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1.
Eur J Radiol ; 134: 109466, 2021 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-33307459

RESUMO

PURPOSE: Accurate glioma grading and IDH mutation status prediction are critically essential for individualized preoperative treatment decisions. This study aims to compare the diagnostic performance of magnetic resonance (MR) amide proton transfer (APT) and diffusion kurtosis imaging (DKI) in glioma grading and IDH mutation status prediction. METHOD: Fifty-one glioma patients without treatment were retrospectively included. APT-weighted (APTw) effect and DKI indices, including mean diffusivity (MD), fractional anisotropy (FA), mean kurtosis (MK), and kurtosis FA (KFA) were obtained from APT and diffusion-weighted images, respectively. DKI indices in tumors were normalized to that in contralateral normal appearing white matter (CNAWM) and the APTw difference (ΔAPTw) between the two regions was calculated. Student's t-test, one-way ANOVA and ROC analyses were conducted. RESULTS: Among the enrolled 51 patients, 13 had glioma-II, 17 had glioma-III and 21 had glioma-IV. 25 patients were diagnosed as IDH-mutant, and 26 as IDH-wild type. MD and MK differed significantly between glioma-IV and glioma II/III (P < 0.05), but not between glioma-II and glioma-III. FA and KFA showed no significant difference among the three groups (P > 0.05). IDH-mutant group exhibited significantly higher MD and lower FA, MK and ΔAPTw than IDH-wild type (P < 0.05), whereas the two groups showed comparable KFA values. In contrast, ΔAPTw differed significantly across tumor grades and IDH mutation status (P < 0.05), with consistently better discriminatory performance than DKI indices in glioma grading and IDH mutation status prediction. CONCLUSIONS: APT imaging was superior to DKI in glioma grading and IDH mutation status prediction, benefiting accurate diagnoses and treatment decisions.

2.
PLoS One ; 15(12): e0244131, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33370368

RESUMO

INTRODUCTION: A large proportion of patients with COVID-19 develop acute kidney injury (AKI). While the most severe of these cases require renal replacement therapy (RRT), little is known about their clinical course. METHODS: We describe the clinical characteristics of COVID-19 patients in the ICU with AKI requiring RRT at an academic medical center in New York City and followed patients for outcomes of death and renal recovery using time-to-event analyses. RESULTS: Our cohort of 115 patients represented 23% of all ICU admissions at our center, with a peak prevalence of 29%. Patients were followed for a median of 29 days (2542 total patient-RRT-days; median 54 days for survivors). Mechanical ventilation and vasopressor use were common (99% and 84%, respectively), and the median Sequential Organ Function Assessment (SOFA) score was 14. By the end of follow-up 51% died, 41% recovered kidney function (84% of survivors), and 8% still needed RRT (survival probability at 60 days: 0.46 [95% CI: 0.36-0.56])). In an adjusted Cox model, coronary artery disease and chronic obstructive pulmonary disease were associated with increased mortality (HRs: 3.99 [95% CI 1.46-10.90] and 3.10 [95% CI 1.25-7.66]) as were angiotensin-converting-enzyme inhibitors (HR 2.33 [95% CI 1.21-4.47]) and a SOFA score >15 (HR 3.46 [95% CI 1.65-7.25). CONCLUSIONS AND RELEVANCE: Our analysis demonstrates the high prevalence of AKI requiring RRT among critically ill patients with COVID-19 and is associated with a high mortality, however, the rate of renal recovery is high among survivors and should inform shared-decision making.


Assuntos
Lesão Renal Aguda/etiologia , Lesão Renal Aguda/patologia , Rim/patologia , Lesão Renal Aguda/virologia , Idoso , Estado Terminal/mortalidade , Feminino , Humanos , Unidades de Terapia Intensiva , Rim/virologia , Masculino , Pessoa de Meia-Idade , Cidade de Nova Iorque , Modelos de Riscos Proporcionais , Terapia de Substituição Renal/métodos , Estudos Retrospectivos , Sobreviventes
3.
Med Sci Monit ; 26: e928788, 2020 Dec 18.
Artigo em Inglês | MEDLINE | ID: mdl-33338031

RESUMO

BACKGROUND Diabetic wound (DW) treatment is a serious challenge for clinicians, and the underlying mechanisms of DWs remain elusive. We sought to identify the critical genes in the development of DWs and provide potential targets for DW therapies. MATERIAL AND METHODS Datasets of GSE38396 from the Gene Expression Omnibus (GEO) database were reviewed. Pathway analysis was performed using the Kyoto Encyclopedia of Genes and Genomes (KEGG), Gene Ontology term analyses were carried out, and Cytoscape software (Cytoscape 3.7.2) was used to construct the protein interaction network. Serum samples from patients with diabetes and control participants were collected, and the expression of estrogen receptor 1 (ESR1) was measured by quantitative reverse-transcription polymerase chain reaction. In addition, the function of ESR1 in human skin fibroblasts was investigated in vitro. RESULTS Eight samples were analyzed using the Morpheus online tool, which identified 637 upregulated and 448 downregulated differentially expressed genes. The top 5 KEGG pathways of upregulated differentially expressed genes were associated with sphingolipid metabolism, estrogen signaling, ECM-receptor interaction, MAPK signaling, and PI3K-Akt signaling. The hub genes for DWs were JUN, ESR1, CD44, SMARCA4, MMP2, BMP4, GSK3B, WDR5, PTK2, and PTGS2. JUN, MMP2, and ESR1 were the upregulated hub genes, and ESR1 was found to be consistently enriched in DW patients. Inhibition of ESR1 had a stimulative role in human skin fibroblasts. CONCLUSIONS ESR1 was identified as a crucial gene in the development of DWs, which suggests potential therapeutic targets for DW healing.


Assuntos
Complicações do Diabetes/genética , Receptor alfa de Estrogênio/genética , Fibroblastos/metabolismo , Úlcera Cutânea , Células Cultivadas , Bases de Dados Genéticas , Regulação da Expressão Gênica , Ontologia Genética , Humanos , Transdução de Sinais , Úlcera Cutânea/etiologia , Úlcera Cutânea/genética , Úlcera Cutânea/metabolismo , Cicatrização/genética
4.
Cancer Imaging ; 20(1): 88, 2020 Dec 14.
Artigo em Inglês | MEDLINE | ID: mdl-33317609

RESUMO

BACKGROUND: Previous studies have indicated that quantitative MRI (qMR) is beneficial for diagnosis of breast cancer. As a novel qMR technology, synthetic MRI (syMRI) may be advantageous by offering simultaneous generation of T1 and T2 mapping in one scan within a few minutes and without concern to the deposition of the gadolinium contrast agent in cell nucleus. In this study, the potential of quantitative mapping derived from Synthetic MRI (SyMRI) to diagnose breast cancer was investigated. METHODS: From April 2018 to May 2019, a total of 87 patients with suspicious breast lesions underwent both conventional and SyMRI before treatment. The quantitative metrics derived from SyMRI, including T1 and T2 values, were measured in breast lesions. The diagnostic performance of SyMRI was evaluated with unpaired Student's t-tests, receiver operating characteristic curve analysis and multivariate logistic regression analysis. The AUCs of quantitative values were compared using Delong test. RESULTS: Among 77 patients who met the inclusion criteria, 48 were diagnosed with histopathological confirmed breast cancers, and the rest had benign lesions. The breast cancers showed significantly higher T1 (1611.61 ± 215.88 ms) values and lower T2 (80.93 ± 7.51 ms) values than benign lesions. The area under the ROC curve (AUC) values were 0.931 (95% CI: 0.874-0.989) and 0.883 (95% CI: 0.810-0.956) for T1 and T2 maps, respectively, in diagnostic discrimination between breast cancers and benign lesions. A slightly increased AUC of 0.978 (95% CI: 0.915-0.993) was achieved by combining those two relaxation-based quantitative metrics. CONCLUSION: In conclusion, our preliminary study showed that the quantitative T1 and T2 values obtained by SyMRI could distinguish effectively between benign and malignant breast lesions, and T1 relaxation time showed the highest diagnostic efficiency. Furthermore, combining the two quantitative relaxation metrics further improved their diagnostic performance.

5.
Aging (Albany NY) ; 12(21): 21290-21307, 2020 11 07.
Artigo em Inglês | MEDLINE | ID: mdl-33170152

RESUMO

BACKGROUND: Studies have demonstrated that S-adenosylmethionine could effectively affect the clinical wearing-off phenomena of levodopa, an antiparkinsonian agent; however, the detailed mechanisms for this effect need to be further clarified. RESULTS: S-adenosylmethionine and levodopa had opposite effects on the protein stability of vascular endothelial growth factor-A. The analysis of tube formation and cell viability also showed the nonconforming functions of S-adenosylmethionine and levodopa on cell angiogenesis and proliferation. Meanwhile, S-adenosylmethionine could significantly abolish the increased angiogenesis and cell viability induced by levodopa. S-adenosylmethionine resulted in G1/S phase arrest, with decreased cyclin dependent kinase 4/6 and increased p16, a specific cyclin dependent kinase inhibitor. Mechanically, the different effects of levodopa and S-adenosylmethionine were dependent on the phosphorylation and activation of extracellular signal-regulated kinase. S-adenosylmethionine could be fitted into the predicted docking pocket in the crystal structure of vascular endothelial growth factor-A, enhancing its acetylation level and reducing half-life. CONCLUSIONS: These observations suggested that methyl donor S-adenosylmethionine could act as a potential agent against vascular endothelial growth factor-A-related diseases induced by levodopa treatment. METHODS: We performed in vitro cytological analyses to assess whether S-adenosylmethionine intake could influence levodopa-induced vascular endothelial growth factor-A expression in human umbilical vein endothelial cells.

6.
Artigo em Inglês | MEDLINE | ID: mdl-33170780

RESUMO

Head-mounted loupes can increase the user's visual acuity to observe the details of an object. On the other hand, optical see-through head-mounted displays (OST-HMD) are able to provide virtual augmentations registered with real objects. In this paper, we propose AR-Loupe, combining the advantages of loupes and OST-HMDs, to offer augmented reality in the user's magnified field-of-vision. Specifically, AR-Loupe integrates a commercial OST-HMD, Magic Leap One, and binocular Galilean magnifying loupes, with customized 3D-printed attachments. We model the combination of user's eye, screen of OST-HMD, and the optical loupe as a pinhole camera. The calibration of AR-Loupe involves interactive view segmentation and an adapted version of stereo single point active alignment method (Stereo-SPAAM). We conducted a two-phase multi-user study to evaluate AR-Loupe. The users were able to achieve sub-millimeter accuracy (0.82mm) on average, which is significantly (p<0.001) smaller compared to normal AR guidance (1.49mm). The mean calibration time was 268.46s. With the increased size of real objects through optical magnification and the registered augmentation, AR-Loupe can aid users in high-precision tasks with better visual acuity and higher accuracy.

7.
Front Neurosci ; 14: 857, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33041748

RESUMO

Introduction: Cognitive impairment (CI) is a frequent non-motor symptom of Parkinson's disease (PD). Caudate and Apolipoprotein E (ApoE) are biomarkers linked to CI in PD. There is little known about whether ApoE affects caudate in mild CI of PD (PD-MCI). We investigated the possible interactive effect of ApoE genotypes on caudate functional connectivity (FC) in PD-MCI. Methods: A total of 95 PD-MCI patients and 99 matched healthy controls underwent extensive neuropsychological assessment and magnetic resonance imaging. The two groups were separated into three subgroups according to their genotyping. Functional data were analyzed with FC analysis. Results: Decreased FC between the caudate and the bilateral inferior orbit frontal gyrus and bilateral middle occipital gyrus (MOG) was found between groups, along with poor performance in general, executive, episodic memory, language, and visual-spatial function. Decreased FC between the caudate and right MOG, right middle temporal gyrus, and right superior occipital gyrus was found as an interaction effect. The FC values of ε4 carriers with PD-MCI were much lower than the other carriers, and FC was positively correlated with the impairment of global and language function. Conclusion: These results support the idea that altered FC between the bilateral caudate and posterior cortical regions was interactively influenced by ApoE genotype and PD-MCI status, and the ε4 subtype associated with underlying pathology of global cognitive decline and semantic fluency impairment in an interactive manner. Gene-based imaging approaches might strengthen the credibility in imaging genetic associations, which might provide new powerful insights into the neural mechanisms underlying PD-MCI.

8.
Front Pharmacol ; 11: 1083, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33041784

RESUMO

Our previous studies showed that Astragaloside IV derivative (LS-102) exhibited potent protective function against ischemia reperfusion (I/R) injury, but little is known about the mechanisms. Mitochondrial fission regulated by dynamin-related protein1 (Drp1) is a newly recognized determinant of mitochondrial function. This study aimed to investigate the protection of LS-102 on mitochondrial structure and function by regulating the activity of Drp1 using models of H9c2 cardiomyocyte injury induced by hypoxia-reperfusion (H/R), and rat heart injury induced by I/R. The results showed that LS-102 significantly decreased apoptosis, levels of ROS, CK, LDH, and calcium, upregulating MMP, and the Bax/Bcl-2 ratio in cardiomyocytes during I/R injury. Furthermore, LS-102 prevented I/R-induced mitochondrial fission by decreasing Drp1's mitochondrial localization through decreasing the phosphorylation of Drp1 at Ser616 (Drp1Ser616) and increasing the phosphorylation of Drp1 at Ser637 (Drp1Ser637) in H9c2 cells. Importantly, we also robustly confirmed Drp1Ser616 as a novel GSK-3ß phosphorylation site. GSK-3ß-mediated phosphorylation at Drp1Ser616 may be associated with mitochondrial fission during I/R of cardiomyocytes. In conclusion, LS-102 exerts cardio protection against I/R-induced injury by inhibiting mitochondrial fission via blocking GSK-3ß-mediated phosphorylation at Ser616 of Drp1.

9.
Abdom Radiol (NY) ; 2020 Oct 19.
Artigo em Inglês | MEDLINE | ID: mdl-33074425

RESUMO

OBJECTIVE: To explore the value of combined diffusion tensor imaging (DTI) and blood oxygenation level-dependent magnetic resonance imaging (BOLD-MRI) in detecting early renal alterations in patients with hyperuricemia. MATERIALS AND METHODS: Seventy-one individuals were enrolled in this study and divided into three groups according to their serum uric acid (SUA) level and clinical symptoms: healthy controls (HC, n = 23), asymptomatic hyperuricemia (AH, n = 22) and gouty arthritis (GA, n = 26). All patients underwent both DTI and BOLD-MRI examination. Renal cortical and medullary ADC, FA and R2* values were calculated, respectively, and compared among the three groups. Correlations between ADC, FA and R2* with estimated glomerular filtration rate (eGFR) and SUA in hyperuricemia were evaluated, respectively. RESULT: In the renal cortex, the ADC, FA and R2* values of the AH and GA groups were significantly lower than those of the HC groups (p < 0.05). In the renal medulla, the ADC and FA values in AH and GA patients were significantly lower than those in healthy controls (p < 0.05). The R2* value of the GA group significantly decreased, compared to that of the AH and HC groups (p < 0.05). SUA was negatively correlated with cortical ADC, FA and R2* values (p < 0.05) as well as with medullary ADC and FA values. No significant correlation was discovered between the eGFR and ADC, FA and R2* values. CONCLUSION: The combined evaluation of DTI and BOLD might provide a sensitive and non-invasive approach for detection of renal microstructural alterations and oxygen metabolism abnormality in hyperuricemia.

10.
Kidney Int Rep ; 5(8): 1149-1160, 2020 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-32775814

RESUMO

Introduction: There are limited data on the association of kidney dysfunction with prognosis in coronavirus disease 2019 (COVID-19), and the extent to which acute kidney injury (AKI) predisposes patients to severe illness and inferior outcomes is unclear. We aim to assess the incidence of AKI among patients with COVID-19 and examine their associations with patient outcomes as reported in the available literature thus far. Methods: We systematically searched MEDLINE, EMBASE, SCOPUS, and MedRxiv databases for full-text articles available in English published from December 1, 2019 to May 24, 2020. Clinical information was extracted and examined from 20 cohorts that met inclusion criteria, covering 13,137 mostly hospitalized patients confirmed to have COVID-19. Two authors independently extracted study characteristics, results, outcomes, study-level risk of bias, and strength of evidence across studies. Neither reviewer was blind to journal titles, study authors, or institutions. Results: Median age was 56 years, with 55% male patients. Approximately 43% of patients had severe COVID-19 infection, and approximately 11% died. Prevalence of AKI was 17%; 77% of patients with AKI experienced severe COVID-19 infection, and 52% died. AKI was associated with increased odds of death among COVID-19 patients (pooled odds ratio, 15.27; 95% CI 4.82-48.36), although there was considerable heterogeneity across studies and among different regions in the world. Approximately 5% of all patients required use of renal replacement therapy (RRT). Conclusions: Kidney dysfunction is common among patients with COVID-19, and patients who develop AKI have inferior outcomes. Additional research into management and potential mechanisms of this association is needed.

11.
Appl Res Qual Life ; : 1-18, 2020 Jun 29.
Artigo em Inglês | MEDLINE | ID: mdl-32837605

RESUMO

While quarantine has become a widely used control strategy during the outbreak of the 2019 novel coronavirus disease (COVID-19), empirical research on whether and to what extent quarantine and attitudes towards COVID-19 affect mental health is scant. Using a cross-sectional online survey, this paper is the first from the Chinese outbreak to investigate how quarantine experiences and attitudes towards COVID-19 are related to mental health, and how these relationships change across the distribution of mental health scores. Using quantile regression analysis, we found that home self-quarantine is associated with a decrease in depression and an increase in happiness, while community-level quarantine is associated with decreased happiness, especially for those in the lower happiness quantile. We also found that favorable attitudes towards COVID-19 regarding the credibility of real-time updates and confidence in the epidemic control are associated with lower levels of depression and higher levels of happiness. These effects are stronger in the upper quantile of depression and the median quantile of happiness.

12.
Nat Commun ; 11(1): 4226, 2020 08 24.
Artigo em Inglês | MEDLINE | ID: mdl-32839450

RESUMO

Intercellular signaling is indispensable for single cells to form complex biological structures, such as biofilms, tissues and organs. The genetic tools available for engineering intercellular signaling, however, are quite limited. Here we exploit the chemical diversity of biological small molecules to de novo design a genetic toolbox for high-performance, multi-channel cell-cell communications and biological computations. By biosynthetic pathway design for signal molecules, rational engineering of sensing promoters and directed evolution of sensing transcription factors, we obtain six cell-cell signaling channels in bacteria with orthogonality far exceeding the conventional quorum sensing systems and successfully transfer some of them into yeast and human cells. For demonstration, they are applied in cell consortia to generate bacterial colony-patterns using up to four signaling channels simultaneously and to implement distributed bio-computation containing seven different strains as basic units. This intercellular signaling toolbox paves the way for engineering complex multicellularity including artificial ecosystems and smart tissues.


Assuntos
Comunicação Celular/genética , Biologia Computacional/métodos , Transdução de Sinais/genética , Fatores de Transcrição/genética , Escherichia coli/genética , Escherichia coli/metabolismo , Proteínas de Fluorescência Verde/genética , Proteínas de Fluorescência Verde/metabolismo , Células HEK293 , Humanos , Microscopia de Fluorescência , Mutação , Reprodutibilidade dos Testes , Saccharomyces cerevisiae/genética , Saccharomyces cerevisiae/metabolismo , Fatores de Transcrição/metabolismo
13.
Neurol Sci ; 2020 Jul 11.
Artigo em Inglês | MEDLINE | ID: mdl-32654008

RESUMO

BACKGROUND: Pilots often face and need to overcome a diverse range of unfavorable conditions, of which hypoxic exposure is the most common. Studies have reported that hypoxia can induce a decrease in cerebral blood flow (CBF) in the brains of both humans and animals. Hypoxia and the associated cerebral hemodynamic changes can contribute to cognitive performance deficits that may endanger flight safety and increase the risk of accidents. AIM: In this study, we aimed to identify region-specific alterations in CBF in male pilots after exposure to hypoxia. MATERIAL AND METHODS: We used 3D pseudo-continuous arterial spin labeling sequences in 35 healthy male pilots (mean age: 30.6 ± 4.82 years) under simulated hypoxic conditions with a 3.0-T magnetic resonance imaging scanner. The generated CBF maps were measured and averaged in several regions of interest. RESULTS: Hypoxia decreased CBF in various brain regions, including the right temporal and bilateral occipital lobes, the anterior and posterior lobes of the cerebellum, the culmen and declive, and the inferior semilunar lobule of the cerebellum. CONCLUSION: These changes may impact the functional activity of the brains of pilots experiencing hypoxia in flight, but the related mechanisms require further investigation.

14.
Neurosci Lett ; 735: 135238, 2020 09 14.
Artigo em Inglês | MEDLINE | ID: mdl-32645398

RESUMO

OBJECTIVE: Depressive symptoms are the most common non-motor symptom accompanying Parkinson's disease (PD); however, the neural basis of depression in PD remains unclear. The aim of this study was to characterize possible depression-related white matter microstructural differences in patients with PD and comorbid depression compared with PD patients and healthy controls (HC) without depression. METHODS: We used diffusion tensor imaging (DTI) to analyze white matter integrity in depressed PD patients (n = 30), non-depressed PD patients (n = 43), and HC (n = 91). Five MRI-derived indices were estimated: fractional anisotropy (FA), mean diffusivity (MD), axial diffusivity (AD), radial diffusivity (RD), and white matter volume (WMV). RESULTS: Compared with HC and non-depressed PD, depressed PD patients showed significantly increased AD values in the body of corpus callosum, right anterior corona radiate, and left hippocampal part of the cingulum, as well as increased MD values in the left hippocampal part of the cingulum. CONCLUSIONS: Our results show that frontal and limbic white matter integrity is impaired in depressed PD patients. These findings can be used to better understand potential mechanisms of depression in PD.

15.
J Org Chem ; 85(15): 9514-9524, 2020 Aug 07.
Artigo em Inglês | MEDLINE | ID: mdl-32515197

RESUMO

The ruthenium-catalyzed activation of the C4 position of coumarins for coupling with acrylates was described using modifiable ketone as a directing group. The alkenylation reaction provided a direct approach to prepare previously inaccessible 4-alkenylated coumarins with operational simplicity and high atom-economy. This protocol also worked well with coumarin-3-carboxylic acids to unveil a rare instance of a tandem alkenylation/decarboxylation reaction. The potential value of this approach was further highlighted by the efficient synthesis of several heterocyclic fused coumarin derivatives.

16.
Brain Res Bull ; 161: 33-42, 2020 08.
Artigo em Inglês | MEDLINE | ID: mdl-32387084

RESUMO

Whether GPR17 has the same distribution and repair mechanism in immature white matter with periventricular leukomalacia (PVL) as in the adult brain remains to be determined. This study tried to explore the expression phase and site of GPR17, and to investigate the effect of silencing GPR17 on endogenous repair mechanism of immature white matter with PVL. Ischemic PVL in vivo results showed that GPR17 gene and protein expression increased more in the PVL than in the sham group at 12 h-24 h and 72h to 7 days after PVL. NG2+/GPR17+progenitor cells at 48 h-96 h and O4+/GPR17+precursor cells at 72h to 7d were also significantly increased in the PVL compared to the sham groups. Results in vitro showed that oxygen-glucose deprivation (OGD) also induced more GPR17 gene and protein expression than control at 48 h-72 h. There were more NG2+/GPR17+progenitor cells at 24 h-48 h and O4+/GPR17+precursor cells at 48 h-72 h in the OGD groups, as well. The functional role of GPR17 in the intrinsic repair response to ischemia was tested using GPR17 gene silencing. The progenitor cells and OL precursors in the OGD+GPR17 silencing group were both significantly less than those in the control, OGD and OGD+gene silencing control groups. The apoptotic percentage of cells in OGD+GPR17 silencing group was also much higher. In summary, ischemia-induced GPR17 expression was shown to contribute to glial-derived progenitor cell proliferation and differentiation into OL precursors, which may provide a therapeutic target for immature neonatal white matter injury after ischemia.

17.
Oxid Med Cell Longev ; 2020: 2198976, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32411321

RESUMO

Endocytosis is a protective mechanism of renal epithelial cells to eliminate retained crystals. This research investigated the endocytosis of 100 nm calcium oxalate monohydrate crystals in human kidney proximal tubular epithelial (HK-2) cells before and after repair by four kinds of tea polysaccharides with molecular weights (MWs) of 10.88 (TPS0), 8.16 (TPS1), 4.82 (TPS2), and 2.31 kDa (TPS3), respectively. When HK-2 cells were repaired by TPSs after oxalic acid injury, the cell viability, wound healing ability, mitochondrial membrane potential, percentage of cells with endocytosed crystals, and dissolution rate of the endocytosed crystals increased; the cell morphology recovered; and the reactive oxygen level and lactate dehydrogenase release decreased. Most of the endocytosed crystals were found in the lysosomes. The repair effects of the four TPSs were ranked in the following order: TPS2>TPS1>TPS3>TPS0. TPS2 with moderate MW presented the optimal repair ability and strongest ability to promote endocytosis.


Assuntos
Oxalato de Cálcio/metabolismo , Endocitose , Nanopartículas/química , Polissacarídeos/farmacologia , Chá/química , Linhagem Celular , Forma Celular/efeitos dos fármacos , Endocitose/efeitos dos fármacos , Fluorescência , Humanos , L-Lactato Desidrogenase/metabolismo , Lisossomos/efeitos dos fármacos , Lisossomos/metabolismo , Potencial da Membrana Mitocondrial/efeitos dos fármacos , Espécies Reativas de Oxigênio/metabolismo , Cicatrização/efeitos dos fármacos
18.
J Clin Rheumatol ; 26(4): 134-141, 2020 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-32453286

RESUMO

OBJECTIVES: The aim of this study is to investigate whether heat shock protein 70 (Hsp70) gene polymorphisms are implicated in systemic lupus erythematous (SLE) susceptibility, the efficacy of glucocorticoids (GCs) treatment, and improvement of health-related quality of life. METHODS: A total of 499 SLE patients and 499 controls were included in a case-control study, and 468 SLE patients treated with GCs for 12 weeks were involved in a follow-up study. Patients who completed the 12-week follow-up were divided into GCs-sensitive and GCs-insensitive group by using the SLE disease activity index. The SF-36 was used to evaluate the health-related quality of life of SLE patients, and genotyping was performed by improved multiplex ligation detection reaction. RESULTS: rs2075800 was associated with SLE susceptibility (adjusted odds ratio [ORadj], 1.437; 95% confidence interval [CI], 1.113-1.855; Padj = 0.005; PBH = 0.020 by dominant model; ORadj, 1.602; 95% CI, 1.072-2.395; Padj = 0.022; PBH = 0.029 by TT vs CC model; ORadj = 1.396; 95% CI = 1.067-1.826; Padj = 0.015; PBH = 0.029 by TC vs CC model). In the follow-up study, rs2075799 was associated with the improvement in mental health (p = 0.004, PBH = 0.044), but we failed to find any association between the efficacy of GCs and Hsp70 gene polymorphisms. CONCLUSIONS: Hsp70 gene polymorphisms may be associated with susceptibility to SLE and improvement of mental health in Chinese Han population.

19.
Artigo em Inglês | MEDLINE | ID: mdl-32337747

RESUMO

Cyanobacterial exopolysaccharides (EPS) accumulated during microalgal cultivation have significant application potential in antioxidation, pharmaceutical products, and so on. Inoculation concentration strongly affects the cultivation cost, biomass, and EPS accumulation. In this study, a high-EPS-excreted desert cyanobacterium Microcoleus vaginatus was isolated, and the effects of inoculation concentration on biomass, photosynthetic activity, and EPS accumulation were explored. The results showed that the original fluorescence (Fo ) provided a good indication to cyanobacterial biomass, when Chl-a concentration was lower than 10 mg L-1 . Inoculation concentration significantly affected cyanobacterial biomass and EPS concentration (P < 0.001), whereas did not affect photosynthetic activity (Fv /Fm ; P > 0.05). The two fractions of EPS, capsular exopolysaccharides (CPS) and released exopolysaccharides (RPS) were strongly affect by inoculation concentration. Other than forming thick sheath (CPS) surrounded the filaments, M. vaginatus excreted higher proportions of RPS to culture medium, and the ratio of RPS to CPS ranged from 1.08 to 1.58 depending on the inoculation concentration. Additionally, although the biomass and EPS accumulation increased with inoculation concentration, the increasing inoculation concentration did not bring to the proportionate increase of the final biomass and EPS yield. Altogether, comprehensively considering the EPS yield and productivity, inoculation concentration of 0.04 mg Chl-a L-1 is recommended for M. vaginatus to produce EPS, with an EPS yield of 94.32 mg L-1 and EPS productivity of 184.86 mg (mg Chl-a)-1  L-1  d-1 at the end of experiment.

20.
Biochem Biophys Res Commun ; 526(3): 713-720, 2020 06 04.
Artigo em Inglês | MEDLINE | ID: mdl-32253030

RESUMO

Gastric cancer (GC) remains a serious threat to human health with a high cancer-related death rate and unsatisfactory treatment effects after curative resection, especially with advanced GC. Thus, exploration of the molecular mechanism of cisplatin (CDDP) resistance in GC is crucial. circCCDC66 (hsa_circ_0001313) expression was detected by quantitative reverse-transcription PCR in GC cell lines and tissues. The characteristics of circCCDC66 in CDDP resistance in GC were evaluated in vivo and vitro. We performed luciferin reporter assays, biotin-coupled RNA pull-downs and fluorescence in situ hybridization (FISH) to assess the relationship of miR-618 to circCCDC66. Function was determined by cytotoxicity assay, western immunoblotting and TUNEL. CircCCDC66 was overexpressed in CDDP-resistant cells and tissues. The circCCDC66 expression was significantly associated with malignancy and was an independent risk factor for disease-free survival (DFS) in GC patients treated by CDDP based chemotherapy. Data from in vitro and vivo experiments demonstrated that circCCDC66 inhibited apoptosis by targeting miR-618 and release of B-cell lymphoma-2 (BCL2). CircCCDC66 is an essential regulator in the development of CDDP resistance and may serve as a promising therapeutic target for GC patients. Otherwise, our study adds more evidence of circRNA functioning as a sequestering agent for miRNA.


Assuntos
Cisplatino/farmacologia , Resistencia a Medicamentos Antineoplásicos/genética , MicroRNAs/metabolismo , Proteínas Proto-Oncogênicas c-bcl-2/metabolismo , RNA Circular/metabolismo , Neoplasias Gástricas/tratamento farmacológico , Animais , Apoptose/efeitos dos fármacos , Linhagem Celular Tumoral , Intervalo Livre de Doença , Feminino , Regulação Neoplásica da Expressão Gênica , Humanos , Hibridização in Situ Fluorescente , Infecções/metabolismo , Lentivirus/genética , Camundongos Endogâmicos BALB C , Camundongos Nus , Transdução de Sinais
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