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1.
Medicine (Baltimore) ; 98(49): e18274, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31804367

RESUMO

Chikungunya fever (CHIKF) is an arbovirus characterized by acute fever, myalgia and polyarthralgia. Lymphedema in the lower limbs (LL) was observed in several patients during an outbreak of CHIKF in the state of Pernambuco (Brazil) in 2016. No reports on lymphatic vessels disease due to CHIKF have been described. The aim of the study was to follow lymphatic abnormalities in the LL of 16 patients with CHIKF, using lymphoscintigraphy.An observational, prospective study with patients in the acute phase of CHIKF (confirmed serological diagnosis) with LL edema submitted to clinical evaluation and lymphoscintigraphy at baseline and after 90 days.Sixteen patients (81% females) participated in this study. All patients presented with lower limb lymphedema, being 15 (94%) bilateral. Of the 31 limbs affected by lymphedema, 24 (77%) presented abnormalities in lymphatic drainage by lymphoscintigraphy. The delay to visualize pelvic lymph nodes was the most frequent lymphoscintigraphic abnormality, observed in 16 (51,6%) LL. Nine (56%) patients were clinically reevaluated after 90 days, and all 18 LL remained with lymphedema. A second lymphoscintigraphy showed persistent abnormalities in 13 (72%) of the 18 LL.CHIKF can lead to lymphedema, and lymphedema may persist or progress after 3 months of the acute phases of the disease.


Assuntos
Febre de Chikungunya/complicações , Extremidade Inferior , Linfedema/virologia , Feminino , Humanos , Linfedema/diagnóstico por imagem , Linfocintigrafia , Masculino , Pessoa de Meia-Idade , Estudos Prospectivos , Ultrassonografia
2.
Rev Col Bras Cir ; 46(5): e20192260, 2019.
Artigo em Português, Inglês | MEDLINE | ID: mdl-31859723

RESUMO

OBJECTIVE: to evaluate the use of a bacterial cellulose biopolymer film and gel dressing in the treatment of patients with ischemic wounds submitted to lower limb revascularization. METHODS: we conducted a randomized clinical trial in the Angiology and Vascular Surgery outpatient clinic of the Clinics Hospital of the Federal University of Pernambuco, between January 2017 and December 2018. We followed 24 patients after lower limb revascularization, divided into two groups: Experimental, treated with bacterial cellulose biopolymer film and gel, and Control, treated with essential fatty acids. Patients attended weekly appointments to change dressings and had their wound healing processes evaluated over a period of 90 days. RESULTS: the reduction of the ischemic wounds' areas after 30 days was 4.3cm2 (55%) on average for the experimental group, and the 5.5cm2 (48.5%) for the control group (p>0.05). The complete healing rate at 90 days was 34.8%, 50% in the experimental group and 18.2% in the control group (p=0.053). CONCLUSION: the bacterial cellulose biopolymer film associated with gel can be used as a dressing in the treatment of ischemic wounds of patients undergoing revascularization of the lower limbs.

3.
Psicopedagogia ; 36(109): 73-86, jan.-abr. 2019. graf, tab
Artigo em Português | LILACS-Express | ID: biblio-1002942

RESUMO

INTRODUÇÃO: O sono é um comportamento reversível de desligamento da percepção ao ambiente necessário para a manutenção da saúde física e cognitiva. Sua relação com o aprendizado é crucial para que o indivíduo consiga produzir aprendizado. No contexto escolar, a compreensão desse processo é essencial para garantir que as aulas sejam eficientes. Assim, este trabalho visa estudar a relação entre o cronotipo, o perfil comportamental e a efetividade das aulas práticas. MÉTODO: Para isso, foram selecionados 75 alunos de uma escola fundamental no município de Vitória de Santo Antão, Pernambuco, Brasil. Alunos do 6º ou 9º ano tiveram seu perfil comportamental de sono, ansiedade e cronotipo traçados por meio dos questionários de índice de qualidade de sono, IDATE traço-estado e o questionário de matutinidade-vespertinidade. Posteriormente, foram submetidos a três aulas práticas e ao final de cada aula foi aplicado um método avaliativo alternando entre mapa mental e questionário. RESULTADOS: Os resultados dos questionários de comportamento foram analisados conforme seus escores. Após a análise dos dados, observou-se que alunos com tendência a ansiedade e depressão obtiveram rendimentos baixos, independentemente da metodologia utilizada. Já alunos com distúrbios de sono apresentaram baixo desempenho, com exceção da metodologia de recurso multimídia aplicada para o ensino de ciências, em que, de modo geral, verificou-se um bom aproveitamento do conteúdo de ciências. Por fim, verificaram-se diferentes rendimentos nos perfis cronobiológicos dos alunos, em que alunos com perfis extremos obtiveram bons rendimentos em relação ao ensino-aprendizagem de ciências. CONCLUSÃO: Portanto, as disfunções de comportamento podem agir como um fator limitante no aprendizado, porém, dependendo do tipo de metodologia de ensino utilizada nas aulas práticas de ciências, isto pode ser atenuado.


INTRODUCTION: Sleep is a reversible behavior of the perception disconnection to the environment necessary for the maintenance of physical and cognitive health. Its relation to learning is crucial for the individual to be able to produce learning. In the school context, understanding these processes is essential to ensure that the practical classes are efficient. Thus, this study aims to study the relationship between the chronotype, the behavioral profile and the effectiveness of the practical classes. METHODS: Seventy-five students from a fundamental school, Vitória de Santo Antão, Pernambuco, Brazil, were selected. Students of the 6th or 9th levels had their behavioral profile of sleep, anxiety, and chronotype traced through the questionnaires of sleep quality index, IDATE trait-state and questionnaire of morning-evening. Subsequently, they were submitted to three practical classes and at the end was applied mental map and questionnaire to evaluate the performance. RESULTS: After analyzing the data, it was observed that students with a tendency to anxiety and depression obtained low incomes regardless of the methodology used, whereas students with sleep disorders presented low income, except for the multimedia resource methodology applied to science teaching. Finally, there were different performances in the chronobiological profiles of the students, where students with extreme profiles obtained good income to science teaching and learning. CONCLUSION: Therefore, behavioral dysfunctions may act as a limiting factor in learning, but depending on the type of methodology applied to science teaching, this can be attenuated.

4.
Rev. Enferm. Atual In Derme ; 87(Suplemento)2019.
Artigo em Português | BDENF - Enfermagem | ID: biblio-1025354

RESUMO

Objetivo: Desenvolver uma unha cirúrgica de BPCA com o propósito de produzir uma cobertura para o leito ungueal imediatamente exposto após avulsão da lâmina ungueal. Método: A unha cirúrgica de BPCA foi produzida a partir do gel de BPCA, que tem propriedades viscoelásticas e é estável em concentrações de 0,6% e 0,8%. Resultados: A comprovada biocompatibilidade e a baixa toxicidade do polissacarídeo celulósico permitem idealizar a sua utilização como um dispositivo médico, servindo como barreira mecânica, proteção do leito ungueal e remodelação da lâmina ungueal. As características físico-químicas do polissacarídeo permitem a produção de um modelo ungueal autoaderente e resistente, servindo temporariamente como substituto da lâmina ungueal. Além disso, o custo da unha cirúrgica de BPCA é acessível. Conclusão: A unha cirúrgica de BPCA parece ser uma alternativa promissora para manutenção e cicatrização do leito ungueal


Objective: Develop a surgical nail of BPCA with the purpose of producing a cover for the nail bed immediately exposed after avulsion of the nail plate. Method: The surgical nail of BPCA was produced from the BPCA gel, which has viscoelastic properties and is stable at concentrations of 0.6% and 0.8%. Results: The biocompatibility and low toxicity of the cellulosic polysaccharide allow to idealize its use as a medical device, serving as a mechanical barrier, protection of the nail bed and remodeling of the nail plate. The physico-chemical characteristics of the polysaccharide allow the production of a self-adhesive and resistant nail model, serving temporarily as a substitute for the nail plate. In addition, the cost of the surgical nail of BPCA is affordable. Conclusion: The surgical nail of BPCA appears to be a promising alternative for maintenance and healing of the nail bed


Assuntos
Humanos , Cicatrização , Biopolímeros , Saccharum , Doenças da Unha , Curativos Oclusivos
5.
Rev. Enferm. Atual In Derme ; 87(Edição Especial)2019.
Artigo em Português | BDENF - Enfermagem | ID: biblio-1025404

RESUMO

Objetivou-se monitorar a evolução do processo cicatricial das lesões por pressão (LP) em pacientes hospitalizados em Unidades de Terapia Intensiva (UTI), a partir da aplicação da cobertura de celulose bacteriana (CB). Trata-se de uma série de casos, amostra constituída por 10 pacientes internados na UTI. Foram excluídos crianças, adolescentes, e pacientes com outros tipos de lesões de pele. Foram aplicadas as escalas preditivas de Braden e a de MEASURE. Realizado monitoramento fotográfico pelo programa MOWA®. A pesquisa foi desenvolvida no setor de terapia intensiva de um hospital universitário em Recife, Pernambuco. A maioria dos pacientes era do sexo feminino (60%), com média de idade de 60±12anos. Quanto as comorbidades, 3 pacientes apresentavam diabetes mellitus (DM), 2 hipertensão arterial sistêmica (HAS), 3 DM e HAS e 2 negaram doença crônica. Sete pacientes foram acompanhados por 30 dias, em média. O risco avaliado pela escala de Braden foi de 9,66. As LP localizavam-se na região sacral, com área média de 84,48cm2 à avaliação inicial. Após 30 dias de acompanhamento observou-se redução da área média das LP (-14,7cm²). A CB funcionou como barreira física e indutor do remodelamento tecidual


The objective of this study was to monitor the evolution of the cicatricial process of pressure lesions (LP) in patients hospitalized in Intensive Care Units (ICU), from the application of bacterial cellulose (CB) coverage. This is a series of cases, a sample consisting of 10 patients hospitalized in the ICU. Children, adolescents, and patients with other types of skin lesions were excluded. Predictive scales of Braden and MEASURE were applied. Photographic monitoring carried out by the MOWA® program. The research was developed in the intensive care sector of a university hospital in Recife, Pernambuco. The majority of the patients were female (60%), with a mean age of 60 ± 12 years. As for comorbidities, 3 patients had diabetes mellitus (DM), 2 systemic arterial hypertension (SAH), 3 DM and SAH and 2 denied chronic disease. Seven patients were followed for 30 days, on average. The risk assessed by the Braden scale was 9.66. LPs were located in the sacral region, with a mean area of 84.48 cm2 at the initial evaluation. After 30 days of follow-up, we observed a reduction in the mean area of LP (-14.7 cm²). CB worked as a physical barrier and inducer of tissue remodeling


Assuntos
Humanos , Cicatrização , Celulose , Lesão por Pressão , Saccharum
6.
Int. braz. j. urol ; 44(6): 1215-1223, Nov.-Dec. 2018. tab, graf
Artigo em Inglês | LILACS-Express | ID: biblio-975663

RESUMO

ABSTRACT Purpose: The objective of the present study is to test the efficiency and practicality of a new artificial sphincter "BR - SL - AS - 904" in the control of urinary incontinence in post - PR patients and to evaluate their complications. Patients and Methods: Fifteen patients with incontinence after one year of radical prostatectomy were included prospectively. All patients underwent artificial urethral sphincter (AUS) implant "BR - SL - AS - 904" according to established technique. Independent variables such as free urinary flow, PAD weight test, ICIQ - SF score and urinary symptoms through the IPSS score were compared in different follow-up moments. Results: Patients submitted to AUS implantation did not present trans - operative or post - operative complications related to the surgical act such as: infection, hematoma, erosion or urinary retention. Device was inert to the body during the follow-up, showing an excellent adaptation of the patients, besides the easy handling. The mean age was 68.20 years 40% of the patients had systemic arterial hypertension, 6.7% diabetes mellitus, 6.7% were hypertensive and diabetic, 13.4% were hypertensive, had diabetes and hypercholesterolemia and 26.7% patients had no comorbidities. It was evidenced that the urinary flow peak during the follow-up remained stable. Decreased averages and median PAD weight test were 135.19 to 75.72 and 106.00 to 23.50, respectively. The IPSS score decreased and the quality of life increased (12.33 to 3.40 and 2.50 to 3.20 respectively). The ICQF - SF questionnaire score also showed a decrease, ranging from 16, 71 to 7.33. Conclusion: The artificial sphincter implant "BR - SL - AS 904" was reproducible, safe and effective in the control of urinary incontinence in post - PR patients.

7.
Int Braz J Urol ; 44(6): 1215-1223, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-30325613

RESUMO

PURPOSE: The objective of the present study is to test the efficiency and practicality of a new artificial sphincter "BR - SL - AS - 904" in the control of urinary incontinence in post - PR patients and to evaluate their complications. PATIENTS AND METHODS: Fifteen patients with incontinence after one year of radical prostatectomy were included prospectively. All patients underwent artificial urethral sphincter (AUS) implant "BR - SL - AS - 904" according to established technique. Independent variables such as free urinary flow, PAD weight test, ICIQ - SF score and urinary symptoms through the IPSS score were compared in different follow-up moments. RESULTS: Patients submitted to AUS implantation did not present trans - operative or post - operative complications related to the surgical act such as: infection, hematoma, erosion or urinary retention. Device was inert to the body during the follow-up, showing an excellent adaptation of the patients, besides the easy handling. The mean age was 68.20 years 40% of the patients had systemic arterial hypertension, 6.7% diabetes mellitus, 6.7% were hypertensive and diabetic, 13.4% were hypertensive, had diabetes and hypercholesterolemia and 26.7% patients had no comorbidities. It was evidenced that the urinary flow peak during the follow-up remained stable. Decreased averages and median PAD weight test were 135.19 to 75.72 and 106.00 to 23.50, respectively. The IPSS score decreased and the quality of life increased (12.33 to 3.40 and 2.50 to 3.20 respectively). The ICQF - SF questionnaire score also showed a decrease, ranging from 16, 71 to 7.33. CONCLUSION: The artificial sphincter implant "BR - SL - AS 904" was reproducible, safe and effective in the control of urinary incontinence in post - PR patients.


Assuntos
Prostatectomia/efeitos adversos , Implantação de Prótese/métodos , Incontinência Urinária/cirurgia , Esfíncter Urinário Artificial , Idoso , Estudos de Casos e Controles , Humanos , Masculino , Estudos Prospectivos , Qualidade de Vida , Resultado do Tratamento , Incontinência Urinária/etiologia
8.
Heliyon ; 4(12): e01058, 2018 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-30603694

RESUMO

The aim of this study is to evaluate if a gel of bacterial cellulose gel can revert the loss of anal resting pressure after anorectum sphincter injury in rat model, elected as a model to simulate fecal incontinence. Thirty-nine animals were equally divided into three groups: Control (CG), Sphincter injury plus Saline injection (SG) and Sphincter injury plus Bacterial Cellulose Gel injection (BCG). Anal pressure at rest was assessed for all animal in the three groups using anorectum manometry. Saline and Gel groups were subject to anorectum sphincter injury to reduce the anal pressure at rest. Fifteen days later Saline or Gel was injected into the anorectum, according to their groups. Sixty days later first manometry, the anorectum of all animals were removed and processed histologically. The CG group showed maintenance of their mean anorectal resting pressure levels; SG presented a fall in their mean anorectal resting pressure. The BCG presented a significant elevation of the mean anorectal resting pressure levels, surpassing the pressure of CG. The gel of bacterial cellulose remained at the injection site and was neovascularized, colonized by fibroblasts and dense conjunctive tissue. Those data suggest that BC can be used as a future filling agent treatment for fecal incontinence in clinical trial protocols.

9.
Rev Col Bras Cir ; 44(1): 72-80, 2017.
Artigo em Inglês, Português | MEDLINE | ID: mdl-28489214

RESUMO

Objective: to evaluate the efficacy of Bacterial Cellulose (BC) membrane dressings in the treatment of lower limb venous ulcers. Methods: we carried out a prospective, randomized, controlled study of 25 patients with chronic venous ulcer disease in the lower limbs from the Angiology and Vascular Surgery Service of the Federal University of Pernambuco Hospital and from the Salgado Polyclinic of the County Health Department, Caruaru, Pernambuco. We randomly assigned patients to two groups: control group, receiving dressings with triglyceride oil (11 patients) and experimental group, treated with BC membrane (14 patients). We followed the patients for a period of 120 days. Results: There was a reduction in the wound area in both groups. There were no infections or reactions to the product in any of the groups. Patients in the BC group showed decreased pain and earlier discontinuation of analgesic use. Conclusion: BC membrane can be used as a dressing for the treatment of varicose ulcers of the lower limbs.


Assuntos
Bandagens , Celulose/uso terapêutico , Membranas Artificiais , Úlcera Varicosa/terapia , Bactérias/metabolismo , Celulose/biossíntese , Doença Crônica , Feminino , Humanos , Perna (Membro) , Masculino , Pessoa de Meia-Idade , Estudos Prospectivos , Resultado do Tratamento
10.
J Vasc Surg Venous Lymphat Disord ; 5(3): 363-369, 2017 05.
Artigo em Inglês | MEDLINE | ID: mdl-28411704

RESUMO

OBJECTIVE: The contralateral limbs of patients with unilateral lymphedema in the lower limbs (LLs) can exhibit abnormal lymphatic circulation, even in the absence of lymphedema. This idea is based on a number of reports that have studied isolated cases using lymphoscintigraphy. It is likely that these patients previously had some form of lymphopathy, and the lymphedema arose after the action of some external factor. However, there are no studies in the literature that adequately assess the asymptomatic contralateral limbs of these patients or address the prevalence and characteristics of the abnormal lymphatic circulation in these limbs. The aim of this study was to assess the prevalence of abnormal lymphatic circulation in the asymptomatic contralateral limbs of patients with unilateral lymphedema of the LL. METHODS: Forty-three patients from the angiology and vascular surgery ward of the Hospital das Clínicas da Universidade Federal de Pernambuco with unilateral lymphedema of the LL underwent lymphoscintigraphy. All patients received a subcutaneous injection of 0.2 mL (74 MBq) of a solution of dextran 70 labeled with technetium Tc 99m in the first interdigital space of each foot. Images were obtained on two occasions: 10 minutes and 1 hour after the injection. The study design was transversal prospective. RESULTS: Among the 43 asymptomatic LLs, 30 (70%) showed abnormal lymphatic circulation. The lymphoscintigraphic abnormalities found were the following: reduced visualization of the lymphatic vessels and lymph nodes during 1 hour (83%), collateral circulation (30%), visualization of the inguinal and pelvic lymph nodes after at least 1 hour (30%), visualization of the popliteal lymph nodes (20%), dilation and lymphatic tortuosity (20%), and dermal backflow (10%). CONCLUSIONS: In this study,70% of the patients with unilateral lymphedema of the LL had some form of lymphopathy in the contralateral limb during the lymphoscintigraphic examination. These findings could favor the early treatment of these patients to prevent the disease from progressing to its most severe stage.


Assuntos
Anormalidades Linfáticas/diagnóstico por imagem , Linfedema/diagnóstico por imagem , Adolescente , Adulto , Idoso , Dextranos , Feminino , Humanos , Perna (Membro)/irrigação sanguínea , Anormalidades Linfáticas/complicações , Linfedema/complicações , Linfocintigrafia/métodos , Masculino , Pessoa de Meia-Idade , Compostos de Organotecnécio , Estudos Prospectivos , Compostos Radiofarmacêuticos , Adulto Jovem
11.
Int. braz. j. urol ; 43(2): 335-344, Mar.-Apr. 2017. tab, graf
Artigo em Inglês | LILACS-Express | ID: biblio-840821

RESUMO

ABSTRACT Objective To validate the application of the bacterial cellulose (BC) membrane as a protecting barrier to the urethra. Materials and Methods Forty female Wistar rats (four groups of 10): Group 1 (sham), the urethra was dissected as in previous groups and nothing applied around; Group 2, received a 0.7cm strip of the BC applied around the urethra just below the bladder neck; Group 3, received a silicon strip with the same dimensions as in group 2; Group 4, had a combination of 2 and 3 groups being the silicon strip applied over the cellulosic material. Half of the animals in each group were killed at 4 and 8 months. Bladder and urethra were fixed in formalin for histological analysis. Results Inflammatory infiltrates were more intense at 4 months at lymphonodes (80% Grade 2), statistically different in the group 2 compared with groups 1 (p=0.0044) and 3 (p=0.0154). At 8 months, all samples were classified as grade 1 indicating a less intense inflammatory reaction in all groups. In group 2, at 8 months, there was a reduction in epithelial thickness (30±1μm) when com-pared to groups 1 (p=0.0001) and 3 (p<0.0001). Angiogenesis was present in groups 2 and 4 and absent in group 3. In BC implant, at 4 and 8 months, it was significant when comparing groups 4 with 1 (p=0.0159). Conclusion BC membrane was well integrated to the urethral wall promoting tissue remodeling and strengthening based on morphometric and histological results and may be a future option to prevent urethral damage.

12.
J Mater Sci Mater Med ; 28(3): 37, 2017 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-28144849

RESUMO

Duraplasty is necessary in nearly 30% of all neurological surgeries. Different tissues and materials have been evaluated in dura mater repair or as dural substitutes in neurosurgery. The aim was to evaluate the biocompatibility of the bacterial cellulose (BC) membranes, produced from sugarcane molasses, for dural defect repair in rats. Forty adults males Wistar rats divided into two groups: a control (ePTFE) and an experimental (BC). Bilateral frontoparietal craniectomy was performed, and a dural defect was created. The arachnoid underlying defect was disrupted with a narrow hook. The animals were observed for 120 days. There were no cases of infection, cerebrospinal fluid fistulae, delayed hemorrhages, behavior disturbances, seizures and palsies. The BC membrane showed to have suitable biocompatibility properties, was not induced immune reaction, nor chronic inflammatory response and absence of neurotoxicity signals.


Assuntos
Bactérias/química , Materiais Biocompatíveis/química , Celulose/química , Dura-Máter/efeitos dos fármacos , Animais , Dura-Máter/lesões , Sistema Imunitário , Inflamação , Masculino , Melaço , Ratos , Ratos Wistar , Saccharum , Cicatrização
13.
Rev. Col. Bras. Cir ; 44(1): 72-80, Jan.-Feb. 2017. tab, graf
Artigo em Inglês | LILACS-Express | ID: biblio-842637

RESUMO

ABSTRACT Objective: to evaluate the efficacy of Bacterial Cellulose (BC) membrane dressings in the treatment of lower limb venous ulcers. Methods: we carried out a prospective, randomized, controlled study of 25 patients with chronic venous ulcer disease in the lower limbs from the Angiology and Vascular Surgery Service of the Federal University of Pernambuco Hospital and from the Salgado Polyclinic of the County Health Department, Caruaru, Pernambuco. We randomly assigned patients to two groups: control group, receiving dressings with triglyceride oil (11 patients) and experimental group, treated with BC membrane (14 patients). We followed the patients for a period of 120 days. Results: There was a reduction in the wound area in both groups. There were no infections or reactions to the product in any of the groups. Patients in the BC group showed decreased pain and earlier discontinuation of analgesic use. Conclusion: BC membrane can be used as a dressing for the treatment of varicose ulcers of the lower limbs.


RESUMO Objetivo: avaliar a eficácia de curativos com membrana de Celulose Bacteriana (CB) no tratamento de úlceras venosas de membros inferiores. Métodos: estudo prospectivo, randomizado e controlado de 25 pacientes com úlceras decorrentes de doença venosa crônica nos membros inferiores provenientes do Serviço de Angiologia e Cirurgia Vascular do Hospital de Clínicas da Universidade Federal de Pernambuco e da Policlínica do Salgado da Secretaria Municipal de Saúde, Caruaru, Pernambuco. Os pacientes foram distribuídos aleatoriamente em dois grupos: grupo controle, que recebeu curativos com óleo de triglicerídeos (11 pacientes) e grupo experimental, tratado com membrana de CB (14 pacientes). Os pacientes foram acompanhados por um período de 120 dias. Resultados: houve uma redução na área de ferida em ambos os grupos. Não houve infecção ou reações ao produto em nenhum dos grupos. Pacientes do grupo CB mostraram diminuição da dor e interrupção mais precoce do uso de analgésicos. Conclusão: a membrana de CB pode ser usada como curativo para o tratamento de úlceras varicosas dos membros inferiores.

14.
Int Braz J Urol ; 43(2): 335-344, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-27819753

RESUMO

OBJECTIVE: To validate the application of the bacterial cellulose (BC) membrane as a protecting bar-rier to the urethra. MATERIALS AND METHODS: Forty female Wistar rats (four groups of 10): Group 1 (sham), the urethra was dissected as in previous groups and nothing applied around; Group 2, received a 0.7cm strip of the BC applied around the urethra just below the bladder neck; Group 3, received a silicon strip with the same dimensions as in group 2; Group 4, had a combination of 2 and 3 groups being the silicon strip applied over the cellulosic material. Half of the animals in each group were killed at 4 and 8 months. Bladder and urethra were fixed in formalin for histological analysis. RESULTS: Inflammatory infiltrates were more intense at 4 months at lymphonodes (80% Grade 2), statistically different in the group 2 compared with groups 1 (p=0.0044) and 3 (p=0.0154). At 8 months, all samples were classified as grade 1 indicating a less intense inflammatory reaction in all groups. In group 2, at 8 months, there was a reduction in epithelial thickness (30±1µm) when com-pared to groups 1 (p=0.0001) and 3 (p<0.0001). Angiogenesis was present in groups 2 and 4 and absent in group 3. In BC implant, at 4 and 8 months, it was significant when comparing groups 4 with 1 (p=0.0159). CONCLUSION: BC membrane was well integrated to the urethral wall promoting tissue remodeling and strengthening based on morphometric and histological results and may be a future option to prevent urethral damage.


Assuntos
Bactérias/química , Materiais Biocompatíveis/farmacologia , Celulose/farmacologia , Implantação de Prótese/efeitos adversos , Uretra/lesões , Doenças Uretrais/prevenção & controle , Esfíncter Urinário Artificial/efeitos adversos , Animais , Feminino , Membranas , Modelos Animais , Ratos Wistar , Reprodutibilidade dos Testes , Silicones/farmacologia , Fatores de Tempo , Resultado do Tratamento , Uretra/patologia , Doenças Uretrais/patologia , Incontinência Urinária/cirurgia
15.
Int. braz. j. urol ; 42(6): 1220-1227, Nov.-Dec. 2016. tab, graf
Artigo em Inglês | LILACS-Express | ID: biblio-828940

RESUMO

ABSTRACT Purpose: This study was to confirm the safety and efficacy of BC dressing when used in surgical male wound healing at the urogenital area. Methods: Open, non-controlled clinical study of phase II. A total of 141 patients, among those children, adolescents and adults with hypospadias (112), epispadias (04), phymosis (13) and Peyronie's disease (12) that had a BC dressing applied over the operated area after surgery. A written informed consent was obtained from all participants. Study exclusion criteria were patients with other alternative treatment indications due to the severity, extent of the injury or the underlying disease. The outcomes evaluated were efficacy, safe and complete healing. The costs were discussed. Results: In 68% patients, the BC dressing fell off spontaneously. The BC was removed without complications in 13% of patients at the outpatient clinic during the follow-up visit and 17% not reported the time of removal. In 3% of the cases, the dressing fell off early. Complete healing was observed between 8th and 10th days after surgery. The BC dressings have shown a good tolerance by all the patients and there were no reports of serious adverse events. Conclusion: The bacterial cellulose dressings have shown efficacy, safety and that can be considered as a satisfactory alternative for postoperative wound healing in urogenital area and with low cost.

16.
Acta Cir Bras ; 31(10): 650-654, 2016 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-27828597

RESUMO

PURPOSE: : To investigate the protective effect of L-arginine on the prostate (nonneoplasic) of rats with radiation-induced injury. METHODS: : Twenty-nine Wistar rats, male adult, allocated into three groups: Control group (C) was not exposed to irradiation (n=10); Radiated group (R) had undergone pelvic irradiation (n=10); Supplemented and radiated group (R+S) had undergone pelvic irradiation plus L-arginine supplementation (n=9). The animals were observed for signs of toxicity. After euthanization, the prostate was dissected under magnification and stained by hematoxylin and eosin to study acinar structures and stained with Picrosirius red for collagen analysis. RESULTS: : After radiation exposure, all animals presented diarrhea, but supplementation with L-arginine reduced this effect. The weight gain in the R+S group was significantly higher than in the C and R groups. In the R+S group the collagen density and the prostate acinar area was similar to the R and C groups. Epithelial height was significantly reduced in group R compared with group C (p<0.0001). When comparing the group R+S with R, a statistical difference was observed to be present (p<0.0001). CONCLUSIONS: : Pelvic radiation promotes systemic effects and some structural modifications in the ventral prostate of rats. These modifications can be prevented by oral supplementation with L-arginine.


Assuntos
Arginina/farmacologia , Suplementos Nutricionais , Próstata/efeitos dos fármacos , Próstata/efeitos da radiação , Lesões por Radiação/prevenção & controle , Protetores contra Radiação/farmacologia , Animais , Peso Corporal , Colágeno/análise , Masculino , Óxido Nítrico/metabolismo , Pelve/efeitos da radiação , Próstata/patologia , Distribuição Aleatória , Ratos Wistar , Reprodutibilidade dos Testes , Resultado do Tratamento
17.
Acta cir. bras ; 31(10): 650-654, Oct. 2016. tab, graf
Artigo em Inglês | LILACS | ID: biblio-827653

RESUMO

ABSTRACT PURPOSE: To investigate the protective effect of L-arginine on the prostate (nonneoplasic) of rats with radiation-induced injury. METHODS: Twenty-nine Wistar rats, male adult, allocated into three groups: Control group (C) was not exposed to irradiation (n=10); Radiated group (R) had undergone pelvic irradiation (n=10); Supplemented and radiated group (R+S) had undergone pelvic irradiation plus L-arginine supplementation (n=9). The animals were observed for signs of toxicity. After euthanization, the prostate was dissected under magnification and stained by hematoxylin and eosin to study acinar structures and stained with Picrosirius red for collagen analysis. RESULTS: After radiation exposure, all animals presented diarrhea, but supplementation with L-arginine reduced this effect. The weight gain in the R+S group was significantly higher than in the C and R groups. In the R+S group the collagen density and the prostate acinar area was similar to the R and C groups. Epithelial height was significantly reduced in group R compared with group C (p<0.0001). When comparing the group R+S with R, a statistical difference was observed to be present (p<0.0001). CONCLUSIONS: Pelvic radiation promotes systemic effects and some structural modifications in the ventral prostate of rats. These modifications can be prevented by oral supplementation with L-arginine.


Assuntos
Animais , Masculino , Arginina/farmacologia , Próstata/efeitos dos fármacos , Próstata/efeitos da radiação , Lesões por Radiação/prevenção & controle , Protetores contra Radiação/farmacologia , Suplementos Nutricionais , Pelve/efeitos da radiação , Próstata/patologia , Peso Corporal , Distribuição Aleatória , Reprodutibilidade dos Testes , Colágeno/análise , Resultado do Tratamento , Ratos Wistar , Óxido Nítrico/metabolismo
18.
Int Braz J Urol ; 42(6): 1220-1227, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-27649111

RESUMO

PURPOSE: This study was to confirm the safety and efficacy of BC dressing when used in surgical male wound healing at the urogenital area. METHODS: Open, non-controlled clinical study of phase II. A total of 141 patients, among those children, adolescents and adults with hypospadias (112), epispadias (04), phymosis (13) and Peyronie's disease (12) that had a BC dressing applied over the operated area after surgery. A written informed consent was obtained from all participants. Study exclusion criteria were patients with other alternative treatment indications due to the severity, extent of the injury or the underlying disease. The outcomes evaluated were efficacy, safe and complete healing. The costs were discussed. RESULTS: In 68% patients, the BC dressing fell off spontaneously. The BC was removed without complications in 13% of patients at the outpatient clinic during the follow-up visit and 17% not reported the time of removal. In 3% of the cases, the dressing fell off early. Complete healing was observed between 8th and 10th days after surgery. The BC dressings have shown a good tolerance by all the patients and there were no reports of serious adverse events. CONCLUSION: The bacterial cellulose dressings have shown efficacy, safety and that can be considered as a satisfactory alternative for postoperative wound healing in urogenital area and with low cost.


Assuntos
Bandagens , Doenças do Pênis/cirurgia , Pênis/cirurgia , Poliuretanos/uso terapêutico , Anormalidades Urogenitais/cirurgia , Cicatrização , Adolescente , Criança , Pré-Escolar , Epispadia/cirurgia , Humanos , Hipospadia/cirurgia , Masculino , Pessoa de Meia-Idade , Induração Peniana/cirurgia , Fimose/cirurgia , Período Pós-Operatório , Resultado do Tratamento , Técnicas de Fechamento de Ferimentos
19.
J Mater Sci Mater Med ; 27(8): 129, 2016 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-27379627

RESUMO

The use of meshes for treatment of hernias continues to draw attention of surgeons and the industry in the search of an ideal prosthesis. The purpose of this work is to use meshes manufactured from bacterial cellulose, evaluate their organic tissue interaction and compare with an expanded polytetrafluorethylene (ePTFE's) prosthesis used to repair acute defect of muscle aponeurotic induced in rats. Forty-five male Wistar rats were classified using the following criteria: (1) surgical repair of acute muscle aponeurotic defect with perforated bacterial cellulose film (PBC; n = 18); (2) compact bacterial cellulose film (CBC; n = 12) and (3) ePTFE; (n = 15). After postoperative period, rectangles (2 × 3 cm) including prosthesis, muscles and peritoneum were collected for biomechanical, histological and stereological analysis. In all cases, the maximum acceptable error probability for rejecting the null hypothesis was 5 %. Between PBC and CBC samples, the variables of strain (P = 0.011) and elasticity (P = 0.035) were statistically different. The same was found between CBC and ePTFE (elasticity, P = 0.000; strain, P = 0.009). PBC differed from CBC for giant cells (P = 0.001) and new blood vessels (P = 0.000). In conclusion, there was biological integration and biomechanical elasticity of PBC; therefore, we think this option should be considered as a new alternative biomaterial for use as a bio prosthesis.


Assuntos
Músculos Abdominais/patologia , Celulose/química , Hérnia/terapia , Politetrafluoretileno/química , Telas Cirúrgicas , Aderências Teciduais/prevenção & controle , Animais , Bactérias/química , Materiais Biocompatíveis/química , Hidrogéis/química , Inflamação , Masculino , Peritônio/patologia , Ratos , Ratos Wistar , Estresse Mecânico
20.
Braz. j. otorhinolaryngol. (Impr.) ; 82(2): 203-208, Mar.-Apr. 2016. tab, graf
Artigo em Inglês | LILACS | ID: lil-780978

RESUMO

ABSTRACT INTRODUCTION: Promising treatments for tympanic membrane perforation closure have been studied. Therapies derived from tissue engineering probably eliminate the need for conventional surgery. Bacterial cellulose is presented as an alternative that is safe, biocompatible, and has low toxicity. OBJECTIVES: To investigate the effect on healing of direct application of a bacterial cellulose graft on the tympanic membrane compared to the conventional approach with autologous fascia. METHODS: Randomized controlled trial. Forty patients with tympanic membrane perforations secondary to chronic otitis media were included, and were randomly assigned to an experimental group (20), treated with a bacterial cellulose graft (BC) and control group (20), treated with autologous temporal fascia (fascia). We evaluated the surgical time, hospital stay, time of epithelialization and the rate of tympanic perforation closure. Hospital costs were compared. The statistical significance level accepted was established at p < 0.05. RESULTS: The closure of perforations was similar in both groups. The average operation time in the fascia group was 76.50 min versus 14.06 min bacterial cellulose in the group (p = 0.0001). The hospital cost by the Brazilian public health system was R$ 600.00 for the bacterial cellulose group, and R$ 7778.00 for the fascia group (p = 0.0001). CONCLUSION: Bacterial cellulose grafts promoted the closure of the tympanic membrane perforations, and were demonstrated to be innovative, effective, safe, minimally invasive, efficacious and to have a very low cost.


RESUMO INTRODUÇÃO: Tratamentos promissores para o fechamento da perfuração da membrana timpânica vêm sendo estudados. Terapias provenientes de engenharia de tecidos provavelmente eliminarão a necessidade de uma intervenção cirúrgica convencional. A celulose bacteriana apresenta-se como uma alternativa por ser segura, de baixa toxicidade, biocompatível. OBJETIVOS: Investigar o efeito da aplicação direta do enxerto da celulose bacteriana na cicatrização de perfurações da membrana timpânica, comparado ao procedimento convencional com fáscia autóloga. MÉTODO: Incluíram-se 40 pacientes com perfuração da membrana timpânica por otite média crônica simples. Randomizados de 1 a 40, onde os ímpares (20) foram tratados com enxerto de celulose bacteriana (CB), e os pares (20), com enxerto de fáscia temporal autóloga (fáscia). Estudo clínico controlado e randomizado. O tempo cirúrgico e de hospitalização foram o tempo de epitelização e custos hospitalares. RESULTADOS: O fechamento das perfurações foi semelhante nos dois grupos. O tempo médio da cirurgia no grupo fáscia foi de 76,50 minutos e de 14,06 minutos no grupo com celulose bacteriana (p = 0,0001). O custo hospitalar pela tabela do SUS foi de R$ 600,00 para o grupo CB e R$ 7.778,00 para o grupo fáscia (p = 0,0001). CONCLUSÃO: A celulose bacteriana promoveu o fechamento da perfuração do tímpano, mostrando-se inovador, seguro, eficaz, efetivo, minimamente invasivo e de baixo custo.


Assuntos
Adulto , Feminino , Humanos , Masculino , Celulose/administração & dosagem , Perfuração da Membrana Timpânica/cirurgia , Bioprótese , Fáscia/transplante , Fatores de Tempo , Resultado do Tratamento , Cicatrização/fisiologia
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