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1.
Plant J ; 2020 Mar 13.
Artigo em Inglês | MEDLINE | ID: mdl-32168401

RESUMO

The fungal genus Cochliobolus describes necrotrophic pathogens that give rise to significant losses on rice, wheat, and maize. Revealing plant mechanisms of non-host resistance (NHR) against Cochliobolus will help to uncover strategies that can be exploited in engineered cereals. Therefore, we developed a heterogeneous pathosystem and studied the ability of Cochliobolus to infect dicotyledons. We report here that C. miyabeanus and C. heterostrophus infect Arabidopsis accessions and produce functional conidia, thereby demonstrating the ability to accept Brassica spp. as host plants. Some ecotypes exhibited a high susceptibility, whereas others hindered the necrotrophic disease progression of the Cochliobolus strains. Natural variation in NHR among the tested Arabidopsis accessions can advance the identification of genetic loci that prime the plant's defence repertoire. We found that applied phytotoxin-containing conidial fluid extracts of C. miyabeanus caused necrotic lesions on rice leaves but provoked only minor irritations on Arabidopsis. This result implies that C. miyabeanus phytotoxins are insufficiently adapted to promote dicot colonization, which corresponds to a retarded infection progression. Previous studies on rice demonstrated that ethylene (ET) promotes C. miyabeanus infection, whereas salicylic acid (SA) and jasmonic acid (JA) exert a minor function. However, in Arabidopsis, we revealed that the genetic disruption of the ET and JA signalling pathways compromises basal resistance against Cochliobolus, whereas SA biosynthesis mutants showed a reduced susceptibility. Our results refer to the synergistic action of ET/JA and indicate distinct defence systems between Arabidopsis and rice to confine Cochliobolus propagation. Moreover, this heterogeneous pathosystem may help to reveal mechanisms of NHR and associated defensive genes against Cochliobolus infection.

2.
J Urol ; : 101097JU0000000000000794, 2020 Jan 31.
Artigo em Inglês | MEDLINE | ID: mdl-32003614

RESUMO

PURPOSE: The clinicopathological features and treatment outcomes of plasmacytoid variant (PV)-urothelial carcinoma of the bladder (UCB) have not been fully understood. We aimed to evaluate the clinicopathologic characteristics and survival outcomes of PV-UCB as compared to conventional UCB (C-UCB). METHODS: A systematic review was performed following the PRISMA guideline. PubMed/Medline, Embase, and Cochrane Library were searched up to June 2019. The differences in the clinicopathological features (≥stage pT3, lymph node metastasis, ureteral margin-positive, and perivesical soft tissue margin-positive status) and survival outcomes [overall mortality (OM) and cancer-specific mortality (CSM)] between PV-UCB and C-UCB were compared. The GRADE approach was used for rating the certainty of evidence. RESULTS: A total of 8 studies were included. Patients with PV-UCB had a higher frequency of ≥stage pT3 (odds ratio [OR], 3.84; 95% confidence interval [CI], 1.63-9.03; p=0.002) and risk of lymph node metastasis (OR, 2.58; 95% CI, 1.15-5.76; p=0.02), ureteral margin-positive (OR 12.18; 95% CI, 4.62-32.13; p<0.00001), and perivesical soft tissue margin-positive (OR 12.31; 95% CI, 5.15-29.41; p<0.00001) status after radical cystectomy than those with C-UCB. Although there was no difference in CSM (hazard ratio [HR], 1.40; 95% CI, 0.82-2.40; p=0.22) between PV-UCB and C-UCB, PV-UCB had worse survival outcomes (OM) than C-UCB approaching the borderline of significance (HR, 1.62; 95% CI, 0.98-2.68; p=0.06) when adjusted for other clinicopathological characteristics. CONCLUSIONS: PV-UCB was strongly associated with adverse clinicopathological features and worse OM compared to C-UCB after adjusting other clinicopathological parameters, and PV histology of UCB is an independent prognostic factor for overall survival.

3.
Acta Biomater ; 101: 273-284, 2020 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-31707084

RESUMO

Epiphora is the overflow of tears typically caused by obstruction or occlusion of the nasolacrimal duct. More attention is required to address this global health issue owing to the increase in air pollution. Implantation of a silicone stent is the preferred treatment for epiphora; however, introducing a silicone stent into a narrow duct with complex geometry is challenging as it requires guidance by a sharp metal needle. Additionally, silicone can cause adverse reactions such as biofilm formation and tear flow resistance due to its extreme hydrophobicity. To overcome these problems, in this study we developed a new type of biocompatible shape memory polymer (SMP) stent with elasticity capacity for self-expansion. First, SMPs in the form of x%poly(ε-caprolactone)-co-y%poly(glycidyl methacrylate) (x%PCL-y%PGMA) were synthesized via ring opening polymerization by varying the molar ratio of PCL (x%) and PGMA (y%). Second, the shape memory and mechanical properties were tuned by controlling the crosslinking degree and concentration of x%PCL-y%PGMA solution to produce a test type of SMP stent. Lastly, this 94%PCL-06%PGMA stent exhibited more standout critical functions in a series of in vitro and in vivo experiments such as a cell growth-supporting level of biocompatibility with nasal epithelial cells without significant inflammatory responses, better resistance to biofilm formation, and more efficient capacity to drain tear than the silicone control. Overall, 94%PCL-06%PGMA can be suggested as a superior alternative to the currently used materials for nasolacrimal stents. STATEMENT OF SIGNIFICANCE: Silicone intubation (stenting) has been widely used to treat nasolacrimal duct obstruction, however, it can cause adverse clinical effects such as bacterial infection; presents procedural challenges because of the curved nasolacrimal duct structure; and shows poor drainage efficiency stemming from the highly hydrophobic nature of silicone. In this work, we describe an innovative shape memory polymer (SMP) as a superior alternative to conventional silicone-based materials for nasolacrimal duct intubation. We demonstrate the clear advantages of the SMP over conventional silicone, including a much higher drainage capacity and superior resistance to bacterial infection.

4.
Neuropharmacology ; 166: 107928, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-31887307

RESUMO

Parkinson's disease (PD) is characterized by the selective loss of nigrostriatal dopamine neurons associated with microglial activation. Inhibition of the inflammatory response elicited by activated microglia could be an effective strategy to alleviate the progression of PD. Here, we synthesized 2-(5-(4-chlorophenyl)-1-(2,4-dichlorophenyl)-4-methyl-1H-pyrazol-3-yl)-N-(2-hydroxyethyl)-2-oxoacetamide (CDMPO) and studied its protective anti-inflammatory mechanisms following lipopolysaccharide (LPS)-induced neuroinflammation in vitro and 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced neurotoxicity in vivo. CDMPO and its parent compound, rimonabant, significantly attenuated nitric oxide (NO) production in LPS-stimulated primary microglia and BV2 cells. Furthermore, CDMPO significantly inhibited the release of proinflammatory cytokines and prostaglandin E2 (PGE2) by activated BV2 cells, also suppressed expression of inducible nitric oxide synthase (iNOS), and cyclooxygenase-2 (COX-2). Mechanistically, CDMPO attenuated LPS-induced activation of nuclear factor-kappa B (NF-κB), inhibitor of kappa B alpha (IκBα), and p38 phosphorylation in BV2 cells. MPTP intoxication of mice results in glial activation, tyrosine hydroxylase (TH) depletion, and significant behavioral deficits. Prophylactic treatment with CDMPO decreased proinflammatory molecules via NF-κB and p38 mitogen-activated protein kinase signaling, resulting in protection of dopaminergic neurons and improved behavioral impairments. These results suggest that CDMPO is a promising neuroprotective agent for the prevention and treatment of microglia-mediated neuroinflammatory conditions and may be useful for behavioral improvement in PD phenotype.

5.
Front Psychol ; 10: 1992, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31572254

RESUMO

Background: The purpose of this study was to examine the psychometric properties of the Rosenberg Self-Esteem Scale for individuals with intellectual disabilities (ID) using the Rasch model and to determine whether the scale is valid and reliable for use with this population. Methods: Rasch analysis was carried out on data from 223 respondents to the 8th Panel Survey on Employment for the Disabled conducted by the Korea Employment Agency for the Disabled. The validity and reliability of scale items were verified through analyses of item fit, item difficulties, the rating scale, and separation indices. Results: Item infit mean square values were found to range between 0.71 and 1.25, and item outfit mean square values between 0.71 and 1.26. Additionally, item difficulties were appropriate; Item 4 was the most difficult item, while Item 10 was the easiest item. The 4-point rating scale was appropriate, and the separation indices were at an acceptable level. Conclusion: Based on these results, the validity and reliability of the Rosenberg Self-Esteem Scale for use with individuals with ID were verified. Thus, this scale can be regarded as a useful tool for evaluating the level of self-esteem of individuals with ID.

6.
Artigo em Inglês | MEDLINE | ID: mdl-31619017

RESUMO

Sympathetic overactivity is associated with hyperhidrosis and cardiovascular diseases. Endoscopic thoracic sympathectomy (ETS) is a treatment for hyperhidrosis. We aimed to compare the risk for cardiovascular events between individuals with and without hyperhidrosis and investigate the effects of ETS on cardiovascular outcomes. We conducted a nationwide population-based cohort study using data acquired from the Korean Health Insurance Review and Assessment Service. Subjects newly diagnosed with hyperhidrosis in 2010 were identified and divided into two groups according to whether or not they underwent ETS. Propensity scores were calculated using a logistic regression model to match hyperhidrosis patients with control subjects. Combined cardiovascular events were defined as stroke and ischemic heart diseases. Subjects were followed up until the first cardiovascular event or 31 December 2017. The risk for cardiovascular events with hyperhidrosis and ETS was analyzed using Cox proportional hazards regression analysis. The risk for stroke was significantly higher in the hyperhidrosis group than in the control group (hazard ratio (HR), 1.28; 95% confidence interval (CI), 1.08-1.51); nonetheless, no significant difference in the risk for ischemic heart diseases was observed between the hyperhidrosis group and the control group (HR, 1.17; 95% CI, 0.99-1.31). Hyperhidrosis patients who did not undergo ETS were at significantly higher risk for cardiovascular events than the control group (HR, 1.28; 95% CI, 1.13-1.45). However, no significant difference in the risk for cardiovascular events was observed between hyperhidrosis patients who underwent ETS and the control group. Hyperhidrosis increases the risk for cardiovascular events. ETS could reduce this risk and needs to be considered for high-risk patients with cardiovascular diseases.


Assuntos
Doenças Cardiovasculares/epidemiologia , Endoscopia , Hiperidrose/epidemiologia , Hiperidrose/cirurgia , Simpatectomia , Procedimentos Cirúrgicos Torácicos , Adolescente , Adulto , Estudos de Coortes , Bases de Dados Factuais , Feminino , Humanos , Seguro Saúde , Masculino , República da Coreia/epidemiologia , Nervos Torácicos/cirurgia , Resultado do Tratamento , Adulto Jovem
7.
Biomaterials ; 224: 119496, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31557592

RESUMO

The decellularized tissue/organ extracellular matrix (dECM) is a naturally derived biomaterial that inherits various functional components from the native tissue or organ. Recently, various kinds of tissue/organ dECM bioinks capable of encapsulating cells, combined with 3D cell printing, have enabled remarkable progress in tissue engineering and regenerative medicine. However, the way in which the dECM component compositions of each tissue of different origins interact with cells and dictate tissue-specific cell behavior in the 3D microenvironment remains mostly unknown. To address this issue, in-depth differential proteomic analyses of four porcine dECMs were performed. Specifically, the differential variations of matrisome protein composition in each decellularized tissue type were also uncovered, which can play a significant role by affecting the resident cells in specific tissues. Furthermore, microarray analyses of human bone marrow mesenchymal stem cells (hBMMSCs) printed with various dECM bioinks were conducted to reveal the effect of compositional variations in a tissue-specific manner at the cellular level depending on the multipotency of MSCs. Through whole transcriptome analysis, differential expression patterns of genes were observed in a tissue-specific manner, and this research provides strong evidence of the tissue-specific functionalities of dECM bioinks.

8.
Adv Mater ; 31(41): e1904476, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31454108

RESUMO

Inserting a graft into vessels with different diameters frequently causes severe damage to the host vessels. Poor flow patency is an unresolved issue in grafts, particularly those with diameters less than 6 mm, because of vessel occlusion caused by disturbed blood flow following fast clotting. Herein, successful patency in the deployment of an ≈2 mm diameter graft into a porcine vessel is reported. A new library of property-tunable shape-memory polymers that prevent vessel damage by expanding the graft diameter circumferentially upon implantation is presented. The polymers undergo seven consecutive cycles of strain energy-preserved shape programming. Moreover, the new graft tube, which features a diffuser shape, minimizes disturbed flow formation and prevents thrombosis because its surface is coated with nitric-oxide-releasing peptides. Improved patency in a porcine vessel for 18 d is demonstrated while occlusive vascular remodeling occurs. These insights will help advance vascular graft design.


Assuntos
Oclusão de Enxerto Vascular/prevenção & controle , Fenômenos Mecânicos , Polímeros/farmacologia , Animais , Polímeros/química , Estresse Mecânico , Suínos
9.
J Clin Lipidol ; 13(4): 645-653.e2, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31126864

RESUMO

BACKGROUND: Previous studies indicate that circadian preference is associated with various energy metabolism and metabolic disorders. However, little is known about the associations between a circadian rhythm and blood lipid levels, especially in humans. OBJECTIVE: The aim of the study was to investigate whether the circadian rhythm affects serum lipid levels in Korean adults. METHODS: We designed a cross-sectional study to evaluate the associations between circadian preference and blood lipid levels in Korean adults. A total of 1984 participants (range of age 19-81 years) were included in this study. Propensity scores were calculated using logistic regression with age, sex, and body mass index. A total of 435 subjects were evaluated by propensity score matching analysis, equally distributed into morningness, intermediate, and eveningness groups, each with 145 subjects. Circadian preference was evaluated by the Morningness-Eveningness Questionnaire. RESULTS: Participants with the evening preference had significantly higher levels of total cholesterol, triglyceride, low-density lipoprotein cholesterol, and non-high-density lipoprotein cholesterol (non-HDL-C) when compared with those with morning or intermediate preference, after adjusting for confounding variables. Regarding other lipid parameters, both total cholesterol/HDL-C and low-density lipoprotein cholesterol/HDL-C in the evening preference are significantly higher than those of other circadian preferences. Evening preference was also significantly associated with a higher atherogenic index of plasma. CONCLUSION: Our study demonstrates that there is a significant association between circadian preference and blood lipid levels. Our findings suggest that individuals with evening preference could have a greater risk of atherosclerotic cardiovascular diseases.

10.
Breast J ; 25(4): 682-686, 2019 07.
Artigo em Inglês | MEDLINE | ID: mdl-31077484

RESUMO

The purpose of this study was to analyze the effectiveness of electron beam therapy (EBT) with patient-tailored bolus (PTB) using three-dimensional printing technology to reduce heart and lung doses during post-mastectomy radiotherapy (PMRT). For 28 patients with left breast cancer, we designed customized virtual bolus for PMRT to compensate for surface irregularities on computed tomography images and developed optimized plans for EBT. As comparison between the PTB and tangential plans, the PTB plan reduced unnecessary exposure to heart and ipsilateral lung with better target coverage compared with the tangential technique.


Assuntos
Coração , Radioterapia/métodos , Neoplasias Unilaterais da Mama/radioterapia , Neoplasias Unilaterais da Mama/cirurgia , Adulto , Idoso , Feminino , Humanos , Pulmão , Mastectomia , Pessoa de Meia-Idade , Tratamentos com Preservação do Órgão , Impressão Tridimensional , Radiodermatite , Radioterapia/efeitos adversos , Dosagem Radioterapêutica , Tomografia Computadorizada por Raios X , Neoplasias Unilaterais da Mama/diagnóstico por imagem
11.
Nanomaterials (Basel) ; 9(4)2019 Apr 10.
Artigo em Inglês | MEDLINE | ID: mdl-30974794

RESUMO

Recently, three-dimensional (3D) cell culture and tissue-on-a-chip application have attracted attention because of increasing demand from the industries and their potential to replace conventional two-dimensional culture and animal tests. As a result, numerous studies on 3D in-vitro cell culture and microfluidic chip have been conducted. In this study, a microfluidic chip embracing a nanofiber scaffold is presented. A electrospun nanofiber scaffold can provide 3D cell culture conditions to a microfluidic chip environment, and its perfusion method in the chip can allow real-time monitoring of cell status based on the conditioned culture medium. To justify the applicability of the developed chip to 3D cell culture and real-time monitoring, HepG2 cells were cultured in the chip for 14 days. Results demonstrated that the cells were successfully cultured with 3D culture-specific-morphology in the chip, and their albumin and alpha-fetoprotein production was monitored in real-time for 14 days.

12.
Neurotherapeutics ; 16(2): 523-537, 2019 04.
Artigo em Inglês | MEDLINE | ID: mdl-30680637

RESUMO

Ethyl pyruvate (EP) is a simple aliphatic ester of pyruvic acid and has been shown to have protective properties, which have been attributed to its anti-inflammatory, anti-oxidative, and anti-apoptotic functions. In an effort to develop better derivatives of EP, we previously synthesized DEOPA (N,N-diethyl-2-oxopropanamide, a novel isoster of EP) which has greater neuroprotective effects than EP, probably due to its anti-inflammatory and anti-excitotoxic effects. In the present study, we synthesized 3 DEOPA derivatives, in which its diethylamino group was substituted with diisopropylamino, dipropylamino, or diisobutylamino groups. Among them, DIPOPA (N,N-diisopropyl-2-oxopropanamide) containing diisopropylamino group had a greater neuroprotective effect than DEOPA or EP when administered intravenously to a rat middle cerebral artery occlusion (MCAO) model at 9 h after MCAO. Furthermore, DIPOPA had a wider therapeutic window than DEOPA and a marked reduction of infarct volume was accompanied by greater neurological and behavioral improvements. In particular, DIPOPA exerted robust anti-inflammatory effects, as evidenced by marked suppressions of microglia activation and neutrophil infiltration in the MCAO model, in microglial cells, and in neutrophil-endothelial cocultures at lower concentration, and did so more effectively than DEOPA. In particular, DIPOPA remarkably suppressed neutrophil infiltration into brain parenchyma, and this effect was attributed to the expressional inhibitions of cell adhesion molecules in neutrophils of brain parenchyma and in circulating neutrophils via NF-κB inhibition. Together, these results indicate the robust neuroprotective effects of DIPOPA are attributable to its anti-inflammatory effects and suggest that DIPOPA offers a potential therapeutic means of ameliorating cerebral ischemic injury and other inflammation-related pathologies.


Assuntos
Anti-Inflamatórios/uso terapêutico , Isquemia Encefálica/tratamento farmacológico , Infarto da Artéria Cerebral Média/tratamento farmacológico , Fármacos Neuroprotetores/uso terapêutico , Piruvatos/uso terapêutico , Animais , Anti-Inflamatórios/farmacologia , Masculino , Fármacos Neuroprotetores/farmacologia , Piruvatos/farmacologia , Ratos , Ratos Sprague-Dawley
13.
FASEB J ; 33(3): 4314-4326, 2019 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-30566396

RESUMO

The overactivity of cannabinoid 1 receptor (CB1R) is associated with obesity and type 2 diabetes. First-generation CB1R antagonists, such as rimonabant, offered therapeutic advantages for the control of obesity and related metabolic abnormalities, but their therapeutic potential was limited by undesirable neuropsychiatric side effects. Here, we evaluated AJ5012 as a novel potent peripheral CB1R antagonist and, using this antagonist, investigated the role of peripheral CB1R on adipose tissue inflammation in obese mouse models. AJ5012 had a high degree of CB1R and cannabinoid 2 receptor selectivity but a low brain:plasma concentration ratio without eliciting centrally mediated neurobehavioral effects. In diet-induced obese (DIO) mice, AJ5012 did not reduce food intake but did induce a significant weight loss, likely owing to an increased energy expenditure. It was as effective as rimonabant for the improvement of hormonal or metabolic abnormalities, glycemic control, and insulin sensitivity. The treatment of DIO and leptin receptor-deficient mice with AJ5012 also exhibited effects comparable to rimonabant for the prevention of macrophage infiltration, activation of the nucleotide-binding domain and leucine-rich repeat protein 3 inflammasome, and production of proinflammatory cytokines, which resulted in the suppression of adipose tissue inflammation. In addition to macrophage, activation of CB1R in 3T3-L1 adipocytes induced the expression of proinflammatory genes, which was fully inhibited by AJ5012. Our findings identified AJ5012 as a novel peripheral CB1R antagonist and suggest that peripheral CB1R blockade might break the links between insulin resistance and adipose tissue inflammation.-Han, J. H., Shin, H., Park, J.-Y., Rho, J. G., Son, D. H., Kim, K. W., Seong, J. K., Yoon, S.-H., Kim, W. A novel peripheral cannabinoid 1 receptor antagonist, AJ5012, improves metabolic outcomes and suppresses adipose tissue inflammation in obese mice.

14.
Adv Healthc Mater ; 7(23): e1801102, 2018 12.
Artigo em Inglês | MEDLINE | ID: mdl-30370670

RESUMO

3D printing technology is used to produce channels within hydrogels followed by endothelial cells (ECs)-seeding to establish in vitro vascular models. However, as built-in bulk hydrogels, it is difficult to incorporate additional cells and molecules into the crosslinked matrix to study the pathophysiological responses of healthy endothelium. In this study, freestanding in vitro vascular models (VMs) are developed using the coaxial cell printing technique and a vascular tissue-specific bioink. It has various advantages in plotting tubular cell-laden vessels with designed patterns, providing pump-driven circulating perfusion, generating endothelium without ECs-seeding, and implementing further expansions to study vascular pathophysiology. Following the maturation of endothelium, the VMs exhibit representative vascular functions (i.e., selective permeability, antiplatelets/leukocytes adhesion, and vessel remodeling under shear stress). Moreover, with the expansions of the VMs, the directional angiogenesis and inflammatory responses are demonstrated by giving asymmetric distributions of proangiogenic factors and an airway inflammatory ambience, respectively. Therefore, the freestanding, perfusable, and functional VMs can be useful devices to engineer diverse in vitro platforms for a wide range of biomedical applications, from modeling blood vessel relevant diseases to building vascularized tissues/organs.


Assuntos
Endotélio Vascular/química , Modelos Biológicos , Impressão Tridimensional , Alginatos/química , Adesão Celular , Citocinas/metabolismo , Endotélio Vascular/citologia , Endotélio Vascular/metabolismo , Células Endoteliais da Veia Umbilical Humana , Humanos , Poloxâmero/química , Engenharia Tecidual , Tecidos Suporte/química
15.
Biofabrication ; 11(1): 015002, 2018 10 30.
Artigo em Inglês | MEDLINE | ID: mdl-30270851

RESUMO

We used 3D cell printing to emulate an airway coupled with a naturally-derived blood vessel network in vitro. Decellularized extracellular matrix bioink derived from porcine tracheal mucosa (tmdECM) was used to encapsulate and print endothelial cells and fibroblasts within a designated polycarprolactone (PCL) frame. Providing a niche that emulates conditions in vivo, tmdECM gradually drives endothelial re-orientation, which leads to the formation of a lumen and blood vessel network. A fully-differentiated in vitro airway model was assembled with the printed vascular platform, and collectively reproduced a functional interface between the airway epithelium and the vascular network. The model presented respiratory symptoms including asthmatic airway inflammation and allergen-induced asthma exacerbation in physiological context. Because of the adaptable and automated nature of direct 3D cell printing, we expect that this will have relevance in vivo and high reproducibility for production of high-content platforms for preclinical trials in biomedical research.


Assuntos
Bioimpressão/métodos , Células Endoteliais/citologia , Fibroblastos/citologia , Impressão Tridimensional , Engenharia Tecidual/normas , Animais , Diferenciação Celular , Proliferação de Células , Matriz Extracelular/química , Matriz Extracelular/metabolismo , Humanos , Modelos Biológicos , Suínos , Traqueia/irrigação sanguínea , Traqueia/citologia
16.
Int J Epidemiol ; 47(6): 1821-1829, 2018 12 01.
Artigo em Inglês | MEDLINE | ID: mdl-30169829

RESUMO

Background: Osteoarthritis (OA) is the most common joint disease, but its association with mortality is unclear. Methods: We analysed data on adult participants in the 1988-94 and 1999-2010 National Health and Nutrition Examination Surveys, followed for mortality through 2011. OA was defined by self-report, and in a subset of participants 60 years or older with knee X-rays, radiographic knee OA (RKOA) was defined as Kellgren-Lawrence score ≥2. Cox proportional hazards were used to determine the mortality hazard ratio (HR) associated with self-reported OA and RKOA, adjusting for covariates. Results: The sample included 51 938 participants followed for a median 8.9 years; 2589 of them had knee X-rays and were followed for a median of 13.6 years. Self-reported OA and RKOA prevalences were 6.6% and 40.6%, respectively. Self-reported OA was not associated with mortality. RKOA was associated with an increased risk of mortality from cardiovascular diseases (CVD) {HR 1.43 [95% confidence interval (CI): 1.32, 1.64]}, diabetes [HR 2.04 (1.87, 2.23)] and renal diseases [HR 1.14 (1.04, 1.25)], but with a reduced risk of cancer mortality [HR 0.88 (0.80, 0.96)]. Participants with early RKOA onset (diagnosed before age 40) had a higher risk of mortality from all causes [HR 1.53 (1.43, 1.65)] and from diabetes [HR 7.18 (5.45, 9.45)]. Obese participants with RKOA were at increased risk of mortality from CVD [HR 1.89 (1.56, 2.29)] and from diabetes [HR: 3.42 (3.01, 3.88)]. Conclusions: Self-reported OA was not associated with mortality. RKOA was associated with higher CVD, diabetes and renal mortality, especially in people with early onset of the disease or with obesity.


Assuntos
Doenças Cardiovasculares/mortalidade , Diabetes Mellitus/mortalidade , Nefropatias/mortalidade , Obesidade/complicações , Osteoartrite do Joelho/complicações , Adulto , Idoso , Estudos de Coortes , Feminino , Humanos , Estimativa de Kaplan-Meier , Masculino , Pessoa de Meia-Idade , Neoplasias/mortalidade , Inquéritos Nutricionais , Prevalência , Modelos de Riscos Proporcionais , Fatores de Risco , Estados Unidos/epidemiologia
17.
Biomaterials ; 185: 276-283, 2018 12.
Artigo em Inglês | MEDLINE | ID: mdl-30261427

RESUMO

Extensive circumferential tracheal defects remain a major challenging problem in the field of tracheal reconstruction. In this study, a tissue-engineered tracheal graft based on three-dimensional (3D) printing was developed for extensive circumferential tracheal reconstruction. A native trachea-mimetic bellows scaffold, a framework for a tissue-engineered tracheal graft, was indirectly 3D printed and reinforced with ring-shaped bands made from medical grade silicone rubber. A tissue-engineered tracheal graft was then created by stratifying tracheal mucosa decellularized extracellular matrix (tmdECM) hydrogel on the luminal surface of the scaffold and transferring human inferior turbinate mesenchymal stromal cell (hTMSC) sheets onto the tmdECM hydrogel layer. The tissue-engineered tracheal graft with critical length was anastomosed end-to-end to the native trachea and complete re-epithelialization was achieved on the entire luminal surface within 2 months in a rabbit model with no post-operative complications. With this successful result, the present study reports the preliminary potential of the tissue-engineered tracheal graft as a rational tissue engineering strategy for extensive circumferential tracheal reconstruction.


Assuntos
Matriz Extracelular/química , Transplante de Células-Tronco Mesenquimais , Células-Tronco Mesenquimais/citologia , Engenharia Tecidual/métodos , Tecidos Suporte/química , Traqueia/citologia , Animais , Células Cultivadas , Humanos , Transplante de Células-Tronco Mesenquimais/métodos , Impressão Tridimensional , Coelhos , Procedimentos Cirúrgicos Reconstrutivos , Traqueia/química , Traqueia/cirurgia
18.
Korean J Anesthesiol ; 71(4): 311-316, 2018 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-29684991

RESUMO

BACKGROUND: Critical limb ischemia has been identified as a risk factor for the incidence of postoperative delirium in elderly patients. Limb amputation is the last option in critical limb ischemia treatments. We investigated the incidence and predisposing factors of postoperative delirium in patients undergoing major leg amputation. METHODS: From January 2012 to December 2016, 121 patients aged over 60 years who had undergone major leg amputation were enrolled in this study. Various factors related to the patients' outcomes were assessed, including demographic, preoperative laboratory, anesthetic, surgical, and postoperative indicators. RESULTS: Twenty two patients were excluded and 99 patients were assigned to either the delirium group or no delirium group. Forty of them (40%) developed a delirium during 30 days postoperatively. Univariate analysis implied that end-stage renal disease on hemodialysis, alcohol consumption, C-reactive protein, staying in an intensive care unit (ICU), duration of an ICU stay, occurrence of complications, and mortality during six months, were the factors that accounted for significant differences between the two groups. In multivariate analysis, three factors were significantly related to the development of delirium: mortality during six months (odds ratio [OR] = 13.86, 95% CI [2.10-31.90]), alcohol (OR = 8.18, 95% CI [1.13-16.60]), and hemodialysis (OR = 4.34, 95% CI [2.06-93.08]). CONCLUSIONS: Approximately 40% of the elderly patients suffered from postoperative delirium in major leg amputation. Identifying those with risk factors for postoperative delirium and intervening at the early stage will be of great benefit in major leg amputations for the elderly population.

19.
PLoS One ; 13(4): e0195784, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-29652920

RESUMO

To elucidate the association between renal hyperfiltration (RHF) and incident proteinuria, the data from 11,559,520 Korean adults who had undergone health screenings ≥ 3 times between 2009 and 2014 and had glomerular filtration rate (GFR) ≥60 mL/min/1.73m2 and negative dipstick test for proteinuria at baseline, were retrospectively analyzed. GFR was estimated with the Chronic Kidney Disease Epidemiology Collaboration equation based on serum creatinine, and RHF was defined as GFR >95th percentile adjusted for sex, age, body size, and diabetes and/or hypertension medication. The adjusted hazard ratio (aHR) of incident proteinuria in the RHF was 1.083 (95% CI, 1.069~1.097) compared to that of the non-RHF with Cox regression model. The association between RHF and incident proteinuria was not only in diabetic but also in non-diabetic subjects. This association was not observed in women (p for interaction <0.001). A reverse J-shaped association was found between the adjusted GFR slope and aHR of incident proteinuria. Both lower and higher GFR were associated with incident proteinuria in men. In conclusion, RHF was associated with incident proteinuria in men. Clinical studies are necessary to study whether the alleviation of RHF can prevent incident proteinuria.


Assuntos
Taxa de Filtração Glomerular , Rim/fisiopatologia , Proteinúria/etiologia , Adulto , Biomarcadores , Feminino , Humanos , Achados Incidentais , Masculino , Pessoa de Meia-Idade , Modelos de Riscos Proporcionais , Proteinúria/epidemiologia , Insuficiência Renal Crônica/etiologia , Insuficiência Renal Crônica/fisiopatologia , Fatores de Risco
20.
Biomaterials ; 156: 88-106, 2018 02.
Artigo em Inglês | MEDLINE | ID: mdl-29190501

RESUMO

Building human tissues via 3D cell printing technology has received particular attention due to its process flexibility and versatility. This technology enables the recapitulation of unique features of human tissues and the all-in-one manufacturing process through the design of smart and advanced biomaterials and proper polymerization techniques. For the optimal engineering of tissues, a higher-order assembly of physiological components, including cells, biomaterials, and biomolecules, should meet the critical requirements for tissue morphogenesis and vascularization. The convergence of 3D cell printing with a microfluidic approach has led to a significant leap in the vascularization of engineering tissues. In addition, recent cutting-edge technology in stem cells and genetic engineering can potentially be adapted to the 3D tissue fabrication technique, and it has great potential to shift the paradigm of disease modeling and the study of unknown disease mechanisms required for precision medicine. This review gives an overview of recent developments in 3D cell printing and bioinks and provides technical requirements for engineering human tissues. Finally, we propose suggestions on the development of next-generation therapeutics and diagnostics.


Assuntos
Materiais Biocompatíveis/química , Materiais Biocompatíveis/uso terapêutico , Impressão Tridimensional , Animais , Humanos , Tinta , Microfluídica , Neovascularização Fisiológica , Engenharia Tecidual
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