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J Mater Chem B ; 8(45): 10346-10352, 2020 Dec 07.
Artigo em Inglês | MEDLINE | ID: mdl-32657318


One-dimensional (1D) morphology-unique Au-Ag2S nano-hybrids are achieved by combining the interfacial self-assembly of Ag nanowires, interface-oriented site-specific etching of Ag nanowires with AuCl4-, and the sulfurization of S2-. The as-formed Au-Ag2S nano-hybrid has a trough-like morphology. The wall of the Au-Ag2S nanotrough is a Ag2S/Au/Ag2S trilayer wall, but the Ag2S layer is a Ag2S-rich mixture of Ag2S and Au rather than pure Ag2S because of the diffusion of Au atoms towards Ag2S. The Au-Ag2S nanotrough shows strong absorption in the visible region (400-800 nm) and exhibits a favorable photoelectrochemical (PEC) response, the photocurrent of which is ∼8.5 times larger than that of pure Ag2S. This enhanced PEC response originates from the localized plasmonic resonance effect of Au. Moreover, the PEC biosensor based on the Au-Ag2S nanotroughs shows high sensitivity and selectivity, satisfactory reproducibility, and good stability towards human α-thrombin (TB) detection: a sensitive linear response ranging from 1.00 to 10.00 pmol L-1 and a low detection limit of 0.67 pmol L-1. This study provides a new model for studying the PEC behavior of plasmonic metal/semiconductor materials, and this Au-Ag2S nanotrough may also be useful in the fields of photocatalysis and photovoltaics.

Chem Asian J ; 15(17): 2742-2748, 2020 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-32658379


Although linker-free Au nanoparticle superstructures (AuNPSTs) have demonstrated to have satisfactory photothermal conversion efficiency owing to their enhanced visible-near-infrared absorption caused by the interparticle coupling, they cannot be used directly for in vivo photothermal therapy (PTT) of cancer because of poor stability. To address this issue, we herein propose a polymer-coating strategy, dressing AuNPST on a poly(dopamine) (PDA) coat, and successfully investigate the in vivo PTT effect of AuNPSTs. By employing Triton X-100 as an emulsifier for the formation of AuNPSTs, dopamine was site-specifically polymerized around each AuNPST by the interaction between -OH of Triton X-100 and -NH2 of dopamine. As-fabricated AuNPST/PDA has a sphere-like shape with an average diameter of ∼106 nm and the PDA shell is about 10 nm PDA thick. The AuNPST/PDA shows enhanced durability to heat, acid, and alkali compared with bare AuNPST. Also, under 808 nm laser irradiation, AuNPST/PDA shows photothermal conversion efficiency of ∼33%, higher than bare AuNPST (∼23%). Significantly, AuNPST/PDA can be used as in-vitro and in-vivo PTT agent and shows excellent therapeutic efficacy for tumor ablation thanks to its enhanced stability and biocompatibility, indicative of its potential practicability in clinical PTT.

Cytotherapy ; 18(2): 186-97, 2016 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-26596504


The prevalence of end-stage renal disease is emerging as a serious worldwide public health problem because of the shortage of donor organs and the need to take lifelong immunosuppressive medication in patients who receive a transplanted kidney. Recently, tissue bioengineering of decellularization and recellularization scaffolds has emerged as a novel strategy for organ regeneration, and we review the critical technologies supporting these methods. We present a summary of factors associated with experimental protocols that may shed light on the future development of kidney bioengineering and we discuss the cell sources and bioreactor techniques applied to the recellularization process. Finally, we review some artificial renal engineering technologies and their future prospects, such as kidney on a chip and the application of three-dimensional and four-dimensional printing in kidney tissue engineering.

Regeneração Tecidual Guiada/métodos , Falência Renal Crônica/terapia , Regeneração , Medicina Regenerativa/tendências , Engenharia Tecidual/métodos , Animais , Reatores Biológicos , Humanos , Células-Tronco Pluripotentes Induzidas/citologia , Rim/citologia , Rim/patologia , Organogênese , Ratos , Tecidos Suporte
Expert Rev Gastroenterol Hepatol ; 9(9): 1183-91, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-26220044


Techniques for producing decellularized scaffolds for use in liver tissue engineering are emerging as promising methods for tissue reconstruction. In this article, the authors present an overview of liver decellularization methods developed and applied in recent years. These include the widespread use of various perfusion methods for the generation of a 3D scaffold, which may function as a template for either cell recellularization or direct biological application. The authors evaluate methods for scaffold production and explore some factors that may affect the decellularization process. In addition to tissue engineering, this overview includes a description of other potential applications for a decellularized liver scaffold. The authors also introduce the concept of fabrication of fragile biomaterial architecture and finally review the cell types applied to liver scaffold engineering.

Matriz Extracelular , Fígado , Engenharia Tecidual/métodos , Tecidos Suporte , Materiais Biocompatíveis , Humanos , Fígado/citologia , Fígado/fisiologia , Perfusão/métodos
Cytotherapy ; 17(8): 1015-24, 2015 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-25981396


Allogeneic transplantation is the definitive treatment for patients with end-stage liver disease but is limited by donor shortage and very high cost. Through de-cellularization and re-cellularization methods, re-engineered liver may provide a promising alternative for treating patients with end-stage liver disease. To achieve this, the prevention of the native extracellular matrix ultrastructure plays a central role in de-cellularization protocol; the re-seeding cell types, as well as re-seeding strategies, need more explorations in re-cellularization protocol. Some success of this approach has been published in a rat model; however, the re-engineered liver remains functional in vivo for only several hours, which suggests that the recent protocol may be far from the ideal target. This Review highlights the challenges still to be overcome and presents an overview and summary of methods of de-cellularization and re-cellularization strategies, together with a view on future directions that may lead to the regeneration of a functional liver.

Doença Hepática Terminal/cirurgia , Hepatócitos/transplante , Regeneração Hepática/fisiologia , Transplante de Fígado/métodos , Fígado/citologia , Engenharia Tecidual/tendências , Animais , Matriz Extracelular/metabolismo , Humanos , Ratos , Doadores de Tecidos , Transplante Homólogo
J Cell Biochem ; 115(7): 1269-76, 2014 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-24464651


The aberrant expression of microRNAs (miRNAs) has been found in various types of cancer. The present study found miR-20a was significantly up-regulated in prostate cancer compared with normal prostate tissues. Patients with a higher miR-20a expression had a Gleason score of 7-10 and shorter survival time. The transwell and wound healing assays revealed that blocking expression of miR-20a by miR-20a ASO suppresses the invasion and migration of PC-3 and DU145 cells in vitro and also inhibits tumor growth in vivo. Furthermore, we identified miR-20a directly targets the ABL family non-receptor tyrosine kinases ABL2 and negatively regulates the phosphorylation of its downstream gene p190RhoGAP. Knockdown of ABL2 promoted cell invasion and migration and we identified miR-20a-induced cell invasion and migration can be rescued by ABL2. In conclusion, our findings show that miR-20a significantly contributes to the progression of prostate cancer by targeting ABL2.

Movimento Celular/genética , MicroRNAs/genética , Invasividade Neoplásica/genética , Neoplasias da Próstata/patologia , Proteínas Tirosina Quinases/genética , Regiões 3' não Traduzidas/genética , Linhagem Celular Tumoral , Proliferação de Células , Proteínas Ativadoras de GTPase/metabolismo , Regulação Neoplásica da Expressão Gênica , Humanos , Masculino , Gradação de Tumores , Fosforilação , Próstata/citologia , Neoplasias da Próstata/genética , Neoplasias da Próstata/mortalidade , Proteínas Tirosina Quinases/biossíntese , Proteínas Proto-Oncogênicas c-abl/biossíntese , Proteínas Proto-Oncogênicas c-abl/genética , Interferência de RNA , RNA Mensageiro/genética , RNA Interferente Pequeno , Regulação para Cima