Your browser doesn't support javascript.
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 44
Filtrar
Mais filtros










Tipo de estudo
Intervalo de ano de publicação
1.
Photodiagnosis Photodyn Ther ; : 101596, 2019 Nov 05.
Artigo em Inglês | MEDLINE | ID: mdl-31704505

RESUMO

BACKGROUND: This study aimed to evaluate the effects of 5-aminolevulinic acid photodynamic therapy (ALA-PDT) on the improvement of symptoms and recurrence rate in patients with vulvar lichen sclerosus (VLS) and observe its side effects. METHODS: The symptom scores before and after photodynamic therapy (PDT) in 13 enrolled patients with VLS were analyzed retrospectively. All patients were followed-up for at least 6 months to evaluate the recurrence rate after PDT. The patients were treated with PDT only during the study period. During the PDT treatment, a 20% 5-aminolevulinic acid solution was applied to the lesions and marginal areas for 3 hours, and the entire area was then irradiated with 635 nm red light of 80 J/cm2 at 80 mW/cm2 for 30 minutes. RESULTS: In this study, the effective rate of PDT was 92.31%. Lesions recurred in two patients at 6 months after PDT. Post-treatment, the total subjective, total objective, and the Dermatological Life Quality Index scores changed from 11.4, 4.3, and 13.4 at baseline to 4.9, 2, and 5.9, respectively. The difference was statistically significant (p <0.05). PDT was mildly toxic in most patients. CONCLUSIONS: ALA-PDT is a safe and effective method for the treatment of VLS, and the therapeutic effects can be maintained for at least 3 months. The therapeutic effects may decrease during the 3-6-month period after PDT.

3.
Photodiagnosis Photodyn Ther ; 28: 114-119, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31479803

RESUMO

BACKGROUND: Aminolaevulinic acid-mediated photodynamic therapy (ALA-PDT) is used to treat condyloma acuminata (CA), yielding a high clearance rate and low recurrence rate. Consecutive human papillomavirus (HPV) DNA detection can be used to dynamically monitor the therapeutic efficiency of PDT. Here, we evaluated the efficacy of ALA-PDT in the context of different HPV infection states. METHODS: One hundred thirty-eight patients with HPV infection and visible anogenital warts were enrolled. Microwave or radiofrequency was used to remove visible lesions before PDT. HPV DNA detection was performed using real-time polymerase chain reaction before each PDT session and at follow-up. Treatment was halted after the patient showed two negative results for HPV DNA detection in a row. RESULTS: Of the 138 patients enrolled in the study, 72 completed treatment. Multisite HPV-infected patients required more sessions of PDT than did single-site infected patients to reach the endpoint of treatment. Compared with patients with only external CA, individuals with internal CA required more sessions to eliminate HPV infection. The total number of PDT sessions performed in the multitype HPV-infected group was significantly higher than that in the single-type infected group. Patients with non-high-risk (HR)-HPV infection required fewer PDT sessions than did those with HR-HPV infection by the end of treatment. Sixty-nine patients were followed-up for at least 6 months, only 2.9% of whom showed recurrence. CONCLUSIONS: Combined ALA-PDT and HPV DNA detection was an effective strategy for the treatment of CA. Patients with multisite and multitype HPV infection required more PDT sessions to eliminate the virus.

4.
Photodiagnosis Photodyn Ther ; 28: 183-185, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31398516

RESUMO

The facial angiofbromas due to tuberous sclerosis complex produced significant social and emotional distress for affected individuals, but there is no specific therapeutic strategy up to now. Herein, we report a case of facial angiofibromas successfully treated by 5-aminolaevulinic acid-mediated photodynamic therapy (ALA-PDT) with no recurrence for 6 years, thus providing a promising therapeutic option.

5.
BMC Infect Dis ; 19(1): 707, 2019 Aug 09.
Artigo em Inglês | MEDLINE | ID: mdl-31399065

RESUMO

BACKGROUND: Talaromyces marneffei is a thermally dimorphic fungus endemic in south-east Asia. It predominantly occurs in both immunocompromised and immunosuppressed patients and can be fatal if diagnosis and treatment are delayed. The clinical manifestations of T. marneffei infection are nonspecific and rapid diagnosis of T. marneffei infection remains challenging. CASE PRESENTATION: A 24-year-old man came to our outpatient department with the sign of common skin lesions. The lesions were cuticolor follicular papules with or without central umbilication, nodules and acne-like lesions, which are common in syringoma, steatocystoma multiplex and trichoepithelioma. A dermatoscopy examination was performed to differentiate these skin lesions. The dermatoscopic images revealed circular or quasi-circular whitish amorphous structure with a central brownish keratin plug, providing the diagnostic clues of T. marneffei infection. Therefore, a skin scrapings culture, skin biopsy and serological detection for human immunodeficiency virus (HIV) were performed. The final diagnosis of this patient was T. marneffei and HIV co-infection. CONCLUSION: Rapid diagnosis of T. marneffei infection is clinically challenging since presenting clinical manifestations are nonspecific with significant overlap with other common conditions. This case highlights that dermatoscopy is a promising tool for the rapid diagnosis of T. marneffei infection in patients with nonspecific skin lesions, assisting clinicians to avoid delayed diagnosis or misdiagnosis.


Assuntos
Dermoscopia/métodos , Micoses/diagnóstico , Talaromyces/patogenicidade , Anfotericina B/uso terapêutico , Fármacos Anti-HIV/uso terapêutico , Antifúngicos/administração & dosagem , Antifúngicos/uso terapêutico , China , Infecções por HIV/diagnóstico , Infecções por HIV/tratamento farmacológico , Humanos , Itraconazol/administração & dosagem , Itraconazol/uso terapêutico , Masculino , Micologia/métodos , Micoses/tratamento farmacológico , Adulto Jovem
6.
J Photochem Photobiol B ; 194: 46-55, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-30925276

RESUMO

Human papillomavirus (HPV) infection is linked to several diseases, the most prominent of which are cervical cancer and genital condyloma acuminatum. Previous studies have suggested an effective role for 5-aminolevulinic acid photodynamic therapy (ALA-PDT) against various cancers by the induction of autophagy and apoptosis. However, few reports have focused on the effectiveness of ALA-PDT on HPV related disorders. To identify the role of ALA-PDT in the context of HPV infection, we initially investigated 111 patients suffering from genital condyloma acuminatum. HPV viral load detected before and after ALA-PDT treatment was compared during this procedure; a significant difference was noted. HeLa (HPV18) cells were exposed to ALA-PDT in vitro to further explore the underlying mechanisms. Western blot analysis showed that ALA-PDT induces LC3II and p62 expression, along with the up regulation of caspase-3 and cleaved caspase-3. Our study also demonstrated that ALA-PDT treatment inhibits the proliferation of HeLa cells in a dose dependent manner and effectively reduces HPV viral load via autophagy and apoptosis by regulating the Ras/Raf/MEK/ERK and PI3K/AKT/mTOR pathways. Hydroxychloroquine (HCQ), although it inhibited autophagy degradation, functioned to activate reactive oxygen species (ROS) levels of ALA-PDT to enhance the observed effect. These findings suggest strategies for the improvement of PDT efficacy in patients.


Assuntos
Morte Celular/efeitos dos fármacos , Morte Celular/efeitos da radiação , Ácidos Levulínicos/farmacologia , Papillomaviridae/fisiologia , Fotoquimioterapia , Carga Viral/efeitos dos fármacos , Carga Viral/efeitos da radiação , Apoptose/efeitos dos fármacos , Apoptose/efeitos da radiação , Autofagia/efeitos dos fármacos , Autofagia/efeitos da radiação , Proliferação de Células/efeitos dos fármacos , Proliferação de Células/efeitos da radiação , Sobrevivência Celular/efeitos dos fármacos , Sobrevivência Celular/efeitos da radiação , Relação Dose-Resposta a Droga , Relação Dose-Resposta à Radiação , MAP Quinases Reguladas por Sinal Extracelular/metabolismo , Células HeLa , Humanos , Sistema de Sinalização das MAP Quinases/efeitos dos fármacos , Sistema de Sinalização das MAP Quinases/efeitos da radiação , Papillomaviridae/efeitos dos fármacos , Papillomaviridae/efeitos da radiação , Fosfatidilinositol 3-Quinases/metabolismo , Proteínas Proto-Oncogênicas c-akt/metabolismo , Espécies Reativas de Oxigênio/metabolismo , Serina-Treonina Quinases TOR/metabolismo , Quinases raf/metabolismo , Proteínas ras/metabolismo
7.
Photodiagnosis Photodyn Ther ; 25: 460-462, 2019 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-30738225

RESUMO

5-aminolevulinic acid (ALA) mediated photodynamic therapy (PDT) is a highly effective, safe, and promising treatment for human papillomavirus (HPV)-induced anogenital condyloma acuminata (CA). Specific HPV subtypes are responsible for the pathogenesis of CA and dynamic changes in viral loads may reflect the efficiency of PDT. Here we present two cases of anogenital CA successfully treated by ALA-PDT under the guidance of HPV DNA detection including genotype identification and viral load measurement, which provided clues for unexpected intra-anal infection. Both of these male patients were free of detectable HPV at the end of the treatment and no recurrence was observed after 1 year. We conclude that the HPV DNA detection can accurately guide ALA-PDT, indicate unnoticed lesions, as well as improve and evaluate the efficiency.


Assuntos
Ácido Aminolevulínico/uso terapêutico , Condiloma Acuminado/tratamento farmacológico , Papillomaviridae/genética , Infecções por Papillomavirus/tratamento farmacológico , Fotoquimioterapia/métodos , Adulto , Condiloma Acuminado/virologia , DNA Viral , Genótipo , Humanos , Lasers de Gás , Masculino , Pessoa de Meia-Idade , Infecções por Papillomavirus/genética , Fármacos Fotossensibilizantes/uso terapêutico , Reação em Cadeia da Polimerase , Carga Viral
8.
Photodiagnosis Photodyn Ther ; 26: 13-14, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-30769166

RESUMO

Chromoblastomycosis is a long-term fungal infection of the skin and subcutaneous tissue, usually presenting as partial hypertrophic and warty plaques. Effective treatment is necessary to control the development of lesions, especially in patients with associated diseases. But till now, the treatment is still a challenge. Photodynamic therapy (PDT) is an efficient and non-invasive treatment option. Here, we reported the case of a 52-year-old male with refractory chromoblastomycosis and leukopenia, who was successfully treated with 5-aminolevulinic acid-based PDT (ALA-PDT). A complete cure, confirmed by clinical improvement and mycological detection, was achieved after six sessions of every-other-week treatment. Post six months follow up no recurrence was observed. The case here suggests that ALA-PDT is a valuable anti-infective therapy for refractory chromoblastomycosis.


Assuntos
Ácido Aminolevulínico/uso terapêutico , Cromoblastomicose/tratamento farmacológico , Fotoquimioterapia/métodos , Fármacos Fotossensibilizantes/uso terapêutico , Humanos , Perna (Membro)/microbiologia , Leucopenia , Masculino , Pessoa de Meia-Idade
9.
Biomed Res Int ; 2019: 2523032, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31930114

RESUMO

Osteosarcoma (OS) is one of the most common malignant bone tumors in adolescents with a poor prognosis. Though miR-509-5p has been reported as a tumor suppressor in several human cancers, the role of miR-509-5p in OS remains unclear. In this study, our result of real-time PCR (RT-PCR) showed that the expression of miR-509-5p was significantly decreased in OS tissues and cell lines. Overexpression of miR-509-5p significantly suppressed cell proliferation and invasion in OS cell lines. Moreover, we identified tribbles homolog 2 (TRIB2) as the direct target of miR-509-5p. Knockdown of TRIB2 could inhibit the malignant capacity of OS cells. At last, we reported that TRIB2 could inhibit the bioactivity of the tumor suppressor gene p21 via blocking its transcriptional activity. Collectively, our study revealed that miR-509-5p functions as a tumor suppressor by targeting TRIB2 in OS and thus could affect the activity of p21, suggesting that miR-509-5p is a novel preventive intervention for OS patients.

14.
Mol Med Rep ; 18(1): 550-556, 2018 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-29749496

RESUMO

Expression of microRNA-338-3p (miR-338-3p) was aberrantly elevated in pemphigus vulgaris (PV), although its role in PV is still unknown. The present study investigated the functional role and possible molecular mechanisms of miR-338-3p in PV. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was performed to detect miR-338-3p expression in peripheral blood mononuclear cells (PBMCs) from patients with PV. Correlation with disease severity and anti-desmoglein 3 antibody (anti-Dsg3) titers were analyzed in patients with PV. The effects of overexpression and knockdown of miR-338-3p expression in PBMCs and effects on Th1 and Th2 cytokines were also examined using ELISA. The luciferase reporter analysis, RT-qPCR and western blot analysis were applied to investigate potential and functional target genes. The data showed that miR-338-3p expression was significantly upregulated in PV and the upregulation of miR-338-3p associated with disease severity and a high anti-Dsg3 antibody titer. Expression of miR-338-3p/mimic in healthy PBMCs significantly downregulated Th1 cytokine (IFN-γ) and upregulated Th2 cytokines (IL-4 and IL-10), whereas knockdown of miR-338-3p expression in PBMCs from patients with PV induced the reverse effects. Overexpression of miR-338-3p suppressed cell viability. Luciferase reporter, RT-qPCR and western blot assays idnicated that TNFR1-associated death domain protein (TRADD) was the direct and functional target of miR-338-3p. Increased expression of miR-338-3p contributed to the production of Dsg3 antibody by inhibiting TRADD expression to induce an imbalance in Th1/Th2 cell functions. Taken together, this study suggests that miR-338-3p may be used as a potential therapeutic target for PV treatment.


Assuntos
Autoanticorpos , Desmogleína 3/imunologia , Leucócitos Mononucleares/metabolismo , MicroRNAs/metabolismo , Pênfigo/metabolismo , Proteína de Domínio de Morte Associada a Receptor de TNF , Adulto , Idoso , Feminino , Humanos , Leucócitos Mononucleares/imunologia , Masculino , Pessoa de Meia-Idade , Pênfigo/imunologia , Células Th1 , Células Th2
15.
J Environ Manage ; 220: 173-182, 2018 Aug 15.
Artigo em Inglês | MEDLINE | ID: mdl-29778953

RESUMO

In this work, a series of flower-like CuAl layered double hydroxides (LDHs) and hierarchical CuAl/carbon fiber-LDH (CuAl/CF-LDH) materials were synthesized, and these materials were used as catalysts for the degradation of ammonia nitrogen from simulated wastewater. The morphologies and structures of the materials were characterized using scanning electron microscopy (SEM), Fourier transform infrared (FTIR) spectroscopy, Raman spectroscopy (RS), X-ray diffraction (XRD), and the Brunauer-Emmett-Teller (BET) technique. The effects of the catalyst and H2O2 loading dosages, reaction temperature, pH, Cu/Al ratio of the samples, and contact time on the degradation process were investigated by degrading ammonia nitrogen under different conditions, and the possible degradation mechanism was discussed. CuAl/CF-LDH exhibited more effectively catalytically degradation of ammonia nitrogen than others as-prepared samples, and removal efficiency reached 99.7% under the optimized conditions. The reusing capability and stability of the materials were studied. Meanwhile, the versatility of the materials was investigated by testing their performance in the absorption of azo dye, the highest removal efficiency was found to be 99.28%. The prepared materials are promising for use as effective catalysts for the degradation of ammonia nitrogen from wastewater.


Assuntos
Amônia , Carbono , Águas Residuárias/química , Adsorção , Fibra de Carbono , Peróxido de Hidrogênio , Hidróxidos , Cinética , Nitrogênio
16.
J Drug Target ; 26(9): 793-805, 2018 11.
Artigo em Inglês | MEDLINE | ID: mdl-29334266

RESUMO

BACKGROUND: Breast cancer is an alarming global public health problem and a main cause of cancer-related death in women. Systemic chemotherapy is the most widely used treatment for breast cancer. However, current chemotherapy treatments are far from desirable due to poor targeting specificity, severe side effects and vasculogenic mimicry (VM). PURPOSE: Hyaluronic acid (HA)-modified daunorubicin plus honokiol (HNK) cationic liposomes were prepared and characterised for treatment of breast cancer by eliminating VM. METHODS: HA-modified daunorubicin plus HNK cationic liposomes were prepared by a thin-film hydration method. Evaluations were performed on MCF-7 cells and MDA-MB-435S cells, which are human breast cancer cells, and xenografts of MDA-MB-435S cells. RESULTS: In vitro results revealed that the HA-modified daunorubicin plus HNK cationic liposomes enhanced the cellular uptake and destroyed VM channels. In vivo results demonstrated that the liposomes prolonged the circulation time in the blood, obviously accumulated in the tumour region, and enhanced the overall anticancer effects. Action mechanisms were related to down-regulation of VM protein indicators including FAK, EphA2, MMP-2 and MMP-9. CONCLUSIONS: The prepared HA-modified daunorubicin plus HNK cationic liposomes may serve as a promising therapeutic strategy for the treatment of breast cancer.


Assuntos
Compostos de Bifenilo/química , Neoplasias da Mama/tratamento farmacológico , Daunorrubicina/química , Daunorrubicina/uso terapêutico , Ácido Hialurônico/química , Lignanas/química , Lipossomos , Animais , Neoplasias da Mama/patologia , Cátions , Feminino , Humanos , Células MCF-7 , Camundongos , Neovascularização Patológica
17.
Artif Cells Nanomed Biotechnol ; 46(sup1): 616-628, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-29381101

RESUMO

Tumor invasion is considered a major promoter in the initiation of tumor metastasis, which is supposed to cause most cancer-related deaths. In the present study, octreotide (OCT)-modified daunorubicin plus dihydroartemisinin liposomes were developed and characterized. Evaluations were undertaken on breast cancer MDA-MB-435S cells and MDA-MB-435S xenografts nude mice. The liposomes were ∼100 nm in size with a narrow polydispersity index. In vitro results showed that the OCT-modified daunorubicin plus dihydroartemisinin liposomes could enhance cytotoxicity and cellular uptake by OCT-SSTRs (somatostatin receptors)-mediated active targeting, block on tumor cell wound healing and migration by incorporating dihydroartemisinin. The action mechanism might be related to regulations on E-cadherin, α5ß1-integrin, TGF-ß1, VEGF and MMP2/9 in breast cancer cells. In vivo, the liposomes displayed a prolonged circulating time, more accumulation in tumor location, and a robust overall antitumor efficacy with no obvious toxicity at the test dose in MDA-MB-435S xenograft mice. In conclusion, the OCT-modified daunorubicin plus dihydroartemisinin liposomes could prevent breast cancer invasion, hence providing a possible strategy for treatment of metastatic breast cancer.


Assuntos
Artemisininas/química , Artemisininas/farmacologia , Neoplasias da Mama/patologia , Daunorrubicina/química , Daunorrubicina/farmacologia , Lipossomos/química , Octreotida/química , Sulfato de Amônio/química , Animais , Artemisininas/metabolismo , Transporte Biológico , Movimento Celular/efeitos dos fármacos , Daunorrubicina/metabolismo , Humanos , Bicamadas Lipídicas/química , Células MCF-7 , Camundongos , Invasividade Neoplásica , Metástase Neoplásica , Polietilenoglicóis/química , Cicatrização/efeitos dos fármacos , Ensaios Antitumorais Modelo de Xenoenxerto
18.
Bioresour Technol ; 249: 924-934, 2018 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-29145119

RESUMO

Amine-functionalized magnetic bamboo-based activated carbon (AFM-BAC) derived from bamboo products wastes were employed for effective adsorption of fluoroquinolone antibiotics ciprofloxacin (CIP) and norfloxacin (NOR) through batch processing. The effects of factors on the adsorption of both antibiotics were studied. The studies of various factors influencing the adsorption behavior indicated that the maximum adsorption capacities for two antibiotics adsorption were observed in weakly acidic condition and the adsorption amounts of two antibiotics increased with the increase of zwitterionic form, implying the importance of zwitterionic form, and the adsorption process is spontaneous and endothermic. The result of date indicated that adsorption of both two antibiotics onto the AFM-BAC better fits Langmuir isotherm model. The saturated magnetization of AFM-BAC reached 8.55 emu g-1. A fixed-bed column adsorption with a bench-scale was carried out. Desorption and regeneration experiments showed that the AFM-BAC for both antibiotics could remain above 80% after five consecutive recycling cycles.


Assuntos
Ciprofloxacino , Norfloxacino , Poluentes Químicos da Água , Purificação da Água , Adsorção , Aminas , Carbono , Carvão Vegetal
19.
J Liposome Res ; 28(3): 236-248, 2018 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-28480778

RESUMO

CONTEXT: Non-small cell lung carcinoma (NSCLC) is a type of epithelial lung cancer that accounts for approximately 80-85% of lung carcinoma cases. Chemotherapy for the NSCLC is unsatisfactory due to multidrug resistance, nonselectively distributions and the accompanying side effects. OBJECTIVE: The objective of this study was to develop a kind of PTD modified paclitaxel anti-resistant liposomes to overcome these chemotherapy limitations. METHOD: The studies were performed on LLT cells and resistant LLT cells in vitro and on NSCLC xenograft mice in vivo, respectively. RESULTS AND DISCUSSION: In vitro results showed that the liposomes with suitable physicochemical characteristics could significantly increase intracellular uptake in both LLT cells and resistant LLT cells, evidently inhibit the growth of cancer cells, and clearly induce the apoptosis of resistant LLT cells. Studies on resistant LLT cells xenograft mice demonstrated that the liposomes magnificently enhanced the anticancer efficacy in vivo. Involved action mechanisms were down-regulation of adenosine triphosphate binding cassette transporters on resistant LLT cells, and activation of the apoptotic enzymes (caspase 8/9/3). CONCLUSION: The PTD modified paclitaxel anti-resistant liposomes may provide a promising strategy for treatment of the drug-resistant non-small cell lung cancer.


Assuntos
Antineoplásicos/química , Carcinoma Pulmonar de Células não Pequenas/tratamento farmacológico , Resistencia a Medicamentos Antineoplásicos/efeitos dos fármacos , Lipossomos/química , Neoplasias Pulmonares/tratamento farmacológico , Paclitaxel/química , Animais , Antineoplásicos/farmacologia , Antineoplásicos/uso terapêutico , Apoptose/efeitos dos fármacos , Linhagem Celular Tumoral , Portadores de Fármacos , Liberação Controlada de Fármacos , Humanos , Masculino , Camundongos Endogâmicos C57BL , Oligopeptídeos/química , Paclitaxel/farmacologia , Paclitaxel/uso terapêutico , Tamanho da Partícula , Propriedades de Superfície , Tamoxifeno/química , Tamoxifeno/farmacologia , Ensaios Antitumorais Modelo de Xenoenxerto
20.
Rejuvenation Res ; 21(1): 44-52, 2018 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-28629266

RESUMO

Natural compound polygonatum sibiricum polysaccharide (PSP) has a variety of biological actions such as reducing blood fat, antitumor and antioxidant activities, and enhancing immune function. Our previous study has revealed that PSP can promote osteoblastic (OB) differentiation in bone mesenchymal stem cells by increasing nuclear accumulation of ß-catenin. This study was designed to investigate that PSP can upregulate nuclear ß-catenin, which was prevented by inhibiting the glycogen synthase kinase 3ß (GSK-3ß) activity, by effectively activating Wnt signaling independent of low-density lipoprotein receptor-related protein 5 (LRP5). We showed that PSP reduced the level of GSK-3ß, which phosphorylates and destabilizes nuclear ß-catenin through extracellular signal-regulated kinase (ERK) signaling pathway. Taken together, these findings demonstrate that PSP promotes OB differentiation and mineralization in vitro through the ERK/GSK-3ß/ß-catenin signaling pathways. This study may aid in the development of a therapeutic approach utilizing PSP for the enhancement of bone health and prevention of osteoporosis.


Assuntos
Diferenciação Celular/efeitos dos fármacos , MAP Quinases Reguladas por Sinal Extracelular/metabolismo , Glicogênio Sintase Quinase 3 beta/metabolismo , Osteoblastos/citologia , Polygonatum/química , Polissacarídeos/farmacologia , Transdução de Sinais , beta Catenina/metabolismo , Animais , Proteína-5 Relacionada a Receptor de Lipoproteína de Baixa Densidade/metabolismo , Camundongos Endogâmicos C57BL , Osteoblastos/efeitos dos fármacos , Osteoblastos/metabolismo , Transdução de Sinais/efeitos dos fármacos , Células-Tronco/efeitos dos fármacos , Células-Tronco/metabolismo , Via de Sinalização Wnt/efeitos dos fármacos
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA