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1.
Endocrinol. diabetes nutr. (Ed. impr.) ; 67(7): 446-453, ago.-sept. 2020. tab
Artigo em Espanhol | IBECS-Express | IBECS | ID: ibc-ET2-6636

RESUMO

ANTECEDENTES: Los receptores CB2R han sido etiquetados como los receptores endocanabinoides periféricos por excelencia, y regulan diferentes procesos inflamatorios. Las variantes del gen CB2R podrían desempeñar un papel en los cambios metabólicos después de la pérdida de peso con diferentes intervenciones. OBJETIVO: Evaluar el efecto de la variante genética (rs3123554) del gen CB2R en los cambios antropométricos y bioquímicos después de la pérdida de peso, secundaria a una dieta hipocalórica alta proteína/baja en hidratos de carbono vs. una dieta hipocalórica estándar durante 9 meses. DISEÑO: Doscientos sesenta y ocho sujetos obesos fueron asignados al azar a una de 2 dietas durante 9 meses, dieta HP (dieta hipocalórica alta en proteínas y baja en hidratos de carbono) y dieta S (dieta hipocalórica estándar en proteínas). Se evaluaron parámetros bioquímicos y antropométricos al basal, a los 3 y a los 9 meses. RESULTADOS: Un total de 94 pacientes (35,1%) tenían el genotipo GG y 174 (64,9%) sujetos tenían los genotipos siguientes; GA (115 pacientes, 42,9%) o AA (59 sujetos de estudio, 18,0%) (segundo grupo). Después de ambas dietas y en ambos genotipos, el índice de masa corporal (IMC), la masa grasa, el peso, la circunferencia de la cintura y la presión arterial sistólica mejoraron sin diferencias entre ambas dietas. Antes y después de ambas dietas hipocalóricas, el peso corporal, el IMC, la masa grasa y la circunferencia de la cintura fueron mayores en los portadores del alelo A. Después de ambas dietas (HP y S), la glucosa, los niveles de insulina, HOMA-IR, los triglicéridos, el colesterol total y el colesterol-LDL disminuyeron en portadores de alelos no A. Estos parámetros se mantuvieron sin cambios en los portadores de alelos A. Los niveles de leptina disminuyeron después de las dietas HP y S en ambos genotipos. CONCLUSIÓN: Los no portadores del alelo A mostraron una mejor respuesta del colesterol total, colesterol LDL, triglicéridos, glucosa, HOMA-IR y niveles de insulina que los portadores A con ambas dietas hipocalóricas y con la misma pérdida de peso


BACKGROUND: CB2R receptors has been referred to as the peripheral cannabinoid receptor isoform, and regulate inflammatory response in various settings. CB2R gene variants could play a role on metabolic changes after weight loss with different interventions. OBJECTIVE: To assess the effect of the genetic variant (rs3123554) of the CB2R gene on anthropometric and biochemical changes after weight loss secondary to a high protein/low carbohydrate diet vs. a standard low-calorie diet during 9 months. DESIGN: 268 obese subjects were randomly allocated to one of two diets for 9 months, Diet HP (high protein-low carbohydrate low-calorie diet) and Diet S (standard protein low-calorie diet). Biochemical and anthropometric parameters were measured at baseline and at 3 and 9 months. RESULTS: Ninety-four patients (35.1%) had genotype GG and 174 (64.9%) subjects had the following genotypes; GA (115 patients, 42.9%) or AA (59 study subjects, 18.0%) (second group). After both diets, body mass index (BMI), fat mass, weight, waist circumference, and systolic blood pressure improved in both genotypes with no difference between diets. Before and after both low-calorie diets, body weight, BMI, fat mass, and waist circumference were higher in A allele carriers than in non-A allele carriers. After both diets (HP and S), levels of glucose, insulin, HOMA-IR, triglycerides, total cholesterol, and LDL cholesterol decreased in non-A allele carriers. These parameters remained unchanged in A allele carriers. Leptin levels decreased after HP and S diets in both genotypes. CONCLUSION: Non-A allele carriers showed a better response of total cholesterol, LDL cholesterol, triglycerides, glucose, HOMA-IR, and insulin levels than allele A carriers with both low-calorie diets and with the same weight loss

2.
Nutr. hosp ; 37(3): 465-473, mayo-jun. 2020. tab, graf
Artigo em Inglês | IBECS-Express | IBECS | ID: ibc-ET2-6103

RESUMO

INTRODUCTION: the composition of snack foods likely influences the overall effect that snacking has on metabolism and obesity. The objective of the current study was to assess the responses to two different snacks, one of them supplemented with wakame and carobs, on cardiovascular risk factors, satiety, and subsequent food intake in obese subjects with metabolic syndrome. MATERIAL AND METHODS: forty patients were randomized in a clinical trial (NCT03420989, clinicaltrial.gov) to group I (enriched snack, n = 16) or group II (control snack, n = 16). At baseline and after 8 weeks biochemical parameters, dietary intakes, and nutritional status were assessed. The subjects also rated their feelings of satiety/hunger with a test meal. RESULTS: no differences were detected in anthropometric parameters between both snacks. Changes in other parameters were detected in patients with enriched snacks, with a significant decrease in LDL-cholesterol by 7.4 % (intervention snack, -8.9 ± 2.3 mg/dL vs control snack, -0.9 ± 3.3 mg/dL; p = 0.03), in total cholesterol by 5.8 % (intervention snack, -10.4 ± 2.9 mg/dL vs control snack, -1.4 ± 3.2 mg/dL; p = 0.02), and in resistin level by 15.9 % (intervention snack, -1.0 ± 0.2 mg/dL vs control snack, -0.1 ± 0.3 mg/dL: p = 0.03). After the test meal, satiety scores (after 20 min and 40 min) were higher than fasting levels in both groups. The same results were obtained with the 100-mm, 5-point visual satiety scale. CONCLUSION: our study indicates that a wakame- and carob-enriched snack induces a significant decrease in total cholesterol, LDL-cholesterol, and resistin levels when compared to a control snack, without effects on food consumption, other cardiovascular parameters, or anthropometric parameters


INTRODUCCIÓN: la composición de los "snacks" probablemente influya en el efecto que produce su consumo sobre los marcadores metabólicos y la obesidad. El objetivo del presente estudio fue evaluar las respuestas a dos snacks, uno de ellos suplementado con wakame y algarroba, sobre factores de riesgo cardiovascular, saciedad y posterior ingesta de alimentos, en sujetos obesos con síndrome metabólico. MATERIAL Y MÉTODOS: se aleatorizaron 40 pacientes en el ensayo clínico NCT03420989 (clinicaltrial.gov) para participar en el grupo I (snack enriquecido, n = 16) o el grupo II (snack de control, n = 16). Antes y después de 8 semanas se determinaron los parámetros bioquímicos, las ingestas dietéticas y el estado nutricional. A los sujetos también se les evaluó la saciedad y el apetito con una comida de prueba. RESULTADOS: no se detectaron diferencias en los parámetros antropométricos con ambos snacks. Se detectaron cambios en los parámetros bioquímicos de los pacientes que recibieron snacks enriquecidos, con una disminución significativa del colesterol-LDL del 7,4 % (snack de intervención, -8,9 ± 2,3 mg/dl vs. snack de control, -0,9 ± 3,3 mg/dl; p = 0,03), del colesterol total del 5,8 % (snack de intervención, -10,4 ± 2,9 mg/dl vs. snack de control, -1,4 ± 3,2 mg/dl; p = 0,02) y de los niveles de resistina del 15,9 % (snack de intervención, -1,0 ± 0,2 mg/dl vs. snack de control, -0,1 ± 0,3 mg/dl; p = 0,03). Después de la comida de prueba, las puntuaciones de saciedad (a los 20 min y 40 min) fueron más altas que el nivel de ayuno en ambos grupos. Los resultados fueron similares con la escala de saciedad visual de 5 puntos y 100 mm. CONCLUSIÓN: nuestro estudio muestra que un snack enriquecido con wakame y algarroba produce una disminución significativa de los niveles de colesterol total, colesterol-LDL y resistina frente a un snack de control, sin efectos sobre el consumo de alimentos, otros parámetros cardiovasculares y los parámetros antropométricos

3.
Nutr Hosp ; 34(3): 465-473, 2020 Jul 13.
Artigo em Inglês | MEDLINE | ID: mdl-32379474

RESUMO

Introduction: Introduction: the composition of snack foods likely influences the overall effect that snacking has on metabolism and obesity. The objective of the current study was to assess the responses to two different snacks, one of them supplemented with wakame and carobs, on cardiovascular risk factors, satiety, and subsequent food intake in obese subjects with metabolic syndrome. Material and Methods: forty patients were randomized in a clinical trial (NCT03420989, clinicaltrial.gov) to group I (enriched snack, n = 16) or group II (control snack, n = 16). At baseline and after 8 weeks biochemical parameters, dietary intakes, and nutritional status were assessed. The subjects also rated their feelings of satiety/hunger with a test meal. Results: no differences were detected in anthropometric parameters between both snacks. Changes in other parameters were detected in patients with enriched snacks, with a significant decrease in LDL-cholesterol by 7.4 % (intervention snack, -8.9 ± 2.3 mg/dL vs control snack, -0.9 ± 3.3 mg/dL; p = 0.03), in total cholesterol by 5.8 % (intervention snack, -10.4 ± 2.9 mg/dL vs control snack, -1.4 ± 3.2 mg/dL; p = 0.02), and in resistin level by 15.9 % (intervention snack, -1.0 ± 0.2 mg/dL vs control snack, -0.1 ± 0.3 mg/dL: p = 0.03). After the test meal, satiety scores (after 20 min and 40 min) were higher than fasting levels in both groups. The same results were obtained with the 100-mm, 5-point visual satiety scale. Conclusion: our study indicates that a wakame- and carob-enriched snack induces a significant decrease in total cholesterol, LDL-cholesterol, and resistin levels when compared to a control snack, without effects on food consumption, other cardiovascular parameters, or anthropometric parameters.

4.
Endocrine ; 68(3): 557-563, 2020 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-32239453

RESUMO

BACKGROUND AND AIMS: The prevalence of the non-alcoholic fatty liver disease (NAFLD) in developed countries is up to 30% of the general population, and 50% of patients present type 2 diabetes mellitus (DM2). Fibrosis is the most important prognostic factor in NAFLD. The aim of this study was to search evidence for an early diagnosis of liver fibrosis in subjects with DM2 and to evaluate potential risk and protective factors. METHODS: This study was conducted among 160 diabetic patients with NAFLD proven biopsy. Anthropometric assessments, laboratory test, liver histological features and follow-up of a Mediterranean diet were evaluated. RESULTS: Diabetic patients with liver fibrosis showed a greater number of positive metabolic criteria than diabetic patients without liver fibrosis. Patients with hepatic fibrosis have a lower score on the PREDIMED test (9.0 (2.4) vs. 6.2 (2.3); p < 0.05). Diabetic patients with liver fibrosis showed higher glucose levels (delta: 10.1 (4.5) mg/dl), fasting insulin levels (delta: 3.1 (1.5) UI/L), HOMA-IR (delta: 2.1 (0.3) units) and HbA1c (delta: 0.6 (0.2)%). Non-invasive tests showed a higher score (non-alcoholic fatty liver disease fibrosis score and fibrosis-4) in liver fibrosis subjects than no liver fibrosis subjects. A logistic regression analysis adjusted by age, gender, HbA1c and body mass index showed independent significant direct association between liver fibrosis and homeostatic model assessment of insulin resistance as indicator of insulin resistance (odds ratio (OR) = 1.53: 95% confidence interval (CI): 1.1-2.2; p = 0.026) and inverse association with PREDIMED score as an indicator of adherence to Mediterranean diet (OR = 0.6; 95% CI: 0.4-0.8; p = 0.01). CONCLUSION: In patients with DM2, insulin resistance is an independent risk factor associated with liver fibrosis, and the adherence of a Mediterranean diet is a protective factor associated with absence of liver fibrosis.

5.
J Diabetes Complications ; 34(4): 107534, 2020 04.
Artigo em Inglês | MEDLINE | ID: mdl-32057567

RESUMO

BACKGROUND & AIMS: The risk allele (G) of rs10830963 in the melatonin receptor 1 B (MTNR1B) gene presents an association with biochemical parameters and obesity. We study the effect of this SNP on insulin resistance and weight loss secondary to two hypocaloric diets. METHODS: 270 obese subjects were randomly allocated during 9 months (Diet HP: a high protein/low carbohydrate vs. Diet S: a standard severe hypocaloric diets). Anthropometric parameters, fasting blood glucose, C-reactive protein (CRP), insulin concentration, insulin resistance (HOMA-IR), lipid profile and adipocytokines levels were measured. Genotype of MTNR1B gene polymorphism (rs10830963) was evaluated. RESULTS: All adiposity parameters, systolic blood pressure and leptin levels decreased in all subjects after both diets. This improvement of adiposity parameters was higher in non-G allele carriers than G allele carriers. After weight loss with Diet HP, (CC vs. CG + GG at 9 months); total cholesterol (delta: -9.9 ±â€¯2.4 mg/dl vs. -4.8 ±â€¯2.2 mg/dl:p < 0.05), LDL-cholesterol (delta: -8.3 ±â€¯1.9 mg/dl vs. -5.1 ±â€¯2.2 mg/dl: p < 0.05), insulin (delta: -4.7 ±â€¯0.8 UI/L vs. -0.9 ±â€¯1.0 UI/L: p < 0.05), triglycerides (delta: -17.7 ±â€¯3.9 mg/dl vs. -6.1 ±â€¯2.8 mg/dl: p < 0.05) and HOMA IR (delta: -0.8 ±â€¯0.2 units vs. -0.2 ±â€¯0.1 units: p < 0.05) improved only in no G allele carriers. After weight loss with Diet S in non G allele carriers, insulin levels (delta (CC vs. CG + GG): -3.4 ±â€¯0.6 UI/L vs. -1.2 ±â€¯0.4 UI/L: p < 0.05), triglycerides (delta: -29.2 ±â€¯3.4 mg/dl vs. -8.2 ±â€¯3.8 mg/dl: p < 0.05), HOMA-IR (delta (CC vs. CG + GG): -1.1 ±â€¯0.2 units vs. -0.1 ±â€¯0.1 units: p < 0.05), total cholesterol (delta: -15.9 ±â€¯7.4 mg/dl vs. -5.8 ±â€¯2.9 mg/dl:ns) and LDL-cholesterol (delta: -13.7 ±â€¯5.9 mg/dl vs. -6.0 ±â€¯2.9 mg/dl: ns) decreased, too. CONCLUSIONS: our study detected a relationship of rs10830963 variant of MTNR1B gene with adiposity changes, cholesterol changes and insulin resistance modification induced by two different hypocaloric during 9 months.

6.
Endocrinol. diabetes nutr. (Ed. impr.) ; 67(1): 43-52, ene. 2020. tab
Artigo em Espanhol | IBECS | ID: ibc-186146

RESUMO

ANTECEDENTES Y OBJETIVOS: El alelo de riesgo (G) de la variante rs10830963 en el gen del receptor de melatonina 1 B (MTNR1B) se relaciona con la obesidad. En este trabajo evaluamos el efecto de este SNP sobre los factores de riesgo cardiovascular y la pérdida de peso secundaria a 2 dietas hipocalóricas. MÉTODOS: Trescientos sesenta y un sujetos obesos fueron asignados aleatoriamente durante 3 meses (dieta M: dieta hipocalórica alta en grasas monoinsaturadas vs. dieta P: dieta hipocalórica alta en grasas poliinsaturadas). Se midieron los parámetros antropométricos, glucemia en ayunas, proteína C reactiva, concentración de insulina, resistencia a la insulina (HOMA-IR), perfil de lípidos y los niveles de adipocitoquinas. Se evaluó el genotipo del polimorfismo del gen MTNR1B (rs10830963). RESULTADOS: Todos los parámetros antropométricos, la presión arterial sistólica y los niveles de leptina disminuyeron en todos los sujetos después de ambas dietas. Esta mejora de los parámetros antropométricos fue mayor en los no portadores del alelo G que en los portadores del alelo G. Tras la intervención con dieta M (CC vs. CG + GG), el colesterol total (delta: -10,4 ± 2,1 mg/dl vs. -6,4 ± 1,2 mg/dl: p < 0,05), colesterol LDL (delta: -7,1 ± 0,9 mg/dl vs. -2,8 ± 0,8 mg/dl: p < 0,05), insulina (delta: -3,0 ± 0.8 UI/l vs. -2,0 ± 1,0 UI/l: p < 0,05) y HOMA IR (delta: -3,4 ± 1,0 unidades vs. -2,9 ± 0,9 unidades: p < 0,05) mejoraron en los no portadores del alelo G. Tras la dieta P, en el grupo de sujetos sin alelo G, los niveles de insulina (delta: -2,9 ± 1,0 UI/l vs. -0,6 ± 0,2 UI/l: p < 0,05) y HOMA-IR (delta [CC vs. CG + GG]: -0,8 ± 0,2 unidades vs. -0,4 ± 0,3 unidades: p < 0,05) también disminuyeron. CONCLUSIONES: Nuestro estudio detectó una relación de la variante rs10830963 de MTNR1B con la pérdida de peso corporal y la modificación de la resistencia a la insulina inducida por 2 dietas hipocalóricas diferentes. Solo la dieta hipocalórica enriquecida en grasa monoinsaturada y los no portadores del alelo G mostraron un efecto significativo sobre las lipoproteínas


BACKGROUND & AIMS: The risk allele (G) of rs10830963 in the melatonin receptor 1 B (MTNR1B) gene presents an association with obesity. We study the effect of this SNP on cardiovascular risk factors and weight loss secondary to 2hypocaloric diets. Methods: 361 obese subjects were randomly allocated during 3 months (Diet M - high monounsaturated fat hypocaloric diet vs. Diet P - high polyunsaturated fat hypocaloric diet). Anthropometric parameters, fasting blood glucose, C-reactive protein (CRP), insulin concentration, insulin resistance (HOMA-IR), lipid profile and adipocytokines levels were measured. Genotype of MTNR1B gene polymorphism (rs10830963) was evaluated. Results: All anthropometric parameters, systolic blood pressure and leptin levels decreased in all subjects after both diets. This improvement of anthropometric parameters was higher in non G allele carriers than G allele carriers. After dietary intervention with Diet M, (CC vs. CG + GG); total cholesterol (delta: -10.4 ± 2.1 mg/dl vs. -6.4 ± 1.2 mg/dl: P <.05), LDL-cholesterol (delta:-7.1 ± 0.9 mg/dl vs. -2.8 ± 0.8 mg/dl: P <.05), insulin (delta:-3.0 ± 0.8 UI/L vs. -2.0 ± 1.0 UI/L: P<.05) and HOMA-IR (delta:-3.4 ± 1.0 units vs. -2.9 ± 0.9 units: P<.05) improved in no G allele carriers. After Diet P, in the group of subjects without G allele CC, insulin levels (delta: -2.9 ± 1.0 UI/L vs. -0.6 ± 0.2 UI/L: P <.05) and HOMA-IR (delta (CC vs. CG + GG): -0.8 ± 0.2 units vs. -0.4 ± 0.3 units: P <.05) decreased, too. Conclusions: Our study detected a relationship of rs10830963 MTNR1B SNP with body weight loss and insulin resistance modification induced by 2 different hypocaloric. Only monounsaturated enriched hypocaloric diet and in no-G allele carriers showed a significant effect on lipoproteins


Assuntos
Humanos , Adolescente , Adulto Jovem , Adulto , Pessoa de Meia-Idade , Idoso , Polimorfismo Genético , Gorduras na Dieta/uso terapêutico , Resistência à Insulina , Perda de Peso/efeitos dos fármacos , Obesidade/dietoterapia , Fatores de Risco , Gorduras na Dieta/metabolismo , Doenças Cardiovasculares , Antropometria , Glicemia , Composição Corporal , Relação Cintura-Quadril , Radioimunoensaio/métodos
7.
Lifestyle Genom ; 13(1): 20-27, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-31747677

RESUMO

BACKGROUND: The role of ADIPOQ gene variants in weight loss after different dietary fat amounts remains unclear. OBJECTIVE: Our aim was to analyze the effects of ADIPOQ gene polymorphism rs266729 on metabolic changes after two different amounts of dietary fat in two hypocaloric diets. DESIGN: A population of 283 obese patients was recruited in a randomized clinical trial with two diets: Diet HF (high-fat diet: 38% carbohydrates, 24% proteins, and 38% fats) versus Diet LF (low-fat diet: 53% carbohydrates, 20% proteins, and 27% fats). Before and after 3 months, an anthropometric evaluation, an assessment of nutritional intake, and a biochemical analysis were carried out. The variant of the ADIPOQgene was assessed by real-time PCR. RESULTS: Weight loss was similar with both diets in both genotypes (CC vs. CG+GG). After dietary intervention with Diet HF, only subjects with CC genotype showed a significant improvement in insulin levels (-3.3 ± 0.6 vs. -1.8 ± 0.9 mU/L; p = 0.03) and the homeostasis model assessment for insulin resistance (HOMA-IR) (-1.3 ± 0.1 vs. -0.8 ± 0.2 units; p = 0.02). After Diet LF, subjects with CC genotype showed a significant improvement in total cholesterol levels (CC vs. CG+GG) (-15.3 ± 1.4 vs. -6.4 ± 1.3 mg/dL; p = 0.01), LDL cholesterol (-14.6 ± 1.8 vs. -6.4 ± 1.3 mg/dL; p = 0.01), insulin levels (-4.6 ± 1.0 vs. -1.6 ± 0.5 mU/L; p = 0.01), and HOMA-IR (-1.6 ± 0.1 vs. -1.0 ± 0.2 units; p = 0.02). Only subjects with CC genotype showed a significant increase of adiponectin levels after both diets (CC vs. CG+GG): Diet HF (10.6 ± 2.0 vs. 1.8 ± 1.0 ng/dL; p = 0.01) and Diet LF (16.1 ± 2.8 vs. 1.3 ± 1.0 ng/dL: p = 0.03). CONCLUSION: CC genotype of ADIPOQgene variantrs266729 was associated with a better metabolic response after both diets. Additionally, Diet LF produced a significant improvement in lipid profile in noncarriers of allele G.

8.
Endocrinol Diabetes Nutr ; 67(1): 43-52, 2020 Jan.
Artigo em Inglês, Espanhol | MEDLINE | ID: mdl-30981681

RESUMO

BACKGROUND & AIMS: The risk allele (G) of rs10830963 in the melatonin receptor 1 B (MTNR1B) gene presents an association with obesity. We study the effect of this SNP on cardiovascular risk factors and weight loss secondary to 2hypocaloric diets. METHODS: 361 obese subjects were randomly allocated during 3 months (Diet M - high monounsaturated fat hypocaloric diet vs. Diet P - high polyunsaturated fat hypocaloric diet). Anthropometric parameters, fasting blood glucose, C-reactive protein (CRP), insulin concentration, insulin resistance (HOMA-IR), lipid profile and adipocytokines levels were measured. Genotype of MTNR1B gene polymorphism (rs10830963) was evaluated. RESULTS: All anthropometric parameters, systolic blood pressure and leptin levels decreased in all subjects after both diets. This improvement of anthropometric parameters was higher in non G allele carriers than G allele carriers. After dietary intervention with Diet M, (CC vs. CG + GG); total cholesterol (delta: -10.4 ± 2.1mg/dl vs. -6.4 ± 1.2mg/dl: P <.05), LDL-cholesterol (delta:-7.1 ± 0.9mg/dl vs. -2.8 ± 0.8mg/dl: P <.05), insulin (delta:-3.0 ± 0.8 UI/L vs. -2.0 ± 1.0 UI/L: P<.05) and HOMA-IR (delta:-3.4 ± 1.0 units vs. -2.9 ± 0.9 units: P<.05) improved in no G allele carriers. After Diet P, in the group of subjects without G allele CC, insulin levels (delta: -2.9 ± 1.0 UI/L vs. -0.6 ± 0.2 UI/L: P <.05) and HOMA-IR (delta (CC vs. CG + GG): -0.8 ± 0.2 units vs. -0.4 ± 0.3 units: P <.05) decreased, too. CONCLUSIONS: Our study detected a relationship of rs10830963 MTNR1B SNP with body weight loss and insulin resistance modification induced by 2different hypocaloric. Only monounsaturated enriched hypocaloric diet and in no-G allele carriers showed a significant effect on lipoproteins.

9.
Clin Nutr ; 39(4): 988-993, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-31080037

RESUMO

BACKGROUND & AIMS: A common G-to-A transition (rs670) in the APOA1 gene has been related with metabolism. We evaluate the association of this SNP with changes in lipid profile and insulin resistance in response to two diets. METHODS: 268 obese patients were randomly allocated to a high protein/low carbohydrate -Diet HP- vs. a standard hypocaloric diet -Diet S- for 9 months. Anthropometric and biochemical status were evaluated at 3 and 9 months. RESULTS: 179 subjects (66.8%) had the genotype GG, 79 patients GA (29.4%) and 10 subjects AA (3,8%). With both diets: the decrease of BMI, weight, waist circumference, fat mass was higher in A allele carriers than non-carriers. Also on both diets A allele carriers showed greater improvements in total cholesterol (-19.0 ± 2.5 mg/dl (non-A allele carriers -12.1 ± 2.0 mg/dl:p = 0.02 after Diet HP) and -13.1 ± 2.1 mg/dl (non-A allele carriers -8.9 ± 1.1 mg/dl:p = 0.02 after Diet S)), LDL-cholesterol (-18.0 ± 2.1 mg/dl (non-A allele carriers -8.3 ± 2.2 mg/dl:p = 0.01 after Diet HP) and -12.0 ± 1.5 mg/dl (non-A allele carriers -6.3 ± 2.3 mg/dl:p = 0.01 after Diet S)), insulin (-2.5 ± 0.2 mUI/L (in non A allele -1.8 ± 0.2 mUI/L:p = 0.01 after Diet HP) and -2.1 ± 0.1 mUI/L (non A allele carriers -1.2 ± 0.3 mUI/L:p = 0.01 after Diet S)), HOMA-IR (-1.3 ± 0.3 units (non A allele group -0.8 ± 0.2:p = 0.03 after Diet HP) and -1.1 ± 0.1 units (non A allele carriers -0.3 ± 0.2 mg/dl:p = 0.01 after Diet S)) than non-A allele carriers. CONCLUSIONS: A allele carriers of rs670 ApoA1 polymorphism showed a higher decrease of insulin resistance, LDL cholesterol and adiposity induced by two different hypocaloric diet than non A allele carriers.

10.
Artigo em Inglês, Espanhol | MEDLINE | ID: mdl-31839571

RESUMO

BACKGROUND: CB2R receptors has been referred to as the peripheral cannabinoid receptor isoform, and regulate inflammatory response in various settings. CB2R gene variants could play a role on metabolic changes after weight loss with different interventions. OBJECTIVE: To assess the effect of the genetic variant (rs3123554) of the CB2R gene on anthropometric and biochemical changes after weight loss secondary to a high protein/low carbohydrate diet vs. a standard low-calorie diet during 9 months. DESIGN: 268 obese subjects were randomly allocated to one of two diets for 9 months, Diet HP (high protein-low carbohydrate low-calorie diet) and Diet S (standard protein low-calorie diet). Biochemical and anthropometric parameters were measured at baseline and at 3 and 9 months. RESULTS: Ninety-four patients (35.1%) had genotype GG and 174 (64.9%) subjects had the following genotypes; GA (115 patients, 42.9%) or AA (59 study subjects, 18.0%) (second group). After both diets, body mass index (BMI), fat mass, weight, waist circumference, and systolic blood pressure improved in both genotypes with no difference between diets. Before and after both low-calorie diets, body weight, BMI, fat mass, and waist circumference were higher in A allele carriers than in non-A allele carriers. After both diets (HP and S), levels of glucose, insulin, HOMA-IR, triglycerides, total cholesterol, and LDL cholesterol decreased in non-A allele carriers. These parameters remained unchanged in A allele carriers. Leptin levels decreased after HP and S diets in both genotypes. CONCLUSION: Non-A allele carriers showed a better response of total cholesterol, LDL cholesterol, triglycerides, glucose, HOMA-IR, and insulin levels than allele A carriers with both low-calorie diets and with the same weight loss.

11.
Nutr. hosp ; 36(6): 1288-1295, nov.-dic. 2019. tab
Artigo em Espanhol | IBECS | ID: ibc-191147

RESUMO

Antecedentes y objetivos: las variantes genéticas del gen APOA1 se han relacionado con el perfil lipídico en sujetos obesos. Nuestro objetivo fue analizar los efectos del polimorfismo del gen rs670 APOA1 sobre el estado metabólico tras la ingesta de dos dietas hipocalóricas enriquecidas con grasas poliinsaturadas o con grasas monoinsaturadas. Métodos: trescientos sesenta sujetos obesos se asignaron al azar a dos grupos de intervención. Un grupo recibió una dieta enriquecida en grasas poliinsaturadas (dieta P) y el otro grupo una dieta enriquecida en grasas monoinsaturadas (dieta M) durante 12 semanas. Se evaluaron los efectos sobre los biomarcadores relacionados con el metabolismo lipídico y de hidratos de carbono antes y después de la intervención. Resultados: el índice de masa corporal, el peso, la masa grasa, la circunferencia de la cintura, la presión arterial sistólica, las concentraciones plasmáticas de leptina y la circunferencia de la cintura disminuyeron en todos los pacientes tras ambas dietas. En los portadores del alelo A, después de 12 semanas con la dieta P, los niveles de insulina (delta: -7,3 ± 2,2 UI/I; p = 0,01) y HOMA-IR (delta: -2,8 ± 0,5 unidades; p = 0,02) mejoraron de manera significativa. Tras el tratamiento con la dieta M, los niveles plasmáticos de insulina (delta: -5,9 ± 1,2 UI/I; p = 0,01) y HOMAIR (delta: -2,1 ± 0,8 unidades; p = 0,02) también mejoraron en los portadores del alelo A. Después de la intervención dietética con la dieta P, el colesterol-LDL (delta: -12,1 ± 4,3 UI/I; p = 0,01) y el colesterol-HDL (delta: 2,6 ± 0,7 unidades; p = 0,01) disminuyeron significativamente en los portadores del alelo A. Conclusiones: nuestro estudio mostró la asociación del polimorfismo rs670 ApoA1 con los cambios de resistencia a la insulina inducidos por ambas dietas y aportó evidencia adicional sobre la mejoría del colesterol-HDL y el colesterol-LDL después de una dieta rica en grasas poliinsaturadas en los portadores del alelo A


Background and objectives: genetic variants of the APOA1 gene have been related to lipid profile in obese subjects. Our aim was to analyze the effects of the rs670 APOA1 gene polymorphism on metabolic changes secondary to an enriched-polyunsaturated fat vs. an enriched-monounsaturated fat hypocaloric diet. Methods: 360 Caucasian obese subjects were randomly allocated to two groups. One group received an enriched-polyunsaturated fat (diet P) and the other an enriched-monounsaturated fat hypocaloric diet (diet M) during 12 weeks. The effects on serum biomarkers related to lipid and carbohydrate metabolism were evaluated before and after the dietary intervention. Results: after both diets, body mass index, weight, fat mass, waist circumference, systolic blood pressure, plasma leptin concentration, and waist circumference decreased in all patients. After 12 weeks of intervention with diet P, plasma insulin levels and HOMA-IR decreased in A-allele carriers: delta: -7.3 ± 2.2 IU/L (p = 0.01), and delta: -2.8 ± 0.5 units (p = 0.02), respectively. The same changes in delta were observed after diet M in A-allele carriers: insulin delta: -5.9 ± 1.2 IU/L (p = 0.01), and HOMA-IR delta: -2.1 ± 0.8 units (p = 0.02). In A-allele carriers, LDL-cholesterol decreased and HDL-cholesterol increased after the dietary intervention with diet P: delta: -12.1 ± 4.3 mg/dL (p = 0.01), and delta: 2.6 ± 0.7 mg/dL (p = 0.01), respectively. No differences in lipid profile were observed after diet M. These improvements were not observed in non-A-allele carriers after both interventions. Conclusions: our study showed the association of the rs670 ApoA1 polymorphism with insulin resistance changes as induced by both diets. An enriched-polyunsaturated fat diet produced an additional improvement of HDL-cholesterol and LDL-cholesterol in A-allele carriers


Assuntos
Humanos , Adulto , Pessoa de Meia-Idade , Idoso , Apolipoproteína A-I/genética , HDL-Colesterol/sangue , Dieta Redutora , Ácidos Graxos Monoinsaturados/administração & dosagem , Ácidos Graxos Insaturados/administração & dosagem , Obesidade/dietoterapia , Variação Genética , Obesidade/genética , Obesidade/metabolismo
12.
Nutr Hosp ; 36(6): 1288-1295, 2019 Dec 26.
Artigo em Espanhol | MEDLINE | ID: mdl-31682461

RESUMO

Introduction: Background and objectives: genetic variants of the APOA1 gene have been related to lipid profile in obese subjects. Our aim was to analyze the effects of the rs670 APOA1 gene polymorphism on metabolic changes secondary to an enriched-polyunsaturated fat vs. an enriched-monounsaturated fat hypocaloric diet. Methods: 360 Caucasian obese subjects were randomly allocated to two groups. One group received an enriched-polyunsaturated fat (diet P) and the other an enriched-monounsaturated fat hypocaloric diet (diet M) during 12 weeks. The effects on serum biomarkers related to lipid and carbohydrate metabolism were evaluated before and after the dietary intervention. Results: after both diets, body mass index, weight, fat mass, waist circumference, systolic blood pressure, plasma leptin concentration, and waist circumference decreased in all patients. After 12 weeks of intervention with diet P, plasma insulin levels and HOMA-IR decreased in A-allele carriers: delta: -7.3 ± 2.2 IU/L (p = 0.01), and delta: -2.8 ± 0.5 units (p = 0.02), respectively. The same changes in delta were observed after diet M in A-allele carriers: insulin delta: -5.9 ± 1.2 IU/L (p = 0.01), and HOMA-IR delta: -2.1 ± 0.8 units (p = 0.02). In A-allele carriers, LDL-cholesterol decreased and HDL-cholesterol increased after the dietary intervention with diet P: delta: -12.1 ± 4.3 mg/dL (p = 0.01), and delta: 2.6 ± 0.7 mg/dL (p = 0.01), respectively. No differences in lipid profile were observed after diet M. These improvements were not observed in non-A-allele carriers after both interventions. Conclusions: our study showed the association of the rs670 ApoA1 polymorphism with insulin resistance changes as induced by both diets. An enriched-polyunsaturated fat diet produced an additional improvement of HDL-cholesterol and LDL-cholesterol in A-allele carriers.

13.
Diabetes Res Clin Pract ; 156: 107825, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31449874

RESUMO

BACKGROUND AND AIMS: Few studies have assessed the effect of the NPY gene rs16147 variant on metabolic response following a dietary intervention. We evaluated the effect of rs16147 on body weight and biochemical changes after a high-protein/low-carbohydrate hypocaloric diet compared with a standard severe hypocaloric diet over 9 months. MATERIALS AND METHODS: A population of 270 obese individuals was enrolled. At baseline, participants were randomly allocated to one of two hypocaloric diets, high protein (Diet HP) or standard (Diet S), for a period of 9 months. RESULTS: After both diets, all genotypes showed decreased body mass index, weight, fat mass, waist circumference, and leptin levels. Participants with the minor allele (A) assigned to the HP diet showed decreases in total cholesterol (-6.5 ±â€¯4.8 vs 10.1 ±â€¯4.1 mg/dL; p < 0.05), LDL cholesterol (-5.9 ±â€¯3.8 vs 9.6 ±â€¯2.4 mg/dL; p < 0.05), triglycerides (-1.0 ±â€¯4.8 vs 16.2 ±â€¯4.1 mg/dL; p < 0.05), insulin (-0.5 ±â€¯2.8 vs 1.7 ±â€¯2.1 UI/L; p < 0.05), HOMA-IR (-0.2 ±â€¯2.1 vs 0.5 ±â€¯2.0 units; p < 0.05), and CRP (-0.3 ±â€¯0.4 vs 1.3 ±â€¯0.2 mg/dL; p < 0.05). Participants with the minor allele assigned to diet S also showed decreases in total cholesterol (-6.1 ±â€¯4.1 vs 14.4 ±â€¯3.1 mg/dL; p < 0.05), LDL-cholesterol (-3.1 ±â€¯2.8 vs 15.0 ±â€¯3.1 mg/dL; p < 0.05), triglycerides (-6.9 ±â€¯4.1 vs 13.2 ±â€¯4.0 mg/dL; p < 0.05), insulin (-0.3 ±â€¯2.1 vs. -1.2 ±â€¯0.2 UI/L: p < 0.05), HOMA-IR (-0.3 ±â€¯2.1 vs. -1.6 ±â€¯1.1 units: p < 0.05), and CRP (-0.4 ±â€¯0.1 vs 1.1 ±â€¯0.2 mg/dL; p < 0.05). CONCLUSION: In obese Caucasians, the presence of the A allele of the rs16147 genetic variant produces a better metabolic response that is secondary to weight loss with two different hypocaloric diets.


Assuntos
Dieta Rica em Proteínas e Pobre em Carboidratos/métodos , Resistência à Insulina/genética , Neuropeptídeo Y/metabolismo , Obesidade/dietoterapia , Feminino , Humanos , Masculino , Pessoa de Meia-Idade
14.
Nutrition ; 65: 44-49, 2019 09.
Artigo em Inglês | MEDLINE | ID: mdl-31029921

RESUMO

OBJECTIVE: Some adiponectin gene (ADIPOQ) single-nucleotide polymorphisms (SNPs) have been related to basal and adiponectin levels and metabolic parameters. The aim of this study was to evaluate the effect of the genetic variant rs1501299 ADIPOQ gene on biochemical changes after weight loss secondary to a high-protein and low-carbohydrate diet versus a standard severe hypocaloric diet over 9 mo as the primary endpoint. METHODS: A white population of 270 obese patients was enrolled in a randomized clinical trial with two hypocaloric diets (high-protein and low carbohydrate diet [HP] versus standard diet [S]) over 9 mo of intervention. The statistical analysis was performed for the combined GT and TT as a group (T-allele carriers) and GG as second group (non-T-allele carriers). Before and after 12 wk on each hypocaloric diet, an anthropometric evaluation, an assessment of nutritional intake, and a biochemical analysis were realized. RESULTS: With both dietary interventions, body weight, body mass index (BMI), fat mass, waist circumference, systolic blood pressure, and leptin levels decreased. In non-T-allele carriers after both diets, the decrease in total cholesterol levels -12.3 ± 2.2 mg/dL (T-allele carriers -6.9 ± 2.1 mg/dL; P = 0.01 diet HP) and 12.2 ± 3.1 mg/dL (T-allele carriers -4.7 ± 1.2 mg/dL; P = 0.02 after diet S), low-density lipoprotein cholesterol -13.2 ± 2.7 mg/dL (T-allele carriers -6.1 ± 2.1 mg/dL; P = 0.02 after diet HP) and -9.3 ± 1.8 mg/dL (T-allele carriers -4.8 ± 2.9 mg/dL; P = 0.01 after diet S), triacylglycerol levels -12.7 ± 6.1 mg/dL (T-allele carriers -6 ± 2.9 mg/dL; P = 0.01 after diet HP) and -16.3 ± 7.2 mg/dL (T-allele carriers -5.3 ± 1.4 mg/dL; P = 0.03 after diet S), insulin levels -5 ± 1.1 mUI/L (in T-allele -1.7 ± 0.9 mUI/L; P = 0.02 after diet HP) and -3.2 1.1 mUI/L (T-allele carriers -0.7 ± 0.7 mUI/L; P = 0.02 after diet S), and homeostatic model assessment of insulin resistance levels -0.4 ± 0.2 units (T-allele group -0.1 ± 0.1; P = 0.04 after diet HP) and -0.7 ± 0.1 units (T-allele carriers -0.1 ± 0.5 mg/dL; P = 0.01 after diet S) was higher than T-allele carriers. Only no T-allele carriers showed an increase in adiponectin levels after both diets. CONCLUSION: After two different hypocaloric diets during 9 mo of intervention, the GG genotype of an ADIPOQ gene variant (rs1501299) is related to better improvement in adiponectin levels, insulin resistance, and lipid profile than T-allele carriers.

15.
Endocrinol. diabetes nutr. (Ed. impr.) ; 66(4): 217-222, abr. 2019. tab
Artigo em Inglês | IBECS | ID: ibc-183171

RESUMO

Background and rationale: This study was intended to assess the influence of the rs16147 variant of the NPY gene on liver histology in patients with non-alcoholic fatty liver disease (NAFLD). Material and methods: Eighty-nine patients with NAFLD were recruited into the study. Serum chemistry tests were done including lipid profile, transaminases, adipokines, and insulin resistance. Genotype of polymorphism (rs161477) of the NPY gene was studied. Results: Twenty-three patients (25.0%) had the GG genotype (wild type) and sixty-six patients (75%) the GA (n=39) or AA (n=27) (mutant) types. Patients with A allele had a lower percentage of lobular inflammation and steatohepatitis (lobular inflammation plus ballooning) than those with wild genotype. Patients with A allele showed lower SAF (Steatosis, Activity, Fibrosis) scores than non-A allele carriers (5.4±2.7 points vs. 4.1±1.1 points; p=0.01). In the analysis without fibrosis (NAS score), the same differences were detected (4.5±1.8 points vs. 3.4±1.8 points; p=0.01). In the logistic regression analysis A allele carriers showed lower odds for inflammation (OR 0.11, 95% CI 0.02-0.84, p=0.03) and steatohepatitis (OR 0.39, 95% CI 0.14-0.86, p=0.04) after adjusting for age, sex, and body mass index. Conclusions: A variant of polymorphism rs16147 of the NPY gene is independently associated to a lower percentage of steatohepatitis and lobular inflammation in obese subjects with biopsy-proven NAFLD


Antecedentes y justificación: El objetivo de nuestro estudio fue estudiar la influencia de la variante rs16147 del gen NPY en la histología hepática en pacientes con enfermedad de hígado graso no alcohólico (NAFLD). Material y métodos: Se reclutó una muestra de 89 pacientes con NAFLD. Se realizó un análisis bioquímico del suero que incluyó perfil lipídico, transaminasas, adipoquinas y resistencia a la insulina. Se estudió el genotipo de polimorfismo (rs161477) del gen NPY. Resultados: Un total de 23 pacientes (25,0%) presentaron el genotipo GG (genotipo salvaje) y 66 pacientes (75%) GA (n=39) o AA (n=27) (genotipo mutante). Los pacientes con alelo A presentaron un menor porcentaje de inflamación lobulillar y esteatohepatitis que los pacientes con genotipo salvaje. Los pacientes con alelo A mostraron una puntuación más baja de SAF (esteatosis, actividad, fibrosis) que los no portadores de alelos A (5,4±2,7 puntos vs. 4,1±1,1 puntos, p=0,01). En el análisis realizado sin fibrosis (solo puntaje NAS), también se detectaron las mismas diferencias (4,5±1,8 puntos vs. 3,4±1,8 puntos, p=0,01). En el análisis de regresión logística a presencia del alelo A a una menor probabilidad de presentar con inflamación lobulillar (OR 0,11, IC 95%: 0,02-0,84; p=0,03) y esteatohepatitis (OR 0,39, IC 95%: 0,14-0,86, p=0,04) después de ajustar por edad, sexo e índice de masa corporal. Conclusiones: La variante del polimorfismo rs16147 del gen NPY se asocia de forma independiente con un menor porcentaje de esteatohepatitis e inflamación lobulillar en sujetos obesos con NAFLD diagnosticado con biopsia


Assuntos
Humanos , Masculino , Feminino , Adulto , Pessoa de Meia-Idade , Neuropeptídeo Y/genética , Neuropeptídeo Y/fisiologia , Obesidade/complicações , Obesidade/fisiopatologia , Hepatopatia Gordurosa não Alcoólica/etiologia , Hepatopatia Gordurosa não Alcoólica/fisiopatologia , Hepatopatia Gordurosa não Alcoólica/sangue , Polimorfismo Genético , Genótipo
16.
Nutr Hosp ; 36(1): 60-65, 2019 Mar 07.
Artigo em Inglês | MEDLINE | ID: mdl-30834760

RESUMO

Introduction: Background and objectives: the aim of the present investigation was to describe the association of this single nucleotide polymorphism (SNP) with fasting glucose levels, serum adipokine levels and diabetes mellitus. Methods: the study involved a population of 1,002 adult obese subjects. Measurements of anthropometric parameters, blood pressure, fasting blood glucose, C-reactive protein (CRP), insulin concentration, insulin resistance (HOMA-IR), HOMA-B, lipid profi le and adipocytokines levels were performed. Genotype of MTNR1B gene polymorphism (rs10830963) was evaluated. Results: fasting glucose levels (GG: 101.5 ± 19.1 mg/dl vs GT: 103.5 ± 8.1 units vs TT: 107.2 ± 8.0 mg/dl; p = 0.01) and HOMA-IR (GG: 3.1 ± 1.6 units vs GT: 3.4 ± 1.1 units vs TT: 3.7 ± 1.0 units; p = 0.02) were higher in subjects with GG genotype than in other genotypes. Total adiponectin levels (CC: 20.5 ± 8.4 ng/dl vs CG: 21.8 ± 5.4 ng/dl vs GG: 15.4 ± 1.4 ng/dl; p = 0.02) and HOMA-B (CC: 2.3 ± 0.8 units vs CG: 2.2 ± 1.1 units vs GG: 1.9 ± 0.9 units; p = 0.01) were lower in subjects with GG genotype than GC or CC genotypes. Logistic regression analysis showed an increased risk of hyperglicemia (OR = 1.31, 95% CI = 1.12-2.78, p = 0.03) and diabetes mellitus (OR = 1.37, 95% CI = 1.14-2.86, p = 0.04). Conclusions: this study showed that the MTNR1B rs10830963 polymorphism was associated with increased fasting glucose levels, HOMA-IR, and risk of DM2. This SNP was associated with decreased adiponectin levels and HOMA-B.


Assuntos
Adipocinas/sangue , Glicemia/análise , Síndrome Metabólica/sangue , Síndrome Metabólica/genética , Obesidade/sangue , Obesidade/genética , Polimorfismo Genético/genética , Receptor MT2 de Melatonina/genética , Adulto , Idoso , Antropometria , Pressão Sanguínea , Proteína C-Reativa/análise , Diabetes Mellitus Tipo 2/epidemiologia , Diabetes Mellitus Tipo 2/genética , Feminino , Genótipo , Humanos , Insulina/sangue , Resistência à Insulina , Lipídeos/sangue , Masculino , Pessoa de Meia-Idade
17.
Endocrinol. diabetes nutr. (Ed. impr.) ; 66(3): 157-163, mar. 2019. tab
Artigo em Espanhol | IBECS | ID: ibc-182615

RESUMO

Introducción: El papel de las variantes genéticas del gen CB2R en la pérdida de peso después de una intervención dietética se ha investigado en pocos estudios, y hasta la fecha en ninguno tras cirugía bariátrica. Objetivos: El objetivo del presente estudio fue evaluar el efecto de la variante genética (rs3123554) del gen CB2R sobre los factores de riesgo cardiovascular y la pérdida de peso secundaria a una derivación biliopancreática. Diseño: Se evaluó una muestra de 147 pacientes con obesidad mórbida. Los parámetros bioquímicos y antropométricos se estudiaron en la visita basal y en cada visita durante 3 años (1, 2 y 3 años). Resultados: El porcentaje de pérdida de peso en exceso, índice de masa corporal, peso, circunferencia de cintura, masa grasa, presión sanguínea, glucosa en ayunas, colesterol LDL, colesterol total, insulina, HOMA-IR y niveles de triglicéridos mejoraron en ambos genotipos tras la cirugía bariátrica. La disminución de los niveles de insulina en ayunas y HOMA-IR fue mayor en los portadores de alelos no A que en los portadores de alelos A. Conclusiones: Nuestros datos sugieren que los pacientes con obesidad mórbida sometidos a una derivación biliopancreática y que son portadores del alelo A de la variante rs3123554 del gen CB2R presentan más peso. Este alelo no influye en la pérdida de peso tras la cirugía, pero sí en una menor disminución de los niveles de insulina y resistencia a la insulina


Introduction: The role of genetic variants of the CB2R gene in weight loss after a dietary intervention has been investigated in few studies, none of which has been conducted after bariatric surgery. Objectives: The aim of this study was to assess the effect of the genetic variant (rs3123554) of the CB2R gene on cardiovascular risk factors and weight loss secondary to a biliopancreatic diversion. Design: The study simple consisted of 147 patients with morbid obesity. Biochemical and anthropometric parameters were measured at baseline and at each visit during 3 years (1, 2, and 3 years). Results: Percent excess weight loss, body mass index, weight, waist circumference, fat mass, blood pressure, fasting glucose, LDL cholesterol, total cholesterol, insulin, HOMA-IR, and triglyceride levels improved in both genotype groups. Decreases in fasting insulin levels and HOMA-IR were higher in non-A allele carriers as compared to A allele carriers. Conclusions: Our data suggest that patients with morbid obesity who undergo bariatric surgery and carry the A allele of variant rs3123554 of the CB2R gene have greater weight. This allele has no influence on weight loss after surgery, but results in a lower decrease in insulin levels and insulin resistance


Assuntos
Humanos , Masculino , Feminino , Adulto , Pessoa de Meia-Idade , Polimorfismo Genético , Receptores de Canabinoides/genética , Desvio Biliopancreático/métodos , Fatores de Risco , Perda de Peso/genética , Obesidade Mórbida/cirurgia , Obesidade Mórbida/genética , Antropometria , Índice de Massa Corporal
18.
Nutr. hosp ; 36(1): 60-65, ene.-feb. 2019. tab
Artigo em Inglês | IBECS | ID: ibc-183189

RESUMO

Background and objectives: the aim of the present investigation was to describe the association of this single nucleotide polymorphism (SNP) with fasting glucose levels, serum adipokine levels and diabetes mellitus. Methods: the study involved a population of 1,002 adult obese subjects. Measurements of anthropometric parameters, blood pressure, fasting blood glucose, C-reactive protein (CRP), insulin concentration, insulin resistance (HOMA-IR), HOMA-B, lipid profi le and adipocytokines levels were performed. Genotype of MTNR1B gene polymorphism (rs10830963) was evaluated. Results: fasting glucose levels (GG: 101.5 ± 19.1 mg/dl vs GT: 103.5 ± 8.1 units vs TT: 107.2 ± 8.0 mg/dl; p = 0.01) and HOMA-IR (GG: 3.1 ± 1.6 units vs GT: 3.4 ± 1.1 units vs TT: 3.7 ± 1.0 units; p = 0.02) were higher in subjects with GG genotype than in other genotypes. Total adiponectin levels (CC: 20.5 ± 8.4 ng/dl vs CG: 21.8 ± 5.4 ng/dl vs GG: 15.4 ± 1.4 ng/dl; p = 0.02) and HOMA-B (CC: 2.3 ± 0.8 units vs CG: 2.2 ± 1.1 units vs GG: 1.9 ± 0.9 units; p = 0.01) were lower in subjects with GG genotype than GC or CC genotypes. Logistic regression analysis showed an increased risk of hyperglicemia (OR = 1.31, 95% CI = 1.12-2.78, p = 0.03) and diabetes mellitus (OR = 1.37, 95% CI = 1.14-2.86, p = 0.04). Conclusions: this study showed that the MTNR1B rs10830963 polymorphism was associated with increased fasting glucose levels, HOMA-IR, and risk of DM2. This SNP was associated with decreased adiponectin levels and HOMA-B.


Introducción y objetivos: el objetivo de la presente investigación fue describir la asociación del polimorfi smo del gen MTNR1B (rs10830963) con los niveles de glucosa en ayunas, los niveles séricos de adipocitoquinas y la diabetes mellitus. Métodos: el estudio incluyó una población de 1.002 adultos obesos. Se realizó la determinación de parámetros antropométricos, presión arterial, glucosa en sangre en ayunas, proteína C reactiva (CRP), concentración de insulina, resistencia a la insulina (HOMA-IR), HOMA-B, perfi l lipídico y niveles de adipocitoquinas. Se evaluó el genotipo del polimorfi smo del gen MTNR1B (rs10830963). Resultados: los niveles de glucosa en ayunas (GG: 101,5 ± 19,1 mg/dl vs. GT: 103,5 ± 8,1 unidades vs. TT: 107,2 ± 8,0 mg/dl; p = 0,01) y HOMA-IR (GG: 3,1 ± 1,6 unidades vs. GT: 3,4 ± 1,1 unidades vs. TT: 3,7 ± 1,0 unidades, p = 0,02) fueron más altos en sujetos con genotipo GG que en otros genotipos. Los niveles totales de adiponectina (CC: 20,5 ± 8,4 ng/dl vs. CG: 21,8 ± 5,4 ng/dl vs. GG: 15,4 ± 1,4 ng/dl, p = 0,02) y HOMA-B (CC: 2,3 ± 0,8 unidades vs. CG: 2,2 ± 1,1 unidades frente a GG: 1,9 ± 0,9 unidades, p = 0,01) fueron menores en sujetos con genotipo GG que en genotipos GC o CC. El análisis de regresión logística mostró un mayor riesgo de hiperglicemia (OR = 1,31, IC 95% = 1,12-2,78, p = 0,03) y diabetes mellitus (OR = 1,37, IC 95% = 1,14-2,86, p = 0,04). Conclusiones: este estudio mostró que el polimorfi smo MTNR1B rs10830963 se asoció con un aumento de los niveles de glucosa en ayunas, HOMA-IR y riesgo de DM2. Este SNP se asoció con niveles de adiponectina disminuidos y HOMA-B


Assuntos
Humanos , Masculino , Feminino , Adulto , Pessoa de Meia-Idade , Idoso , Adipocinas/sangue , Glicemia/análise , Síndrome Metabólica/sangue , Síndrome Metabólica/genética , Obesidade/sangue , Obesidade/genética , Polimorfismo Genético , Receptor MT2 de Melatonina/genética , Antropometria , Pressão Sanguínea , Proteína C-Reativa/análise , Diabetes Mellitus Tipo 2/epidemiologia , Diabetes Mellitus Tipo 2/genética , Genótipo , Insulina/sangue , Resistência à Insulina , Lipídeos/sangue
19.
Endocrinol Diabetes Nutr ; 66(4): 217-222, 2019 Apr.
Artigo em Inglês, Espanhol | MEDLINE | ID: mdl-30691985

RESUMO

BACKGROUND AND RATIONALE: This study was intended to assess the influence of the rs16147 variant of the NPY gene on liver histology in patients with non-alcoholic fatty liver disease (NAFLD). MATERIAL AND METHODS: Eighty-nine patients with NAFLD were recruited into the study. Serum chemistry tests were done including lipid profile, transaminases, adipokines, and insulin resistance. Genotype of polymorphism (rs161477) of the NPY gene was studied. RESULTS: Twenty-three patients (25.0%) had the GG genotype (wild type) and sixty-six patients (75%) the GA (n=39) or AA (n=27) (mutant) types. Patients with A allele had a lower percentage of lobular inflammation and steatohepatitis (lobular inflammation plus ballooning) than those with wild genotype. Patients with A allele showed lower SAF (Steatosis, Activity, Fibrosis) scores than non-A allele carriers (5.4±2.7 points vs. 4.1±1.1 points; p=0.01). In the analysis without fibrosis (NAS score), the same differences were detected (4.5±1.8 points vs. 3.4±1.8 points; p=0.01). In the logistic regression analysis A allele carriers showed lower odds for inflammation (OR 0.11, 95% CI 0.02-0.84, p=0.03) and steatohepatitis (OR 0.39, 95% CI 0.14-0.86, p=0.04) after adjusting for age, sex, and body mass index. CONCLUSIONS: A variant of polymorphism rs16147 of the NPY gene is independently associated to a lower percentage of steatohepatitis and lobular inflammation in obese subjects with biopsy-proven NAFLD.


Assuntos
Neuropeptídeo Y/genética , Hepatopatia Gordurosa não Alcoólica/genética , Obesidade/genética , Polimorfismo de Nucleotídeo Único , Adipocinas/sangue , Adiposidade , Adulto , Glicemia/análise , Peso Corporal , Comorbidade , Feminino , Estudos de Associação Genética , Genótipo , Humanos , Resistência à Insulina , Lipídeos/sangue , Fígado/patologia , Masculino , Pessoa de Meia-Idade , Hepatopatia Gordurosa não Alcoólica/sangue , Hepatopatia Gordurosa não Alcoólica/epidemiologia , Hepatopatia Gordurosa não Alcoólica/patologia , Obesidade/epidemiologia
20.
J Diabetes Complications ; 33(3): 249-254, 2019 03.
Artigo em Inglês | MEDLINE | ID: mdl-30467071

RESUMO

BACKGROUND & AIMS: A common G-to-A transition located 75 base pairs upstream (rs670) from transcription start site of the APOA1 gene has been related with some metabolic parameters. Our aim was to analyze the effects of rs670 APOA1 gene polymorphism on lipid profile and metabolic changes after two different hypocaloric diets. METHODS: 282 obese subjects were randomly allocated during 12 weeks (Diet HF - high fat diet vs. Diet LF - low fat diet). Anthropometric and biochemical status were evaluated. RESULTS: Body mass index, weight, fat mass, waist circumference, systolic blood pressure, leptin levels and waist circumference decreased in all patients in average after both diets. In A allele carriers after 12 weeks with both diets, insulin levels (Delta diet HF: -5.3 + 1.2 UI/L; P = 0.02 and Delta diet LF: -5.8 + 1.3 UI/L; P = 0.02) and HOMA-IR (Delta diet HF: -2.9 + 0.8 units; P = 0.01 and Delta diet LF: -2.2 + 0.9 units; P = 0.03) improved in a significant way. With the low fat diet, A allele carriers showed a statistical improvement in HDL-cholesterol levels (Delta: 4 + 1 mg/dl; P = 0.03). CONCLUSIONS: Our study showed the association of rs670 ApoA1 polymorphism with a decrease of insulin resistance induced by both diets and provided additional evidence on HDL-cholesterol increase after a LF hypocaloric diet in A allele carriers.

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