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1.
Biomed Mater ; 2020 Feb 13.
Artigo em Inglês | MEDLINE | ID: mdl-32053805

RESUMO

We developed polymeric scaffolds that can provide both topographical and electrical stimuli on mouse neural stem cells (mNSCs) for potential use in nerve tissue engineering. In contrast to conventional patterning techniques such as imprinting, soft/photolithography, and three-dimensional printing, microgroove patterns were generated by using aligned electrospun fibers as templates, via a process denoted as electrospun fiber-template lithography (EFTL). The preparation of polyvinylpyrrolidone (PVP) fibers, followed by the deposition of poly(lactic-co-glycolic acid) (PLGA) and the removal of the fiber template, produced freestanding PLGA scaffolds with microgrooves having widths of 1.72 ± 0.24 µm. The subsequent deposition of polypyrrole (PPy) via chemical oxidative polymerization added conductivity to the microgrooved PLGA scaffolds. The resultant scaffolds were cytocompatible with mNSCs. The microgroove patterns enhanced the alignment and elongation of mNSCs, and the PPy layer promoted the interaction of cells with the surface by forming more and longer filopodia compared with the nonconductive surface. Finally, the neuron differentiation of mNSCs was evaluated by monitoring the Tuj-1 neuronal gene expression. The presence of both microgrooves and the conductive PPy layer enhanced the neuronal differentiation of mNSCs even without electrical stimulation, and the neuronal differentiation was further enhanced by stimulation with a sufficient electrical pulse (1.0 V).

2.
Biomacromolecules ; 21(1): 143-151, 2020 Jan 13.
Artigo em Inglês | MEDLINE | ID: mdl-31559819

RESUMO

A poly(ethylene glycol)-based thermogel can capture an iron ion (Fe3+) through a crown ether-like coordination bond between the oxygen atom and metal ions, thus, providing a sustained Fe3+-releasing system. Poly(ethylene glycol)-l-poly(alanine) thermogel was used in this study. The polypeptide forms a rather robust gel, and the degradation products are a neutral amino acid, which provides cyto-compatible neutral pH environments during the cell culture. During the heat-induced sol-to-gel transition at 37 °C, tonsil-derived mesenchymal stem cells (TMSCs) and iron ions were incorporated, leading to the formation of a three-dimensional matrix toward neuronal differentiation of the incorporated TMSCs. The initial concentration of the iron ions was varied between 0, 15, 30, and 60 mM. About 10% of the loaded iron ions was released over 21 days, which continuously supplied iron ions to the cells. The incorporation of iron ions not only increased the gel modulus at 37 °C from 107 to 680 Pa, but also promoted cell aggregation with a significant secretion of the cell adhesion signal of FAK. Expression of biomarkers related to the neuronal differentiation of TMSCs, including NFM, MAP2, GFAP, NURR1, NSE, and TUBB3, increased 4-35-fold at the mRNA level in the Fe3+-containing system compared to that of the system without Fe3+. Immunofluorescence studies also confirmed pronounced cell aggregation and a significant increase in neuronal biomarkers at the protein level. This study suggests that an iron ion-releasing thermogelling system can be a promising injectable scaffold toward neuronal differentiation of stem cells.

3.
Biomaterials ; 159: 91-107, 2018 03.
Artigo em Inglês | MEDLINE | ID: mdl-29316455

RESUMO

Thermogel is an aqueous polymer solution that undergoes sol-to-gel transition as the temperature increases. Cells, growth factors, and signaling molecules can be incorporated simultaneously during the sol-to-gel transition. The cytocompatible procedure makes the thermogel an excellent platform for 3D culture of stem cells. This review focuses on the crucial questions that need to be addressed to achieve effective differentiation of stem cells into target cells, comprising low modulus, cell adhesion, and controlled supply of the growth factors. Recent progress in the use of thermogel as a 3D culture system of stem cells is summarized, and our perspectives on designing a new thermogel for 3D culture and its eventual application to injectable tissue engineering of stem cells are presented.


Assuntos
Polímeros/química , Células-Tronco/citologia , Engenharia Tecidual/métodos , Adesão Celular/fisiologia , Diferenciação Celular/fisiologia , Temperatura Ambiente
4.
Tissue Eng Regen Med ; 15(5): 521-530, 2018 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-30603576

RESUMO

Background: Thermogel is an aqueous solution that exhibits a sol-to-gel transition as the temperature increases. Stem cells, growth factors, and differentiating factors can be incorporated in situ in the matrix during the sol-to-gel transition, leading to the formation of a three-dimensional (3D) cell-culture scaffold. Methods: The uses of thermogelling polypeptides, such as collagen, Matrigel™, elastin-like polypeptides, and synthetic polypeptides, as 3D scaffolds of cells, are summarized in this paper. Results: The timely supply of growth factors to the cells, cell survival, and metabolite removal is to be insured in the cell culture matrix. Various growth factors were incorporated in the matrix during the sol-to-gel transition of the thermogelling polypeptide aqueous solutions, and preferential differentiation of the incorporated stem cells into specific target cells were investigated. In addition, modulus of the matrix was controlled by post-crosslinking reactions of thermogels or employing composite systems. Chemical functional groups as well as biological factors were selected appropriately for targeted differentiation of the incorporated stem cells. Conclusion: In addition to all the advantages of thermogels including mild conditions for cell-incorporation and controlled supplies of the growth factors, polypeptide thermogels provide neutral pH environments to the cells during the degradation of the gel. Polypeptide thermogels as an injectable scaffold can be a promising system for their eventual in vivo applications in stem cell therapy.

5.
Polymers (Basel) ; 10(10)2018 Sep 28.
Artigo em Inglês | MEDLINE | ID: mdl-30961003

RESUMO

In the field of tissue engineering, conductive hydrogels have been the most effective biomaterials to mimic the biological and electrical properties of tissues in the human body. The main advantages of conductive hydrogels include not only their physical properties but also their adequate electrical properties, which provide electrical signals to cells efficiently. However, when introducing a conductive material into a non-conductive hydrogel, a conflicting relationship between the electrical and mechanical properties may develop. This review examines the strengths and weaknesses of the generation of conductive hydrogels using various conductive materials such as metal nanoparticles, carbons, and conductive polymers. The fabrication method of blending, coating, and in situ polymerization is also added. Furthermore, the applications of conductive hydrogel in cardiac tissue engineering, nerve tissue engineering, and bone tissue engineering and skin regeneration are discussed in detail.

6.
J Clin Diagn Res ; 11(8): QD08-QD09, 2017 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-28969217

RESUMO

Various types of neurogenic tumours can involve the abdomen. Retroperitonial space is most commonly affected but other sites can also be involved. Mostly younger age group is affected and malignant transformation is rare. CT and MRI are helpful in making diagnosis and planning surgery. Complete surgical excision is treatment of choice. Final diagnosis is made by histopathology and immunohistochemistry report. We report a rare case of giant solitary benign neurofibroma of anterior abdominal wall in 25-year-old multiparous women reported on day 15 of vaginal delivery with large abdominal mass which was masked before because of pregnancy.

7.
ACS Chem Neurosci ; 8(7): 1455-1458, 2017 07 19.
Artigo em Inglês | MEDLINE | ID: mdl-28452458

RESUMO

Nuclear receptor related 1 (NURR1) is an essential protein for maintenance of dopaminergic neurons in adult midbrain of which deficiency leads to Parkinson's disease. To enhance the NURR1 production of neural cells, various approaches are under investigation. Here we report that NURR1 is highly expressed in stem cells by exposure to an L-polarized blue light emitting diode (LED). Compared to stem cells cultured in the absence of a LED, under polarized green and red LEDs, the stem cells exposed to a polarized blue LED significantly enhanced neuronal biomarkers such as neurofilament M (NFM) and neuron specific enolase (NSE) at both mRNA and protein levels. In particular, NURR1 was selectively enhanced by the stem cells exposed to the L-polarized blue LED. Stem cells exposed to the L-polarized blue LED increased mitochondrial ATP and intracellular calcium ions, which support neuronal differentiation of the stem cells. This study suggests that chiro-optical treatments by using polarized light with a specific wavelength can be used for engineering of stem cells with enhanced specific biochemicals, which may open a new method for a specific disease.


Assuntos
Luz , Células-Tronco Mesenquimais/metabolismo , Neurogênese , Membro 2 do Grupo A da Subfamília 4 de Receptores Nucleares/biossíntese , Trifosfato de Adenosina/metabolismo , Cálcio/metabolismo , Técnicas de Cultura de Células/instrumentação , Sobrevivência Celular , Criança , Feminino , Imunofluorescência , Expressão Gênica/efeitos da radiação , Humanos , Células-Tronco Mesenquimais/citologia , Células-Tronco Mesenquimais/efeitos da radiação , Mitocôndrias/metabolismo , Mitocôndrias/efeitos da radiação , Proteínas de Neurofilamentos/biossíntese , Proteínas de Neurofilamentos/genética , Membro 2 do Grupo A da Subfamília 4 de Receptores Nucleares/genética , Tonsila Palatina , Fosfopiruvato Hidratase/biossíntese , Fosfopiruvato Hidratase/genética , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , Reação em Cadeia da Polimerase em Tempo Real
8.
J Clin Diagn Res ; 10(8): QC18-21, 2016 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-27656511

RESUMO

INTRODUCTION: The intensity of pain experienced by women in labour, has been found to affect the progress of labour, foetal well-being and maternal psychology. Adverse effects associated with commonly used opioids for providing intrapartum analgesia have created a need for an alternative non-opioid drug. AIM: To evaluate the efficacy of an intravenous infusion of 1000 mg of acetaminophen as an intrapartum analgesic. MATERIALS AND METHODS: The present prospective single-centre, single blind, placebo-controlled randomized interventional study was conducted in Department of Obstetrics and Gynaecology in Vardhaman Mahavir Medical College & Safdarjung Hospital over a period of six months from September 2014 to March 2015. After receiving the ethical clearance and written informed consent. The first 200 consecutive parturients fulfilling the inclusion criteria were recruited into the study. Women were then randomised to receive either intravenous 1000 mg (100ml) of acetaminophen (Group A, n=100) or 100 ml normal saline (Group B, n=100). Primary outcome assessed was effectiveness of acetaminophen to provide an adequate amount of analgesia, as measured by a change in Visual Analogue Scale (VAS) pain intensity score at various times after drug administration. Secondary outcomes measured were duration of labour, need for additional rescue analgesia and presence of adverse maternal or foetal effect. RESULTS: There was pain reduction at 1 and 2 hours in both groups (p<0.001). However, it was more significant in the acetaminophen group, especially at 1 hour. Duration of labour was shortened in both the groups, without any maternal and foetal adverse effects. CONCLUSION: Intravenous acetaminophen is an efficacious non-opioid drug for relieving labour pain without any significant maternal and foetal adverse effects.

9.
ACS Appl Mater Interfaces ; 8(8): 5160-9, 2016 Mar 02.
Artigo em Inglês | MEDLINE | ID: mdl-26844684

RESUMO

As two-dimensional (2D) nanomaterials, graphene (G) and graphene oxide (GO) have evolved into new platforms for biomedical research as biosensors, imaging agents, and drug delivery carriers. In particular, the unique surface properties of GO can be an important tool in modulating cellular behavior and various biological sequences. Here, we report that a composite system of graphene oxide/polypeptide thermogel (GO/P), prepared by temperature-sensitive sol-to-gel transition of a GO-suspended poly(ethylene glycol)-poly(L-alanine) (PEG-PA) aqueous solution significantly enhances the expression of adipogenic biomarkers, including PPAR-γ, CEBP-α, LPL, AP2, ELOVL3, and HSL, compared to both a pure hydrogel system and a composite system of G/P, graphene-incorporated hydrogel. We prove that insulin, an adipogenic differentiation factor, preferentially adhered to GO, is supplied to the incorporated stem cells in a sustained manner over the three-dimensional (3D) cell culture period. On the other hand, insulin is partially denatured in the presence of G and interferes with the adipogenic differentiation of the stem cells. The study suggests that a 2D/3D composite system is a promising platform as a 3D cell culture matrix, where the surface properties of 2D materials in modulating the fates of the stem cells are effectively transcribed in a 3D culture system.


Assuntos
Adipogenia/efeitos dos fármacos , Diferenciação Celular/efeitos dos fármacos , Grafite/farmacologia , Células-Tronco Mesenquimais/efeitos dos fármacos , Engenharia Tecidual , Alanina/química , Técnicas de Cultura de Células , Portadores de Fármacos/química , Regulação da Expressão Gênica no Desenvolvimento/efeitos dos fármacos , Grafite/química , Humanos , Óxidos/química , Tonsila Palatina/citologia , Peptídeos/química , Polietilenoglicóis/química
10.
Adv Healthc Mater ; 5(3): 353-63, 2016 Feb 04.
Artigo em Inglês | MEDLINE | ID: mdl-26634888

RESUMO

Injectable inorganic/organic composite systems consisting of well-defined mesocrystals (4-8 µm) of calcium phosphate and polypeptide thermogel significantly enhance the osteogenic differentiation of the tonsil derived mesenchymal stem cells (TMSCs). Compared to composite systems incorporating nanoparticles (10-100 nm) or pure hydrogel systems, osteogenic biomarkers including alkaline phosphatase (ALP), bone morphogenetic protein 2, and osteocalcin are highly expressed at both the mRNA level and the protein level in the mesocrystal composite systems. ALP activity of differentiated cells is also significantly higher in the mesocomposite systems compared to the nanocomposite systems or the pure hydrogel systems. The mesocomposite systems provide not only hard surfaces for binding the cells/proteins by the inorganic mesocrystals but also a soft matrix for holding the cells by the hydrogel. Through the current research, (1) a novel method of preparing mesocrystals is developed, (2) TMSCs are proved as a new resource of stem cells, and (3) the mesocomposite systems are proved to be a promising tool in controlling stem cell differentiation. (4) Finally, the research emphasizes the significance of mesoscience as a new perspective of science in controlling cell and material interfaces.


Assuntos
Diferenciação Celular/efeitos dos fármacos , Células-Tronco Mesenquimais/efeitos dos fármacos , Nanocompostos/administração & dosagem , Nanocompostos/química , Osteogênese/efeitos dos fármacos , Tonsila Palatina/efeitos dos fármacos , Fosfatase Alcalina/metabolismo , Proteína Morfogenética Óssea 2/metabolismo , Fosfatos de Cálcio/metabolismo , Técnicas de Cultura de Células/métodos , Células Cultivadas , Criança , Feminino , Humanos , Células-Tronco Mesenquimais/metabolismo , Osteocalcina/metabolismo , Tonsila Palatina/metabolismo , Células-Tronco/efeitos dos fármacos
11.
Biomacromolecules ; 16(12): 3853-62, 2015 Dec 14.
Artigo em Inglês | MEDLINE | ID: mdl-26551029

RESUMO

Zwitterionic polymers have been investigated as surface-coating materials due to their low protein adsorption properties, which reduce immunogenicity, biofouling, and bacterial adsorption of coated materials. Most zwitterionic polymers, reported so far, are based on (meth)acrylate polymers which can induce toxicity by residual monomers or amines produced by degradation. Here, we report a new zwitterionic polymer consisting of phosphorylcholine (PC) and biocompatible poly(propylene glycol) (PPG) as a new thermogelling material. The PC-PPG-PC polymer aqueous solution undergoes unique multiple sol-gel transitions as the temperature increases. A heat-induced unimer-to-micelle transition, changes in ionic interactions, and dehydration of PPG are involved in the sol-gel transitions. Based on the broad gel window and low protein adsorption properties, the PC-PPG-PC thermogel is proved for sustained delivery of protein drugs and stem cells over 1 week.


Assuntos
Acrilatos/química , Materiais Biocompatíveis/síntese química , Preparações de Ação Retardada/síntese química , Fosforilcolina/química , Polímeros/química , Propilenoglicóis/química , Animais , Materiais Biocompatíveis/farmacologia , Sobrevivência Celular/efeitos dos fármacos , Criança , Preparações de Ação Retardada/farmacologia , Diabetes Mellitus Experimental/sangue , Diabetes Mellitus Experimental/tratamento farmacológico , Diabetes Mellitus Experimental/patologia , Composição de Medicamentos/métodos , Liberação Controlada de Fármacos , Feminino , Géis , Humanos , Hipoglicemiantes/química , Hipoglicemiantes/farmacologia , Insulina/química , Insulina/farmacologia , Masculino , Células-Tronco Mesenquimais/citologia , Células-Tronco Mesenquimais/efeitos dos fármacos , Tonsila Palatina/citologia , Tonsila Palatina/efeitos dos fármacos , Transição de Fase , Polimerização , Cultura Primária de Células , Ratos , Ratos Sprague-Dawley , Temperatura Ambiente
12.
Adv Healthc Mater ; 4(10): 1565-74, 2015 Jul 15.
Artigo em Inglês | MEDLINE | ID: mdl-26033880

RESUMO

Neuronal differentiation of tonsil-derived mesenchymal stem cells (TMSCs) is investigated in a 3D hybrid system. The hybrid system is prepared by increasing the temperature of poly(ethylene glycol)-poly(l-alanine) aqueous solution to 37 °C through the heat-induced sol-to-gel transition, in which TMSCs and growth factor releasing microspheres are suspended. The in situ formed gel exhibits a modulus of 800 Pa at 37 °C, similar to that of brain tissue, and it is robust enough to hold the microspheres and cells during the 3D culture of TMSCs. The neuronal growth factors are released over 12-18 d, and the TMSCs in a spherical shape initially undergo multipolar elongation during the 3D culture. Significantly higher expressions of the neuronal biomarkers such as nuclear receptor related protein (Nurr-1), neuron specific enolase, microtubule associated protein-2, neurofilament-M, and glial fibrillary acidic protein are observed in both mRNA level and protein level in the hybrid systems than in the control experiments. This study proves the significance of a controlled drug delivery concept in tissue engineering or regenerative medicine, and a 3D hybrid system with controlled release of growth factors from microspheres in a thermogel can be a very promising tool.


Assuntos
Géis/química , Células-Tronco Mesenquimais/citologia , Microesferas , Neurônios/citologia , Tonsila Palatina/citologia , Diferenciação Celular , Células Cultivadas , Criança , Feminino , Proteína Glial Fibrilar Ácida/genética , Proteína Glial Fibrilar Ácida/metabolismo , Humanos , Células-Tronco Mesenquimais/metabolismo , Microscopia de Fluorescência , Proteínas Associadas aos Microtúbulos/genética , Proteínas Associadas aos Microtúbulos/metabolismo , Proteínas de Neurofilamentos/genética , Proteínas de Neurofilamentos/metabolismo , Neurônios/metabolismo , Membro 2 do Grupo A da Subfamília 4 de Receptores Nucleares/genética , Membro 2 do Grupo A da Subfamília 4 de Receptores Nucleares/metabolismo , Peptídeos/química , Polietilenoglicóis/química , RNA Mensageiro/metabolismo , Medicina Regenerativa , Temperatura Ambiente , Engenharia Tecidual
13.
Mater Sci Eng C Mater Biol Appl ; 33(3): 1662-70, 2013 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-23827621

RESUMO

Poly(vinyl chloride) (PVC) membrane was hydrophilically modified by grafting with poly(oxyethylene methacrylate) (POEM) using atom transfer radical polymerization (ATRP). The successful grafting of PVC main chain by POEM was characterized by Fourier transform infrared spectroscopy (FT-IR). The molecular weight and hydrophilicity of membranes increased with the amount of POEM grafting, as characterized by gel permeation chromatography (GPC) and contact angle measurement, respectively. Transmission electron microscope (TEM) and small angle X-ray scattering (SAXS) analysis revealed the microphase-separated structure of PVC-g-POEM and the domain spacing increased from 59.3 to 86.1 nm with increasing grafting degree. Scanning electron microscopy (SEM) was used for the direct visualization of the mouse embryonic fibroblast (MEF) cell and bacteria adhesion on the membrane surface. Protein adsorption and eukaryotic and prokaryotic cell adhesion tests showed that the bioinert properties of membranes were significantly increased with POEM content.


Assuntos
Materiais Biocompatíveis/farmacologia , Membranas Artificiais , Polímeros/farmacologia , Ácidos Polimetacrílicos/farmacologia , Polivinil/farmacologia , Tensoativos/farmacologia , Animais , Bacillus cereus/citologia , Bacillus cereus/efeitos dos fármacos , Bacillus cereus/ultraestrutura , Aderência Bacteriana/efeitos dos fármacos , Cromatografia em Gel , Escherichia coli/citologia , Escherichia coli/efeitos dos fármacos , Escherichia coli/ultraestrutura , Fibroblastos/citologia , Fibroblastos/efeitos dos fármacos , Camundongos , Peso Molecular , Polímeros/química , Ácidos Polimetacrílicos/química , Polivinil/química , Espalhamento a Baixo Ângulo , Espectroscopia de Infravermelho com Transformada de Fourier , Staphylococcus aureus/citologia , Staphylococcus aureus/efeitos dos fármacos , Staphylococcus aureus/ultraestrutura , Difração de Raios X
14.
Bioresour Technol ; 123: 558-65, 2012 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-22944490

RESUMO

Hydrogen can be produced by catalytic steam reforming (CSR) of biomass-derived oil. Typically bio oil contains 12-14% acetic acid; therefore, this acid was chosen as model compound for reforming of biooil with the help of a Cu-Zn/Ca-Al catalyst for high yield of H(2) with low CH(4) and CO content. Calcium aluminate support was prepared by solid-solid reaction at 1350°C. X-ray diffraction indicates 12CaO·7Al(2)O(3) as major, CaA(l4)O(7) and Ca(5)A(l6)O(14) as minor phases. Cu and Zn were loaded onto the support by wet-impregnation at 10 and 1wt.%, respectively. The catalysts were characterized by Brunauer-Emmett-Teller (BET), Scanning Electron Microscopy (SEM) and Transmission Electron Microscopy TEM and the surface area for both support and Cu-Zn were 10.5 and 5.8m(2)/g, respectively. CSR was carried out in a tubular fixed bed reactor (I.D.=19mm) at temperatures between 600 and 800°C with 3-g loadings and (H(2)O/acetic acid) wt. ratio of 9:1. Significantly high (80%) yield of hydrogen was obtained over Cu-Zn/Ca-Al catalyst, as incorporation of Zn enhanced the H(2) yield by reducing deactivation of the catalyst. The coke formation on the support (Ca-12/Al-7) surface was negligible due to the presence of excess oxygen in the 12CaO·7Al(2)O(3) phase.


Assuntos
Ácido Acético/química , Compostos de Alumínio/química , Biotecnologia/métodos , Compostos de Cálcio/química , Cobre/química , Hidrogênio/metabolismo , Vapor , Zinco/química , Catálise , Tamanho da Partícula , Temperatura Ambiente , Fatores de Tempo , Difração de Raios X
15.
Hemoglobin ; 34(6): 604-9, 2010.
Artigo em Inglês | MEDLINE | ID: mdl-21077771

RESUMO

Fetal hemoglobin (Hb F) is the most studied modifier of sickle cell disease. Coinheritance of high Hb F determinants such as δß-thalassemia (δß-thal) and hereditary persistence of fetal hemoglobin (HPFH) can contribute to raised Hb F concentration in these patients. One hundred and seventy-six cases of sickle cell disease with high Hb F were screened for the presence of the Asian Indian deletion-inversion (G)γ((A)γδß)°-thal and HPFH-3 (Indian, 48.5 kb) disorders. Three cases from two unrelated families were found to have sickle cell disease and the ((A)γδß)°-thal genotype. Three other members had heterozygous (G)γ((A)γδß)°-thal. None had HPFH-3. Despite very high Hb F concentrations and linkage of the ß(S) gene to Asian haplotypes, the compound heterozygotes had severe clinical presentation, possibly because of heterocellular distribution of Hb F. In conclusion, these high Hb F determinants are not common causes of high Hb F in Indian sickle cell disease patients.


Assuntos
Anemia Falciforme/genética , Hemoglobina Fetal/genética , Talassemia beta/genética , Talassemia delta/genética , Adolescente , Anemia Falciforme/patologia , Criança , Feminino , Estudos de Associação Genética , Heterozigoto , Humanos , Índia , Masculino , Mutação , Adulto Jovem , Talassemia beta/patologia , Talassemia delta/patologia
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