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J Chromatogr Sci ; 50(8): 666-72, 2012 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-22595260


This paper describes the determination of fatty acid composition of coffee, citrus and rum distillery wastes using reversed-phase high-performance liquid chromatography (RP-HPLC). Lipid extracts of the waste samples are derivatized with phenacyl bromide and their phenacyl esters are separated on a C8 reversed-phase column by using continuous gradient elution with water and acetonitrile. The presence of saturated and unsaturated fatty acids in quantifiable amounts in the examined wastes, as well as the high percentage recoveries, are clear indications that these wastes have potential value as inexpensive sources of lipids. The HPLC procedures described here could be adopted for further analysis of materials of this nature.

Acetofenonas/química , Agricultura , Cromatografia Líquida de Alta Pressão/instrumentação , Cromatografia Líquida de Alta Pressão/métodos , Ácidos Graxos/análise , Resíduos Industriais/análise , Bebidas Alcoólicas , Citrus , Café , Jamaica
J Chromatogr Sci ; 47(8): 674-80, 2009 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-19772744


Jamaican agro-industries generate large quantities of wastes, which are either discarded or under-utilized. In order to evaluate the possible utilization of these wastes, it is necessary that the profiles of the major biochemical groups be developed. This paper describes the determination of the amino acid composition of coffee, citrus, and rum distillery wastes using a reversed-phase high-performance liquid chromatography method. Acid hydrolysates of the wastes are derivatized with phenylisothiocyanate. They are analyzed as their phenylthiocarbamyl derivatives and determined quantitatively using norleucine as the internal standard. The presence of all the 17 amino acids investigated, nine of which include those essential for animal nutrition, are observed in the samples investigated, suggesting a high quality of protein with implications in the formulation of animal feeds.

Agricultura , Aminoácidos/análise , Aminoácidos/química , Cromatografia Líquida de Alta Pressão/métodos , Resíduos Industriais/análise , Isotiocianatos/química , Jamaica