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1.
Hua Xi Kou Qiang Yi Xue Za Zhi ; 38(3): 263-269, 2020 Jun 01.
Artigo em Chinês | MEDLINE | ID: mdl-32573132

RESUMO

OBJECTIVE: To investigate the effects of preparation height and cement space on the fit and retention of computer aided design (CAD)/computer aided manufacturing (CAM) zirconia crown, and to provide reference for the clinical design and fabrication of CAD/CAM crowns. METHODS: 3D printing system was used to fabricate resin abutment teeth with convergence angle of 2° and height of 1-3 mm. The models' optical impressions were collected by the three-shape scanner. Then, the cement spaces were set by Cradle CAD/CAM system at 10-50 µm to create an all-ceramic zirconia crown. The fit of the crowns was measured by using silicone rubber interstitial impression method. The retention of the crowns was measured by pull-off test with uniaxial tensile force after the crown was bonded. The data were analyzed by SPSS 22.0 soft-ware. RESULTS: When the preparation height was fixed, the fitness values of different cement space groups have statistical difference (P<0.05), whereas the retention values of different cement space groups have no statistical difference (P>0.05). The fitness values of different preparation height groups have no statistical difference (P>0.05), and the retention values of different preparation height groups have statistical difference (P<0.05) when the cement space was fixed. No interaction was observed between the cement space and the preparation height (P>0.05). CONCLUSIONS: When cradle CAD/CAM system is used to create a full crown in the clinic, the preparation height should be set to more than 3 mm, and the cement space should be set at 30 µm.


Assuntos
Coroas , Planejamento de Prótese Dentária , Desenho Assistido por Computador , Porcelana Dentária , Zircônio
2.
Oral Health Prev Dent ; 18(3): 485-492, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32515419

RESUMO

PURPOSE: The present study aimed to assess the current situation of Chinese dental bachelor interns on HIV/AIDS-related knowledge and their attitudes towards HIV/AIDS patients. MATERIALS AND METHODS: A cross-sectional, paper-based survey involving 147 dental students from three Chinese dental schools was conducted. Students were recruited to complete the questionnaire regarding their knowledge, awareness and attitudes concerning HIV/AIDS anonymously and voluntarily. RESULTS: A total of 144 students responded to the study, generating a response rate of 98.0%. Although 97.0% of the dental students believed dentists were at high risk of HIV infection, 97.2% of students expressed no prejudice towards HIV/AIDS patients and stated their willingness to provide oral healthcare service for such patients. No statistically significant differences in the responses on attitudes and some basic knowledge were found between students who had received the relevant education about infection control and the students who had not. However, regarding most questions about oral manifestations in adult and paediatric patients living with AIDS, the students who received relevant education showed more knowledge than the students who did not (p <0.05). The cognitive level about respecting HIV/AIDS patients' autonomy and privacy was generally low in all the students. CONCLUSIONS: Most of the dental students in this survey held positive attitudes towards HIV/AIDS patients and good grasp of some basic knowledge about HIV/AIDS. On the other hand, the questionnaire results reflected gaps in education, such as respecting HIV/AIDS patients' privacy.


Assuntos
Síndrome de Imunodeficiência Adquirida , Assistência Odontológica para Doentes Crônicos , Infecções por HIV , Adulto , Atitude do Pessoal de Saúde , Criança , Estudos Transversais , HIV , Conhecimentos, Atitudes e Prática em Saúde , Humanos , Inquéritos e Questionários
3.
Acta Biomater ; 2020 May 27.
Artigo em Inglês | MEDLINE | ID: mdl-32470526

RESUMO

Extracellular matrix (ECM) remodeling is essential for the development and functions of connective tissues (e.g., heart, muscle and the periodontal ligament), and entails the highly anisotropic response of cells and their organized ECM molecules to mechanical stimulation. However, the nature of how cells remodel their surrounding ECM under mechanical stimulation remains elusive. Here, we encapsulated human periodontal ligament stem cells (hPDLSCs) within an aligned rat collagen scaffold labeled with fluorescein isothiocyanate (FITC) and applied mechanical stimulation on the scaffold using magnetic stretching. Through tracking the FITC-labeled rat collagen scaffold and the newly secreted human type I collagen, we studied the effect of magnetic stretching on the mechanism of aligned ECM remodeling by the encapsulated cells. We found that the aligned topography combined with magnetic stretching could significantly promote initial ECM degradation and new ECM secretion: expression of matrix metalloproteinases 1 and 9 is increased markedly, and the elastic modulus of the stretched scaffold (75 kPa) is significantly higher than that of the random scaffold (50 kPa). The data support a model whereby the cells remodel their surrounding ECM under continuous stretching through degradation and then secretion of new ECM to integrate with the aligned ECM and maintain tissue function. Our study offers a valuable basis for future optimized design of biomaterial scaffolds for clinical translation. STATEMENT OF SIGNIFICANCE: Extracellular matrix (ECM) remodeling is essential for the development and functions of connective tissues. However, the nature of how cells remodel their surrounding aligned ECM under mechanical stimulation remains elusive. Herein, we developed a method to reveal the remodeling of aligned rat collagen scaffold by the encapsulated human periodontal ligament stem cells (hPDLSCs) using fluorescence imaging. We found that the aligned topography combined with magnetic stretching could significantly promote initial ECM degradation and new ECM secretion: the expression of matrix metalloproteinase 1 and 9 are significantly higher, and the elastic modulus increases from 50 kPa to 75 kPa as compared to the random collagen scaffold encapsulating hPDLSCs. Our study holds great potential in optimization of bio-scaffold design for clinical translation.

4.
ACS Appl Mater Interfaces ; 12(23): 26432-26443, 2020 Jun 10.
Artigo em Inglês | MEDLINE | ID: mdl-32429664

RESUMO

The development of a highly effective photosensitizer (PS) that can be activated with a low-power single light is a pressing issue. Herein, we report a PS for synergistic photodynamic and photothermal therapy constructed through self-assembly of poly(selenoviologen) on the surface of core-shell NaYF4:Yb/Tm@NaYF4 upconversion nanoparticles. The hybrid UCNPs/PSeV PS showed strong ROS generation ability and high photothermal conversion efficiency (∼52.5%) under the mildest reported-to-date irradiation conditions (λ = 980 nm, 150 mW/cm2, 4 min), leading to a high efficiency in killing methicillin-resistant Staphylococcus aureus (MRSA) both in vitro and in vivo. Remarkably, after intravenous injection, the reported PS accumulated preferentially in deep MRSA-infected tissues and achieved an excellent therapeutic index. This PS design realizes a low-power single-NIR light-triggered synergistic phototherapy and provides a simple and versatile strategy to develop safe clinically translatable agents for efficient treatment of deep tissue bacterial inflammations.

5.
J Evid Based Dent Pract ; 20(1): 101398, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-32381407

RESUMO

OBJECTIVE: To evaluate the additional effect of probiotic Lactobacillus in the nonsurgical management of peri-implant diseases (peri-implant mucositis and peri-implantitis). METHODS: Six databases were searched up to May 2019 without time and language restrictions. Study selection and data extraction were conducted independently by 2 reviewers. The inclusion criteria for this systematic review were defined based on the participants, intervention, comparison, outcomes, and study design (PICOS) format. Randomized controlled trials comparing nonsurgical treatment combined with probiotic Lactobacillus or placebo agent in patients with peri-implant diseases were included. The methodological quality of retrieved studies was assessed according to the Cochrane Collaboration's Risk of Bias tool, and the quality of evidence was evaluated using the Grading of Recommendations Assessment, Development and Evaluation (GRADE) tool. Odds ratio and 95% confidence interval (CI) were used to describe dichotomous data, while mean difference and standardized mean difference with 95% CI were used to describe continuous variables. RESULTS: Seven randomized controlled trials with 296 implants were included in this meta-analysis. The mean difference of probing pocket depth (PPD) was -0.05 (95% CI: -0.28 to 0.18; P = .67) immediately after treatment termination and -0.17 (95% CI: -1.01 to 0.67, P = .69) at least 2 months after treatment termination. There was a slight reduction of PPD after treatment termination. Compared with placebo, Lactobacillus provided limited benefits in peri-implant mucositis. There were no significant differences in the secondary outcomes of bleeding on probing or plaque index (P > .05). In a narrative synthesis of peri-implantitis, the effect of Lactobacillus on PPD and bleeding on probing remained controversial. CONCLUSIONS: This systematic review and meta-analysis showed that probiotic Lactobacillus provide limited benefits to the nonsurgical treatment of peri-implant mucositis or peri-implantitis.


Assuntos
Implantes Dentários , Peri-Implantite , Probióticos , Estomatite , Humanos , Lactobacillus
6.
ACS Appl Mater Interfaces ; 12(16): 18385-18394, 2020 Apr 22.
Artigo em Inglês | MEDLINE | ID: mdl-32212618

RESUMO

The development of novel applications of ultralong organic phosphorescent (UOP) materials is highly desired. Herein, a series of UOP materials (EDCz, E = O, S, Se, and Te) for bacterial afterglow imaging and photodynamic therapy (PDT) is reported. By structurally bonding with the chalcogen atoms with π-conjugated scaffolds, EDCz not only absorbs visible light but also emits UOP with an efficiency of ca. 0.01-6.8% and a long lifetime of 0.08-0.318 s under ambient conditions. Benefiting from the long-lived triplet excited states, the SeDCz nanocrystals (NCs) possessed the best optical properties in the series, generating 1O2 under white light irradiation and performing as an agent for Staphylococcus aureus afterglow imaging and PDT at a low concentration (98 ng mL-1). The SeDCz NCs are also utilized as real-time UOP imaging agents and promoted healing of infected wounds in living mice. To the best of our knowledge, this study presents the first example of UOP-based bacterial photodynamic theranostic agents and creates a platform for the next-generation efficient UOP-based photosensitizers for bioimaging and skin regeneration.

7.
J Prosthet Dent ; 2020 Feb 06.
Artigo em Inglês | MEDLINE | ID: mdl-32037295

RESUMO

STATEMENT OF PROBLEM: Tooth preparations for ceramic crowns require precision and accuracy, which may be influenced by the choice of dental handpiece. However, comparisons of the accuracy of tooth preparations made with traditional air-turbine handpieces and electric handpieces are lacking. PURPOSE: The purpose of this in vitro study was to evaluate operator preferences and tooth preparation performance by using electric and air-turbine handpieces with self-reported preferences, sound levels, surface roughness, and the fit of the crown produced. MATERIAL AND METHODS: Twenty dentists were asked to use the air-turbine or the electric handpiece. Feedback on the noise, weight, feel of grip, flexibility, and tooth preparation in general was scored according to a visual analog scale (VAS). Additionally, the dentists were asked to complete a questionnaire on their handpiece preference. The noise of the 2 handpieces was measured by using a precision sound level meter. The surface roughness of 10 teeth was measured by using a profilometer. The other 18 teeth were prepared to measure the marginal and internal fit of ceramic crowns by the replica technique. The VAS scores of operator preferences were analyzed with the Wilcoxon signed ranks test. Decibel levels were analyzed with the Mann-Whitney U test. The McNemar test was used to compare the ratio of preferred handpiece. The surface roughness and marginal and internal fit were analyzed with the independent t test to determine significant differences (all α=.05). RESULTS: The electric handpiece was heavier, had a poorer grip feel, was less flexible (P<.001), produced lower noise and better feeling of the tooth preparation in general (P<.001), and was preferred in the finishing stage for its greater smoothness (P<.05). The noise produced by the electric handpiece was lower during both idling and tooth preparation at 15-cm, 30-cm, and 45-cm distances (P<.01). The electric handpiece produced surface roughness values (Sa) similar to those of the air-turbine handpiece (P>.05). No significant differences were noted for the marginal and internal crown fit between the air-turbine handpiece and electric handpiece groups (P>.05). CONCLUSIONS: Despite its heavier weight, poorer grip feel, and less flexibility, the electric handpiece emitted lower noise, produced better feeling of the tooth preparation in general, and was preferred in the finishing step of tooth preparation for its greater smoothness than the air-turbine handpiece. The surface roughness of the prepared teeth and the crown fit between the tooth and ceramic crown were not affected by the air-turbine or electric handpiece.

8.
BMC Oral Health ; 20(1): 7, 2020 Jan 06.
Artigo em Inglês | MEDLINE | ID: mdl-31907038

RESUMO

BACKGROUND: Appropriate mechanical stimulation is essential for bone homeostasis in healthy periodontal tissues. While the osteogenesis and osteoclast differentiation of inflammatory periodontal ligament cells under different dynamic loading has not been yet clear. The aim of this study is to clarify the inflammatory, osteogenic and pro-osteoclastic effects of different cyclic stress loading on the inflammatory human periodontal ligament cells (hPDLCs). METHODS: hPDLCs were isolated from healthy premolars and cultured in alpha minimum Eagle's medium (α-MEM). Lipopolysaccharides (LPS) were used to induce the inflammation state of hPDLCs in vitro. Determination of LPS concentration for the model of inflammatory periodontium was based on MTT and genes expression analysis. Then the cyclic stress of 0, 0-50, 0-90 and 0-150 kPa was applied to the inflammatory hPDLCs for 5 days respectively. mRNA and protein levels of osteogenic, osteoclastic and inflammation-related markers were examined after the treatment. RESULTS: MTT and RT-PCR results showed that 10 µg/ml LPS up-regulated TNF-α, IL-1ß, IL-6, IL-8 and MCP-1 mRNA levels (P < 0.05) and did not affect the cell viability (P > 0.05). The excessive loading of stress (150 kPa) with or without LPS strongly increased the expression of inflammatory-related markers TNF-α, IL-1ß, IL-6, IL-8, MCP-1 (P < 0.05) and osteoclastic markers RANKL, M-CSF, PTHLH and CTSK compared with other groups (P < 0.05), but had no significant effect on osteogenic genes. While 0-90 kPa cyclic pressure could up-regulate the expression of osteogenic genes ALP, COL-1, RUNX2, OCN, OPN and OSX in the healthy hPDLSCs. CONCLUSIONS: Collectively, it could be concluded that 0-150 kPa was an excessive stress loading which accelerated both inflammatory and osteoclastic effects, while 0-90 kPa may be a positive factor for the osteogenic differentiation of hPDLCs in vitro.

9.
J Periodontal Res ; 55(3): 381-391, 2020 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-31854466

RESUMO

BACKGROUND AND OBJECTIVE: Oxidative stress has been suggested as an important pathogenic factor contributing to chronic periodontitis with diabetes mellitus (CPDM). Previous studies have revealed the potential therapeutic properties of baicalein (BCI) in oxidative stress-related diseases; however, the antioxidant effects of BCI on therapy for individual with CPDM remain largely unexplored. Nuclear factor erythroid 2-related factor 2 (Nrf2) plays a critical role in cellular defence against oxidative stress. In this study, we aim to determine whether BCI prevents diabetes-related periodontal tissue destruction by regulating Nrf2 signaling pathway. MATERIAL AND METHODS: Human gingival epithelial cells (hGECs) were challenged with high glucose (HG, 25 mmol/L) and/or lipopolysaccharide (LPS, 20 µg/mL). Reactive oxygen species (ROS) were detected by fluorescence-activated cell sorting. The changes of antioxidant-related genes, including Nrf2, catalase (Cat), glutamate-cysteine ligase catalytic subunit (Gclc), superoxide dismutase 1 (Sod1), and superoxide dismutase 2 (Sod2), were quantified by real-time PCR. The localization of phospho-Nrf2 (pNrf2, S40) in the nucleus was detected by immunofluorescence staining and laser scanning confocal microscope (LSCM). PNrf2 and total form of Nrf2 were determined using western blot. The above indicators together with mitochondrial membrane potential (MMP) were further investigated in hGECs pre-treated with different concentrations of BCI (0.01, 0.1, or 0.5 µg/mL) before stimulated with HG plus LPS (GP). Finally, the role of BCI in activating Nrf2 signaling pathway and relieving the alveolar bone absorption was examined in the CPDM model of Sprague Dawley rats. CPDM rats were oral gavaged with BCI (50, 100, or 200 mg/kg daily). The pNrf2 was detected by immunohistochemistry, and the alveolar bone absorption was examined by microcomputed tomography. RESULTS: Our results showed that ROS were significantly increased in both groups of HG and LPS, with the strongest generation in the GP group. In terms of ROS-related gene expression, we found that the mRNA levels of Nrf2, Cat, Gclc, Sod1, and Sod2 were significantly decreased in HG and LPS groups. In consistent with the strongest induction of ROS in GP group, the gene expression in GP group was further decreased as compared to those of HG and LPS groups. Also, the expression of pNrf2 exhibited the same trend with the expression of those antioxidant genes. However, the generation of ROS and the loss of mitochondrial membrane potential induced by GP were abolished by pre-treatment with different concentrations of BCI (0.01, 0.1, or 0.5 µg/mL). Interestingly, we observed that BCI promoted the nucleus translocation of pNrf2, as well as the gene expression levels of pNrf2 and its target genes (Cat, Gclc, Sod1, and Sod2). Finally, in the CPDM animal model, we found that BCI (at concentrations: 50, 100, and 200 mg/kg) markedly increased the number of pNrf2-positive cells in periodontal tissue and mitigated the alveolar bone loss. CONCLUSIONS: Our data revealed a potential role for clinic application of BCI under CPDM conditions, suggesting a new therapeutic drug for CPDM patients.

10.
BMC Oral Health ; 19(1): 73, 2019 05 02.
Artigo em Inglês | MEDLINE | ID: mdl-31046751

RESUMO

BACKGROUND: Epigallocatechin-3-gallate (EGCG) was recently proposed to have the potential to regulate bone metabolism, however, its influence on osteogenesis remains controversial. The present study aimed to investigate the effects of EGCG on the proliferation and osteogenesis of human periodontal ligament cells (hPDLCs). METHODS: Cells were cultured in osteogenic medium and treated with EGCG at various concentrations. Cell proliferation was analyzed using a CCK-8 assay and acridine orange (AO)/ethidium bromide (EB) staining. Flow cytometry was used to measure the intracellular reactive oxygen species (ROS) potential of hPDLCs. The expression levels of osteogenic marker genes and proteins in hPDLCs, including type I collagen (COL1), runt-related transcription factor 2 (RUNX2), osteopontin (OPN), and osterix (OSX), were determined by quantitative real-time polymerase chain reaction (qRT-PCR) and western blot analysis. In addition, alkaline phosphatase (ALP) activity was monitored both quantitatively and qualitatively. Extracellular matrix mineralization was further analyzed by alizarin red S staining. RESULTS: The results showed that EGCG concentrations from 6 to 10 µM increased the ROS level and inhibited the cell proliferation of hPDLCs. EGCG concentrations from 2 to 8 µM effectively increased extracellular matrix mineralization, in which 4 and 6 µM EGCG generated the most mineralizing nodules. The ALP activity and the mRNA and protein expression levels of the tested osteogenic markers were most strongly up-regulated by treatment with 4 and 6 µM EGCG. CONCLUSIONS: The present study demonstrated that EGCG might promote the osteogenesis of hPDLCs in a dose-dependent manner, with concentrations of 4 and 6 µM EGCG showing the strongest osteogenic enhancement without cytotoxicity, indicating a promising role for EGCG in periodontal regeneration in patients with deficient alveolar bone in the future.


Assuntos
Catequina/análogos & derivados , Diferenciação Celular , Proliferação de Células , Osteogênese , Ligamento Periodontal , Fosfatase Alcalina , Células Cultivadas , Humanos
11.
J Mech Behav Biomed Mater ; 91: 38-44, 2019 03.
Artigo em Inglês | MEDLINE | ID: mdl-30529985

RESUMO

OBJECTIVES: To evaluate the dentinal tubular occlusion of nano-hydroxyapatite (nHAp) containing desensitizing toothpastes and their influence on the resin-dentin bonding performance of two mild self-etch adhesives. MATERIALS AND METHODS: Mid-coronal dentin specimens were prepared from obtained intact human third molars. They were immersed in 1% citric acid for 20 s to expose the dentinal tubules to simulate sensitive teeth and then randomly divided into four groups. The control group received no desensitizing treatment. Experimental groups were treated with two commercial nHAp containing desensitizing toothpastes (Biorepair and Dontodent) and an experimental pure nHAp paste respectively. Each group was further divided into two subgroups and bonded with either G-Bond or Clearfil S3 Bond. The micro-tensile bond strength was tested and failure mode distribution was analyzed. Moreover, the effect of desensitizers on dentinal tubular occlusion was observed by the field-emission scanning electron microscope (FESEM). Resin infiltration of the adhesives labeled by fluorescent Rhodamin B was evaluated under confocal laser scanning microscopy (CLSM). RESULTS: FESEM revealed that all the desensitizers noticeably occluded the dentinal tubules, and the extents were completer after application for 7 days. The majority of the occlusion still preserved even after acid challenge with cola or adhesive. CLSM demonstrated shorter resin tags were produced in the desensitized groups. When bonding with G-Bond, the pure nHAp group showed comparable bond strength to the control group, while Biorepair and Dontodent treatment decreased the bond strength. For groups bonded with Clearfil S3 Bond, all the desensitizers reduced the bond strengths compared to the control and no significant difference was found among the three groups. CONCLUSION: Nano-hydroxyapatite containing desensitizing toothpastes could occlude dentinal tubules effectively with a certain degree of acid resistance, which contributes to the relief of dentin hypersensitivity. While, the application of these nHAp desensitizers comprised the resin infiltration of G-Bond and Clearfil S3 Bond, resulting in decreased bond strengths of the resin-dentin bonding.

12.
Adv Sci (Weinh) ; 5(10): 1800873, 2018 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-30356983

RESUMO

Biomineralization in vertebrates is initiated via amorphous calcium phosphate (ACP) precursors. These precursors infiltrate the extracellular collagen matrix where they undergo phase transformation into intrafibrillar carbonated apatite. Although it is well established that ACP precursors are released from intracellular vesicles through exocytosis, an unsolved enigma in this cell-mediated mineralization process is how ACP precursors, initially produced in the mitochondria, are translocated to the intracellular vesicles. The present study proposes that mitophagy provides the mechanism for transfer of ACP precursors from the dysfunctioned mitochondria to autophagosomes, which, upon fusion with lysosomes, become autolysosomes where the mitochondrial ACP precursors coalesce to form larger intravesicular granules, prior to their release into the extracellular matrix. Apart from endowing the mitochondria with the function of ACP delivery through mitophagy, the present results indicate that mitophagy, triggered upon intramitochondrial ACP accumulation in osteogenic lineage-committed mesenchymal stem cells, participates in the biomineralization process through the BMP/Smad signaling pathway.

13.
Sci Rep ; 8(1): 2848, 2018 02 12.
Artigo em Inglês | MEDLINE | ID: mdl-29434321

RESUMO

Coptidis Rhizoma binds to the membrane receptors on hPDLSC/CMC, and the active ingredient Berberine (BER) that can be extracted from it may promote the proliferation and osteogenesis of periodontal ligament stem cells (hPDLSC). The membrane receptor that binds with BER on the cell surface of hPDLSC, the mechanism of direct interaction between BER and hPDLSC, and the related signal pathway are not yet clear. In this research, EGFR was screened as the affinity membrane receptor between BER and hPDLSC, through retention on CMC, competition with BER and by using a molecular docking simulation score. At the same time, the MAPK PCR Array was selected to screen the target genes that changed when hPDLSC was simulated by BER. In conclusion, BER may bind to EGFR on the cell membrane of hPDLSC so the intracellular ERK signalling pathways activate, and nuclear-related genes of FOS change, resulting in the effect of osteogenesis on PDLSC.


Assuntos
Berberina/farmacologia , Sistema de Sinalização das MAP Quinases/efeitos dos fármacos , Osteogênese , Ligamento Periodontal/citologia , Berberina/química , Diferenciação Celular/efeitos dos fármacos , Células Cultivadas , Receptores ErbB/química , Receptores ErbB/genética , Receptores ErbB/metabolismo , Humanos , Modelos Moleculares , Simulação de Acoplamento Molecular , Ligamento Periodontal/efeitos dos fármacos , Ligamento Periodontal/metabolismo , Proteínas Proto-Oncogênicas c-fos/genética , Proteínas Proto-Oncogênicas c-fos/metabolismo , Células-Tronco/citologia , Células-Tronco/efeitos dos fármacos , Células-Tronco/metabolismo
14.
Int J Med Inform ; 102: 150-155, 2017 06.
Artigo em Inglês | MEDLINE | ID: mdl-28495343

RESUMO

INTRODUCTION: Proper cooperation between patient and dentist is of great help to make a good oral impression. However, patients are frequently confused when information is given through traditional verbal description. The present study compared the effectiveness of the multimedia information delivery ways with the traditional verbal manner on patients' understanding level in oral impression taking. METHODS: The recruited 191 participating patients were randomly assigned to the control group (the verbal group) and two intervention groups (the video group and the picture group) according to the information delivery manner. After intervention, the patients' understanding degree was measured by questionnaire and performance evaluation of behavior feedback on the provided information quantitatively. Also, patients' self-assessment of satisfaction was interviewed by telephone. All data was analyzed by SPSS 14.0 software, and p≤0.05 was set as significant difference in advance. RESULTS: One-Way ANOVA and Chi-square showed there were no statistically significant differences in the mean age, gender composition, and educational level among the three groups (P>0.05). In both questionnaire assessment and performance evaluation, One-Way ANOVA followed by LSD indicate that the video group gained a higher score than the verbal group or the picture group (P<0.05). The questionnaire score in the picture group is significantly higher than in the control group (P<0.05), but no significant difference was shown between these two groups in patients' performance evaluation (P>0.05). Higher percentage of satisfaction was reported by patients in the two multimedia groups than that in the control group. DISCUSSION: Oral impression taking is a consecutive process that requires action cooperation between dentists and patients simultaneously. This particularity makes it more suitable for multimedia delivery. The delivery of tailored information using multimedia in this study was favored by most patients and could improve the degree of patient understanding of the oral impression taking procedures.


Assuntos
Técnica de Moldagem Odontológica , Multimídia/estatística & dados numéricos , Educação de Pacientes como Assunto , Doenças Estomatognáticas/terapia , Adolescente , Adulto , Idoso , Implantes Dentários , Odontólogos , Feminino , Conhecimentos, Atitudes e Prática em Saúde , Humanos , Masculino , Pessoa de Meia-Idade , Projetos Piloto , Método Simples-Cego , Inquéritos e Questionários , Adulto Jovem
15.
Int J Nanomedicine ; 12: 839-854, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-28182119

RESUMO

Scaffold supplements such as nanoparticles, components of the extracellular matrix, or growth factors have been incorporated in conventional scaffold materials to produce smart scaffolds for tissue engineering of damaged hard tissues. Due to increasing concerns on the clinical side effects of using large doses of recombinant bone-morphogenetic protein-2 in bone surgery, it is desirable to develop an alternative nanoscale scaffold supplement that is not only osteoinductive, but is also multifunctional in that it can perform other significant bone regenerative roles apart from stimulation of osteogenic differentiation. Because both amorphous calcium phosphate (ACP) and silica are osteoinductive, a biodegradable, nonfunctionalized, expanded-pore mesoporous silica nanoparticle carrier was developed for loading, storage, and sustained release of a novel, biosilicification-inspired, polyamine-stabilized liquid precursor phase of ACP for collagen biomineralization and for release of orthosilicic acid, both of which are conducive to bone growth. Positively charged poly(allylamine)-stabilized ACP (PAH-ACP) could be effectively loaded and released from nonfunctionalized expanded-pore mesoporous silica nanoparticles (pMSN). The PAH-ACP released from loaded pMSN still retained its ability to infiltrate and mineralize collagen fibrils. Complete degradation of pMSN occurred following unloading of their PAH-ACP cargo. Because PAH-ACP loaded pMSN possesses relatively low cytotoxicity to human bone marrow-derived mesenchymal stem cells, these nanoparticles may be blended with any osteoconductive scaffold with macro- and microporosities as a versatile scaffold supplement to enhance bone regeneration.


Assuntos
Regeneração Óssea/efeitos dos fármacos , Fosfatos de Cálcio/farmacologia , Nanopartículas/química , Osteogênese/efeitos dos fármacos , Polímeros/química , Dióxido de Silício/química , Alilamina/química , Animais , Materiais Biocompatíveis/química , Bovinos , Diferenciação Celular/efeitos dos fármacos , Colágeno/química , Humanos , Nanopartículas/administração & dosagem , Ácido Silícico/análise , Engenharia Tecidual , Tecidos Suporte
16.
Dent Mater ; 32(10): 1235-1247, 2016 10.
Artigo em Inglês | MEDLINE | ID: mdl-27497745

RESUMO

OBJECTIVES: An experimental discoloration-free calcium aluminosilicate cement has been developed with the intention of maximizing the beneficial attributes of tricalcium silicate cements and calcium aluminate cements. The present study examined the effects of this experimental cement (Quick-Set2) on the mineralogenic characteristics of osteogenic lineage-committed human dental pulp stem cells (hDPSCs), by comparing the cellular responses with a commercially available tricalcium silicate cement (white mineral trioxide aggregate (ProRoot(®) MTA); WMTA). METHODS: The osteogenic potential of hDPSCs exposed to the cements was examined using qRT-PCR for osteogenic gene expressions, Western blot for osteogenic-related protein expressions, alkaline phosphatase enzyme activity, Alizarin red S staining, Fourier transform infrared spectroscopy and transmission electron microscopy of extracellular calcium deposits. RESULTS: Results of the six assays indicated that osteogenic differentiation of hDPSCs was significantly enhanced after exposure to the tricalcium silicate cement or the experimental calcium aluminosilicate cement, with the former demonstrating better mineralogenic stimulation capacity. SIGNIFICANCE: The better osteogenic stimulating effect of the tricalcium silicate cement on hDPSCs may be due to its relatively higher silicate content, or higher OH(-) and Ca(2+) release. Further investigations with the use of in vivo animal models are required to validate the potential augmenting osteogenic effects of the experimental discoloration-free calcium aluminosilicate cement.


Assuntos
Silicatos de Alumínio/farmacologia , Cimentos Dentários/farmacologia , Polpa Dentária/citologia , Osteogênese/efeitos dos fármacos , Animais , Compostos de Cálcio , Argila , Humanos , Silicatos , Células-Tronco
17.
Acta Biomater ; 31: 312-325, 2016 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-26678828

RESUMO

UNLABELLED: Bisphosphonate-related osteonecrosis of the jaw (BRONJ) is a serious skeletal complication associated with the long-term oral or intravenous use of nitrogen-containing bisphosphonates (N-BPs). Here, we investigated the effects of an ionic cocktail prepared from water-soluble microfibrous borate glass on neutralizing the inhibitory effects of two heterocyclic N-BPs, risedronate or zoledronic acid, on osteoclastogenesis, apoptosis of differentiated osteoclasts and osteoclast function. Cell growth and proliferation assays were first performed on RAW 264.7 cells to optimize the concentrations of the ionic cocktail and N-BPs to be used for static cell culture. The pre-osteoclasts were then stimulated with RANKL to differentiate into osteoclasts. The effects of the ionic cocktail and N-BPs on osteoclast differentiation, apoptosis and function were subsequently examined using 3 series of experiments conducted at the gene, protein, morphological and functional levels. After concentration optimization, the ionic cocktail was found to partially reverse N-BP-induced inhibition of osteoclastogenesis, stimulation of osteoclasts apoptosis and reduction of osteoclast resorptive activity. Ultrastructural examination of osteoclasts that had been exposed to either N-BP identified classical features of late apoptosis and secondary necrosis, while osteoclasts exposed simultaneously to the concentration-optimized ionic cocktail and N-BPs exhibited only signs of early apoptosis that were possibly reversible. Taken together, the results of the 4 series of experiments indicate that the ionic cocktail produced from dissolution of borate glass dressings has the potential to rescue the adverse effects of heterocyclic N-BPs on osteoclast differentiation and function. These results warrant further confirmation using dynamic cell culture and small animal BRONJ models. STATEMENT OF SIGNIFICANCE: Long-term oral and intravenous use of nitrogen-containing bisphosphonates (N-BPs) may result in bisphosphonate-related osteonecrosis of the jaw (BRONJ) due to the suppression of normal bone turnover. There is no effective treatment for such a complication to date. This work reported the use of an ionic cocktail derived from water-soluble microfibrous borate glass to revert heterocyclic N-BP-induced inhibition of osteoclastogenesis, stimulation of osteoclasts apoptosis and reduction of osteoclasts resorption in static cell culture condition. This ionic cocktail may have the potential to be further developed into a new adjunctive treatment for BRONJ.


Assuntos
Boratos/química , Difosfonatos/química , Vidro/química , Nitrogênio/química , Osteoclastos/citologia , Osteonecrose/prevenção & controle , Animais , Apoptose , Reabsorção Óssea , Diferenciação Celular , Difosfonatos/efeitos adversos , Imidazóis/efeitos adversos , Macrófagos/citologia , Camundongos , Microscopia Eletrônica de Transmissão , Osteoclastos/efeitos dos fármacos , Osteoclastos/metabolismo , Estresse Oxidativo , Ligante RANK/metabolismo , Células RAW 264.7 , Espécies Reativas de Oxigênio/metabolismo , Ácido Risedrônico/efeitos adversos , Água/química , Ácido Zoledrônico
18.
Dent Mater ; 31(12): 1510-22, 2015 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-26494267

RESUMO

OBJECTIVES: In pulpal revascularization, a protective material is placed coronal to the blood clot to prevent recontamination and to facilitate osteogenic differentiation of mesenchymal stem cells to produce new dental tissues. Although mineral trioxide aggregate (MTA) has been the material of choice for clot protection, it is easily displaced into the clot during condensation. The present study evaluated the effects of recently introduced calcium silicate cements (Biodentine and TheraCal LC) on the viability and osteogenic differentiation of human dental pulp stem cells (hDPSCs) by comparing with MTA Angelus. METHODS: Cell viability was assessed using XTT assay and flow cytometry. The osteogenic potential of hDPSCs exposed to calcium silicate cements was examined using qRT-PCR for osteogenic gene expressions, alkaline phosphatase enzyme activity, Alizarin red S staining and transmission electron microscopy of extracellular calcium deposits. Parametric statistical methods were employed for analyses of significant difference among groups, with α=0.05. RESULTS: The cytotoxic effects of Biodentine and TheraCal LC on hDPSCs were time- and concentration-dependent. Osteogenic differentiation of hDPSCs was enhanced after exposure to Biodentine that was depleted of its cytotoxic components. This effect was less readily observed in hDPSCs exposed to TheraCal LC, although both cements supported extracellular mineralization better than the positive control (zinc oxide-eugenol-based cement). SIGNIFICANCE: A favorable tissue response is anticipated to occur with the use of Biodentine as a blood clot-protecting material for pulpal revascularization. Further investigations with the use of in vivo animal models are required to validate the potential adverse biological effects of TheraCal LC on hDPSCs.


Assuntos
Compostos de Alumínio/química , Bismuto/química , Compostos de Cálcio/química , Cimentos Dentários/química , Polpa Dentária/irrigação sanguínea , Polpa Dentária/citologia , Osteogênese/efeitos dos fármacos , Óxidos/química , Materiais Restauradores do Canal Radicular/química , Silicatos/química , Células-Tronco/efeitos dos fármacos , Adolescente , Adulto , Compostos de Alumínio/toxicidade , Bismuto/toxicidade , Compostos de Cálcio/toxicidade , Sobrevivência Celular , Cimentos Dentários/toxicidade , Combinação de Medicamentos , Citometria de Fluxo , Humanos , Teste de Materiais , Óxidos/toxicidade , Materiais Restauradores do Canal Radicular/toxicidade , Silicatos/toxicidade , Cimento de Óxido de Zinco e Eugenol/química , Cimento de Óxido de Zinco e Eugenol/toxicidade
19.
Arch Oral Biol ; 60(10): 1510-6, 2015 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-26263540

RESUMO

OBJECTIVE: The aim of this study is to investigate the expression of pannexin3 (Panx3) in human odontoblast-like cells (hOBs) and its hemichannel function in mediating ATP release. METHODS: RT-PCR and immunofluorescence analysis were used to detect the expression of pannexins (Panxs) in human dental pulp tissue and cultured cells. To determine the role of Panx3 in ATP release, hOBs were infected with Panx3-overexpression lentivirus, Panx3-shRNA lentivirus or control lentivirus and then stimulated with cold buffer. Intracellular ATP was monitored using quinacrine, and then semi-quantitatively analyzed. In the meantime, the ATP release was quantitatively analyzed using the bioluminescence method when the cells were exposed to cold stimulus. RESULTS: Panx3 mRNA and protein were found in dental pulp tissue and cultured cells. Upon cold stimulus, intracellular ATP was released into the extracellular space. Overexpression of Panx3 accelerated ATP release, whereas inhibition of Panx3 suppressed this process. CONCLUSION: Panx3 hemichannel is expressed in human odontoblast-like cells and mediates ATP release into the extracellular space.


Assuntos
Trifosfato de Adenosina/metabolismo , Conexinas/biossíntese , Odontoblastos/metabolismo , Adolescente , Adulto , Diferenciação Celular/fisiologia , Linhagem Celular , Células Cultivadas , Temperatura Baixa , Conexinas/genética , Polpa Dentária/citologia , Polpa Dentária/metabolismo , Sensibilidade da Dentina/genética , Sensibilidade da Dentina/metabolismo , Técnicas de Silenciamento de Genes , Humanos , Lentivirus/genética , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , RNA Interferente Pequeno/administração & dosagem , RNA Interferente Pequeno/genética , Adulto Jovem
20.
J Dent ; 42(9): 1115-23, 2014 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-24727119

RESUMO

OBJECTIVES: To evaluate the effects of different application sequence of calcium-containing desensitising pastes on bonding effectiveness and tubule occlusion during etch-and-rinse (E&R) adhesive restoration. METHODS: Seventy molars were sectioned parallel to the occlusal plane, polished and randomly divided into seven groups (n=10). Group 1 was etched with 35% phosphoric acid for 15s. Groups 2-4 were treated with different calcium-containing desensitisers, including an arginine-calcium carbonate (Arg-CaCO3)-containing paste, a casein phosphopeptide-amorphous calcium phosphate (CPP-ACP)-containing paste and a calcium-sodium phosphosilicate (Novamin)-containing paste, respectively. Afterward, these groups were etched with 35% phosphoric acid for 15s. Groups 5-7 were initially etched and then treated with Arg-CaCO3-, CPP-ACP- and Novamin-containing desensitisers, respectively. In each group, samples were equally distributed into two subgroups (n=5) to bond with either a two-step E&R adhesive Adper SingleBond 2 (SB) or a three-step E&R adhesive Adper ScotchBond Multi-Purpose (SBMP). The microtensile bond strengths (MTBS) were tested and fracture modes were analyzed by stereomicroscopy and field-emission scanning electron microscopy (FESEM). Eight additional dentine disks were prepared to evaluate tubule occlusion prior to bonding using FESEM. RESULTS: The application sequence of calcium-containing desensitising pastes did not significantly affect MTBS irrespective of two-step SB (P>0.05) or three-step SBMP E&R adhesives (P>0.05). Effective dentinal tubule occlusion was observed in the mode of etching-desensitising. CONCLUSIONS: Applying calcium-containing desensitisers (particularly Arg-CaCO3- and Novamin-based) after etching during E&R adhesive restoration could achieve effective tubule occlusion without affecting the bonding strength.


Assuntos
Cálcio/administração & dosagem , Colagem Dentária , Dessensibilizantes Dentinários/administração & dosagem , Adesivos Dentinários/química , Dentina/efeitos dos fármacos , Condicionamento Ácido do Dente/métodos , Adesividade , Arginina/administração & dosagem , Arginina/química , Cálcio/química , Carbonato de Cálcio/administração & dosagem , Carbonato de Cálcio/química , Caseínas/administração & dosagem , Caseínas/química , Cimentos Dentários/química , Dentina/ultraestrutura , Dessensibilizantes Dentinários/química , Fluoretos/administração & dosagem , Fluoretos/química , Vidro/química , Humanos , Teste de Materiais , Microscopia Eletrônica de Varredura , Fosfatos/administração & dosagem , Fosfatos/química , Ácidos Fosfóricos/química , Distribuição Aleatória , Cimentos de Resina/química , Estresse Mecânico , Propriedades de Superfície , Resistência à Tração , Fatores de Tempo
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