Your browser doesn't support javascript.
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 30
Filtrar
Filtros adicionais











Intervalo de ano
1.
Colloids Surf B Biointerfaces ; 184: 110521, 2019 Sep 23.
Artigo em Inglês | MEDLINE | ID: mdl-31569001

RESUMO

Titanium dioxide nanotube arrays (TNTAs) have attracted extensive attention in the fields of biomaterials and biomedicine due to their unique tubular structure and good biocompatibility. In this paper, TNTAs with different nanotube diameters and lengths were in situ prepared on the titanium surface by the anodic oxidation, and their crystal structures were further changed by annealing treatment. The effects of TNTAs with different diameters and crystals on the blood compatibility and endothelial cell behaviors were investigated. The results showed that TNTAs with the diameter of 30∼90 nm can be obtained by controlling the anodization voltage, and annealing treatment did not obviously change the diameters and lengths of the nanotube arrays. However, annealing treatment can transform the amorphous TNTAs into the anatase structure. The diameter and crystal structure of the nanotube arrays played a key role in the surface wettability and protein adsorption. The nanotube array with larger diameter displayed better surface hydrophilicity as compared to the pristine titanium, and annealing treatment further enhanced the hydrophilicity. As compared to the pristine titanium, the nanotube array structure had the characteristic of selective protein adsorption, and the nanotube array can promote the bovine serum albumin (BSA) adsorption and prevent the fibrinogen (FIB) adsorption, however, the increase of nanotube diameter could reduce BSA adsorption and increase FIB adsorption. Besides, the nanotube array with anatase structure can promote BSA adsorption while reduce FIB adsorption. Therefore, the TNTAs with smaller diameter and anatase crystal had good blood compatibility and cell compatibility, they can not only reduce platelet adhesion and hemolysis rate but also increase endothelial cell adhesion and proliferation. In conclusion, the nanotube arrays of the present study can be used to improve the cell compatibility and blood compatibility of the titanium implants.

2.
Mater Sci Eng C Mater Biol Appl ; 104: 109947, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31499970

RESUMO

Due to its good biodegradability and mechanical properties, magnesium alloys are considered as the ideal candidate for the cardiovascular stents. However, the rapid degradation in human physiological environment and the poor biocompatibility seriously limit its application for biomaterials. In the present study, a chitosan/heparinized graphene oxide (Chi/HGO) multilayer coating was constructed on the AZ31B magnesium alloy surface using layer-by-layer (LBL) method to improve the corrosion resistance and biocompatibility. The results of attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR), Raman spectrum (RAMAN), scanning electron microscopy (SEM) and energy dispersive spectroscopy (EDS) showed that a dense and compact Chi/HGO multilayer coating was fabricated on the magnesium alloy surface. The results of potentiodynamic polarization, electrochemical impedance spectroscopy (EIS), pH value changes and magnesium ion release suggested that the multilayer coating can significantly enhance the corrosion resistance of the magnesium alloy. Moreover, the Chi/HGO multilayer coating could not only significantly reduce the hemolysis rate and platelet adhesion, but also promote the adhesion and proliferation of endothelial cells. Therefore, the Chi/HGO multilayer coating can simultaneously improve the corrosion resistance and biocompatibility of the magnesium alloys.

3.
Chem Commun (Camb) ; 55(59): 8564-8566, 2019 Jul 18.
Artigo em Inglês | MEDLINE | ID: mdl-31271158

RESUMO

Diphenylalanine (FF), as the smallest unit and core recognition motif of ß-amyloid (Aß), could self-assemble into nanofibers, which induces an early onset of Alzheimer's disease (AD). Green/near-infrared fluorescent BODIPY probes were designed and synthesized to detect FF-assembly, providing unique insights into the chemical and molecular mechanism of Aß aggregation and drug development for AD.


Assuntos
Compostos de Boro/química , Corantes Fluorescentes/química , Fenilalanina/análogos & derivados , Técnicas Biossensoriais/métodos , Compostos de Boro/síntese química , Corantes Fluorescentes/síntese química , Nanofibras/química , Fenilalanina/análise , Fenilalanina/química , Multimerização Proteica
4.
Colloids Surf B Biointerfaces ; 173: 884-890, 2019 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-30551305

RESUMO

Application of organic dyes is limited in biomedical fields due to the rapid self-quenching, poor stability and water solubility. In this work, polymer modified organic dyes is developed through the dyes initiating polymerization. The polymer length is studied by tuning the amount of monomer. With an optimal molecule weight of the polymer, the π-π stacking from π-conjugated organic fluorescent dyes and fluorescence quenching are inhibited, resulting in an enhancement of fluorescence intensity and photostability. Nanoparticles are further fabricated to be used for cell uptake based on the optimized organic dyes. Then, long term cellular fluorescence imaging is realized. This work highlights the potential of polymer modification to improve the performance of organic dyes and expand their applications.


Assuntos
Microscopia de Fluorescência , Nanopartículas/química , Compostos Orgânicos/química , Poliésteres/química , Células A549 , Endocitose , Corantes Fluorescentes/química , Humanos , Espectroscopia de Ressonância Magnética , Microscopia Confocal , Nanotecnologia , Fotoquímica
5.
ACS Appl Mater Interfaces ; 10(47): 40844-40853, 2018 Nov 28.
Artigo em Inglês | MEDLINE | ID: mdl-30403339

RESUMO

The development of a facile and versatile strategy to endow surfaces with synergistically anti-inflammatory, antimicrobial, and anticoagulant functions is of particular significance for blood-contacting biomaterials and medical devices. In this work, we report a simple and environmentally friendly "one-pot" method inspired by byssal cuticle chemistry, namely, [Fe(dopa)3] coordination chemistry for assembly of copper ions (Cu2+) and plant polyphenol (tannic acid)/catecholamine (dopamine or norepinephrine) to form metal-phenolic/catecholamine network-based coatings. This one-pot method enabled us to easily develop a multifunctional surface based on the combination of the characteristic functions of metal ions and plant polyphenol or catecholamine. The residual phenolic hydroxyl groups on the coatings imparted the modified surface with excellent antioxidant and anti-inflammatory functions. The robust chelation of copper ions to the metal-phenolic/catecholamine networks provided not only durable antibacterial property but also glutathione peroxidase like catalytic capability to continuously and controllably produce antithrombotic nitric oxide by catalyzing endogenous S-nitrothiol. The biological functions of such coatings could be well regulated by adjusting the ratios of the feed concentration of Cu2+ ions to plant polyphenol or catecholamine. We envision that our simple, multifunctional, and bioinspired coating strategy can hold great application promise for bioengineering blood-contacting devices.

6.
Int J Biol Macromol ; 120(Pt B): 1396-1405, 2018 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-30266642

RESUMO

Heparin-like membranes (CPBS) with nanofibers (approximate diameters of 100-500 nm) were prepared through electrospinning of a blended solution of carboxymethyl chitosan nanoparticle (CMCN, diameters 483 nm) and poly (vinyl alcohol) (CMCN/PVA) onto the surface of modified bacterial cellulose sulfate (BCS) membranes. SEM images confirmed that the CMCN were stretched to nanofibers during electrospinning. The presence of BCS on the collector of electrospinning machine increased the spinnability of CMCN/PVA solution. FTIR and XPS measurement revealed that there were SO3-, COO-, and OH groups on the surface of CPBS membrane, expressing structural similarity to heparin. CPBS membranes maintained hydrophilicity and the glutaraldehyde crosslinked CPBS membrane was stable in water. The clotting time and platelet adhesion experiments expressed the anticoagulant properties of CPBS. The APTT, TT and PT of CPBS increased up to 116.0%, 189.8%, and 50% than those of the plasma, (67.4 s, 16.2 s, and 48.4 s, respectively). No platelets adhered onto the surface of CPBS. An inflammatory response was determined according to activation of the macrophages seeded onto the membranes.

7.
Colloids Surf B Biointerfaces ; 161: 156-161, 2018 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-29078164

RESUMO

In this work, permeability of polymersomes was quantified by fluorescence enhancement. The polymersomes were prepared from poly(ethylene glycol)-b-poly(ε-caprolactone) (PEG-PCL), and an acid-sensitive hydrophobic dye borondipyrromethene (BODIPY) was encapsulated into the membrane of polymersomes. H+ from trifluoroacetic acid (TFA) would diffuse into the membrane of polymersomes to enhance the fluorescence of BODIPY. Intensity of the enhanced fluorescence could be used for quantifying the H+ permeability of polymersomes. In addition, poly(ε-caprolactone) (PCL) was used to control the permeability ability of the membrane. Such quantitative information on the molecular diffusion of polymersomes is of fundamental importance to many of their potential applications, e.g., nanoreactors and drug delivery.


Assuntos
Compostos de Boro/química , Permeabilidade da Membrana Celular , Etilenoglicóis/química , Membranas Artificiais , Poliésteres/química , Portadores de Fármacos/química , Sistemas de Liberação de Medicamentos , Fluorescência , Interações Hidrofóbicas e Hidrofílicas , Microscopia Eletrônica de Transmissão , Permeabilidade
8.
Biomater Sci ; 5(12): 2416-2426, 2017 Nov 21.
Artigo em Inglês | MEDLINE | ID: mdl-29115308

RESUMO

It has been widely recognized that functional groups on biomaterial surfaces play important roles in blood compatibility. To construct an effective antithrombotic bio-interface onto the poly(ether sulfone) (PES) membrane surface, bio-functional groups of sodium carboxylic (-COONa), sodium sulfonic (-SO3Na) and amino (-NH2) groups were introduced onto the PES membrane surface in three steps: the synthesis of PES with carboxylic (-COOH) groups (CPES) and water-soluble PES with sodium sulfonic (-SO3Na) groups and amino (-NH2) groups (SNPES); the introduction of carboxylic groups onto the PES membrane by blending CPES with PES; and the grafting of SNPES onto CPES/PES membranes via the coupling of amino groups and carboxyl groups. The physical/chemical properties and bioactivities were dependent on the proportions of the additives. After introducing bio-functional groups, the excellent hemocompatibility of the modified membranes was confirmed by the inhibited platelet adhesion and activation, prolonged clotting times, suppressed blood-related complement and leukocyte-related complement receptor activations. Furthermore, cell tests indicated that the modified membranes showed better cytocompatibility in endothelial cell proliferation than the pristine PES membrane due to the synergistic promotion of the functional groups. To sum up, these results suggested that modified membranes present great potential in fields using blood-contacting materials, such as hemodialysis and surface endothelialization.


Assuntos
Materiais Biocompatíveis/química , Plásticos Biodegradáveis/síntese química , Fibrinolíticos/síntese química , Polímeros/química , Sulfonas/química , Materiais Biocompatíveis/síntese química , Materiais Biocompatíveis/uso terapêutico , Plásticos Biodegradáveis/química , Plásticos Biodegradáveis/uso terapêutico , Proliferação de Células/efeitos dos fármacos , Células Endoteliais/efeitos dos fármacos , Fibrinolíticos/química , Fibrinolíticos/uso terapêutico , Humanos , Membranas Artificiais , Ativação Plaquetária/efeitos dos fármacos , Adesividade Plaquetária/efeitos dos fármacos , Polímeros/síntese química , Polímeros/uso terapêutico , Diálise Renal/métodos , Sulfonas/síntese química , Sulfonas/uso terapêutico , Propriedades de Superfície
9.
ACS Appl Mater Interfaces ; 9(36): 30373-30386, 2017 Sep 13.
Artigo em Inglês | MEDLINE | ID: mdl-28816035

RESUMO

Surface biomimetic modification with extra-cellular matrix (ECM)-derived biomolecules is an emerging potential method of accelerating the healing of vascular stent lesions. However, insufficient capacity of the constructed biofunctional layer in maintaining its long-term efficiency and preventing thrombus and neointimal hyperplasia continue to be major limitations in clinical application. On the basis of the structure and function of ECM, in this study, we constructed a novel stromal cell-derived factor-1α (SDF-1α)/laminin-loaded nanocoating on the 316L stainless steel (SS) surface to provide improved function in modulation of vascular remodeling. The modified surface was found to control delivery of biomolecules and exhibit promising potential to provide stage-adjusted treatment after injury. An in vitro biocompatibility study suggested that the constructed layer may effectively prevent thrombosis formation by inhibiting platelet adhesion and activation, while accelerating endothelium regeneration by inducing endothelial cell (EC) migration and endothelial progenitor cell (EPC) aggregation. An in vivo animal test further demonstrated that the nanocoating may prevent thrombus and neointimal hyperplasia after implantation for 3 months. Therefore, the ECM-inspired nanocoating described in this study is a promising novel approach for vascular stent surface modification.

10.
Mater Sci Eng C Mater Biol Appl ; 70(Pt 1): 438-449, 2017 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-27770914

RESUMO

In recent years, magnesium alloys are attracting more and more attention as a kind of biodegradable metallic biomaterials, however, their uncontrollable biodegradation speed in vivo and the limited surface biocompatibility hinder their clinical applications. In the present study, with the aim of improving the corrosion resistance and biocompatibility, the magnesium alloy (AZ31B) surface was modified by alkali heating treatment followed by the self-assembly of 3-aminopropyltrimethoxysilane (APTMS). Subsequently, poly (ethylene glycol) (PEG) and fibronectin or fibronectin/heparin complex were sequentially immobilized on the modified surface. The results of attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR) and X-ray photoelectron spectroscopy (XPS) confirmed that the above molecules were successfully immobilized on the magnesium alloy surface. An excellent hydrophilic surface was obtained after the alkali heating treatment while the hydrophilicity decreased to some degree after the self-assembly of APTMS, the surface hydrophilicity was gradually improved again after the immobilization of PEG, fibronectin or fibronectin/heparin complex. The corrosion resistance of the control magnesium alloy was significantly improved by the alkali heating treatment. The self-assembly of APTMS and the following immobilization of PEG further enhanced the corrosion resistance of the substrates, however, the grafting of fibronectin or fibronectin/heparin complex slightly lowered the corrosion resistance. As compared to the pristine magnesium alloy, the samples modified by the immobilization of PEG and fibronectin/heparin complex presented better blood compatibility according to the results of hemolysis assay and platelet adhesion as well as the activated partial thromboplastin time (APTT). In addition, the modified substrates had better cytocompatibility to endothelial cells due to the improved anticorrosion and the introduction of fibronectin. The substrates modified by fibronectin or fibronectin/heparin complex can significantly promote endothelial cell adhesion and proliferation. Taking all these results into consideration, the method of the present study can be used for the surface modification of the magnesium alloy to simultaneously impart it better corrosion resistance, favorable blood compatibility and good cytocompatibility to endothelial cells.


Assuntos
Álcalis/química , Ligas/farmacologia , Fibronectinas/farmacologia , Calefação , Heparina/farmacologia , Magnésio/farmacologia , Teste de Materiais/métodos , Polietilenoglicóis/farmacologia , Anticoagulantes/farmacologia , Plaquetas/efeitos dos fármacos , Plaquetas/ultraestrutura , Adesão Celular/efeitos dos fármacos , Proliferação de Células/efeitos dos fármacos , Corrosão , Células Endoteliais/citologia , Células Endoteliais/efeitos dos fármacos , Células Endoteliais/ultraestrutura , Hemólise/efeitos dos fármacos , Humanos , Concentração de Íons de Hidrogênio , Interações Hidrofóbicas e Hidrofílicas , Proteínas Imobilizadas/farmacologia , Adesividade Plaquetária/efeitos dos fármacos , Polietilenoglicóis/química , Soluções , Espectrometria por Raios X , Espectroscopia de Infravermelho com Transformada de Fourier , Propriedades de Superfície , Água/química
11.
Mater Sci Eng C Mater Biol Appl ; 71: 929-936, 2017 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-27987790

RESUMO

Late thrombus and restenosis caused by delayed endothelialization and insufficient biocompatibility of polymer coating continue to be the greatest limitations of drug-eluting stents. In this study, based on the specific structure of vascular basement membrane, a novel biomimetic nano-coating was constructed by incorporating laminin into electrostatic-assembled heparin/poly-l-lysine nanoparticles. Alteration of heparin and poly-l-lysine concentration ratio in a certain range has no significantly influence nanoparticle size, uniformity and stability, but may affect the chemical property and subsequently the binding efficiency to dopamine-coated titanium surface. By use of this feature, four different nanoparticles were synthesized and immobilized on titanium surface for creating gradient nanoparticle binding density. According to in vitro biocompatibility evaluation, the nanoparticle modified surfaces were found to effectively block coagulation pathway and reduce thrombosis formation. Moreover, NP10L and NP15L modified surface with relatively low heparin exposing density (4.9 to 7.1µg/cm2) showed beneficial effect in selective promoting EPCs and ECs proliferation, as well as stimulating cell migration and NO synthesis.


Assuntos
Materiais Biomiméticos , Plaquetas/metabolismo , Heparina , Laminina , Teste de Materiais , Nanopartículas/química , Polilisina , Materiais Biomiméticos/química , Materiais Biomiméticos/farmacologia , Heparina/química , Heparina/farmacologia , Humanos , Laminina/química , Laminina/farmacologia , Adesividade Plaquetária/efeitos dos fármacos , Polilisina/química , Polilisina/farmacologia , Propriedades de Superfície
12.
Mater Sci Eng C Mater Biol Appl ; 69: 1175-82, 2016 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-27612815

RESUMO

Bio-inorganic films and drug-eluting coatings are usually used to improve the hemocompatibility and inhibit restenosis of vascular stent; however, above bio-performances couldn't combine together with single materials. In the present study, we reported a simple approach to fabricate a metal film with the aim of imparting the stent with good blood compatibility and accelerating endothelialization. The films with various ratios of Cu and Ti were prepared through the physical vapor deposition. Phase structure and element composition were investigated by X-ray diffraction (XRD) and X-ray photoelectron spectroscopy (XPS), respectively. The releasing volume of copper ion in Cu/Ti film was determined by immersing test. The hemolysis ratio, platelet adhesion and clotting time were applied to evaluate the hemocompatibility. The proliferative behaviors of endothelial cells and smooth muscle cells under certain copper concentration were investigated in vitro and in vivo. Results indicated that copper-titanium films exhibited good hemocompatibility in vitro; however, the increase of Cu/Ti ratio could lead to increasing hemolysis ratio. Endothelial cells displayed more proliferative than smooth muscle cells when the copper concentration was <7.5µg/ml, however both cells tended to apoptosis to some degree when the copper concentration was increased. The complete endothelialization of the film with low copper in vivo was observed at the 2nd week, indicating that the copper-titanium film with the lower copper concentration could promote endothelialization. Therefore, the inorganic copper-titanium film could be potential biomaterials to improve blood compatibility and accelerating endothelialization of vascular stents.


Assuntos
Materiais Revestidos Biocompatíveis/química , Cobre/química , Stents , Titânio/química , Animais , Aorta/efeitos dos fármacos , Aorta/metabolismo , Adesão Celular/efeitos dos fármacos , Eritrócitos/citologia , Eritrócitos/efeitos dos fármacos , Hemólise/efeitos dos fármacos , Células Endoteliais da Veia Umbilical Humana , Humanos , Espectroscopia Fotoeletrônica , Adesividade Plaquetária/efeitos dos fármacos , Coelhos , Aço Inoxidável/química , Propriedades de Superfície , Molhabilidade , Difração de Raios X
13.
Mater Sci Eng C Mater Biol Appl ; 67: 132-143, 2016 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-27287107

RESUMO

Magnesium based alloys are attracting tremendous interests as the novel biodegradable metallic biomaterials. However, the rapid in vivo degradation and the limited surface biocompatibility restrict their clinical applications. Surface modification represents one of the important approaches to control the corrosion rate of Mg based alloys and to enhance the biocompatibility. In the present study, in order to improve the corrosion resistance and surface biocompatibility, magnesium alloy (AZ31B) was modified by the alkali heating treatment followed by the self-assembly of 3-phosphonopropionic acid, 3-aminopropyltrimethoxysilane (APTMS) and dopamine, respectively. The results of attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR) and X-ray photoelectron spectra (XPS) indicated that the molecules were successfully immobilized on the magnesium alloy surface by the self-assembly. An excellent hydrophilic surface was obtained after the alkali heating treatment and the water contact angle increased to some degree after the self-assembly of dopamine, APTMS and 3-phosphonopropionic acid, however, the hydrophilicity of the modified samples was better than that of the pristine magnesium substrate. Due to the formation of the passivation layer after the alkali heating treatment, the corrosion resistance of the magnesium alloy was obviously improved. The corrosion rate further decreased to varying degrees after the self-assembly surface modification. The blood compatibility of the pristine magnesium was significantly improved after the surface modification. The hemolysis rate was reduced from 56% of the blank magnesium alloy to 18% of the alkali heating treated sample and the values were further reduced to about 10% of dopamine-modified sample and 7% of APTMS-modified sample. The hemolysis rate was below 5% for the 3-phosphonopropionic acid modified sample. As compared to the pristine magnesium alloy, fewer platelets were attached and activated on the modified surfaces and the activated partial thromboplastin times (APTT) were prolonged to some degree. Furthermore, the modified samples showed good cytocompatibility. Endothelial cells exhibited the improved proliferative profiles in terms of CCK-8 assay as compared to those on the pristine magnesium alloy. The modified samples showed better endothelial cell adhesion and spreading than the pristine magnesium alloy. Taking all these results into consideration, the method of this study can be used to modify the magnesium alloy surface to improve the corrosion resistance and biocompatibility simultaneously.


Assuntos
Ligas , Materiais Revestidos Biocompatíveis , Magnésio , Teste de Materiais , Compostos Organofosforados , Propilaminas , Silanos , Ligas/química , Ligas/farmacologia , Materiais Revestidos Biocompatíveis/química , Materiais Revestidos Biocompatíveis/farmacologia , Corrosão , Feminino , Humanos , Magnésio/química , Magnésio/farmacologia , Masculino , Compostos Organofosforados/química , Compostos Organofosforados/farmacologia , Propilaminas/química , Propilaminas/farmacologia , Silanos/química , Silanos/farmacologia
14.
Mater Sci Eng C Mater Biol Appl ; 63: 333-40, 2016 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-27040227

RESUMO

Owing to its unique physical and chemical properties, graphene oxide (GO) has attracted tremendous interest in many fields including biomaterials and biomedicine. The purpose of the present study is to investigate the endothelial cell behaviors and anticoagulation of heparin-loaded GO coating on the titanium surface. To this end, the titanium surface was firstly covered by the polydopamine coating followed by the deposition of the GO coating. Heparin was finally loaded on the GO coating to improve the blood compatibility. The results of attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR), Raman spectroscopy and X-ray photoelectron spectroscopy (XPS) indicated that the heparin-loaded GO coating was successfully created on the titanium surface. The scanning electron microscopy (SEM) images indicated that a relative uniform GO coating consisting of multilayer GO sheets was formed on the substrate. The hydrophilicity of the titanium surface was enhanced after the deposition of GO and further improved significantly by the loading heparin. The GO coating can enhance the endothelial cell adhesion and proliferation as compared with polydopamine coating and the blank titanium. Loading heparin on the GO coating can significantly reduce the platelet adhesion and prolong the activated partial thromboplastin time (APTT) while not influence the endothelial cell adhesion and proliferation. Therefore, the heparin-loaded GO coating can simultaneously enhance the cytocompatibility to endothelial cells and blood compatibility of biomaterials. Because the polydopamine coating can be easily prepared on most of biomaterials including polymer, ceramics and metal, thus the approach of the present study may open up a new window of promising an effective and efficient way to promote endothelialization and improve the blood compatibility of blood-contact biomedical devices such as intravascular stents.


Assuntos
Materiais Revestidos Biocompatíveis/química , Grafite/química , Heparina/química , Coagulação Sanguínea/efeitos dos fármacos , Adesão Celular/efeitos dos fármacos , Linhagem Celular , Materiais Revestidos Biocompatíveis/farmacologia , Células Endoteliais/citologia , Células Endoteliais/metabolismo , Heparina/farmacologia , Humanos , Indóis/química , Microscopia Eletrônica de Varredura , Microscopia de Fluorescência , Óxidos/química , Tempo de Tromboplastina Parcial , Espectroscopia Fotoeletrônica , Adesividade Plaquetária/efeitos dos fármacos , Polímeros/química , Análise Espectral Raman , Propriedades de Superfície , Titânio
15.
Biomed Mater Eng ; 24(1): 781-7, 2014.
Artigo em Inglês | MEDLINE | ID: mdl-24211964

RESUMO

This paper presents a simple method to sequentially immobilize poly (ethylene glycol) (PEG) and albumin on titanium surface to enhance the blood compatibility. Attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR) analysis indicated that PEG and albumin were successfully immobilized on the titanium surface. Water contact angle results showed a better hydrophilic surface after the immobilization. The immobilized PEG or albumin can not only obviously prevent platelet adhesion and activation but also prolong activated partial thromboplastin time (APTT), leading to the improved anticoagulation. Moreover, immobilization of albumin on PEG-modified surface can further improve the anticoagulation. The approach in the present study provides an effective and efficient method to improve the anticoagulation of blood-contact biomedical devices such as coronary stents.


Assuntos
Albuminas/química , Anticoagulantes/química , Coagulação Sanguínea/efeitos dos fármacos , Polietilenoglicóis/química , Titânio/química , Adsorção , Plaquetas/metabolismo , Materiais Revestidos Biocompatíveis , Humanos , Interações Hidrofóbicas e Hidrofílicas , Teste de Materiais , Microscopia Eletrônica de Varredura , Tempo de Tromboplastina Parcial , Adesividade Plaquetária , Espectroscopia de Infravermelho com Transformada de Fourier , Stents , Propriedades de Superfície , Temperatura Ambiente , Água/química
16.
Colloids Surf B Biointerfaces ; 112: 508-12, 2013 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-23972476

RESUMO

Titanium and its alloys have been widely used for blood-contacting biomedical devices; however, their blood compatibility needs to be improved. In this study, titanium surface was modified by sequential immobilization of oligo(ethylene glycol) (OEG) and 2-methacryloyloxyethyl phosphorylcholine (MPC) to improve its anticoagulation. Water contact angle results showed an excellent hydrophilic surface after the immobilization. Attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR) confirmed that OEG and MPC were successfully immobilized on titanium surface. Static platelet adhesion and APTT (activated partial thromboplastin time) experiments suggested that the anticoagulation of titanium was significantly enhanced by the immobilization of OEG and further by subsequent MPC grafting. The approach in the present study opens up a window of promising an effective and efficient method to improve the anticoagulation of blood-contact biomedical devices such as coronary stents.


Assuntos
Anticoagulantes/química , Anticoagulantes/farmacologia , Metacrilatos/química , Fosforilcolina/análogos & derivados , Polietilenoglicóis/química , Titânio/química , Titânio/farmacologia , Materiais Revestidos Biocompatíveis/química , Materiais Revestidos Biocompatíveis/farmacologia , Stents Farmacológicos , Humanos , Interações Hidrofóbicas e Hidrofílicas , Teste de Materiais , Tempo de Tromboplastina Parcial , Fosforilcolina/química , Adesividade Plaquetária/efeitos dos fármacos , Espectroscopia de Infravermelho com Transformada de Fourier , Propriedades de Superfície , Trombose/prevenção & controle
17.
Colloids Surf B Biointerfaces ; 104: 18-26, 2013 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-23298583

RESUMO

In this study, we report a simple method for creating extracellular matrix (ECM) protein patterns to control osteoblast cell adhesion and spreading. The fibronectin patterns are directly produced on polystyrene (PS) surfaces by microcontact printing (µCP). Confocal laser scanning microscopy (CLSM) images show that protein patterns are successfully fabricated on PS surfaces. Newborn rat osteoblast cells are then seeded on these protein patterns and cultured for 4 days. The results demonstrate that osteoblast cells preferentially adhere and grow on the protein areas. The pattern dimensions have significant influences on cell behaviors, including cell adhesion, spreading, distribution, and growth direction. Therefore, it is possible to control the cell morphology and even cell function by carefully designing the pattern shapes and sizes. The present study suggests that the ECM protein patterns can be used to modify biomaterials' surfaces and spatially control the morphologies of osteoblast cells. We believe that our work could find applications for creating patterned bioactive surfaces to control cell adhesion, spreading and cell function. It may be helpful for the development of novel implantable biomaterials, such as artificial bone implants, where control of interfacial biological interactions between implants and cells would be preferable.


Assuntos
Fibronectinas/química , Osteoblastos/citologia , Animais , Adesão Celular , Movimento Celular , Células Cultivadas , Tamanho da Partícula , Poliestirenos/química , Impressão , Ratos , Propriedades de Superfície
18.
Sheng Wu Yi Xue Gong Cheng Xue Za Zhi ; 30(5): 1044-51, 2013 Oct.
Artigo em Chinês | MEDLINE | ID: mdl-24459968

RESUMO

The main purpose of the present study was to investigate the cell behaviors of rat primary osteoblast cells on extracellular matrix (ECM) protein micropatterns. For this purpose, a series of fibronectin micropatterns with different shapes and varying dimensions were created on polystyrene (PS) surfaces by microcontact printing. The results of confocal laser scanning microscopy (CLSM) images indicated that excellent micropatterns were successfully obtained. These protein patterns were stable during the cell culture. The cell experiments suggested that the osteoblast cells preferentially attached onto protein-functionalized areas and displayed different cell shape and spreading behavior on different protein micropatterns. The protein micropatterns can significantly influence the cell adhesion, spreading, alignment and orientation and so on. Therefore, this work can be used to modify biomaterial surfaces, especially that of bone-implant biomaterials, to effectively control cell behavior. It further contributes to clarify the interfacial biological behaviors between biomaterials and osteoblast and can provide the cues for development of bone implantable materials which is able to modulate osteoblast cell growth behavior.


Assuntos
Adesão Celular/efeitos dos fármacos , Movimento Celular/efeitos dos fármacos , Proteínas da Matriz Extracelular/farmacologia , Osteoblastos/citologia , Animais , Animais Recém-Nascidos , Células Cultivadas , Ratos , Propriedades de Superfície
19.
Colloids Surf B Biointerfaces ; 102: 730-6, 2013 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-23107951

RESUMO

The main purpose of the present study is to investigate the influences of collagen VI (col-VI) patterns on human chondrocytes behaviors. To this end, col-VI stripes with varying width and interstripe spacing are created on polystyrene (PS) surfaces by microcontact printing (µCP). Human chondrocytes are then seeded on these protein patterns and the cell adhesion and alignment are investigated by staining the vimentin and matrilin-3 secreted by seeded chondrocytes. The results indicate that the cells preferentially attach onto the protein areas, rendering cell patterns and the elongated cell shapes. The pattern dimensions can significantly influence cell adhesion, spreading and orientation. The stripe protein patterns can guide cell adhesion and alignment. The cell morphologies can be controlled by carefully designing the pattern shapes and sizes. Our results suggest that the protein patterns can be used to modify biomaterials' surfaces for selective cell-binding and cell alignment. It could provide some cues for the development of novel implantable biomaterials, such as tissue-engineered scaffolds for cartilage replacement, where specific cell alignment is needed.


Assuntos
Adesão Celular/efeitos dos fármacos , Condrócitos/citologia , Condrócitos/efeitos dos fármacos , Proteínas da Matriz Extracelular/química , Proteínas da Matriz Extracelular/farmacologia , Vimentina/metabolismo , Condrócitos/metabolismo , Colágeno Tipo VI/química , Colágeno Tipo VI/farmacologia , Proteínas da Matriz Extracelular/metabolismo , Humanos , Proteínas Matrilinas , Engenharia Tecidual/métodos
20.
J Mater Sci Mater Med ; 22(4): 989-95, 2011 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-21424212

RESUMO

The aim of the study was to explore the feasibility of the Ca-P coating titanium alloy plate to be used as the vancomycin drug-delivery system by biomimetic coating technology. Through the X-ray diffraction study, the main components of the coatings were identified as octocalcium phosphate. The in vitro vancomycin release, bacteriostasis activity to Staphylococcus aureus (S. aureus), the scanning electron microscope (SEM) image and osteoblast adhesion and proliferation test of vancomycin-loaded Ca-P coating plate were evaluated. The bacteriostatic activity of the vancomycin-loaded Ca-P coating plate showed a continuous drug release and had an inhibitory effect on the growth of the S. aureus. In vitro osteoblast culture results showed that the Ca-P coating plate loaded with or without the vancomycin both obviously promoted the osteoblast attachment. It was suggested that the vancomycin-loaded Ca-P coating may be compounded in the surface of the internal fixators to reduce the incidence of the implant-associated infection.


Assuntos
Ligas/química , Antibacterianos/farmacologia , Titânio/química , Vancomicina/farmacologia , Animais , Aderência Bacteriana , Fosfatos de Cálcio/química , Proliferação de Células , Sistemas de Liberação de Medicamentos , Técnicas In Vitro , Microscopia Eletrônica de Varredura/métodos , Modelos Estatísticos , Osteoblastos/citologia , Osteoblastos/efeitos dos fármacos , Ratos , Staphylococcus aureus/metabolismo , Vancomicina/análogos & derivados , Vancomicina/química
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA