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1.
Braz. dent. j ; 33(3): 82-91, July-Sept. 2022. graf
Artigo em Inglês | LILACS-Express | LILACS, BBO - Odontologia | ID: biblio-1384027

RESUMO

Abstract The aim of this study was to evaluate a Demineralized Human Dentine Matrix (DHDM) as viable biomaterial for alveolar ridge preservation in a rat model. Wistar rats were submitted to the extraction of maxillary first molars bilaterally. Sockets were filled with biomaterials and divided into 4 experimental groups (n=5): blood clot, autogenous bone, bovine-derived xenograft (BDX) and DHDM. Animals were sacrificed at 7, 14 e 28 days. Microtomography (uCT) volumetric evaluation and qualitative histological analyses were performed. Results obtained through the uCT showed similar values between the DHDM and the other experimental groups. The histological evaluation demonstrated DHDM with an unspecific inflammatory process and bone neoformation with slow reabsorption of the material. This result indicates that DHDM implanted in rat sockets is biocompatible and reduces the alveolar ridge volume loss after tooth extraction.


Resumo O objetivo deste estudo foi avaliar a Matriz Dentinária Humana Desmineralizada (MDHD) como biomaterial viável para preservação do rebordo alveolar, no modelo em rato. Ratos Wistar foram submetidos à exodontias dos primeiros molares superiores bilateralmente. Os alvéolos foram preenchidos com biomateriais e divididos em 4 grupos experimentais (n=5): coágulo sanguíneo, osso autógeno, osso xenógeno de origem bovina e MDHD. Os animais foram sacrificados aos 7, 14 e 28 dias. Foram realizadas avaliações volumétricas por microtomografia (uCT) e análises histológicas qualitativas. Os resultados obtidos por meio do uCT mostraram valores semelhantes entre o MDHD e os demais grupos experimentais. A avaliação histológica demonstrou MDHD com processo inflamatório inespecífico e neoformação óssea com lenta reabsorção do material. Esse resultado indica que a MDHD implantada em alvéolo de rato é biocompatível e reduz a perda de volume do rebordo alveolar após extração dentária.

2.
Braz Dent J ; 33(3): 82-91, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35766721

RESUMO

The aim of this study was to evaluate a Demineralized Human Dentine Matrix (DHDM) as viable biomaterial for alveolar ridge preservation in a rat model. Wistar rats were submitted to the extraction of maxillary first molars bilaterally. Sockets were filled with biomaterials and divided into 4 experimental groups (n=5): blood clot, autogenous bone, bovine-derived xenograft (BDX) and DHDM. Animals were sacrificed at 7, 14 e 28 days. Microtomography (uCT) volumetric evaluation and qualitative histological analyses were performed. Results obtained through the uCT showed similar values between the DHDM and the other experimental groups. The histological evaluation demonstrated DHDM with an unspecific inflammatory process and bone neoformation with slow reabsorption of the material. This result indicates that DHDM implanted in rat sockets is biocompatible and reduces the alveolar ridge volume loss after tooth extraction.


Assuntos
Perda do Osso Alveolar , Alvéolo Dental , Processo Alveolar , Animais , Bovinos , Dentina , Humanos , Ratos , Ratos Wistar , Extração Dentária , Alvéolo Dental/cirurgia
3.
Materials (Basel) ; 14(24)2021 Dec 08.
Artigo em Inglês | MEDLINE | ID: mdl-34947112

RESUMO

In this study, the preparation and characterization of three hydroxyapatite-based bioactive scaffolds, including hydroxyapatite microspheres (HAps), amoxicillin-hydroxyapatite composite (Amx-HAp), and collagen-hydroxyapatite composite (Col-HAp) were performed. In addition, their behavior in human dental pulp mesenchymal stem cell (hDPSC) culture was investigated. HAps were synthesized through the following methods: microwave hydrothermal, hydrothermal reactor, and precipitation, respectively. hDPSCs were obtained from samples of third molars and characterized by immunophenotypic analysis. Cells were cultured on scaffolds with osteogenic differentiation medium and maintained for 21 days. Cytotoxicity analysis and migration assay of hDPSCs were evaluated. After 21 days of induction, no differences in genes expression were observed. hDPSCs highly expressed the collagen IA and the osteonectin at the mRNA. The cytotoxicity assay using hDPSCs demonstrated that the Col-HAp group presented non-viable cells statistically lower than the control group (p = 0.03). In the migration assay, after 24 h HAps revealed the same migration behavior for hDPSCs observed compared to the positive control. Col-HAp also provided a statistically significant higher migration of hDPSCs than HAps (p = 0.02). Migration results after 48 h for HAps was intermediate from those achieved by the control groups. There was no statistical difference between the positive control and Col-HAp. Specifically, this study demonstrated that hydroxyapatite-based bioactive scaffolds, especially Col-Hap, enhanced the dynamic parameters of cell viability and cell migration capacities for hDPSCs, resulting in suitable adhesion, proliferation, and differentiation of this osteogenic lineage. These data presented are of high clinical importance and hold promise for application in therapeutic areas, because Col-HAp can be used in ridge preservation, minor bone augmentation, and periodontal regeneration. The development of novel hydroxyapatite-based bioactive scaffolds with clinical safety for bone formation from hDPSCs is an important yet challenging task both in biomaterials and cell biology.

4.
Rev. ABENO ; 21(1): 1255, dez. 2021. tab, graf
Artigo em Português | LILACS, BBO - Odontologia | ID: biblio-1371185

RESUMO

Oobjetivo desteestudofoianalisaro perfil dos usuários do Biobanco de Dentes Humanos da Universidade Federal do Paraná (BDH-UFPR), os serviços mais procurados, se háutilização de dentes obtidos externamentee o grau de satisfaçãodos usuários.Para isso,foi aplicado um questionário eletrônico semiestruturado à comunidade interna do curso de Odontologia daUFPR, entre agosto edezembro de 2019. Um total de 300 questionários foram respondidos, sendo263 por estudantes degraduação, 4 por pós-graduandos, 24 por professores e 9 por servidores técnico-administrativos.A maioria dos estudantes erado sexo feminino entre 17e 22 anos, enquantopara osservidores (professores e técnicos) houve uma distribuição semelhante entre os sexosna faixa etária entre 40e 59 anos. Embora 80,99% dos discentes afirmaram conhecer oBDH-UFPR, apenas 50,19%usaram seus serviçose59,32% relataram játerembuscadodentesexternamente.O serviço mais utilizado foi o empréstimo de dentes para atividades de ensino, porém apenas 42,15% os devolveram após ouso.Os dentes obtidos externamente foram provenientes,principalmente, deconsultórios particulares e apenas 56,33% dos alunosrelataram sempre ter submetido-os à desinfecção/esterilização, sendo a autoclave o método mais indicado. Todos os servidores conheciam o BDH-UFPR e,dentre os professores,a maioria realizoutanto empréstimos quanto doações. Os empréstimostiveram como principal finalidade a pesquisae 63,6% relataramterem devolvido os dentes.Quanto à satisfação, a comunidade acadêmica mostrou-se predominantemente totalmente satisfeita.Por fim, ressalta-se a necessidade de ampliar as ações de conscientização para a comunidade acadêmica quanto aos aspectos éticos, legais e de biossegurança na aquisição e manipulação de dentes humanos (AU).


Thisstudy aimed toanalyze the profile of users of the Human Teeth Biobank at the Federal University of Paraná (HTB-UFPR), the most sought-after services, whether externally obtained teeth are used and the degree of user satisfaction. For this, a semi-structured electronic questionnaire was applied to the internal community of the Dentistry course at UFPR, between August and December 2019. A total of 300 questionnaires were answered, 263 by undergraduate students, 4 by graduate students, 24 by professors and 9 by technical-administrative employees. Most students were female between 17 and 22 years old, while for employees(teachers and technicians) there was a similar distribution between the sexes in the age group between 40 and 59 years old. Although 80.99% of the students claimed to know the HTB-UFPR, only 50.19% used its services and 59.32% reported having already looked for teeth externally. The most used service was the withdrawalfor teaching activities, but only 42.15% returned them after use. The teeth obtained externally came mainly from private offices and only 56.33% of the students reported having always submitted them to disinfection/sterilization, with the autoclave being the most indicated method. All employees knew the HTB-UFPR and, among the teachers, most made both withdrawaland donations. The main purpose of the withdrawals was the research and 63.6% reported having returned their teeth. As for satisfaction, the academic community was predominantly fully satisfied. Finally, it emphasizes the need to expand awareness actions for the academic community regarding ethical, legal and biosafety aspects in the acquisition and manipulation of human teeth (AU).


Assuntos
Humanos , Masculino , Feminino , Adolescente , Adulto , Pessoa de Meia-Idade , Satisfação Pessoal , Dente/anatomia & histologia , Contenção de Riscos Biológicos/ética , Educação em Odontologia/métodos , Ética Odontológica/educação , Conhecimentos, Atitudes e Prática em Saúde , Inquéritos e Questionários/estatística & dados numéricos , Interpretação Estatística de Dados , Bancos de Espécimes Biológicos/ética
5.
Restor Dent Endod ; 46(2): e26, 2021 May.
Artigo em Inglês | MEDLINE | ID: mdl-34123762

RESUMO

OBJECTIVES: The aim of the present systematic review was to investigate the cryopreservation process of dental pulp mesenchymal stromal cells and whether cryopreservation is effective in promoting cell viability and recovery. MATERIALS AND METHODS: This systematic review was developed in accordance with the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) statement and the research question was determined using the population, exposure, comparison, and outcomes strategy. Electronic searches were conducted in the PubMed, Cochrane Library, Science Direct, LILACS, and SciELO databases and in the gray literature (dissertations and thesis databases and Google Scholar) for relevant articles published up to March 2019. Clinical trial studies performed with dental pulp of human permanent or primary teeth, containing concrete information regarding the cryopreservation stages, and with cryopreservation performed for a period of at least 1 week were included in this study. RESULTS: The search strategy resulted in the retrieval of 185 publications. After the application of the eligibility criteria, 21 articles were selected for a qualitative analysis. CONCLUSIONS: The cryopreservation process must be carried out in 6 stages: tooth disinfection, pulp extraction, cell isolation, cell proliferation, cryopreservation, and thawing. In addition, it can be inferred that the use of dimethyl sulfoxide, programmable freezing, and storage in liquid nitrogen are associated with a high rate of cell viability after thawing and a high rate of cell proliferation in both primary and permanent teeth.

6.
Dent Traumatol ; 37(4): 647-652, 2021 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-33547850

RESUMO

This report describes the unexpected formation of root-like structures following the avulsion of immature permanent teeth without replantation. A 6-year-old female patient had avulsed the four permanent mandibular incisors and the two deciduous mandibular canines. The patient was seen in an emergency healthcare unit but did not receive specialized treatment for tooth replantation. As follow-up treatment, she received a removable prosthesis. After 4 years of follow-up, an image obtained by panoramic radiography showed formations similar to four root structures in the alveolus of the previously avulsed permanent teeth. This finding was confirmed by periapical radiography and computed tomography. This case report demonstrates that in teeth with incomplete root development, even after avulsion without replantation, cells from the pulp stump may have the capacity to form mineralized structures that appear radiographically comparable to root dentin.


Assuntos
Avulsão Dentária , Criança , Necrose da Polpa Dentária , Dentição Permanente , Feminino , Humanos , Incisivo/diagnóstico por imagem , Avulsão Dentária/diagnóstico por imagem , Reimplante Dentário
7.
Clin Oral Investig ; 23(5): 2475-2488, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-30311063

RESUMO

OBJECTIVES: The purpose of this systematic review was to evaluate evidence about the prevalence of degenerative joint disease (DJD) of the temporomandibular joints (TMJ). MATERIALS AND METHODS: We performed search on electronic databases and gray literature from their inception to January 2018. Studies reporting prevalence data of DJD on TMJ were included. DJD was assessed through clinical and imaging diagnosis. Studies risk of bias was evaluated using the Critical Appraisal Checklist for Studies Reporting Prevalence Data. RESULTS: From 1082 studies, 32 were identified, and the sample size included 3435 subjects. They were clustered into two groups: the first comprised studies that reported prevalence of DJD in TMJ secondary to rheumatic systemic diseases like juvenile idiopathic arthritis (JIA) and rheumatoid arthritis (RA) and the second group comprised studies that reported prevalence of DJD on temporomandibular disorder patients. The prevalence of DJD on JIA patients ranged from 40.42% (n = 47) to 93.33% (n = 15) and on RA patients from 45.00% (n = 20) to 92.85% (n = 56). Among TMD patients, the prevalence of DJD reported according to patients ranged from 18.01% (n = 1038) to 84.74% (n = 118) and reported according to joints ranged from 17.97% (n = 178) to 77.23% (n = 224). CONCLUSION: This review attempts to high prevalence of DJD in patients with systemic rheumatic disease and a less prevalent, but still high, occurrence in patients with TMD without systemic involvement. CLINICAL RELEVANCE: Specialist doctors and dentists should be alert to not underestimate and to correctly diagnose DJD of the TMJ early in patients with rheumatic disease and TMD.


Assuntos
Osteoartrite/epidemiologia , Articulação Temporomandibular/patologia , Artrite Juvenil/complicações , Artrite Reumatoide/complicações , Humanos , Prevalência , Transtornos da Articulação Temporomandibular/complicações
8.
J Conserv Dent ; 21(2): 198-204, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-29674825

RESUMO

Aim: This study aims to evaluate the microshear bond strength of zirconia-based ceramics with different resin cement systems and surface treatments. Materials and Methods: Forty blocks of zirconia-based ceramic were prepared and embedded in polyvinyl chloride (PVC) tubes with acrylic resin. After polishing, the samples were washed in an ultrasonic bath and dried in an oven for 10 min. Half of the samples were subjected to sandblasting with aluminum oxide. Blocks were divided into four groups (n = 10) in which two resin cements were used as follows: (1) RelyX™ U100 with surface-polished zirconia; (2) RelyX™ U100 with surface-blasted zirconia; (3) Multilink with surface-polished zirconia; and 4) Multilink with surface-blasted zirconia. After performing these surface treatments, translucent tubes (n = 30 per group) were placed on the zirconia specimens, and resin cement was injected into them and light cured. The PVC tubes were adapted in a universal testing machine; a stiletto blade, which was bolted to the machine, was positioned on the cementation interface. The microshear test was performed at a speed of 0.5 mm/min. Failure mode was analyzed in an optical microscope and classified as adhesive, cohesive, or mixed. Results: The null hypothesis of this study was rejected because there was a difference found between the resin cement and the surface treatment. There was a statistical difference (P < 0.005) in RelyX™ U100 with surface-blasted zirconia, in relation to the other three groups. For Multilink groups, there was no statistical difference between them. Conclusion: Self-adhesive resin cement showed a more significant tendency toward bond strength in the ceramic-based zirconium oxide grit-blasted surfaces.

9.
Scanning ; 2017: 9650420, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-29109829

RESUMO

The aim of this study was to evaluate in fibroblast cultures the direct cytotoxic effects of etch-and-rinse, self-etch, and universal adhesive systems. The sterile glass cover slips (n = 3) were then immersed in culture medium to obtain the eluates for the experimental groups: (1) Adper™ Single Bond 2; (2) Ambar; (3) Adper™ Scotchbond™ Multi-Purpose; (4) Scotchbond™ Universal; (5) Ambar Universal; and (6) OptiBond All-In-One. As a negative control, sterile glass cover slips were immersed in culture medium only. After 24 h, the eluate obtained was applied on fibroblast culture. Cell viability and cell morphology were evaluated by MTT assay and SEM, respectively. Data were analyzed by Kruskal-Wallis and Mann-Whitney tests (α = 0.05). All adhesive systems except universal reduced cell viability in 3T3 cells to between 26.04% and 56.57%, and Scotchbond Universal and Ambar Universal reduced cell viability to 2.13% and 3.57%, respectively, when compared to the negative control. Cytoplasmic membrane shrinkage and cell-free areas with residual membrane fragments from dead cells were observed. In conclusion, improvements in universal adhesive system formulations and their mechanisms of action are not accompanied by increased toxicity compared with those in other systems, warranting commitment to the use of these dentin-pulp complexes.


Assuntos
Sobrevivência Celular/efeitos dos fármacos , Cimentos Dentários/toxicidade , Fibroblastos/efeitos dos fármacos , Fibroblastos/fisiologia , Células 3T3 , Animais , Membrana Celular/patologia , Forma Celular/efeitos dos fármacos , Fibroblastos/citologia , Formazans/análise , Camundongos , Microscopia Eletrônica de Varredura , Sais de Tetrazólio/análise
10.
Dent. press endod ; 7(3): 34-42, set.-dec. 2017. ilus
Artigo em Português | LILACS, BBO - Odontologia | ID: biblio-877447

RESUMO

Introdução: a reabsorção radicular é um processo patológico que resulta na perda de cemento, dentina e osso, sendo desencadeada por desequilíbrio entre osteoblastos e osteoclastos. O diagnóstico geralmente ocorre por meio de exame radiográfico e/ou tomográfico. Objetivo: relatar o tratamento de um caso de reabsorção cervical no dente #14 com abordagem multidisciplinar envolvendo Endodontia, Ortodontia, Periodontia e Prótese. Métodos: após realização de tomografia, verificou-se que a imagem correspondia a uma reabsorção cervical externa na raiz palatina, estendendo-se desde distal, seguindo para palatal e mesial. Após abertura e medicação intracanal, o endodontista optou por encaminhar a paciente para avaliação com ortodontista, sendo sugerida a realização de tracionamento ortodôntico, numa tentativa de expor a área da lesão. Para se atingir a posição final do dente, entre ativações de segunda e terceira ordens e ajustes oclusais, a etapa ortodôntica durou onze meses, incluindo três meses de contenção. Um mês antes de remover o aparelho ortodôntico e após um ano desde o início do tracionamento, realizou-se a obturação dos canais radiculares. Ainda com o dispositivo ortodôntico, foi realizada a cirurgia para correção do nível ósseo e gengival, que tende a acompanhar o dente, visualizando-se totalmente a área da reabsorção. Após um mês da cirurgia de aumento de coroa clínica, removeu-se o aparelho ortodôntico e realizou-se o tratamento protético com núcleo metálico fundido com liga de ouro e coroa em cerâmica com infraestrutura de zircônia. Conclusão: a partir de uma abordagem multidisciplinar, torna-se possível o tratamento de casos de reabsorções cervicais externas.


Assuntos
Humanos , Feminino , Adulto , Tomografia Computadorizada de Feixe Cônico , Prótese Dentária , Endodontia , Extrusão Ortodôntica , Reabsorção da Raiz/terapia , Reabsorção de Dente/terapia , Osteoclastos
11.
Indian J Dent Res ; 28(1): 59-65, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-28393819

RESUMO

BACKGROUND: The present study was conducted to evaluate the bond strength in the different root thirds (premolars and maxillary central incisors) of composite relined glass fiber posts compared to untreated glass fiber posts cemented with dual- or chemical-cure cements. MATERIALS AND METHODS: Sixty human single-rooted premolars (flat canal) (n = 15) and 12 maxillary central incisors were used (round canal) (n = 3). The teeth were sectioned, and the roots received endodontic treatment. The standardized preparation of the canals was carried out, and the roots were randomly divided into four groups according to the cementation systems: G1: cemented posts (dual: Ambar/Allcem); G2: relined posts (dual: Ambar/Allcem); G3: cemented posts (chemical: Fusion Duralink/Cement Post); and G4: relined posts (chemical: Fusion Duralink/Cement Post). The roots were cut to give two slices of each third of the root canal per specimen. Push-out test was conducted at a speed of 0.5 mm/min. Data were analyzed by analysis of variance and Tukey's post hoc test (α = 0.05). RESULTS: There was no statistically significant difference between groups for the premolars (flat canal) (P = 0.959). There was a significant difference in the central incisors between the middle and apical thirds in the cemented group when using the dual system (P = 0.04) and between the middle and apical thirds (P = 0.003) and cervical and apical thirds (P = 0.033) when using the chemical system. CONCLUSION: Due to the anatomy of the root canal, flat canal of the premolars does not require relining, but round canal of the maxillary central incisors demands it for more secure in the bond strength.


Assuntos
Resinas Acrílicas , Cimentação/métodos , Resinas Compostas , Cavidade Pulpar , Vidro , Poliuretanos , Técnica para Retentor Intrarradicular , Óxido de Alumínio , Dente Pré-Molar , Porcelana Dentária , Humanos , Técnicas In Vitro , Incisivo , Metacrilatos , Propriedades de Superfície
12.
Artigo em Inglês | MEDLINE | ID: mdl-27765328

RESUMO

OBJECTIVE: To evaluate the diagnostic validity of clinical examination protocols compared with magnetic resonance imaging (MRI) in adults with temporomandibular joint disk displacement disorders. STUDY DESIGN: According to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines, a systematic review was undertaken using a selection process in 2 phases; 283 different references were identified, and 10 articles were included for qualitative analysis and 7 for meta-analysis. Temporomandibular joint disorders were assessed through clinical diagnosis protocols with the aid of Research Diagnostic Criteria for Temporomandibular Disorders, Diagnostic Criteria for Temporomandibular Disorders, or Clinical Diagnostic Criteria for Temporomandibular Disorders. The authors searched the following electronic databases: Cochrane, LILACS, PubMed, Science Direct, SCOPUS, and Web of Science. Additional search of gray literature was performed. Selected studies were evaluated by using the Quality Assessment Tool for Diagnostic Accuracy Studies-2. Two subgroups were analyzed: Disk displacement with reduction (DDwR) and disk displacement without reduction (DDwoR). RESULTS: The validity of clinical protocols compared with MRI performed in studies evaluating only DDwR presented sensitivity of 44% (39%-49%) and specificity 51% (46%-57%). In studies evaluating only DDwoR, sensitivity was 22% (16%-30%) and specificity 93% (85%-98%). The area under the curve value for validity of clinical protocols in all studies was 0.63, 0.56 for studies evaluating DDwR and 0.64 for studies evaluating DDwoR. Area under the curve values were considered poor. CONCLUSIONS: Clinical examination protocols have poor validity to diagnose DDwR and DDwoR compared with MRI. MRI should be used to increase the diagnostic accuracy when the information provided can influence clinical decisions.


Assuntos
Protocolos Clínicos , Imageamento por Ressonância Magnética , Transtornos da Articulação Temporomandibular/diagnóstico , Humanos , Sensibilidade e Especificidade , Revisões Sistemáticas como Assunto
14.
J Anal Methods Chem ; 2015: 286812, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-26075139

RESUMO

A simple stability-indicating HPLC-DAD method was validated for the determination of ferulic acid (FA) in polymeric microparticles. Chromatographic conditions consisted of a RP C18 column (250 mm × 4.60 mm, 5 µm, 110 Å) using a mixture of methanol and water pH 3.0 (48 : 52 v/v) as mobile phase at a flow rate of 1.0 mL/min with UV detection at 320 nm. The developed method was validated as per ICH guidelines with respect to specificity, linearity, limit of quantification, limit of detection, accuracy, precision, and robustness provided suitable results regarding all parameters investigated. The calibration curve was linear in the concentration range of 10.0-70.0 µg/mL with a correlation coefficient >0.999. Precision (intraday and interday) was demonstrated by a relative standard deviation lower than 2.0%. Accuracy was assessed by the recovery test of FA from polymeric microparticles (99.02% to 100.73%). Specificity showed no interference from the components of polymeric microparticles or from the degradation products derived from acidic, basic, and photolytic conditions. In conclusion, the method is suitable to be applied to assay FA as bulk drug and into polymeric microparticles and can be used for studying its stability and degradation kinetics.

15.
Arch Oral Biol ; 60(8): 1138-45, 2015 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-26042623

RESUMO

OBJECTIVE: This study investigated the effect of a self-etching adhesive system containing an antimicrobial quaternary ammonium methacrylate polymer (QAMP) on the vascular permeability using Evans blue and laser-Doppler flowmetric methods. DESIGN: Forty rats were anesthetized and divided into groups: saline solution; Clearfil™ Protect Bond; Clearfil™ SE Bond; Clearfil™ SE Bond containing QAMP. Injections of Evans blue were administrated intravenously and the substances were injected intradermally. Immediately before injection and after 3 and 6h, was evaluated the blood flow. The animals were killed 3 and 6h after injection. The dorsal skin was dissected and experimental sites were obtained. The vascular permeability was evaluated by dye extravasation area, the dye was extracted and absorbance measured. RESULTS: Concerning the Evans blue method, Clearfil™ SE Bond containing QAMP showed an extravasation area statistically similar to Clearfil™ SE Bond and lower than Clearfil™ Protect Bond. No statistical difference was verified among experimental groups for the amount of dye extracted. Clearfil™ SE Bond containing QAMP provided better laser-Doppler flowmetric parameters than Clearfil™ Protect Bond immediately and 3h after injection. CONCLUSION: The QAMP had lower effect on the exudative phase of the inflammatory process.


Assuntos
Permeabilidade Capilar/efeitos dos fármacos , Adesivos Dentinários/química , Compostos de Amônio Quaternário/química , Cimentos de Resina/química , Animais , Azul Evans , Extravasamento de Materiais Terapêuticos e Diagnósticos , Fluxometria por Laser-Doppler , Teste de Materiais , Testes de Sensibilidade Microbiana , Ratos , Tela Subcutânea , Propriedades de Superfície
16.
Rev. odontol. UNESP (Online) ; 44(2): 80-84, Mar-Apr/2015. tab
Artigo em Português | LILACS, BBO - Odontologia | ID: lil-746234

RESUMO

Objetivo: Avaliar a liberação e a capacidade de recarga de flúor de diferentes cimentos de ionômero de vidro, durante a simulação de desafio cariogênico. Material e método: Foram confeccionados 12 corpos de prova para cada grupo experimental, com cimentos de ionômero de vidro convencionais - Maxxion R (FGM), Ketac Molar EasyMix (3M ESPE); cimento de ionômero de vidro modificado por resina - Vitrebond (3M ESPE), e resina composta, Filtek™ Z350XT (3M ESPE), como controle negativo. Os corpos de prova foram imersos alternadamente em sistema de ciclagem de pH, permanecendo 6 horas na solução de desmineralização e 18 horas na de remineralização, sendo mantidos em estufa a 37°C. Liberação de flúor foi verificada 1, 2, 7 e 14 dias antes e após a recarga com flúor, com eletrodo específico acoplado ao aparelho analisador de pH/fluoretos, calibrado a cada medição com soluções de fluoreto de sódio a 1 e 10 ppm, preparadas com TISAB II. Para leitura, foi adicionado 0,5 mL da amostra a igual volume de TISAB II. Recarga foi realizada com flúor neutro 2% por 4 minutos nas amostras, lavadas e imersas novamente nas soluções do sistema de ciclagem de pH. Os dados foram submetidos à análise de variância e ao teste de Tukey (α=0,05). Resultado: A liberação inicial de fluoreto (µgF/cm2) foi de 45,36; 37,49, e 26,35 para Maxxion R, Vitrebond e Ketac Molar EasyMix, respectivamente. Diferenças estatísticas significativas entre os materiais foram verificadas antes e após a recarga (p=0,001). Após a aplicação tópica de flúor, os cimentos de ionômero de vidro apresentaram capacidade de recarga de flúor. Conclusão: Cimentos de ionômero de vidro avaliados foram capazes de liberar flúor em soluções de ciclagem de pH e podem recarregar flúor através da aplicação tópica.


Objective: To evaluated the ability to fluoride release and recharge in the different glass ionomer cements during simulation of cariogenic challenge. Material and method: Twelve samples were made for each experimental group: conventional glass ionomer cements, Maxxion R (FGM), Ketac Molar EasyMix (3M ESPE); resin modified glass ionomer cement, Vitrebond (3M ESPE); and composite resin n Filtek™Z350XT (3M ESPE), negative control. The samples were immersed alternately in pH cycling method, remaining 6 hours in demineralization solution and 18 hours in remineralization solution and maintained at 37 °C. Fluoride release was measured 1, 2, 7, and 14 days before and after recharging with specific electrode coupled to the analyzer apparatus pH/fluoride, each measurement with calibrated solutions of sodium fluoride to 1 and 10 ppm, prepared with TISAB II. For reading was added 0.5 mL of sample to an equal volume of TISAB II. Recharging was made with neutral sodium fluoride to 2% for 4 minutes in the samples, washed and again immersed pH cycling solutions. Data were subjected to two-way ANOVA and Tukey's post-hoc test (α=0.05). Result: The initial fluoride release (µgF/cm2) was 45.36 ; 37.49 and 26.35 for Maxxion R, Vitrebond and Ketac Molar EasyMix, respectively. Significant differences between the materials before and after recharging were observed (p=0.001). After topical application of fluoride, both showed ability to recharge. Conclusion: The glass ionomer cements evaluated were capable of releasing fluoride in pH cycling solutions and may recharge through of a topical application.


Assuntos
Fluoreto de Sódio , Desmineralização , Análise de Variância , Resinas Compostas , Flúor , Cimentos de Ionômeros de Vidro , Concentração de Íons de Hidrogênio
17.
RGO (Porto Alegre) ; 61(supl.1)jul.-dez. 2013.
Artigo em Português | LILACS-Express | LILACS | ID: lil-702846

RESUMO

O reconhecimento da importância do elemento dental como órgão humano é um fato muitas vezes negligenciado ou desconsiderado pela maioria dos odontólogos e por alguns profissionais vinculados à pesquisa científica, que utilizam grandes quantidades de dentes humanos em seus trabalhos, e por acadêmicos dos cursos de Odontologia. O presente estudo tem como propósito rever os aspectos éticos e legais envolvidos na utilização de dentes humanos por todos os envolvidos, sejam eles pesquisadores, professores e/ou alunos, promovendo assim um uso racional desses órgãos, o que vem sendo possível com a criação de Bancos de Dentes nas universidades. Diante do exposto, torna-se necessária a existência de um vínculo entre o Banco de Dentes e o Comitê de Ética da Instituição em que este se encontra, a fim de que se estabeleçam, desde o início de sua organização, diretrizes e normas de funcionamento conjuntas, visando ao cumprimento da legislação vigente.


The perception of the dental element as a human organ is neglected and disregarded many times by most of the dentists that use a great amount of human teeth in their works, by some of the professionals linked to scientific research, by professors and also by academics of dentistry courses. The main goal of this study is to review in the literature, the ethical and legal aspects involved in the use of human teeth in universities, demonstrating that that perception should be implicit in all its users, whether researchers, professors and/or students, resulting in a rational use of those organs, such as is possible with the creation of Bank of Teeth in universities. Facing the foregoing, it is necessary the existence of a link between the Bank of Teeth and the Ethics Institutional Committee of the university, in order to be establish guidelines, rules, standards and mode operations since the beginning of the bank organization, aimed at compliance with current legislation.

18.
J Biomater Sci Polym Ed ; 24(12): 1443-58, 2013.
Artigo em Inglês | MEDLINE | ID: mdl-23829457

RESUMO

A quaternary ammonium methacrylate polymer (QAMP) with antimicrobial potential was synthesized. The resulting product (QAMP) was characterized by FTIR spectroscopy, NMR spectroscopy, visible spectrophotometry, XRPD and TGA. The in vitro susceptibility tests against Streptococcus mutans of QAMP were investigated prior and after incorporation into a commercial adhesive system (Clearfil™ SE Bond). The release of quaternary ammonium compounds from the experimental adhesive system (Clearfil™ SE Bond + 5% QAMP) was performed during 1, 7, 14, 21 and 30 days. Spectroscopic data confirmed that QAMP was successfully obtained. Thermogravimetric analysis indicated that QAMP was heat stable. Prior incorporation into the adhesive system, QAMP revealed an inhibition halo of 18.33 ± 0.6 mm. By agar disk diffusion test, Clearfil™ SE Bond containing 5% QAMP presented an inhibition halo (16.67 ± 1.5 mm) similar to Clearfil™ Protect Bond (positive control, 17.00 ± 1.7, p = 0.815) and significantly higher than Clearfil™ SE Bond (negative control, 11.00 ± 1.0, p = 0.006). The minimum inhibitory/bactericidal concentrations for Clearfil™ SE Bond containing 5% QAMP were 20 µL mL(-1). The release of quaternary ammonium compounds from the experimental adhesive containing QAMP was very low (5.1%) when compared to Clearfil™ Protect Bond that released 47.2% of its quaternary ammonium monomer (MDPB) after 30 days. The QAMP can offer enhanced antimicrobial properties for self-etching adhesive systems.


Assuntos
Anti-Infecciosos Locais/química , Anti-Infecciosos Locais/farmacologia , Cimentos Dentários/química , Metacrilatos/química , Compostos de Amônio Quaternário/química , Cimentos Dentários/farmacologia , Metacrilatos/farmacologia , Polímeros , Compostos de Amônio Quaternário/farmacologia , Espectroscopia de Infravermelho com Transformada de Fourier , Streptococcus/efeitos dos fármacos , Streptococcus/metabolismo
19.
J Conserv Dent ; 15(2): 132-6, 2012 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-22557810

RESUMO

AIM: This study assessed the effect of the dentin depth and the application mode on the hardness and elastic modulus of the adhesive layer. MATERIALS AND METHODS: Occlusal surfaces of 48 caries-free human third molars were removed, at two levels: Superficial and deep dentin. For each type of surface, the test specimens were randomly divided into groups which underwent the application: A conventional two-step adhesive system (Adper™ Single Bond [SB]) and self-etch adhesives system (Adper™ SE Plus [SE] and AdheSE(®) [AD]). The adhesives applied were active or passive. Composite build-ups were constructed incrementally. The teeth were sectioned, embedded, and polished. The nanoindentation test was performed in the adhesive layer. The results were analyzed by ANOVA and Tukey tests. RESULTS: In the adhesive layer, the higher hardness (0.307 ± 0.006 GPa) and elastic modulus (4.796 ± 0.165 GPa) of SE were obtained in superficial dentin in passive application. The elastic modulus of SE (4.115 ± 0.098 GPa) was lowest in active application in superficial dentin. The active application significantly increased the hardness of the SB in the deep dentin (0.011 ± 0.314 GPa) compared the superficial dentin (0.280 ± 0.010 GPa). For the AD, only the mode of application was statistically significant (P=0.0041) for the hardness, active application (0.289 ± 0.015 GPa) being higher than passive application (0.261 ± 0.013 GPa) (P=0.0042) in deep dentin. CONCLUSION: The experimental results reveal that the mechanical properties were influenced for the application mode of adhesive systems and dentin depth.

20.
Rev. Assoc. Paul. Cir. Dent ; 66(1): 48-52, jan.-mar. 2012.
Artigo em Português | LILACS, BBO - Odontologia | ID: lil-654821

RESUMO

Objetivo: avaliar se o polimento pode minimizar ou resolver o manchamento ocasionado porcorantes de diferentes bebidas em resina composta. Material e Métodos: Confeccionou-se 40corpos-de-prova (CP)de resina composta microhíbrida Opallis (FGM), cor B1,em matriz plástica (5mm de diâmetro e 2 mm de espessura). A fotoativação foi realizada por 30s com LEDmetron I.Aseguir os CP's foram submetidos à avaliação inicial de cor em espectrofotômetro VITA EasyshadeCompact" obtendo-se valores correspondentes da escala CIEL*a*b*. Os CP foram divididos em 4grupos (n=10): água destilada (controle), café, Coca-Colaw e vinho tinto. Realizou-se avaliaçãoinicial da cor, após imersão por 60 dias para promover o manchamento e, após o procedimentode polimento com discos de feltro e pasta diamantada. Os dados foram analisados pela ANOVA2 fatores e pós-teste de Tukey (o=O,OS).Resultados: As médias ± desvio padrão de L* mostraramque a resina composta apresentou manchamento (p>O,OOl) após 60 dias de imersão em vinho(S7,96±7,98) e no café (61,89±3,S9) quando comparado a água destilada (73,49± 1,20) e a Coca--Cola" (70,SO±1,30). O polimento não conseguiu melhoria no manchamento (p

Objective: To assesswhether the polishing can minimize or solve the staining caused by dyes ofdifferent beverages in composite resins. Methods: 40 specimens were fabricated with composite resinmicrohybrid Opallis (FGM), B1 color in the plastic matrix (S mm in diameter and 2 mm thickl Thepolymerization was carried out with 30 seconds LEDmetron I.Then the specimens underwent initialevaluation of color spectrophotometer VITA Easyshade ® Compact obtaining corresponding valuesof the scale CIEL*a*b", The specimens were divided into four groups (n = 10): distilled water (controllcoffee, Coca-Colae and red wine. The specimens were again subjected to color evaluation initial,after the immersion for 60 days to promote the staining, and after the polishing procedure (felt discwith diamond paste). Data were analyzed by two-away ANOVA and Tukev's HSD test (0= O.OS).Results:mean ± standard deviation of L* showed that the composite showed staining (p¼ 0.001) after60 days of immersion in wine (S7.96 ± 7.98) and coffee (61.89 ± 3.S9) when compared to distilledwater (73.49 ± 1.20) and Coca-Cola ® (70.S0 ± 1.30).The polishing could not improve the staining (p<0.001) caused by wine and coffee. Conclusion: the polishing didn't minimize the stains caused bywine and coffee and such changes considered clinically detectable to the human eye.


Assuntos
Humanos , Masculino , Feminino , Polimento Dentário , Espectrofotometria/métodos , Resinas Compostas/uso terapêutico
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