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1.
Zhonghua Yu Fang Yi Xue Za Zhi ; 55(9): 1139-1144, 2021 Sep 06.
Artigo em Chinês | MEDLINE | ID: mdl-34619934

RESUMO

The liquid chromatography tandem mass spectrometry was used to detect the urinary proteomics of 223 residents aged 40-69 years old who participated in the National Upper Gastrointestinal Cancer Early Detection Program in Linqu County, Shandong Province from November 22 to December 7, 2018, and analyze the alcohol consumption related proteomic profiles and individual urinary protein. There were significant differences in urinary protein profiles between alcohol consumption group and non-alcohol consumption group. The expression of 26 urinary proteins was up-regulated and 20 urinary proteins were down-regulated in alcohol consumption group (P<0.05). The differentially expressed proteins had enzyme inhibitor activity and phospholipid binding function, and mainly enriched in pathways involving proximal tubule bicarbonate regeneration, complement and coagulation cascade, and cholesterol metabolism. The protein expressions of complement factor I (CFI), angiotensin converting enzyme 2 (ACE2) and protein C inhibitor (SERPINA5) were positively correlated with daily alcohol consumption.


Assuntos
Neoplasias Gastrointestinais , Proteômica , Adulto , Idoso , Consumo de Bebidas Alcoólicas , Cromatografia Líquida , Detecção Precoce de Câncer , Humanos , Pessoa de Meia-Idade
2.
Zhonghua Yu Fang Yi Xue Za Zhi ; 55(1): 104-109, 2021 Jan 06.
Artigo em Chinês | MEDLINE | ID: mdl-33455140

RESUMO

Traditional survival methods have a wide application in the field of biomedical research. However, applying traditional survival methods requires data to meet a set of special assumptions while the Random Survival Forest model can overcome this inconvenience. Herein, we used the clinical data of Primary Biliary Cholangitis (PBC) from Mayo Clinic to introduce and demonstrate Random Survival Forest model from mathematical principles, model building, practical example and attentions, aiming to provide a novel method for doing survival analysis.


Assuntos
Algoritmos , Aprendizado de Máquina , Humanos , Análise de Sobrevida
3.
Zhonghua Liu Xing Bing Xue Za Zhi ; 40(12): 1527-1532, 2019 Dec 10.
Artigo em Chinês | MEDLINE | ID: mdl-32062910

RESUMO

Objective: To analyze the association between circular RNAs expression in serum and gastric cancer and evaluate the potential of the related markers in early diagnosis of gastric cancer. Methods: Forty eight gastric cancer cases in Linqu County People's Hospital were selected as case group, and 48 controls matched by age and sex were randomly selected in the gastric cancer screening cohort during the same period. The expression levels of hsa_circ_002059, hsa_circ_0000096 and hsa_circ_0001895 were detected by quantitative real-time PCR. The results were compared between case group and control group. Results: The positive expression rates of hsa_circ_002059, hsa_circ_0000096 and hsa_circ_0001895 were 70.8%, 47.9%, 75.0% in case group, slightly higher than those in control group (58.3%, 31.3%, 60.4%), although P values were all more than 0.05. The expression level medians of the 3 candidate circRNAs expression levels were 1.60% (0-5.64%), 0 (0-0.61%), 0.91% (0.06%-1.88%) in case group, while 0.05% (0-6.07%), 0 (0-0.34%), 0.42% (0-1.39%) in control group, respectively. Conditional logistic regression analysis showed that the association strength of high expressions of 3 candidate circRNAs with gastric cancer showed an increase trend, but the differences had no significance after adjusted by Helicobacter pylori infection, smoking and drinking status (all P>0.05). Further analysis by combining the 3 candidate circRNAs showed the increased strength of association between circRNAs and gastric cancer with the elevated number of positive circRNAs in serum (trend test P=0.040) compared with circRNAs negative persons. Conclusion: Our study preliminarily suggested that the expression of hsa_circ_002059, hsa_circ_0000096 and hsa_circ_0001895 in serum might be correlated with gastric cancer.


Assuntos
Infecções por Helicobacter , RNA Circular , Neoplasias Gástricas , Helicobacter pylori , Humanos , RNA , RNA Circular/sangue , RNA Circular/metabolismo , Fumar , Neoplasias Gástricas/sangue , Neoplasias Gástricas/metabolismo
4.
Genet Mol Res ; 13(4): 8596-608, 2014 Jan 24.
Artigo em Inglês | MEDLINE | ID: mdl-24615086

RESUMO

Apnea and the respiratory cycle are dynamic processes in obstructive sleep apnea-hypopnea (OSAH), which occur only during sleep. Our study aimed to observe the dynamic changes in the soft palate and the uvula during wakefulness and sleep using ultrafast magnetic resonance imaging (UMRI) to provide reference data for the pathogenesis and treatment of OSAH. The dynamic changes in the soft palate and uvular tip of 15 male patients (average age: 50.43 ± 9.82 years) with OSAH were evaluated using UMRI of the upper airway while asleep and awake after 1 night of sleep deprivation. A series of midline sagittal images of the upper airway were obtained. The distance from the center of the soft palate to the x-axis (an extended line from the anterior nasal spine to the posterior nasal spine), from the uvular tip to the x-axis, from the center of the soft palate to the y-axis (a perpendicular line from the center of the pituitary to the x-axis), and from the uvular tip to the y-axis (designated as PX, UX, PY, and UY, respectively) were measured during sleep and wakefulness. The minimum PX, PY, UX, and UY were shorter during sleep than during wakefulness, whereas the maxima were longer during sleep (P < 0.01), the differences between the maximum and minimum PX, PY, UX, and UY were larger during sleep (P < 0.01). The upward, downward, forward, and backward ranges of movement of the soft palate and the uvular tip were larger during sleep in OSAH patients. This increased compliance may trigger each airway obstructive event.


Assuntos
Apneia Obstrutiva do Sono/fisiopatologia , Úvula/fisiopatologia , Adulto , Humanos , Imageamento por Ressonância Magnética , Masculino , Pessoa de Meia-Idade , Movimento , Apneia Obstrutiva do Sono/diagnóstico
5.
Int J Gynecol Cancer ; 16 Suppl 1: 172-8, 2006.
Artigo em Inglês | MEDLINE | ID: mdl-16515586

RESUMO

The role of germline BRCA1 mutations in hereditary breast and ovarian cancer (HBOC) has been well established in women in Western countries. However, relatively few studies have been carried out in Chinese population. In the present study, we investigated the frequency and spectrum of germline BRCA1 mutations in Chinese HBOC patients, all of whom were from northern part of China. A total of 25 women with HBOC and ten relatives were analyzed. Mutation screening was performed by a combination of denaturing high-performance liquid chromatography and sequencing. Seven protein-truncating mutations were identified. They were 667delG, 3347A --> T, 3478del5, 4255delCT, 1235A --> G, 2064G --> T, and 5589del8. The first four of the mutations were putative ones never reported before. The prevalence of the protein-truncating mutations in this HBOC series was 40.0%, which is similar to that observed in Western hereditary ovarian cancer patients but higher than that reported in Chinese women with sporadic breast and ovarian cancer. Among the ten relatives we analyzed, six shared the same mutations with their affected relatives. No ovarian cancer was detected after 19 months of follow-up. This study showed that BRCA1 mutations play an important role in Northern Chinese HBOC.


Assuntos
Neoplasias da Mama/genética , Genes BRCA1 , Síndromes Neoplásicas Hereditárias/genética , Neoplasias Ovarianas/genética , Adulto , Idoso , Grupo com Ancestrais do Continente Asiático/genética , Feminino , Predisposição Genética para Doença , Técnicas Genéticas , Mutação em Linhagem Germinativa/genética , Heterozigoto , Humanos , Pessoa de Meia-Idade
6.
Ann Epidemiol ; 11(8): 543-6, 2001 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-11709273

RESUMO

BACKGROUND: Studies in adult populations in selected countries with widely varying rates of gastric cancer have shown a weak correlation between gastric cancer mortality rates and the prevalence of CagA+ strains of H. pylori. However, only limited data are available in ethnically homogenous populations with varying rates in the same region. METHODS; We compared the prevalence of H. pylori in general and of CagA+ strains in particular among children in Shandong Province, China in areas at high (Linqu County) and low risk (Cangshan County) of gastric cancer. H. pylori status among children aged 3 to 12 years was determined by 13C-UBT, and CagA status was determined by enzyme-linked immunosorbent assay (ELISA). Because of the difficulty in obtaining blood from young children aged 3 to 4 years and from some children aged 5 years, CagA status was determined among part of children 5 years old and children 6 to 12 years old. RESULTS; Among 98 children aged 3 to 12 years in Linqu, 68 (69.4%) was H. pylori-positive, as compared with 29 (28.7%) among 101 children in Cangshan. Among children positive for 13C-UBT, the proportion of the CagA+ strains were identified was 46 (88.5%) of 52 in Linqu and 13 (81.3%) of 16 in Cangshan, respectively. CONCLUSIONS: The prevalence of H. pylori was nearly three times higher among children in Linqu than in Cangshan, which may contribute to the large differential in gastric cancer rates for two neighboring populations in Shandong Province.


Assuntos
Antígenos de Bactérias/sangue , Proteínas de Bactérias/análise , Infecções por Helicobacter/diagnóstico , Helicobacter pylori/imunologia , Helicobacter pylori/patogenicidade , Neoplasias Gástricas/microbiologia , Anticorpos Antibacterianos/sangue , Testes Respiratórios/métodos , Isótopos de Carbono , Criança , Pré-Escolar , China/epidemiologia , Ensaio de Imunoadsorção Enzimática , Feminino , Infecções por Helicobacter/epidemiologia , Infecções por Helicobacter/imunologia , Humanos , Masculino , Prevalência , Risco , Neoplasias Gástricas/diagnóstico , Neoplasias Gástricas/imunologia , Ureia/análise
7.
Shanghai Kou Qiang Yi Xue ; 10(3): 233-5, 2001 Sep.
Artigo em Chinês | MEDLINE | ID: mdl-14994002

RESUMO

OBJECTIVE: To study the effects of artificial bone composite of basic fibroblast growth factor(bFGF) with natural non organic bone(NNB)in repairing mandibular defects in rabbits,in order to provide an ideal bone replacement material for clinic. METHODS: 15mm x 6mm bilateral mandibular periosteum bone defects were made surgically in 30 New Zealand adult rabbits and each defect as an experimental unit. The defects were randomly repaired with composite bone, NNB,fresh autogenous bone at 3,6,12 weeks after surgery according to the total random design, blank control group was stayed. Specimens obtained were evaluated with general observation, X-ray as well as computerized histological microscopic analysis. RESULTS: New bone formed and calcified earlier in composite bone and the bone area was greater in the same period than that in NNB. The new bone area in composite bone was still smaller than that of autogenous bone at 6 weeks, while at 12 weeks they had no significant differences. The blank defects were not repaired even in the end. CONCLUSION: Composite bone had sound biocompatibility. It was much better than NNB and can be compared with autogenous bone 12 weeks after operation. So it can be an ideal alternative for bone replacement.

8.
Shanghai Kou Qiang Yi Xue ; 10(2): 128-31, 2001 Jun.
Artigo em Chinês | MEDLINE | ID: mdl-14994037

RESUMO

OBJECTIVE: To study the conditions of healing of mature tooth in dogs after long cryopreservation followed by replantation. METHODS: The tooth was extracted after filling the root canals and then preserved for one week and three months respectively by Schwartz's method of cryopreservation of periodontal membrane. The specimens were took out eight weeks after replantation. It was demineralized in a 5% solution of formic acid,and then sectioned perpendicular to the long axes of tooth at a thickness of 5microm. The tooth were sectioned in step-serial sections at 500 microm intervals. The sections were stained with hematoxylin-eosin and followed by evaluation according to Andreasen. RESULTS: The percentage of healing of periodontal membrane (PHPM) of the group of cryopreservation for one week was 64.53%. It was lower than the PHPM of the group of immediate replantation (85%), but the difference demonstrated no significance (P>0.05). The PHPM of the group of cryopreservation for three months was 50.3%. It was lower than the PHPM of the group of immediate replantation significantly (P<0.05). CONCLUSION: The human's periodontal membrane may be cryopreserved longly and heals after the replantation of tooth as the human's tissues of corneas, skin, islets, et al may be done. The alveolar cavities to be newly made affect the healing of periodontal membrane in some degree.

9.
Shanghai Kou Qiang Yi Xue ; 4(2): 66-7, 1995 Jun.
Artigo em Chinês | MEDLINE | ID: mdl-16538326

RESUMO

The study of teeth,dentition and occlusion is helpful to guide oral medical clinic and the study of anthropology.Our department of Osaka Dental University and had many items of research work about teeth,dentition and occlusion between Chinese and Japanese students.This article reports the study of Carabelli cusp.The results revealed:(1)the rate of Carabelli cusp is higher in Japanese than in Chinese and is higher in male than in female.(2)the rate of Carabelli cusp of the first maxillary molar of both side is very high.(3)The rate of Carabelli cusp is related to the height and the type of cusp numbers.These all illustrated the occurence of Carabeiil cusp were influenced by race, individual and the development of teeth.

11.
Shanghai Kou Qiang Yi Xue ; 2(4): 212-3, 1993 Dec.
Artigo em Chinês | MEDLINE | ID: mdl-15159807

RESUMO

37 cases of mandible of Neolithic adult that both sexes without missing teeth,alveolar bone were studied.The result showed that the location of the mental foramen below the apex of the lower second premolar was the most common(67.97%) in the modern adult,and the location of the mental foramen below between the apex of the lower second premolar and the first molar was the most common (64.86%) in the neolithic adult.

13.
Shanghai Kou Qiang Yi Xue ; 2(1): 40-1, 1993 Mar.
Artigo em Chinês | MEDLINE | ID: mdl-15159882
14.
J Reconstr Microsurg ; 1(1): 75-80, 1984 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-6544346

RESUMO

The authors discuss indications for the use of microvascular free flaps transferred to the oral and maxillofacial region. A series of 139 cases, involving 145 free flaps, is reported over a three year period. The overall success rate for all types of flaps was 89.3%. Advantages and disadvantages of the use of various flaps are presented and suggestions are offered to avoid complications.


Assuntos
Neoplasias Faciais/cirurgia , Neoplasias Maxilares/cirurgia , Microcirurgia/métodos , Neoplasias Bucais/cirurgia , Retalhos Cirúrgicos , Procedimentos Cirúrgicos Vasculares/métodos , Adolescente , Adulto , Idoso , Feminino , Humanos , Masculino , Pessoa de Meia-Idade
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