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1.
Fish Physiol Biochem ; 2019 Dec 06.
Artigo em Inglês | MEDLINE | ID: mdl-31811578

RESUMO

Light is an essential factor for organisms and affects the endocrine and stress regulation of fish in nature. However, sudden changes in light and dark conditions in artificial environments can negatively impact fish. In the present study, to evaluate the physiological and oxidative stress responses of goldfish (Carassius auratus) exposed to two different light conditions, sudden light changes and slowly dimming light changes for 24 h, we analyzed the mRNA expression and activity of stress indicators [corticotropin-releasing hormone (CRH) and pro-opiomelanocortin (POMC)], levels of plasma cortisol and glucose, mRNA expression of glucocorticoid receptor (GR), and activity of plasma oxidative stress indicators (superoxide dismutase and catalase). Consequently, the mRNA expressions and activities of CRH and POMC, plasma levels of cortisol and glucose, and mRNA expression of GR were found to be significantly increased during the light changes, particularly in the control group. Additionally, plasma levels of cortisol and glucose in the control group were significantly higher than those in the dimming group during the light changes. However, no significant differences in mRNA expression levels and activities of antioxidant enzymes both in the control and dimming groups were observed. These results indicate that dimming light induces less stress than sudden changes in light.

2.
Exp Ther Med ; 13(1): 146-154, 2017 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-28123483

RESUMO

Styphnolobium japonicum (L.) is utilized in Korean medicine for the treatment of various inflammatory diseases. The aim of the present study was to explore the effects of Fructus sophorae extract (FSE) isolated from the dried ripe fruit of S. japonicum (L.) on the development of type II collagen-induced arthritis (CIA) in BALB/c mice. The CIA mice were orally administered FSE or saline daily for 2 weeks. The incidence and severity of disease and the inflammatory response in the serum and the joint tissues were assessed. Macroscopic and histological investigation indicated that FSE protected against CIA development. FSE was associated with a significant reduction in the levels of total immunoglobulin G2a and proinflammatory cytokines and mediators in the serum. In addition, FSE suppressed the gene expression levels of proinflammatory cytokines and mediators, the mediator of osteoclastic bone remodeling, the receptor activator of nuclear factor κ-B ligand and matrix metalloproteinases in the joint tissues. The present results suggest that FSE may protect against inflammation and bone damage, and would be a valuable candidate for further investigation as a novel anti-arthritic agent.

3.
Mar Pollut Bull ; 113(1-2): 324-331, 2016 Dec 15.
Artigo em Inglês | MEDLINE | ID: mdl-27745743

RESUMO

In December 2007, >150km of the West coast of Korea were heavily polluted by crude oil leaked from the oil tanker Hebei Spirit, leading to mass mortality of bivalve mollusks on the intertidal areas. Two years after, mussels Mytilus galloprovincialis were collected from two impacted sites to investigate sub-lethal effects of the oil spill. Tissue content in polycyclic aromatic hydrocarbons (PAHs), hemocyte parameters, reproductive status and energetic reserves were analyzed. PAHs in tissues of mussels as well as hemocyte parameters were not different between impacted and control sites. Energetic reserves were altered in mussels from the impacted sites. Glycogen content remained low at polluted sites, whatever the season. Two years after the Hebei Spirit oil spill, mussels then presented altered energetic metabolism. Further investigations are thus warranted to monitor the sustainability of mussel populations on the oil spilled West coast of Korea.


Assuntos
Monitoramento Ambiental/métodos , Mytilus/efeitos dos fármacos , Poluição por Petróleo/efeitos adversos , Petróleo/toxicidade , Hidrocarbonetos Policíclicos Aromáticos/toxicidade , Poluentes Químicos da Água/toxicidade , Animais , Hemócitos/química , Mytilus/crescimento & desenvolvimento , Mytilus/metabolismo , Petróleo/análise , Poluição por Petróleo/análise , Hidrocarbonetos Policíclicos Aromáticos/análise , República da Coreia , Estações do Ano , Poluentes Químicos da Água/análise
4.
Mar Pollut Bull ; 108(1-2): 171-9, 2016 Jul 15.
Artigo em Inglês | MEDLINE | ID: mdl-27132991

RESUMO

Two years after the Hebei Spirit oil spill occurred off the west coast of Korea, we determined sub-lethal effects of the spilled oil on hemocyte parameters of Ruditapes philippinarum in the damaged areas. Clams in the spilled sites displayed unusually high proportion of granulocytes, which may result in higher phagocytosis capacity and reactive oxygen species production. Hemocytes in clams from the polluted sites also displayed less DNA damage and mortality than in the control site, possibly due to a faster phagocytosis of the impaired cells. Glycogen, the major energetic reserve, was depleted in clams from the spilled sites, potentially due to energetic consumption for maintenance of a large pool of granulocytes, detoxification processes and oxidative stress. Modified hemocyte parameters in clams in the spilled area, may reflect sub-lethal physiological stresses caused by the residual oils in the sediment, in conjunction with environmental modifications such as food availability and pathogens pattern.


Assuntos
Bivalves/efeitos dos fármacos , Monitoramento Ambiental/métodos , Hemócitos/efeitos dos fármacos , Poluição por Petróleo/análise , Poluentes Químicos da Água/toxicidade , Animais , Bivalves/metabolismo , Bivalves/fisiologia , Poluição por Petróleo/efeitos adversos , Fagocitose/efeitos dos fármacos , República da Coreia , Estresse Fisiológico/efeitos dos fármacos , Fatores de Tempo , Poluentes Químicos da Água/análise
5.
Braz J Microbiol ; 46(4): 991-1000, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-26691456

RESUMO

Fewer studies have assessed the outdoor cultivation of Spirulina maxima compared with S. platensis, although the protein content of S. maxima is higher than S. platensis. Spirulina growth medium requires an increased amount of NaHCO3, Na2CO3, and NaNO3, which increases the production cost. Therefore, the current study used a low-cost but high-efficiency biomass production medium (Medium M-19) after testing 33 different media. The medium depth of 25 cm (group A) was sub-divided into A1 (50% cover with a black curtain (PolyMax, 12 oz ultra-blackout), A2 (25% cover), and A3 (no cover). Similarly the medium depths of 30 and 35 cm were categorized as groups B (B1, B2, and B3) and C (C1, C2, and C3), respectively, and the effects of depth and surface light availability on growth and biomass production were assessed. The highest biomass production was 2.05 g L-1 in group A2, which was significantly higher (p < 0.05) than that in all other groups and sub-groups. Spirulina maxima died in B1 and C1 on the fifth day of culture. The biochemical composition of the biomass obtained from A2 cultures, including protein, carbohydrate, lipid, moisture, and ash, was 56.59%, 14.42%, 0.94%, 5.03%, and 23.02%, respectively. Therefore, S. maxima could be grown outdoors with the highest efficiency in urea-enriched medium at a 25-cm medium depth with 25% surface cover or uncovered.


Assuntos
Meios de Cultura/metabolismo , Técnicas de Cultura/métodos , Spirulina/crescimento & desenvolvimento , Spirulina/metabolismo , Ureia/metabolismo , Biomassa , Meios de Cultura/química , Técnicas de Cultura/instrumentação , Ureia/análise
6.
Braz. j. microbiol ; 46(4): 991-1000, Oct.-Dec. 2015. tab, graf
Artigo em Inglês | LILACS | ID: lil-769671

RESUMO

Fewer studies have assessed the outdoor cultivation of Spirulina maxima compared with S. platensis, although the protein content of S. maxima is higher than S. platensis. Spirulina growth medium requires an increased amount of NaHCO3, Na2CO3, and NaNO3, which increases the production cost. Therefore, the current study used a low-cost but high-efficiency biomass production medium (Medium M-19) after testing 33 different media. The medium depth of 25 cm (group A) was sub-divided into A1 (50% cover with a black curtain (PolyMax, 12 oz ultra-blackout), A2 (25% cover), and A3 (no cover). Similarly the medium depths of 30 and 35 cm were categorized as groups B (B1, B2, and B3) and C (C1, C2, and C3), respectively, and the effects of depth and surface light availability on growth and biomass production were assessed. The highest biomass production was 2.05 g L-1 in group A2, which was significantly higher (p < 0.05) than that in all other groups and sub-groups. Spirulina maxima died in B1 and C1 on the fifth day of culture. The biochemical composition of the biomass obtained from A2 cultures, including protein, carbohydrate, lipid, moisture, and ash, was 56.59%, 14.42%, 0.94%, 5.03%, and 23.02%, respectively. Therefore, S. maxima could be grown outdoors with the highest efficiency in urea-enriched medium at a 25-cm medium depth with 25% surface cover or uncovered.


Assuntos
Biomassa/análise , Biomassa/química , Biomassa/crescimento & desenvolvimento , Biomassa/instrumentação , Biomassa/metabolismo , Biomassa/métodos , Meios de Cultura/análise , Meios de Cultura/química , Meios de Cultura/crescimento & desenvolvimento , Meios de Cultura/instrumentação , Meios de Cultura/metabolismo , Meios de Cultura/métodos , Técnicas de Cultura/análise , Técnicas de Cultura/química , Técnicas de Cultura/crescimento & desenvolvimento , Técnicas de Cultura/instrumentação , Técnicas de Cultura/metabolismo , Técnicas de Cultura/métodos , Spirulina/análise , Spirulina/química , Spirulina/crescimento & desenvolvimento , Spirulina/instrumentação , Spirulina/metabolismo , Spirulina/métodos , Ureia/análise , Ureia/química , Ureia/crescimento & desenvolvimento , Ureia/instrumentação , Ureia/metabolismo , Ureia/métodos
7.
Mar Pollut Bull ; 90(1-2): 167-80, 2015 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-25467873

RESUMO

We examined trophic relationships in a newly established community 1.5 years after the Hebei Spirit oil spill on the west coast of Korea. Carbon and nitrogen stable isotope ratios in consumers and their potential food sources were compared between the oil-spill site and reference site, located 13.5 km from the oil-spill spot. The isotopic mixing model and a novel circular statistics rejected the influx of petrogenic carbon into the community and identified spatial consistencies such as the high contributions of microphytobenthos, food-chain length, and the isotopic niche of each feeding guild between sites. We suggested that high level of trophic plasticity and the prevalence of omnivory of consumers may promote the robustness of food web against the oil contamination. Furthermore, we highlighted the need of holistic approaches including different functional groups to quantify changes in the food web structure and assess the influence of different perturbations including oil spill.


Assuntos
Monitoramento Ambiental , Cadeia Alimentar , Poluição por Petróleo , Animais , Teorema de Bayes , Isótopos de Carbono/análise , Nitrogênio/análise , Isótopos de Nitrogênio/análise , República da Coreia
8.
Artigo em Inglês | MEDLINE | ID: mdl-25220187

RESUMO

This study aimed to test the effects of kisspeptin (Kiss) on somatic growth in the cinnamon clownfish Amphiprion melanopus. We investigated the effects of Kiss treatment on the growth by measuring the mRNA expressions of the growth hormone (GH), insulin-like growth hormone factor (IGF-I), somatolactin (SL), and melatonin receptor (MT). The expression levels of GH and SL of the pituitary gland and IGF-I of the liver increased after Kiss treatment (in vivo and in vitro). In addition, the MT mRNA expression increased in the pituitary gland and brain after Kiss treatment (in vivo and in vitro). These results support the hypothesis that Kiss directly regulates the somatic growth-related factors, such as GH, SL, and MT, and IGF-I in the cinnamon clownfish. Further, injection of Kiss resulted in significantly higher levels of plasma melatonin than that in the control. We, therefore, conclude that Kiss plays a role in modulating growth and artificially induced rapid growth in cinnamon clownfish.


Assuntos
Peptídeos e Proteínas de Sinalização Intercelular/metabolismo , Kisspeptinas/farmacologia , Perciformes/crescimento & desenvolvimento , Animais , Proteínas de Peixes/genética , Proteínas de Peixes/metabolismo , Regulação da Expressão Gênica/efeitos dos fármacos , Glicoproteínas/genética , Glicoproteínas/metabolismo , Hormônio do Crescimento/genética , Hormônio do Crescimento/metabolismo , Fator de Crescimento Insulin-Like I/genética , Fator de Crescimento Insulin-Like I/metabolismo , Fígado/metabolismo , Melatonina/genética , Melatonina/metabolismo , Perciformes/anatomia & histologia , Hipófise/citologia , Hipófise/efeitos dos fármacos , Hipófise/metabolismo , Hormônios Hipofisários/genética , Hormônios Hipofisários/metabolismo , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , Receptores de Melatonina/metabolismo , Fatores de Tempo
9.
Korean J Orthod ; 44(6): 342-9, 2014 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-25473650

RESUMO

Because of the potential morbidity and complications associated with surgical procedures, limiting the extent of orthognathic surgery is a desire for many orthodontic patients. An eighteen-year-old woman had a severe Class III malocclusion and required bi-maxillary surgery. By changing the patient's maxillary occlusal plane using orthodontic mini-implants, she was able to avoid the maxillary surgery; requiring only a mandibular setback surgery. To accurately predict the post-surgery outcome, we applied a new soft tissue prediction method. We were able to follow and report the long-term result of her combined orthodontic and orthognathic treatment. The changes to her occlusal plane continue to appear stable over 6 years later.

10.
Asian Pac J Trop Med ; 7(7): 531-5, 2014 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-25063282

RESUMO

OBJECTIVE: To find out and compare the in vitro antioxidant and tyrosinase inhibitory activities of two species of mangrove plants. METHODS: Mangrove samples were harvested at the shoreline on the island of Weno, Chuuk State in Micronesia. The phenol content, antioxidant activity (based on DPPH-free radical scavenging) and tyrosinase inhibitory activity in different tissues (leaves, barks and roots) of Rhizophora stylosa (R. stylosa) and Sonneratia alba (S. alba), collected from the island of Weno. RESULTS: Total phenol content ranged from 4.87 to 11.96 mg per g of freeze dried samples. The highest antioxidant activity was observed in R. stylosa bark (85.5%). The highest tyrosinase inhibitory activity was found in S. alba bark. Also, total phenol content and antioxidant activity were higher in methanol extracts than in aqueous extracts. CONCLUSIONS: Taken together, the results of this study proved that mangroves can be excellent sources of antioxidant compounds.


Assuntos
Antioxidantes , Lythraceae/química , Monofenol Mono-Oxigenase/antagonistas & inibidores , Extratos Vegetais , Rhizophoraceae/química , Antioxidantes/química , Antioxidantes/metabolismo , Compostos de Bifenilo , Inibidores Enzimáticos/química , Inibidores Enzimáticos/metabolismo , Micronésia , Monofenol Mono-Oxigenase/análise , Monofenol Mono-Oxigenase/metabolismo , Fenóis , Picratos , Extratos Vegetais/química , Extratos Vegetais/metabolismo
11.
Curr Microbiol ; 69(4): 445-50, 2014 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-24842302

RESUMO

A strain designated as S85(T) was isolated from a seaweed collected from coastal area of Chuuk State in Micronesia. The strain was gram-negative, rod-shaped, and non-motile and formed yellow colonies on the SWY agar (0.2 % yeast extract and 1.5 % agar in seawater) and Marine agar 2216. The strain grew at pH 5-9 (optimum, pH 8), at 15-40 °C (optimum, 25-28 °C), and with 1-9 % (w/v) NaCl (optimum, 3 %). The phylogenetic analysis based on 16S rRNA gene sequence showed that strain S85(T) was related to Lutibacter litoralis CL-TF09(T) and Maritimimonas rapanae A31(T) with 91.4 % and with 90.5 % similarity, respectively. The dominant fatty acids were iso-C15:0, iso-C15:0 3-OH and iso-C17:0 3-OH, C16:0 3-OH and summed feature 3 (C16:1 ω7c and/or iso-C15:0 2-OH). The major isoprenoid quinone was MK-6. The DNA G+C content of the type strain was 34.6 mol %. The major polar lipids were phosphatidylethanolamine, an unknown glycolipid and two unknown polar lipids. Based on this polyphasic taxonomic data, strain S85(T) stands for a novel species of a new genus, and we propose the name Ochrovirga pacifica gen. nov., sp. nov. The type strain of O. pacifica is S85(T) (=KCCM 90106 =JCM 18327(T)).


Assuntos
Flavobacteriaceae/isolamento & purificação , Água do Mar/microbiologia , Alga Marinha/microbiologia , Ágar/metabolismo , Composição de Bases , Ácidos Graxos/metabolismo , Flavobacteriaceae/classificação , Flavobacteriaceae/genética , Flavobacteriaceae/imunologia , Flavobacteriaceae/metabolismo , Micronésia , Dados de Sequência Molecular , Filogenia
12.
Curr Microbiol ; 67(6): 742-7, 2013 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-23907492

RESUMO

An aerobic, Gram-negative, coccoid to short rod-shaped and non-flagellated marine bacterial strain S354(T) was isolated from seawater of Micronesia. The strain was capable to degrade agar-forming slight depression into agar plate. Growth occurred at a temperature range of 12-44 °C, a pH range of 5-9, and a salinity range of 1-7 % (w/v) NaCl. Phylogenetic analyses based on 16S rRNA gene sequences suggested that S354(T) belongs to the family Flammeovirgaceae. The novel strain was most closely related to Limibacter armeniacum YM 11-185(T) with similarity of 92.5 %. The DNA G+C content was 43.8 mol%. The major fatty acids (>10 %) were iso-C15:0 and C16:1 ω5c. The predominant isoprenoid quinone was determined to be MK-7. Polar lipid profile of S354(T) consisted of phosphatidylethanolamine, unknown polar lipid, and unknown glycolipids. Based on the phenotypic, phylogenetic, biochemical, and physiological tests conducted in this study, S354(T) is proposed to represent a type strain of a novel genus and species. The 16S rRNA gene sequence of S354(T) is registered in GenBank under the accession number JQ639084. The type of strain Algivirga pacifica gen. nov., sp. nov. is S354(T) (=KCCM 90107(T)=JCM 18326(T)).


Assuntos
Ágar/metabolismo , Bacteroidetes/classificação , Bacteroidetes/isolamento & purificação , Água do Mar/microbiologia , Técnicas de Tipagem Bacteriana , Bacteroidetes/genética , Bacteroidetes/metabolismo , Composição de Bases , Análise por Conglomerados , DNA Bacteriano/química , DNA Bacteriano/genética , DNA Ribossômico/química , DNA Ribossômico/genética , Ácidos Graxos/análise , Concentração de Íons de Hidrogênio , Micronésia , Microscopia Eletrônica de Varredura , Dados de Sequência Molecular , Fosfolipídeos/análise , Filogenia , Quinonas/análise , RNA Ribossômico 16S/genética , Análise de Sequência de DNA , Cloreto de Sódio/metabolismo , Temperatura Ambiente
13.
Mar Pollut Bull ; 70(1-2): 189-96, 2013 May 15.
Artigo em Inglês | MEDLINE | ID: mdl-23522682

RESUMO

Petroleum hydrocarbon contamination and macrobenthos in the sandy tidal flats of Taean were monitored for 1 year to assess the impacts of Hebei Spirit oil on the macrobenthic community. A total of 207 macrobenthic fauna was collected, and the mean density and biomass of macrobenthic fauna continued to decrease until 12 months after the oil spill, but macrobenthic density at the most heavily affected sites increased by about twofold. In January 2008, the dominant species occurred at very low densities in strongly affected sites. The macrobenthic communities differed between oil-affected and unaffected sites. In particular, differences in community structure at Mallipo beach were larger than those at Shinduri. We suggest that long-term monitoring is needed to assess the specific effects of oil pollution on the sandy intertidal macrobenthic community.


Assuntos
Organismos Aquáticos/crescimento & desenvolvimento , Invertebrados/crescimento & desenvolvimento , Poluição por Petróleo/análise , Petróleo/análise , Poluentes Químicos da Água/análise , Animais , Organismos Aquáticos/classificação , Ecossistema , Monitoramento Ambiental , Sedimentos Geológicos/química , Invertebrados/classificação , República da Coreia , Água do Mar/química , Poluentes Químicos da Água/toxicidade
14.
Korean J Spine ; 10(4): 221-6, 2013 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-24891852

RESUMO

OBJECTIVE: The purpose of this study is to provide accurate understanding of clinical presentations and surgical outcomes as well as to identify the unique characteristics of lower lumbar osteoporotic compression fracture (OCF). METHODS: Clinical data were collected from 120 patients who had L3, L4 or L5 percutaneous vertebroplasty (PVP) performed from 2008 to 2012 at the single institute. L4 or L5 PVP patients were classified into group 1 and group 2 was for L3 PVP patients. Medical records were retrospectively investigated at 1 month after PVP. Long term follow-up results were obtained at a median value of 22 months after PVP. RESULTS: 75% of the patients in group 1 were not associated with traumatic events, 71% presenting with leg radiating symptoms and 46% requiring an additional decompressive surgery, more often than those in group 2. These differences are statistically significant (p<0.05). The short term medical record review demonstrated that only 73% of patients in group 1 were ameliorated with regard to back motion pain, whereas those in group 2 reported 87.7% rates of amelioration in identical category (p<0.05). The long term follow up confirmed a significantly worse outcome in group 1, with only 55.7% of patients reporting amelioration in their pain or functional status, but 71.7% rate of amelioration in group 2. CONCLUSION: The OCFs at the L4 or L5 level have different clinical characteristics from those at upper levels of the lumbar spine.

15.
Ecotoxicol Environ Saf ; 84: 18-24, 2012 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-22809708

RESUMO

Bioassays and biomarkers have been previously developed to assess the effects of heavy metal contaminants on the early life stages of the sea urchin. In this study, malformation in the early developmental processes was observed in sea urchin (Strongylocentrotus intermedius) larvae exposed to 10 ppm Ni for over 30 h. The most critical stage at which the triggering of nickel effects takes place is thought to be the blastula stage, which occurs after fertilization in larval development. To investigate the molecular-level responses of sea urchin exposed to heavy metal stress and to explore the differentially expressed genes that are induced or repressed by nickel, differential display polymerase chain reaction (DD-PCR) was used with sea urchin mRNAs. The malformation-related genes expressed in the early life stages of the sea urchin were cloned from larvae exposed to 10 ppm of nickel for 15 h, and accessed via DD-PCR. Sequence analysis results revealed that each of the genes evidenced high homology with EGF2, PCSK9, serine/threonine protein kinase, apolipophorin precursor protein, and MGC80921 protein/transcript variant 2. This result may prove useful in the development of novel biomarkers for the assessment of heavy metal stresses on sea urchin embryos.


Assuntos
Reação em Cadeia da Polimerase , Ouriços-do-Mar/efeitos dos fármacos , Ouriços-do-Mar/genética , Animais , Perfilação da Expressão Gênica , Regulação da Expressão Gênica no Desenvolvimento/efeitos dos fármacos , Larva , Metais Pesados/farmacologia , Níquel/farmacologia , Ouriços-do-Mar/embriologia , Homologia de Sequência de Aminoácidos
16.
J Bacteriol ; 194(5): 1260, 2012 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-22328757

RESUMO

In this study, we isolated xylan-degrading bacteria from a coastal lagoon of Micronesia and identified the bacteria as Marinobacterium stanieri S30. GSFLX 454 pyrosequencing and sequence analysis of the M. stanieri S30 genome generated 4,007 predicted open reading frames (ORFs) that could be candidate genes for producing enzymes with different catalytic functions.


Assuntos
Alteromonadaceae/genética , Alteromonadaceae/isolamento & purificação , DNA Bacteriano/química , DNA Bacteriano/genética , Genoma Bacteriano , Água do Mar/microbiologia , Alteromonadaceae/metabolismo , Micronésia , Dados de Sequência Molecular , Fases de Leitura Aberta , Análise de Sequência de DNA , Xilanos/metabolismo
17.
J Bacteriol ; 193(21): 6107, 2011 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-21994933

RESUMO

An agar-degrading marine bacterium identified as a novel member of the family Flavobacteriaceae (strain S85) was isolated from seawater in Micronesia. The sequenced strain S85 genome is composed of 3,384,629 bp in a circular chromosome, which includes 2,883 complete open reading frames.


Assuntos
DNA Bacteriano/química , DNA Bacteriano/genética , Flavobacteriaceae/genética , Genoma Bacteriano , Ágar/metabolismo , Cromossomos Bacterianos , Flavobacteriaceae/isolamento & purificação , Flavobacteriaceae/metabolismo , Micronésia , Dados de Sequência Molecular , Fases de Leitura Aberta , Água do Mar/microbiologia , Análise de Sequência de DNA
18.
J Bacteriol ; 193(19): 5538, 2011 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-21914866

RESUMO

We report here the annotated genome sequence of the marine bacterium Alteromonas sp. S89 and the identification of six genes coding for agar-degrading enzymes. The sequenced Alteromonas sp. S89 genome is composed of a 3,864,871-bp circular chromosome that includes 3,236 complete open reading frames.


Assuntos
Alteromonas/genética , Alteromonas/metabolismo , Genoma Bacteriano/genética , Glicosídeo Hidrolases/biossíntese , DNA Bacteriano/genética , Dados de Sequência Molecular , Fases de Leitura Aberta/genética
19.
J Bacteriol ; 193(19): 5557, 2011 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-21914876

RESUMO

We isolated Mesoflavibacter zeaxanthinifaciens S86 as xylanase-producing bacteria from seawater sampled in Micronesia. Analysis of the M. zeaxanthinifaciens genome revealed that it contains a single circular chromosome of 3,704,661 bp with 3,249 putative open reading frames.


Assuntos
Proteínas de Bactérias/metabolismo , Flavobacteriaceae/genética , Flavobacteriaceae/metabolismo , Genoma Bacteriano/genética , Xilosidases/metabolismo , Proteínas de Bactérias/genética , Cromossomos Bacterianos/genética , Dados de Sequência Molecular , Fases de Leitura Aberta/genética , Xilosidases/genética
20.
Fish Shellfish Immunol ; 28(1): 87-97, 2010 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-19822215

RESUMO

The disk abalone Haliotis discus discus and the spiny top shell Turbo cornutus are edible gastropod species of high economic value, mainly in Asia. Mortality outbreaks and variations in worldwide stock abundance have been reported and suggested to be associated, at least in part, with pathogenic infections. Ecology, biology and immunology of both species are currently not well documented. The characterisation of the immune systems of these species is necessary to further assess the responses of H. discus discus and T. cornutus to environmental, chemical and disease stresses. In the present study, we investigated the morphology and immune-related activities of hemocytes in both species using light microscopy and flow cytometry. Two types of hemocytes were identified in the disk abalone hemolymph, blast-like cells and hyalinocytes; whereas four main hemocyte types were distinguished in the spiny top shell, blast-like cells, type I and II hyalinocytes, and granulocytes. Flow cytometric analysis also revealed differences between cell types in immune-related activities. Three subsets of hemocytes, defined by differing lysosomal characteristics, were observed in the hemolymph of the spiny top shell, and only one in the disk abalone. Phagocytic activity was higher in H. discus discus hemocytes than in T. cornutus hemocytes, and the kinetics of PMA-stimulated oxidative activity was different between hemocytes of the disk abalone and the spiny top shell. Finally our results suggest for the first time a predominant mitochondrial origin of oxidative activity in gastropod hemocytes.


Assuntos
Gastrópodes/imunologia , Hemócitos/imunologia , Caramujos/imunologia , Animais , Ásia , Citometria de Fluxo , Hemócitos/classificação , Hemolinfa/imunologia , Imunidade/imunologia , Mitocôndrias/metabolismo , Oxirredução , Fagocitose/imunologia , Dinâmica Populacional
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