Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 46
Filtrar
Mais filtros










Tipo de estudo
Intervalo de ano de publicação
1.
Int J Polym Mater ; 63(7): 361-367, 2014.
Artigo em Inglês | MEDLINE | ID: mdl-25400302

RESUMO

Metabolic activity of the oral microbiota leads to acidification of the microenvironment and promotes demineralization of tooth structure at the margin of composite restorations. The pathogenic impact of the biofilm at the margin of the composite restoration could be reduced by engineering novel dentin adhesives that neutralize the acidic micro-environment. Integrating basic moieties into methacrylate derivatives has the potential to buffer against acid-induced degradation, and we are investigating basic monomers for this purpose. These monomers must be compatible with existing formulations, which are hydrophobic and marginally miscible with water. As such, cosolvent systems may be required to enable analysis of monomer function and chemical properties. Here we present an approach for examining the neutralizing capacity of basic methacrylate monomers in a water/ethanol co-solvent system using NMR spectroscopy. NMR is an excellent tool for monitoring the impact of co-solvent effects on pKa and buffering capacity of basic monomers because chemical shift is extremely sensitive to small changes that most other methods cannot detect. Because lactic acid (LA) is produced by oral bacteria and is prevalent in this microenvironment, LA was used to analyze the effectiveness of basic monomers to neutralize acid. The 13C chemical shift of the carbonyl in lactic acid was monitored as a function of ethanol and monomer concentration and each was correlated with pH to determine the functional buffering range. This study shows that the buffering capacity of even very poorly water-soluble monomers can be analyzed using NMR.

2.
J Biomed Mater Res B Appl Biomater ; 101(8): 1437-43, 2013 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-23744598

RESUMO

The aim of this study is to investigate the mechanical behavior of model methacrylate-based dentin adhesives under conditions that simulate the wet oral environment. A series of monotonic and creep experiments were performed on rectangular beam samples of dentin adhesive in three-point bending configuration under different moisture conditions. The monotonic test results show a significant effect of loading rate on the failure strength and the linear limit (yield point) of the stress-strain response. In addition, these tests show that the failure strength is low, and the failure occurs at a smaller deformation when the test is performed under continuously changing moisture conditions. The creep test results show that under constant moisture conditions, the model dentin adhesives can have a viscoelastic response under certain low loading levels. However, when the moisture conditions vary under the same low loading levels, the dentin adhesives have an anomalous creep response accompanied by large secondary creep and high strain accumulation.


Assuntos
Adesivos Dentinários/química , Metacrilatos/química , Osso e Ossos/metabolismo , Resinas Compostas , Colagem Dentária , Análise do Estresse Dentário , Dentina/química , Elasticidade , Dureza , Teste de Materiais , Cimentos de Resina , Estresse Mecânico , Propriedades de Superfície , Viscosidade
3.
Int J Polym Mater ; 61(2): 144-153, 2012.
Artigo em Inglês | MEDLINE | ID: mdl-22544985

RESUMO

The durability of dental resin depends on the stability of the polymer. The neutralizing capacity of a basic methacrylate monomer and its chemical stability were measured using nuclear magnetic resonance (NMR) spectroscopy. Lactic acid solution was titrated with 2-(dimethylamino)ethylmethacrylate (DMAEMA) or 2-hydroxyethylmethacrylate (HEMA) and its chemical shifts monitored. Addition of DMAEMA alters the chemical shift proportionally to pH neutralization, whereas HEMA has no impact. Chemical shifts were used to quantify both the change in pH and monomer stability. The results demonstrate that neutralization by basic monomer can be achieved and that this can be measured using an NMR assay.

4.
J Mater Sci Mater Med ; 23(5): 1157-72, 2012 May.
Artigo em Inglês | MEDLINE | ID: mdl-22430592

RESUMO

The diffusion of water into dentin adhesive polymers and leaching of unpolymerized monomer from the adhesive are linked to their mechanical softening and hydrolytic degradation. Therefore, diffusion coefficient data are critical for the mechanical design of these polymeric adhesives. In this study, diffusion coefficients of water and leachables were obtained for sixteen methacrylate-based crosslinked polymers using absorption experiments. The experimental mass change data was interpreted using numerical solution of the two-dimensional diffusion equations. The calculated diffusion coefficients varied from 1.05 × 10(-8) cm(2)/sec (co-monomer TMTMA) to 3.15 × 10(-8) cm(2)/sec (co-monomer T4EGDMA). Correlation of the diffusion coefficients with crosslink density and hydrophilicity showed an inverse trend (R(2) = 0.41). The correlation of diffusion coefficient with crosslink density and hydrophilicity are closer for molecules differing by simple repeat units (R(2) = 0.95). These differences in the trends reveal mechanisms of interaction of the diffusing water with the polymer structure.


Assuntos
Metacrilatos/química , Polímeros/farmacocinética , Água/metabolismo , Absorção , Reagentes para Ligações Cruzadas/farmacologia , Difusão , Teste de Materiais , Metacrilatos/farmacocinética , Modelos Biológicos , Modelos Teóricos , Concentração Osmolar , Polimerização , Polímeros/química , Cimentos de Resina/síntese química , Cimentos de Resina/química , Solubilidade , Água/química
5.
J Biomed Mater Res B Appl Biomater ; 100(4): 1086-92, 2012 May.
Artigo em Inglês | MEDLINE | ID: mdl-22331596

RESUMO

There have been reports of the sensitivity of our current dentin adhesives to excess moisture, for example, water-blisters in adhesives placed on over-wet surfaces, and phase separation with concomitant limited infiltration of the critical dimethacrylate component into the demineralized dentin matrix. To determine quantitatively the hydrophobic/hydrophilic components in the aqueous phase when exposed to over-wet environments, model adhesives were mixed with 16, 33, and 50 wt % water to yield well-separated phases. Based upon high-performance liquid chromatography coupled with photodiode array detection, it was found that the amounts of hydrophobic BisGMA and hydrophobic initiators are less than 0.1 wt % in the aqueous phase. The amount of these compounds decreased with an increase in the initial water content. The major components of the aqueous phase were hydroxyethyl methacrylate (HEMA) and water, and the HEMA content ranged from 18.3 to 14.7 wt %. Different BisGMA homologues and the relative content of these homologues in the aqueous phase have been identified; however, the amount of crosslinkable BisGMA was minimal and, thus, could not help in the formation of a crosslinked polymer network in the aqueous phase. Without the protection afforded by a strong crosslinked network, the poorly photoreactive compounds of this aqueous phase could be leached easily. These results suggest that adhesive formulations should be designed to include hydrophilic multimethacrylate monomers and water compatible initiators.


Assuntos
Dentina/química , Adesividade , Adesivos/análise , Materiais Biocompatíveis/química , Cromatografia Líquida de Alta Pressão/métodos , Adesivos Dentinários/análise , Desenho de Equipamento , Humanos , Interações Hidrofóbicas e Hidrofílicas , Metacrilatos/química , Metilmetacrilatos/química , Modelos Químicos , Espectrofotometria Ultravioleta/métodos , Água/química
6.
J Biomed Mater Res B Appl Biomater ; 100(2): 569-76, 2012 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-22114017

RESUMO

A new glycerol-based dimethacrylate monomer with an aromatic carboxylic acid, 2-((1,3-bis(methacryloyloxy)propan-2-yloxy)carbonyl)benzoic acid (BMPB), was synthesized, characterized, and proposed as a possible dental co-monomer for dentin adhesives. Dentin adhesives containing 2-hydroxyethyl methacrylate (HEMA) and 2,2-bis[4-(2-hydroxy-3-methacryloxypropoxy) phenyl]propane (BisGMA) in addition to BMPB were formulated with water at 0, 5, 10, and 15 wt % to simulate wet, oral conditions, and photo-polymerized. Adhesives were characterized with regard to viscosity, real-time photopolymerization behavior, dynamic mechanical analysis, and microscale 3D internal morphologies and compared with HEMA/BisGMA controls. When formulated under wet conditions, the experimental adhesives showed lower viscosities (0.04-0.07 Pa s) as compared to the control (0.09-0.12 Pa s). The experimental adhesives showed higher glass transition temperature (146-157°C), degree of conversion (78-89%), and rubbery moduli (33-36 MPa), and improved water miscibility (no voids) as compared to the controls (123-135°C, 67-71%, 15-26 MPa, and voids, respectively). The enhanced properties of these adhesives suggest that BMPB with simple, straightforward synthesis is a promising photocurable co-monomer for dental restorative materials.


Assuntos
Benzoatos/química , Cimentos Dentários/química , Cimentos Dentários/síntese química , Metacrilatos/química
7.
Braz. dent. sci ; 15(1): 4-18, 2012. ilus
Artigo em Inglês | LILACS, BBO - Odontologia | ID: lil-728072

RESUMO

Composite restorations have higher failure rates, more recurrent caries and increased frequency of replacement as compared to dental amalgam. Penetration of bacterial enzymes, oral fluids, and bacteria into the crevices between the tooth and composite undermines the restoration and leads to recurrent decay and failure. The gingival margin of composite restora tions is particularly vulnerable to decay and at this margin, the adhesive and its seal to dentin provides the primary barrier between the prepared tooth and the environment. The intent of this article is to examine physico-chemical factors that affect the integrity and durability of the adhesive/dentin interfacial bond; and to explore how these factors act synergistically with mechanical forces to undermine the composite restoration. The article will examine the various avenues that have been pursued to address these problems and it will explore how alterations in material chemistry could address the detrimental impact of physico-chemical stresses on the bond formed at the adhesive/dentin interface.


Restaurações em resina composta apresentam elevada taxa de falhas, recorrência de cárie e maior necessidade de troca quando comparadas às restaurações em amálgama. A penetração de enzimas bacterianas, fluidos orais e da própria bactéria nas fendas existentes entre o dente e o compósito, enfraquecem a restauração e levam à recorrência de cárie e falhas. A margem gengival das restaurações em resina composta é particularmente vulnerável à cárie e, nesta margem, o adesivo e o selamento dentinário funcionam como a primeira bareira entre o dente preparado e o ambiente oral. O objetivo deste artigo de revisão é examinar os fatores físico-químicos que afetam a integridade e a durabilidade da interface de adesão adesivo/dentina e explorar como esses fatores agem sinergicamente para minar a restauração de resina composta. A revisão irá examinar as diversas possibilidades para solucionar esses problemas, bem como explorar como alterações na química dos materiais poderiam solucionar o impacto negativo do estresse físico-químico na interface adesiva com a dentina.


Assuntos
Materiais Dentários
8.
Dent Mater ; 27(11): 1086-93, 2011 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-21816460

RESUMO

OBJECTIVES: The objective of this study was to investigate the influence of the chemical structure of methacrylate monomers used in dentin adhesives on degree of conversion (DC), water sorption, and dynamic mechanical properties. MATERIALS AND METHODS: Experimental adhesives containing 2,2-bis[4-(2-hydroxy-3-methacryloxypropoxy) phenyl]-propane (BisGMA), 2-hydroxyethyl methacrylate (HEMA), and co-monomer, 30/45/25 (w/w) were photo-polymerized. Ethyleneglycol dimethacrylate (EGDM), diethyleneglycol dimethacrylate (DEGDM), triethyleneglycol dimethacrylate (TEGDMA), 1,3-glycerol dimethacrylate (GDM), and glycerol trimethacrylate (GTM) were used as a co-monomer. The adhesives were characterized with regard to DC, water sorption, and dynamic mechanical analysis and compared to control adhesive [HEMA/BisGMA, 45/55 (w/w)]. RESULTS: DC and water sorption increased with an increase in the number of ethylene glycol units in the monomer. Experimental adhesive containing GDM showed significantly higher storage moduli (p<0.05) in both dry and wet samples than experimental adhesives containing EGDM or DEGDM. The rubbery moduli of adhesives containing GDM and GTM were found to be significantly greater (p<0.05) than that of the control. Adhesives containing GTM exhibited the widest tanδ curves, indicating the greatest structural heterogeneity. SIGNIFICANCE: The hydrophilicity, functionality and size of monomers in dentin adhesives affected the water sorption, solubility, crosslink density and heterogeneity of the polymer network. The experimental adhesives containing GDM and GTM showed higher rubbery moduli, indicating higher crosslink density accompanied by a decrease in the homogeneity of the polymer network structure.


Assuntos
Adesivos Dentinários/química , Etilenoglicol/química , Metacrilatos/química , Cimentos de Resina/química , Absorção , Bis-Fenol A-Glicidil Metacrilato/química , Interações Hidrofóbicas e Hidrofílicas , Teste de Materiais , Fenômenos Mecânicos , Estrutura Molecular , Fotoiniciadores Dentários , Polietilenoglicóis/química , Polimerização , Ácidos Polimetacrílicos/química , Poliuretanos/química , Solubilidade , Relação Estrutura-Atividade , Água
9.
Dent Mater ; 27(9): e187-95, 2011 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-21700326

RESUMO

OBJECTIVES: The objective of this work was to develop a methodology for the prediction of fatigue life of the dentin-adhesive (d-a) interface. METHODS: At the micro-scale, the d-a interface is composed of dissimilar material components. Under global loading, these components experience different local stress amplitudes. The overall fatigue life of the d-a interface is, therefore, determined by the material component that has the shortest fatigue life under local stresses. Multiple 3d finite element (FE) models were developed to determine the stress distribution within the d-a interface by considering variations in micro-scale geometry, material composition and boundary conditions. The results from these models were analyzed to obtain the local stress concentrations within each d-a interface component. By combining the local stress concentrations and experimentally determined stress versus number of cycle to failure (S-N) curves for the different material components, the overall fatigue life of the d-a interface was predicted. RESULTS: The fatigue life was found to be a function of the applied loading amplitude, boundary conditions, microstructure and the mechanical properties of the material components of the d-a interface. In addition, it was found that the overall fatigue life of the d-a interface is not determined by the weakest material component. In many cases, the overall fatigue life was determined by the adhesive although exposed collagen was the weakest material component. Comparison of the predicted results with experimental data from the literature showed both qualitative and quantitative agreement. SIGNIFICANCE: The methodology developed for fatigue life prediction can provide insight into the mechanisms that control degradation of the bond formed at the d-a interface.


Assuntos
Colagem Dentária , Análise do Estresse Dentário/métodos , Adesivos Dentinários , Adesivos , Colágeno/química , Resinas Compostas , Dentina/anatomia & histologia , Módulo de Elasticidade , Análise de Elementos Finitos , Humanos , Modelos Estruturais , Cimentos de Resina , Estresse Mecânico
10.
J Biomed Mater Res B Appl Biomater ; 95(2): 283-90, 2010 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-20848661

RESUMO

The objective of the current study is to characterize the viscoelastic and fatigue properties of model methacrylate-based dentin adhesives under dry and wet conditions. Static, creep, and fatigue tests were performed on cylindrical samples in a 3-point bending clamp. Static results showed that the apparent elastic modulus of the model adhesive varied from 2.56 to 3.53 GPa in the dry condition, and from 1.04 to 1.62 GPa in the wet condition, depending upon the rate of loading. Significant differences were also found for the creep behavior of the model adhesive under dry and wet conditions. A linear viscoelastic model was developed by fitting the adhesive creep behavior. The developed model with 5 Kelvin Voigt elements predicted the apparent elastic moduli measured in the static tests. The model was then utilized to interpret the fatigue test results. It was found that the failure under cyclic loading can be due to creep or fatigue, which has implications for the failure criterion that are applied for these types of tests. Finally, it was found that the adhesive samples tested under dry conditions were more durable than those tested under wet conditions.


Assuntos
Cimentos Dentários , Dentina , Teste de Materiais , Metacrilatos , Viscosidade , Modelos Teóricos
11.
Ann Biomed Eng ; 38(6): 1989-2003, 2010 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-20195761

RESUMO

Results from clinical studies suggest that more than half of the 166 million dental restorations that were placed in the United States in 2005 were replacements for failed restorations. This emphasis on replacement therapy is expected to grow as dentists use composite as opposed to dental amalgam to restore moderate to large posterior lesions. Composite restorations have higher failure rates, more recurrent caries, and increased frequency of replacement as compared to amalgam. Penetration of bacterial enzymes, oral fluids, and bacteria into the crevices between the tooth and composite undermines the restoration and leads to recurrent decay and premature failure. Under in vivo conditions the bond formed at the adhesive/dentin interface can be the first defense against these noxious, damaging substances. The intent of this article is to review structural aspects of the clinical substrate that impact bond formation at the adhesive/dentin interface; to examine physico-chemical factors that affect the integrity and durability of the adhesive/dentin interfacial bond; and to explore how these factors act synergistically with mechanical forces to undermine the composite restoration. The article will examine the various avenues that have been pursued to address these problems and it will explore how alterations in material chemistry could address the detrimental impact of physico-chemical stresses on the bond formed at the adhesive/dentin interface.


Assuntos
Resinas Compostas/química , Cimentos Dentários/química , Dentina/química , Adesividade , Animais , Humanos , Teste de Materiais , Propriedades de Superfície , Resistência à Tração
12.
Int J Mol Med ; 25(1): 113-20, 2010 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-19956909

RESUMO

In the present study, single-stranded large circular (LC)-sense molecules were utilized as probes for DNA microarrays and showed stronger binding signals than those of PCR-amplified cDNA probes. A microarray experiment using 284 LC-sense DNA probes found 6 upregulated and 7 downregulated genes in A549 cells as compared to WI38VA13 cells. Repeated experiments showed largely consistent results, and microarray data strongly correlated with data acquired from quantitative real-time RT-PCR. A large array comprising 5,079 LC-sense DNA was prepared, and analysis of the mean differential expression from dye-swap experiments revealed 332 upregulated and 509 downregulated genes in A549 cells compared to WI38VA13 cells. Subsequent functional analysis using an LC-antisense library of overexpressed genes identified 28 genes involved in A549 cell growth. These experiments demonstrated the proper features of LC-sense molecules as probe DNA for microarray and the potential utility of the combination of LC-sense and -antisense libraries for an effective functional validation of genes.


Assuntos
DNA Antissenso/genética , DNA de Cadeia Simples/genética , Perfilação da Expressão Gênica/métodos , Neoplasias Pulmonares/genética , Análise de Sequência com Séries de Oligonucleotídeos/métodos , Linhagem Celular Transformada , Linhagem Celular Tumoral , Proliferação de Células , DNA Complementar/genética , Perfilação da Expressão Gênica/economia , Regulação Neoplásica da Expressão Gênica , Humanos , Análise de Sequência com Séries de Oligonucleotídeos/economia , Reprodutibilidade dos Testes , Reação em Cadeia da Polimerase Via Transcriptase Reversa
13.
J Biomed Mater Res A ; 93(4): 1245-51, 2010 Jun 15.
Artigo em Inglês | MEDLINE | ID: mdl-19827107

RESUMO

The selection of an appropriate photoinitiator system is critical for efficient polymerization of dental resins with satisfactory mechanical and physical properties. The purpose of this study was to evaluate the influence of adding an iodonium salt to two-component photoinitiator systems. Four photoinitiator systems were included in a model bisGMA/HEMA resin and used to prepare samples at different water contents; the dynamic mechanical properties and the final degree of conversion of the samples were then characterized. Addition of the iodonium salt to the two-component photoinitiator systems increased the final degree of conversion, glass transition temperature, rubbery modulus, and crosslink density. The photoinitiator system containing ethyl-4-(dimethylamino) benzoate as a coinitiator and the iodonium salt exhibited the highest rubbery modulus. The enhanced properties in the presence of the iodonium salt can be attributed to the production of an active phenyl radical with regeneration of the original camphorquinone, which may increase the compatibility between monomers and initiators, especially in the presence of water. The results support the hypothesis that a photoinitiator system containing an iodonium salt can increase both mechanical properties and final conversion of model resin polymerized in the presence of water.


Assuntos
Bis-Fenol A-Glicidil Metacrilato/química , Luz , Metacrilatos/química , Resinas Sintéticas/química , Cânfora/análogos & derivados , Cânfora/química , Reagentes para Ligações Cruzadas/farmacologia , Vidro , Fotoquímica/métodos , Polímeros/química , Análise Espectral Raman/métodos , Estresse Mecânico , Temperatura , Água/química
14.
Dent Mater ; 25(12): 1569-75, 2009 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-19709724

RESUMO

OBJECTIVES: Our previous study showed the synthesis and characterization of a novel urethane-linked trimethacrylate monomer for use as a co-monomer in dentin adhesives. The objective of this work was to further investigate the performance of dentin adhesives containing a new monomer, with particular emphasis on the water sorption and viscoelastic behavior of the crosslinked networks. MATERIALS AND METHODS: Dentin adhesives contained 2,2-bis[4-(2-hydroxy-3-methacryloxypropoxy) phenyl]-propane (BisGMA), 2-hydroxyethyl methacrylate (HEMA), and a new multifunctional methacrylate with urethane-linked groups-1,1,1-tri-[4-(methacryloxyethylaminocarbonyloxy)-phenyl]ethane (MPE) and were photo-polymerized in the presence or absence of water. Adhesives were characterized with regard to degree of conversion (DC), viscosity, water sorption/solubility, and dynamic mechanical analysis (DMA) and compared with BisGMA/HEMA controls. RESULTS: The experimental adhesives exhibited DC and solubility comparable to that of the control, regardless of the presence or absence of water. All the experimental adhesives tested showed less water sorption, lower tandelta peak heights, and higher rubbery modulus than the control. SIGNIFICANCE: Dentin adhesives containing a new multifunctional methacrylate showed better dynamic thermomechanical properties and water sorption relative to controls, without compromising DC and solubility. Thus, MPE, when included as a component of methacrylate dentin adhesives, may provide enhanced durability in the moist environment of the mouth.


Assuntos
Resinas Compostas/química , Adesivos Dentinários/química , Metacrilatos/química , Poliuretanos/química , Água/química , Absorção , Adsorção , Algoritmos , Bis-Fenol A-Glicidil Metacrilato/química , Módulo de Elasticidade , Elasticidade , Humanos , Teste de Materiais , Fenômenos Mecânicos , Polímeros/química , Solubilidade , Análise Espectral Raman , Temperatura , Fatores de Tempo , Temperatura de Transição , Viscosidade
15.
J Biomed Mater Res B Appl Biomater ; 91(2): 562-71, 2009 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-19582843

RESUMO

A new trimethacrylate monomer with urethane-linked groups, 1,1,1-tri-[4-(methacryloxyethylamino-carbonyloxy)-phenyl]ethane (MPE), was synthesized, characterized, and used as a comonomer in dentin adhesives. Dentin adhesives containing 2-hydroxyethyl methacrylate (HEMA, 45% w/w) and 2,2-bis[4(2-hydroxy-3-methacryloyloxy-propyloxy)-phenyl] propane (BisGMA, 30% w/w) in addition to MPE (25% w/w) were formulated with H(2)O at 0 (MPE0), 8 (MPE8), and 16 wt % water (MPE16) to simulate the wet demineralized dentin matrix and compared with controls [HEMA /BisGMA, 45/55 w/w, at 0 (C0), 8 (C8), and 16 wt % water (C16)]. The new adhesive showed a degree of double bond conversion and mechanical properties comparable with control, with good penetration into the dentin surface and a uniform adhesive/dentin interface. On exposure to porcine liver esterase, the net cumulative methacrylic acid (MAA) released from the new adhesives was dramatically (p < 0.05) decreased relative to the control, suggesting that the new monomer improves esterase resistance.


Assuntos
Adesivos/química , Materiais Dentários/química , Dentina/química , Metilmetacrilatos/química , Uretana/química , Implantes Absorvíveis , Resinas Acrílicas/química , Adesividade , Enzimas/química , Esterases/química , Hidrólise , Espectroscopia de Ressonância Magnética , Teste de Materiais , Microscopia Eletrônica de Varredura , Coloração e Rotulagem , Estresse Mecânico
16.
Bioorg Med Chem Lett ; 19(11): 2986-9, 2009 Jun 01.
Artigo em Inglês | MEDLINE | ID: mdl-19409781

RESUMO

The new cholesterol-based cationic lipids B, C, and D with an ether linked spacer were synthesized by using aminopropyl chain extension with acrylonitrile. The cholesterol-based cationic lipid A with carbamoyl linkage were also synthesized in order to compare the difference in transfection efficiency of the two linkage types. To this end, GFP expression of these cationic lipids was confirmed respectively.


Assuntos
Cátions/química , Colesterol/química , Lipídeos/síntese química , Transfecção , Linhagem Celular Tumoral , Humanos , Lipídeos/química , Lipídeos/farmacologia , Lipossomos/química
17.
J Biomed Mater Res B Appl Biomater ; 91(1): 61-70, 2009 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-19358261

RESUMO

A study of the dynamic mechanical properties and the enzymatic degradation of new dentin adhesives containing a multifunctional methacrylate are described. Adhesives contained 2-hydroxyethyl methacrylate, 2,2-bis[4-(2-hydroxy-3-methacryloxypropoxy) phenyl]-propane, and a new multifunctional methacrylate with a branched side chain-trimethylolpropane mono allyl ether dimethacrylate (TMPEDMA). Adhesives were photopolymerized in the presence of 0, 8, and 16 wt % water to simulate wet bonding conditions in the mouth and compared with control adhesives. The degree of conversion as a function of irradiation time was comparable for experimental and control adhesives. In dynamic mechanical analysis, broad tan delta peaks were obtained for all samples, indicating that the polymerized networks are heterogeneous; comparison of the full-width-at-half-maximum values obtained from the tan delta curves indicated increased heterogeneity for samples cured in the presence of water and/or containing TMPEDMA. The experimental adhesive showed higher T(g) and higher rubbery modulus indicating increased crosslink density when compared with the control. The improvement in esterase resistance afforded by adhesives containing the TMPEDMA is greater when this material is photopolymerized in the presence of water, suggesting better performance in the moist environment of the mouth. The improved esterase resistance of the new adhesive could be explained in terms of the densely crosslinked network structure and/or the steric hindrance of branched alkyl side chains.


Assuntos
Adesivos Dentinários , Dentina/metabolismo , Esterases/metabolismo , Metacrilatos , Materiais Biocompatíveis/química , Materiais Biocompatíveis/metabolismo , Adesivos Dentinários/química , Adesivos Dentinários/metabolismo , Teste de Materiais , Metacrilatos/química , Metacrilatos/metabolismo , Estrutura Molecular
18.
Toxicol In Vitro ; 23(2): 281-7, 2009 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-19135517

RESUMO

Sanguinarine is a plant-derived benzophenanthridine alkaloid and has been shown to possess anti-tumor activities against various cancer cells. In this study, we investigated whether sanguinarine induces apoptosis in A549 human lung cancer cells. Treatment of A549 cells with sanguinarine induced apoptosis in a dose- and time-dependent manner. Treatment with sanguinarine led to activation of caspases and MAPKs as well as increased MKP-1 expression. Importantly, pretreatment with z-VAD-fmk, a pan caspase inhibitor suppressed the sanguinarine-induced apoptosis in A549 cells. Moreover, pretreatment with NAC, a sulfhydryl group-containing reducing agent strongly suppressed the apoptotic response and caspase activation to sanguinarine. However, the sanguinarine-mediated cytotoxicity in A549 cells was not protected by pharmacological inhibition of MAPKs or MKP-1 siRNA-mediated knockdown of MKP-1. These results collectively suggest that sanguinarine induces apoptosis in A549 cells through cellular glutathione depletion and the subsequent caspase activation.


Assuntos
Antineoplásicos Fitogênicos/farmacologia , Apoptose/efeitos dos fármacos , Benzofenantridinas/farmacologia , Fosfatase 1 de Especificidade Dupla/metabolismo , Glutationa/metabolismo , Isoquinolinas/farmacologia , Neoplasias Pulmonares/tratamento farmacológico , Quinases de Proteína Quinase Ativadas por Mitógeno/metabolismo , Clorometilcetonas de Aminoácidos/farmacologia , Inibidores de Caspase , Caspases/metabolismo , Linhagem Celular Tumoral , Sobrevivência Celular/efeitos dos fármacos , Relação Dose-Resposta a Droga , Ensaios de Seleção de Medicamentos Antitumorais , Fosfatase 1 de Especificidade Dupla/genética , Ativação Enzimática/efeitos dos fármacos , Inibidores Enzimáticos , Inativação Gênica , Humanos , Neoplasias Pulmonares/metabolismo , Neoplasias Pulmonares/patologia , Quinases de Proteína Quinase Ativadas por Mitógeno/antagonistas & inibidores , Fármacos Neuroprotetores/farmacologia , RNA Interferente Pequeno/genética , RNA Interferente Pequeno/metabolismo
19.
J Biomed Mater Res B Appl Biomater ; 88(2): 394-401, 2009 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-18395826

RESUMO

Dentin adhesives may undergo phase separation when bonding to wet demineralized dentin. We hypothesized that adhesives exhibiting phase separation will experience enhanced biodegradation of methacrylate ester groups. The objective of this project was to study the effect of enzyme-exposure on the release of methacrylic acid (MAA) and 2-hydroxyethyl methacrylate (HEMA) from adhesives formulated under conditions simulating wet bonding. HEMA/bisGMA(2,2-bis[4(2-hydroxy-3-methacryloyloxy-propyloxy)-phenyl] propane), 45/55 w/w ratio, was formulated with different water content: 0 Wt % (A00), 8 wt % (A08), and 16 wt % (A16). After a three day prewash, adhesive discs were incubated with/without porcine liver esterase (PLE) in phosphate buffer (PB, pH 7.4) at 37 degrees C for 8 days. Supernatants were collected daily and analyzed for MAA and HEMA by HPLC. For all formulations, daily MAA release in the presence of PLE was increased compared to MAA release in PB. HEMA release in the presence of PLE was not detected while HEMA release was consistently measured in PB. A08 and A16 released significantly larger amounts of HEMA compared to A00. Analysis of the cumulative release of analytes showed that the leachables in PLE was significantly increased (p < 0.05) as compared with that released in PB indicating that MAA release was not only formed from unreacted monomers but from pendant groups in the polymer network. However, the levels of analytes HEMA in PB or MAA in PLE were increased in A08 and A16 as compared with A00, which suggests that there could be a greater loss of material in HEMA/bisGMA adhesives that experience phase separation under wet bonding conditions.


Assuntos
Esterases/metabolismo , Metacrilatos/química , Metacrilatos/metabolismo , Água/química , Adesividade , Animais , Microscopia Eletrônica de Varredura , Suínos
20.
Dent Mater ; 25(4): 452-8, 2009 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-19027937

RESUMO

OBJECTIVES: Our previous study showed poor mechanical durability and nano-sized heterogeneities in cross-linked dentin adhesives cured in the presence of water. To further explore the relationship between nano-scale heterogeneities and the long-term mechanical properties of dentin adhesives, the properties of model dentin adhesives polymerized using hydrophilic photoinitiators were compared with those of adhesives polymerized using hydrophobic camphorquinone-based photoinitiators. METHODS: The model adhesives consisted of HEMA and bisGMA with a mass ratio of 45/55 and were photopolymerized in the presence of 8.3 mass% water. The photo-polymerization of the model adhesives during irradiation was monitored in situ using a Perkin-Elmer Spectrum One FTIR in the ATR sampling mode. The tensile properties were determined for all samples after dry storage at room temperature, or after aqueous storage in distilled deionized water. RESULTS: There was a continuous decline of mechanical properties for the specimens cured in the presence of water during 3 months aqueous storage, especially for the specimens that contained hydrophobic photoinitiators. The multi-component systems containing hydrophilic photoinitiators were shown to produce superior model dental adhesives when these materials are cured in the presence of water. SIGNIFICANCE: Designing initiator systems to perform in this heterogeneous environment may improve the mechanical performance of dentin adhesives, as the results presented here indicate.


Assuntos
Adesivos Dentinários , Cura Luminosa de Adesivos Dentários , Processos Fotoquímicos/efeitos dos fármacos , Cimentos de Resina , Adesividade , Compostos de Bifenilo/farmacologia , Bis-Fenol A-Glicidil Metacrilato , Análise do Estresse Dentário , Adesivos Dentinários/química , Módulo de Elasticidade , Dureza , Interações Hidrofóbicas e Hidrofílicas , Teste de Materiais , Metacrilatos/farmacologia , Nanopartículas , Oniocompostos/farmacologia , Transição de Fase , Cimentos de Resina/química , Espectroscopia de Infravermelho com Transformada de Fourier , Resistência à Tração , Terpenos/farmacologia , Água
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...