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1.
Holist Nurs Pract ; 36(1): 37-45, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-34783699

RESUMO

This qualitative study describes the psychological experience of patients hospitalized with COVID-19. These patients went through 3 psychological stages: extremely uncertainties during the initial diagnostic stage, complicated feelings of negativity during the treatment stage, and positive growth in the recovery stage. It is important for nurses to provide holistic care.


Assuntos
COVID-19 , Emoções , Humanos , Pesquisa Qualitativa , SARS-CoV-2
2.
Org Lett ; 2021 Nov 24.
Artigo em Inglês | MEDLINE | ID: mdl-34816718

RESUMO

Euphohyrisnoids A (1) and B (2), two highly rearranged lathyrane diterpenoids featuring a unique tetracyclo[10.2.2.01,10.03,7]cetane and tricyclo[8.4.1.03,7]pentadecane skeleton, respectively, were isolated from the seeds of Euphorbia lathyris. Their structures were determined by detailed spectroscopic analysis and were further confirmed by single-crystal X-ray diffraction. 1 significantly inhibited adipogenesis in 3T3-L1 adipocytes by retarding cell differentiation at the early stage.

3.
Appl Opt ; 60(32): 10186-10190, 2021 Nov 10.
Artigo em Inglês | MEDLINE | ID: mdl-34807125

RESUMO

We demonstrate a scheme for fabricating compact fiber Mach-Zehnder interferometer (MZI). A section of Ge-doped fiber (GDF) is sandwiched between two single-mode fibers. The sandwich structure is side polished to make the core of GDF exposed to the surroundings. Alkali solution is utilized to etch the core of GDF. A compact fiber MZI is achieved when about half of the core is etched. Compared with the traditional ones, our scheme for fabricating fiber MZI has the characteristics of low cost, environmentally friendly, and regular transmission spectrum. This fiber MZI not only reduces the consumption of the sample, but also brings forth a good potential for micro-scale detection of refractive index.

4.
Biochem Biophys Res Commun ; 583: 128-134, 2021 Oct 30.
Artigo em Inglês | MEDLINE | ID: mdl-34735874

RESUMO

Preeclampsia (PE) threatens the safety of mothers and fetuses, and its pathogenesis is still unclear. Our previous study has found the relationship between PE and serum Clusterin (CLU). This study aimed to investigate the role of CLU on PE. Firstly, levels of CLU in serum and placental tissue from PE patients and healthy pregnancies were compared. Then, RNA sequencing, cell counting kit-8, matrigel invasion, cell apoptosis, and angiogenesis assay were performed to evaluate the role of CLU on primary isolation trophoblast cells. We found the expression of CLU was increased before the clinical syndrome occurred, whereas its level was positively related to the severity of PE. CLU significantly inhibited the expression of matrix metalloproteinase-9 and Vimentin and enhanced E-cadherin to inhibit epithelial-mesenchymal transition of trophoblast cells, further reducing its migration and invasion. Our results suggested that CLU may play a role in regulating trophoblast invasion and migration during placental development, which may be one of the risk factors for PE.

5.
Atherosclerosis ; 337: 35-41, 2021 Oct 18.
Artigo em Inglês | MEDLINE | ID: mdl-34757269

RESUMO

BACKGROUND AND AIMS: Elevated fibrinogen levels have been observed in patients with acute ischemic stroke, but the association of fibrinogen with stroke outcomes is still undefined. We aimed to assess the association between baseline or 90-day fibrinogen levels and long-term outcomes in patients with ischemic stroke or transient ischemic attack (TIA). METHODS: Using data from the China National Stroke Registry Ⅲ, this substudy included 10 518 patients within 7 days (baseline) of onset and 6268 patients at 90 days of recovery. Multivariate Cox regression and logistic regression analyses were used to assess the associations of fibrinogen with poor functional outcome (modified Rankin Scale score 3-6), dependence (modified Rankin Scale score 3-5), all-cause death, and stroke recurrence at 1 year. RESULTS: Fibrinogen levels at 90 days were higher than those at baseline (443.5 mg/dl versus 393.7 mg/dl; p < 0.001). A high baseline fibrinogen level was associated with poor functional outcome (odds ratio [OR], 1.63; 95% confidence interval [CI], 1.35-1.97) and dependence (OR, 1.68; 95% CI, 1.36-2.09) after adjusting for all confounding risk factors. In contrast, further adjustment for high-sensitivity C-reactive protein attenuated the association between baseline fibrinogen level and all-cause death or stroke recurrence. Furthermore, a high 90-day fibrinogen level was also associated with poor functional outcome (OR, 1.46; 95% CI, 1.07-2.00) and dependence (OR, 1.43; 95% CI, 1.03-1.98) after adjusting for all confounding risk factors. CONCLUSIONS: High baseline and 90-day fibrinogen levels were associated with outcomes in patients with ischemic stroke or TIA.

6.
Front Surg ; 8: 690803, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34604292

RESUMO

Background: In the perioperative management of Total Knee Arthroplasty (TKA), postoperative fever has always been a concern. Current research focuses on infectious fever, and there is no relevant research on the occurrence of non-infectious fever (NIF) and its risk factors. Hence, the aim of this study was to clarify the risk factors for NIF after TKA, and construct an easy-to-use nomogram. Methods: A retrospective cohort study was conducted. Consecutive patients undergoing primary unilateral TKA were divided into the non-infectious fever group and the control group. Clinicopathological characters were collected from electronic medical records. Univariate Logistic regression was used to analyze the related independent risk factors. The optimal threshold for each selected factor and combined index was determined when the Youden index achieved the highest value. And the predictive nomogram was developed by these independent factors. Results: Ultimately, 146 patients were included in this study. Of them, 57 (39.04%) patients experienced NIF. Results of the univariable logistic regression analysis indicated that intraoperative blood loss (OR, 1.002; 95% CI, 1.000-1.0004), postoperative drainage fluid volume (OR, 1.003; 95% CI, 1.001-1.006) and frequency of blood transfusion (n = 1; OR, 0.227; 95% CI, 0.068-0.757) were independent risk factors of NIF occurrence. The predictive nomogram that incorporated the above independent risk factors was developed, and it yielded an areas under the curves (AUC) of 0.731 (95% CI: 0.651-0.801; P < 0.0001) with 54.39% sensitivity and 82.02% specificity. Conclusions: Non-infectious fever after TKA prolongs the time of antibiotic use and hospital stay. Our results demonstrated that the nomogram may facilitate to predict the individualized risk of NIF occurrence within 7-day by intraoperative blood loss, postoperative drainage fluid volume and frequency of blood transfusion.

7.
Front Oncol ; 11: 713530, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34660278

RESUMO

Anaplastic lymphoma kinase (ALK) is a validated molecular target for non-small-cell lung cancer (NSCLC). The use of tyrosine kinase inhibitors (TKIs) has led to significantly improved survival benefits. However, the clinical benefits of targeting ALK using TKIs are limited due to the emergence of drug resistance. The landscape of resistance mechanisms and treatment decisions has become increasingly complex. Therefore, continued research into new drugs and combinatorial therapies is required to improve outcomes in NSCLC. In this review, we explore the resistance mechanisms of ALK TKIs in advanced NSCLC in order to provide a theoretical basis and research ideas for solving the problem of ALK drug resistance.

8.
Brain Sci ; 11(10)2021 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-34679374

RESUMO

Every day, multilinguals around the world make important healthcare decisions while using a foreign language. The present study examined how the use of a native vs. non-native language shapes evaluations and decisions about preventative care. Bilinguals were randomly assigned to evaluate a series of medical scenarios in either their native or non-native language. Each scenario described potential adverse effects of a medical condition and a preventative treatment, as well as the population risk of disease- or treatment-related complications. Participants judged the perceived negativity and likelihood of experiencing adverse effects and indicated how willing they would be to accept the preventative treatment. We found that bilinguals using a foreign language perceived disease symptoms and treatment side effects to be less negative than those using their native tongue. Foreign language users were also more likely to account for the objective risks associated with medical conditions and treatments when making decisions about preventative care. We conclude that the use of a native vs. foreign language changes how people evaluate the consequences of accepting and declining preventative treatment, with potential implications for millions of providers and patients who routinely make medical choices in their non-native tongue.

9.
Front Chem ; 9: 756993, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34646815

RESUMO

On the origin of photoluminescence of noble metal NCs, there are always hot debates: metal-centered quantum-size confinement effect VS ligand-centered surface state mechanism. Herein, we provided solid evidence that structural water molecules (SWs) confined in the nanocavity formed by surface-protective-ligand packing on the metal NCs are the real luminescent emitters of Au-Ag bimetal NCs. The Ag cation mediated Au-Ag bimetal NCs exhibit the unique pH-dependent dual-emission characteristic with larger Stokes shift up to 200 nm, which could be used as potential ratiometric nanosensors for pH detection. Our results provide a completely new insight on the understanding of the origin of photoluminescence of metal NCs, which elucidates the abnormal PL emission phenomena, including solvent effect, pH-dependent behavior, surface ligand effect, multiple emitter centers, and large-Stoke's shift.

10.
Chem Commun (Camb) ; 57(70): 8826-8827, 2021 Sep 11.
Artigo em Inglês | MEDLINE | ID: mdl-34612294

RESUMO

Correction for 'High-efficiency methanol oxidation electrocatalysts realized by ultrathin PtRuM-O (M = Ni, Fe, Co) nanosheets' by Yue Pan et al., Chem. Commun., 2020, 56, 9028-9031, DOI: .

11.
Chemphyschem ; 2021 Oct 13.
Artigo em Inglês | MEDLINE | ID: mdl-34643984

RESUMO

Laser induced thermal effect is inevitable in upconversion particle assisted near-infrared polymerization (UCAP). Herein, the influence of thermal effects on the polymerization behavior are investigated. The effects of up-conversion particles content and NIR laser intensity on the polymerization rate and surface oxygen inhibition were systematically investigated, and the temperature evolution and complex viscosity changes in the polymerization system during the polymerization process were also monitored. In addition, polymerization experiments conducted on a controlled temperature platform were used to study the effect of NIR heating on the polymerization behavior. The results show that the near-infrared thermal effect promotes the polymerization reaction, but also causes severe oxygen inhibition which has an adverse effect on polymerization. Finally, NIR curing materials with enhanced mechanical properties than those of conventional UV curing materials were obtained.

12.
Front Immunol ; 12: 711980, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34594327

RESUMO

Regulatory B cells (Breg) are considered as immunosuppressive cells. Different subsets of Breg cells have been identified both in human beings and in mice. However, there is a lack of unique markers to identify Breg cells, and the heterogeneity of Breg cells in different organs needs to be further illuminated. In this study, we performed high-throughput single-cell RNA sequencing (scRNA-seq) and single-cell B-cell receptor sequencing (scBCR-seq) of B cells from the murine spleen, liver, mesenteric lymph nodes, bone marrow, and peritoneal cavity to better define the phenotype of these cells. Breg cells were identified based on the expression of immunosuppressive genes and IL-10-producing B (B10) cell-related genes, to define B10 and non-B10 subsets in Breg cells based on the score of the B10 gene signatures. Moreover, we characterized 19 common genes significantly expressed in Breg cells, including Fcrl5, Zbtb20, Ccdc28b, Cd9, and Ptpn22, and further analyzed the transcription factor activity in defined Breg cells. Last, a BCR analysis was used to determine the clonally expanded clusters and the relationship of Breg cells across different organs. We demonstrated that Atf3 may potentially modulate the function of Breg cells as a transcription factor and that seven organ-specific subsets of Breg cells are found. Depending on gene expression and functional modules, non-B10 Breg cells exhibited activated the TGF-ß pathway, thus suggesting that non-B10 Breg cells have specific immunosuppressive properties different from conventional B10 cells. In conclusion, our work provides new insights into Breg cells and illustrates their transcriptional profiles and BCR repertoire in different organs under physiological conditions.

13.
Virulence ; 12(1): 2764-2776, 2021 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-34699307

RESUMO

Dengue virus infection mainly causes dengue hemorrhagic fever (DHF) and/or dengue shock syndrome (DSS). However, ADE (antibody-dependent enhancement) is one of the main pathogenic factors, and its pathogenic mechanism has not been fully elucidated. Recently, with the development of high-throughput sequencing, an increased number of RNAs have been confirmed to play a vital regulatory role in the process of virus infection. However, there is a lack of research on dengue virus infection and ADE. In this study, we used RNA-Seq to detect differentially expressed RNAs (DE RNAs) profiles in mock-infected, DENV-3-infected, and ADE-infected THP-1 cells. Firstly, we found 69 circRNAs, 259 miRNAs, and 18 mRNAs were differentially expressed in THP-1 vs DENV-3. In THP-1 vs ADE, 94 circRNAs, 263 miRNAs, and 111 mRNAs were differentially expressed. In DENV-3 vs ADE, 68 circRNAs, 105 miRNAs, and 94 mRNAs were differentially expressed. Functional enrichment analysis of these DE RNAs mainly focused on immune system, viral infectious diseases, cytokine-cytokine receptor interactions, and NOD/RIG-I-like receptor signaling pathways. In DENV-3 vs ADE, notably, the expression of HBB was up-regulated, which was a Fcγ Receptor-mediated phagocytosis protein. Additionally, we predicted the encoding ability of DE circRNAs, and it was found that a small peptide was encoded by novel_circ_001562 and that its amino acid sequence was consistent with that of DDX60L, which is a class of interferon-stimulated genes. Finally, we constructed the ceRNA regulatory network pathway. Therefore, our study provides a new strategy for further investigation on DENV-host interactions.

14.
Knowl Based Syst ; 233: 107417, 2021 Dec 05.
Artigo em Inglês | MEDLINE | ID: mdl-34690447

RESUMO

In this study, a hybrid deep-learning model termed as ODANN, built upon neural networks (NN) coupled with data assimilation and natural language processing (NLP) features extraction methods, has been constructed to concurrently process daily COVID-19 time-series records and large volumes of COVID-19 related Twitter data, as representative of the global community's aggregated emotional responses towards the current pandemic, to model the growth rate in the number of confirmed COVID-19 cases globally via a proposed G parameter. Overall, there were 3 key components to ODANN's development phase, namely: (i) data hydration and pre-processing were performed on COVID-19 related Twitter data ranging between 23 January 2020 and 10 May 2020, which amounted to over 100 million Tweets written in English language; (ii) multiple NLP features extraction methods were subsequently leveraged to encode the hydrated Twitter data into useful semantic word vectors for training ODANN under an optimal set of hyperparameters; and (iii) historical time-series data of defined characteristics were also assimilated into ODANN's selected hidden layer(s) to model the G parameter daily with a lead-time of 1 day. By far, our experimental results demonstrated that by adopting a rolling time-window size of 5 days, with respect to the number of historical time-series records for assimilating different data features, enabled ODANN to outperform other traditional time-series models and recent studies, in terms of the computed RMSE and MAE scores attained from the model's testing step. Overall, the summarized results from ODANN demonstrated its competitive edge in modelling and forecasting the growth rate in the number of COVID-19 cases globally.

15.
Brain Lang ; 223: 105042, 2021 Oct 23.
Artigo em Inglês | MEDLINE | ID: mdl-34695614

RESUMO

Language recovery in aphasia is likely supported by a network of brain regions, but few studies have investigated treatment-related changes in functional connectivity while controlling for the absence of treatment. We examined functional connectivity in a 38-region picture-naming network in 30 patients with chronic aphasia who did or did not receive naming therapy. Compared to healthy controls, patients had abnormally low connectivity in a subset of connections from the naming network. Linear mixed models showed that the connectivity of abnormal connections increased significantly in patients who benefited from therapy, but not in those who did not benefit from or receive therapy. Changes in responders were specific to abnormal connections and did not extend to the larger network. Thus, successful naming therapy was associated with increased connectivity in connections that were abnormal prior to treatment. The potential to strengthen such connections may be a prerequisite for a successful treatment response.

16.
Proteomics ; 21(20): e2100007, 2021 10.
Artigo em Inglês | MEDLINE | ID: mdl-34482643

RESUMO

Methionine (Met) and cystine (CySS) are key sulfur donors in cell metabolism and are important nutrients for sustaining tumor growth; however, the molecular effects associated with their deprivation remain to be characterized. Here, we applied a xenograft mouse model to assess the impact of their deprivation on A549 xenografts and the xenograft-bearing animal. Results show that Met and CySS deprivation inhibits A549 growth in vitro, not in vivo. Deprivation was detrimental to the xenograft-bearing mouse, as demonstrated by weight loss and renal dysfunction. Differentially expressed proteins in A549 xenograft and mouse kidneys were characterized using quantitative proteomics. Functional annotation and protein-protein interaction network analysis revealed the enriched signaling pathways, including focal adhesion (Fn1) in the A549 xenograft, and xenobiotic metabolism (Cyp2e1) and glutathione metabolism (Ggt1) in the mouse kidney. Met and CySS deprivation inhibits the migratory and invasive properties of cancer cells, as evidenced by reduced expression of the epithelial to mesenchymal transition marker N-cadherin in A549 cells in vitro. Moreover, IGFBP1 protein expression was inhibited in both A549 xenograft and mouse kidneys. This study provides the first insights into changes within the proteome profile and biological processes upon Met and CySS deprivation in a A549 xenograft mouse model.


Assuntos
Cistina , Neoplasias Pulmonares , Animais , Transição Epitelial-Mesenquimal , Xenoenxertos , Metionina , Camundongos , Proteômica
17.
Bioorg Med Chem Lett ; 50: 128338, 2021 10 15.
Artigo em Inglês | MEDLINE | ID: mdl-34469710

RESUMO

7-Hydroxyneolamellarin A (7-OH-Neo A, 1), a natural marine product derived from sponge Dendrilla nigra, was first synthesized with 10% overall yield under the instruction of convergent synthetic strategy. We found that 7-OH-Neo A could attenuate the accumulation of hypoxia-inducible factor-1α (HIF-1α) protein and inhibit vascular epidermal growth factor (VEGF) transcriptional activity, showing well inhibitory effect on HIF-1 signaling pathway. Meantime, 7-OH-Neo A had the well anti-tumor activities, such as inhibiting tumor angiogenesis, proliferation, migration and invasion. More importantly, 7-OH-Neo A exhibited profound anti-tumor effect in mice breast cancer model by suppressing the accumulation of HIF-1α in tumor tissue. Mechanism study demonstrated that 7-OH-Neo A might target the protein with the ability of stabilizing HIF-1α in hypoxia. Due to the excellent water solubility, superior anti-tumor activity and good biocompatibility, 7-OH-Neo A shows the promising potential for being exploited as an anti-tumor agent in near future.

18.
Sustain Cities Soc ; 75: 103254, 2021 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-34414067

RESUMO

To inform data-driven decisions in fighting the global pandemic caused by COVID-19, this research develops a spatiotemporal analysis framework under the combination of an ensemble model (random forest regression) and a multi-objective optimization algorithm (NSGA-II). It has been verified for four Asian countries, including Japan, South Korea, Pakistan, and Nepal. Accordingly, we can gain some valuable experience to better understand the disease evolution, forecast the prevalence of the disease, which can provide sustainable evidence to guide further intervention and management. Random forest with a proper rolling time-window can learn the combined effects of environmental and social factors to accurately predict the daily growth of confirmed cases and daily death rate on a national scale, which is followed by NSGA-II to find a range of Pareto optimal solutions for ensuring the minimization of the infection rate and mortality at the same time. Experimental results demonstrate that the predictive model can alert the local government in advance, allowing the accused time to put forward relevant measures. The temperature in the category of environment and the stringency index belonging to the social factor are identified as the top 2 important features to exert a greater impact on the virus transmission. Moreover, optimal solutions provide references to design the best control strategies towards pandemic containment and prevention that can accommodate the country-specific circumstance, which are possible to decrease the two objectives by more than 95%. In particular, appropriate adjustment of social-related features needs to take priority over others, since it can bring about at least 1.47% average improvement of two objectives compared to environmental factors.

19.
J Agric Food Chem ; 69(35): 10104-10113, 2021 Sep 08.
Artigo em Inglês | MEDLINE | ID: mdl-34449210

RESUMO

Lipid digestion characteristics in human, bovine, and caprine milk were investigated using an infant in vitro digestion model. Our results suggested that particle size in bovine and caprine milk increased initially and then decreased over time, whereas the particle size in human milk continuously decreased. The lipolysis degree of human milk (86.8%) was higher than that in bovine (80.2%) and caprine (82.7%) milk (P < 0.05). Compared to human milk, bovine and caprine milk released higher unsaturated fatty acids and lower SFAs. In addition, 12 and 84 glyceride species were significantly different between bovine and human milk, during gastrointestinal digestion (P < 0.05). Another 13 and 92 glyceride species were found to be significantly different between caprine and human milk. A total of 30 and 31 lipids were screened as biomarkers to further clarify the differences related to lipid digestion properties of human, bovine, and caprine milk.


Assuntos
Cabras , Leite , Animais , Bovinos , Digestão , Humanos , Lactente , Fórmulas Infantis , Lipídeos , Leite Humano
20.
Autophagy ; : 1-2, 2021 Aug 30.
Artigo em Inglês | MEDLINE | ID: mdl-34455934

RESUMO

For network rewiring and information storage in the brain, late phase long-term synaptic depression (L-LTD) requires the long-lasting reorganization of cellular resources. We found that activation of GRIN/NMDAR recruits transcription-dependent autophagy for synaptic turnover to support L-LTD. Activity-dependent CRTC1 synapto-nuclear translocation increases nuclear CRTC1 that competes with FXR for binding to CREB; this in turn enhances the direct binding between CRTC1-CREB and macroautophagy/autophagy gene promoters. Synergistic actions of CRTC1-CREB are preferentially turned on by LTD-inducing stimuli and switched off by genetic knockdown of CREB or CRTC1, or acutely activating FXR. Disrupted CRTC1-CREB signaling impairs activity-driven loss of surface GRIA/AMPARs and DLG4/PSD-95, and selectively prevents GRIN/NMDAR-dependent L-LTD, which are rescued by enhancing MTOR-regulated autophagy. These findings suggest a novel mechanism in L-LTD, in which brief synaptic activities recruit long-lasting autophagy through excitation-transcription coupling for ensuing synaptic remodeling.

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