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1.
Nat Prod Res ; 33(16): 2383-2388, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-29475383

RESUMO

This study reported the antimicrobial activity of the bark extract of Davilla nitida on multidrug resistant bacteria isolated from Diabetic Foot Infections. Antibacterial activity of the bark extract was evaluated by agar Disk-Diffusion (DD), Broth Dilution (BD), Checkerboard and Time-kill methods. The extract showed a significant antibacterial activity against all groups of bacteria tested. BD was more sensitive for determining the antibacterial activity of the bark extract than the DD method. The bark extract inhibited the growth of bacteria with high-levels of antibiotic-resistance, such as Pseudomonas spp. (100.0%), Enterobacer spp. (88.89%), Staphylococcus aureus (54.55%), Streptococcus pneumoniae (75.0%), Staphylococcus saprophyticus (92.86%). The combination of extract with antibiotics resulted in an additive effect against most of the strains tested. Time-kill kinetics profiles of bark extract showed bactericidal and time-dependent properties. Our results suggest that the bark extract of Davilla nitida is a source of bioactive compounds, which may be useful against antibiotic-resistant bacteria.


Assuntos
Antibacterianos/farmacologia , Pé Diabético/complicações , Farmacorresistência Bacteriana Múltipla/efeitos dos fármacos , Extratos Vegetais/farmacologia , Antibacterianos/isolamento & purificação , Bactérias/efeitos dos fármacos , Pé Diabético/microbiologia , Sinergismo Farmacológico , Etanol , Humanos , Cinética , Testes de Sensibilidade Microbiana , Casca de Planta/química , Infecções Estafilocócicas/tratamento farmacológico
2.
Front Microbiol ; 9: 3198, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-30723463

RESUMO

Klebsiella pneumoniae is an important opportunistic pathogen that commonly causes nosocomial infections and contributes to substantial morbidity and mortality. We sought to investigate the antibiotic resistance profile, pathogenic potential and the clonal relationships between K. pneumoniae (n = 25) isolated from patients and sources at a tertiary care hospital's intensive care units (ICUs) in the northern region of Brazil. Most of K. pneumoniae isolates (n = 21, 84%) were classified as multidrug resistant (MDR) with high-level resistance to ß-lactams, aminoglycosides, quinolones, tigecycline, and colistin. All the 25 isolates presented extended-spectrum beta-lactamase-producing (ESBL), including carbapenemase producers, and carried the bla KPC (100%), bla TEM (100%), bla SHV variants (n = 24, 96%), bla OXA-1 group (n = 21, 84%) and bla CTX-M-1 group (n = 18, 72%) genes. The K2 serotype was found in 4% (n = 1) of the isolates, and the K1 was not detected. The virulence-associated genes found among the 25 isolates were mrkD (n = 24, 96%), fimH-1 (n = 22, 88%), entB (100%), iutA (n = 10, 40%), ybtS (n = 15, 60%). The genes related with efflux pumps and outer membrane porins found were AcrAB (100%), tolC (n = 24, 96%), mdtK (n = 22, 88%), OmpK35 (n = 15, 60%), and OmpK36 (n = 7, 28%). ERIC-PCR was employed to determine the clonal relationship between the different isolated strains. The obtained ERIC-PCR patterns revealed that the similarity between isolates was above 70%. To determine the sequence types (STs) a multilocus sequence typing (MLST) assay was used. The results indicated the presence of high-risk international clones among the isolates. In our study, the wide variety of MDR K. pneumoniae harboring ß-lactams and virulence genes strongly suggest a necessity for the implementation of effective strategies to prevent and control the spread of antibiotic resistant infections.

3.
Rev Soc Bras Med Trop ; 50(1): 67-74, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-28327804

RESUMO

INTRODUCTION: Despite their high toxicity, antimonials and amphotericin B deoxycholate are commonly used for treating visceral leishmaniasis (VL). Few studies showing conflictive data about their efficacy and adverse events in pediatric population are available. This study aimed to evaluate efficacy and safety of amphotericin B deoxycholate vs. that of N-methylglucamine antimoniate in treating pediatric VL in Brazil. METHODS: This was a randomized, open-label, 2-arm and controlled pilot clinical trial. Treatment naïve children and adolescents with VL without signs of severe illness were treated with N-methylglucamine antimoniate (20mg/kg/day for 20 days) or amphotericin B deoxycholate (1 mg/kg/day for 14 days). All patients were diagnosed with positive direct examination and/or positive PCR for Leishmania spp. performed in bone marrow samples. The primary efficacy end-point was VL cure determined after 180 days of completion of treatment. The analysis was performed using intention-to-treat (ITT) and per protocol (PP) analyses. RESULTS: In total, 101 volunteers were assessed. Efficacy was similar for both groups. The antimonial (n=51) and amphotericin B groups (n=50) had a cure rate of 94.1% and 100%, and 94% and 97.9% according to ITT and PP analyses, respectively. All patients reported adverse events (AE). Serious AE incidence was similar in both groups. Five individuals were excluded from the study because of severe adverse events. CONCLUSIONS: N-methylglucamine antimoniate and amphotericin B deoxycholate have similar efficacy and adverse events rate in pediatric patients with VL.


Assuntos
Anfotericina B/uso terapêutico , Antiprotozoários/uso terapêutico , Ácido Desoxicólico/uso terapêutico , Leishmaniose Visceral/tratamento farmacológico , Meglumina/uso terapêutico , Compostos Organometálicos/uso terapêutico , Anfotericina B/efeitos adversos , Antiprotozoários/efeitos adversos , Criança , Pré-Escolar , Ácido Desoxicólico/efeitos adversos , Combinação de Medicamentos , Feminino , Humanos , Masculino , Meglumina/efeitos adversos , Antimoniato de Meglumina , Compostos Organometálicos/efeitos adversos , Projetos Piloto , Resultado do Tratamento
4.
Nat Prod Res ; 31(24): 2931-2935, 2017 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-28326851

RESUMO

Brosimum gaudichaudii Trécul., a plant that belongs to Moraceae family, is found throughout the Brazilian Cerrado. The antimicrobial activities of ethanolic bark and leaf extracts of B. gaudichaudii were tested against multiresistant bacteria isolated from diabetic foot infections (DFIs). Antimicrobial activity of the extracts was evaluated by agar disc diffusion (DD) and broth dilution (BD) methods. By BD method, bark (53.85, 45.83%) and leaf (42.31, 50.00%) extracts contained antimicrobial activity against both gram-negative and gram-positive bacteria. Increased antimicrobial activity was observed when bark and leaf extracts were tested against Staphylococcus aureus (63.64%) and Pseudomonas aeruginosa (66.67%). Statistical analyses of bark and leaf extract demonstrated antimicrobial activity against both gram-positive (p = 0.000) and gram-negative bacteria (p = 0.012). Extract of bark (p = 0.075) or leaf (p = 0.005) associated with ACA antibiotic showed antimicrobial activity against gram-positive bacteria. Our study suggests that the bark and leaf extracts contain bioactive compounds with antimicrobial activity against multidrug resistant strains.


Assuntos
Antibacterianos/isolamento & purificação , Moraceae/química , Antibacterianos/química , Antibacterianos/farmacologia , Brasil , Resistência a Múltiplos Medicamentos/efeitos dos fármacos , Bactérias Gram-Negativas/efeitos dos fármacos , Bactérias Gram-Positivas/efeitos dos fármacos , Casca de Planta/química , Extratos Vegetais/isolamento & purificação , Extratos Vegetais/farmacologia , Folhas de Planta/química
5.
Rev. Soc. Bras. Med. Trop ; 50(1): 67-74, Jan.-Feb. 2017. tab, graf
Artigo em Inglês | LILACS-Express | ID: biblio-842815

RESUMO

ABSTRACT INTRODUCTION Despite their high toxicity, antimonials and amphotericin B deoxycholate are commonly used for treating visceral leishmaniasis (VL). Few studies showing conflictive data about their efficacy and adverse events in pediatric population are available. This study aimed to evaluate efficacy and safety of amphotericin B deoxycholate vs. that of N-methylglucamine antimoniate in treating pediatric VL in Brazil. METHODS This was a randomized, open-label, 2-arm and controlled pilot clinical trial. Treatment naïve children and adolescents with VL without signs of severe illness were treated with N-methylglucamine antimoniate (20mg/kg/day for 20 days) or amphotericin B deoxycholate (1 mg/kg/day for 14 days). All patients were diagnosed with positive direct examination and/or positive PCR for Leishmania spp. performed in bone marrow samples. The primary efficacy end-point was VL cure determined after 180 days of completion of treatment. The analysis was performed using intention-to-treat (ITT) and per protocol (PP) analyses. RESULTS In total, 101 volunteers were assessed. Efficacy was similar for both groups. The antimonial (n=51) and amphotericin B groups (n=50) had a cure rate of 94.1% and 100%, and 94% and 97.9% according to ITT and PP analyses, respectively. All patients reported adverse events (AE). Serious AE incidence was similar in both groups. Five individuals were excluded from the study because of severe adverse events. CONCLUSIONS N-methylglucamine antimoniate and amphotericin B deoxycholate have similar efficacy and adverse events rate in pediatric patients with VL.

6.
BMC Immunol ; 17(1): 22, 2016 07 04.
Artigo em Inglês | MEDLINE | ID: mdl-27377926

RESUMO

BACKGROUND: Mast cells are hematopoietically derived cells that play a role in inflammatory processes such as allergy, as well as in the immune response against pathogens by the selective and rapid release of preformed and lipid mediators, and the delayed release of cytokines. The native homotetrameric lectin ArtinM, a D-mannose binding lectin purified from Artocarpus heterophyllus seeds, is one of several lectins that are able to activate mast cells. Besides activating mast cells, ArtinM has been shown to affect several biological responses, including immunomodulation and acceleration of wound healing. Because of the potential pharmacological application of ArtinM, a recombinant ArtinM (rArtinM) was produced in Escherichia coli. The current study evaluated the ability of rArtinM to induce mast cell degranulation and activation. RESULTS: The glycan binding specificity of rArtinM was similar to that of jArtinM. rArtinM, via its CRD, was able to degranulate, releasing ß-hexosaminidase and TNF-α, and to promote morphological changes on the mast cell surface. Moreover, rArtinM induced the release of the newly-synthesized mediator, IL-4. rArtinM does not have a co-stimulatory effect on the FcεRI degranulation via. The IgE-dependent mast cell activation triggered by rArtinM seems to be dependent on NFkB activation. CONCLUSIONS: The lectin rArtinM has the ability to activate and degranulate mast cells via their CRDs. The present study indicates that rArtinM is a suitable substitute for the native form, jArtinM, and that rArtinM may serve as an important and reliable pharmacological agent.


Assuntos
Mastócitos/imunologia , Lectinas de Plantas/imunologia , Proteínas Recombinantes/imunologia , Animais , Artocarpus/imunologia , Degranulação Celular , Linhagem Celular , Clonagem Molecular , Escherichia coli/genética , Histamina/metabolismo , Imunoglobulina E/metabolismo , Imunomodulação , Interleucina-4/metabolismo , Manose/metabolismo , NF-kappa B/metabolismo , Lectinas de Plantas/isolamento & purificação , Ligação Proteica , Ratos , Proteínas Recombinantes/isolamento & purificação , beta-N-Acetil-Hexosaminidases/metabolismo
7.
Rev Soc Bras Med Trop ; 48(5): 546-54, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-26516963

RESUMO

INTRODUCTION: This study aimed to determine the frequencies of bacterial isolates cultured from diabetic foot infections and assess their resistance and susceptibility to commonly used antibiotics. METHODS: This prospective study included 41 patients with diabetic foot lesions. Bacteria were isolated from foot lesions, and their antibiotic susceptibility pattern was determined using the Kirby-Bauer disk diffusion method and/or broth method [minimum inhibitory concentration (MIC)]. RESULTS: The most common location of ulceration was the toe (54%), followed by the plantar surface (27%) and dorsal portion (19%). A total of 89 bacterial isolates were obtained from 30 patients. The infections were predominantly due to Gram-positive bacteria and polymicrobial bacteremia. The most commonly isolated Gram-positive bacteria were Staphylococcus aureus, followed by Staphylococcus saprophyticus, Staphylococcus epidermidis, Streptococcus agalactiae, and Streptococcus pneumoniae. The most commonly isolated Gram-negative bacteria were Proteus spp. and Enterobacterspp., followed by Escherichia coli, Pseudomonasspp., and Citrobacterspp. Nine cases of methicillin-resistant Staphylococcus aureus (MRSA) had cefoxitin resistance, and among these MRSA isolates, 3 were resistant to vancomycin with the MIC technique. The antibiotic imipenem was the most effective against both Gram-positive and Gram-negative bacteria, and gentamicin was effective against Gram-negative bacteria. CONCLUSIONS: The present study confirmed the high prevalence of multidrug-resistant pathogens in diabetic foot ulcers. It is necessary to evaluate the different microorganisms infecting the wound and to know the antibiotic susceptibility patterns of the isolates from the infected wound. This knowledge is crucial for planning treatment with the appropriate antibiotics, reducing resistance patterns, and minimizing healthcare costs.


Assuntos
Pé Diabético/microbiologia , Farmacorresistência Bacteriana/efeitos dos fármacos , Bactérias Gram-Negativas/efeitos dos fármacos , Bactérias Gram-Positivas/efeitos dos fármacos , Adulto , Idoso , Antibacterianos/farmacologia , Feminino , Bactérias Gram-Negativas/classificação , Bactérias Gram-Positivas/classificação , Humanos , Masculino , Testes de Sensibilidade Microbiana , Pessoa de Meia-Idade , Estudos Prospectivos , Adulto Jovem
8.
Rev. Soc. Bras. Med. Trop ; 48(5): 546-554, Sept.-Oct. 2015. tab
Artigo em Inglês | LILACS | ID: lil-763327

RESUMO

ABSTRACTINTRODUCTION: This study aimed to determine the frequencies of bacterial isolates cultured from diabetic foot infections and assess their resistance and susceptibility to commonly used antibiotics.METHODS: This prospective study included 41 patients with diabetic foot lesions. Bacteria were isolated from foot lesions, and their antibiotic susceptibility pattern was determined using the Kirby-Bauer disk diffusion method and/or broth method [minimum inhibitory concentration (MIC)].RESULTS: The most common location of ulceration was the toe (54%), followed by the plantar surface (27%) and dorsal portion (19%). A total of 89 bacterial isolates were obtained from 30 patients. The infections were predominantly due to Gram-positive bacteria and polymicrobial bacteremia. The most commonly isolated Gram-positive bacteria were Staphylococcus aureus, followed by Staphylococcus saprophyticus, Staphylococcus epidermidis, Streptococcus agalactiae, and Streptococcus pneumoniae. The most commonly isolated Gram-negative bacteria were Proteus spp. and Enterobacterspp., followed by Escherichia coli, Pseudomonasspp., and Citrobacterspp. Nine cases of methicillin-resistant Staphylococcus aureus (MRSA) had cefoxitin resistance, and among these MRSA isolates, 3 were resistant to vancomycin with the MIC technique. The antibiotic imipenem was the most effective against both Gram-positive and Gram-negative bacteria, and gentamicin was effective against Gram-negative bacteria.CONCLUSIONS: The present study confirmed the high prevalence of multidrug-resistant pathogens in diabetic foot ulcers. It is necessary to evaluate the different microorganisms infecting the wound and to know the antibiotic susceptibility patterns of the isolates from the infected wound. This knowledge is crucial for planning treatment with the appropriate antibiotics, reducing resistance patterns, and minimizing healthcare costs.


Assuntos
Adulto , Idoso , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Adulto Jovem , Pé Diabético/microbiologia , Farmacorresistência Bacteriana/efeitos dos fármacos , Bactérias Gram-Negativas/efeitos dos fármacos , Bactérias Gram-Positivas/efeitos dos fármacos , Antibacterianos/farmacologia , Bactérias Gram-Negativas/classificação , Bactérias Gram-Positivas/classificação , Testes de Sensibilidade Microbiana , Estudos Prospectivos
9.
Rev Soc Bras Med Trop ; 47(1): 38-46, 2014.
Artigo em Inglês | MEDLINE | ID: mdl-24603735

RESUMO

INTRODUCTION: This study aimed to describe the main features of visceral leishmaniasis (VL), both related to and independent of human immunodeficiency virus (HIV) infection, in patients who were registered in Tocantins, Brazil. METHODS: Data from 1,779 new patients with VL, 33 of whom were also infected with HIV, were reviewed. RESULTS: The incidence of VL/HIV coinfection increased from 0.32/100,000 inhabitants in 2007 to 1.08/100,000 inhabitants in 2010. VL occurred predominantly in children aged 10 years or younger, while VL/HIV was more common in patients aged between 18 and 50 years. There were more male patients in the VL/HIV group than in the VL group. Relapse rates were also considerably higher in the VL/HIV (9.1%) group than in the VL group (1.5%). Despite a similar clinical presentation, VL/HIV patients exhibited a higher proportion (24.2%) of concomitant infectious diseases and jaundice. Pentavalent antimonials were used for the initial treatment of VL and VL/HIV infections. However, amphotericin B deoxycholate and liposomal amphotericin B were also widely used in the treatment of VL/HIV coinfection. The mortality rate was higher in the VL/HIV coinfection group (19.4%) than in the VL group (5.4%). Furthermore, the mortality rate due to other causes was significantly higher in the VL/HIV group (12.9%) than in the VL group (0.7%). CONCLUSIONS: The study showed that the incidence, clinical characteristics and outcomes among the VL and VL/HIV patients in this state are similar to those from other endemic regions, indicating that both infections are emerging with increasing frequency in Brazil.


Assuntos
Infecções Oportunistas Relacionadas com a AIDS/epidemiologia , Leishmaniose Visceral/epidemiologia , Infecções Oportunistas Relacionadas com a AIDS/tratamento farmacológico , Adolescente , Adulto , Brasil/epidemiologia , Criança , Pré-Escolar , Escolaridade , Feminino , Humanos , Incidência , Lactente , Recém-Nascido , Leishmaniose Visceral/tratamento farmacológico , Masculino , Pessoa de Meia-Idade , Recidiva , Adulto Jovem
10.
Rev. Soc. Bras. Med. Trop ; 47(1): 38-46, Jan-Feb/2014. tab, graf
Artigo em Inglês | LILACS | ID: lil-703170

RESUMO

Introduction: This study aimed to describe the main features of visceral leishmaniasis (VL), both related to and independent of human immunodeficiency virus (HIV) infection, in patients who were registered in Tocantins, Brazil. Methods: Data from 1,779 new patients with VL, 33 of whom were also infected with HIV, were reviewed. Results: The incidence of VL/HIV coinfection increased from 0.32/100,000 inhabitants in 2007 to 1.08/100,000 inhabitants in 2010. VL occurred predominantly in children aged 10 years or younger, while VL/HIV was more common in patients aged between 18 and 50 years. There were more male patients in the VL/HIV group than in the VL group. Relapse rates were also considerably higher in the VL/HIV (9.1%) group than in the VL group (1.5%). Despite a similar clinical presentation, VL/HIV patients exhibited a higher proportion (24.2%) of concomitant infectious diseases and jaundice. Pentavalent antimonials were used for the initial treatment of VL and VL/HIV infections. However, amphotericin B deoxycholate and liposomal amphotericin B were also widely used in the treatment of VL/HIV coinfection. The mortality rate was higher in the VL/HIV coinfection group (19.4%) than in the VL group (5.4%). Furthermore, the mortality rate due to other causes was significantly higher in the VL/HIV group (12.9%) than in the VL group (0.7%). Conclusions: The study showed that the incidence, clinical characteristics and outcomes among the VL and VL/HIV patients in this state are similar to those from other endemic regions, indicating that both infections are emerging with increasing frequency in Brazil. .


Assuntos
Adolescente , Adulto , Criança , Pré-Escolar , Feminino , Humanos , Lactente , Recém-Nascido , Masculino , Pessoa de Meia-Idade , Adulto Jovem , Infecções Oportunistas Relacionadas com a AIDS/epidemiologia , Leishmaniose Visceral/epidemiologia , Infecções Oportunistas Relacionadas com a AIDS/tratamento farmacológico , Brasil/epidemiologia , Escolaridade , Incidência , Leishmaniose Visceral/tratamento farmacológico , Recidiva
11.
Bioengineered ; 4(5): 305-12, 2013.
Artigo em Inglês | MEDLINE | ID: mdl-23644447

RESUMO

Cancer is one of the most important health problems because many cases are difficult to prevent. Cancer still has unknown mechanisms of pathogenesis, and its capacity to produce temporary or permanent damage, besides death, is very high. Although many anticancer therapies are available, finding a cure for cancer continues to be a difficult task. Thus, many efforts have been made to develop more effective treatments, such as immunotherapy based on a new class of tumor-specific products that are produced using recombinant DNA technology. These recombinant products are used with the main objectives of killing the tumor and stimulating immune cells to respond to the cancer cells. The principal recombinant products in anticancer therapy are immunostimulants, vaccines, antibodies, immunotoxins and fusion proteins. This review focuses on the general aspects of these genetically engineered products, their clinical performance, current advances and future prospects for this type of anticancer therapy.


Assuntos
Bioengenharia/métodos , Imunoterapia/métodos , Neoplasias/tratamento farmacológico , Adjuvantes Imunológicos/biossíntese , Adjuvantes Imunológicos/uso terapêutico , Anticorpos/química , Anticorpos/uso terapêutico , Toxinas Bacterianas/biossíntese , Toxinas Bacterianas/uso terapêutico , Vacinas Anticâncer/imunologia , Vacinas Anticâncer/uso terapêutico , Humanos , Imunoconjugados/química , Imunoconjugados/uso terapêutico , Imunotoxinas/química , Imunotoxinas/uso terapêutico , Neoplasias/imunologia , Neoplasias/prevenção & controle , Proteínas Recombinantes de Fusão/biossíntese , Proteínas Recombinantes de Fusão/uso terapêutico
12.
Braz. j. infect. dis ; 17(2): 263-266, Mar.-Apr. 2013. ilus, tab
Artigo em Inglês | LILACS | ID: lil-673207

RESUMO

Visceral leishmaniasis is a relevant public health problem worldwide. Most of the reported cases in Latin America are from Brazil. Herein we report two human cases of congenitally transmitted visceral leishmaniasis in two patients who developed symptoms during pregnancy. The diagnosis was made by visual examination of Leishmania parasites in bone marrow aspirates of the mothers and by detecting parasite kDNA in bone marrow samples of the newborn children using polymerase chain reaction.


Assuntos
Feminino , Humanos , Recém-Nascido , Masculino , Gravidez , Adulto Jovem , Leishmaniose Visceral/congênito , Complicações Parasitárias na Gravidez/parasitologia , Leishmaniose Visceral/diagnóstico
13.
Braz J Infect Dis ; 17(2): 263-6, 2013.
Artigo em Inglês | MEDLINE | ID: mdl-23453409

RESUMO

Visceral leishmaniasis is a relevant public health problem worldwide. Most of the reported cases in Latin America are from Brazil. Herein we report two human cases of congenitally transmitted visceral leishmaniasis in two patients who developed symptoms during pregnancy. The diagnosis was made by visual examination of Leishmania parasites in bone marrow aspirates of the mothers and by detecting parasite kDNA in bone marrow samples of the newborn children using polymerase chain reaction.


Assuntos
Leishmaniose Visceral/congênito , Complicações Parasitárias na Gravidez/parasitologia , Feminino , Humanos , Recém-Nascido , Leishmaniose Visceral/diagnóstico , Masculino , Gravidez , Adulto Jovem
14.
BMC Pharmacol Toxicol ; 14: 5, 2013 Jan 08.
Artigo em Inglês | MEDLINE | ID: mdl-23298396

RESUMO

BACKGROUND: Adverse drug reactions (ADRs) are recognised as a common cause of hospital admissions, and they constitute a significant economic burden for hospitals. Hospital-based ADR monitoring and reporting programmes aim to identify and quantify the risks associated with the use of drugs provided in a hospital setting. This information may be useful for identifying and minimising preventable ADRs and may enhance the ability of prescribers to manage ADRs more effectively. The main objectives of this study were to evaluate ADRs that occurred during inpatient stays at the Hospital Geral de Palmas (HGP) in Tocantins, Brazil, and to facilitate the development of a pharmacovigilance service. METHODS: A prospective study was conducted at HGP over a period of 8 months, from January 2009 to August 2009. This observational, cross-sectional, descriptive study was based on an analysis of medical records. Several parameters were utilised in the data evaluation, including patient demographics, drug and reaction characteristics, and reaction outcomes. The reaction severity and predisposing factors were also assessed. RESULTS: The overall incidence of ADRs in the patient population was 3.1%, and gender was not found to be a risk factor. The highest ADR rate (75.8%) was found in the adult age group 15 to 50 years, and the lowest ADR rate was found in children aged 3 to 13 years (7.4%). Because of the high frequency of ADRs in orthopaedic (25%), general medicine (22%), and oncology (16%) patients, improved control of the drugs used in these specialties is required. Additionally, the nurse team (52.7%) registered the most ADRs in medical records, most likely due to the job responsibilities of nurses. As expected, the most noticeable ADRs occurred in skin tissues, with such ADRs are more obvious to medical staff, with rashes being the most common reactions. Metamizole, tramadol, and vancomycin were responsible for 21, 11.6, and 8.4% of ADRs, respectively. The majority of ADRs had moderate severity (58.9%), thus requiring intervention. Type A reactions were the most common (82.1%). At least one predisposing factor was present in 79.9% of the reports examined, and the most common predisposing factor was polypharmacy. CONCLUSIONS: The results obtained will contribute to the development of strategies for the pharmacovigilance service at HGP and other hospitals throughout the country, which will improve the quality of ADR reporting and ensure safer drug use in Brazilian hospitals.


Assuntos
Sistemas de Notificação de Reações Adversas a Medicamentos/estatística & dados numéricos , Farmacovigilância , Centros de Atenção Terciária/estatística & dados numéricos , Adolescente , Adulto , Analgésicos Opioides/efeitos adversos , Antibacterianos/efeitos adversos , Anti-Inflamatórios não Esteroides/efeitos adversos , Brasil/epidemiologia , Criança , Pré-Escolar , Dipirona/efeitos adversos , Efeitos Colaterais e Reações Adversas Relacionados a Medicamentos/epidemiologia , Feminino , Hospitalização/estatística & dados numéricos , Humanos , Lactente , Recém-Nascido , Masculino , Pessoa de Meia-Idade , Tramadol/efeitos adversos , Vancomicina/efeitos adversos , Adulto Jovem
15.
Epidemiol. serv. saúde ; 22(2): 243-254, 2013. graf, tab
Artigo em Português | LILACS | ID: lil-682085

RESUMO

Objetivo: descrever os acidentes de trabalho (AT) fatais no Estado do Tocantins, Brasil, e avaliar a qualidade das informações contidas no Sistema de Informações sobre Mortalidade (SIM). Métodos: estudo ecológico descritivo com dados do SIM, no período 2000-2010. Resultados: foram identificados 400 óbitos por AT, com predomínio de trabalhadores do sexo masculino, entre 20 e 49 anos de idade, ocupados nos setores agropecuário, dos transportes e da construção civil; o coeficiente de mortalidade manteve-se estável (p=0,56) no período, equivalente a 6,7 e 9,6/100 mil trabalhadores, em 2000 e 2010, respectivamente; apesar da diminuição da proporção de óbitos por causas mal definidas (de 21,1 por cento em 2000 para 2,7 por cento em 2010), a completitude da informação sobre a relação do óbito com o trabalho permaneceu inferior a 20 por cento no período. Conclusão: o perfil apresentado pode não refletir o real universo dos óbitos por AT, evidenciando a necessidade de aprimorar a qualidade da informação.


Objective: to characterize fatal occupational accidents in the Brazilian State of Tocantins and evaluate Mortality Information System (SIM) information quality. Methods: descriptive ecological study of SIM data between 2000 and 2010. Results: 400 fatal occupational accidents were reported in this study, predominantly males aged 20 to 49 working in the farming, transport and civil construction sectors. The mortality coefficient was stable (p=0.56) in the period at 6.7 and 9.6/100,000 workers in 2000 and 2010 respectively. Despite the reduction in the proportion of deaths with ill-defined causes, the completeness of information on the relationship between death and occupation remained below 20 per cent in the period. Conclusion: the profile found may not reflect the actual universe of fatal occupational accidents, demonstrating the need to improve information quality.


Assuntos
Humanos , Masculino , Feminino , Pré-Escolar , Criança , Adolescente , Adulto , Pessoa de Meia-Idade , Mortalidade Ocupacional , Saúde do Trabalhador/estatística & dados numéricos , Sistemas de Informação , Distribuição Temporal
16.
BMC Biotechnol ; 12: 44, 2012 Aug 02.
Artigo em Inglês | MEDLINE | ID: mdl-22857259

RESUMO

BACKGROUND: ArtinM is a d-mannose-specific lectin from Artocarpus integrifolia seeds that induces neutrophil migration and activation, degranulation of mast cells, acceleration of wound healing, induction of interleukin-12 production by macrophages and dendritic cells, and protective T helper 1 immune response against Leishmania major, Leishmania amazonensis and Paracoccidioides brasiliensis infections. Considering the important biological properties of ArtinM and its therapeutic applicability, this study was designed to produce high-level expression of active recombinant ArtinM (rArtinM) in Escherichia coli system. RESULTS: The ArtinM coding region was inserted in pET29a(+) vector and expressed in E. coli BL21(DE3)-Codon Plus-RP. The conditions for overexpression of soluble ArtinM were optimized testing different parameters: temperatures (20, 25, 30 or 37°C) and shaking speeds (130, 200 or 220 rpm) during induction, concentrations of the induction agent IPTG (0.01-4 mM) and periods of induction (1-19 h). BL21-CodonPlus(DE3)-RP cells induced under the optimized conditions (incubation at 20°C, at a shaking speed of 130 rpm, induction with 0.4 mM IPTG for 19 h) resulted in the accumulation of large amounts of soluble rArtinM. The culture provided 22.4 mg/L of rArtinM, which activity was determined by its one-step purification through affinity chromatography on immobilized d-mannose and glycoarray analysis. Gel filtration showed that rArtinM is monomeric, contrasting with the tetrameric form of the plant native protein (jArtinM). The analysis of intact rArtinM by mass spectrometry revealed a 16,099.5 Da molecular mass, and the peptide mass fingerprint and esi-cid-ms/ms of amino acid sequences of peptides from a tryptic digest covered 41% of the total ArtinM amino acid sequence. In addition, circular dichroism and fluorescence spectroscopy of rArtinM indicated that its global fold comprises ß-sheet structure. CONCLUSIONS: Overall, the optimized process to express rArtinM in E. coli provided high amounts of soluble, correctly folded and active recombinant protein, compatible with large scale production of the lectin.


Assuntos
Escherichia coli/metabolismo , Lectinas de Ligação a Manose/metabolismo , Sequência de Aminoácidos , Animais , Células Cultivadas , Cromatografia de Afinidade , Dicroísmo Circular , Clonagem Molecular , Vetores Genéticos/genética , Vetores Genéticos/metabolismo , Interleucina-12/metabolismo , Isopropiltiogalactosídeo/metabolismo , Lectinas de Ligação a Manose/química , Lectinas de Ligação a Manose/genética , Espectrometria de Massas , Camundongos , Camundongos Endogâmicos C57BL , Dados de Sequência Molecular , Mapeamento de Peptídeos , Estrutura Secundária de Proteína , Proteínas Recombinantes/biossíntese , Proteínas Recombinantes/química , Proteínas Recombinantes/genética , Baço/citologia , Baço/metabolismo , Temperatura
17.
Cien Saude Colet ; 17(6): 1649-56, 2012 Jun.
Artigo em Português | MEDLINE | ID: mdl-22699655

RESUMO

This work aims to evaluate the activity of Lafoensia Pacari and Brossimum gaudichaudii on yeast of the Candida variety isolated from vaginal mucus. The yeasts were obtained from swabs of women with or without symptoms. Susceptibility testing in duplicate was carried out for 34 strains of Candida compared to crude extracts of plant species at concentrations of 50, 100 and 200 mg.mL⁻¹. Extracts that produced inhibition zones with an average of over 10 mm were considered to be active. Antifungal activity of B. gaudichaudii at a concentration of 200-mg.mL⁻¹ was proven, while that of L. pacari was found to be active at 50 mg.mL⁻¹. The activity of plant extracts was revealed compared to Nystatin cream (100.000UI/4g) used for control purposes.


Assuntos
Candida/efeitos dos fármacos , Misturas Complexas/farmacologia , Extratos Vegetais/farmacologia
18.
Ciênc. saúde coletiva ; 17(6): 1649-1656, jun. 2012. tab
Artigo em Português | LILACS | ID: lil-626688

RESUMO

Objetivou-se conhecer a atividade de Lafoensia pacari e a de Brossimum gaudichaudii, sobre leveduras do gênero Candida isoladas da mucosa vaginal. As leveduras foram isoladas a partir de esfregaço de mucosa vaginal de mulheres com ou sem sintomatologia. Realizou-se os testes de susceptibilidade em duplicata para 34 linhagens de Candida frente aos extratos brutos das espécies vegetais, nas concentrações de 50, 100 e 200 mg.mL-1. Consideraram-se como ativos os extratos que produziram halos de inibição com média a partir de 10 mm. Evidenciou-se atividade antifúngica de B. gaudichaudii na concentração de 200 mg.mL-1, enquanto que a de L. pacari mostrou-se ativo a 50 mg.mL-1. A atividade dos extratos vegetais estudados destacou-se em relação à Nistatina creme (100.000UI/4g) utilizada como controle.


This work aims to evaluate the activity of Lafoensia Pacari and Brossimum gaudichaudii on yeast of the Candida variety isolated from vaginal mucus. The yeasts were obtained from swabs of women with or without symptoms. Susceptibility testing in duplicate was carried out for 34 strains of Candida compared to crude extracts of plant species at concentrations of 50, 100 and 200 mg.mL-1. Extracts that produced inhibition zones with an average of over 10 mm were considered to be active. Antifungal activity of B. gaudichaudii at a concentration of 200-mg.mL-1 was proven, while that of L. pacari was found to be active at 50 mg.mL-1. The activity of plant extracts was revealed compared to Nystatin cream (100.000UI/4g) used for control purposes.


Assuntos
Candida/efeitos dos fármacos , Misturas Complexas/farmacologia , Extratos Vegetais/farmacologia
19.
Cell Motil Cytoskeleton ; 65(6): 441-56, 2008 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-18330901

RESUMO

Nuclear actin and nuclear myosins have been implicated in the regulation of gene expression in vertebrate cells. Myosin V is a class of actin-based motor proteins involved in cytoplasmic vesicle transport and anchorage, spindle-pole alignment and mRNA translocation. In this study, myosin-Va, phosphorylated on a conserved serine in the tail domain (phospho-ser(1650) MVa), was localized to subnuclear compartments. A monoclonal antibody, 9E6, raised against a peptide corresponding to phosphoserine(1650) and flanking regions of the murine myosin Va sequence, was immunoreactive to myosin Va heavy chain in cellular and nuclear extracts of HeLa cells, PC12 cells and B16-F10 melanocytes. Immunofluorescence microscopy with this antibody revealed discrete irregular spots within the nucleoplasm that colocalized with SC35, a splicing factor that earmarks nuclear speckles. Phospho-ser(1650) MVa was not detected in other nuclear compartments, such as condensed chromatin, Cajal bodies, gems and perinucleolar caps. Although nucleoli also were not labeled by 9E6 under normal conditions, inhibition of transcription in HeLa cells by actinomycin D caused the redistribution of phospho-ser(1650) MVa to nucleoli, as well as separating a fraction of phospho-ser(1650) MVa from SC35 into near-neighboring particles. These observations indicate a novel role for myosin Va in nuclear compartmentalization and offer a new lead towards the understanding of actomyosin-based gene regulation.


Assuntos
Nucléolo Celular/metabolismo , Núcleo Celular/metabolismo , Proteínas Motores Moleculares/fisiologia , Miosina Tipo V/metabolismo , Actinas/metabolismo , Actinas/ultraestrutura , Animais , Linhagem Celular Tumoral , Nucléolo Celular/ultraestrutura , Núcleo Celular/ultraestrutura , Células HeLa , Humanos , Camundongos , Miosina Tipo V/química , Miosina Tipo V/ultraestrutura , Fosforilação , Ratos , Serina/metabolismo , Transcrição Genética
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