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1.
IEEE Trans Cybern ; 2019 Dec 25.
Artigo em Inglês | MEDLINE | ID: mdl-31880574

RESUMO

We propose a fast online video pose estimation method to detect and track human upper-body poses based on a conditional dynamic Bayesian modeling of pose modes without referring to future frames. The estimation of human body poses from videos is an important task with many applications. Our method extends fast image-based pose estimation to live video streams by leveraging the temporal correlation of articulated poses between frames. Video pose estimation is inferred over a time window using a conditional dynamic Bayesian network (CDBN), which we term time-windowed CDBN. Specifically, latent pose modes and their transitions are modeled and co-determined from the combination of three modules: 1) inference based on current observations; 2) the modeling of mode-to-mode transitions as a probabilistic prior; and 3) the modeling of state-to-mode transitions using a multimode softmax regression. Given the predicted pose modes, the body poses in terms of arm joint locations can then be determined more accurately and robustly. Our method is suitable to investigate high frame rate (HFR) scenarios, where pose mode transitions can effectively capture action-related temporal information to boost performance. We evaluate our method on a newly collected HFR-Pose dataset and four major video pose datasets (VideoPose2, TUM Kitchen, FLIC, and Penn_Action). Our method achieves improvements in both accuracy and efficiency over existing online video pose estimation methods.

2.
Leukemia ; 2019 Nov 27.
Artigo em Inglês | MEDLINE | ID: mdl-31776466

RESUMO

Recurrent oncogenic mutations of MyD88 have been identified in a variety of lymphoid malignancies. Gain-of-function mutations of MyD88 constitutively activate downstream NF-κB signaling pathways, resulting in increased cellular proliferation and survival. However, whether MyD88 activity can be aberrantly regulated in MyD88-wild-type lymphoid malignancies remains poorly understood. SPOP is an adaptor protein of CUL3-based E3 ubiquitin ligase complex and frequently mutated genes in prostate and endometrial cancers. In this study, we reveal that SPOP binds to and induces the nondegradative ubiquitination of MyD88 by recognizing an atypical SPOP-binding motif in MyD88. This modification blocks Myddosome assembly and downstream NF-κB activation. SPOP is mutated in a subset of lymphoid malignancies, including diffuse large B-cell lymphoma (DLBCL). Lymphoid malignancies-associated SPOP mutants exhibited impaired binding to MyD88 and suppression of NF-κB activation. The DLBCL-associated, SPOP-binding defective mutants of MyD88 escaped from SPOP-mediated ubiquitination, and their effect on NF-κB activation is stronger than that of wild-type MyD88. Moreover, SPOP suppresses DLBCL cell growth in vitro and tumor xenograft in vivo by inhibiting the MyD88/NF-κB signaling. Therefore, SPOP acts as a tumor suppressor in DLBCL. Mutations in the SPOP-MyD88 binding interface may disrupt the SPOP-MyD88 regulatory axis and promote aberrant MyD88/NF-κB activation and cell growth in DLCBL.

3.
World J Clin Cases ; 6(15): 931-935, 2018 Dec 06.
Artigo em Inglês | MEDLINE | ID: mdl-30568948

RESUMO

AIM: To compare the outcomes of retrograde intrarenal surgery (RIRS) and miniaturized percutaneous nephrolithotomy (mini-PCNL) in treating lower pole (LP) renal stones with a diameter of 1.5-2.5 cm. METHODS: A total of 216 patients who underwent mini-PCNL (n = 103) or RIRS n = 113) for LP stones with a diameter of 1.5-2.5 cm were enrolled between December 2015 and April 2017 at the Urology Department of Ningbo Urology and Nephrology Hospital. RESULTS: Significant differences were found in the hospital stay (9.39 ± 4.01 vs 14.08 ± 5.26, P < 0.0001) and hospitalization costs (2624.5 ± 513.36 vs 3255.2 ± 976.5, P < 0.0001) between the RIRS and mini-PCNL groups. The mean operation time was not significantly different between the RIRS group (56.48 ± 24.77) and the mini-PCNL group (60.04 ± 30.38, P = 0.345). The stone-free rates at the first postoperative day (RIRS vs mini-PCNL: 90.2% vs 93.2%, P = 0.822) and the second month postoperatively (RIRS vs mini-PCNL: 93.8% vs 95.1%, P = 0.986) were not significantly different. CONCLUSION: RIRS and mini-PCNL are both safe and effective methods for treating LP stones with a diameter of 1.5-2.5 cm. RIRS can be considered as an alternative to PCNL for the treatment for LP stones of 1.5-2.5 cm.

4.
J Cancer Res Ther ; 14(Supplement): S713-S718, 2018 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-30249892

RESUMO

Aims: Tat-interacting protein 30 (TIP30), a transcriptional repressor, possesses antitumor effect in different cancer cells. However, little is known about the function of TIP30 in bladder cancer till now. Materials and Methods: A TIP30-overexpressing plasmid was transfected into the bladder cancer cells (T24). The cell cycle and apoptosis were detected by flow cytometry. The cell proliferation was analyzed using the cell counting kit-8 assay. The migrative and invasive abilities of T24 cells were measured by the transwell assay. The expression of TIP30, cell cycle proteins, migration-related proteins, and cell apoptosis-related proteins was assessed by Western blotting. Results: The cell proliferation, migration, and invasion of T24 cells were inhibited by overexpression of TIP30. Moreover, the rate of cell apoptosis was increased by the overexpression of TIP30. The expression of cell cycle proteins, phosphorylated EGFR, p-Akt, Bcl-2, cyclin D, cyclin E), migration-related proteins (matrix metalloproteinases 2 [MMP2], MMP6, MMP9), were downregulated, and cell apoptosis-related proteins (bax, cleaved caspase3) were upregulated. Conclusions: These results suggest that TIP30, as a tumor suppressor in the bladder cancer, might be served as a target in cancer therapies in the future.


Assuntos
Acetiltransferases/genética , Proliferação de Células/genética , Proteínas de Neoplasias/genética , Fatores de Transcrição/genética , Neoplasias da Bexiga Urinária/genética , Apoptose/genética , Ciclo Celular/genética , Linhagem Celular Tumoral , Movimento Celular/genética , Citometria de Fluxo , Regulação Neoplásica da Expressão Gênica/genética , Humanos , Invasividade Neoplásica/genética , Invasividade Neoplásica/patologia , Neoplasias da Bexiga Urinária/patologia
5.
Onco Targets Ther ; 11: 3267-3280, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-29910622

RESUMO

Background: The vitamin D receptor (VDR) plays a key role in vitamin-mediated signaling pathway. Emerging evidence has suggested that the VDR polymorphism may contribute to the risk of prostate cancer (PCa). However, the existing results are not conclusive in Asian population. Methods: We aim to evaluate the potential role of VDR polymorphisms on PCa of Asian population. PubMed, Scopus, Embase, Web of Science, Chinese National Knowledge Infrastructure, Wang Fang Data, and VIP Periodical were retrieved, and eligible studies (case-control or cohort study) meeting the inclusion criteria were evaluated through an updated meta-analysis using Stata13.0 software. Results: A total of 1,363 cases and 2,101 controls obtained from 13 eligible publications were eventually included in this meta-analysis. Our results show that a significant association of VDR taq1 polymorphism with PCa risk, especially in the Japanese population. In the clinical stage-stratified analysis, the pooled results revealed no significant difference in genetic polymorphisms between the local stage and control groups, whereas there was increased frequency of T allele and TT genotype in the advanced tumor stage group compared with local tumor stage or control groups. Similarly, no significant difference was seen in Gleason <7 and control groups, but the T allele and TT genotype were significantly higher in the Gleason ≥7 group compared with Gleason <7 or control groups. Conclusion: The VDR TaqI polymorphism might be associated with PCa risk in Asian population, especially in the Japanese population. Also, PCa patients carrying the T allele or TT genotype were more likely to progress to advanced stage. These results suggest that VDR TaqI polymorphisms may be potential diagnostic biomarkers for PCa susceptibility.

6.
Biomed Pharmacother ; 102: 326-332, 2018 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-29571017

RESUMO

Increasing evidence has indicated the important roles of long non-coding RNAs (lncRNAs) in tumorigenesis and cellular progression, including prostate cancer. In this study, we aim to investigate the expression level of SNHG7 and its biological functions on prostate cancer cells. Results indicated that SNHG7 expression was significantly up-regulated in prostate cancer tissue and cell lines. Besides, the overexpression of SNHG7 was closely correlated with the poor prognosis. In vitro and in vivo, experiments demonstrated that SNHG7 knockdown markedly inhibited prostate cancer proliferation and cycle-related protein (CDK4, CDK6, Cyclin D1), induced cell cycle arrest at G0/G1 phase and suppressed tumor growth. Moreover, miR-503 was predicted by bioinformatics tools and validated using luciferase reporter assay to both directly inhibited SNHG7 and Cyclin D1 expression by targeting their RNA 3'-UTR. In conclusion, results present that SNHG7 regulates the cycle progression and acts as an oncogenic gene in the prostate cancer tumorigenesis via miR-503/Cyclin D1 pathway, revealing the vital role of lncRNA/miRNA/mRNA axis in prostate cancer carcinogenesis.


Assuntos
Ciclo Celular/genética , Proliferação de Células/genética , Ciclina D1/metabolismo , MicroRNAs/metabolismo , Neoplasias da Próstata/genética , RNA Longo não Codificante/genética , Idoso , Animais , Linhagem Celular Tumoral , Técnicas de Silenciamento de Genes , Humanos , Masculino , Camundongos Nus , Pessoa de Meia-Idade , Neoplasias da Próstata/patologia , Regulação para Cima , Ensaios Antitumorais Modelo de Xenoenxerto
7.
Mol Med Rep ; 17(4): 6038-6044, 2018 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-29436671

RESUMO

This study explored the potential value of curcumin, a natural product, in the protection of CsA­induced nephrotoxicity. The aim of the present study was to investigate the effects of curcumin on Cyclosporine A (CsA)­induced renal oxidative stress and determine the potential underlying molecular mechanisms of the renal protective effects of Cur. HK­2 human renal cells were co­treated with CsA and various doses of Cur. Cell survival rate was determined by an MTT assay, total cellular protein was collected and oxidative stress in cell homogenates was evaluated by determining the activity of superoxide dismutase (SOD), glutathione peroxidase (GSH­Px) and catalase (CAT), the levels of malondialdehyde (MDA) and reactive oxygen species (ROS), and total antioxidant capacity. Furthermore, Bcl­2 and Bcl­2­associated X (Bax) protein expression was measured by western blot analysis. In addition, a CsA­induced nephrotoxicity (CAN) rat model was also established. Renal function was analyzed by measuring creatinine (Crea) and blood urea nitrogen (BUN) in the serum of rats, and histopathological examination was performed on renal tissues using hematoxylin and eosin staining, periodic acid­Schiff staining and nuclear factor­κB (NF­κB) immunostaining. The results demonstrated that treatment of HK­2 cells with CsA significantly increased ROS and MDA levels, and decreased the activities of SOD, GSH­Px and CAT, compared with the control group. However, these effects of CsA were dose­dependently improved by treatment with Cur. In addition, Cur treatment increased Bcl­2 and decreased Bax protein in HK­2 cells, compared with cells treated with CsA alone. In the CAN rat model CsA (30 mg/kg) treatment significantly elevated serum Crea levels and BUN, but lowered endogenous Crea clearance rate, compared with the control group. Co­administration of Cur with CsA significantly reversed the effects of CsA on serum Crea levels, BUN and Crea clearance rate (Ccr). Additionally, Cur alleviated CsA­induced renal cell injury, as less vacuolar degeneration of glomerular cells was observed compared with the CsA alone group. In conclusion, Cur may increase renal antioxidant capacity and reduce the Bax/Bcl­2 ratio, subsequently improving CsA­induced renal failure and renal tubular deformation and cell vacuolization.


Assuntos
Curcumina/farmacologia , Ciclosporina/efeitos adversos , Nefropatias/etiologia , Nefropatias/metabolismo , Rim/efeitos dos fármacos , Rim/metabolismo , Substâncias Protetoras/farmacologia , Animais , Apoptose/efeitos dos fármacos , Linhagem Celular , Modelos Animais de Doenças , Humanos , Rim/patologia , Nefropatias/tratamento farmacológico , Nefropatias/patologia , Testes de Função Renal , Masculino , Estresse Oxidativo/efeitos dos fármacos , Proteínas Proto-Oncogênicas c-bcl-2/genética , Proteínas Proto-Oncogênicas c-bcl-2/metabolismo , Ratos , Espécies Reativas de Oxigênio/metabolismo , Proteína X Associada a bcl-2/genética , Proteína X Associada a bcl-2/metabolismo
8.
IEEE Trans Image Process ; 27(4): 1809-1821, 2018 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-29346096

RESUMO

To effectively solve the challenges in object tracking, such as large deformation and severe occlusion, many existing methods use graph-based models to capture target part relations, and adopt a sequential scheme of target part selection, part matching, and state estimation. However, such methods have two major drawbacks: 1) inaccurate part selection leads to performance deterioration of part matching and state estimation and 2) there are insufficient effective global constraints for local part selection and matching. In this paper, we propose a new object tracking method based on iterative graph seeking, which integrate target part selection, part matching, and state estimation using a unified energy minimization framework. Our method also incorporates structural information in local parts variations using the global constraint. We devise an alternative iteration scheme to minimize the energy function for searching the most plausible target geometric graph. Experimental results on several challenging benchmarks (i.e., VOT2015, OTB2013, and OTB2015) demonstrate improved performance and robustness in comparison with existing algorithms.

9.
Oncol Lett ; 13(4): 2577-2582, 2017 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-28454436

RESUMO

Special AT-rich sequence-binding protein-1 (SATB1) is associated with cancer progression and poor clinical outcome. The present study aims to evaluate whether SATB1 affects the biological behaviors of prostate cancer (PCa), and furthermore, to elucidate whether this effect works through the epithelial-mesenchymal transition (EMT) pathway. Firstly, the expression of SATB1 was investigated in a series of PCa tissues as well as in a panel of PCa cell lines. Cell proliferation, migration and invasion were evaluated in SATB1 knockdown and overexpressed PCa cell lines by MTT and Transwell assays. The results showed that the expression of SATB1 was markedly upregulated in PCa tissues and all PCa cell lines (P<0.001). Ectopic expression of SATB1 promoted PCa cell proliferation and migration. Knockdown of SATB1 repressed the ability of cell proliferation and migration of PCa cells. In addition, inhibition of SATB1 could reverse the EMT processes through upregulation of E-cadherin and downregulation of vimentin. The present study provided evidence that SATB1 may act as a potential therapeutic target in PCa patients.

10.
IEEE Trans Cybern ; 47(12): 4182-4195, 2017 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-27875238

RESUMO

Graph-based representation is widely used in visual tracking field by finding correct correspondences between target parts in different frames. However, most graph-based trackers consider pairwise geometric relations between local parts. They do not make full use of the target's intrinsic structure, thereby making the representation easily disturbed by errors in pairwise affinities when large deformation or occlusion occurs. In this paper, we propose a geometric hypergraph learning-based tracking method, which fully exploits high-order geometric relations among multiple correspondences of parts in different frames. Then visual tracking is formulated as the mode-seeking problem on the hypergraph in which vertices represent correspondence hypotheses and hyperedges describe high-order geometric relations among correspondences. Besides, a confidence-aware sampling method is developed to select representative vertices and hyperedges to construct the geometric hypergraph for more robustness and scalability. The experiments are carried out on three challenging datasets (VOT2014, OTB100, and Deform-SOT) to demonstrate that our method performs favorably against other existing trackers.

11.
IEEE Trans Image Process ; 25(8): 3572-84, 2016 08.
Artigo em Inglês | MEDLINE | ID: mdl-27214901

RESUMO

Recent advances in online visual tracking focus on designing part-based model to handle the deformation and occlusion challenges. However, previous methods usually consider only the pairwise structural dependences of target parts in two consecutive frames rather than the higher order constraints in multiple frames, making them less effective in handling large deformation and occlusion challenges. This paper describes a new and efficient method for online deformable object tracking. Different from most existing methods, this paper exploits higher order structural dependences of different parts of the tracking target in multiple consecutive frames. We construct a structure-aware hyper-graph to capture such higher order dependences, and solve the tracking problem by searching dense subgraphs on it. Furthermore, we also describe a new evaluating data set for online deformable object tracking (the Deform-SOT data set), which includes 50 challenging sequences with full annotations that represent realistic tracking challenges, such as large deformations and severe occlusions. The experimental result of the proposed method shows considerable improvement in performance over the state-of-the-art tracking methods.

12.
Cell Physiol Biochem ; 38(4): 1544-52, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-27074047

RESUMO

BACKGROUND/AIMS: Emerging evidence shows that microRNAs (miRNAs) play a crucial role in the regulation of activation, proliferation and apoptosis of hepatic stellate cells (HSCs). Previous studies have indicated that miR-125a-5p is correlated with hepatitis B virus replication and disease progression. However, little is known about the biological role and underlying mechanism of miR-125a-5p in liver fibrosis. METHODS: We analyzed the level of miR-125a-5p in carbon tetrachloride-induced liver fibrosis and activated HSCs. We analyzed the effects of miR-125a-5p down-regulation on HSC activation and proliferation. We also analyzed the binding of miR-125a-5p to the 3'-untranslated region of factor inhibiting hypoxia-inducible factor 1 (FIH1) mRNA. RESULTS: Up-regulation of miR-125a-5p was observed in the liver tissues of fibrotic mice and activated HSCs. Down-regulation of miR-125a-5p prevented the activation and proliferation of HSCs. FIH1, a negative modulator of hypoxia inducible factor 1, was confirmed to be a target of miR-125a-5p using the luciferase reporter assay. Further studies demonstrated that miR-125a-5p prompted the activation and proliferation of HSCs, at least in part, by down-regulating FIH1. CONCLUSION: Our findings shed new light on miRNAs as a promising therapeutic target in liver fibrosis.


Assuntos
MicroRNAs/metabolismo , Oxigenases de Função Mista/metabolismo , Actinas/metabolismo , Animais , Tetracloreto de Carbono/toxicidade , Proliferação de Células , Células Cultivadas , Colágeno Tipo I/genética , Colágeno Tipo I/metabolismo , Modelos Animais de Doenças , Regulação para Baixo , Células HEK293 , Células Estreladas do Fígado/citologia , Células Estreladas do Fígado/metabolismo , Hepatócitos/citologia , Hepatócitos/metabolismo , Humanos , Cirrose Hepática/etiologia , Cirrose Hepática/metabolismo , Masculino , Camundongos , Camundongos Endogâmicos C57BL , MicroRNAs/antagonistas & inibidores , MicroRNAs/genética , Oxigenases de Função Mista/antagonistas & inibidores , Oxigenases de Função Mista/genética , Oligonucleotídeos/metabolismo , RNA Interferente Pequeno/metabolismo , Alinhamento de Sequência , Regulação para Cima
13.
Int J Clin Exp Pathol ; 7(8): 5051-6, 2014.
Artigo em Inglês | MEDLINE | ID: mdl-25197378

RESUMO

Cystic lymphangioma of the adrenal gland is a rare and benign lesion, most often found incidentally during abdominal imaging studies, abdominal surgery or at autopsy. We aimed to retrospectively review all adrenal lymphangioma cases at our hospital, further document their lymphatic origin by immunohistochemical staining and discuss the differential diagnosis with other cystic adrenal gland lesions. A total of 3 adrenal lymphangioma cases were identified. All three patients were men and adults at time of diagnosis aged 41 years, 43 years, and 66 years, respectively. All were incidentally identified during investigating for unrelated reasons, two of which were discovered by routine radiologic check-up while the last one was found during imaging detection of ureteral cancer. The average size of an adrenal lymphangioma lesion was 3.2 cm (range, 2.5-4.6 cm). Histologically, all three cases showed a typical multicystic architecture with dilated spaces lined by flattened, bland, simple lining. The cystic spaces occasionally contained proteinaceous material but lacked red blood cell content. On immunohistochemical stains, D2-40 cytoplasmic staining was positive in all three lesions, whereas AE1/AE3 was negative, thus, confirming their lymphatic nature.


Assuntos
Neoplasias das Glândulas Suprarrenais/patologia , Linfangioma Cístico/patologia , Adulto , Idoso , Biomarcadores Tumorais/análise , Diagnóstico Diferencial , Humanos , Imuno-Histoquímica , Masculino
14.
Hum Pathol ; 45(5): 1115-21, 2014 May.
Artigo em Inglês | MEDLINE | ID: mdl-24746217

RESUMO

Myopericytoma is a benign mesenchymal neoplasm thought to comprise part of a spectrum of perivascular myoid cell neoplasms with myofibroma, angioleiomyoma, and glomus tumor. We describe 2 such neoplasms involving the urinary tract: 1 incidentally identified in the kidney of a 59-year-old woman and 1 in the urinary bladder of a 52-year-old woman who presented with urinary frequency and dysuria. Histologically, the bladder tumor was composed of numerous blood vessels surrounded by plump perivascular myoid cells, as in subcutaneous myopericytoma. The renal tumor showed similar morphology centrally and a symplastic glomus tumor-like growth pattern at the periphery. Immunohistochemically, both tumors were reactive for markers of smooth muscle differentiation, such as smooth muscle actin and caldesmon/calponin but negative for CD34, cathepsin K, and S100 protein. Both patients are free of disease 14 and 39 months after resection, respectively. Our findings broaden the morphologic spectrum of myopericytoma.


Assuntos
Neoplasias Renais/diagnóstico , Neoplasias de Tecido Conjuntivo/diagnóstico , Neoplasias da Bexiga Urinária/diagnóstico , Proteínas de Ligação ao Cálcio/análise , Proteínas de Ligação a Calmodulina/análise , Diagnóstico Diferencial , Feminino , Humanos , Neoplasias Renais/patologia , Proteínas dos Microfilamentos/análise , Pessoa de Meia-Idade , Neoplasias de Tecido Conjuntivo/patologia , Pericitos/patologia , Neoplasias da Bexiga Urinária/patologia
16.
Zhonghua Yi Xue Za Zhi ; 85(16): 1133-6, 2005 Apr 27.
Artigo em Chinês | MEDLINE | ID: mdl-16029575

RESUMO

OBJECTIVE: To evaluate whether RNA interference can protect porcine endothelial cells from complement mediated cytotoxicity. METHODS: Immortalized porcine aortic endothelial cells of the line PED were cultured and transfected with alpha1,3-galactosyltransferase (alpha1, 3-GT) specific siRNAs. Cells transfected with mismatch SiRNA was used as negative controls. Forty-eight hours later the cells were collected. The expression of alpha1, 3-GT mRNA was examined by RT-PCR. The expression of alpha-Gal was examined by flow cytometry. PED cells ere labeled with (51)Cr and mixed with normal human serum (NHS). The release of (51)Cr was measured by gamma-ray counter. Heat inactivated NHS (HINHS) was used as control. RESULTS: Two isoforms (isoform 1 and isoform 2) were amplified from the PED cells. The expression of alpha1, 3-GT in the PED cells transfected with SiRNA-1 was lower by 70% in comparison with the mock group (69% for the isoform 1 and 72% for the isoform 2, both P < 0.05). However, the expression of alpha1, 3-GT in the PED cells transfected with SiRNA21 was not different from those in the mock group and mismatch group (both P > 0.05). Flow cytometry showed that the average fluorescence intensity of the PED cells transfected with SiRNA-1 was 52, 9, significantly lower than that of the mismatch group and mock group (493.9 and 5-5.7 respectively, both P < 0.0). Fluorescence microscopy observed the "silence effect" of alphaGal after SiRNA-1 transfection. Added with 20% and 40% NHS, the cell dissolution rate of the SiRNA transfection group was lower than that of the mock group by 70% and 60% respectively. CONCLUSIONS: alpha1, 3GT gene silencing actually occurs following transfection of SiRNA-1. Porcine endothelial cells can be the targets of RNAi.


Assuntos
Citotoxicidade Imunológica , Células Endoteliais/imunologia , Galactosiltransferases/biossíntese , Interferência de RNA/fisiologia , Animais , Aorta/citologia , Proteínas do Sistema Complemento/imunologia , Células Endoteliais/metabolismo , Galactosiltransferases/genética , Inativação Gênica , RNA Mensageiro/biossíntese , RNA Mensageiro/genética , RNA Interferente Pequeno/fisiologia , Suínos , Transfecção
17.
Artigo em Chinês | MEDLINE | ID: mdl-16038470

RESUMO

OBJECTIVE: To study whether the porcine endothelial cells (PECs) lines transfected by HLA-G1 can alter the lysis mediated by human peripheral blood mononuclear cell (PBMC) and natural killer cell 92 (NK-92). METHODS: By use of liposomes pack, the pcDNA3. 0 eukaryotic expression vector carrying HLA-G1 was transfected into PECs. Using indirect immunofluorescence and RT-PCR assays, the HLA-G1 expression in PECs was detected. The alteration of the lysis mediated by PBMC and NK-92 was detected by 51Cr-release assays. RESULTS: HLA-G1 expression could be detected in PECs after transfection of HLA-G1 at the levels of protein and RNA. It also could be found that the survival rate of transfected PECs was much higher than that of non-transfected PECs, when both of them faced the lysis mediated by human PBMC and NK-92. After transfecting the expression of HLA-G1 could be found in the transfected PECs and the lysis mediated by PBMC and NK-92 to PECs decreased obviously (P<0.05). CONCLUSION: The PECs transfected by HLA-G1 can decrease the NK lysis, so that it may provide us a new thought to inhibit the xeno-cell-rejection.


Assuntos
Células Endoteliais/imunologia , Células Endoteliais/transplante , Antígenos HLA/genética , Antígenos de Histocompatibilidade Classe I/genética , Transfecção/métodos , Animais , Linhagem Celular , Células Endoteliais/metabolismo , Técnica Indireta de Fluorescência para Anticorpo , Vetores Genéticos , Rejeição de Enxerto/imunologia , Antígenos HLA/imunologia , Antígenos HLA-G , Antígenos de Histocompatibilidade Classe I/imunologia , Humanos , Células Matadoras Naturais/imunologia , Monócitos/imunologia , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Suínos , Transplante Heterólogo
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