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1.
Molecules ; 26(11)2021 Jun 07.
Artigo em Inglês | MEDLINE | ID: mdl-34200200

RESUMO

Epilobium angustifolium L. is a popular and well-known medicinal plant. In this study, an attempt to evaluate the possibility of using this plant in preparations for the care and treatment of skin diseases was made. The antioxidant, antiaging and anti-inflammatory properties of ethanolic extracts from Epilobium angustifolium (FEE) were assessed. Qualitative and quantitative evaluation of extracts chemically composition was performed by gas chromatography with mass spectrometry (GC-MS) and high-performance liquid chromatography (HPLC). The total polyphenol content (TPC) of biologically active compounds, such as the total content of polyphenols (TPC), flavonoids (TFC), and assimilation pigments, as well as selected phenolic acids, was assessed. FEE was evaluated for their anti-inflammatory and antiaging properties, achieving 68% inhibition of lipoxygenase activity, 60% of collagenase and 49% of elastase. FEE also showed high antioxidant activity, reaching to 87% of free radical scavenging using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 59% using 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS). Additionally, in vitro penetration studies were performed using two vehicles, i.e., a hydrogel and an emulsion containing FEE. These studies showed that the active ingredients contained in FEE penetrate through human skin and accumulate in it. The obtained results indicate that E. angustifolium may be an interesting plant material to be applied as a component of cosmetic and dermatological preparations with antiaging and anti-inflammatory properties.


Assuntos
Cosméticos/química , Fármacos Dermatológicos/química , Epilobium/química , Extratos Vegetais/química , Anti-Inflamatórios/química , Antioxidantes/química , Compostos de Bifenilo/química , Cromatografia Líquida de Alta Pressão/métodos , Flavonoides/química , Cromatografia Gasosa-Espectrometria de Massas/métodos , Humanos , Plantas Medicinais/química , Polifenóis/química , Pele/efeitos dos fármacos
2.
Biomolecules ; 11(7)2021 06 24.
Artigo em Inglês | MEDLINE | ID: mdl-34202667

RESUMO

Increased triacylglycerols' (TAG) synthesis, insulin resistance, and prolonged liver lipid storage might lead to the development of non-alcoholic fatty liver disease (NAFLD). Global prevalence of NAFLD has been estimated to be around 25%, with gradual elevation of this ratio along with the increased content of adipose tissue in a body. The initial stages of NAFLD may be reversible, but the exposition to pathological factors should be limited. As dietary factors greatly influence various disease development, scientists try to find dietary components, helping to alleviate the steatosis. These components include n-3 polyunsaturated (PUFA) fatty acids, especially eicosapentaenoic acid (EPA) and docosahexaenoic acids (DHA). This review focused on the role of resolvins, protectins and merensins in NAFLD.


Assuntos
Ácidos Docosa-Hexaenoicos/administração & dosagem , Ácido Eicosapentaenoico/administração & dosagem , Ácidos Graxos Ômega-3/administração & dosagem , Hepatopatia Gordurosa não Alcoólica/dietoterapia , Hepatopatia Gordurosa não Alcoólica/metabolismo , Animais , Antígenos CD59/administração & dosagem , Dieta/efeitos adversos , Humanos , Resistência à Insulina/fisiologia , Hepatopatia Gordurosa não Alcoólica/etiologia
3.
Int J Med Sci ; 18(6): 1519-1531, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33628110

RESUMO

Chronological skin ageing is an inevitable physiological process that results in thin and sagging skin, fine wrinkles, and gradual dermal atrophy. The main therapeutic approaches to soft tissue augmentation involve using dermal fillers, where natural fillers, such as autologous fibroblasts, are involved in generating dermal matrix proteins. The aim of this study was to determine the global transcriptome profile of three passages of dermal autologous fibroblasts from a male volunteer, focusing on the processes of the cell cycle and cell proliferation status to estimate the optimal passage of the tested cells with respect to their reimplantation. We performed K-means clustering and validation of the expression of the selected mRNA by qRT-PCR. Ten genes were selected (ANLN, BUB1, CDC20, CCNA2, DLGAP5, MKI67, PLK1, PRC1, SPAG5, and TPX2) from the top five processes annotated to cluster 5. Detailed microarray analysis of the fibroblast genes indicated that the cell population of the third passage exhibited the highest number of upregulated genes involved in the cell cycle and cell proliferation. In all cases, the results of qRT-PCR confirmed the differences in expression of the selected mRNAs between fibroblasts from the primary culture (C0) and from the first (C1), second (C2), and third (C3) cell passage. Our results thus suggest that these cells might be useful for increasing fibroblast numbers after reimplantation into a recipient's skin, and the method used in this study seems to be an excellent tool for autologous transplantation allowing the rejuvenation of aging skin.


Assuntos
Técnicas Cosméticas , Fibroblastos/fisiologia , Envelhecimento da Pele/genética , Pele/citologia , Ciclo Celular/genética , Proliferação de Células/genética , Células Cultivadas , Face , Fibroblastos/transplante , Perfilação da Expressão Gênica , Regulação da Expressão Gênica/fisiologia , Humanos , Masculino , Pessoa de Meia-Idade , Cultura Primária de Células , Reação em Cadeia da Polimerase em Tempo Real , Rejuvenescimento , Transplante Autólogo/métodos
4.
Molecules ; 26(2)2021 Jan 10.
Artigo em Inglês | MEDLINE | ID: mdl-33435259

RESUMO

Epilobium angustifolium L. is applied as an antiseptic agent in the treatment of skin diseases. However, there is a lack of information on human skin penetration of active ingredients with antioxidative potential. It seems crucial because bacterial infections of skin and subcutaneous tissue are common and partly depend on oxidative stress. Therefore, we evaluated in vitro human skin penetration of fireweed ethanol-water extracts (FEEs) by determining antioxidant activity of these extracts before and after penetration study using 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), and Folin-Ciocalteu methods. Microbiological tests of extracts were done. The qualitative and quantitative evaluation was performed using gas chromatography-mass spectrometry (GC-MS) and high-performance liquid chromatography (HPLC-UV) methods. The in vitro human skin penetration using the Franz diffusion chamber was assessed. The high antioxidant activity of FEEs was found. Gallic acid (GA), chlorogenic acid (ChA), 3,4-dihydroxybenzoic acid (3,4-DHB), 4-hydroxybenzoic acid (4-HB), and caffeic acid (CA) were identified in the extracts. The antibacterial activities were found against Serratia lutea, S. marcescens, Bacillus subtilis, B. pseudomycoides, and B. thuringiensis and next Enterococcus faecalis, E. faecium, Streptococcus pneumoniae, Pseudomonas aeruginosa, and P. fluorescens strains. In vitro penetration studies showed the penetration of some phenolic acids and their accumulation in the skin. Our results confirm the importance of skin penetration studies to guarantee the efficacy of formulations containing E. angustifolium extracts.


Assuntos
Anti-Infecciosos , Antioxidantes , Bactérias/crescimento & desenvolvimento , Bassia scoparia/química , Extratos Vegetais , Absorção Cutânea , Pele/metabolismo , Anti-Infecciosos/química , Anti-Infecciosos/farmacocinética , Anti-Infecciosos/farmacologia , Antioxidantes/química , Antioxidantes/farmacocinética , Antioxidantes/farmacologia , Etanol/química , Humanos , Extratos Vegetais/química , Extratos Vegetais/farmacocinética , Extratos Vegetais/farmacologia , Pele/microbiologia , Água/química
5.
Int J Mol Sci ; 21(20)2020 Oct 15.
Artigo em Inglês | MEDLINE | ID: mdl-33076354

RESUMO

INTRODUCTION: Most ischemic strokes develop as a result of atherosclerosis, in which inflammation plays a key role. The synthesis cascade of proinflammatory mediators participates in the process induced in the vascular endothelium and platelets. Resolvins are anti-inflammatory mediators originating from eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), which may improve the prognosis related to atherosclerosis by inhibiting the production of proinflammatory cytokines, limiting neutrophil migration, or positively influencing phagocytosis. Although clinical trials with resolvin in humans after stroke have not been realized, they may soon find application. AIM: The aim of the study was to review the available literature on the scope of the possibilities of the prevention and treatment of stroke with the use of resolvins, EPA and DHA derivatives. MATERIALS AND METHODS: The review features articles published until 31 January 2020. The search for adequate literature was conducted using the keywords: stroke and resolvins. Over 150 articles were found. Studies not written in English, letters to the editor, conference abstracts, and duplicate information were excluded. RESULTS: In several studies using the animal model, the supplementation of resolvin D2 decreased brain damage caused by myocardial infarction, and it reversed the neurological dysfunction of the brain. A decrease in the concentration of proinflammatory cytokines, such as TNF-α, Il-6, and Il-1ß, was also observed, as well as a decrease in the scope of brain damage. In the context of stroke in animals, the treatment with resolvin D2 (RvD2) (injection) has a better effect than supplementation with DHA. CONCLUSIONS: Resolvins are characterised by strong anti-inflammatory properties. Resolvins improve prognosis and decrease the risk of developing cardiovascular disease, consequently lowering the risk of stroke, and may find application in the treatment of stroke.


Assuntos
Ácidos Docosa-Hexaenoicos/metabolismo , Ácido Eicosapentaenoico/metabolismo , AVC Isquêmico/metabolismo , Animais , Citocinas/metabolismo , Ácidos Docosa-Hexaenoicos/análogos & derivados , Ácidos Docosa-Hexaenoicos/uso terapêutico , Ácido Eicosapentaenoico/análogos & derivados , Ácido Eicosapentaenoico/uso terapêutico , Humanos , AVC Isquêmico/tratamento farmacológico , Receptores Acoplados a Proteínas G/metabolismo
6.
Postepy Dermatol Alergol ; 37(2): 269-271, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-32489365

RESUMO

Introduction: The constantly rising number of skin malignancies and increasing cancer awareness encourage more people to visit outpatient clinics in order to have various skin lesions removed. Despite the fact that scarring is a physiological response to any excision procedure, minimizing the size of it is a goal of every good practitioner. Therefore the question arises whether different techniques used to remove skin lesions may impact the formation and quality of skin scars. Aim: To perform an evaluation of skin scars formed by laser and surgical incisions and their influence on lymphatic outflow in rats. Material and methods: Five male rats were used. Using methylene blue, the migration of dye through lymphatic channels of the lower extremity was measured. Afterwards, transverse incisions were made distally using laser and a surgical blade. Wounds were left to heal by secondary intention. After 4 weeks dye migration assessment was repeated and tissue samples were obtained for microscopic evaluation. Results: Wounds after surgical incisions healed entirely. Wounds after laser treatment had not healed, with a visible area of granulation tissue and hair loss. Significantly worse dye migration was observed in rat extremities after laser therapy than after surgical incision (p = 0.007). Conclusions: The results of the study show that the size of the scar can depend on the incision technique used. Larger scars after laser therapy limit the lymphatic flow of the skin, which may have an adverse effect on mapping sentinel lymph nodes. However, this hypothesis requires further research.

7.
ACS Omega ; 4(22): 19765-19771, 2019 Nov 26.
Artigo em Inglês | MEDLINE | ID: mdl-31788608

RESUMO

The healing process of the fractured bone in a presence of poly(butylene succinate-butylene dilinoleate) (PBS-DLA) copolymer containing nanosized hydroxyapatite (HAP) particles has been investigated. The PBS-DLA material containing PBS hard segments and DLA soft segments (50:50 wt %) was used to prepare a polymer/ceramic composite with 30 wt % HAP. A new PBS-DLA copolymer showed a high elasticity of 500% and 15 MPa tensile strength. Addition of HAP improved tensile strength up to 25 MPa while high elasticity has been preserved going down only to 300% of elongation at break. A polymer nanocomposite was fabricated into small elastic polymer rods 15 mm long and 1 × 2 mm in cross section and used for tibia bone fixation in rats. Mallory trichrome staining indicated that new biodegradable copolymers and its composite containing HAP have triggered the most advanced bone healing of all tested materials, thus indicating their high potential for bone tissue engineering and repair.

8.
Wideochir Inne Tech Maloinwazyjne ; 9(3): 319-28, 2014 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-25337153

RESUMO

INTRODUCTION: Methods allowing one to locate the position of a cutaneous perforator do not allow one to determine the boundaries of the vascularized skin. In clinical practice this causes complications in the form of marginal necrosis of the flap. AIM: To examine the usefulness of thermography to assess the extent of vascularization of the skin and subcutaneous tissue by a single perforator. MATERIAL AND METHODS: Thirty-one male rats were used. Using dynamic thermography the perforators on the abdominal skin were located. Afterwards the flap was prepared on a randomly chosen perforator. After 24 h the extent of vascularization of the skin by a single perforator was examined. RESULTS: In 22.5% of cases the number of perforators marked in the thermography was equal to the number of perforators marked intraoperatively, in 64.5% it was lower and in 13% higher. The use of thermography has shown that basing the flap vascularization on the perforator with low efficiency resulted in statistically more frequent occurrence of ischemia and partial necrosis of the flap (p = 0.024). Partial necrosis of the flap occurred in 12 of 31 cases, always in the area in which during the preoperative thermography no perforators were found. The areas of necrosis occurred irrespectively of the distance from the supplying vessel. CONCLUSIONS: When designing the shape of the flap, the distribution of all perforators must be considered. The perforators need to be included in the area of prepared tissues because their location indicates the area with a more efficient network of vessels.

9.
Wideochir Inne Tech Maloinwazyjne ; 9(3): 329-36, 2014 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-25337154

RESUMO

INTRODUCTION: Tissue perfusion disorders can be present in various diseases and progress in the form of arterial ischemia or venous stasis with accompanying local changes in temperature. AIM: To use of thermography in the diagnostics of early periods of tissue perfusion disorders before the clinical symptoms occur. MATERIAL AND METHODS: Thirty-two male rats were used. After anesthesia the skin on lower limbs was shaved and femoral vessels of both sides were exposed. In 10 rats the left femoral artery was ligated, in 12 rats the left femoral vein was ligated and in the 10 remaining rats both left femoral vessels were ligated. Thermography of the limbs was performed before the vessels were ligated and after a period of 24 h. The pictures were taken every 5 s during 3 min. Before the measurement, the tissues were cooled down for 20 s with a 5°C water compress. The rate of temperature return to the limbs was evaluated. RESULTS: Statistically significant differences were observed after the 24-hour period on the thigh after the ligation of the vein, and on the shank and the foot after ligation of the artery. After the ligature of both vessels, statistically significant differences occurred immediately after their ligature within the thigh and shank and after 24 h on the foot. CONCLUSIONS: The results show that cameras with an accuracy of 0.05°C can be used to detect tissue perfusion disorders. The special diagnostic value is the ability to detect perfusion disorders before clinical symptoms occur.

10.
J Transl Med ; 11: 11, 2013 Jan 09.
Artigo em Inglês | MEDLINE | ID: mdl-23302473

RESUMO

BACKGROUND: It has been suggested that action of complement cascade [CC]-derived anaphylatoxins/molecules may represent a missing link between obesity and metabolic disorders. However, to date, the direct biochemical/immunomodulatory composition of the human AT environment remains poorly understood. In this study, we examined plasma and AT (subcutaneous and visceral/omental) levels of selected CC-derived anaphylatoxins/molecules, and adipsin as well as verified their associations with immune and stem cells chemoattractant - stromal-derived factor-1 (SDF-1). METHODS: A total of 70 (35 subcutaneous and 35 omental) AT samples were obtained from patients undergoing elective surgery. Plasma and AT-derived interstitial fluid levels of C3a, C5a, C5b-9/membrane attack complex (MAC), complement factor D (adipsin) were measured using ELISA. RESULTS: AT levels of all examined substances were significantly lower than the corresponding levels in the plasma (in all cases P < 0.0000001). Moreover, in subcutaneous AT, robust C3a and adipsin concentrations were observed, whereas high levels of C5b-9/MAC were detected in the visceral depots. In addition, we established the correlations between analyzed molecular substances and body composition, BMI and/or the adiposity index of the examined patients. CONCLUSIONS: Our study demonstrated for the first time that significantly reduced levels of complement-derived molecules were present in human AT than in the peripheral blood, and that these factors are associated with the metabolic status of examined individuals. Moreover, in human AT, various associations between complement-derived molecules and SDF-1 levels exist.


Assuntos
Tecido Adiposo/metabolismo , Proteínas do Sistema Complemento/fisiologia , Adulto , Estudos de Casos e Controles , Fator D do Complemento/metabolismo , Proteínas do Sistema Complemento/metabolismo , Líquido Extracelular/metabolismo , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Obesidade/metabolismo , Sobrepeso/metabolismo
11.
Wiad Lek ; 64(1): 15-21, 2011.
Artigo em Polonês | MEDLINE | ID: mdl-21812358

RESUMO

"Heart-hand" syndrome is a broad category of diseases. The most common form is Holt-Oram syndrome (HOS) that occurs in approximately 1:100 000 live births. It is characterized by upper limb defects (carpal bone defects, triphalangeal thumbs, hypoplasia or absence of the thumb and the radial ray) and cardiac septal defects (atrial septal defects or ventricular septal defects). There are three main types of "heart-hand" syndromes. "Heart-hand" syndrome type I--HOS is characterised by atrial septal defect and thumb anomaly, type II (Tabatznik syndrome) by short distal phalanx of the thumb, upper limb abnormalities and cardiac arrhythmias, type III by cardiac conduction diseases and shortening of the middle phalanges. The aim of this report is to present a new case of the "heart-hand" syndrome in the family. This diagnosis was established on the base of clinical examination, radiological findings, and echocardiography. Our patient demonstrates congenital bilateral absence of a radial bone and thumbs, dextrocardia and patent foramen ovale.


Assuntos
Anormalidades Múltiplas/diagnóstico , Cardiopatias Congênitas/diagnóstico , Comunicação Interatrial/diagnóstico , Deformidades Congênitas das Extremidades Inferiores/diagnóstico , Deformidades Congênitas das Extremidades Superiores/diagnóstico , Criança , Pré-Escolar , Humanos , Masculino
12.
Biomaterials ; 31(9): 2477-88, 2010 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-20034664

RESUMO

This paper presents the synthesis and characterization of a polyisobutylene (PIB)-based nanostructured carbon-reinforced thermoplastic elastomer. This thermoplastic elastomer is based on a self-assembling block copolymer having a branched PIB core carrying -OH functional groups at each branch point, flanked by blocks of poly(isobutylene-co-para-methylstyrene). The block copolymer has thermolabile physical crosslinks and can be processed as a plastic, yet retains its rubbery properties at room temperature. The carbon-reinforced thermoplastic elastomer had more than twice the tensile strength of the neat polymer, exceeding the strength of medical grade silicone rubber, while remaining significantly softer. The carbon-reinforced thermoplastic elastomer displayed a high T(g) of 126 degrees C, rendering the material steam-sterilizable. The carbon also acted as a free radical trap, increasing the onset temperature of thermal decomposition in the neat polymer from 256.6 degrees C to 327.7 degrees C. The carbon-reinforced thermoplastic elastomer had the lowest water contact angle at 82 degrees and surface nano-topography. After 180 days of implantation into rabbit soft tissues, the carbon-reinforced thermoplastic elastomer had the thinnest tissue capsule around the microdumbbell specimens, with no eosinophiles present. The material also showed excellent integration into bones.


Assuntos
Carbono/farmacologia , Elastômeros/farmacologia , Nanoestruturas/química , Plásticos/farmacologia , Polienos/farmacologia , Polímeros/farmacologia , Temperatura , Animais , Osso e Ossos/citologia , Osso e Ossos/efeitos dos fármacos , Morte Celular/efeitos dos fármacos , Hidrólise/efeitos dos fármacos , Implantes Experimentais , Espectroscopia de Ressonância Magnética , Teste de Materiais , Fenômenos Mecânicos/efeitos dos fármacos , Microscopia de Força Atômica , Músculos/citologia , Músculos/efeitos dos fármacos , Implantação de Prótese , Coelhos , Estresse Mecânico , Propriedades de Superfície/efeitos dos fármacos , Termogravimetria , Água/química
13.
Biomacromolecules ; 7(3): 844-50, 2006 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-16529422

RESUMO

This paper will discuss the biocompatibility and dynamic fatigue properties of polystyrene-b-polyisobutylene-b-polystyrene thermoplastic elastomer with 30 wt % polystyrene (SIBS30), an emerging FDA-approved biomaterial. SIBS30 is a very soft, transparent biomaterial resembling silicone rubber, with superior mechanical properties. Using the hysteresis method adopted for soft biomaterials, the dynamic fatigue properties of SIBS30 were found to be between those of polyurethane and silicone rubber, with fatigue life twice as long as that of silicone. Under single load testing (SLT, 1.25 MPa), SIBS30 displayed less than half the dynamic creep compared to silicone, both in air and in vitro (37 degrees C, simulated body fluid). Hemolysis and 30- and 180-day implantation studies revealed excellent biocompatibility of the new biomaterial. The results presented in this paper indicate that, in comparison with silicone rubber, SIBS30 has similar biocompatibility and superior dynamic fatigue properties.


Assuntos
Materiais Biocompatíveis/química , Teste de Materiais/instrumentação , Estirenos/química , Animais , Elasticidade , Hemólise , Temperatura Alta , Humanos , Modelos Químicos , Modelos Moleculares , Ovinos , Silicones/química , Resistência à Tração
14.
Chir Narzadow Ruchu Ortop Pol ; 69(1): 15-7, 2004.
Artigo em Polonês | MEDLINE | ID: mdl-15305667

RESUMO

During 1995-2002 in General and Hand Surgery Department 21 digits were reconstructed by toe to hand transplantation performed in 16 patients. There were 10 cases thumb was reconstruction with 4 great toe and 6 second toe transfers. Rest 11 fingers there were index, middle and ring were restored by II and III toe. Twelve osteosynthesis were performed by Kirschner wire and nine by bone peg. All fixations were sufficient stable to start early rehabilitation. In one case after Kirschner fixation occurred ostitis and malunion. This patient was cured by autovaccine. After bone peg we do not recorded any complication.


Assuntos
Pinos Ortopédicos , Fios Ortopédicos , Traumatismos dos Dedos/cirurgia , Fixação Intramedular de Fraturas/instrumentação , Dedos do Pé/transplante , Adolescente , Adulto , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Complicações Pós-Operatórias , Resultado do Tratamento
15.
Biomaterials ; 23(14): 2973-8, 2002 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-12069339

RESUMO

This study was undertaken to evaluate the biocompatibility of new multiblock poly(ester-ester)s proposed as an alternative to Hunter silastic prosthesis used in a two-stage tendon reconstruction. Methanol-extracted polymeric material retained its weight, demonstrating the absence of leachable particles (e.g. low-molecular weight oligomers). Implantation tests indicated that the observed tissue changes were similar to those obtained with silicone, no evidence of contact necrosis being observed. The unchanged morphology of rat liver hepatocytes and the lack of parenchymal necrosis also indicated that exposure to the investigated polymers did not cause any cytotoxic reactions.


Assuntos
Materiais Biocompatíveis/química , Ácidos Graxos/química , Poliésteres/química , Próteses e Implantes , Tendões/cirurgia , Animais , Tecido Conjuntivo , Dimerização , Fígado/citologia , Linfócitos/citologia , Teste de Materiais , Coelhos , Ratos , Ratos Wistar , Procedimentos Cirúrgicos Reconstrutivos , Fatores de Tempo
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