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1.
Physiol Plant ; 167(4): 564-584, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-30561011

RESUMO

Abrupt drought-flood alternation (T1) is a meteorological disaster that frequently occurs during summer in southern China and the Yangtze river basin, often causing a significant loss of rice production. In this study, the response mechanism of yield decline under abrupt drought-flood alternation stress at the panicle differentiation stage was analyzed by looking at the metabolome, proteome as well as yield and physiological and biochemical indexes. The results showed that drought and flood stress caused a decrease in the yield of rice at the panicle differentiation stage, and abrupt drought-flood alternation stress created a synergistic effect for the reduction of yield. The main reason for the decrease of yield per plant under abrupt drought-flood alternation was the decrease of seed setting rate. Compared with CK0 (no drought and no flood), the net photosynthetic rate and soluble sugar content of T1 decreased significantly and its hydrogen peroxidase, superoxide dismutase, peroxidase activity increased significantly. The identified differential metabolites and differentially expressed proteins indicated that photosynthesis metabolism, energy metabolism pathway and reactive oxygen species response have changed strongly under abrupt drought-flood alteration stress, which are factors that leads to the rice grain yield reduction.

2.
J Genet ; 92(2): 281-7, 2013.
Artigo em Inglês | MEDLINE | ID: mdl-23970084

RESUMO

The study of 1000-grain weight (TGW) and percentage of grains with chalkiness (PGWC) is very important in rice. In this study, a set of introgression lines (ILs), derived from Sasanishiki/Habataki with Sasanishiki as the recurrent parent, were used to detect correlations and quantitative trait loci (QTL) on TGW and PGWC in two different environments. Phenotypic correlation analysis showed that there was no significant correlation between TGW and PGWC in both environments, which indicated that the linkage of TGW and PGWC traits could be broken via suitable population. A total of 20 QTL were detected in both environments, nine QTL for 1000-paddy-grain weight (PTGW), five QTL for 1000-brown-grain weight (BTGW) and six QTL for percentage of grains with chalkiness (PGWC). Moreover, five QTL, qPTGW3, qPTGW8.2, qPTGW11.1 for PTGW and qPGWC1.1, qPGWC1.2 for PGWC, were stably expressed in both environments. Phenotypic values were significantly different (P < 0.01) between the introgression lines carrying these five QTL alleles and the genetic background parent, Sasanishiki. The introgression lines carrying these QTL also represent a useful genetic resource in the context of rice yield and quality improvement via a design-breeding approach.


Assuntos
Mapeamento Cromossômico , Oryza/crescimento & desenvolvimento , Oryza/genética , Locos de Características Quantitativas , Cruzamentos Genéticos , Grão Comestível/genética , Grão Comestível/crescimento & desenvolvimento , Ligação Genética , Valor Nutritivo/genética , Fenótipo , Melhoria de Qualidade
3.
Ying Yong Sheng Tai Xue Bao ; 22(5): 1169-74, 2011 May.
Artigo em Chinês | MEDLINE | ID: mdl-21812290

RESUMO

By the method of water culture, the low-phosphorus tolerance of 221 lines of Xieqingzao B//Xieqingzao B/Dongxiang wild rice BC1 F9 populations was indentified. The morphological indices including plant height, leaf age, yellow leaf number, and shoot dry mass as well as the physiological indices including MDA, soluble sugar, and shoot phosphorus content were measured, also, the phosphorus efficiency was calculated, and the correlations among the indices were analyzed. All the 221 lines had differences in the seven test indices, and the low-phosphorus tolerance lines under low-phosphorus stress had higher values of relative leaf age, relative plant height, relative shoot dry mass, and relative soluble sugar content, but lower values of relative yellow leaf number and relative malondialdehyde content. The relative shoot phosphorus content had less difference. Phosphorus efficiency was positively correlated with phosphorus utilization efficiency and phosphorus uptake efficiency, and the correlation between phosphorus efficiency and phosphorus utilization efficiency was at significant level (P < 0.01), suggesting that the low-phosphorus tolerance capability of the low-phosphorus tolerance lines was mainly attributed to the high phosphorus utilization efficiency of the lines, namely, low-phosphorus tolerance lines had stronger capability in synthesizing dry mass with per unit phosphorus uptake.


Assuntos
Oryza/genética , Oryza/fisiologia , Fósforo/metabolismo , Adaptação Fisiológica , Cruzamentos Genéticos , Genótipo , Fósforo/análise
4.
Yao Xue Xue Bao ; 40(3): 225-30, 2005 Mar.
Artigo em Chinês | MEDLINE | ID: mdl-15952593

RESUMO

AIM: To explore the effect and the possible mechanism of ginsenoside Rb1 on beta-amyloid peptide (beta-AP)(25-35) -induced tau protein hyperphosphorylation in cortical neurons. METHODS: Western blotting and immunocytochemical staining were used to detect tau phosphorylation level, total tau and glycogen synthase kinase-3beta (GSK-3beta) in cortical neurons. RESULTS: After exposure to beta-AP(25-35) (20 micromol x L(-1)) for 12 h, the levels of tau protein phosphorylation in the sites of Ser 396, Ser 199/202, Thr 231 and total tau were raised. Meanwhile, the expression of GSK-3beta also increased. Pretreatment with ginsenoside Rbl or lithium chloride, a specific inhibitor of GSK-3beta, markedly reduced beta-AP(25-35)-induced tau hyperphosphorylation and the expression of GSK-3beta. CONCLUSION: Ginsenoside Rb1 can attenuate beta AP(25-35)-induced tau protein hyperphosphorylation in cortical neurons by inhibiting the expression of GSK-3beta.


Assuntos
Peptídeos beta-Amiloides/antagonistas & inibidores , Córtex Cerebral/metabolismo , Ginsenosídeos/farmacologia , Quinase 3 da Glicogênio Sintase/metabolismo , Fragmentos de Peptídeos/antagonistas & inibidores , Proteínas tau/metabolismo , Animais , Córtex Cerebral/citologia , Feminino , Feto , Ginsenosídeos/isolamento & purificação , Glicogênio Sintase Quinase 3 beta , Neurônios/metabolismo , Panax/química , Fosforilação , Plantas Medicinais/química , Ratos , Ratos Sprague-Dawley
5.
Sheng Li Xue Bao ; 57(2): 154-60, 2005 Apr 25.
Artigo em Chinês | MEDLINE | ID: mdl-15830099

RESUMO

The present study was aimed to investigate the effects of ginsenoside Rb1 on okadaic acid (OA)-induced Tau hyperphosphorylation in hippocampal neurons of Sparague-Dawley rat and to explore its possible mechanism. Animals were randomly divided into four groups. Group 1 received dimethysulphoxide (DMSO) injection (vehicle group), group 2 only received OA injection (OA group), group 3 was pretreated with Rb1 and then received OA injection (Rb1 pretreatment group), and the group 4 was an intact control group. The animals in group 3 were injected intraperitoneally with various doses of Rb1 at 5, 10, and 20 mg/kg (once a day for 14 d). On the thirteen day of pretreatment, animals in Rb1 pretreatment group as well as animals in OA group received a bolus injection of 0.483 microg of OA (1.5 microl of solution in DMSO) at right dorsal aspect of hippocampus to induce Tau hyperphosphrylation. The brains were harvested one day after the last treatment. In all groups, the morphology of neurofibrils, phosphorylation of Tau protein, and the activity of phosphatase 2A (PP2A) were investigated. In OA group, the Bielschowski's assay revealed darkened and uneven neurofibrils staining in the hippocampus. The immunohistochemistry results showed a significant increase in Thr(231) phosphorylation of Tau protein in OA group relative to the control group (P<0.01). OA injection also markedly decreased PP2A activity (P<0.01). Western blot confirmed Thr(231) phosphorylation of Tau protein and it also detected phosphorylation of Ser(396) of Tau protein. The animals with Rb1 pretreatment displayed even staining of neurofibrils and normal pattern of fiber organization. Rb1 pretreatment also attenuated Thr(231) and Ser(396) hyperphosphorylations of Tau protein, and restored PP2A activity compared to the OA group (P<0.01). These results indicate that OA-induced hyperphosphorylation of Tau protein in rat hippocampal neurons can be attenuated by the pretreatment of ginsenoside Rb1. These data also implicate that Rb1 has potential neuroprotective effects on Tau-related neuropathology.


Assuntos
Ginsenosídeos/farmacologia , Hipocampo/citologia , Neurônios/metabolismo , Proteínas tau/metabolismo , Doença de Alzheimer/metabolismo , Animais , Masculino , Neurônios/fisiologia , Fármacos Neuroprotetores/farmacologia , Ácido Okadáico , Fosforilação/efeitos dos fármacos , Distribuição Aleatória , Ratos , Ratos Sprague-Dawley
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