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1.
J Emerg Manag ; 17(5): 371-379, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31736043

RESUMO

Emergency management has traditionally been led by an attitude to react and respond and implement effective recovery efforts. The focus has now shifted to a preparedness perspective, promoting measures which encourage the public and responders to be better equipped leading to an increased level of resiliency. Effective use of Recreation-based stakeholders and facilities, in emergency planning, boasts a number of positive elements to all phases of emergency management. A literature review will focus on the importance of Recreation in relation to preparedness. Findings will seek to articulate how the involvement of Recreation, in the realm of preparedness, builds community resilience, enhances response and recovery operations and provides a unique leadership and knowledge base regarding the use of recreational infrastructure. This effort in turn leads to a community more capable of managing an incident and returning to normalcy.


Assuntos
Defesa Civil , Planejamento em Desastres , Emergências , Recreação , Canadá , Humanos
2.
Toxicology ; 314(2-3): 262-6, 2013 Dec 15.
Artigo em Inglês | MEDLINE | ID: mdl-23831209

RESUMO

Polyethylene glycols (PEGs) are commonly employed as excipients in preclinical studies and in vitro experiments to dissolve poorly hydrosoluble drugs. Their use is generally considered safe in both animals and humans; however, limited data is available concerning the safety of PEGs when administered parenterally. The results of our investigation demonstrate that PEG-400 can have an irritant effect on serosal surfaces and causes subcapsular hepatocellular necrosis in mice when administered intraperitoneally at a high dose (4 mL/kg). Accordingly, levels of serum biomarkers of liver injury need to be carefully interpreted in studies where PEG is administered intraperitoneally and always in association with the results of the histological assessment.


Assuntos
Doença Hepática Induzida por Substâncias e Drogas/patologia , Peritonite/induzido quimicamente , Peritonite/patologia , Polietilenoglicóis/toxicidade , Animais , Biomarcadores/sangue , Doença Hepática Induzida por Substâncias e Drogas/sangue , Feminino , Células Hep G2 , Humanos , Injeções Intraperitoneais , Fígado/efeitos dos fármacos , Fígado/metabolismo , Fígado/patologia , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Necrose/sangue , Necrose/induzido quimicamente , Necrose/patologia , Peritonite/sangue , Polietilenoglicóis/administração & dosagem , Estudos Retrospectivos
3.
Biochim Biophys Acta ; 1770(8): 1240-7, 2007 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-17560037

RESUMO

The glutathione transferases (GSTs) mediate the detoxification of a broad spectrum of electrophilic chemicals. We report here the identification and characterisation of a novel naturally occurring transition that changes codon 169 from GGC (Gly) to GAC (Asp) in the human Pi class GST, GSTP1. Expression of the variant in human HepG2 cells led to a small increase in 1-chloro-2,4-dinitrobenzene (CDNB) conjugation compared to the wild-type protein. Asp(169) GSTP1-1 expressed at high levels in Escherichia coli displayed a small but significant increase in specific activity towards CDNB compared to Gly(169) GSTP1-1. The catalytic efficiency with CDNB was higher for Asp(169) GSTP1-1 compared to the wild-type enzyme, although the kinetic constants of the mutant and the wild-type enzyme towards glutathione were not different. Modelling indicated that the mutation does not appear to change protein conformation. The distribution of the genotypes in a normal healthy population (217 individuals) was 94.3% for the Gly/Gly genotype and 5.7% for the Gly/Asp genotype; no Asp/Asp genotypes were detected in this population. The frequency of the Asp(169) allele in the only oxidative stress-linked pathology that we have studied to date, i.e. alcoholic liver disease, was not significantly different from healthy controls. In conclusion, we have detected and characterised a novel SNP in GSTP1 that may play a role in modulating the activity of GSTP1-1.


Assuntos
Glutationa S-Transferase pi/genética , Glutationa S-Transferase pi/metabolismo , Polimorfismo Genético , Alelos , Substituição de Aminoácidos , Ácido Aspártico/metabolismo , Carcinoma Hepatocelular/patologia , Linhagem Celular Tumoral , Dicroísmo Circular , Dinitroclorobenzeno/metabolismo , Escherichia coli/genética , Frequência do Gene , Genótipo , Glutationa S-Transferase pi/química , Glutationa Transferase/classificação , Glutationa Transferase/metabolismo , Humanos , Cinética , Hepatopatias Alcoólicas/genética , Hepatopatias Alcoólicas/patologia , Neoplasias Hepáticas/patologia , Modelos Moleculares , Estrutura Secundária de Proteína , Proteínas Recombinantes/química , Proteínas Recombinantes/metabolismo , Temperatura Ambiente
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