Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 24
Filtrar
Mais filtros










Intervalo de ano de publicação
1.
Preprint | SciELO Preprints | ID: pps-17473

RESUMO

Commercially available preparations of methionyl-human BMP-2 and CHO-derived hBMP-2, which belongs to the transforming growth factor ß (TGF-ß) superfamily, were used for a complete characterization. This protein is an extremely efficient osteoinductor that plays an important role during bone regeneration and embryonic development. Characterization was carried out via SDS-PAGE and Western blotting, followed by reversed-phase HPLC, size-exclusion HPLC and MALDI-TOF-MS. The classical in vitro bioassay, based on the induction of alkaline phosphatase activity in C2C12 cells, confirmed that hBMP-2 biological activity is mostly related to the dimeric form, being ~ 4-fold higher for the CHO-derived glycosylated form when compared with the E. coli counterpart. The E. coli-derived met-hBMP-2 has shown, by MALDI-TOF-MS, a large presence of the bioactive dimer. A more complex molecular mass (MM) distribution was found for the CHO-derived product, whose exact MM has never been reported because of its variable glycosylation. A method based on RP-HPLC was set up, allowing a quantitative and qualitative hBMP-2 determination even directly on ongoing culture media. Considering that hBMP-2 is highly unstable, presenting moreover an extremely high aggregate value, we believe that these data pave the way to a necessary characterization of this important factor when synthesized by DNA recombinant techniques in different types of hosts.


Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

2.
AMB Express ; 10: 34, 2020.
Artigo | Sec. Est. Saúde SP, SESSP-IBPROD, Sec. Est. Saúde SP | ID: but-ib17473

RESUMO

Commercially available preparations of methionyl-human BMP-2 and CHO-derived hBMP-2, which belongs to the transforming growth factor ß (TGF-ß) superfamily, were used for a complete characterization. This protein is an extremely efficient osteoinductor that plays an important role during bone regeneration and embryonic development. Characterization was carried out via SDS-PAGE and Western blotting, followed by reversed-phase HPLC, size-exclusion HPLC and MALDI-TOF-MS. The classical in vitro bioassay, based on the induction of alkaline phosphatase activity in C2C12 cells, confirmed that hBMP-2 biological activity is mostly related to the dimeric form, being ~ 4-fold higher for the CHO-derived glycosylated form when compared with the E. coli counterpart. The E. coli-derived met-hBMP-2 has shown, by MALDI-TOF-MS, a large presence of the bioactive dimer. A more complex molecular mass (MM) distribution was found for the CHO-derived product, whose exact MM has never been reported because of its variable glycosylation. A method based on RP-HPLC was set up, allowing a quantitative and qualitative hBMP-2 determination even directly on ongoing culture media. Considering that hBMP-2 is highly unstable, presenting moreover an extremely high aggregate value, we believe that these data pave the way to a necessary characterization of this important factor when synthesized by DNA recombinant techniques in different types of hosts.

3.
Int J Implant Dent ; 3(1): 18, 2017 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-28474322

RESUMO

BACKGROUND: It is still unclear whether enamel matrix proteins (EMD) as adjunct to bone grafting enhance bone healing. This study compared histomorphometrically maxillary sinus floor augmentation (MSFA) with ß-TCP/HA in combination with or without EMD in humans. METHODS: In ten systemically healthy patients needing bilateral MSFA, one side was randomly treated using ß-TCP/HA mixed with EMD (BC + EMD) and the other side using only ß-TCP/HA (BC). After 6 months, biopsies were harvested from grafted areas during implant installation, being histologically and histomorphometrically analyzed. Differences between the groups considering new bone formation, soft tissues, and remaining BC were statistically evaluated. RESULTS: All patients showed uneventful healing after MSFA, and dental implant installation was possible in all patients after 6 months. Histological analysis showed newly formed bone that was primarily woven in nature; it was organized in thin trabeculae, and it was occasionally in contact with residual bone substitute particles, which appeared in various forms and sizes and in advanced stage of degradation. Mean bone area was 43.4% (CI95 38.9; 47.8) for the BC group and 43.0% (CI95 36.6; 49.5) for the BC + EMD group. Mean soft tissue area was 21.3% (CI95 16.5; 26.2) for BC group and 21.5% (CI95 17.7; 25.3) for BC + EMD group, while the remaining biomaterial was 35.3% (CI95 36.6; 49.5) and 35.5% (CI95 29.6; 41.3) for BC and BC + EMD group, respectively. CONCLUSIONS: MSFA with BC resulted in adequate amounts of new bone formation allowing successful implant installation; adding EMD did not have a significant effect.

4.
J Clin Periodontol ; 43(12): 1200-1207, 2016 12.
Artigo em Inglês | MEDLINE | ID: mdl-27440671

RESUMO

AIM: The objective of this report was to present histological characteristics and gene expression profile of newly formed bone following horizontal augmentation of the atrophic anterior maxilla using recombinant human bone morphogenetic protein-2 in an absorbable collagen sponge carrier (rhBMP-2/ACS) versus an autogenous bone graft (ABG). METHODS: Bone core biopsies from 24 subjects participating in a randomized clinical trial were obtained at dental implant placement, 6 months following alveolar ridge augmentation using rhBMP-2/ACS (rhBMP-2 at 1.5 mg/ml; total dose 4.2 mg) or a particulate ABG harvested from the mandibular retro-molar region. A titanium mesh was used to provide wound stability and space for bone formation. Analysis included histological/histometric observations and gene expression profile of the newly formed bone. RESULTS: rhBMP-2/ACS yielded bone marrow rich in capillaries, undifferentiated cells and bone lining cells compared with the ABG (p = 0.002). Whereas no significant differences were observed in total bone fraction (p = 0.53), non-vital bone particles trapped in lamellar vital bone were observed in the ABG group (p < 0.001). Real-time PCR showed greater BMP-2 and RUNX2 expression for rhBMP-2/ACS over the ABG (p = 0.001 and 0.0021, respectively), while the ABG exhibited greater expression of RANKL:OPG, BSP and OPN over rhBMP-2/ACS (p = 0.01, 0.005 and 0.0009, respectively). CONCLUSIONS: Our observations suggest that formative biological processes explain bone formation following implantation of rhBMP-2/ACS, whereas remodelling, resorptive/formative processes, characterizes sites receiving ABGs.


Assuntos
Maxila , Aumento do Rebordo Alveolar , Proteína Morfogenética Óssea 2 , Proteínas Morfogenéticas Ósseas , Colágeno , Humanos , Proteínas Recombinantes , Transcriptoma , Fator de Crescimento Transformador beta
5.
J Pediatr Surg ; 50(5): 842-8, 2015 May.
Artigo em Inglês | MEDLINE | ID: mdl-25783315

RESUMO

BACKGROUND/PURPOSE: Congenital diaphragmatic hernia (CDH) is a defect that presents high mortality because of pulmonary hypoplasia and hypertension. Mechanical ventilation changes signaling pathways, such as nitric oxide and VEGF in the pulmonary arterioles. We investigated the production of NOS2 and NOS3 and expression of VEGF and its receptors after ventilation in rat fetuses with CDH. METHODS: CDH was induced by Nitrofen. The fetuses were divided into 6 groups: 1) control (C); 2) control ventilated (CV); 3) exposed to nitrofen (N-); 4) exposed to nitrofen ventilated (N-V), 5) CDH and 6) CDH ventilated (CDHV). Fetuses were harvested and ventilated. We assessed body weight (BW), total lung weight (TLW), TLW/BW ratio, the median pulmonary arteriolar wall thickness (MWT). We analyzed the expression of NOS2, NOS3, VEGF and its receptors by immunohistochemistry and Western blotting. RESULTS: BW, TLW, and TLW/BW ratio were greater on C than on N- and CDH (p<0.05). The MWT was higher in CDH than in CDHV (p<0.001). CDHV showed increased expression of NOS3 (p<0.05) and VEGFR1 (p<0.05), but decreased expression of NOS2 (p<0.05) and VEGFR2 (p<0.001) compared to CDH. CONCLUSION: Ventilation caused pulmonary vasodilation and changed the expression of NOS and VEGF receptors.


Assuntos
Hérnia Diafragmática/metabolismo , Hérnias Diafragmáticas Congênitas/metabolismo , Óxido Nítrico Sintase/metabolismo , Respiração Artificial , Fator A de Crescimento do Endotélio Vascular/metabolismo , Vasodilatação/fisiologia , Animais , Animais Recém-Nascidos , Modelos Animais de Doenças , Feminino , Hérnia Diafragmática/induzido quimicamente , Hérnia Diafragmática/fisiopatologia , Ratos , Ratos Sprague-Dawley
6.
Clin Implant Dent Relat Res ; 17 Suppl 1: e192-201, 2015 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-24102703

RESUMO

PURPOSE: The aim of this systematic review was to evaluate clinical and safety data for recombinant human bone morphogenetic protein-2 (rhBMP-2) in an absorbable collagen sponge (ACS) carrier when used for alveolar ridge/maxillary sinus augmentation in humans. MATERIALS AND METHODS: Clinical studies/case series published 1980 through June 2012 using rhBMP-2/ACS were searched. Studies meeting the following criteria were considered eligible for inclusion: >10 subjects at baseline and maxillary sinus or alveolar ridge augmentation not concomitant with implant placement. RESULTS: Seven of 69 publications were eligible for review. rhBMP-2/ACS yielded clinically meaningful bone formation for maxillary sinus augmentation that would allow placement of regular dental implants without consistent differences between rhBMP-2 concentrations. Nevertheless, the statistical analysis showed that sinus augmentation following autogenous bone graft was significantly greater (mean bone height: 1.6 mm, 95% CI: 0.5-2.7 mm) than for rhBMP-2/ACS (rhBMP-2 at 1.5 mg/mL). In extraction sockets, rhBMP-2/ACS maintained alveolar ridge height while enhancing alveolar ridge width. Safety reports did not represent concerns for the proposed indications. CONCLUSIONS: rhBMP-2/ACS appears a promising alternative to autogenous bone grafts for alveolar ridge/maxillary sinus augmentation; dose and carrier optimization may expand its efficacy, use, and clinical application.


Assuntos
Aumento do Rebordo Alveolar/métodos , Proteína Morfogenética Óssea 2/uso terapêutico , Osteogênese/efeitos dos fármacos , Levantamento do Assoalho do Seio Maxilar/métodos , Fator de Crescimento Transformador beta/uso terapêutico , Implantes Absorvíveis , Colágeno , Humanos , Proteínas Recombinantes/uso terapêutico
7.
PLoS One ; 9(8): e103566, 2014.
Artigo em Inglês | MEDLINE | ID: mdl-25084016

RESUMO

The epididymis has an important role in the maturation of sperm for fertilization, but little is known about the epididymal molecules involved in sperm modifications during this process. We have previously described the expression pattern for an antigen in epididymal epithelial cells that reacts with the monoclonal antibody (mAb) TRA 54. Immunohistochemical and immunoblotting analyses suggest that the epitope of the epididymal antigen probably involves a sugar moiety that is released into the epididymal lumen in an androgen-dependent manner and subsequently binds to luminal sperm. Using column chromatography, SDS-PAGE with in situ digestion and mass spectrometry, we have identified the protein recognized by mAb TRA 54 in mouse epididymal epithelial cells. The ∼65 kDa protein is part of a high molecular mass complex (∼260 kDa) that is also present in the sperm acrosomal vesicle and is completely released after the acrosomal reaction. The amino acid sequence of the protein corresponded to that of albumin. Immunoprecipitates with anti-albumin antibody contained the antigen recognized by mAb TRA 54, indicating that the epididymal molecule recognized by mAb TRA 54 is albumin. RT-PCR detected albumin mRNA in the epididymis and fertilization assays in vitro showed that the glycoprotein complex containing albumin was involved in the ability of sperm to recognize and penetrate the egg zona pellucida. Together, these results indicate that epididymal-derived albumin participates in the formation of a high molecular mass glycoprotein complex that has an important role in egg fertilization.


Assuntos
Albuminas/metabolismo , Epididimo/metabolismo , Glicoproteínas/metabolismo , Óvulo/metabolismo , Espermatozoides/metabolismo , Animais , Anticorpos Monoclonais , Eletroforese em Gel de Poliacrilamida , Feminino , Fertilização/fisiologia , Immunoblotting , Imunoprecipitação , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Óvulo/fisiologia , Espectrometria de Massas por Ionização por Electrospray , Espermatozoides/fisiologia
8.
Clin Oral Implants Res ; 25(2): 226-33, 2014 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-23346871

RESUMO

OBJECTIVES: To compare autogenous bone (AT) and fresh-frozen allogeneic bone (AL) in terms of histomorphometrical graft incorporation and implant osseointegration after grafting for lateral ridge augmentation in humans. MATERIALS AND METHODS: Thirty-four patients were treated with either AL (20 patients) or AT (14 patients) onlay grafts. During implant installation surgery 6 months after grafting, cylindrical biopsies were harvested perpendicularly to the lateral aspect of the augmented alveolar ridge. Additionally, titanium mini-implants were installed in the grafted regions, also perpendicularly to the ridge; these were biopsied during second-stage surgery. Histological/histomorphometric analysis was performed using decalcified and non-decalcified sections. RESULTS: Histological analysis revealed areas of necrotic bone (NcB) occasionally in contact with or completely engulfed by newly formed vital bone (VB) in both AT and AL groups (55.9 ± 27.6 vs. 43.1 ± 20.3, respectively; P = 0.19). Statistically significant larger amounts of VB (27.6 ± 17.5 vs. 8.4 ± 4.9, respectively; P = 0.0002) and less soft connective tissue (ST) (16.4 ± 15.6 vs. 48.4 ± 18.1, respectively; P ≤ 0.0001) were seen for AT compared with AL. No significant differences were observed between the groups regarding both bone-to-implant contact (BIC) and the bone area between implant threads (BA) on the mini-implant biopsies. CONCLUSION: Allogeneic bone block grafts may be an option in cases where a limited amount of augmentation is needed, and the future implant can be expected confined within the inner aspect of the bone block. However, the clinical impact of the relatively poor graft incorporation on the long-term performance of oral implants placed in AL grafts remains obscure.


Assuntos
Aumento do Rebordo Alveolar/métodos , Transplante Ósseo/métodos , Osseointegração , Adulto , Idoso , Biópsia , Implantação Dentária Endo-Óssea , Implantes Dentários , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Transplante Autólogo , Transplante Homólogo , Resultado do Tratamento
9.
J Clin Periodontol ; 40(10): 968-75, 2013 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-23998375

RESUMO

AIM: To compare the effect of recombinant human bone morphogenetic protein-2 (rhBMP-2) in an absorbable collagen sponge carrier (ACS) with autogenous bone graft for augmentation of the edentulous atrophic anterior maxilla. METHODS: Twenty-four subjects were enrolled in a randomized, controlled, parallel-group, open-label clinical trial. Subjects either received rhBMP-2/ACS (1.5 mg/ml) or particulated autogenous bone harvested from the mandibular retromolar region. A titanium-mesh was used to provide space and wound stability. A guide was used to standardize clinical recordings using an analogue caliper. Alveolar ridge width was also assessed using cone-beam computed tomography. RESULTS: rhBMP-2/ACS yielded significantly greater radiographic horizontal bone gain compared with autogenous bone graft at immediate subcrestal levels (1.5 ± 0.7 versus 0.5 ± 0.9 mm; p = 0.01); non-significant differences were observed at mid- (2.9 ± 0.8 versus 2.9 ± 0.9 mm; p = 0.98) and apical (1.7 ± 0.9 versus 1.8 ± 1.1 mm; p = 0.85) crestal levels. No significant differences in clinical horizontal bone gain were observed at 6 months between rhBMP-2/ACS and autogenous bone graft (3.2 ± 0.9 mm versus 3.7 ± 1.4 mm; p = 0.31). Sixty-two implants were placed after 6 month of healing with no significant differences between groups for number of implants, implant size, primary stability and survival. CONCLUSIONS: rhBMP-2/ACS appears a realistic alternative for augmentation of the edentulous atrophic anterior maxilla.


Assuntos
Aumento do Rebordo Alveolar/métodos , Autoenxertos/transplante , Proteína Morfogenética Óssea 2/uso terapêutico , Transplante Ósseo/métodos , Maxila/cirurgia , Fator de Crescimento Transformador beta/uso terapêutico , Implantes Absorvíveis , Adulto , Processo Alveolar/diagnóstico por imagem , Atrofia , Materiais Biocompatíveis/química , Colágeno , Tomografia Computadorizada de Feixe Cônico/métodos , Implantação Dentária Endo-Óssea/métodos , Implantes Dentários , Portadores de Fármacos , Feminino , Seguimentos , Humanos , Arcada Parcialmente Edêntula/diagnóstico por imagem , Arcada Parcialmente Edêntula/cirurgia , Masculino , Maxila/diagnóstico por imagem , Pessoa de Meia-Idade , Osteogênese/fisiologia , Proteínas Recombinantes/uso terapêutico , Telas Cirúrgicas , Titânio/química
10.
Acta Cir Bras ; 28 Suppl 1: 13-8, 2013.
Artigo em Inglês | MEDLINE | ID: mdl-23381818

RESUMO

PURPOSE: To evaluate the expression of myosin in muscle fibers of the diaphragm in experimental congenital diaphragmatic hernia (CDH). METHODS: Fetuses of pregnant rats were divided into four groups: External Control (EC), composed of non-manipulated rats; Nitrofen, composed of pregnant rats that received 100 mg of nitrofen (2,4-dichloro-4'nitrodiphenyl ether) diluted in olive oil on gestational day (GD) 9.5, whose fetuses developed CDH (N+) or not (N-), and Olive Oil Placebo (OO), composed of pregnant rats that received the oil on the same GD. The fetuses were collected on GD 18.5, 19.5, 20.5 and 21.5 (term = 22 days). We obtained body weight (BW) and photographed the diaphragm area (DA), hernia area (HA) and subsequent calculated the HA/DA ratio in N+ group. Samples of Diaphragm muscle were processed for histological staining with H/E and immunohistochemistry (IHQ) for myosin. RESULTS: The fetuses of N- and N+ groups had decreased BW and DA compared to EC and OO groups (p < 0.001). HA was decreased on GD 18.5 compared to 21.5 (p < 0.001) and the HA/DA ratio showed no difference. IHQ showed decreased expression of myosin in nitrofen groups. CONCLUSION: CDH induced by nitrofen model contributes to the understanding of muscularization in the formation of the diaphragm where the myosin expression is decreased.


Assuntos
Hérnias Diafragmáticas Congênitas , Miosinas/metabolismo , Praguicidas/toxicidade , Éteres Fenílicos/toxicidade , Animais , Modelos Animais de Doenças , Feminino , Hérnia Diafragmática/induzido quimicamente , Hérnia Diafragmática/embriologia , Hérnia Diafragmática/patologia , Imuno-Histoquímica , Gravidez , Ratos , Ratos Sprague-Dawley
11.
Clinics (Sao Paulo) ; 68(1): 59-63, 2013 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-23420158

RESUMO

OBJECTIVES: To evaluate the histological changes of tracheal cartilage and epithelium caused by tracheal occlusion at different gestational ages in a fetal rat model. METHODS: Rat fetuses were divided into two groups: a) External control, composed of non-operated rats, and b) Interventional group, composed of rats operated upon on gestational day 18.5 (term = 22 days), divided into triads: 1) Tracheal occlusion, 2) Internal control and 3) Sham (manipulated but not operated). Morphological data for body weight, total lung weight and total lung weight/body weight ratio were collected and measured on gestational days 19.5, 20.5 and 21.5. Tracheal samples were histologically processed, and epithelial, chondral and total tracheal thicknesses were measured on each gestational day. RESULTS: The tracheal occlusion group exhibited an increase in total lung weight/body weight ratio (p<0.001). Histologically, this group had a thicker epithelial thickness (p<0.05) and thinner chondral (p<0.05) and total tracheal thicknesses (p<0.001). These differences were more prominent on gestational days 20.5 and 21.5. CONCLUSION: Tracheal occlusion changed tracheal morphology, increased epithelial thickness and considerably decreased total tracheal thickness. These changes in the tracheal wall could explain the development of tracheomegaly, recently reported in some human fetuses subjected to tracheal occlusion.


Assuntos
Feto/cirurgia , Idade Gestacional , Modelos Animais , Oclusão Terapêutica/métodos , Traqueia/cirurgia , Fatores Etários , Animais , Peso Corporal , Feto/anatomia & histologia , Feto/embriologia , Pulmão/anatomia & histologia , Pulmão/embriologia , Tamanho do Órgão , Ratos , Reprodutibilidade dos Testes , Oclusão Terapêutica/efeitos adversos , Fatores de Tempo , Traqueia/anatomia & histologia , Traqueia/embriologia
12.
Clinics ; 68(1): 59-63, Jan. 2013. ilus, graf, tab
Artigo em Inglês | LILACS | ID: lil-665918

RESUMO

OBJECTIVES: To evaluate the histological changes of tracheal cartilage and epithelium caused by tracheal occlusion at different gestational ages in a fetal rat model. METHODS: Rat fetuses were divided into two groups: a) External control, composed of non-operated rats, and b) Interventional group, composed of rats operated upon on gestational day 18.5 (term = 22 days), divided into triads: 1) Tracheal occlusion, 2) Internal control and 3) Sham (manipulated but not operated). Morphological data for body weight, total lung weight and total lung weight/body weight ratio were collected and measured on gestational days 19.5, 20.5 and 21.5. Tracheal samples were histologically processed, and epithelial, chondral and total tracheal thicknesses were measured on each gestational day. RESULTS: The tracheal occlusion group exhibited an increase in total lung weight/body weight ratio (p<0.001). Histologically, this group had a thicker epithelial thickness (p<0.05) and thinner chondral (p<0.05) and total tracheal thicknesses (p<0.001). These differences were more prominent on gestational days 20.5 and 21.5. CONCLUSION: Tracheal occlusion changed tracheal morphology, increased epithelial thickness and considerably decreased total tracheal thickness. These changes in the tracheal wall could explain the development of tracheomegaly, recently reported in some human fetuses subjected to tracheal occlusion.


Assuntos
Animais , Ratos , Feto/cirurgia , Idade Gestacional , Modelos Animais , Oclusão Terapêutica/métodos , Traqueia/cirurgia , Fatores Etários , Peso Corporal , Feto/anatomia & histologia , Feto/embriologia , Pulmão/anatomia & histologia , Pulmão/embriologia , Tamanho do Órgão , Reprodutibilidade dos Testes , Fatores de Tempo , Oclusão Terapêutica/efeitos adversos , Traqueia/anatomia & histologia , Traqueia/embriologia
13.
Clin Oral Implants Res ; 24(9): 963-8, 2013 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-22697457

RESUMO

OBJECTIVES: To present some immunological aspects of fresh-frozen allogeneic bone grafting for lateral bone augmentation, based on the quantitative evaluation of IL-10, IL-1ß, IFN- γ and TNF- α in patients sera. MATERIAL AND METHODS: Thirty-three partially or totally edentulous patients received fresh-frozen allogeneic bone (AL - 20 patients) or autologous bone onlay block grafts (AT - 13 patients) prior to oral implant placement. Blood samples were collected from each patient at various time-points during a 6 month-period (baseline, 14, 30, 90 and 180 days postoperatively). Quantitative evaluation of IL-10, IL-1ß, IFN- γ and TNF- α was performed by enzyme linked immunosorbent assay (ELISA). RESULTS: For all evaluated markers and at all evaluated periods, inter-group comparisons showed no statistically significant differences between the groups, while the observed values were within normal levels. For AL-treated patients, intra-group evaluation showed statistically significant increase of TNF-α from baseline to 90 (P < 0.001) and 180 (P < 0.01) days, and from 14 to 90 (P < 0.01) and 180 (P < 0.05) days. IFN- γ showed intercalated results, with a decrease from baseline to 14 days (P < 0.05), and increase from 14 to 90 days (P < 0.001) and 180 (P < 0.05) days. No differences between the periods of evaluation were found for the AT group. CONCLUSIONS: AL grafting for lateral bone augmentation, similar to AT grafting, does not seem to challenge the immune system significantly.


Assuntos
Aumento do Rebordo Alveolar/métodos , Biomarcadores/sangue , Transplante Ósseo/métodos , Implantação Dentária Endo-Óssea/métodos , Arcada Edêntula/cirurgia , Adulto , Idoso , Ensaio de Imunoadsorção Enzimática , Feminino , Humanos , Interferon gama/sangue , Interleucina-10/sangue , Interleucina-1beta/sangue , Masculino , Pessoa de Meia-Idade , Retalhos Cirúrgicos , Resultado do Tratamento , Fator de Necrose Tumoral alfa/sangue
14.
Clin Implant Dent Relat Res ; 15(2): 262-70, 2013 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-21834861

RESUMO

BACKGROUND: The relationship between the immune response and red and white blood cell homeostasis is cited in literature, but no studies regarding the balance of these cell populations following maxillary bone-graft surgeries can be found. AIM: The aim of this study was to evaluate the possible impairments in the blood cell balance following fresh-frozen allogeneic bone-graft augmentation procedures in patients who needed maxillary reconstruction prior to implants. MATERIAL AND METHODS: From 33 patients elected to onlay bone grafting procedures, 20 were treated with fresh-frozen bone allografts and 13 with autologous bone grafts. Five blood samples were collected from each patient in a 6-month period (baseline: 14, 30, 90, and 180 days postsurgery), and the hematological parameters (erythrogram, leukogram, and platelets count) were accessed. RESULTS: All evaluated parameters were within the reference values accepted as normal, and significant differences were found for the eosinophils count when comparing the treatments (30 days, p = .035) and when comparing different periods of evaluation (allograft-treated group, baseline × 180 days, p ≤ .05 and 90 × 180 days, p ≤ .01; autograft-treated group, 30 × 90 days, p ≤ .05 and 30 × 180 days, p ≤ .05). CONCLUSIONS: Both autologous and fresh-frozen allogeneic bone grafts did not cause any impairment in the red and white blood cell balance, based on quantitative hemogram analysis, in patients subjected to maxillary reconstruction.


Assuntos
Aloenxertos/transplante , Aumento do Rebordo Alveolar/métodos , Contagem de Células Sanguíneas/classificação , Células Sanguíneas/classificação , Transplante Ósseo/métodos , Maxila/cirurgia , Adulto , Idoso , Autoenxertos/transplante , Criopreservação/métodos , Eosinófilos/patologia , Contagem de Eritrócitos , Índices de Eritrócitos , Feminino , Seguimentos , Hematócrito , Hemoglobinas/análise , Humanos , Contagem de Leucócitos , Masculino , Pessoa de Meia-Idade , Monócitos/patologia , Contagem de Plaquetas
15.
Clin Implant Dent Relat Res ; 15(4): 490-7, 2013 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-21834864

RESUMO

BACKGROUND: In the absence of autologous bone for harvesting, fresh-frozen bone allografts turned into an alternative for bone reconstruction procedures. PURPOSE: The purpose of this study was to make a histological analysis of fresh-frozen onlay bone allografts (ALs), compared with autografts, in patients who needed maxillary reconstruction prior to dental implants placement. MATERIALS AND METHODS: Twelve patients with bone deficiencies (width inferior to 4 mm) in the sites where the implants were planned were enrolled in the study. From these, six were elected to be treated with autogenous (AT) bone grafts and six with fresh-frozen bone AL. This last group included the patients who had absence of a convenient amount of bone in donor sites. Each patient received from one to six graft blocks, totalling to 12 ATs and 17 ALs. Seven months after grafting procedures, biopsies of the grafts were made using 2-mm internal diameter trephine burs, and processed for histological analysis. One biopsy was retrieved from each patient. RESULTS: Clinically, all grafts were found to be firm in consistency and well-incorporated to the receptor bed. Histological analysis showed a large amount of necrotic bone surrounded by few spots of new-formed bone in the AL group, suggesting low rate of graft remodeling. In the AT group, an advanced stage of bone remodeling was seen. CONCLUSIONS: Human fresh-frozen bone block AL showed clinical compatibility for grafting procedures, although associated to slow remodeling process. Further studies are needed to define, at long term, the remodeling process chronology the clinical longitudinal results for fresh-frozen bone AL.


Assuntos
Aloenxertos/anatomia & histologia , Aumento do Rebordo Alveolar/métodos , Autoenxertos/anatomia & histologia , Transplante Ósseo/classificação , Maxila/cirurgia , Adulto , Aloenxertos/transplante , Autoenxertos/transplante , Biópsia/métodos , Remodelação Óssea/fisiologia , Parafusos Ósseos , Colágeno , Criopreservação/métodos , Implantação Dentária Endo-Óssea/métodos , Feminino , Seguimentos , Sobrevivência de Enxerto , Humanos , Arcada Edêntula/cirurgia , Arcada Parcialmente Edêntula/cirurgia , Masculino , Membranas Artificiais , Pessoa de Meia-Idade , Necrose , Osteócitos/citologia , Osteogênese/fisiologia , Procedimentos Cirúrgicos Reconstrutivos/métodos
16.
Acta cir. bras ; 28(supl.1): 13-18, 2013. ilus, tab
Artigo em Inglês | LILACS | ID: lil-663886

RESUMO

PURPOSE: To evaluate the expression of myosin in muscle fibers of the diaphragm in experimental congenital diaphragmatic hernia (CDH). METHODS: Fetuses of pregnant rats were divided into four groups: External Control (EC), composed of non-manipulated rats; Nitrofen, composed of pregnant rats that received 100 mg of nitrofen (2,4-dichloro-4'nitrodiphenyl ether) diluted in olive oil on gestational day (GD) 9.5, whose fetuses developed CDH (N+) or not (N-), and Olive Oil Placebo (OO), composed of pregnant rats that received the oil on the same GD. The fetuses were collected on GD 18.5, 19.5, 20.5 and 21.5 (term = 22 days). We obtained body weight (BW) and photographed the diaphragm area (DA), hernia area (HA) and subsequent calculated the HA/DA ratio in N+ group. Samples of Diaphragm muscle were processed for histological staining with H/E and immunohistochemistry (IHQ) for myosin.} RESULTS: The fetuses of N- and N+ groups had decreased BW and DA compared to EC and OO groups (p <0.001). HA was decreased on GD 18.5 compared to 21.5 (p <0.001) and the HA/DA ratio showed no difference. IHQ showed decreased expression of myosin in nitrofen groups. CONCLUSION: CDH induced by nitrofen model contributes to the understanding of muscularization in the formation of the diaphragm where the myosin expression is decreased.


OBJETIVO: Avaliar a expressão da miosina na muscularização do diafragma na hérnia diafragmática congênita (CDH) experimental. MÉTODOS: Fetos de ratas foram divididos em quatro grupos: Controle Externo (EC), composto de ratas não manipuladas; Nitrofen, composto de ratas que receberam 100 mg de nitrofen (2,4-dicloro-4'nitrodifenil éter) diluído no azeite no dia de gestação (GD) 9.5, cujos fetos desenvolveram CDH (N+) ou não (N-) e Placebo óleo de oliva (OO), composto de ratas que ingeriram apenas óleo no mesmo GD. Os fetos foram coletados com 18,5, 19,5, 20,5 e 21,5 GD (termo = 22 dias). Foi obtido o peso corporal (BW) e tiradas fotografias da área do diafragma (DA), da hérnia (HA) e calculada a relação HA/DA no grupo N+. Amostras de diafragmas foram processadas histologicamente para coloração com H/E e imunohistoquímica. RESULTADOS: Os fetos dos grupos N- e N+ tiveram BW e DA diminuídos em relação aos grupos EC e OO (p<0.001). Só houve diferença na HA entre os GD 18.5 e 21.5 (p<0.001) e a relação HA/DA não mostrou diferença entre os grupos. A imunohistoquímica mostrou menor expressão de miosina nos grupos que receberam nitrofen. CONCLUSÃO: O modelo de CDH induzida por nitrofen contribui para entender a muscularização na formação do diafragma onde a expressão da miosina está diminuída.


Assuntos
Animais , Feminino , Gravidez , Ratos , Hérnia Diafragmática/congênito , Miosinas/metabolismo , Praguicidas/toxicidade , Éteres Fenílicos/toxicidade , Modelos Animais de Doenças , Hérnia Diafragmática/induzido quimicamente , Hérnia Diafragmática/embriologia , Hérnia Diafragmática/patologia , Imuno-Histoquímica , Ratos Sprague-Dawley
17.
Rev. Assoc. Paul. Cir. Dent ; 66(2): 110-117, abr.-jun. 2012. ilus
Artigo em Português | LILACS, BBO - Odontologia | ID: lil-667461

RESUMO

A perda óssea na região anterior da maxila, por tratar-se de região estética, é um dos grandes desafios atuais da implantodontia. A estratégia mais indicada para o aumento ósseo dessa região, com o objetivo de reabilitação protética com implantes, é o emprego de enxerto ósseo autógeno. Alternativamente, outras metodologias de aumento ósseo têm sido utilizadas, tais como o enxerto ósseo homógeno, xenógeno, os biomateriais e diferentes associações destes materiais. Adicionalmente, grande atenção tem sido dada às Proteínas Ósseas Morfogenéticas (BMPs). principalmente à rh (recombinante humana) BMP-2, a qual foi caracterizada como um potente osteoindutor e alternativa em potencial ao enxerto ósseo autógeno, sem necessidade de associação com outros biomateriais. O objetivo deste estudo é apresentar um relato de caso clínico com a utilização da rhBMP-2.


The bone resorption in the anterior maxilla, due to its aesthetic importance, can be considered one of the challenges in implant dentistrv Autogenous bone graft is the most indicated bone augmentation procedure, aiming an implant supported rehabilitation .. Alternatively, some other graft procedures can be done with homogenous and xenogenous bone graft, biomaterials and different associations. Additionally to the mentioned biomaterials, the bone morphogenetic protein (BMP), specially the rhBMP-2, which was characterized as abone osteoinductor, and consecutively, a potential autogenous graft substitute, with previsibility and no necessity of association to other biomaterial. The objective of this study is to present a single case using the rhBMP-2 for bone augmentation.


Assuntos
Humanos , Masculino , Regeneração Óssea , /biossíntese , Transplante Ósseo/métodos
18.
Arq Neuropsiquiatr ; 64(3B): 787-93, 2006 Sep.
Artigo em Português | MEDLINE | ID: mdl-17057886

RESUMO

The gliotoxic ethidium bromide (EB) was used to study morphologically the macrophagic and astrocytic response under immunosuppression by cyclophosphamide (CY). Astrocyte immunoreactivity to glial fibrillary acidic protein (GFAP) and vimentin (VIM) and macrophagic immunoreactivity to ED1 were investigated after EB injection. Male Wistar rats were injected with 0.9% saline solution (group I), 0.1% BE (group II) and 0.1% EB associated with CY treatment (group III). Brainstem samples were collected from the 1st to the 21st day post-injection for GFAP, VIM and ED1 immunostaining. In groups II and III, it was observed increased immunoreactivity to GFAP and reexpression of VIM. In group II, ED1-positive cells were noted after the 2nd day and in group III, after the 3rd day. On the 14th day post-injection, it was observed a greater quantity of ED1- positive cells in group III than in group II. Apparently, CY did not change the astrocytic response pattern.


Assuntos
Astrócitos/efeitos dos fármacos , Tronco Encefálico/patologia , Ciclofosfamida/uso terapêutico , Etídio/toxicidade , Imunossupressores/uso terapêutico , Macrófagos/efeitos dos fármacos , Animais , Astrócitos/patologia , Tronco Encefálico/efeitos dos fármacos , Modelos Animais de Doenças , Imuno-Histoquímica , Macrófagos/patologia , Masculino , Ratos , Ratos Wistar , Coloração e Rotulagem
19.
Arq. neuropsiquiatr ; 64(3b): 787-793, set. 2006. ilus, tab
Artigo em Português, Inglês | LILACS | ID: lil-437150

RESUMO

O gliotóxico brometo de etídio (BE) foi utilizado para o estudo da resposta macrofágica e astrocitária sob imunossupressão com ciclofosfamida (CY). Investigou-se a imunorreatividade astrocitária à proteína glial fibrilar ácida (GFAP) e à vimentina (VIM), e a imunorreatividade macrofágica ao ED1 após injeção do BE. Foram utilizados ratos Wistar adultos injetados na cisterna basal com salina a 0,9 por cento (grupo I), BE a 0,1 por cento (grupo II) e BE a 0,1 por cento, imunossuprimidos com CY (grupo III). Fragmentos do tronco encefálico foram colhidos do 1° ao 21° dia pós-injeção para estudo imuno-histoquímico da GFAP, VIM e ED1. Nos grupos II e III, observou-se imunorreatividade aumentada para GFAP e re-expressão de VIM. No grupo II, células ED1-positivas foram observadas a partir do 2° dia e no grupo III, a partir do 3° dia. Aos 14 dias pós-injeção, havia mais células ED1-positivas no grupo III. A CY aparentemente não alterou a resposta astrocitária.


The gliotoxic ethidium bromide (EB) was used to study morphologically the macrophagic and astrocytic response under immunosuppression by cyclophosphamide (CY). Astrocyte immunoreactivity to glial fibrillary acidic protein (GFAP) and vimentin (VIM) and macrophagic immunoreactivity to ED1 were investigated after EB injection. Male Wistar rats were injected with 0.9 percent saline solution (group I), 0.1 percent BE (group II) and 0.1 percent EB associated with CY treatment (group III). Brainstem samples were collected from the 1st to the 21st day post-injection for GFAP, VIM and ED1 immunostaining. In groups II and III, it was observed increased immunoreactivity to GFAP and reexpression of VIM. In group II, ED1-positive cells were noted after the 2nd day and in group III, after the 3rd day. On the 14th day post-injection, it was observed a greater quantity of ED1- positive cells in group III than in group II. Apparently, CY did not change the astrocytic response pattern.


Assuntos
Animais , Masculino , Ratos , Astrócitos/efeitos dos fármacos , Tronco Encefálico/patologia , Ciclofosfamida/uso terapêutico , Etídio/toxicidade , Imunossupressores/uso terapêutico , Macrófagos/efeitos dos fármacos , Astrócitos/patologia , Tronco Encefálico/efeitos dos fármacos , Modelos Animais de Doenças , Imuno-Histoquímica , Macrófagos/patologia , Ratos Wistar , Coloração e Rotulagem
20.
ImplantNews ; 1(3): 255-260, maio-jun. 2004. ilus
Artigo em Português | LILACS, BBO - Odontologia | ID: lil-412363

RESUMO

Segundo a Sociedade Européia de Biomateriais, biomaterial é qualquer substância, droga, combinação de substâncias de origem natural ou sintética que pode ser usada por um período como parte de sistema tratado ou como reposição de algum tecido, órgão ou função do corpo. Contudo, hoje em dia, existe uma grande quantidade de nomenclatura sendo utilizada para os vários tipos de biomateriais, tornando difícil à comunicação e o entendimento para os profissionais da área. Assim esse trabalho tem por objetivo propor uma nomenclatura para esses materiais, bem como definir em quais situações clínicas devem ser utilizados


Assuntos
Materiais Biocompatíveis , Implantes Dentários , Classificação , Terminologia , Transplantes
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA