Populações de animais selvagens são reguladas por diversos fatores bióticos e abióticos, e parasitas são um fator biótico que afetam a dinâmica e a densidade de populações. De 2002 até 2014, 62 animais silvestres provenientes dos biomas Pantanal, Amazônia e Cerrado, que vieram a óbito no atendimento do hospital veterinário ou foram encontrados atropelados em rodovias, foram submetidos à necropsia parasitológica. Ao todo 36 espécies de parasitas foram identificadas em 24 espécies de hospedeiros. Entre os parasitas, a ordem mais prevalente foi Oxyurida (29,1%), seguida por Strongylida (20,9%), Spirurida (19,4%), Ascaridida (16,2%), Pentastomida (3,2%), Echinostomida (3,2%), Gygantorhynchia (3,2%), Rhabditida (1,6%), Plagiorchiida (1,6%) e Monilimorfida (1,6%), destacando-se os nematódeos, por seu maior potencial biótico e facilidade de adaptação ao meio do que as demais classes. A maior ocorrência de endoparasitas foi observada em animais endotérmicos que ectotérmicos, e este estudo registra onze novas ocorrências de hospedeiros para endoparasitas de animais selvagens. O estudo contribui para o conhecimento da biodiversidade de parasitas em animais silvestres dos três biomas do Centro-Oeste do Brasil.(AU)


Assuntos
Animais , Fatores Abióticos/análise , Animais Selvagens/parasitologia , Fatores Bióticos/análise , Nematoides , Parasitos , Biodiversidade , Conservação dos Recursos Naturais , Ecossistema , Doenças Parasitárias em Animais
6.
Med Vet Entomol ; 30(1): 112-6, 2016 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-26467462

RESUMO

Tick and blood samples collected from domestic dogs in the Brazilian Pantanal were tested by molecular methods for the presence of tick-borne protozoa and bacteria. Among 320 sampled dogs, 3.13% were infected by Babesia vogeli (Piroplasmida: Babesiidae), 8.75% by Hepatozoon canis (Eucoccidiorida: Hepatozoidae), 7.19% by Anaplasma platys (Rickettsiales: Anaplasmataceae), and 0.94% by an unclassified Anaplasma sp. In three tick species collected from dogs, the following tick-borne agents were detected: (a) B. vogeli, An. platys and Ehrlichia canis (Rickettsiales: Anaplasmataceae), infecting Rhipicephalus sanguineus sensu lato (Ixodida: Ixodidae) ticks; (b) H. canis, an unclassified Anaplasma sp. and Rickettsia amblyommii (Rickettsiales: Rickettsiaceae), infecting Amblyomma cajennense sensu lato (Ixodida: Ixodidae) ticks, and (c) Rickettsia sp. strain Atlantic rainforest, an emerging human pathogen, infecting Amblyomma ovale ticks. Molecular analysis, based on a mitochondrial gene, revealed that the Am. cajennense s.l. ticks of the present study corresponded to Amblyomma sculptum, a member of the Am. cajennense species complex, and that Rh. sanguineus s.l. belonged to the tropical lineage. Whereas dogs are exposed to a number of tick-borne bacterial and protozoan agents in the Pantanal biome, humans are potentially exposed to infection by spotted fever group rickettsiae (e.g. R. amblyommii and Rickettsia sp. strain Atlantic rainforest) because both Am. sculptum and Am. ovale are among the most important human-biting ticks in Brazil.


Assuntos
Doenças do Cão/epidemiologia , Carrapatos/microbiologia , Carrapatos/parasitologia , Animais , Brasil/epidemiologia , Doenças do Cão/microbiologia , Doenças do Cão/parasitologia , Cães , Feminino , Masculino
7.
Biomed Res Int ; 2013: 402737, 2013.
Artigo em Inglês | MEDLINE | ID: mdl-23936795

RESUMO

The distribution of Rickettsia parkeri in South America has been associated with Amblyomma triste ticks. The present study evaluated under laboratory conditions two colonies of A. triste: one started from engorged females that were naturally infected by R. parkeri (designated as infected group); the other started from noninfected females (designated as control group). Both colonies were reared in parallel for five consecutive generations. Tick-naïve domestic rabbits were used for feeding of each tick stage and generation. R. parkeri was preserved by transstadial maintenance and transovarial transmission in A. triste ticks for five consecutive generations, because all tested larvae, nymphs, and adults from the infected group were shown by PCR to contain rickettsial DNA. All rabbits infested by larvae, nymphs, and adults from the infected group seroconverted, indicating that these tick stages were all vector competent for R. parkeri. Expressive differences in mortality rates were observed between engorged nymphs from the infected and control groups, as indicated by 65.9% and 92.4% molting success, respectively. Our results indicate that A. triste can act as a natural reservoir for R. parkeri. However, due to deleterious effect caused by R. parkeri on engorged nymphs, amplifier vertebrate hosts might be necessary for natural long-term maintenance of R. parkeri in A. triste.


Assuntos
DNA Bacteriano/isolamento & purificação , Infecções por Rickettsia/transmissão , Rickettsia/patogenicidade , Carrapatos/genética , Animais , DNA Bacteriano/genética , Feminino , Humanos , Insetos Vetores/genética , Reação em Cadeia da Polimerase , Coelhos , Rickettsia/genética , Infecções por Rickettsia/genética , Carrapatos/patogenicidade
8.
Zoonoses Public Health ; 58(6): 416-23, 2011 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-21824336

RESUMO

Spotted fever is a disease caused by bacteria from the genus Rickettsia of the spotted fever group (SFG). Rickettsia rickettsii is likely the main agent of Brazilian spotted fever (BSF). With the objective of gathering information on the circulation of SFG rickettsiae in Londrina, Parana state, ticks from dogs and horses and also blood from dogs, horses and humans were collected in a neighbourhood of the city which presented potential for circulation of rickettsiae between hosts and vectors. Amblyomma cajennense, Dermacentor nitens, and Rhipicephalus sanguineus ticks were subjected to Polymerase Chain Reaction targeting a fragment of the Rickettsia gltA gene. This specific gene encodes the enzyme citrate synthase of Rickettsia spp., and results on all ticks were negative. Human and animal sera were tested by Indirect Immunofluorescence Assay in which R. rickettsii and R. parkeri were used as antigens. Sera from 4.7% human, 2.7% canine and 38.5% equine were positive for R. rickettsii. For R. parkeri, 0.9% human, 2.7% canine and 11.5% equine samples were positive. All samples reactive to R. parkeri also reacted to R. rickettsii. An epidemiological questionnaire was applied, but there were no statistically significant results. Comparison of our serological results with previous studies in Brazil, among BSF endemic and non-endemic areas, indicates that there is no established rickettsial infection in the study area, a statement corroborated with our molecular analysis. Nonetheless, as humans of the present study are highly exposed to tick infestations, health education within the population is needed to obtain efficient tick control.


Assuntos
Doenças do Cão/microbiologia , Doenças dos Cavalos/microbiologia , Infecções por Rickettsia/veterinária , Carrapatos/microbiologia , Zoonoses/epidemiologia , Adolescente , Adulto , Animais , Brasil/epidemiologia , Criança , Pré-Escolar , Doenças do Cão/epidemiologia , Cães , Feminino , Doenças dos Cavalos/epidemiologia , Cavalos , Humanos , Lactente , Recém-Nascido , Masculino , Pessoa de Meia-Idade , Infecções por Rickettsia/epidemiologia , Infecções por Rickettsia/microbiologia , Adulto Jovem
9.
Arq. bras. med. vet. zootec ; 63(2): 511-514, abr. 2011. tab
Artigo em Inglês | LILACS | ID: lil-591149

RESUMO

O presente estudo teve por objetivo avaliar, por imunofluorescência indireta, infecções ricketsiais em animais e humanos assintomáticos da área rural de Cerro Largo, RS. Dezesseis (51.6%) equinos, seis (22.3%) cães e oito (29.6%) humanos mostraram anticorpos reagentes (título <64) para Ricksettia rickettsii e para Ricksettia parkeri. Os estudos sorológicos indicaram que equinos, cães e humanos entraram em contato com rickettsias do grupo da febre maculosa nesse município. Dois cães mostraram títulos de R. parkeri no mínimo quatro vezes maior que os demais agentes ricketsiais testados e sugerindo que R. parkeri ou um genótipo próximo está circulando na região.


Assuntos
Humanos , Animais , Febre Maculosa das Montanhas Rochosas/patologia , Rickettsia/patogenicidade , Imunofluorescência , Sorologia/métodos
10.
Med Vet Entomol ; 25(2): 148-55, 2011 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-20950356

RESUMO

The present study was performed in an area endemic for Brazilian spotted fever (BSF) in Juiz de Fora, state of Minas Gerais, Brazil, during the years 2007 and 2008, when fatal cases of BSF (caused by Rickettsia rickettsii) were reported. Adult ticks (Acari: Ixodidae) identified as Rhipicephalus sanguineus (Latreille) and Amblyomma cajennense (Fabricius) were collected from dogs and horses, respectively, and tested by polymerase chain reaction (PCR). Overall, 13.1% of the Rh. sanguineus ticks and none of the A. cajennense were found to be infected with R. rickettsii. Two isolates of R. rickettsii were successfully established in Vero cell culture from two Rh. sanguineus ticks. An indirect immunofluorescence assay (IFA) using R. rickettsii antigens detected blood serological reaction to R. rickettsii in 67.9% (53/78) of dogs and 41.0% (16/39) of horses living in the study area. Larval offspring from two Rh. sanguineus engorged females, naturally infected by R. rickettsii, were reared to adult stage in the laboratory. All active stages (larvae, nymphs, adults) remained 100% infected by R. rickettsii, which was efficiently transmitted to naïve rabbits. Overall, the results of the present study indicate a potential risk for transmission of R. rickettsii to humans by Rh. sanguineus, an occurrence yet to be documented in Brazil.


Assuntos
Anticorpos Antibacterianos/sangue , Doenças do Cão/microbiologia , Doenças dos Cavalos/microbiologia , Ixodidae/microbiologia , Rickettsia rickettsii/isolamento & purificação , Febre Maculosa das Montanhas Rochosas/veterinária , Animais , Anticorpos Antibacterianos/imunologia , Brasil/epidemiologia , Células Cultivadas , Chlorocebus aethiops , Doenças do Cão/sangue , Doenças do Cão/epidemiologia , Cães , Feminino , Imunofluorescência , Doenças dos Cavalos/sangue , Doenças dos Cavalos/epidemiologia , Cavalos , Lagomorpha/sangue , Lagomorpha/microbiologia , Masculino , Reação em Cadeia da Polimerase , Rhipicephalus sanguineus/microbiologia , Rickettsia rickettsii/genética , Febre Maculosa das Montanhas Rochosas/sangue , Febre Maculosa das Montanhas Rochosas/epidemiologia , Febre Maculosa das Montanhas Rochosas/microbiologia , Células Vero
11.
Arq. bras. med. vet. zootec ; 62(4): 1007-1010, Aug. 2010. tab
Artigo em Português | LILACS | ID: lil-562074

RESUMO

A cross-sectional study was conducted to determine the seroprevalence of anti-Rickettsia spp. in dogs from Belo Horizonte, MG. For this purpose, serum samples from 453 dogs were collected during the rabies vaccination campaign and tested by the indirect immunofluorescence assay (IFA) using five antigens: Rickettsia bellii, Rickettsia amblyommii, Rickettsia rickettsii, Rickettsia parkeri, and Rickettsia felis. The endpoint titer reacting with each antigen was determined and serum was considered positive if it reacted at the 1:64 dilution. Serum showing titer for a Rickettsia species at least four-fold higher than that observed for any other Rickettsia species was considered homologous to the first Rickettsia species. Only three (0.66%) dogs reacted positively to at least one Rickettsia species and one serum showed to be homologous to R. rickettsii. These results showed a low prevalence of antibodies anti-Rickettsia spp. in dogs from Belo Horizonte city. However, other serosurvey needs to be performed for surveillance of the endemic status of the disease in the municipal district.


Assuntos
Animais , Cães , Rickettsia/imunologia , Estudos Soroepidemiológicos , Brasil/epidemiologia , Cães
12.
Arq. bras. med. vet. zootec ; 62(3): 761-764, June 2010.
Artigo em Português | LILACS | ID: lil-554951

RESUMO

The Brazilian Spotted Fever (BSF) is a zoonosis that can be fatal if not trteated. As there are few studies of the BSF in the Paraná State, the occurrence of BSF was serologically investigated in dogs and horses by indirect immunofluorescence assay (IFA), in the North of that State, in animals that are regarded as sentinels for BSF. A total of 241 samples of sera of equine, four of asinine, and 29 of dogs were collected in nine farms of three municipalities in the North of Paraná: Arapongas, Douradina, and Umuarama. From fifteen samples of equine serum with positive titres for RIFI, eight (53.3 percent) had titre of 64 against R. rickettsii, two (13.3 percent) 128 against R. rickettsii, and five (33.3 percent) were reactants for R. parkeri and R. rickettsii, with titres ranging from 64 to 2048, and 128 to 1024, respectively. The results showed that domestic animals, sentinels for BSF, are under low exposition to ticks infected with spotted fever group Rickettsia, indicating low risk of human infection by these agents in the studied area.


Assuntos
Animais , Cães , Cães/imunologia , Cavalos/imunologia , Rickettsia/isolamento & purificação , Infecções por Rickettsiaceae
13.
Ann Trop Med Parasitol ; 103(5): 413-25, 2009 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-19583912

RESUMO

The rickettsial infections in 174 Amblyomma nodosum found on passeriform birds in the Atlantic forest, eastern Brazil, have recently been evaluated. Rickettsiae were successfully isolated from two ticks, using cultures of Vero cells. Both isolates were molecularly characterised, using the rickettsial genes gltA and htrA and, when possible, also ompA and ompB. Portions of the gltA and htrA genes from one of the rickettsial isolates were found be closely match the corresponding GenBank sequences for Rickettsia bellii, with 99.9% and 100% homology, respectively. This isolate was named R. bellii strain Pontal. Portions of the gltA, htrA and ompB genes from the second isolate most closely matched the corresponding sequences of R. parkeri, whereas a portion of the ompA gene from this isolate was closest to the relevant sequence of Rickettsia sp. strain COOPERI (which has been considered to be a strain of R. parkeri in Brazil). The second isolate was named R. parkeri strain NOD. Further investigation of the 172 ticks from which isolates were not recovered revealed R. parkeri strain NOD in 40 and R. bellii strain Pontal in nine, giving overall infection prevalences of 23.6% (41/174) and 5.7% (10/174), respectively. This appears to be the first report of R. bellii and R. parkeri in A. nodosum.


Assuntos
Doenças das Aves/microbiologia , Passeriformes/parasitologia , Infecções por Rickettsia/epidemiologia , Rickettsia/isolamento & purificação , Infestações por Carrapato/veterinária , Carrapatos/microbiologia , Animais , Vetores Aracnídeos/genética , Vetores Aracnídeos/microbiologia , Brasil/epidemiologia , DNA Bacteriano/genética , DNA Bacteriano/isolamento & purificação , Ixodidae/genética , Ixodidae/microbiologia , Dados de Sequência Molecular , Reação em Cadeia da Polimerase , Rickettsia/genética , Infecções por Rickettsia/genética , Infestações por Carrapato/microbiologia , Carrapatos/genética
14.
Ann Trop Med Parasitol ; 103(1): 57-72, 2009 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-19173777

RESUMO

In a recent ecological study of the ticks on animal trails within an area of Atlantic rainforest in south-eastern Brazil, Amblyomma aureolatum, A. brasiliense, A. incisum, A. ovale and Haemaphysalis juxtakochi were found questing on the vegetation. Most of the ticks recorded by a small, man-made dam on the forest border were A. dubitatum but a few A. brasiliense and A. cajennense, one A. incisum and one H. juxtakochi were also found. The seasonal activity of the ticks indicated that A. incisum and A. brasiliense had one generation/year. On the animal trails, most tick species and stages quested on the vegetation at a height of 30-40 cm above ground level. The questing larvae and adults of A. incisum tended to be found higher, however, with the greatest numbers recorded 40-50 cm (larvae) or 60-70 cm (adults) above ground level. Most of the adult ticks (81.1%-100%), nymphs (78.6%-100%) and larval clusters (100%) found on a forest trail remained questing at the same location over a 24-h period. Carbon-dioxide traps in the rainforest attracted <50% of the ticks observed questing on the nearby vegetation and, curiously, the CO2 traps set deep in the forest attracted far fewer ticks than similar traps set by the dam. The ecological relationships between the ticks, their hosts and the rainforest environment are discussed.


Assuntos
Animais Selvagens/parasitologia , Ixodidae/classificação , Mamíferos/parasitologia , Infestações por Carrapato/epidemiologia , Animais , Mordeduras e Picadas , Brasil/epidemiologia , Humanos , Ixodidae/crescimento & desenvolvimento , Infestações por Carrapato/veterinária
15.
Vet Microbiol ; 87(2): 131-8, 2002 Jun 20.
Artigo em Inglês | MEDLINE | ID: mdl-12034541

RESUMO

Antigenic characterization of A. marginale isolates has contributed to identifying the presence of common and restricts epitopes of major surface proteins (MSPs). The data may improve vaccine development to protect against A. marginale isolates from different regions. Brazilian A. marginale isolates were characterized antigenically by Western blot with monoclonal antibodies (MAbs) against MSPs and rabbit anti-MSP-4 from Florida strain. Six A. marginale isolates from MS, MG (AUFV1), SP, PR-L1, PR-HV, RS and Florida strain were tested with ANA22B1 to MSP-1a, AMR36A6 to MSP-1b, ANAF19E2 to MSP-2, AMG75C1 and AMG76B2 to MSP-3 and ANAF16C1 to MSP-5. ANA22B1 recognized MSP-1a epitope in all A. marginale isolates, and reacted with polypeptides of different size ranging 46-105kDa. MSP2 was not detected in MS and SP isolates by ANAF19E2, and only PR-L1 and MG (AUFV1) isolates reacted with MAbs which recognize MSP3 epitope. MSP4 and MSP5 were detected in all A. marginale isolates analyzed. The results revealed conservation of MSP-1a and MSP-5 epitopes among all Brazilian isolates, and showed antigenic variability to MSP-1b, MSP-2 and MSP-3 proteins, agreeing with recent data about the genetic diversity found in the polimorphic multigene family responsible for these proteins.


Assuntos
Anaplasma/imunologia , Anaplasmose/imunologia , Variação Antigênica/imunologia , Antígenos de Bactérias/genética , Doenças dos Bovinos/imunologia , Anaplasma/genética , Animais , Anticorpos Monoclonais , Antígenos de Bactérias/imunologia , Western Blotting/veterinária , Brasil , Bovinos , Doenças dos Bovinos/microbiologia
16.
Arq. bras. med. vet. zootec ; 53(5): 553-556, out. 2001. tab
Artigo em Português | LILACS | ID: lil-307908

RESUMO

During January and February, 2000, 133 dogs from 21 farms located in eight diferent counties at the northern region of Paraná, Brazil, were examined for ticks. Ticks found on dogs were collected and identified. Information about dog rearing system was also obtained. Fifty-five ticks were collected on 19 dogs (14.3 per cent) from 11 farms (52.4 per cent). Four species were identified: Rhipicephalus sanguineus (20 ticks on nine dogs from seven farms), Boophilus microplus (15 ticks on five dogs from five farms), Amblyomma ovale (nine ticks on six dogs from four farms) and Amblyomma aureolatum (11 ticks on three dogs from one farm). The results are discussed considering the behavior of some dogs used to stroll into the woods and pasture areas whereas other dogs remained closer to the human facilities


Assuntos
Cães , Parasitologia , Carrapatos
17.
Biosystems ; 41(2): 127-37, 1997.
Artigo em Inglês | MEDLINE | ID: mdl-9043681

RESUMO

The traditional iterated prisoner's dilemma (IPD) admits only two possible moves, cooperate (C) and defect (D), with no gradations involved. Nevertheless, when a rational agent perceives or implements a specific behavioral decision, it usually employs qualitative measures of the variables involved. In this paper, we propose an approach to the IPD where the possible moves are still confined to C and D, but these are no longer considered as two dichotomous choices, but as different attitudes that can have variable emphasis. The variables accounted for in the game are modeled as fuzzy sets, and the players' decisions are taken with the guidance of fuzzy expert systems. A computational tournament is performed, where in addition to the fuzzy players, the well-known successful strategists tit for tat (TFT) and Pavlov are also present. Some results are presented and briefly discussed. The main purpose of this paper is to investigate a model of the IPD in which the players' decisions are taken by means of a qualitative reasoning system.


Assuntos
Simulação por Computador , Teoria da Decisão , Animais , Humanos
18.
Rev. Col. Bras. Cir ; 11(3): 68-73, 1984.
Artigo em Português | LILACS | ID: lil-23372

RESUMO

Tratamento cirurgico de megaesofago pela cardiectomia com interposicao de alca jejunal exclusa. Avaliacao clinica dos resultados. Os autores analisam os resultados obtidos com o emprego da operacao de Merendino no tratamento cirurgico do megaesofago em 36 doentes. A motalidade pos-operatoria foi de 8,33%. Foram seguidos no pos-operatorio 28 doentes (84,85%).Houve alivio da disfagia em 96,43%.Apesar dos resultados, os autores concluem que a operacao de Merendino deva ser reservada a casos nos quais formas mais simples de tratamento tenham sido previamente tentadas


Assuntos
Adulto , Pessoa de Meia-Idade , Humanos , Masculino , Feminino , Acalasia Esofágica , Jejuno , Procedimentos Cirúrgicos Operatórios
19.
Rev. Col. Bras. Cir ; 10(1): 24-8, 1983.
Artigo em Português | LILACS | ID: lil-14908

RESUMO

Os autores analisam 20 casos de hernia incisional abdominal, tratados pela tecnica de superposicao peritonioaponeurotica proposta por Lazaro da Silva. Descrevem a tecnica e comentam seus resultados imediatos


Assuntos
Adulto , Pessoa de Meia-Idade , Humanos , Masculino , Feminino , Cicatriz , Hérnia , Procedimentos Cirúrgicos Operatórios
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