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1.
Braz J Biol ; 83: e244593, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34468512

RESUMO

': Kinnow' mandarin (Citrus nobilis L.× Citrus deliciosa T.) is an important marketable fruit of the world. It is mainstay of citrus industry in Pakistan, having great export potential. But out of total production of the country only 10% of the produce meets the international quality standard for export. Pre-harvest fruit drop and poor fruit quality could be associated with various issues including the plant nutrition. Most of the farmers do not pay attention to the supply of micro nutrients which are already deficient in the soil. Furthermore, their mobility within plants is also a question. Zinc (Zn) is amongst those micronutrients which affect the quality and postharvest life of the fruit and its deficiency in Pakistani soils is already reported by many researchers. Therefore, this study was carried out to evaluate the influence of pre-harvest applications of zinc sulfate (ZnSO4; 0, 0.4%, 0.6% or 0.8%) on pre-harvest fruit drop, yield and fruit quality of 'Kinnow' mandarin at harvest. The treatments were applied during the month of October i.e. 4 months prior to harvest. The applied Zn sprays had significant effect on yield and quality of the "Kinnow" fruit. Amongst different foliar applications of ZnSO4applied four months before harvest, 0.6% ZnSO4 significantly reduced pre-harvest fruit drop (10.08%) as compared to untreated control trees (46.45%). Similarly, the maximum number of fruits harvested per tree (627), fruit weight (192.9 g), juice percentage (42.2%), total soluble solids (9.5 °Brix), ascorbic acid content (35.5 mg 100 g-1) and sugar contents (17.4) were also found significantly higher with 0.6% ZnSO4 treatment as compared to rest of treatments and control. Foliar application of 0.6% ZnSO4 also significantly improved total antioxidants (TAO) and total phenolic contents (TPC) in fruit. In conclusion, foliar spray of ZnSO4 (0.6%) four months prior to harvest reduced pre-harvest fruit drop, increase yield with improved quality of 'Kinnow' mandarin fruit.


Assuntos
Citrus , Frutas , Antioxidantes , Ácido Ascórbico , Sulfato de Zinco/farmacologia
2.
Braz. j. biol ; 80(3): 565-573, July-Sept. 2020. tab
Artigo em Inglês | LILACS-Express | LILACS | ID: biblio-1132399

RESUMO

Abstract Due to increased number of herbicide resistant weeds, it is needed to explore the allelopathic potential of plants as an alternative. The research was conducted to investigate allelopathic effects of Carica papaya L. leaf powder and aqueous extract on seeds as well as pre-germinated seeds of Avena fatua L., Helianthus annuus L., Rumex dentatus L., Zea mays L. and Triticum aestivum L. on filter paper and soil in Weed Management Program Laboratory, Department of Plant and Environmental Protection at PARC Institute of Advanced Studies in Agriculture, National Agriculture Research Centre, Islamabad, Pakistan. Germination percentage (%), radicle length (cm) and plumule length (cm) were parameters observed for 'Plant leaf powder bioassay' and 'Aqueous extract method'. Most significant growth inhibition was observed in A. fatua seedlings in filter paper method. A. fatua radicle length was reduced by C. papaya aqueous extract (80%) and leaf powder (89%) bioassays. Plumule length was reduced under the influence of aqueous extract (57-73%) and powdered material (59-77%). The inhibitory effects on other test species were in sequence of H. annuus followed by Z. mays and R. dentatus. The aqueous extract showed non-significant effect on wheat seed germination, radicle and plumule growth. It is suggested that C. papaya aqueous extract can be used as source of weed management in wheat crop.


Resumo Devido ao aumento do número de ervas daninhas resistentes aos herbicidas, é necessário explorar o potencial alelopático das plantas como uma alternativa. A pesquisa foi conduzida com o objetivo de investigar os efeitos alelopáticos do pó foliar de Carica papaya e do extrato aquoso das sementes, bem como das sementes pré-germinadas de Avena fatua, Helianthus annuus, Rumex dentatus, Zea mays e Triticum aestivum em papel de filtro e solo no Laboratório do Programa de Manejo de Ervas Daninhas, Departamento de Plantas e Proteção Ambiental do Instituto PARC de Estudos Avançados em Agricultura, Centro Nacional de Pesquisa Agrícola, Islamabad, Paquistão. A porcentagem de germinação (%), o comprimento radicular e o comprimento da plúmula (cm) foram os parâmetros observados para o 'Bioensaio de Pó de Folha de Planta' e o 'Método de Extração Aquoso'. A maior inibição do crescimento foi observada em mudas de A. fatua no método de papel de filtro. O comprimento radicular de A. fatua foi reduzido com os extratos aquosos de C. papaya (80%) e pó de folhas (89%). O comprimento das plúmulas foi reduzido sob a influência do extrato aquoso (57-73%) e material em pó (59-77%). Os efeitos inibitórios em outras espécies-teste foram na sequência de H. annuus seguido por Z. mays e R. dentatus. O extrato aquoso apresentou efeito não significativo na germinação das sementes de trigo, nos crescimentos radiculares e das plúmulas. Sugere-se que o extrato aquoso de C. papaya pode ser utilizado como fonte de manejo de plantas daninhas na cultura do trigo.

3.
Braz J Biol ; 80(3): 565-573, 2020 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31644650

RESUMO

Due to increased number of herbicide resistant weeds, it is needed to explore the allelopathic potential of plants as an alternative. The research was conducted to investigate allelopathic effects of Carica papaya L. leaf powder and aqueous extract on seeds as well as pre-germinated seeds of Avena fatua L., Helianthus annuus L., Rumex dentatus L., Zea mays L. and Triticum aestivum L. on filter paper and soil in Weed Management Program Laboratory, Department of Plant and Environmental Protection at PARC Institute of Advanced Studies in Agriculture, National Agriculture Research Centre, Islamabad, Pakistan. Germination percentage (%), radicle length (cm) and plumule length (cm) were parameters observed for 'Plant leaf powder bioassay' and 'Aqueous extract method'. Most significant growth inhibition was observed in A. fatua seedlings in filter paper method. A. fatua radicle length was reduced by C. papaya aqueous extract (80%) and leaf powder (89%) bioassays. Plumule length was reduced under the influence of aqueous extract (57-73%) and powdered material (59-77%). The inhibitory effects on other test species were in sequence of H. annuus followed by Z. mays and R. dentatus. The aqueous extract showed non-significant effect on wheat seed germination, radicle and plumule growth. It is suggested that C. papaya aqueous extract can be used as source of weed management in wheat crop.


Assuntos
Carica , Germinação , Paquistão , Extratos Vegetais , Folhas de Planta , Sementes
4.
Epidemiol Infect ; 146(13): 1642-1653, 2018 10.
Artigo em Inglês | MEDLINE | ID: mdl-29986778

RESUMO

Co-circulation of Chikungunya and Dengue viral infections (CHIKV and DENV) have been reported mainly due to transmission by common Aedes vector. The purpose of the study was to identify and characterise the circulating strains of CHIKV and DENV in DENV endemic region of New Delhi during 2016. CHIKV and DENV were identified in the blood samples (n = 130) collected from suspected patients by RT-PCR. CHIKV was identified in 26 of 65 samples (40%). Similarly, DENV was detected in 48 of 120 samples (40%). Co-infection with both the viruses was identified in five (9%) of the samples. Interestingly, concurrent infection with DENV, CHIKV and Plasmodium vivax was detected in two samples. CHIKV strains (n = 11) belonged to the ECSA genotype whereas DENV-3 sequences (n = eight) clustered in Genotype III by phylogenetic analysis. Selection pressure of E1 protein of CHIKV and CprM protein of DENV-3 revealed purifying selection with four and two positive sites, respectively. Four amino acids of the CHIKV were positively selected and had high entropy suggesting probable variations. Co-circulation of both viruses in DENV endemic regions warrants effective monitoring of these emerging pathogens via comprehensive surveillance for implementation of effective control measures.


Assuntos
Febre de Chikungunya/epidemiologia , Vírus Chikungunya/isolamento & purificação , Coinfecção/epidemiologia , Vírus da Dengue/isolamento & purificação , Dengue/epidemiologia , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Febre de Chikungunya/virologia , Criança , Pré-Escolar , Coinfecção/virologia , Dengue/virologia , Feminino , Humanos , Índia/epidemiologia , Lactente , Recém-Nascido , Masculino , Pessoa de Meia-Idade , Filogenia , Adulto Jovem
5.
Soft Matter ; 13(10): 1988-1997, 2017 Mar 08.
Artigo em Inglês | MEDLINE | ID: mdl-28186520

RESUMO

Controlling the uptake of specific ions in polyelectrolyte multilayers is of interest for various fields of application. Here, we quantify the amount of cation of an ionic liquid, namely 1,3-bis(cyanomethyl)imidazolium chloride, incorporated into polyelectrolyte multilayers upon contact with an ionic liquid solution. The ion partition equilibrium is determined depending on concentration in solution, employing attenuated total reflection infrared spectroscopy. Generating an excess charge in multilayers by post-preparative manipulation of their charge balance, one can control the incorporated amount. Three multilayer systems are assembled for this purpose, i.e., PSS/PDADMAC, PSS/PAH and PAA/PDADMAC, employing poly(styrene sulfonate) (PSS), poly(diallyldimethylammonium chloride) (PDADMAC), poly(allylamine hydrochloride) (PAH) and poly(acrylic acid) (PAA). The charge balance of the latter two films is manipulated by an external pH stimulus generating an excess positive or negative internal charge, respectively. The concentration of cations in PEM amounts to 30% to 100% of the bulk concentration and scales as PAA/PDADMAC > PSS/PDADMAC > PSS/PAH. Thus, post-preparative pH treatment may be a future tool to create ion-conductive polymer gel films with a desired concentration of small cations.

8.
Saf Health Work ; 4(1): 27-36, 2013 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-23516114

RESUMO

OBJECTIVES: Mental health has been shown to be linked with certain underlying physiological mechanisms. The objective of this cross sectional study was to investigate the relationship between depressive symptoms and brachial artery reactivity (BAR) in an understudied population: police officers. METHODS: Participants were 351 police officers who were clinically examined in the Buffalo Cardio-Metabolic Police Stress (BCOPS) study. BAR was performed using standard B-Mode ultrasound procedures. Depressive symptoms were measured using the Center for Epidemiological Studies Depression (CES-D) scale. Mean values of the difference between the baseline and maximum diameters of the brachial artery were determined across three categories of CES-D score using the analysis of variance and the analysis of covariance. p-values for linear trends were obtained from linear regression models. RESULTS: The mean age (± standard deviation) of all officers was 40.9 ± 7.2 years. Women had a slightly higher mean CES-D score than men (8.9 ± 8.9 vs. 7.4 ± 6.4) and a slightly higher percentage increase of BAR than men (6.90 vs. 5.26%). Smoking status significantly modified the associations between depressive symptoms and BAR. Among current smokers, mean absolute values of BAR significantly decreased as depressive symptoms increased after adjustment for age, gender, race/ethnicity, hypertension, and diabetes; the multivariate-adjusted p-values were 0.033 (absolute) and 0.040 (%). Associations between depressive symptoms and BAR were not statistically significant among former smokers or never smokers. CONCLUSION: Depressive symptoms were inversely associated with BAR among police officers who were current smokers and together may be considered a risk factor for cardiovascular disease among police officers. Further prospective research is warranted.

9.
Neurochem Res ; 37(1): 202-4, 2012 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-21935729

RESUMO

The activities of Glutamate decarboxylase (GAD) and Gamma aminobutyric acid (GABA) were studied in three regions of rat brain in heightened neuronal activity resulting in convulsions by Leptazol. These enzymes were studied in preconvulsive, convulsive and post convulsive phases. The activity of GAD decreases significantly in the preconvulsive phase in all the three regions of brain followed by a significant increase during the convulsive and post convulsive phase in cerebral cortex and cerebellum. The activity of GABA-T decreases maximal during the preconvulsive phase followed by convulsive phase. The activity of this enzyme tended to increase to control values when the postconvulsive phase was reached. Therefore, it is suggested that the concomitant decrease of GAD activity and GABA concentration, is probably an important factor in the onset of convulsions.


Assuntos
4-Aminobutirato Transaminase/metabolismo , Encéfalo/enzimologia , Glutamato Descarboxilase/metabolismo , Pentilenotetrazol/efeitos adversos , Convulsões/enzimologia , Animais , Masculino , Ratos , Convulsões/induzido quimicamente
10.
J Xray Sci Technol ; 19(4): 423-8, 2011.
Artigo em Inglês | MEDLINE | ID: mdl-25214377

RESUMO

Pneumonia is the common type of infection found in the world. The infection spreads in the lungs area of a human body. The chest x-ray is performed to diagnose this infection. Physicians use this X-ray image to diagnose or monitor treatment for conditions of pneumonia. This type of chest X-ray is also used in the diagnosis of diseases like emphysema, lung cancer, line and tube placement and tuberculosis. Feature extraction methods like DWT, WFT, and WPT can also be used. In this paper, detection of pneumonia infection by unsupervised fuzzy c-means classification learning algorithm is used. This approach gives better result than the rest of the methods. In fuzzy c-means, each resultant pixel gives accurate value since it has a weight associated with it.


Assuntos
Lógica Fuzzy , Pneumonia/diagnóstico por imagem , Interpretação de Imagem Radiográfica Assistida por Computador/métodos , Radiografia Torácica/métodos , Análise por Conglomerados , Humanos
11.
Curr Pharm Biotechnol ; 12(2): 226-30, 2011 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-21044003

RESUMO

Liver failure results in impairment of many functions and dependent organs such as brain and kidneys begin to fail, reducing the chance of recovery even further. Orthotopic liver transplantation (OLTx) is the only treatment that improves the survival rate in patients with liver failure. Liver Transplantation (LT), including orthologous liver transplantation (OLT), cadaveric LT, split LT, living donor LT (LDLT) brings hopes to patients with these diseases. Globally, 1.4 million deaths occur annually as a result of chronic liver diseases. The reasons for this high death toll include unavailability of healthy liver donor and highly expensive liver transplantation treatment. Furthermore, some other factors such as operative risks and post-transplant rejection are major limitation of OLT. Isolated hepatocyte transplantation is emerging as alternative bridge support till the healthy donor is arranged. Mature hepatocytes have several drawbacks such as low proliferation both in vitro and in vivo, low viability after cryopreservation, and requirement of large number of cells for infusion. The studies on isolation of hepatic progenitors have shown promising results to overcome these limitations. These cells possess higher proliferative capacity, are less immunogenic and more resistant to cryopreservation, and ischemic injury; properties that could enhance their engraftment within the recipient liver. The hepatic progenitors have been isolated from the intra-hepatic sources and extra-hepatic sources. Fetal cells are one of the ideal sources of hepatic stem/progenitor cells. Autologous bone marrow stem cell transplantation in patients with cirrhosis has shown promising result.


Assuntos
Hepatócitos/citologia , Cirrose Hepática/terapia , Hepatopatias/terapia , Falência Hepática/terapia , Transplante de Fígado , Erros Inatos do Metabolismo/terapia , Células-Tronco/fisiologia , Rejeição de Enxerto , Hepatócitos/transplante , Humanos , Cirrose Hepática/cirurgia , Hepatopatias/cirurgia , Falência Hepática/cirurgia , Células-Tronco Mesenquimais/citologia , Células-Tronco Mesenquimais/fisiologia , Transplante de Células-Tronco , Células-Tronco/citologia , Fatores de Tempo
12.
Cell Transplant ; 19(4): 409-18, 2010.
Artigo em Inglês | MEDLINE | ID: mdl-20447340

RESUMO

Liver transplantation is the only existing modality for treating decompensated liver cirrhosis. Several factors, such as nonavailability of donors, combined with operative risks, complications associated with rejection, usage of immunosuppressive agents, and cost intensiveness, make this strategy available to only a few people. With a tremendous upsurge in the mortality rate of patients with liver disorders worldwide, there is a need to search for an alternative therapeutic tool that can combat the above limitations and serve as a supportive therapy in the management of liver diseases. Cell therapy using human fetal liver-derived stem cells can provide great potential to conservatively manage end-stage liver diseases. Therefore, the present investigation aimed to study and prove the safety and efficacy of human fetal liver-derived stem cell transplantation in patients with end-stage liver cirrhosis. Twenty-five patients with liver cirrhosis of different etiologies were infused with human fetal liver-derived stem cells (EpCAM+ve) labeled with Tc-HMPAO through hepatic artery. Our high throughput analysis using flow cytometry, RT-PCR, and cellular characterization exemplifies fetal liver cells with their high proliferation rate could be the best source for rejuvenating the diseased liver. Further, no episodes related to hepatic encephalopathy recurred in any of the subjects following hepatic stem cell transplantation. There was marked clinical improvement observed in terms of all clinical and biochemical parameters. Further, there was decrease in mean MELD score (p < 0.01) observed in 6 months follow-up in all patients. Therapy using human fetal liver stem/progenitor cells offers a potentially supportive modality to organ transplantation in the management of liver diseases.


Assuntos
Células-Tronco Fetais/transplante , Cirrose Hepática/terapia , Fígado/citologia , Adulto , Biomarcadores/metabolismo , Terapia Baseada em Transplante de Células e Tecidos , Células-Tronco Fetais/citologia , Citometria de Fluxo , Humanos , Masculino , Pessoa de Meia-Idade , Índice de Gravidade de Doença , Transplante de Células-Tronco , Tecnécio Tc 99m Exametazima
13.
Diabetes Technol Ther ; 12(5): 373-8, 2010 May.
Artigo em Inglês | MEDLINE | ID: mdl-20388047

RESUMO

BACKGROUND: beta-Cell destruction and/or insufficient insulin production are the hallmarks of diabetes mellitus (type 1 diabetes). A hepatic progenitor from developing liver is sought to be one of the surrogate sources of insulin production as the pancreas and the liver share a common precursor and signals from the cardiac mesoderm. Production of insulin is possible by transfecting pancreatic transcription factors that play important roles in development of the pancreatic beta-cell. But, there is always the fear of using genetically manipulated cells for therapeutics. Hence, the present study was designed to analyze the feasibility of using primary human fetal hepatic progenitors as a potential source for insulin production. METHODS: Human fetal hepatic progenitors were enriched using CD-326 magnetic cell sorting. The sorted cells were cultured with different concentrations of glucose (5-30 mM) in Dulbecco's modified Eagle's medium. The amount of insulin production was estimated in the cultured cells by the chemiluminescence method. Total RNA isolated from sorted epithelial cell adhesion molecule (EpCAM)-positive cells was reverse-transcribed, and the expression of different beta-cell-producing transcriptions factors was analyzed by polymerase chain reaction (PCR). Immunocytochemical analysis was performed in cultured cells using specific insulin antibodies. RESULTS: The viability of the total liver cells isolated was found to be 95%. The average number of EpCAM-positive cells in the total liver was found to be approximately 15%. An insulin kinetics study using glucose induction with different concentrations showed increased insulin secretion in response to glucose concentrations up to 20 mM. Furthermore, results of immunocytochemical analysis demonstrated intense insulin expression in EpCAM-positive cultured cells. Expression studies of the cultured EpCAM-positive cells using reverse transcription-PCR showed positive expression of the pancreatic transcription factors essential for insulin production. CONCLUSIONS: The present study demonstrates that in vitro differentiation of induced human hepatic progenitors into insulin-producing cells without genetic manipulations may promote strategies for the treatment of type 1 diabetes.


Assuntos
Hepatócitos/metabolismo , Insulina/metabolismo , Pâncreas/metabolismo , Células Cultivadas , Expressão Gênica , Hepatócitos/citologia , Humanos , Imuno-Histoquímica , Secreção de Insulina , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Fatores de Transcrição/genética , Fatores de Transcrição/metabolismo
14.
Psychophysiology ; 46(2): 293-9, 2009 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-19207200

RESUMO

The relationship between cardiovascular stress reactivity and carotid artery intima-media thickness (IMT) has been established in adults, but not yet studied in children. Cardiovascular reactivity to an ad lib speech was measured in 20 boys and 20 girls age 11.0 +/- 1.4 years. Measures included heart rate (HR), systolic (SBP) and diastolic (DBP) blood pressure reactivity, and mean common carotid artery IMT. Sequential regression analyses were used to establish the incremental increase in R(2) for the prediction of IMT due to cardiovascular reactivity independent of age, socioeconomic status, race, percentage body fat, and baseline BP or HR. SBP reactivity (beta=0.002, , p<.05), but not DBP reactivity (p=.12) or HR reactivity (p=.82), independently predicted carotid artery IMT. This study provides initial evidence that SBP reactivity is associated with IMT and perhaps the early pathogenesis of cardiovascular disease in childhood.


Assuntos
Artérias Carótidas/patologia , Hemodinâmica/fisiologia , Estresse Psicológico/fisiopatologia , Antropometria , Pressão Sanguínea/fisiologia , Composição Corporal/fisiologia , Artérias Carótidas/diagnóstico por imagem , Criança , Feminino , Humanos , Masculino , Meio Social , Ultrassonografia
15.
Transplant Proc ; 40(4): 1140-4, 2008 May.
Artigo em Inglês | MEDLINE | ID: mdl-18555134

RESUMO

This study was performed to determine the safety and tolerability of injecting autologous bone marrow stem cells (BMC) (CD34+) into four patients with liver insufficiency. The study was based on the hypothesis that the CD34+ cell population in granulocyte colony stimulating factor (G-CSF) mobilized blood and autologous bone marrow contains a subpopulation of cells with the potential for regenerating damaged tissue. We separated the CD34+ stem cell population from the bone marrow. The potential of the BMC to differentiate into hepatocytes and other cell lineages has already been reported. Several reports have also demonstrated the plasticity of hematopoietic stem cells to differentiate into hepatocytes. Recently Sakaida demonstrated reduction in fibrosis in chemically induced liver cirrhosis following BMC transplantation. From a therapeutic point of view, chronic liver cirrhosis is one of the targets for BMC transplantation. In this condition, there is excessive deposition of extracellular matrix and hepatocyte necrosis. Encouraged by this evidence that the CD34+ cell population contains cells with the potential to form hepatocyte-like elements, four patients with liver insufficiency were given G-CSF to mobilize stem cells. CD34+ cells (0.1 x 10(8)) were injected into the hepatic artery. No complications or specific side effects related to the procedure were observed; four patients showed improvements in serum albumin, bilirubin and ALT after one month from the cell infusion.


Assuntos
Transplante de Medula Óssea , Falência Hepática/cirurgia , Segurança , Transplante de Células-Tronco , Adulto , Diferenciação Celular , Doença Crônica , Feminino , Citometria de Fluxo , Fator Estimulador de Colônias de Granulócitos/uso terapêutico , Mobilização de Células-Tronco Hematopoéticas/métodos , Artéria Hepática , Hepatócitos/citologia , Humanos , Infusões Intravenosas , Masculino , Pessoa de Meia-Idade , Seleção de Pacientes , Transplante Autólogo , Resultado do Tratamento
16.
Transplant Proc ; 40(4): 1148-50, 2008 May.
Artigo em Inglês | MEDLINE | ID: mdl-18555136

RESUMO

Crigler-Najjar Syndrome (CNS) is characterized by mild, chronic unconjugated hyperbilirubinemia resulting from an autosomal-recessive inherited deficiency of hepatic uridine/diphosphoglucuronate-glucuronosyl transferase 1Al since birth. Herein we have reported a confirmed case of CNS type 1 in a 2-year-old girl with an unconjugated hyperbilirubinemia (>30 mg/dL) treated by hepatic progenitor cell infusion through the hepatic artery. No procedure-related complications were encountered. No kernicterus was observed. The total bilirubin started falling at 10 days after cell infusion. Two months after cell infusion the bilirubin fell from 29.0 to 16 mg/dL, with the conjugated bilirubin increasing approximately fivefold, the unconjugated bilirubin decreasing nearly twofold, and the SGPT also decreasing from 210 U/L to 64 U/L. This study demonstrated the efficacy of hepatic progenitor cells to manage hyperbilirubinemia in these patients. As the procedure is simple and the patient has tolerated the cell therapy, infusion can be repeated as required to manage hyperbilirubinemia, which often causes lethal kernicterus. This study was developed to assess the safety, feasibility, and efficacy of hepatic progenitor cell transplantation in a child with CNS type 1.


Assuntos
Síndrome de Crigler-Najjar/cirurgia , Hepatócitos/transplante , Hiperbilirrubinemia/cirurgia , Transplante de Células-Tronco/métodos , Animais , Bilirrubina/sangue , Pré-Escolar , Síndrome de Crigler-Najjar/sangue , Síndrome de Crigler-Najjar/genética , Modelos Animais de Doenças , Feminino , Transplante de Tecido Fetal , Glucuronosiltransferase/genética , Artéria Hepática , Humanos , Hiperbilirrubinemia/sangue , Hiperbilirrubinemia/genética , Reação em Cadeia da Polimerase
17.
Transplant Proc ; 40(4): 1153-5, 2008 May.
Artigo em Inglês | MEDLINE | ID: mdl-18555137

RESUMO

Cholangiodestruction of bile ducts leads to biliary atresia, a rare disease characterized by intrahepatic and extrahepatic biliary inflammation. If the intrahepatic biliary tree is unaffected, surgical reconstruction by the Kasai procedure of hepatoportoenterostomy of the extra hepatic biliary tract is possible. Untreated, this condition leads to cirrhosis and death within the first year of the life. If the atresia is complete, liver transplantation is the only option. As a result of the shortage of donor livers, hepatocytes have been infused over the past two decades, providing proof of the concept that cell therapy can be effective for the treatment of liver diseases. In the present study, we report a confirmed case of a girl of 1 year of age with increased bilirubin of 28.5 mg/dL and pediatric end-stage liver disease score 20. Biochemical liver function tests showed cholestasis (elevated cholesterol and gamma-GTs) and increased ALT, total bilirubin, conjugated bilirubin, and ALP. The patient was treated with hepatic progenitor cell infusion through the hepatic artery. The total bilirubin and conjugated bilirubin started decreasing during the first month after cell infusion. The level of total bilirubin maintained a threefold decrease after months of cell infusion. The conjugated bilirubin was 16.35 mg/dL before cell infusion, decreasing to eightfold after cell infusion. After 2 months of cell infusion, hepatobiliary scintigraphy showed increased liver cell function. This case demonstrated the efficacy and functionality of hepatic progenitor cells for the management of biliary atresia. Further, as there was a decrease in serum bilirubin, it showed that there was some percentage of the engraftment of the infused cells. As the procedure is simple and the patient has tolerated the infusion therapy, it might be repeated to manage biliary atresia.


Assuntos
Transplante de Tecido Fetal/métodos , Artéria Hepática , Hepatócitos/transplante , Hiperbilirrubinemia/cirurgia , Transplante de Células-Tronco/métodos , Alanina Transaminase/sangue , Bilirrubina/sangue , Feminino , Humanos , Hiperbilirrubinemia/sangue , Hiperbilirrubinemia/enzimologia , Lactente , Fígado/enzimologia , Testes de Função Hepática
19.
Transplant Proc ; 39(3): 694-9, 2007 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-17445575

RESUMO

Stem cell research is a new field that is advancing at an incredible pace with new discoveries being reported from all over the world. Scientists have for years looked for ways to use stem cells to replace cells and tissues that are damaged or diseased. Stem cells are the foundation cells for every organ, tissue, and cell in the body. Stem cells are undifferentiated, "blank" cells that do not yet have a specific function. Under proper conditions, stem cells begin to develop into specialized tissues and organs. They are self-sustaining and can replicate themselves for long periods of time. Embryonic stem cells are pluripotent cells, isolated from the inner cell mass of the blastocyst-stage mammalian embryo. They have the ability to differentiate into several somatic or somatic-like functional cells such as neurons, hepatocytes, cardiomyocytes, and others. Adult stem cells are specialized cells found within many tissues of the body where they function in tissue homeostasis and repair. They are precursor cells capable of differentiation into several different cells. The knowledge of stem cells from various sources offered a new hope for the treatment of various diseases.


Assuntos
Transplante de Células-Tronco/tendências , Adulto , Animais , Diabetes Mellitus/terapia , Células-Tronco Embrionárias/citologia , Cardiopatias/terapia , Humanos , Fígado/citologia , Mesoderma/citologia , Modelos Animais , Modelos Biológicos , Doenças do Sistema Nervoso/terapia , Transplante de Células-Tronco/etnologia
20.
Indian J Med Res ; 123(5): 601-14, 2006 May.
Artigo em Inglês | MEDLINE | ID: mdl-16873904

RESUMO

Acute liver failure (ALF) carries high morbidity and mortality (>80%) even in the best centres. Orthotopic liver transplantation (OLTx) is the only viable approach to the treatment of ALF. This has significantly improved the survival in these patients. The major limitations of OLTx are non availability of the donor liver, requirement of a major surgical procedure, high cost and longterm immunosuppression. Isolated hepatocyte transplantation is emerging as an appealing method for the treatment of ALF because of its technical simplicity and easy availability of cells. Transplantation of allogenic/xenogenic hepatocytes transplantation in experimentally induced ALF has shown an increased survival rate. Clinical studies in acute, chronic liver failure and metabolic disorders have also been undertaken in a few centres and have shown encouraging results. To maintain the continuous supply of cells, xenogenic source of hepatocytes (porcine, rabbit, canine) have offered a hope. A major concern regarding the use of xenogenic donors is the risk of transmission of zoonosis and immunogenicity. Recently, Porcine endogenous retrovirus (PERV) has been shown to infect human tissue in vitro. The problem of immunogenicity of xenogenic hepatocytes can be overcome to some extent by immunoisolation, encapsulation technique, which may also provide protection to the hepatocytes during cryopreservation. The knowledge of adult hepatic stem from tissue offered a new hope for the treatment of various chronic and metabolic diseases. Further, the transdifferentiation potentiality of haematopoietic stem cells to hepatic lineage has strengthened cell therapy.


Assuntos
Hepatócitos/transplante , Hepatopatias/terapia , Transplante de Células-Tronco , Animais , Anticorpos Heterófilos , Órgãos Artificiais , Separação Celular , Hepatócitos/imunologia , Hepatócitos/efeitos da radiação , Humanos , Falência Hepática/terapia , Transplante de Fígado , Camundongos , Ratos , Transplante Heterólogo , Transplante Homólogo , Trissacarídeos , Raios Ultravioleta
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