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1.
Environ Pollut ; 254(Pt B): 113053, 2019 Aug 14.
Artigo em Inglês | MEDLINE | ID: mdl-31465898

RESUMO

To investigate the removal characteristics of ammonium-nitrogen (NH4+-N), nitrite-nitrogen (NO2--N), nitrate-nitrogen (NO3--N), and total nitrogen from groundwater by a degradable composite active medium, kinetics, thermodynamics, and equilibrium adsorption, experiments were performed using scoria and degrading bacteria immobilized on scoria. Removal of NH4+-N, NO2--N, and NO3--N was conducted in adsorption experiments using different times, initial concentrations, pH values, and groundwater chemical compositions (Ca2+, Mg2+, HCO3-, CO32-, Fe2+, Mn2+, and SO42-). The results showed that the removal of nitrogen by the composite active medium was obviously better than that of scoria alone. The removal rates of NH4+-N (C0 = 5 mg/L), NO2--N (C0 = 5 mg/L), and NO3--N (C0 = 100 mg/L) by the composite active medium within 1 h were 96.05%, 82.40%, and 83.16%, respectively. The adsorption kinetics were well fitted to a pseudo-second order model, whereas the equilibrium adsorption agreed with the Freundlich model. With changes in the pH, variation in the removal could be attributed to the combined effect of hydrolysis and competitive ion adsorption, and the optimum pH was 7. Different concentration conditions, hardness, alkalinity, anions, and cations showed different promoting and inhibiting effects on the removal of nitrogen. A careful examination of ionic concentrations in adsorption batch experiments suggested that the sorption behavior of nitrogen onto the immobilized medium was mainly controlled by ion exchange. The degrading bacteria on the scoria surface were eluted and analyzed by metagenomic sequencing. There were significant differences in the number of operational taxons, relative abundances, and community diversity among degrading bacteria after adsorption of the three forms of nitrogen. The relative abundance of degrading bacteria was highest after NO3--N removal, and the diversity was highest after NO2--N removal. Pseudomonas and Serratia were the dominant genera that could efficiently remove NH4+-N and NO2--N.

2.
Zhongguo Yi Liao Qi Xie Za Zhi ; 43(4): 294-296, 2019 Jul 30.
Artigo em Chinês | MEDLINE | ID: mdl-31460725

RESUMO

There is no corresponding product standard and industry standard for the search and development of magnetocardiograph at home and abroad. In this paper, based on the working principle and clinical application function of magnetocardiograph, and on the basis of relevant regulations and normative documents of medical device registration, some thoughts and concerns about the technical review of this kind of product are put forward.

3.
Langmuir ; 35(32): 10405-10411, 2019 Aug 13.
Artigo em Inglês | MEDLINE | ID: mdl-31337217

RESUMO

A new separation-based analytical method was developed to evaluate the multivalency and cooperativity of supramolecular host-guest complexation in porous materials. The method is based on inverse mixed-mode chromatography in which a porous material with a multivalent functional group is packed into a column and bound with a complementary guest molecule to form a multivalent complex. The bound guest molecules are eluted in the mobile phase and detected by appropriate methods such as UV absorption. The retention factor of the guest molecule is determined and broken down into the contributions of noncovalent interactions between binding sites (e.g., hydrophobic and ionic components), thereby calculating the effective molarity and cooperativity factor of the complexation. Two model systems denoted as RP/SCX and RP/SAX were analyzed by the established method. On average, the RP/SCX system has an effective molarity (EM) of 0.14 M and a cooperativity factor (ß) of 0.86, while the RP/SAX system has an EM value of 0.18 M and a ß value of 2.3. Interestingly, experiments have shown that these values do not change with changes in the intrinsic binding strength of the constituent sites. In summary, the developed method allows for quantitative assessment of multivalency and cooperativity effects in porous materials, providing a valuable complement to the analytical toolbox for supramolecular chemists and materials scientists.

5.
Zhonghua Wei Zhong Bing Ji Jiu Yi Xue ; 31(5): 556-561, 2019 May.
Artigo em Chinês | MEDLINE | ID: mdl-31198139

RESUMO

OBJECTIVE: To explore the impacts of clinical pulmonary infection score (CPIS) on duration and defined daily doses (DDDs) of antibiotics in patients with bacterial severe pneumonia in intensive care unit (ICU). METHODS: Patients with severe pneumonia, whose antibiotic usage was prescribed with the guide of CPIS, and admitted to ICU severe respiratory and infectious disease ward of Guizhou Medical University Affiliated Hospital from May 2017 to October 2017 were enrolled as CPIS group. Patients with the first CPIS score > 5 were given antimicrobial therapy, and the score was dynamically evaluated every 2-3 days. If the CPIS score < 5, the score was evaluated again after 2 days. If the score was still < 5, the antimicrobial drugs were discontinued. Patients admitted to the same ward from November 2016 to April 2017 were regarded as controls, of whom the antibiotic usage was completely conducted by the clinical experience of the chief physician. The duration and DDDs of antibiotics were compared between patients in two groups. At the same time, the usage of ventilator and prognostic indicators (the length of ICU stay, ICU mortality) were recorded. Kaplan-Meier survival curve was drawn, and the cumulative survival rates of 28 days, 90 days and 12 months were analyzed and compared between the two groups. RESULTS: In our department, 177 and 182 patients were admitted to ICU from November 2016 to April 2017 and from May 2017 to October 2017, respectively, of whom 101 and 65 patients with severe pneumonia were collected respectively during the two stages. There was no significant difference in gender composition, age, underlying diseases, vital signs, acute physiology and chronic health evaluation II (APACHE II) score, or peripheral blood routine at admission between the two groups, indicating that the baseline data of the two groups were equally comparable. During the treatment process, there was no significant difference in the duration of mechanical ventilation [hours: 126.0 (69.0, 228.8) vs. 120.0 (72.0, 192.0)], the length of ICU stay [days: 7.0 (5.0, 11.0) vs. 8.0 (5.0, 14.0)], or ICU mortality [18.8% (19/101) vs. 26.2% (17/65)] between the control group and CPIS group (all P > 0.05). Kaplan-Meier survival curve analysis showed that there was no significant difference in the cumulative survival rate of 28 days (log-rank test: χ2 = 0.540, P = 0.462), 90 days (log-rank test: χ2 = 0.332, P = 0.564) or 12 months (log-rank test: χ2 = 0.833, P = 0.362). Patients from CPIS guided group, however, had a shorter duration of antibiotics usage (days: 7.54±4.81 vs. 9.88±4.96, P < 0.01), and had a lower DDDs of antibiotics (17.58±13.09 vs. 22.73±18.31, P < 0.05) as compared with those in the control group. CONCLUSIONS: CPIS-guided therapeutic regimen shortens antibiotic duration and decreases antibiotic DDDs in patients with severe pneumonia in ICU, indicating the values of CPIS in guiding antibiotics usage in these patients.


Assuntos
Antibacterianos/uso terapêutico , Pneumonia Bacteriana/tratamento farmacológico , Índice de Gravidade de Doença , Feminino , Humanos , Unidades de Terapia Intensiva , Masculino , Guias de Prática Clínica como Assunto , Resultado do Tratamento
6.
J Sep Sci ; 42(17): 2796-2804, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31222942

RESUMO

A method for the preparation of novel mixed-mode reversed-phase/strong cation exchange stationary phase for the separation of fixed-dose combination drugs has been developed. An epoxysilane bonded silica prepared by vapor phase deposition was used as a starting material to produce diol, octadecyl, sulfonate, and mixed octadecyl/sulfonate groups bonded silica phases. The chemical structure and surface coverage of the functional groups on these synthesized phases were confirmed by fourier-transform infrared and solid-state 13 C NMR spectroscopy and elemental analysis. Alkylbenzene homologs, basic drugs, nucleobases and alkylaniline homologs were used as probes to demonstrate the reversed-phase, ion exchange, hydrophilic interaction and mixed-mode retention behaviors of these stationary phases. The octadecyl/sulfonate bonded silica exhibits pronounced mixed-mode retention behavior and superior retentivity and selectivity for alkylaniline homologs. The mixed-mode retention is affected by either ionic or solvent strength in the mobile phase, permiting optimization of a separation by fine tuning these parameters. The mixed-mode stationary phase was applied to separate two fixed-dose combination drugs: compound reserpine tablets and compound methoxyphenamine capsules. The results show that simultaneous separation of multiple substances in the compound dosage can be achieved on the mixed-mode phase, which makes multi-cycles of analysis for multiple components obsolete.

7.
Artigo em Inglês | MEDLINE | ID: mdl-31123823

RESUMO

Neurological soft signs (NSS) and neurocognitive deficits (ND) are highly prevalent in schizophrenia, and have been separately proposed as candidate endophenotypes of schizophrenia. However, few relevant studies focus on remitted patients with schizophrenia (RP) and integrate NSS and ND as a composite endophenotype. This study aimed to explore the NSS and ND and examine the comparative relationship between them in RP, their first-degree unaffected relatives (FDR), and healthy controls, furthermore, to seek potential endophenotypes subitems of NSS and ND and create a composite endophenotype. 86 RP, 86 FDR, and 86 healthy controls were included. NSS and ND were independently assessed with Cambridge Neurological Inventory and MATRICSTM Consensus Cognitive Battery. RP had more NSS and ND than FDR in all subitems except disinhibition, information processing speed, working memory, and visual memory. Similarly, FDR presented poorer performance than controls in all subscales except disinhibition, sensory integration, working memory, and visual memory. Six subitems of NSS and ND met the criteria of endophenotype and the three groups were most accurately classified (71.2%) with these subitems working as a composite endophenotype. Moreover, information processing speed, attention, and social cognition were associated with sensory integration in RP and FDR. These findings add evidences that certain subitems of NSS and ND might be the endophenotypes of schizophrenia and integrating these endophenotypes may prove useful in identifying schizophrenia and high-risk individuals. Furthermore, sensory integration and specific cognitive domains covary, hence suggesting an overlap of compromised underlying neural systems.

8.
Math Biosci Eng ; 16(3): 1471-1488, 2019 02 21.
Artigo em Inglês | MEDLINE | ID: mdl-30947429

RESUMO

In this work, dynamical behaviors of discrete time Beverton-Holt population model with fuzzy parameters are studied. It provides a flexible model to fit population data. For three different fuzzy parameters and fuzzy initial conditions, according to a generalization of division (g-division) of fuzzy number, it can represent dynamical behaviors including boundedness, global asymptotical stability and persistence of positive solution. Finally, two examples are given to demonstrate the effectiveness of the results obtained.


Assuntos
Biologia Computacional/métodos , Lógica Fuzzy , Dinâmica Populacional , Algoritmos , Animais , Modelos Biológicos , Probabilidade , Reprodução
9.
J Cardiothorac Surg ; 14(1): 47, 2019 Feb 28.
Artigo em Inglês | MEDLINE | ID: mdl-30819225

RESUMO

BACKGROUND: Obstruction of the left or right coronary artery is a rare but lethal complication during transcatheter aortic valve implantation (TAVI). The new J-Valve™ prosthesis is a new second generation TAVI device which has several features to avoid the coronary obstruction such as low profile design and clip fixation of the native leaflets. The aim of this study is to report our initial experience of using this valve in treating patient with high risk factors for coronary obstruction during TAVI procedure. CASE PRESENTATION: Three high surgical risk patients (All females with 77, 76, and 75 years old) with symptomatic aortic stenosis were enrolled. All patients have the common feature of low coronary ostium height (< 10 mm) with narrowed aortic sinus (< 30 mm) on CT angiogram and marked leaflet calcification. Three 25 mm J-Valve prostheses were successfully implanted through trans-apical approach. No coronary obstruction was noted for these patients. Effective aortic open area was significantly increased after valve implantation (Preoperative 0.7, 0.7 and 0.65 cm2 - Postoperative 1.8, 1.9 and 2.0 cm2). Only one patient was noted to have trivial degree paravalvular leakage. CONCLUSION: The new J-Valve prosthesis is a new second generation TAVI device. This system may provide another safety treatment option for patient with high risk factor for coronary obstruction underwent TAVI procedure.


Assuntos
Estenose da Valva Aórtica/cirurgia , Valva Aórtica/cirurgia , Oclusão Coronária/prevenção & controle , Próteses Valvulares Cardíacas , Substituição da Valva Aórtica Transcateter/instrumentação , Idoso , Idoso de 80 Anos ou mais , Oclusão Coronária/etiologia , Feminino , Humanos , Desenho de Prótese , Fatores de Risco , Substituição da Valva Aórtica Transcateter/efeitos adversos , Substituição da Valva Aórtica Transcateter/métodos
10.
Zhonghua Wei Zhong Bing Ji Jiu Yi Xue ; 31(2): 214-220, 2019 Feb.
Artigo em Chinês | MEDLINE | ID: mdl-30827312

RESUMO

OBJECTIVE: To systematically analyze the effect of haemoperfusion (HP) combined with continuous veno-veno haemofiltration (CVVH) in the treatment of the patients with paraquat poisoning (PQP). METHODS: Words of paraquat, poisoning, continuous venous hemofiltration, hemoperfusion, hemodiafiltration in Chinese and paraquat, poisoning, intoxication, haemofiltration, continuous venovenous haemofiltration, haemoperfusion in English were chosen as keywords, the Chinese and English literatures about acute PQP treated with HP combined with CVVH published in Wanfang database, CNKI, CBM, VIP database, PubMed, Embase, Cochrane Library were searched by computer, and the retrieval time was from the establishment of the database to July 2018. The experimental group was treated with HP combined with CVVH, while the control group was treated with HP alone. Besides, the outcome indicators included mortality, survival time of dead patients (the patient's time from exposure to poison to death), serum creatinine (SCr), alanine aminotransferase (ALT), arterial partial pressure of oxygen (PaO2), and incidence of circulatory and respiratory failure. The literature data were extracted by two researchers independently, the quality of the literature was evaluated according to the modified Jadad score table or Newcastle-Ottawa scale (NOS), and the Meta-analysis was carried out by RevMan 5.3 software; and the stability of the results of Meta-analysis was tested by sensitivity analysis. Further, the publication bias was analyzed through drawing a funnel diagram. RESULTS: Finally, 20 articles were included, with 18 in Chinese and 2 in English. Among them, 6 were randomized controlled trial (RCT) and 14 were case-control studies. Furthermore, a total of 2 870 patients were involved, with 1 558 in the control group and 1 312 in the experimental group. Meta-analysis showed that the mortality rate of patients in the experimental group was significantly lower than that in the control group [odds ratio (OR) = 0.55, 95% confidence interval (95%CI) = 0.42 to 0.73, P < 0.000 1], the patients' time from toxin exposure to death was significantly longer than that in the control group [standard mean difference (SMD) = 2.16, 95%CI = 1.46 to 2.86, P < 0.000 01). In the course of treatment, the peak value of SCr in the experimental group was significantly lower than that in the control group (SMD = -0.53, 95%CI = -0.65 to -0.42, P < 0.000 01), and the peak value of ALT was also decreased (SMD = -0.72, 95%CI = -0.99 to -0.44, P < 0.000 01). Besides, there was no significant difference in PaO2 between the two groups on the 3rd day of treatment (SMD = 0.15, 95%CI = -0.19-0.49, P = 0.40), but on the 7th day, PaO2 in the experimental group was significantly higher than that in the control group (SMD = 0.23, 95%CI = 0.29 to 0.98, P = 0.000 3). Furthermore, the incidence of circulatory failure in the experimental group was significantly lower than that in the control group (OR = 0.26, 95%CI = 0.19 to 0.37, P < 0.000 01), but the incidence of respiratory failure was significantly higher than that in the control group (OR = 4.14, 95%CI = 3.00 to 5.72, P < 0.000 01). The influence of heterogeneity on statistical results was excluded in the sensitivity analysis, and funnel plot diagram was applied to indicate the publication bias of mortality and survival time of the dead patients. CONCLUSIONS: Combined with HP alone, HP combined with CVVH could better improve liver and kidney function and oxygenation state of PQP patients, reduce the incidence of early circulatory failure, prolong the survival time and reduce the death rate of PQP patients.


Assuntos
Hemofiltração/métodos , Hemoperfusão/métodos , Paraquat/envenenamento , Humanos , Ensaios Clínicos Controlados Aleatórios como Assunto , Resultado do Tratamento
11.
Nature ; 567(7747): 234-238, 2019 03.
Artigo em Inglês | MEDLINE | ID: mdl-30814736

RESUMO

Longitudinal bone growth in children is sustained by growth plates, narrow discs of cartilage that provide a continuous supply of chondrocytes for endochondral ossification1. However, it remains unknown how this supply is maintained throughout childhood growth. Chondroprogenitors in the resting zone are thought to be gradually consumed as they supply cells for longitudinal growth1,2, but this model has never been proved. Here, using clonal genetic tracing with multicolour reporters and functional perturbations, we demonstrate that longitudinal growth during the fetal and neonatal periods involves depletion of chondroprogenitors, whereas later in life, coinciding with the formation of the secondary ossification centre, chondroprogenitors acquire the capacity for self-renewal, resulting in the formation of large, stable monoclonal columns of chondrocytes. Simultaneously, chondroprogenitors begin to express stem cell markers and undergo symmetric cell division. Regulation of the pool of self-renewing progenitors involves the hedgehog and mammalian target of rapamycin complex 1 (mTORC1) signalling pathways. Our findings indicate that a stem cell niche develops postnatally in the epiphyseal growth plate, which provides a continuous supply of chondrocytes over a prolonged period.

12.
J Affect Disord ; 246: 861-866, 2019 03 01.
Artigo em Inglês | MEDLINE | ID: mdl-30795492

RESUMO

BACKGROUND: Risperidone, an atypical antipsychotic medication, is recommended as a first line treatment for acute mania in patients with bipolar disorder I (BD I). It is unknown if continuing treatment with risperidone adjunct to lithium or valproate after remission of the manic episode offers additional benefit in prevention of mood episode relapse. METHODS: A post-hoc subgroup analysis was conducted using data from a 52-week, double-blind, placebo controlled trial involving 93 patients treated with oral risperidone adjunct to mood stabilizer, randomized to arms discontinuing risperidone at entry ("0-week arm"), 24 weeks after entry ("24-week arm") or continuing risperidone ("52-week arm"). Time to any episode, manic episode, and depressive episode was compared between arms using Cox regression models. RESULTS: Time to any mood episode was longer in the 24-week arm versus the 0-week arm (HR: 0.57, 95% CI: 0.31-1.05, P = 0.07) and shorter in the 52-week arm versus 24-week arm (HR: 1.85, 95% CI: 1.00-3.41, P = 0.05), though these results were not significant. Time to relapse into manic episode was significantly longer in the 24-week arm versus 0-week arm (HR: 0.14, 95% CI: 0.03, 0.65, P = 0.01). No other significant differences were observed between arms. LIMITATIONS: The sample size was modest, as the original dataset was powered to study optimal duration for two atypical antipsychotics. CONCLUSIONS: Adjunctive risperidone treatment was observed to reduce the risk of manic episodes during the first 24 weeks, but not after 24 weeks. Treatment did not appear to reduce the risk of depressive episodes.


Assuntos
Antipsicóticos/uso terapêutico , Transtorno Bipolar/prevenção & controle , Lítio/uso terapêutico , Risperidona/uso terapêutico , Prevenção Secundária/métodos , Ácido Valproico/uso terapêutico , Administração Oral , Adolescente , Adulto , Idoso , Transtorno Bipolar/tratamento farmacológico , Método Duplo-Cego , Esquema de Medicação , Quimioterapia Combinada , Feminino , Seguimentos , Humanos , Masculino , Pessoa de Meia-Idade , Resultado do Tratamento , Adulto Jovem
13.
Zhonghua Wei Zhong Bing Ji Jiu Yi Xue ; 31(1): 44-49, 2019 Jan.
Artigo em Chinês | MEDLINE | ID: mdl-30707868

RESUMO

OBJECTIVE: To compare the influence of sevoflurane inhalation sedation and propofol intravenous sedation on duration of endotracheal intubation as well as the length of intensive care unit (ICU) stay and total length of hospital stay in postoperative critical patients. METHODS: Six databases including CNKI, Wanfang data, PubMed, Embase, Cochrane Library and Web of Science were searched for randomized controlled trials (RCTs) about the influence of sevoflurane inhalation sedation or propofol intravenous sedation on the sedation time, the duration of endotracheal intubation, the length of ICU stay, the total length of hospital stay and the adverse effects rate in postoperative critical patients from the time of database establishment to July 2018. At the same time, the reference materials of included literature were retrieved manually. All literatures were screened by three independent reviewers, and the data extraction and quality evaluation of the included studies were conducted. Meta-analysis was used for RCT that met the quality standards. RESULTS: A total of 7 RCT studies were enrolled involving 537 patients who were all transferred into ICU after surgery with trachea cannula. Among the patients, 272 received sevoflurane sedation while the other 265 received propofol sedation. All the included studies were well designed and of high quality. The results of Meta-analysis showed that compared with propofol sedation, sevoflurane sedation could significantly shorten the duration of endotracheal intubation [standardized mean difference (SMD) = -0.60, 95% confidence interval (95%CI) = -0.88 to -0.31, P < 0.000 1] and the total length of hospital stay (SMD = -0.36, 95%CI = -0.61 to -0.12, P = 0.003), and lower the cardiac troponin T (cTnT) within 12-24 hours after ICU admission (SMD = -0.61, 95%CI = -0.85 to -0.36, P < 0.000 01). There was no significant difference in the sedation time (SMD = -0.07, 95%CI = -0.29 to 0.15, P = 0.52), the length of ICU stay (SMD = -0.19, 95%CI = -0.39 to 0.01, P = 0.06), the incidence of nausea and vomiting [odds ratio (OR) = 1.19, 95%CI = 0.61 to 2.32, P = 0.61] or incidence of delirium (OR = 0.80, 95%CI = 0.34 to 1.90, P = 0.62) between sevoflurane group and propofol group. CONCLUSIONS: Sevoflurane inhalation sedation may lead to shorter duration of endotracheal intubation and total length of hospital stay, and had better protection for myocardium as compared with propofol intravenous sedation. The above conclusions needed further study to confirm, due to the lack of literature enrolled in this Meta-analysis.


Assuntos
Anestésicos Inalatórios , Intubação Intratraqueal/estatística & dados numéricos , Tempo de Internação/estatística & dados numéricos , Sevoflurano/administração & dosagem , Anestésicos Intravenosos , Cuidados Críticos , Humanos , Propofol/administração & dosagem , Ensaios Clínicos Controlados Aleatórios como Assunto , Procedimentos Cirúrgicos Operatórios
14.
J Vasc Access ; 20(5): 524-529, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-30596472

RESUMO

INTRODUCTION: Ultrasound-guided venipuncture and tip location by intracavitary electrocardiogram have many advantages during the insertion of peripherally inserted central catheters, both in terms of safety and cost-effectiveness. Recently, a new tip-conductive peripherally inserted central catheters and new Doppler ultrasound device integrated with intracavitary electrocardiogram have been introduced into clinical practice in China. A randomized multicenter study (clinical trial no. NCT03230357) was performed to verify the feasibility and accuracy of intracavitary electrocardiogram, as performed with this new peripherally inserted central catheters and device. METHODS: Our study enrolled a total of 2250 adult patients in 10 different Chinese hospitals. The patients were randomly assigned to either the study group (intracavitary electrocardiogram) or the control group (anatomical landmark guidance) in a 2:1 allocation. Ultrasound was used in both groups for venipuncture and tip navigation. All patients underwent chest X-ray after the procedure to verify the position of the catheter tip. RESULTS: No insertion-related complications were reported in either group. In the study group, first-attempt successful tip location was 91.7% (95% confidence interval: 90.3%-93.1%), significantly higher than 78.9% (95% confidence interval: 76.0%-81.9%) observed in the control group (p < 0.001). As evaluated by post-procedural chest X-ray, tip location in the study group had a sensitivity of 99.3% (95% confidence interval: 98.8%-99.7%), significantly higher than 86.8% (95% confidence interval: 84.4%-89.2%) observed in the anatomical landmark group (p < 0.001). CONCLUSION: These results indicated that during peripherally inserted central catheters insertion in adult patients, tip location with intracavitary electrocardiogram guidance, as carried out by a new tip-conductive peripherally inserted central catheters and intracavitary electrocardiogram integrated ultrasound device, was more effective and more accurate than tip location using anatomical landmarks.


Assuntos
Cateterismo Periférico/instrumentação , Cateteres de Demora , Pressão Venosa Central , Eletrocardiografia/instrumentação , Transdutores , Ultrassonografia Doppler/instrumentação , Ultrassonografia de Intervenção/instrumentação , Idoso , Pontos de Referência Anatômicos , Cateterismo Periférico/efeitos adversos , China , Eletrocardiografia/métodos , Desenho de Equipamento , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Valor Preditivo dos Testes , Estudos Prospectivos
15.
Int J Mol Med ; 43(1): 221-232, 2019 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-30431067

RESUMO

Acupuncture is a traditional Chinese medicinal therapy, which is used for the amelioration of cognitive dysfunction. The aim of this study was to investigate the effectiveness and relevancy mechanisms of 'governor vessel­unblocking and mind­regulating' acupuncture therapy for cognitive dysfunction in rats with ischemia. For this purpose, we used the middle cerebral artery occlusion (MCAO) method to induce cognitive dysfunction in rats. The behavioral changes in the rats were examined using the Morris water maze (MWM) test. The effects of the treatment on oxidative stress response and the function of the mitochondria in brain tissues were also assessed. The results revealed that 'governor vessel­unblocking and mind­regulating' acupuncture therapy markedly improved the cognitive ability of the rats with cognitive dysfunction. The production of pro­oxidative stress factors, including nitric oxide (NO) and inducible nitric oxide synthase (iNOS), was also blocked along with the amelioration of cognitive function, while the production of adenosine triphosphate (ATP), superoxide dismutase (SOD) and cyclooxygenase (COX) was restored. At the molecular level, the accumulation of amyloid ß (Aß) in the mitochondria was suppressed by 'governor vessel­unblocking and mind­regulating' acupuncture therapy, which may be attributed to the inhibition of the function of translocase of outer mitochondrial membrane 40 (TOMM40) and translocase of inner mitochondrial membrane 17A (TIMM17A). On the whole, the findings of the present study confirm the effects of 'governor vessel­unblocking and mind­regulating' acupuncture therapy on cognitive dysfunction induced by brain ischemia in rats, and that the mechanisms underlying the effects of this treatment might be mediated through the inhibition of TOMM40 and TIMM17A synthesis, which can relieve mitochondrial dysfunction from the accumulation of Aß.


Assuntos
Terapia por Acupuntura , Disfunção Cognitiva/terapia , Infarto da Artéria Cerebral Média/terapia , Peptídeos beta-Amiloides/metabolismo , Animais , Encéfalo/patologia , Disfunção Cognitiva/patologia , Disfunção Cognitiva/fisiopatologia , Modelos Animais de Doenças , Regulação para Baixo , Infarto da Artéria Cerebral Média/patologia , Infarto da Artéria Cerebral Média/fisiopatologia , Potencial da Membrana Mitocondrial , Memória , Mitocôndrias/metabolismo , Mitocôndrias/patologia , Proteínas Mitocondriais/metabolismo , Estresse Oxidativo , Prostaglandina-Endoperóxido Sintases/metabolismo , Ratos Sprague-Dawley
16.
Ann Am Thorac Soc ; 16(4): 478-487, 2019 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-30562038

RESUMO

RATIONALE: Respiratory muscle strength in children can be assessed by maximal inspiratory pressures (MIP), maximal expiratory pressures (MEP), and sniff nasal inspiratory pressures (SNIP). However, previous studies involved small cohorts of healthy children and reported wide reference ranges. OBJECTIVES: To perform a systematic review to summarize existing reference ranges for MIP, MEP, and SNIP tests in healthy children and to conduct a meta-analysis to develop comprehensive prediction equations. DATA SOURCES: Five databases were searched for relevant studies from database inception to May 29, 2017. DATA EXTRACTION: Study inclusion was limited to publications that evaluated MIP, MEP, and SNIP values in healthy children aged 18 years or younger. Studies were also excluded if testing methodology differed greatly from the 2002 American Thoracic Society Statement on Respiratory Muscle Testing. Requests for raw data were made to authors via e-mail. SYNTHESIS: A total of 18 studies including 3,509 children were systematically reviewed. Diagnostic accuracy of the included studies was assessed using the QUADAS-2 tool, which revealed a high risk of bias for flow and timing and for applicability that may influence the generalizability of our findings. All 18 studies evaluated respiratory pressures in children in seated position. MIP tests were conducted from residual volume, MEP tests from total lung capacity, and SNIP tests from functional residual capacity. The MIP and MEP values in three age groups for boys and girls were summarized using meta-analysis based on individual participant data from five studies containing 1,709 healthy children. Further analyses showed that MIP and MEP were significantly greater in boys than in girls (P < 0.0001). In both sexes, MEP values were always greater than MIP values (P < 0.05). Multivariable random effects models were then performed to establish sex-specific prediction equations. These equations found age, height, and weight to be significant predictor variables. Only two studies with SNIP values from healthy children were included in the review, but they were not part of the meta-analysis. CONCLUSIONS: We summarized the available reference ranges for MIP, MEP, and SNIP tests based on existing literature, especially for three age groups, and developed prediction equations that can be used in pulmonary function laboratories to aid clinicians. Existing literature on SNIP tests is limited, and future studies are encouraged to explore their use in children. Systematic review registered with the International Prospective Register of Systematic Reviews (PROSPERO; CRD42017072004).

17.
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi ; 34(11): 1015-1020, 2018 Nov.
Artigo em Chinês | MEDLINE | ID: mdl-30591110

RESUMO

Objective To observe the effect of miR-27a-3p on bleomycin A5-induced pulmonary fibrosis (PF) in rats and explore the underlying mechanism. Methods Forty-five male SD rats were randomly divided into control group, miR-27a-3p agomir group and miR-27a-3p antagomir group. Each group contained 15 animals. All rats were injected intratracheally with bleomycin A5 to establish PF models. On the first day after bleomycin A5 administration, the rats in the control group, miR-27a-3p agomir group and miR-27a-3p antagomir group were injected at the caudal vein with physiological saline, agomir and antagomir, respectively. Injection was given one time each three days, totally nine times. On day 28, blood samples were collected and then underwent enzyme linked immunosorbent assay for procollagen type 1 carboxyterminal propeptide (P1CP) and procollagen type 3 aminoterminal propeptide (P3NP) concentrations. Subsequently, all rats were sacrificed to remove pulmonary tissue. Both HE and Masson staining were performed to evaluate the pathological changes of PF. The expression of miR-27a-3p, collagen type 1 (Col1), and collagen type 3 (Col3) were detected using fluorescence real time quantitative PCR. Western blotting was used to examine Col1, Col3, Wnt3a and ß-catenin levels. Results The miR-27a-3p agomir markedly increased miR-27a-3p expression in the pulmonary tissue, whereas its antagomir decreased it, showing higher transfection efficacy. The pulmonary inflammation and fibrosis degree was alleviated in the miR-27a-3p agomir group while aggravated in the miR-27a-3p antagomir group. In comparison with control group, serum P1CP and P3NP levels decreased in the miR-27a-3p agomir group but increased in the miR-27a-3p antagomir group. Treatment with miR-27a-3p agomir down-regulated the expression of Col1, Col3, Wnt3a and ß-catenin in the pulmonary tissue, while miR-27a-3p antagomir up-regulated their expression. Conclusion The miR-27a-3p inhibits the Wnt3a/ß-catenin signaling pathway, leading to the down-regulation of Col1 and Col3 expression and the subsequent alleviation of PF.


Assuntos
MicroRNAs/genética , Fibrose Pulmonar/tratamento farmacológico , Via de Sinalização Wnt , Animais , Colágeno Tipo I/metabolismo , Colágeno Tipo III/metabolismo , Masculino , Pró-Colágeno/metabolismo , Distribuição Aleatória , Ratos , Ratos Sprague-Dawley , Proteína Wnt3/metabolismo , beta Catenina/metabolismo
18.
BMC Surg ; 18(1): 100, 2018 Nov 16.
Artigo em Inglês | MEDLINE | ID: mdl-30445948

RESUMO

BACKGROUND: Thoracic endovascular aortic repair (TEVAR) is the therapeutic choice for type B aortic dissection. One of the most unfavored complications of this procedure is hemorrhage, which has a low incidence but high mortality. Renal hemorrhage (RH) after endovascular aortic repair has been rarely reported. We presented two cases of unexpected RH after TEVAR for complicated type B aortic dissection, and the potential causes, diagnosis and therapeutic management were discussed. CASE PRESENTATION: A 67-year-old female developed hypotension and progressively decrease of hemoglobin within 5 h after TEVAR for acute complicated type B dissection. Bedside ultrasonography and abdominal computed tomography angiography revealed a massive right perinephric hematoma. The right renal angiography detected multiple tortuous vascular branches with diffuse perinephric bleeding. The main trunk of right renal artery was embolized. The patient recovered uneventfully and presented with normal renal function 6 months later. Another patient was a 69-year-old male who was admitted for endovascular repair of a chronic complicated type B aortic dissection. The patient presented with hemodynamic instability early after TEVAR. Bedside ultrasonography showed a giant left retroperitoneal hematoma. The abdominal angiography revealed two active bleeding sits located in the distal branches of left renal artery. A super-selective embolization of the two arteries was performed, however the patient developed abdominal compartment syndrome and died of multiple organ failure. CONCLUSIONS: Unexpected RH after endovascular repair of aortic dissection might be associated with iatrogenic and idiopathic factors. Close surveillance and clinician's awareness of this rare complication is crucial for accurate and prompt diagnosis. Renal angiography and subsequent selective embolization of bleeding vessels are effective interventions for treating this fatal condition.

20.
Exp Lung Res ; : 1-11, 2018 Nov 19.
Artigo em Inglês | MEDLINE | ID: mdl-30449218

RESUMO

Purpose/aim: Activated coagulation and reduced fibrinolysis in alveolar compartment are an important characteristics in acute respiratory distress syndrome (ARDS). Alveolar epithelial cell type II (AECII) participates in regulating the intra-alveolar abnormalities of coagulation and fibrinolysis mainly through adjusting the productions of tissue factor (TF), plasminogen activator inhibitor (PAI)-1 and activated protein C (APC) in ARDS. NF-κB signal pathway may be involved in coagulation regulation in sepsis-induced ALI. The purpose of this study was to testify the hypothesis that NF-κB p65 (p65) knock-down would improve the abnormalities of coagulation and fibrinolysis mediated by lipopolysaccharide (LPS) stimulation in AECII. MATERIALS AND METHODS: p65 gene knock-down in AECII was achieved by small interfering RNA (siRNA) transfection. Rat AECII (RLE-6TN) with or without p65 gene knock-down were stimulated by LPS for 24 hours. And then cytolysate was used for TF, PAI-1 expression examination, and supernatant was collected for TF, PAI-1 and PC concentrations determination. Activation of NF-κB canonical pathway was simultaneously checked by western-blotting, RT-PCR and immunofluorescence respectively. RESULTS: TF, PAI-1 expressions in normal cells obviously increased under LPS stimulation with NF-κB canonical pathway activation represented by high levels of p65, p-p65, p-IκB with increased nuclear translocation of p-p65. Cells with NF-κB p65 knock-down, however, showed significant decreases in TF, PAI-1, p65, p-p65, p-IκB expressions following LPS stimulation with significant reduction in p-p65 nuclear translocation as compared to normal and siRNA control cells. The high concentrations of TF, PAI-1 and low level of APC in supernatant induced by LPS in normal cells were significantly reversed through p65 knock-down. CONCLUSIONS: The experimental findings demonstrate that NF-kB signaling pathway is involved in regulating the expressions of coagulation and fibrinolysis factors in LPS-stimulated AECII, which suggest that NF-kB signaling pathway may be a new target to correct intra-alveolar coagulation and fibrinolytic abnormalities in ARDS.

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