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1.
Sci Total Environ ; 806(Pt 3): 151279, 2022 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-34717988

RESUMO

This paper seeks to account for differences in productivity of the industrial and non-industrial activities in the productivity analysis framework. The Luenberger productivity indicator is widely applied to analyze the productivity change, and can be decomposed as it follows the additive structure. But there have been few studies on sector operation performance and industrial structure involving both the industrial and non-industrial inputs, output and air pollutant emissions. Resorting on the China's province-level data on energy, output and air pollutants from 2006 to 2019, we find that the industrial SO2 emissions, energy consumption and NOX emissions are the major factors leading to sector operation inefficiency. By decomposing the operation performance indicator (OPI), we observe that contribution to productivity change by energy consumption, air pollutant emissions and output is higher than contribution by the non-industrial variables. Furthermore, technical progress offsets negative efficiency growth. In order to implement energy conservation, emissions reduction and industrial restructuring at the provincial level, China's government should take efforts to improve the efficiency of non-industrial energy consumption and support the development of cleaner industries.

2.
Exp Ther Med ; 23(1): 16, 2022 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-34815768

RESUMO

Numerous microRNAs (miRNAs/miRs) have been demonstrated to serve oncogenic or suppressive roles in glioma. Exploration of the underlying molecular mechanism of miRNAs in the development and progression of glioma is beneficial for the identification of novel therapeutic targets. In the present study, the function of miR-25 in glioma progression, as well as its underlying mechanism, were investigated. It was determined that miR-25 was significantly upregulated in glioma tissues and cell lines compared with normal brain tissues and cells, respectively. Furthermore, high expression levels of miR-25 were associated with an advanced clinical stage. The knockdown of miR-25 expression significantly reduced glioma cell proliferation, migration and invasion. Cell adhesion molecule 2 (CADM2) was identified as a direct target of miR-25 in glioma cells. Moreover, CADM2 expression level was significantly downregulated and inversely correlated with miR-25 expression level in glioma tissues, indicating that the expression of CADM2 was negatively regulated by miR-25. The inhibition of CADM2 expression counteracted the effects on glioma cell proliferation, migration and invasion caused by miR-25 downregulation. Furthermore, CADM2 knockdown considerably promoted the proliferation and migration of glioma cells. In summary, the present study demonstrated that miR-25 was significantly upregulated in glioma and that it promoted glioma cell proliferation, migration and invasion, at least partially, by directly targeting CADM2. These findings expanded the understanding of the molecular mechanism that underlies glioma progression.

3.
Front Psychol ; 12: 717110, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34777097

RESUMO

Listeners utilize the immediate contexts to efficiently normalize variable vocal streams into standard phonology units. However, researchers debated whether non-speech contexts can also serve as valid clues for speech normalization. Supporters of the two sides proposed a general-auditory hypothesis and a speech-specific hypothesis to explain the underlying mechanisms. A possible confounding factor of this inconsistency is the listeners' perceptual familiarity of the contexts, as the non-speech contexts were perceptually unfamiliar to listeners. In this study, we examined this confounding factor by recruiting a group of native Cantonese speakers with sufficient musical training experience and a control group with minimal musical training. Participants performed lexical tone judgment tasks in three contextual conditions, i.e., speech, non-speech, and music context conditions. Both groups were familiar with the speech context and not familiar with the non-speech context. The musician group was more familiar with the music context than the non-musician group. The results evidenced the lexical tone normalization process in speech context but not non-speech nor music contexts. More importantly, musicians did not outperform non-musicians on any contextual conditions even if the musicians were experienced at pitch perception, indicating that there is no noticeable transfer in pitch perception from the music domain to the linguistic domain for tonal language speakers. The findings showed that even high familiarity with a non-linguistic context cannot elicit an effective lexical tone normalization process, supporting the speech-specific basis of the perceptual normalization process.

4.
Front Oncol ; 11: 731543, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34616680

RESUMO

Purpose: To evaluate the efficacy and toxicity of the two IC (induction chemotherapy) regimens, TPF (taxanes, cisplatin, and 5-fluorouracil) and TP (taxanes and cisplatin) combined with concurrent chemoradiotherapy (CCRT) in locally advanced nasopharyngeal carcinoma (LA-NPC) patients. Methods: Ultimately, we enrolled 213 patients at stage III-IVA in this retrospective study. The prognosis of TPF and TP was compared by Kaplan-Meier and Cox proportional hazard regression. The toxicities were evaluated according to CTCAE v4.0 and RTOG criteria. Results: TPF was found to have a higher 5-year DMFS in stage IVA and N2-3 patients. The optimal value of pretreatment SII was 432.48. A further subgroup analysis revealed that patients in stage IVA combined with SII ≥432.48 showed superior OS (P=0.038) and DMFS (P=0.028) from TPF. Also, SII was proved to be a prognostic element for PFS (HR 2.801, P=0.018) and DMFS (HR 3.735, P=0.032) in multivariate analysis, and IC regimen (HR 2.182, P=0.049) for predicting DMFS. The rate of grade 3-4 leukopenia (P=0.038), neutropenia (P=0.021), radiation oral mucositis (P=0.048), diarrhea (P=0.036), and ear damage (P=0.046) were more common in TPF group. Conclusion: Our study revealed that TPF regimen showed a higher 5-year DMFS for stage IVA and N2-3 patients, while for stage III and N0-1, TP might be ample. In high-risk LA-NPC patients (stage IVA combined with pretreatment SII ≥432.48), TPF had a higher 5-year OS and DMFS, with more grade 3-4 toxicities, but most of them were endurable.

5.
Brain Lang ; 222: 105028, 2021 11.
Artigo em Inglês | MEDLINE | ID: mdl-34597904

RESUMO

To achieve perceptual constancy, listeners utilize contextual cues to normalize speech variabilities in speakers. The present study tested the time course of this cognitive process with an event-related potential (ERP) experiment. The first neurophysiological evidence of speech normalization is observed in P2 (130-250 ms), which is functionally related to phonetic and phonological processes. Furthermore, the normalization process was found to ease lexical retrieval, as indexed by smaller N400 (350-470 ms) after larger P2. A cross-language vowel perception task was carried out to further specify whether normalization was processed in the phonetic and/or phonological stage(s). It was found that both phonetic and phonological cues in the speech context contributed to vowel normalization. The results suggest that vowel normalization in the speech context can be observed in the P2 time window and largely overlaps with phonetic and phonological processes.


Assuntos
Percepção da Fala , Fala , Eletroencefalografia , Potenciais Evocados , Feminino , Humanos , Masculino , Fonética , Acústica da Fala
6.
Nanomaterials (Basel) ; 11(10)2021 Oct 17.
Artigo em Inglês | MEDLINE | ID: mdl-34685191

RESUMO

The coupling strength between two-dimensional (2D) materials and substrate plays a vital role on thermal transport properties of 2D materials. Here we systematically investigate the influence of vacuum thermal annealing on the temperature-dependence of in-plane Raman phonon modes in monolayer graphene supported on silicon dioxide substrate via Raman spectroscopy. Intriguingly, raising the thermal annealing temperature can significantly enlarge the temperature coefficient of supported monolayer graphene. The derived temperature coefficient of G band remains mostly unchanged with thermal annealing temperature below 473 K, while it increases from -0.030 cm-1/K to -0.0602 cm-1/K with thermal annealing temperature ranging from 473 K to 773 K, suggesting the great impact of thermal annealing on thermal transport in supported monolayer graphene. Such an impact might reveal the vital role of coupling strength on phonon scattering and on the thermal transport property of supported monolayer graphene. To further interpret the thermal annealing mechanism, the compressive stress in supported monolayer graphene, which is closely related to coupling strength and is studied through the temperature-dependent Raman spectra. It is found that the variation tendency for compressive stress induced by thermal annealing is the same as that for temperature coefficient, implying the intense connection between compressive stress and thermal transport. Actually, 773 K thermal annealing can result in 2.02 GPa compressive stress on supported monolayer graphene due to the lattice mismatch of graphene and substrate. This study proposes thermal annealing as a feasible path to modulate the thermal transport in supported graphene and to design future graphene-based devices.

7.
Front Neurosci ; 15: 648143, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34658751

RESUMO

Objective: This study investigates whether the dynamic functional connectivity (dFC) of the amygdala subregions is altered in children with attention-deficit/hyperactivity disorder (ADHD). Methods: The dFC of the amygdala subregions was systematically calculated using a sliding time window method, for 75 children with ADHD and 20 healthy control (HC) children. Results: Compared with the HC group, the right superficial amygdala exhibited significantly higher dFC with the right prefrontal cortex, the left precuneus, and the left post-central gyrus for children in the ADHD group. The dFC of the amygdala subregions showed a negative association with the cognitive functions of children in the ADHD group. Conclusion: Functional connectivity of the amygdala subregions is more unstable among children with ADHD. In demonstrating an association between the stability of functional connectivity of the amygdala and cognitive functions, this study may contribute by providing a new direction for investigating the internal mechanism of ADHD.

8.
J Speech Lang Hear Res ; : 1-18, 2021 Oct 27.
Artigo em Inglês | MEDLINE | ID: mdl-34705567

RESUMO

Purpose It has been reported that tone language-speaking children with autism demonstrate speech-specific lexical tone processing difficulty, although they have intact or even better-than-normal processing of nonspeech/melodic pitch analogues. In this early efficacy study, we evaluated the therapeutic potential of Auditory-Motor Mapping Training (AMMT) in facilitating speech and word output for Mandarin-speaking nonverbal and low-verbal children with autism, in comparison with a matched non-AMMT-based control treatment. Method Fifteen Mandarin-speaking nonverbal and low-verbal children with autism spectrum disorder participated and completed all the AMMT-based treatment sessions by intoning (singing) and tapping the target words delivered via an app, whereas another 15 participants received control treatment. Generalized linear mixed-effects models were created to evaluate speech production accuracy and word production intelligibility across different groups and conditions. Results Results showed that the AMMT-based treatment provided a more effective training approach in accelerating the rate of speech (especially lexical tone) and word learning in the trained items. More importantly, the enhanced training efficacy on lexical tone acquisition remained at 2 weeks after therapy and generalized to untrained tones that were not practiced. Furthermore, the low-verbal participants showed higher improvement compared to the nonverbal participants. Conclusions These data provide the first empirical evidence for adopting the AMMT-based training to facilitate speech and word learning in Mandarin-speaking nonverbal and low-verbal children with autism. This early efficacy study holds promise for improving lexical tone production in Mandarin-speaking children with autism but should be further replicated in larger scale randomized studies. Supplemental Material https://doi.org/10.23641/asha.16834627.

9.
J Genet Genomics ; 2021 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-34606992

RESUMO

The nuclear receptor DAX-1 (encoded by the NR0B1 gene) is presented in the hypothalamic tissues in humans and other vertebrates. Human patients with NR0B1 mutations often have hypothalamic-pituitary defects, but the involvement of NR0B1 in hypothalamic development and function is not well understood. Here, we report the disruption of the nr0b1 gene in zebrafish causes abnormal expression of gonadotropins, a reduction in fertilization rate, and an increase in post-fasting food intake, which is indicative of abnormal hypothalamic functions. We find that loss of nr0b1 increases the number of prodynorphin (pdyn)-expressing neurons but decreases the number of pro-opiomelanocortin (pomcb)-expressing neurons in the zebrafish hypothalamic arcuate region (ARC). Further examination reveals that the proliferation of progenitor cells is reduced in the hypothalamus of nr0b1 mutant embryos accompanying with the decreased expression of genes in the Notch signaling pathway. Additionally, the inhibition of Notch signaling in wild-type embryos increases the number of pdyn neurons, mimicking the nr0b1 mutant phenotype. In contrast, ectopic activation of Notch signaling in nr0b1 mutant embryos decreases the number of pdyn neurons. Taken together, our results suggest that nr0b1 regulates neural progenitor proliferation and maintenance to ensure normal hypothalamic neuron development.

10.
Front Oncol ; 11: 714433, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34707987

RESUMO

Background: In this study, we evaluated the prognostic value of the plasma levels of Epstein-Barr virus (EBV) DNA in patients with nasopharyngeal carcinoma (NPC) at different treatment stages. Methods: We retrospectively analyzed the Data of 206 patients with NPC. Pre-neoadjuvant chemotherapy (pre-NACT), post-NACT, post-radiotherapy, and post-treatment plasma EBV DNA levels were used to establish prognostic nomograms. The concordance index (C-index) and calibration curves were used to compare the prognostic accuracy of the nomograms. The results were confirmed in a validation cohort consisting of patients who were tested for EBV DNA levels at all four stages of treatment. The Kaplan-Meier method was used to calculate the progression-free survival (PFS) and overall survival (OS). Survival differences were calculated using the log-rank test. Results: EBV DNA-positive patients had worse 3-year PFS and 5-year OS than EBV DNA-negative patients; this was true for pre-NACT (PFS: 82.7% vs. 57.3%, P < 0.001; OS: 90.9% vs. 68.7%, P = 0.08) and post-NACT (PFS: 85.0% vs. 50.6%, P < 0.001; OS: 91.7% vs. 65.7%; P = 0.001) EBV DNA levels but not for post-radiotherapy (PFS: 72.2% vs. 60.9%, P = 0.192; OS: 73.1% vs. 77.2%, P = 0.472) or post-treatment (PFS: 77.3% vs. 59.2%, P = 0.063; OS: 77.5% vs. 79.7%, P = 0.644) levels. Nomograms combining pre-NACT and post-NACT EBV DNA levels had a superior prognostic ability than those of post-radiotherapy and post-treatment EBV DNA levels. Conclusion: Pre-NACT EBV DNA levels combined with post-NACT EBV DNA levels can more reliably predict survival outcomes in patients with NPC.

11.
J Cell Sci ; 134(21)2021 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-34622921

RESUMO

Cardiac looping and trabeculation are key processes during cardiac chamber maturation. However, the underlying mechanisms remain incompletely understood. Here, we report the isolation, cloning and characterization of the proprotein convertase furina from the cardiovascular mutant loft in zebrafish. loft is an ethylnitrosourea-induced mutant and has evident defects in the cardiac outflow tract, heart looping and trabeculation, the craniofacial region and pharyngeal arch arteries. Positional cloning revealed that furina mRNA was barely detectable in loft mutants, and loft failed to complement the TALEN-induced furina mutant pku338, confirming that furina is responsible for the loft mutant phenotypes. Mechanistic studies demonstrated that Notch reporter Tg(tp1:mCherry) signals were largely eliminated in mutant hearts, and overexpression of the Notch intracellular domain partially rescued the mutant phenotypes, probably due to the lack of Furina-mediated cleavage processing of Notch1b proteins, the only Notch receptor expressed in the heart. Together, our data suggest a potential post-translational modification of Notch1b proteins via the proprotein convertase Furina in the heart, and unveil the function of the Furina-Notch1b axis in cardiac looping and trabeculation in zebrafish, and possibly in other organisms.

12.
ACS Appl Mater Interfaces ; 13(41): 49153-49162, 2021 Oct 20.
Artigo em Inglês | MEDLINE | ID: mdl-34632760

RESUMO

2D semiconductors with atomically thin body thickness have attracted tremendous research interest for high-performance nanoelectronics and optoelectronics. Most of the 2D semiconductors grown by chemical vapor deposition (CVD) methods suffer from rather low carrier mobility, small single-crystal size, and instability under ambient conditions. Here, we develop an improved CVD method with controllable reverse-gas flow to realize the direct growth of quality Bi2O2Se 2D single crystals on a mica substrate. The applied reverse flow significantly suppresses the random nucleation and thus promotes the lateral size of 2D Bi2O2Se crystals up to ∼750 µm. The Bi2O2Se field-effect transistors display high-room-temperature electron mobility up to ∼1400 cm2·V-1·s-1 and a well-defined drain current saturation. The on/off ratio of the Bi2O2Se transistor is larger than 107, and the sub-threshold swing is about 90 mV·dec-1. The responsivity, response time, and detectivity of Bi2O2Se photodetectors approach up to 60 A·W-1, 5 ms, and 2.4 × 1010 Jones at room temperature, respectively. Our results demonstrate large-size and high-quality Bi2O2Se grown by reverse-flow CVD as a high-performance channel material for next-generation transistors and photodetectors.

13.
J Neurooncol ; 154(3): 285-299, 2021 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-34478013

RESUMO

PURPOSE: Aberrant expression of long noncoding RNAs plays a pivotal role in tumorigenesis. Recently, several studies have showed that the LINC00152 gene is upregulated in a variety of tumors and plays an oncogene role; however, its underlying molecular mechanisms in glioblastoma remain unclear. In this study, we prepare to investigate the biological role and underlying molecular mechanisms of LINC00152 in glioblastoma cells. METHODS: Bioinformatics analysis to identify LINC00152 expression, Cell Counting kit-8 assay and Colony formation assay were used to evaluate proliferation, Flow cytometric analysis was used to evaluate apoptosis, Cell Matrigel invasion assay and Wound healing assay was used to evaluate invasion, Western blot analysis to check protein expression level, Mouse xenograft models was used to check cell proliferation in vivo. RESULTS: In this study, we found that LINC00152 was upregulated in gliomas and its expression was significantly associated with high tumor aggressiveness and poor outcomes for glioma patients. Functionally, the knockdown of LINC00152 not only inhibited malignant behaviors of glioma, such as proliferation and invasion of glioma cells and induced apoptosis in vitro but also suppressed tumorigenesis in vivo. Mechanistically, results of the bioinformatics analysis and experimental studies confirmed that LINC00152 and RAB10 as the targets of miR-107, and LINC00152 might act as a sponge for miR-107 to regulate the expression of RAB10 in glioblastoma. Additionally, silencing miR-107 reversed the effects induced by LINC00152 knockdown on glioblastoma cells both in vitro and in vivo. CONCLUSION: Our data suggested that LINC00152 is a candidate prognostic marker of glioma, and that the LINC00152/MIR-107/RAB10 axis plays a pivotal role in regulation of the glioma malignancy, and therefore, targeting the axis might be an effective therapeutic strategy to treat glioma.

14.
ACS Appl Mater Interfaces ; 13(38): 46164-46170, 2021 Sep 29.
Artigo em Inglês | MEDLINE | ID: mdl-34533939

RESUMO

Two-dimensional semiconductors exhibit strong light emission under optical or electrical pumping due to quantum confinement and large exciton binding energies. The regulation of the light emission shows great application potential in next-generation optoelectronic devices. Herein, by the physical vapor deposition strategy, we synthesize monolayer hexagonal-shaped WS2, and its photoluminescence intensity mapping show three-fold symmetric patterns with alternating bright and dark regions. Regardless of the length of the edges, all domains with S-terminated edges show lower photoluminescence intensity, while all regions with W-terminated edges exhibit higher photoluminescence intensity. The photoluminescence segmentation mechanism is studied in detail by employing Raman spectroscopy, atomic force microscopy, high-resolution transmission electron microscopy, and Kelvin probe force microscopy, and it is found to originate from different strain distributions in the S-terminated region and the W-terminated region. The optical band gap determined by the photoluminescence in the dark region is ∼2 meV lower than that in the bright region, implying that more strain is stored in the S-terminated region than in the W-terminated region. The photoluminescence segmentation vanishes in transferred hexagonal-shaped WS2 from the initial substrate to a fresh silicon substrate, further confirming the physical mechanism. Our results provide guidance for tuning the optical properties of two-dimensional semiconductors by controllable strain engineering.

15.
J Speech Lang Hear Res ; 64(11): 4468-4484, 2021 11 08.
Artigo em Inglês | MEDLINE | ID: mdl-34570628

RESUMO

Purpose Previous studies have shown enhanced pitch and impaired time perception in individuals with autism spectrum disorders (ASD). However, it remains unclear whether such deviated patterns of auditory processing depending on acoustic dimensions would transfer to the higher level linguistic pitch and time processing. In this study, we compared the categorical perception (CP) of lexical tones and voice onset time (VOT) in Mandarin Chinese, which utilize pitch and time changes, respectively, to convey phonemic contrasts. Method The data were collected from 22 Mandarin-speaking adolescents with ASD and 20 age-matched neurotypical controls. In addition to the identification and discrimination tasks to test CP performance, all the participants were evaluated with their language ability and phonological working memory. Linear mixed-effects models were constructed to evaluate the identification and discrimination scores across different groups and conditions. Results The basic CP pattern of cross-boundary benefit when perceiving both native lexical tones and VOT was largely preserved in high-functioning adolescents with ASD. The degree of CP of lexical tones in ASD was similar to that in typical controls, whereas the degree of CP of VOT in ASD was greatly reduced. Furthermore, the degree of CP of lexical tones correlated with language ability and digit span in ASD participants. Conclusions These findings suggest that the unbalanced acoustic processing capacities for pitch and time can be generalized to the higher level linguistic processing in ASD. Furthermore, the higher degree of CP of lexical tones correlated with better language ability in Mandarin-speaking individuals with ASD.

16.
J Speech Lang Hear Res ; 64(10): 3794-3802, 2021 10 04.
Artigo em Inglês | MEDLINE | ID: mdl-34473569

RESUMO

Purpose This study investigated the effect of cognitive load (CL) on the categorical perception (CP) of Mandarin lexical tones to discuss the application of the generalized pulse-skipping hypothesis. This hypothesis assumes that listeners might miss/skip temporal pulses and lose essential speech information due to CL, which consequently affects both the temporal and spectral dimensions of speech perception. Should CL decrease listeners' pitch sensitivity and impair the distinction of tone categories, this study would support the generalized pulse-skipping hypothesis. Method Twenty-four native Mandarin-speaking listeners were recruited to complete a dual-task experiment where they were required to identify or discriminate tone stimuli while concurrently memorizing six Chinese characters or graphic symbols. A no-load condition without a memory recall task was also included as a baseline condition. The position of categorical boundary, identification slope, between- and within-category discrimination, and discrimination peakedness were compared across the three conditions to measure the impact of CL on tone perception. The recall accuracy of Chinese characters and graphic symbols was used to assess the difficulty of memory recall. Results Compared with the no-load condition, both load conditions showed a boundary shift to Tone 3, shallower identification slope, poorer between-category discrimination, and lower discrimination peakedness. Within-category discrimination was negatively affected by CL in the graphic symbol condition only, not in the Chinese character condition. Conclusions CL degraded listeners' sensitivity to subtle fundamental frequency changes and impaired CP of Mandarin lexical tones. This provides support for the generalized pulse-skipping hypothesis. Besides, the involvement of lexical information modulated the effect of CL.


Assuntos
Percepção da Altura Sonora , Percepção da Fala , Cognição , Humanos , Idioma , Fala
17.
Front Oncol ; 11: 690213, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34422645

RESUMO

Gliomas are characterized by high incidence, recurrence and mortality all of which are significant challenges to efficacious clinical treatment. The hypoxic microenvironment in the inner core and intermediate layer of the tumor mass of gliomas is a critical contributor to glioma pathogenesis. In this study, we identified an upregulated lncRNA, OR7E156P, in glioma was identified. The silencing of OR7E156P inhibited cell invasion and DNA synthesis in vitro and tumor growth in vivo. OR7E156P was intricately linked to the HIF1A pathway. Hypoxia could induce OR7E156P expression, whereas OR7E156P silencing decreased HIF1A protein levels under hypoxic conditions. Hypoxia promoted glioma cell invasion and DNA synthesis, and HUVEC tube formation, whereas OR7E156P silencing partially reversed the cellular effects of hypoxia. HIF1A overexpression promoted, whereas OR7E156P silencing inhibited tumor growth; the inhibitory effects of OR7E156P silencing on tumor growth were partially reversed by HIF1A overexpression. miR-143 directly targeted OR7E156P and HIF1A, respectively. miR-143 inhibition increased HIF1A protein levels, promoted glioma cell invasion and DNA synthesis. Moreover, they enhanced HUVEC tube formation, whereas OR7E156P silencing partially reversed the cellular effects of miR-143 inhibition. HIF1A targeted the promoter region of miR-143 and inhibited miR-143 expression. Altogether a regulatory axis consisting of OR7E156P, miR-143, and HIF1A, was identified which is deregulated in glioma, and the process of the OR7E156P/miR-143/HIF1A axis modulating glioma cell invasion through ZEB1 and HUVEC tube formation through VEGF was demonstrated.

18.
Sensors (Basel) ; 21(16)2021 Aug 17.
Artigo em Inglês | MEDLINE | ID: mdl-34450964

RESUMO

Currently, simultaneous localization and mapping (SLAM) is one of the main research topics in the robotics field. Visual-inertia SLAM, which consists of a camera and an inertial measurement unit (IMU), can significantly improve robustness and enable scale weak-visibility, whereas monocular visual SLAM is scale-invisible. For ground mobile robots, the introduction of a wheel speed sensor can solve the scale weak-visibility problem and improve robustness under abnormal conditions. In this paper, a multi-sensor fusion SLAM algorithm using monocular vision, inertia, and wheel speed measurements is proposed. The sensor measurements are combined in a tightly coupled manner, and a nonlinear optimization method is used to maximize the posterior probability to solve the optimal state estimation. Loop detection and back-end optimization are added to help reduce or even eliminate the cumulative error of the estimated poses, thus ensuring global consistency of the trajectory and map. The outstanding contribution of this paper is that the wheel odometer pre-integration algorithm, which combines the chassis speed and IMU angular speed, can avoid the repeated integration caused by linearization point changes during iterative optimization; state initialization based on the wheel odometer and IMU enables a quick and reliable calculation of the initial state values required by the state estimator in both stationary and moving states. Comparative experiments were conducted in room-scale scenes, building scale scenes, and visual loss scenarios. The results showed that the proposed algorithm is highly accurate-2.2 m of cumulative error after moving 812 m (0.28%, loopback optimization disabled)-robust, and has an effective localization capability even in the event of sensor loss, including visual loss. The accuracy and robustness of the proposed method are superior to those of monocular visual inertia SLAM and traditional wheel odometers.

19.
Curr Med Sci ; 41(4): 737-745, 2021 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-34403099

RESUMO

OBJECTIVE: To evaluate the long-term outcome and prognostic factors of patients with nasopharyngeal carcinoma (NPC) from low-endemic regions of China who received definitive intensity-modulated radiation therapy (IMRT). METHODS: The clinical data from 608 patients with newly-diagnosed non-metastatic NPC who have received initial treatment at our cancer center from January, 2008 to December, 2013 were retrospectively reviewed. All patients received definitive IMRT, and 87.7% received platinum-based chemotherapy. RESULTS: The median follow-up duration was 51 months (follow-up rate, 98.5%; range, 10-106 months) for the entire cohort. The 5-year overall survival rate was 79.7%. The 5-year local relapse-free survival rate, regional relapse-free survival rate, distant metastasis-free survival rate and progression-free survival rate were 92.4%, 93.3%, 79.2% and 74.3%, respectively. A total of 153 patients had experienced treatment failure, with distant metastasis as the primary cause in 77.1% (118/153). Patients with T4 or N3 diseases had a significantly poorer prognosis than other subcategories. Stage T4 and N3 were closely associated with distant metastasis, with the metastatic rate of 29.3% and 45.5%, respectively. CONCLUSION: IMRT provides patients with non-metastatic NPC with satisfactory long-term survival. Both T stage and N stage are important prognostic factors for NPC patients. Patients with T4 or N3 diseases have significantly increased distant metastatic rates and poor survival time.

20.
Cell Rep ; 36(3): 109413, 2021 Jul 20.
Artigo em Inglês | MEDLINE | ID: mdl-34289355

RESUMO

Metabolic regulation strategies have been developed to redirect metabolic fluxes to production pathways. However, it is difficult to screen out target genes that, when repressed, improve yield without affecting cell growth. Here, we report a strategy using a quorum-sensing system to control small RNA transcription, allowing cell-density-dependent repression of target genes. This strategy is shown with convenient operation, dynamic repression, and availability for simultaneous regulation of multiple genes. The parameters Ai, Am, and RA (3-oxohexanoyl-homoserine lactone [AHL] concentrations at which half of the maximum repression and the maximum repression were reached and value of the maximum repression when AHL was added manually, respectively) are defined and introduced to characterize repression curves, and the variant LuxRI58N is identified as the most suitable tuning factor for shake flask culture. Moreover, it is shown that dynamic overexpression of the Hfq chaperone is the key to combinatorial repression without disruptions on cell growth. To show a broad applicability, the production titers of pinene, pentalenene, and psilocybin are improved by 365.3%, 79.5%, and 302.9%, respectively, by applying combinatorial dynamic repression.

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