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1.
J Vet Med Sci ; 82(9): 1379-1386, 2020 Oct 07.
Artigo em Inglês | MEDLINE | ID: mdl-32713876

RESUMO

This study aimed to investigate the distribution and epidemiological relatedness of methicillin-resistant Staphylococcus aureus (MRSA) isolates from companion dogs, owners, and residential environments of 72 households. Sampling was performed twice from January to June 2018 and a total of 2,592 specimens were collected. The specimens collected from each household were streaked on CHROM agar S. aureus and the colonies grown on the medium were further identified using a mass spectrometry microbial identification system. Antimicrobial susceptibility testing, Panton-Valentine-Leukocidin (PVL) gene PCR, staphylococcal cassette chromosome mec (SCCmec) typing, Staphylococcus aureus Protein A (spa) typing, pulsed-field gel electrophoresis (PFGE), and multi-locus sequence typing (MLST) were conducted to evaluate the phenotypic and genotypic characteristics of the MRSA isolates. A total of 65 S. aureus strains (2.5%) were isolated and 49 (1.9%) of 65 strains were MRSA displaying cefoxitin-resistance with mecA carriage. MRSA strains were isolated from dogs (n=6, 9.2%), owners (n=27, 41.5%), and residential environments (n=16, 24.6%), respectively. Overall prevalence of non-duplicated MRSA was 16.7% (12/72 households) at household level. ST72-SCCmec IVc MRSA clones predominantly appeared in MRSA-positive families. Furthermore, PFGE analyses showed that ST72-SCCmec IVc-t324 is shared between dog owners and dogs. To our knowledge, this is the first study to report the sharing of ST72 MRSA between dogs and their owners.

2.
J Vet Sci ; 21(3): e40, 2020 May.
Artigo em Inglês | MEDLINE | ID: mdl-32476314

RESUMO

The purpose of this study was to investigate the high-level mupirocin resistance (HLMR) in Gram-positive bacteria isolated from companion animals. A total of 931 clinical specimens were collected from diseased pets. The detection of mupirocin-resistant bacteria and plasmid-mediated mupirocin resistance genes were evaluated by antimicrobial susceptibility tests, polymerase chain reactions, and sequencing analysis. Four-hundred and six (43.6%) bacteria were isolated and 17 (4.2%), including 14 staphylococci and 3 Corynebacterium were high-level mupirocin-resistant (MICs, ≥ 1,024 ug/mL) harboring mupA. Six staphylococci of HLMR strains had plasmid-mediated mupA-IS257 flanking regions. The results show that HLMR bacteria could spread in veterinary medicine in the near future.


Assuntos
Antibacterianos/farmacologia , Corynebacterium/efeitos dos fármacos , Farmacorresistência Bacteriana , Mupirocina/farmacologia , Staphylococcus/efeitos dos fármacos , Animais , Doenças do Gato/tratamento farmacológico , Gatos , Infecções por Corynebacterium/tratamento farmacológico , Doenças do Cão/tratamento farmacológico , Cães , Testes de Sensibilidade Microbiana/veterinária , Reação em Cadeia da Polimerase/veterinária , Análise de Sequência de DNA/veterinária , Infecções Estafilocócicas/tratamento farmacológico
3.
J Vet Sci ; 21(3): e42, 2020 May.
Artigo em Inglês | MEDLINE | ID: mdl-32476316

RESUMO

Regenerative medicine using stem cells from various sources are emerging treatment modality in several refractory diseases in veterinary medicine. It is well-known that stem cells can differentiate into specific cell types, self-renew, and regenerate. In addition, the unique immunomodulatory effects of stem cells have made stem cell transplantation a promising option for treating a wide range of disease and injuries. Recently, the medical demands for companion animals have been rapidly increasing, and certain disease conditions require alternative treatment options. In this review, we focused on stem cell application research in companion animals including experimental models, case reports and clinical trials in dogs and cats. The clinical studies and therapeutic protocols were categorized, evaluated and summarized according to the organ systems involved. The results indicate that evidence for the effectiveness of cell-based treatment in specific diseases or organ systems is not yet conclusive. Nonetheless, stem cell therapy may be a realistic treatment option in the near future, therefore, considerable efforts are needed to find optimized cell sources, cell numbers and delivery methods in order to standardize treatment methods and evaluation processes.


Assuntos
Doenças do Gato/terapia , Doenças do Cão/terapia , Transplante de Células-Tronco/veterinária , Animais , Doenças do Gato/cirurgia , Gatos , Doenças do Cão/cirurgia , Cães , Transplante de Células-Tronco/estatística & dados numéricos , Células-Tronco/fisiologia
4.
Front Microbiol ; 11: 674, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32351490

RESUMO

This study aimed to describe the distribution and characterization of fecal extended-spectrum ß-lactamase (ESBL)- and AmpC-producing Escherichia coli isolates from healthy companion animals and cohabiting humans. A total of 968 rectal swab samples from 340 participants, including healthy companion animals and cohabiting humans, were collected from 130 households in South Korea from 2018 to 2019. To determine the bacterial profiles of the participants, several experiments were performed as follows: antimicrobial susceptibility testing, PCR and direct sequencing for ESBL/AmpC production, PFGE, MLST, whole genome sequencing and qRT-PCR. A total of 24.9 and 21.5% of the E. coli isolates from healthy companion animals and cohabiting humans were ESBL/AmpC producers, respectively. The bla CTX-M- 14 gene was the most prevalent ESC resistance gene in both pets (n = 25/95, 26.3%) and humans (n = 44/126, 34.9%). The bla CMY- 2 gene was also largely detected in pets (n = 19, 20.0%). Overall, intrahousehold pet-human sharing of ESBL/AmpC E. coli isolates occurred in 4.8% of households, and the isolates were all CTX-M-14 producers. In particular, ten CMY-2-producing E. coli isolates from seven dogs and three humans in the different households belonged to the same pulsotype. The MIC values of cefoxitin and the transcription level in CMY-2-producing E. coli isolates were proportional to the bla CMY- 2 copy number on the chromosome. Our results showed that the clonal spread of fecal ESBL/AmpC-producing E. coli households' isolates between healthy companion animals and cohabiting humans was rare, but it could happen. In particular, E. coli ST405 isolates carrying multiple bla CMY- 2 genes on the chromosome was sporadically spread between companion animals and humans in South Korea.

6.
BMC Vet Res ; 16(1): 3, 2020 Jan 03.
Artigo em Inglês | MEDLINE | ID: mdl-31900158

RESUMO

BACKGROUND: Pheochromocytoma (PCC) has poor prognosis and adrenalectomy is hard to be performed, in case of caudal vena cava invasion. The long-term administration of phenoxybenzamine in PCC has not been reported in dogs. CASE PRESENTATION: A 14-year-old castrated male Poodle dog presented with an abdominal mass. On physical examination, hypertension, increased lens opacity, calcinosis cutis, generalized alopecia, and systolic murmur were observed. Serum chemistry and urinalysis profiles revealed hyperglycemia, hypercholesterolemia, elevated liver enzymes, and glucosuria. Abdominal ultrasonography showed a right adrenal mass with invasion of the caudal vena cava, which was cytologically diagnosed as suspected PCC. An adrenal mass (width × height × length, 28 × 26 × 48 mm3) was found on computed tomography and diagnosed as PCC with increased plasma metanephrines and normetanephrines. An adrenocorticotropin hormone stimulation test showed elevated adrenal hormones (androstenedione, estradiol, progesterone, and 17-OH progesterone) with normal cortisol, compatible with atypical Cushing's syndrome. The dog was managed with trilostane, phenoxybenzamine, and insulin therapy. Glycosylated hemoglobin and fructosamine levels gradually decreased, and hypertension resolved. In the 10-month follow-up period, the liver enzymes levels gradually decreased, and the clinical signs of the dog were well-controlled without deterioration. CONCLUSIONS: This case report describes long-term medical management without adrenalectomy of PCC complicated with atypical Cushing's syndrome and DM.


Assuntos
Neoplasias das Glândulas Suprarrenais/veterinária , Doenças do Cão/diagnóstico , Feocromocitoma/veterinária , Neoplasias das Glândulas Suprarrenais/diagnóstico , Antagonistas Adrenérgicos alfa/uso terapêutico , Animais , Antineoplásicos/uso terapêutico , Síndrome de Cushing , Diabetes Mellitus/tratamento farmacológico , Di-Hidrotestosterona/análogos & derivados , Di-Hidrotestosterona/uso terapêutico , Doenças do Cão/tratamento farmacológico , Cães , Hipoglicemiantes/uso terapêutico , Insulina/uso terapêutico , Masculino , Fenoxibenzamina/uso terapêutico , Feocromocitoma/diagnóstico , Feocromocitoma/tratamento farmacológico , Resultado do Tratamento
7.
Sci Data ; 6(1): 147, 2019 08 14.
Artigo em Inglês | MEDLINE | ID: mdl-31413331

RESUMO

Studies of naturally occurring cancers in dogs, which share many genetic and environmental factors with humans, provide valuable information as a comparative model for studying the mechanisms of human cancer pathogenesis. While individual and small-scale studies of canine cancers are underway, more generalized multi-omics studies have not been attempted due to the lack of large-scale and well-controlled genomic data. Here, we produced reliable whole-exome and whole-transcriptome sequencing data of 197 canine mammary cancers and their matched controls, annotated with rich clinical and biological features. Our dataset provides useful reference points for comparative analysis with human cancers and for developing novel diagnostic and therapeutic technologies for cancers in pet dogs.


Assuntos
Cães/genética , Exoma , Neoplasias Mamárias Animais/genética , Transcriptoma , Animais , Feminino , Sequenciamento Completo do Exoma
8.
Front Microbiol ; 10: 1371, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31275286

RESUMO

Extended-spectrum cephalosporin (ESC)-resistant Enterobacteriaceae is an increasingly important problem in both human and veterinary medicine. The aims of this study were to describe a comparative molecular characterization of Enterobacteriaceae carrying ESC resistance genes, encoding extended-spectrum ß-lactamase (ESBL) and AmpC, isolated from human stool samples, rectal swabs from companion animals, and swabs from the environment of veterinarian hospitals in South Korea, and to examine their possible dissemination and transmission. The ESC resistance genes were identified by PCR and sequencing. Isolates with the predominant ESC resistance genes were assessed for their genetic relatedness by pulsed-field gel electrophoresis (PFGE) and multi-locus sequence typing. A total of 195 Escherichia coli and 41 Klebsiella pneumoniae isolates that exhibited ESC resistance were recovered on CHROMagar ESBL from human, companion animal, and the veterinary hospital environmental samples. In companion animals, most of the ESC resistance genes were bla CMY-2-like (26.4%), followed by bla CTX -M-55 (17.2%) and bla CTX-M-14 (16.1%), whereas bla CTX-M-15 (28.6%) was predominant in human samples. The epidemiological relatedness of isolates carrying ESC resistance genes, including 124 E. coli and 23 K. pneumoniae isolates carrying CMY-2-like, DHA-1-like, or/and CTX-M-type, were analyzed by PFGE. The pulsotypes of five E. coli isolates (three from dogs and two from humans) carrying bla CMY-2-like, which were attributed to sequence type 405, from different veterinary clinics showed >85% similarity. Our results indicate direct transmission and dissemination of ESC-resistant Enterobacteriaceae between humans and companion animals.

9.
Acta Vet Scand ; 61(1): 22, 2019 May 06.
Artigo em Inglês | MEDLINE | ID: mdl-31060620

RESUMO

BACKGROUND: Bite wounds are one of the most common traumatic injuries in dogs and depending on their severity, location, etc., urgent care including antibiotic therapy may be necessary. Serious complications can result from these injuries, such as multiple organ dysfunction syndrome (MODS), as well as a generalized reduction in cerebral perfusion, e.g. during cardiac arrest, shock, or severe hypotension that may cause global brain ischemia (GBI). CASE PRESENTATION: A 5-year-old spayed female Maltese dog was presented with generalized seizures, ataxia, and obtunded mentation. The dog was injured by severe bite wounds that penetrated its abdomen and had received blood transfusions, antibiotic therapy (including metronidazole and cefazoline) and underwent emergency surgery 4 days before its visit. Based on a clinical examination, intracranial hypoxic damage with elevated intra-cranial pressure and MODS were highly suspected, and GBI was confirmed following magnetic resonance imaging. Increased signal intensity diffusely distributed in the olfactory bulb and frontal, temporal, and parietal grey matter was evident on the T2-weighted and fluid attenuated inversion recovery transverse images, along with corresponding high signal intensity observed on diffusion weighted imaging. During the 10-month follow-up period, the clinical signs gradually improved, but intermittent circling and cognitive dysfunction deficits remained. CONCLUSIONS: GBI should be included among the differential diagnoses in case of any peracute non-progressive neurological dysfunction that occurs with episodes of hypotension or hypoxia. The abnormal signal intensity observed on diffusion weighted imaging was a useful indicator for diagnosing this condition. Long-term medical management with antibiotics and anti-convulsant and anti-oxidant therapies were considered to be helpful in managing the GBI concurrent with MODS in this dog.


Assuntos
Mordeduras e Picadas/veterinária , Isquemia Encefálica/veterinária , Doenças do Cão/etiologia , Insuficiência de Múltiplos Órgãos/veterinária , Animais , Mordeduras e Picadas/complicações , Mordeduras e Picadas/terapia , Isquemia Encefálica/tratamento farmacológico , Isquemia Encefálica/etiologia , Doenças do Cão/patologia , Doenças do Cão/terapia , Cães , Feminino , Hidrocefalia/tratamento farmacológico , Hidrocefalia/veterinária , Insuficiência de Múltiplos Órgãos/etiologia , Insuficiência de Múltiplos Órgãos/patologia , Insuficiência de Múltiplos Órgãos/terapia
10.
J Vet Sci ; 20(1): 63-71, 2019 Jan 31.
Artigo em Inglês | MEDLINE | ID: mdl-30541184

RESUMO

Genome-wide association study (GWAS) is a powerful tool for identifying the genetic causes of various diseases. This study was conducted to identify genomic variation in Maltese dog genomes associated with degenerative mitral valve disease (DMVD) development and to evaluate the association of each biological condition with DMVD in Maltese dogs. DNA was extracted from blood samples obtained from 48 Maltese dogs (32 with DMVD and 16 controls). Genome-wide single nucleotide polymorphism (SNP) genotyping was performed. The top 30 SNPs from each association of various conditions and genetic variations were mapped to their gene locations. A total of 173,662 loci were successfully genotyped, with an overall genotype completion rate of 99.41%. Quality control analysis excluded 46,610 of these SNPs. Manhattan plots were produced using allelic tests with various candidate clinical conditions. A significant peak of association was observed between mitral valve prolapse (MVP) and SNPs on chromosome 17. The present study revealed significant SNPs in several genes associated with cardiac function, including PDZ2, Armadillo repeat protein detected in velo-cardio-facial syndrome, catenin (cadherin-associated protein) alpha 3, low-density lipoprotein receptor class A domain containing protein 4, and sterile alpha motif domain containing protein 3. To our knowledge, this is the first study of a genetic predisposition to DMVD in Maltese dogs. Although only a limited number of cases were analyzed, these data could be the basis for further research on the genetic predisposition to MVP and DMVD in Maltese dogs.


Assuntos
Doenças do Cão/genética , Predisposição Genética para Doença , Estudo de Associação Genômica Ampla/veterinária , Doenças das Valvas Cardíacas/veterinária , Valva Mitral/patologia , Polimorfismo de Nucleotídeo Único , Animais , Cães , Feminino , Genótipo , Doenças das Valvas Cardíacas/genética , Masculino
11.
Microb Drug Resist ; 25(3): 344-349, 2019 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-30379599

RESUMO

The gene for New Delhi metallo-ß-lactamase-5 (NDM-5) in Escherichia coli has been identified in many countries mainly from human clinical specimens. The isolates carrying this gene are even more rarely isolated from companion animals. In this study, four carbapenem-resistant isolates were recovered from four dogs in Korea. All isolates carried blaNDM-5 and exhibited resistance to meropenem and imipenem, and were susceptible to colistin. Epidemiological analysis showed that all four isolates were sequence type 410 (ST410) and shared 99% similarity as determined by pulsed-field gel electrophoresis analysis. Among the four isolates, the Z0117EC0033 strain was randomly selected for whole-genome sequencing, composed of a 4.7Mb circularized chromosome carrying the CMY-2 gene and two plasmids. The first plasmid of the IncFIB type had 83 coding sequences (CDS) in ca. 74 kb. The second smaller plasmid of the IncX3 type had 57 CDS and carried only the blaNDM-5 gene in ca. 46 kb. The plasmid structures were highly similar (> 99%) to those of the NDM-5 human-like IncX3 plasmid. This is the first report of carbapenemase-producing Enterobacteriaceae from companion animals in Korea. The human-like blaNDM-5 IncX3 plasmid identified in this study suggests a potential transmission route of the NDM-5 plasmid between humans and companion animals.


Assuntos
Escherichia coli/genética , Escherichia coli/isolamento & purificação , Animais de Estimação/microbiologia , beta-Lactamases/genética , Animais , Antibacterianos/farmacologia , Farmacorresistência Bacteriana Múltipla/efeitos dos fármacos , Farmacorresistência Bacteriana Múltipla/genética , Escherichia coli/efeitos dos fármacos , Infecções por Escherichia coli/tratamento farmacológico , Infecções por Escherichia coli/microbiologia , Humanos , Testes de Sensibilidade Microbiana/métodos , Plasmídeos/genética , República da Coreia
12.
BMC Vet Res ; 14(1): 382, 2018 Dec 04.
Artigo em Inglês | MEDLINE | ID: mdl-30514314

RESUMO

BACKGROUND: There is no specific therapy for cutaneous epitheliotropic T-cell lymphoma (CETL). The administration of retinoids in conjunction with interferon-α (IFN-α) in CETL has not been reported in dogs. CASE PRESENTATION: Two dogs (Shih tzu and Miniature pinscher) presented with multiple nodular skin lesions. Histopathological examination revealed diffuse infiltrations of lymphocytes in the epidermis and dermis, with a CD3-positive immunophenotypic profile. Based on the clinical and histopathological examination, CETL was diagnosed. Both dogs were treated with isotretinoin in combination with IFN-α and showed clinical improvement with complete or partial remission. The disease in these dogs was well-controlled for more than 264 days of overall median survival time without any additional clinical signs after initiation of the treatment. In both the cases, the dogs were followed up for 27 months, and 10 months without any evidence of recurrence or metastasis, respectively. CONCLUSIONS: We describe the clinical efficacy of isotretinoin combined with IFN-α in 2 dogs with CETL. Long-term management with isotretinoin combined with IFN-α was effective in treating CETL in these cases.


Assuntos
Doenças do Cão/tratamento farmacológico , Interferon-alfa/uso terapêutico , Isotretinoína/uso terapêutico , Linfoma Cutâneo de Células T/veterinária , Animais , Antineoplásicos/uso terapêutico , Doenças do Cão/patologia , Cães , Feminino , Linfoma Cutâneo de Células T/tratamento farmacológico , Linfoma Cutâneo de Células T/patologia , Masculino , Resultado do Tratamento
13.
J Vet Res ; 62(2): 229-235, 2018 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-30364817

RESUMO

Introduction: p63 is a homologous molecule of p53 and was recently identified as playing important roles in several key cellular processes, including epithelial development and proliferation. Since then, several studies have demonstrated altered p63 expression in various cancers of epithelial origin. Canine perianal gland tumour is one of the most common skin neoplasms in dogs; however, the molecular characteristics of this tumour remain poorly understood. The objective of the present study was to analyse and compare the expression of p63 in canine perianal gland adenomas and carcinomas. Material and Methods: Haematoxylin and eosin-stained slides were examined and immunohistochemistry was conducted for a total of 65 samples. Immunohistochemical data for p63 expressions were compared between groups using the Mann-Whitney U test. Results: The p63 expression level was increased in perianal gland carcinomas compared to that in the adenoma samples (P < 0.0001). The percentage of cells expressing p63 was higher in perianal gland carcinomas than in adenomas, although the intensity of immunostaining did not differ significantly between the two groups. Conclusion: p63 is a candidate factor contributing to the malignant transformation and progression of canine perianal gland tumours.

14.
J Vet Diagn Invest ; 30(3): 386-391, 2018 May.
Artigo em Inglês | MEDLINE | ID: mdl-29429401

RESUMO

Worsening renal function and azotemia in patients with heart failure (HF) are strongly associated with disease severity and poor prognosis. Increasing interest in this correlation led to the description and classification of cardiorenal syndrome (CRS). We evaluated the role of neutrophil gelatinase-associated lipocalin (NGAL) in the early detection of CRS in dogs with HF. Ten healthy dogs and 31 dogs admitted with HF were included in our study. NGAL and troponin-I were measured on samples collected on the day of admission; creatinine was measured on admission and again on day 7. The CRS group was defined as subsequently developing renal azotemia. Of 31 dogs with HF, 20 were included in the HF group, and 11 were included in the CRS group. The admission NGAL concentrations of the CRS group were significantly higher than those of other groups ( p < 0.001). The severity of HF evaluation based on the modified New York Heart Association classification showed significant correlation with NGAL ( p < 0.001) and troponin-I ( p = 0.009) concentration. However, only serum NGAL concentration at admission was significantly associated with the development of CRS in dogs with HF ( p = 0.021). The admission serum NGAL ≥ 16.0 ng/mL (optimal cutoff value) had a sensitivity of 90.9% and specificity of 90.0% in predicting the development of CRS.


Assuntos
Biomarcadores/sangue , Síndrome Cardiorrenal/veterinária , Doenças do Cão/diagnóstico , Lipocalina-2/sangue , Animais , Síndrome Cardiorrenal/diagnóstico , Estudos de Casos e Controles , Doenças do Cão/sangue , Cães , Ensaio de Imunoadsorção Enzimática/veterinária , Feminino , Masculino , Sensibilidade e Especificidade
15.
J Vet Sci ; 19(1): 129-135, 2018 Jan 31.
Artigo em Inglês | MEDLINE | ID: mdl-28693307

RESUMO

Degenerative mitral valve disease (DMVD) is the most commonly acquired cardiac disease in dogs. This study evaluated the relationship between genetic variations in the serotonin transporter (SERT) gene of Maltese dogs and DMVD. Genomic DNA was extracted from blood samples collected from 20 client-owned DMVD Maltese dogs and 10 healthy control dogs, and each exon of the SERT gene was amplified via polymerase chain reaction. The resulting genetic sequences were aligned and analyzed for variations by comparing with reference sequences; the predicted secondary structures of these variations were modeled and cross-verified by applying computational methods. Genetic variations, including five nonsynonymous genetic variations, were detected in five exons. Protein structure and function of the five nonsynonymous genetic variations were predicted. Three of the five polymorphisms were predicted to be probable causes of damage to protein function and confirmed by protein structure model verification. This study identified six polymorphisms of the SERT gene in Maltese dogs with DMVD, suggesting an association between the SERT gene and canine DMVD. This is the first study of SERT mutation in Maltese dogs with DMVD and is considered a pilot study into clinical genetic examination for early DMVD diagnosis.


Assuntos
Doenças do Cão/genética , Doenças das Valvas Cardíacas/veterinária , Polimorfismo Genético , Proteínas da Membrana Plasmática de Transporte de Serotonina/genética , Animais , Doenças do Cão/patologia , Cães , Feminino , Doenças das Valvas Cardíacas/genética , Doenças das Valvas Cardíacas/patologia , Masculino , Valva Mitral/patologia , Projetos Piloto , Proteínas da Membrana Plasmática de Transporte de Serotonina/metabolismo
16.
Vet Q ; 38(1): 14-20, 2018 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-29135361

RESUMO

BACKGROUND: High prevalence of methicillin resistance among clinical isolates of Staphylococcus pseudintermedius obtained from dogs was reported in Seoul metropolitan area, South Korea. However, no information on genetic lineage and clonal spread is currently available. OBJECTIVE: The aim is to identify the genetic diversity of methicillin-resistant or -susceptible S. pseudintermedius (MRSP and MSSP, respectively) from healthy dogs. ANIMALS AND METHODS: From 119 healthy dogs, 29 isolates consisting of 20 MRSP and 9 MSSP were collected from June 2013 to February 2014. Phenotypic features, antibiogram, multilocus sequence type (MLST), Staphylococcal cassette chromosome mec (SCCmec) type and spa gene type were analyzed. RESULTS: MLST showed 24 sequence types (STs), including 20 new STs that were genetically distinct from the previous STs in other geographic areas. SCCmec typing revealed that all isolates had SCCmec type V, a predominant type in North America. spa gene typing was successful in only 13 isolates (10 MRSP and 3 MSSP) and revealed two known types (t02 and t06), as well as one novel type (t73). CONCLUSION: Our cumulative data indicate the presence of various populations of S. pseudintermedius in clinically normal dogs in Seoul metropolitan area.


Assuntos
Doenças do Cão/microbiologia , Resistência a Meticilina/genética , Infecções Estafilocócicas/veterinária , Staphylococcus/genética , Animais , Antibacterianos/farmacologia , Doenças do Cão/tratamento farmacológico , Doenças do Cão/genética , Cães , Farmacorresistência Bacteriana Múltipla/genética , Modelos Logísticos , Tipagem de Sequências Multilocus/veterinária , Mucosa Nasal/microbiologia , República da Coreia , Infecções Estafilocócicas/tratamento farmacológico , Infecções Estafilocócicas/genética , Infecções Estafilocócicas/microbiologia , Staphylococcus/efeitos dos fármacos , Staphylococcus/isolamento & purificação
17.
Am J Vet Res ; 78(4): 440-446, 2017 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-28345991

RESUMO

OBJECTIVE To measure serum homocysteine concentrations in dogs with myxomatous mitral valve disease (MMVD) and identify any association between this variable and stage of MMVD. ANIMALS 53 client-owned dogs with MMVD and 10 healthy control Beagles. PROCEDURES Dogs with MMVD were allocated to 3 groups in accordance with the staging system for chronic valvular heart disease in dogs and cats of the American College of Veterinary Internal Medicine. Blood samples were collected from all dogs, and serum homocysteine and cardiac troponin 1 concentrations were measured by enzyme immunoassay and chemiluminescence immunoassay, respectively. Analyte values were tested for associations with each other and with stage of MMVD. RESULTS A significant correlation was identified between serum homocysteine concentration and stage of MMVD. Mean ± SD concentrations were 6.72 ± 1.65 µmol/L for control dogs, 13.37 ± 4.16 µmol/L for dogs with stage B MMVD, 18.86 ± 6.73 µmol/L for dogs with stage C disease, and 28.26 ± 4.48 µmol/L for dogs with stage D disease. In addition, serum homocysteine concentration was correlated with serum cardiac troponin 1 (r = 0.34) and creatinine (r = 0.46) concentrations, systolic blood pressure (r = 0.57), and left atrium-to-aortic root ratio (r = 0.28), all of which were positively correlated with stage of MMVD. CONCLUSIONS AND CLINICAL RELEVANCE Serum homocysteine concentrations of dogs with MMVD were significantly higher than those of control dogs, and significant correlations were identified between these values and several risk factors for heart failure. Measurement of serum homocysteine concentration may be useful in the prediction of severity of disease in dogs with MMVD.


Assuntos
Doenças do Cão/sangue , Doenças das Valvas Cardíacas/veterinária , Homocisteína/sangue , Valva Mitral , Animais , Pressão Sanguínea , Creatinina/sangue , Cães , Feminino , Insuficiência Cardíaca/sangue , Doenças das Valvas Cardíacas/sangue , Masculino , Troponina I/sangue
18.
Vet J ; 212: 15-21, 2016 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-27256020

RESUMO

This study investigated colonization and association of staphylococci between healthy dogs and their owners. In a cross-sectional study, nasal carriage and antibiotic susceptibility of isolated staphylococci were determined for 119 dogs and 107 owners. Relatedness of the Staphylococcus isolates in dogs and their owners was investigated using antibiograms, toxin profiles, and genotyping by pulsed-field gel electrophoresis (PFGE), multilocus sequence type, and spa typing. Risk factors for carriage of methicillin-resistant staphylococci in dogs were also evaluated. Staphylococcus spp. were isolated from 65 (60.7%) owners and 44 (37.0%) dogs. The following species were isolated, listed in order of decreasing frequency: S. epidermidis, S. pseudintermedius, S. aureus, S. scheiferi subsp. coagulans, S. haemolyticus, S. sciuri, S. saprophyticus and S. warneri. S. pseudintermedius (65.9%) was the major isolate in dogs while S. epidermidis (81.5%) was the major type in owners. Among the isolates, 71.6% were methicillin resistant (MR) and 95.4% of the isolates demonstrated multi-drug resistance regardless of the origin. Only one dog-owner pair shared the same Staphylococcus spp. (S. pseudintermedius); however, the organisms were of different PFGE subtypes and exhibited different antibiotic resistance and toxin profiles while both isolates displayed same sequence type (ST365). While the dog-origin isolate showed spa type t02, the owner-origin isolate was negative to PCRs targeting spa gene sequence. Risk factor analysis showed that the presence of cohabitant animals was correlated with the nasal carriage of MR staphylococci in dogs. The cumulative data indicated that animal- and owner-origin staphylococci have various subtypes with high prevalence of MR; however, the bacteria are not shared between healthy dogs and their owners.


Assuntos
Antibacterianos/farmacologia , Doenças do Cão/epidemiologia , Farmacorresistência Bacteriana Múltipla , Infecções Estafilocócicas/epidemiologia , Infecções Estafilocócicas/veterinária , Staphylococcus/isolamento & purificação , Adolescente , Adulto , Idoso , Animais , Portador Sadio , Estudos Transversais , Doenças do Cão/microbiologia , Cães , Feminino , Genótipo , Humanos , Masculino , Testes de Sensibilidade Microbiana/veterinária , Pessoa de Meia-Idade , Nariz/microbiologia , Prevalência , República da Coreia/epidemiologia , Fatores de Risco , Infecções Estafilocócicas/microbiologia , Adulto Jovem
19.
Am J Vet Res ; 77(2): 199-207, 2016 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-27027715

RESUMO

OBJECTIVE: To evaluate the effects of granulocyte colony-stimulating factor (GCSF) administration in dogs with experimentally induced acute kidney injury. ANIMALS: 6 healthy dogs. PROCEDURES: After induction of kidney injury (day 0) with cisplatin (5 mg/kg, IV), the dogs were randomly assigned into 2 groups (n = 3 dogs/group). Then dogs immediately received GCSF (10 µg/kg) or 1 mL of saline (0.9% NaCl) solution (control group) SC; this treatment was repeated once daily for 4 additional days (days 1 through 4). A once-daily CBC (day 0 to 4), serum biochemical analysis (day 0 to 3), and urinalysis (day 0 to 3) were performed for each dog; samples were collected before administration of cisplatin (day 0) and before treatment with GCSF or saline solution (days 1 through 4). After sample collection and treatment on day 4, all dogs were euthanized; kidney tissue samples underwent histologic evaluation, immunohistochemical analyses, and cytokine profiling via reverse transcriptase PCR assay. RESULTS: In the GCSF-treated group, the histologic evaluation and immunohistochemical analyses of kidney tissue revealed less fibrotic change and greater proliferation of renal tubular epithelial cells, compared with findings in the control group. The mRNA profiles of kidney tissue from the GCSF-treated group indicated lower expression of tumor necrosis factor-α and tumor growth factor-ß, compared with findings in the control group; however, concentrations of factors related to renal regeneration were not greater in the GCSF-treated group. CONCLUSIONS AND CLINICAL RELEVANCE: Results indicated that GCSF treatment can impede renal fibrosis and increase proliferation of renal tubules after experimentally induced acute kidney injury in dogs.


Assuntos
Lesão Renal Aguda/veterinária , Doenças do Cão/induzido quimicamente , Fator Estimulador de Colônias de Granulócitos/farmacologia , Regeneração/efeitos dos fármacos , Lesão Renal Aguda/induzido quimicamente , Lesão Renal Aguda/tratamento farmacológico , Animais , Cisplatino/toxicidade , Doenças do Cão/tratamento farmacológico , Cães , Fator Estimulador de Colônias de Granulócitos/administração & dosagem , Rim/efeitos dos fármacos , Fator de Necrose Tumoral alfa
20.
Am J Vet Res ; 77(2): 208-17, 2016 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-27027716

RESUMO

OBJECTIVE: To evaluate the usefulness of autologous bone marrow-derived mesenchymal stem cell (BM-MSC) therapy for the treatment of dogs with experimentally induced acute kidney injury. ANIMALS: 6 healthy dogs. PROCEDURES: After induction of kidney injury (day 0) with cisplatin (5 mg/kg, IV), dogs immediately received saline (0.9% NaCl) solution (10 mL; n = 3) or BM-MSCs (1 × 10(6) cells/kg in 10 mL of saline solution; 3) IV. A CBC, serum biochemical analysis, and urinalysis were performed for each dog before administration of cisplatin and on days 1 through 4. Glomerular filtration rate was determined for all dogs on days -7 and 2; BM-MSC tracking by MRI was performed on BM-MSC-treated dogs on days -14 and 4. After sample collection and BM-MSC tracking on day 4, all dogs were euthanized; kidney tissue samples underwent histologic evaluation, immunohistochemical analysis, and cytokine profiling via reverse transcriptase PCR assays. RESULTS: Kidney tissue from both groups had mononuclear inflammatory cell infiltration, tubular necrosis, dilated tubules, and glomerular damage. However, there was less fibrotic change and increased proliferation of renal tubular epithelial cells in the BM-MSC-treated dogs, compared with findings for the control dogs. Expressions of tumor necrosis factor-α and transforming growth factor-ß were lower in the BM-MSC-treated group, compared with findings for the control group. Laboratory data revealed no improvement in the renal function in BM-MSC-treated dogs. CONCLUSIONS AND CLINICAL RELEVANCE: Results of this study suggested that autologous BM-MSCs may accelerate renal regeneration after experimentally induced acute kidney injury in dogs.


Assuntos
Lesão Renal Aguda/induzido quimicamente , Células da Medula Óssea/fisiologia , Transplante de Células-Tronco Mesenquimais/veterinária , Células-Tronco Mesenquimais , Regeneração/fisiologia , Lesão Renal Aguda/terapia , Animais , Cisplatino/toxicidade , Cães , Taxa de Filtração Glomerular , Rim/fisiopatologia , Fator de Necrose Tumoral alfa
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