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1.
Artigo em Chinês | MEDLINE | ID: mdl-33794635

RESUMO

Objective:To explore the predictive factors for asymptomatic airway hyperresponsiveness(AAHR) in patients with chronic rhinosinusitis with nasal polyps(CRSwNP). Methods:A total of 76 CRSwNP patients who were hospitalized in the Department of Otorhinolaryngology of Fenyang Hospital affiliated to Shanxi Medical University from May 2016 to October 2020 were retrospectively analyzed, including 40 patients in AAHR group and 36 patients in non-airway hyperresponsiveness(non-airway hyperresponsiveness, NAHR) group. The clinical symptoms, CT score of paranasal sinuses, eosinophil(EOS) count in peripheral blood and nasal polyp tissue were compared and analyzed. Logistic regression was used to analyze the risk factors of AAHR, and receiver operating characteristics curve was used to judge the predictive value of the parameters. Results:Compared with NAHR group, AAHR group had more symptoms of mouth breathing and postnasal drip, higher total score of CT in ethmoid sinus, sphenoid sinus, olfactory cleft, and more EOS count in peripheral blood and nasal polyp tissue. The differences were statistically significant. There was a positive correlation between EOS count in peripheral blood and in nasal polyp tissue(r=0.324, P<0.01). Postnasal drip, high posterior ethmoid sinus(PE) score and nasal polyp tissue EOS count were risk factors for AAHR. The predictive value of nasal polyp tissue EOS count was higher than that of PE score(AUC=0.786 and 0.685, respectively). When the PE score was ≥1.5, the sensitivity was 80.0% and the specificity was 55.6%. When the nasal polyp tissue EOS count was ≥5.67/HPF, the sensitivity was 82.5% and the specificity was 66.7%. Conclusion:The occurrence of AAHR in patients with CRSwNP was related to clinical symptoms, paranasal sinus CT score, peripheral and nasal polyp tissue EOS count. PE score and nasal polyp tissue EOS count can be used to predict AAHR, however nasal polyp tissue EOS has higher predictive value.


Assuntos
Pólipos Nasais , Seios Paranasais , Rinite , Sinusite , Doença Crônica , Eosinófilos , Humanos , Pólipos Nasais/complicações , Estudos Retrospectivos , Rinite/complicações , Sinusite/complicações
2.
Biomed Res Int ; 2021: 6626094, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33816621

RESUMO

Background: Pulmonary arterial hypertension (PAH) is a disease or pathophysiological syndrome which has a low survival rate with abnormally elevated pulmonary artery pressure caused by known or unknown reasons. In addition, the pathogenesis of PAH is not fully understood. Therefore, it has become an urgent matter to search for clinical molecular markers of PAH, study the pathogenesis of PAH, and contribute to the development of new science-based PAH diagnosis and targeted treatment methods. Methods: In this study, the Gene Expression Omnibus (GEO) database was used to downloaded a microarray dataset about PAH, and the differentially expressed genes (DEGs) between PAH and normal control were screened out. Moreover, we performed the functional enrichment analyses and protein-protein interaction (PPI) network analyses of the DEGs. In addition, the prediction of miRNA and transcriptional factor (TF) of hub genes and construction miRNA-TF-hub gene network were performed. Besides, the ROC curve was used to evaluate the diagnostic value of hub genes. Finally, the potential drug targets for the 5 identified hub genes were screened out. Results: 69 DEGs were identified between PAH samples and normal samples. GO and KEGG pathway analyses revealed that these DEGs were mostly enriched in the inflammatory response and cytokine-cytokine receptor interaction, respectively. The miRNA-hub genes network was conducted subsequently with 131 miRNAs, 7 TFs, and 5 hub genes (CCL5, CXCL12, VCAM1, CXCR1, and SPP1) which screened out via constructing the PPI network. 17 drugs interacted with 5 hub genes were identified. Conclusions: Through bioinformatic analysis of microarray data sets, 5 hub genes (CCL5, CXCL12, VCAM1, CXCR1, and SPP1) were identified from DEGs between control samples and PAH samples. Studies showed that the five hub genes might play an important role in the development of PAH. These 5 hub genes might be potential biomarkers for diagnosis or targets for the treatment of PAH. In addition, our work also indicated that paying more attention on studies based on these 5 hub genes might help to understand the molecular mechanism of the development of PAH.

3.
Int J Clin Pract ; : e14213, 2021 Apr 05.
Artigo em Inglês | MEDLINE | ID: mdl-33819377

RESUMO

OBJECTIVES: Type 2 diabetes mellitus(T2DM) can accelerate the clinical process of atherosclerosis(AS). Dipeptidyl peptidase-4 inhibitors(DPP-4Is) have potential anti-AS effects. And, we completed a meta-analysis of the changes in carotid intima-media thickness(CIMT), flow-mediated dilation(FMD), and pulse wave velocity(PWV) of DPP-4Is to research the effect of DPP-4Is in the progression of AS in T2DM patients. MATERIALS AND METHODS: We included RCTs that evaluated the impact of DPP-4Is on CIMT, FMD, and PWV compared to other treatments from PubMed, Cochrane trials, and Embase database before October 31, 2020. We selected the random-effect model and calculated the weighted mean difference(WMD) to evaluate the effect of CIMT, FMD, and PWV in T2DM patients. RESULTS: Through the meta-analysis, we found that DPP-4Is can significantly reduce CIMT in T2DM patients(WMD =-0.036, 95% CI:-0.055 to-0.017; p≤0.001). Based on the subgroup analysis, we found that CIMT was significantly decreased in patients with greater than 12 months of intervention and without cardiovascular diseases. Besides, we also found that DPP-4Is had a not significant efficacy on the improvement of FMD in T2DM patients(WMD=0.635, 95% CI: -0.112 to 1.383, p= 0.097). Our subgroup analysis showed that for T2DM patients who have cardiovascular diseases, DPP-4Is can significantly increase their FMD. In addition, we also found that DPP-4Is had an insignificant influence on PWV in T2DM patients(WMD= 0.424, 95% CI: -0.198 to 1.046, p= 0.18). but SGLT2 inhibitors may reduce the PWV of T2DM patients. CONCLUSIONS: DPP-4Is can alleviate the development of AS in T2DM patients to a certain extent by reducing CIMT. And, we believe that long-term use of DPP-4Is may be more helpful to alleviate the atherosclerotic development of T2DM without obvious cardiovascular history.

4.
Dalton Trans ; 50(11): 3959-3965, 2021 Mar 21.
Artigo em Inglês | MEDLINE | ID: mdl-33646238

RESUMO

A simple and efficient synthetic route to [RuCl2(NHC)(p-cymene)] and [Ru(CO3)(NHC)(p-cymene)] complexes making use of a weak base, under aerobic conditions, is reported. This method enables access to a series of NHC-ruthenium compounds with moderate to good yields under mild conditions. The Ru pre-catalysts were successfully used in olefin oxidation catalysis at low catalyst loading and reach complete conversion in short times.

5.
Huan Jing Ke Xue ; 42(4): 1724-1738, 2021 Apr 08.
Artigo em Chinês | MEDLINE | ID: mdl-33742808

RESUMO

Investigating the pollution conditions of trace elements in the Poyang Lake basin, identifying the pollution sources, evaluating the ecological risks play an important part in formulating the effective strategies for protecting the basin ecosystem. To understand the concentration characteristics, pollution levels, and ecological risks of twelve trace elements (Cd, Hg, Pb, As, Cu, Zn, Cr, Ni, Mn, Sb, W, and Sn) in the floodplain sediments of the five main tributaries in Poyang Lake basin in the past twenty years, we used the enrichment factor (EF) and modified pollution index (MPI) to quantify the pollution levels of the trace elements, and analyzed and discussed the pollution sources in the areas with significant pollution level changes. The results indicated that the concentrations of cadmium, zinc, manganese, tungsten and lead in the floodplain sediments of the Poyang Lake basin during the past twenty years have increased by 134%, 26%, 41%, 25%, and 8%, respectively, and mercury, arsenic, chromium, nickel, antimony, and tin have decreased by 35%, 15%, 22%, 10%, 14%, and 13%, respectively, while copper has not changed significantly. The concentrations of cadmium in the Raohe River, Ganjiang River, and Xinjiang River basins have increased by 331%, 151%, and 107%, while mercury decreased in the Fuhe River, Ganjiang River, and Xiushui River basins by 87%, 41%, and 40%, respectively. The classification results based on the EF indicated that the changes in the pollution levels of trace elements in the Poyang Lake basin were characterized by the aggravation of cadmium pollution and the degradation of mercury pollution, the pollution level of cadmium in the Ganjiang River basin was upgraded to moderate and significant, the pollution level of cadmium in the Le'an River was upgraded to very high, and the pollution level of mercury in the Fuhe River basin was reduced to unpolluted or minimal. The MPI classification results established that the increase in the cadmium pollution had intensified the comprehensive pollution in the Poyang Lake basin, causing the proportion of severely and heavily polluted sites to be raised from 17% to 33%. The MRI classification results also demonstrated that the proportion of very high ecological risk sites have increased from 11% to 22%. The element with the highest MRI contribution rate changed from mercury to cadmium, which was caused by the reduction of the ecological risk level from very high to moderate. The results demonstrated that the significant pollution changes in the trace elements in the floodplain sediments of the Poyang Lake basin were primarily caused by human activities, such as mineral exploitation, smelting, and industrial and agricultural production. Our findings can provide scientific evidence for the prevention and control of river pollution and the optimal management of a watershed ecosystem.

6.
Huan Jing Ke Xue ; 42(4): 1989-2002, 2021 Apr 08.
Artigo em Chinês | MEDLINE | ID: mdl-33742834

RESUMO

In order to evaluate the land quality geochemical survey achievement in the service of the accurate management of urban land resources, the initial area of the Xiong'an New District as urbanization pathfinder in China is chosen as the research subject. The sample points were set by differential classification, and the spatial interpolation accuracy of the soil elements at a plot scale and a quantitative assessment of the consistency of the land plot (pattern spot) prediction evaluation were studied under the conditions of different sampling densities. The regional geochemical variation values randomly distributed on the plane can be reflected quantitatively by differential classification sampling, which can meet the basic demand of the quality attribute of a single plot (map spot) by the accurate management of urban land resources. The spatial variability of soil elements is mostly middle to moderate, and Cd, Cu, Pb, Hg, Se, N, P, and other elements of high spatial variability are affected by human industrial and agricultural production activities. Under the same sampling density, the larger the element variation coefficient, the worse the spatial interpolation accuracy. Although the interpolation accuracy of the same element index is affected by the sampling density, the increase in the sampling density could not identify the continuous component on the structure of the soil element content. The soil environment is clean, and the heavy metal content is lower than the GB15618-2018 standard. The interpolation results are basically consistent with the grading results of the measured values, while the contents of N, P, and K of the nutrient indices vary greatly, and the predicted and measured geochemical grades of the plots (map spot) differ substantially under the influence of factors such as human disturbance and spatial variability. The quantitative evaluation of the six different sampling densities indicates that the 16 points·km-2 sampling density adopted in the geochemical survey and evaluation of urban land quality can satisfy the needs of an accurate control of urban land resources in the study area and similar areas. The research can provide key technologies to support and serve the accurate management of urban land resources for geochemical surveys and the evaluation of land quality in land parcel scale cities.

7.
Huan Jing Ke Xue ; 42(3): 1514-1522, 2021 Mar 08.
Artigo em Chinês | MEDLINE | ID: mdl-33742949

RESUMO

To understand the main factors influencing the translocation and accumulation of cadmium (Cd) in soil-crop systems in typical karst areas, 68 sets of paddy soil and rice grain samples were collected in Guangxi Province. These were used to analyze Cd concentrations and soil properties (pH, organic matter (OM) content, oxide content, and texture). Spearman's correlation coefficients and principal component analysis (PCA) were used to examine the effects of soil properties on Cd concentrations and identify the main influencing factors. The studied soils were highly enriched in iron oxide (TFe2O3), aluminum oxide (Al2O3), and manganese oxide (MnO) compared to background levels, with average concentrations of 20.2%, 19.0%, and 0.2%, respectively. However, the soils are relatively depleted in silica (SiO2), with an average concentration of 41.0%. The soils are strongly weathered and leached in study area, giving rise to rich occurrences of Fe-Mn nodules. The concentrations of TFe2O3 and MnO in the study soils were significantly correlated with soil Cd, rice seed Cd, and the Cd bioconcentration factor (BCF). The PCA analysis further showed that TFe2O3 and MnO in soils were the main factors affecting the migration and enrichment of Cd while soil pH, OM, and Al2O3 had less of an influence. Furthermore, SiO2 and soil texture indirectly affected the migration and enrichment of Cd. It is suggested that the Fe-Mn nodules effectively adsorb and immobilize Cd in the study area soils, acting as a heavy metal scavenger that reduced the biological accessibility of Cd.

8.
BMC Pulm Med ; 21(1): 88, 2021 Mar 16.
Artigo em Inglês | MEDLINE | ID: mdl-33726733

RESUMO

BACKGROUND: Patients with interstitial lung disease (ILD) are occasionally positive for anti-neutrophil cytoplasmic antibodies (ANCAs). Differences between ILDs secondary to microscopic polyangiitis (MPA) and isolated ANCA-positive idiopathic interstitial pneumonia (IIP) remain unclear. The aim of this study was to explore the differences in clinical features and outcomes between MPA-associated ILDs and isolated ANCA-positive IIPs. METHODS: We reviewed 1338 ILDs patients with available ANCA results and retrospectively analysed 80 patients who were ANCA-positive. MPA-associated ILDs (MPA-ILDs group) and isolated ANCA-positive IIPs (ANCA-IIPs group) were compared. RESULTS: Among 80 patients with ANCA-positive ILDs, 31 (38.75%) had MPA-ILDs, and 49 (61.25%) had isolated ANCA-positive IIPs. Compared with ANCA-IIPs group, patients in MPA-ILDs group had a higher proportion of fever (p = 0.006) and higher neutrophil count (p = 0.011), erythrocyte sedimentation rate (ESR) (p < 0.001) and C-reactive protein (CRP) (p = 0.005). Multivariable analysis showed that ESR level was an independent risk factor for mortality in all 80 ANCA-positive ILDs patients (HR 1.028, p = 0.001). Survival in MPA-ILDs group was lower than that in ANCA-IIPs group, and further stratified analysis revealed that ANCA-IIPs patients with elevated ESR or CRP had a worse prognosis than those with normal inflammation markers, with 5-year cumulative survival rates of 60.00%, 86.90% and 100.00% in MPA-ILDs and ANCA-IIPs with and without elevated inflammation markers, respectively. CONCLUSIONS: Among patients with ANCA-positive ILDs, the prognoses of ANCA-IIPs with normal inflammation markers, ANCA-IIPs with elevated inflammation markers and MPA-ILDs were sequentially poorer. Therefore, stratified treatment should be considered in the management of ILDs patients positive for ANCAs.

9.
Clin Infect Dis ; 72(3): 538-539, 2021 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-33527121
10.
Medicine (Baltimore) ; 100(3): e23947, 2021 Jan 22.
Artigo em Inglês | MEDLINE | ID: mdl-33545971

RESUMO

BACKGROUND: Subclinical hypothyroidism (SCH) can increase the risk of heart failure (HF) clinically. However, thyroxine therapy for patients with HF and SCH has the risk of developing tachyarrhythmias. At present, there is no sufficient evidence-based medical evidence for levothyroxine in the therapy of this situation, and the treatment issue is still controversial. Therefore, our meta-analysis aims to assess the effectiveness and safety of thyroxine therapy for patients with HF and SCH. METHODS: We searched the related randomized controlled trials that have been published in the following 7 electronic databases: PubMed, Cochrane Library, EMBASE, Chongqing VIP, China National Knowledge Infrastructure, Chinese biomedical literature database, and Wan Fang database. The treatment group was treated with routine HF therapy plus thyroxine, while the control group was treated with HF routine therapy. Main outcome measures effective rate and New York Heart Association classification; Secondary outcome measures included: left ventricular ejection fraction, quality of life score, brain natriuretic peptide / N-terminal pro brain natriuretic peptide, 6-minute walk test, and adverse events. After screening studies and extracting data, we will use Cochrane collaborative tools to evaluate the risk of bias to assess the methodological quality of the included randomized controlled trials. We will use STATA 14.0 software for data synthesis and statistical analysis. Both subgroup analysis and sensitivity analysis will be used to detect potential sources of heterogeneity. In addition, we will use sensitivity analysis to test the stability of the outcomes. If possible, we will perform a funnel chart and Eggers test evaluate publication bias. The quality of the evidence will be evaluated through the grades of recommendations assessment, development, and evaluation system. RESULTS: Our findings will be published in peer-reviewed journals. CONCLUSION: This research will provide evidence about the efficacy and safety of thyroxine in the treatment of patients with HF and SCH. Objective to provide evidence-based medicine basis for thyroxine treatment of patients with SCH and HF. REGISTRATION NUMBER: INPLASY2020100062.


Assuntos
Protocolos Clínicos , Insuficiência Cardíaca/tratamento farmacológico , Hipotireoidismo/tratamento farmacológico , Tiroxina/uso terapêutico , Insuficiência Cardíaca/fisiopatologia , Humanos , Hipotireoidismo/fisiopatologia , Metanálise como Assunto , Revisões Sistemáticas como Assunto
11.
Theranostics ; 11(1): 257-267, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33391473

RESUMO

Rationale: Platinum-based chemotherapy is one of treatment mainstay for patients with advanced lung squamous cell carcinoma (LUSC) but it is still a "one-size fits all" approach. Here, we aimed to investigate the predictive and monitoring role of circulating cell-free DNA (cfDNA) profiling for the outcome of first-line chemotherapy in patients with advanced LUSC. Methods: Peripheral blood samples of 155 patients from a phase IV trial and 42 cases from an external real-world cohort were prospectively collected. We generated a copy number variations-based classifier via machine learning algorithm to integrate molecular profiling of cfDNA, named RESPONSE SCORE (RS) to predict the treatment outcome. To monitor the treatment efficacy, cfDNA samples collected at different time points were subjected to an ultra-deep sequencing platform. Results: The results showed that patients with high RS showed substantially higher objective response rate than those with low RS in training set (P < 0.001), validation set (P < 0.001) and real-world cohort (P = 0.019). Furthermore, a significant difference was observed in both progression-free survival (training set, P < 0.001; validation set: P < 0.001; real-world cohort: P = 0.019) and overall survival (training set, P < 0.001; validation set: P = 0.037) between high and low RS group. Notably, variant allele frequency (VAF) calculated from an ultra-deep sequencing platform significantly reduced in patients experienced a complete or partial response after 2 cycles of chemotherapy (P < 0.001), while it significantly increased in these of non-responder (P < 0.001). Moreover, VAF undetectable after 2 cycles of chemotherapy was correlated with markedly better objective response rate (P < 0.001) and progression-free survival (P < 0.001) than those with detectable VAF. Conclusions: These findings indicated that the RS, a circulating cfDNA sequencing-based stratification index, could help to guide first-line chemotherapy in advanced LUSC. The change of VAF is valuable to monitor the treatment response.

12.
Int J Mol Sci ; 22(2)2021 Jan 11.
Artigo em Inglês | MEDLINE | ID: mdl-33440839

RESUMO

Survival motor neuron (SMN) is ubiquitously expressed in many cell types and its encoding gene, survival motor neuron 1 gene (SMN1), is highly conserved in various species. SMN is involved in the assembly of RNA spliceosomes, which are important for pre-mRNA splicing. A severe neurogenic disease, spinal muscular atrophy (SMA), is caused by the loss or mutation of SMN1 that specifically occurred in humans. We previously reported that SMN plays roles in stem cell biology in addition to its roles in neuron development. In this study, we investigated whether SMN can improve the propagation of spermatogonia stem cells (SSCs) and facilitate the spermatogenesis process. In in vitro culture, SSCs obtained from SMA model mice showed decreased growth rate accompanied by significantly reduced expression of spermatogonia marker promyelocytic leukemia zinc finger (PLZF) compared to those from heterozygous and wild-type littermates; whereas SMN overexpressed SSCs showed enhanced cell proliferation and improved potency. In vivo, the superior ability of homing and complete performance in differentiating progeny was shown in SMN overexpressed SSCs in host seminiferous tubule of transplant experiments compared to control groups. To gain insights into the roles of SMN in clinical infertility, we derived human induced pluripotent stem cells (hiPSCs) from azoospermia patients (AZ-hiPSCs) and from healthy control (ct-hiPSCs). Despite the otherwise comparable levels of hallmark iPCS markers, lower expression level of SMN1 was found in AZ-hiPSCs compared with control hiPSCs during in vitro primordial germ cell like cells (PGCLCs) differentiation. On the other hand, overexpressing hSMN1 in AZ-hiPSCs led to increased level of pluripotent markers such as OCT4 and KLF4 during PGCLC differentiation. Our work reveal novel roles of SMN in mammalian spermatogenesis and suggest new therapeutic targets for azoospermia treatment.


Assuntos
Diferenciação Celular , Células Germinativas/citologia , Células Germinativas/metabolismo , Proteína 1 de Sobrevivência do Neurônio Motor/genética , Animais , Azoospermia/etiologia , Azoospermia/metabolismo , Autorrenovação Celular , Sobrevivência Celular/genética , Células Cultivadas , Modelos Animais de Doenças , Expressão Gênica , Humanos , Células-Tronco Pluripotentes Induzidas/citologia , Células-Tronco Pluripotentes Induzidas/metabolismo , Masculino , Camundongos , Neurônios Motores/metabolismo , Espermatogônias/citologia , Espermatogônias/metabolismo , Proteína 1 de Sobrevivência do Neurônio Motor/metabolismo
13.
Inflammation ; 2021 Jan 07.
Artigo em Inglês | MEDLINE | ID: mdl-33415536

RESUMO

Chronic obstructive pulmonary disease (COPD)/pulmonary emphysema is driven by the dysregulated airway inflammation and primarily influenced by the interaction between cigarette smoking (CS) and the individual's susceptibility. The inflammation in COPD involves both innate and adaptive immunity. By binding to its specific ligands, chemokine receptor CXCR3 plays an important role in regulating tissue inflammation and damage. In acute animal model challenged with either CS or pathogens, CXCR3 knockout (KO) attenuated lung inflammation and pathology. However, the role of CXCR3 in CS-induced chronic airway inflammation and pulmonary emphysema remains unknown. In this present study, we investigated the effect of CXCR3 in CS-induced pulmonary emphysema in an animal model, and the association between CXCR3 single nucleotide polymorphisms (SNPs) and COPD susceptibility in human subjects. We found that after chronic exposure to side stream CS (SSCS) for 24 weeks, CXCR3 KO mice demonstrated significant airspace enlargement expressed by mean linear intercept (Lm) compared with the wild-type (WT) mice. Consistently, CXCR3 KO mice had significantly higher BAL fluid macrophages and neutrophils, TNFα, and lung homogenate MMP-9 and MMP-12. Through genetic analysis of CXCR3 polymorphisms in a cohort of COPD patients with Han Chinese ethnicity, one CXCR3 SNP, rs2280964, was found to be genetically related to COPD susceptibility. Furthermore, CXCR3 SNP rs2280964 was significantly associated with the levels of serum MMP-9 in COPD patients. Our data from both animal and human studies revealed a novel role of CXCR3 possibly via influencing MMP9 production in the pathogenesis and progression of CS-associated COPD/pulmonary emphysema.

14.
Science ; 371(6527): 405-410, 2021 01 22.
Artigo em Inglês | MEDLINE | ID: mdl-33479154

RESUMO

Infection triggers expansion and effector differentiation of T cells specific for microbial antigens in association with metabolic reprograming. We found that the glycolytic enzyme lactate dehydrogenase A (LDHA) is induced in CD8+ T effector cells through phosphoinositide 3-kinase (PI3K) signaling. In turn, ablation of LDHA inhibits PI3K-dependent phosphorylation of Akt and its transcription factor target Foxo1, causing defective antimicrobial immunity. LDHA deficiency cripples cellular redox control and diminishes adenosine triphosphate (ATP) production in effector T cells, resulting in attenuated PI3K signaling. Thus, nutrient metabolism and growth factor signaling are highly integrated processes, with glycolytic ATP serving as a rheostat to gauge PI3K-Akt-Foxo1 signaling in the control of T cell immunity. Such a bioenergetic mechanism for the regulation of signaling may explain the Warburg effect.


Assuntos
Linfócitos T CD8-Positivos/imunologia , Glicólise , Lactato Desidrogenase 5/metabolismo , Fosfatidilinositol 3-Quinase/metabolismo , Animais , Linfócitos T CD8-Positivos/enzimologia , Proteína Forkhead Box O1/metabolismo , Humanos , Lactato Desidrogenase 5/genética , Listeria monocytogenes , Listeriose/enzimologia , Listeriose/imunologia , Camundongos , Camundongos Mutantes , Fosforilação , Proteínas Proto-Oncogênicas c-akt/metabolismo , Transdução de Sinais
15.
Arch Pharm Res ; 44(2): 194-204, 2021 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-33502677

RESUMO

Chemerin exhibits an inhibitory effect on hepatocellular carcinoma; however, the underlying mechanism is unclear. Here, low chemerin expression was confirmed in samples of liver cancer patients and hepatoma cells. Chemerin altered hepatoma cell morphology but had no effect on normal hepatocytes. Chemerin inhibited proliferation of several human hepatoma cell lines. Real-time PCR detection of hepatocellular carcinoma markers showed that mRNA levels of albumin and A-type gamma-glutamyl transferase increased whereas those of alpha-fetoprotein, alkaline phosphatase, B-type gamma-glutamyl transferase, insulin-like growth factor II, and human telomerase reverse transcriptase decreased in chemerin-treated SMMC7721 cells. Western blotting revealed that chemerin up-regulated albumin and vimentin expressions, and downregulated alpha-fetoprotein expression. Phosphorylated STAT3 was significantly up-regulated, whereas phosphorylated ERK and AKT were significantly downregulated by chemerin. Chemerin decreased phosphorylated ERK and AKT expression and the cell proliferation induced by PI3K activator 740 Y-P but could not significantly alter phosphorylated STAT3 expression and the cell growth induced by STAT3 inhibitor NSC74859. In conclusion, chemerin reversed the malignant phenotype and induced SMMC7721 cell differentiation by inhibiting MAPK/ERK and PI3K/AKT signaling; growth inhibition by chemerin is not directly related to the JAK/STAT signaling pathway. Our study provides novel evidence that chemerin could be utilized for liver cancer treatment.

16.
Artigo em Inglês | MEDLINE | ID: mdl-33421108

RESUMO

OBJECTIVE: To explore the associations of duration and quality of sleep during pregnancy with preterm birth and small for gestational age (SGA). METHODS: A prospective study was carried out on 1082 healthy women with singleton pregnancies from Chengdu, China. Self-report questionnaires, including duration and quality of sleep and other information, were administered at 8-12, 24-28, and 32-36 weeks of pregnancy. Data on gestational age and weight and length of the neonates were recorded after delivery. After controlling the potential confounders, a multivariable logistic regression model was performed to evaluate whether duration and quality of sleep were associated with preterm birth and SGA. RESULTS: Participants with short duration of sleep during the third trimester were more likely to report preterm birth (odds ratio [OR] 2.16, 95% confidence interval [CI] 1.26-4.81) and SGA (OR 2.67, 95% CI 1.18-6.54). Participants with poor quality of sleep during the third trimester were at high risk for preterm birth (OR 2.26, 95% CI 1.29-5.84) and SGA (OR 2.08, 95% CI 1.19-5.38). CONCLUSION: Short duration and poor quality of sleep during pregnancy are associated with an increased risk of preterm birth and SGA. Sleep characteristics should be assessed during prenatal evaluations to decrease adverse maternal and fetal outcomes.

17.
Huan Jing Ke Xue ; 42(1): 333-342, 2021 Jan 08.
Artigo em Chinês | MEDLINE | ID: mdl-33372485

RESUMO

The nationwide Se-enriched threshold plays a key role in identifying China's selenium-enriched land resources and developing characteristic agricultural practices. In this study, we used the cooperative data of 10222 sets of crops and roots in China for the past 10 years with a systematic analysis of the selenium content characteristics of the soil and the status of selenium-enriched agricultural products. The preliminary estimates of the selenium-enriched threshold based on a bulk crop-soil linear model and population selenium-intake are presented. Finally, a collaborative analysis model of soil selenium-enrichment rate and crop selenium-enrichment rate is established, coming up with the nationwide Se-enriched threshold:total selenium ≥ 0.40 µg·g-1 in paddy soil, and total selenium ≥ 0.30 µg·g-1 in dryland soil. The threshold passed the feasibility test in 13 provinces, providing strong support for the China Geological Survey to formulate and promulgate this technical standard for the delimitation of the natural selenium-enriched land.

18.
Mol Biol Evol ; 2020 Dec 07.
Artigo em Inglês | MEDLINE | ID: mdl-33283852

RESUMO

The rise and expansion of Tibetan Empire in the 7th to 9th centuries AD affected the course of history across East Eurasia, but the genetic impact of Tibetans on surrounding populations remains undefined. We sequenced 60 genomes for four populations from Pakistan and Tajikistan to explore their demographic history. We showed that the genomes of Balti people from Baltistan were comprised of 22.6% ∼ 26% Tibetan ancestry. We inferred a single admixture event and dated it to about 39 to 21 generations ago, a period that postdated the conquest of Baltistan by the ancient Tibetan Empire. The analyses of mitochondrial DNA, Y, and X chromosome data indicated that both ancient Tibetan males and females were involved in the male-biased dispersal. Given the fact that the Balti people adopted Tibetan language and culture in history, our study suggested the impact of Tibetan Empire on Baltistan involved dominant cultural and minor demic diffusion.

19.
Entropy (Basel) ; 22(9)2020 Aug 31.
Artigo em Inglês | MEDLINE | ID: mdl-33286731

RESUMO

Emotional and physical stress can cause various health problems. In this paper, we used tissue blood oxygen saturation (StO2), a newly proposed physiological signal, to classify the human stress. We firstly constructed a public StO2 database including 42 volunteers subjected to two types of stress. During the physical stress experiment, we observed that the facial StO2 right after the stress can be either increased or decreased comparing to the baseline. We investigated the StO2 feature combinations for the classification and found that the average StO2 values from left cheek, chin, and the middle of the eyebrow can provide the highest classification rate of 95.56%. Comparison with other stress classification method shows that StO2 based method can provide best classification performance with lowest feature dimension. These results suggest that facial StO2 can be used as a promising features to identify stress states, including emotional and physical stress.

20.
BMC Genomics ; 21(1): 857, 2020 Dec 02.
Artigo em Inglês | MEDLINE | ID: mdl-33267780

RESUMO

BACKGROUND: Ammonia is one of the most common toxicological environment factors affecting shrimp health. Although ammonia tolerance in shrimp is closely related to successful industrial production, few genetic studies of this trait are available. RESULTS: In this study, we constructed a high-density genetic map of the Pacific white shrimp (Litopenaeus vannamei) using specific length amplified fragment sequencing (SLAF-seq). The constructed genetic map contained 17,338 polymorphic markers spanning 44 linkage groups, with a total distance of 6360.12 centimorgans (cM) and an average distance of 0.37 cM. Using this genetic map, we identified a quantitative trait locus (QTL) that explained 7.41-8.46% of the phenotypic variance in L. vannamei survival time under acute ammonia stress. We then sequenced the transcriptomes of the most ammonia-tolerant and the most ammonia-sensitive individuals from each of four genetically distinct L. vannamei families. We found that 7546 genes were differentially expressed between the ammonia-tolerant and ammonia-sensitive individuals. Using QTL analysis and the transcriptomes, we identified one candidate gene (annotated as an ATP synthase g subunit) associated with ammonia tolerance. CONCLUSIONS: In this study, we constructed a high-density genetic map of L. vannamei and identified a QTL for ammonia tolerance. By combining QTL and transcriptome analyses, we identified a candidate gene associated with ammonia tolerance. Our work provides the basis for future genetic studies focused on molecular marker-assisted selective breeding.

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