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1.
Dev Sci ; : e13191, 2021 Nov 14.
Artigo em Inglês | MEDLINE | ID: mdl-34775669

RESUMO

The goal of the present research was to assess whether children's first interaction with a single outgroup member can significantly impact their general attitudes toward the outgroup as a whole. In two preregistered studies, 5- to 6-year-old Chinese children (total N = 147) encountered a Black adult from another country for the very first time, and they played a game together. General attitudes toward the outgroup were assessed using both implicit and explicit measures. In both studies, the interaction resulted in less negative explicit attitudes toward Black people, but more negative implicit attitudes. The results demonstrate for the first time that one encounter with a single outgroup member can impact children's general attitudes toward that group, and that it can have differential effects on implicit and explicit attitudes.

2.
Zhongguo Dang Dai Er Ke Za Zhi ; 23(6): 593-598, 2021 Jun.
Artigo em Chinês | MEDLINE | ID: mdl-34130781

RESUMO

OBJECTIVE: To evaluate the clinical features of preterm infants with a birth weight less than 1 500 g undergoing different intensities of resuscitation. METHODS: A retrospective analysis was performed for the preterm infants with a birth weight less than 1 500 g and a gestational age less than 32 weeks who were treated in the neonatal intensive care unit of 20 hospitals in Jiangsu, China from January 2018 to December 2019. According to the intensity of resuscitation in the delivery room, the infants were divided into three groups:non-tracheal intubation (n=1 184), tracheal intubation (n=166), and extensive cardiopulmonary resuscitation (ECPR; n=116). The three groups were compared in terms of general information and clinical outcomes. RESULTS: Compared with the non-tracheal intubation group, the tracheal intubation and ECPR groups had significantly lower rates of cesarean section and use of antenatal corticosteroid (P < 0.05). As the intensity of resuscitation increased, the Apgar scores at 1 minute and 5 minutes gradually decreased (P < 0.05), and the proportion of infants with Apgar scores of 0 to 3 at 1 minute and 5 minutes gradually increased (P < 0.05). Compared with the non-tracheal intubation group, the tracheal intubation and ECPR groups had significantly higher mortality rate and incidence rates of moderate-severe bronchopulmonary dysplasia and serious complications (P < 0.05). The incidence rates of grade Ⅲ-Ⅳ intracranial hemorrhage and retinopathy of prematurity (stage Ⅲ or above) in the tracheal intubation group were significantly higher than those in the non-tracheal intubation group (P < 0.05). CONCLUSIONS: For preterm infants with a birth weight less than 1 500 g, the higher intensity of resuscitation in the delivery room is related to lower rate of antenatal corticosteroid therapy, lower gestational age, and lower birth weight. The infants undergoing tracheal intubation or ECRP in the delivery room have an increased incidence rate of adverse clinical outcomes. This suggests that it is important to improve the quality of perinatal management and delivery room resuscitation to improve the prognosis of the infants.


Assuntos
Cesárea , Recém-Nascido Prematuro , Peso ao Nascer , China , Feminino , Idade Gestacional , Humanos , Lactente , Recém-Nascido , Gravidez , Estudos Retrospectivos
3.
Contemp Clin Trials ; 103: 106337, 2021 04.
Artigo em Inglês | MEDLINE | ID: mdl-33662589

RESUMO

Preterm infants constitute an important proportion of neonatal deaths and various complications, and very preterm infants (VPI) are more likely to develop severe complications, such as intraventricular hemorrhage (IVH), anemia, and sepsis. It has been confirmed that placental transfusion can supplement blood volume in infants and reduce preterm-associated complications, which is further conducive to the development of the nervous system and a better long-term prognosis. Based on these advantages, placental transfusion has been widely used in VPI. There are three main types of placental transfusion: delayed cord clamping (DCC), intact umbilical cord milking (I-UCM), and cut umbilical cord milking (C-UCM). However, the optimal method for PT-VPI remains controversial, and it is urgent to identify the best method of placental transfusion. We plan to fully evaluate the safety and effectiveness of these three placental transfusion methods in VPI in a 3-arm multicenter randomized controlled trial: Placental Transfusion in Very Preterm Infants (PT-VPI). Trial registration: chictr.org.cn, number ChiCTR2000030953.


Assuntos
Recém-Nascido Prematuro , Placenta , Transfusão de Sangue , Constrição , Feminino , Humanos , Recém-Nascido , Gravidez , Cordão Umbilical
4.
Dev Psychol ; 57(3): 386-396, 2021 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-33570986

RESUMO

Age-related differences in explicit and implicit racial biases in Black Cameroonians (N = 187, 94 females) were investigated using a cross-sectional design. Participants ranged in age from 3 to 30 years, and were from middle-to-high income families in Yaoundé, Cameroon. Biases were assessed by comparing attitudes toward Blacks with those toward Whites and Chinese. Implicit pro-Black/anti-other-race (White and Chinese) biases were present at age 4, in contrast to anti-Black/pro-other-race biases, which were observed among 9- to 30-year-olds. In addition, explicit pro-Black/anti-other-race biases that were present at age 4 were no longer evident by age 7. These findings provide a detailed picture of age-related differences in racial biases in an understudied part of the world that can inform theories regarding the development of racial biases, as well as efforts to reduce such biases. (PsycInfo Database Record (c) 2021 APA, all rights reserved).


Assuntos
Fatores Etários , Racismo , Adolescente , Adulto , Afro-Americanos , Viés , Camarões , Criança , Pré-Escolar , Estudos Transversais , Grupo com Ancestrais do Continente Europeu , Feminino , Humanos , Masculino , Adulto Jovem
5.
Int J Antimicrob Agents ; 57(3): 106300, 2021 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-33567334

RESUMO

INTRODUCTION: Few studies incorporating population pharmacokinetic/pharmacodynamic (Pop-PK/PD) modelling have been conducted to quantify the exposure target of vancomycin in neonates. A retrospective observational cohort study was undertaken in neonates to determine this target and dosing recommendations (chictr.org.cn, ChiCTR1900027919). METHODS: A Pop-PK model was developed to estimate PK parameters. Causalities between acute kidney injury (AKI) occurrence and vancomycin use were verified using Naranjo criteria. Thresholds of vancomycin exposure in predicting AKI or efficacy were identified via classification and regression tree analysis. Associations between exposure thresholds and clinical outcomes, including AKI and efficacy, were analysed by logistic regression. Dosing recommendations were designed using Monte Carlo simulations based on the optimised exposure target. RESULTS: Pop-PK modelling included 182 neonates with 411 observations. On covariate analysis, neonatal physiological maturation, renal function and concomitant use of vasoactive agents (VAS) significantly affected vancomycin PK. Seven cases of vancomycin-induced AKI were detected. Area under the concentration-time curve from 0-24 hours (AUC0-24) ≥ 485 mg•h/L was an independent risk factor for AKI after adjusting for VAS co-administration. The clinical efficacy of vancomycin was analysed in 42 patients with blood culture-proven staphylococcal sepsis. AUC0-24 to minimum inhibitory concentration (AUC0-24/MIC) ≥ 234 was the only significant predictor of clinical effectiveness. Monte Carlo simulations indicated that regimens in Neonatal Formulary 7 and Red Book (2018) were unsuitable for all neonates. CONCLUSION: An AUC0-24 of 240-480 (assuming MIC = 1 mg/L) is a recommended exposure target of vancomycin in neonates. Model-informed dosing regimens are valuable in clinical practice.


Assuntos
Injúria Renal Aguda/induzido quimicamente , Antibacterianos/administração & dosagem , Vancomicina/administração & dosagem , Vancomicina/farmacocinética , Antibacterianos/farmacocinética , Área Sob a Curva , Relação Dose-Resposta a Droga , Cálculos da Dosagem de Medicamento , Monitoramento de Medicamentos , Feminino , Humanos , Recém-Nascido , Masculino , Testes de Sensibilidade Microbiana , Método de Monte Carlo , Estudos Retrospectivos , Infecções Estafilocócicas/tratamento farmacológico , Staphylococcus/efeitos dos fármacos , Staphylococcus/isolamento & purificação , Resultado do Tratamento
7.
Arch Sex Behav ; 50(3): 807-820, 2021 04.
Artigo em Inglês | MEDLINE | ID: mdl-33169294

RESUMO

From a young age, children's peer appraisals are influenced by the social categories to which peers belong based on factors such as race and gender. To date, research regarding the manner in which race- and gender-related factors might interact to influence these appraisals has been limited. The present study employed an experimental vignette paradigm to investigate the relative influences of target peers' race, gender, and gender-typed behavior toward 4- to 6-year-old Chinese children's (N = 119, 62 girls, 57 boys) peer appraisals. Appraisals were assessed via (1) a rating scale measuring children's interest in being friends with a range of hypothetical target peers varying in race, gender, and gender-typed behavior, and (2) a forced-choice rank-order task in which children indicated their preferences for four hypothetical target peers who varied from themselves on either race, gender, or gender-typed behavior, or were similar to themselves on all three traits. There was little evidence to suggest children's rank-ordered peer preferences in relation to race were influenced by whether the other-race presented was White (preferred relatively more) or Black (preferred relatively less). In contrast, gender-related factors (i.e., rater gender, target gender, target gender-typed behavior) had more robust influences on peer preferences for both outcome measures. Gender-conforming peers were preferred over gender-nonconforming peers, and target boys displaying feminine behavior were less preferred than target girls displaying masculine behavior. The results help characterize cross-cultural (in)consistencies in children's social preferences in relation to peers' race and gender.


Assuntos
Grupos de Populações Continentais/psicologia , Amigos/psicologia , Comportamento Social , Criança , Pré-Escolar , Feminino , Identidade de Gênero , Humanos , Masculino
8.
Huan Jing Ke Xue ; 41(9): 4161-4168, 2020 Sep 08.
Artigo em Chinês | MEDLINE | ID: mdl-33124298

RESUMO

A single-stage PN-ANAMMOX (PN/A) granular sludge cultured at room temperature was used to investigate the completely autotrophic nitrogen removal efficiency and microbial community structure of low-strength wastewater based on the completely autotrophic nitrogen removal over nitrite in granular sludge at a low temperature. The results showed that at the low temperature (15±1)℃, the ammonia nitrogen load was maintained at 1.29 kg ·(m3 ·d)-1, and the ammonia nitrogen concentration in the injection was gradually reduced from 70 mg ·L-1 to 40 mg ·L-1. DO/TAN was controlled at 0.22-0.25. The total nitrogen removal rate was maintained at (85±4)%, and the average TN concentration in the effluent was 8.9 mg ·L-1. There was no significant proliferation of nitrite-oxidizing bacteria (NOB) during the operation period, and the Nitrospira abundance was less than 1%. Elutriation of the floc sludge and the control of low DO/TAN values can be used as effective control strategies to inhibit NOB proliferation. Through completely autotrophic nitrogen removal over nitrite in granular sludge operated under low-temperature and low-substrate conditions, the particle size became smaller, and the color changed from brown red to brown yellow. The total amount of PS decreased slightly, and the ratio of PN/PS stabilized at 2.5-3.0. Planctomycetes and Proteobacteria dominated the community, and Candidatus_Kuenenia and Candidatus_Brocadia were two AMX bacteria in the sludge.


Assuntos
Nitrogênio , Esgotos , Reatores Biológicos , Desnitrificação , Oxirredução , Temperatura
9.
J Exp Child Psychol ; 198: 104884, 2020 10.
Artigo em Inglês | MEDLINE | ID: mdl-32645522

RESUMO

The early emergence of racial biases points to the urgent need to understand how interpersonal experiences might shape them. We examined whether interpersonal movement shapes racial biases among 4- to 6-year-old Chinese children who had no prior contact with Black people. In Experiment 1 (N = 134), children played a musical game, moving either in or out of synchrony with a Chinese or Black adult. In Experiment 2 (N = 30), children were merely exposed to a Black adult. Across the two experiments, we found that synchronous movement increased children's feeling of social closeness toward their movement partner to a greater extent than asynchronous movement regardless of the partner's race. After moving in or out of synchrony with the Chinese adult, synchrony selectively increased children's explicit positive pro-own-race bias. However, after moving in or out of synchrony with the Black adult, both movement styles reduced explicit anti-other-race bias. Experiment 2 ruled out mere exposure to an other-race person as a driving factor for these effects. Our results suggest that musical engagement may be a promising intervention for reducing negative intergroup biases.


Assuntos
Desenvolvimento Infantil , Música , Racismo , Interação Social , Percepção Social , Criança , Pré-Escolar , Feminino , Humanos , Masculino , Fatores de Tempo
10.
Jpn J Infect Dis ; 73(3): 226-230, 2020 May 22.
Artigo em Inglês | MEDLINE | ID: mdl-32009054

RESUMO

Varicella-zoster virus (VZV) is a ubiquitous human herpesvirus that causes chickenpox and zoster. Considering that VZV is a relatively and genetically stable virus, its global surveillance clades provide essential information for VZV evolution, immigration, and importation of different viral strains and recombination events. Eighty-eight VZV isolates from China (Shanghai and Urumqi) were genotyped using a scattered single-nucleotide polymorphism method in this prospective study. Our results were based on sequencing the open reading frames 1, 6, 12, 16, 17, 21, 22, 35, 37, 38, 50, 54, 55, 56, 60, and 66. We found that the majority of these 88 strains (81.8%) belonged to Clade 2 with significantly high homogeneity from Shanghai. However, in the Urumqi area, some strains were grouped to Clade 5, and some could not be attributed to any of the established VZV clades, although the majority of Urumqi strains belonged to Clade 2. Our results illustrated that due to geographical location, VZV could undergo genetic recombination, suggesting that VZV diversity is more complicated in certain areas and geographical separation contributes to VZV complexity.


Assuntos
Genótipo , Herpes Zoster/virologia , Herpesvirus Humano 3/genética , Polimorfismo de Nucleotídeo Único , China/epidemiologia , Geografia , Herpes Zoster/epidemiologia , Humanos , Fases de Leitura Aberta , Estudos Prospectivos , Análise de Sequência de DNA
11.
Dev Psychol ; 56(5): 888-896, 2020 May.
Artigo em Inglês | MEDLINE | ID: mdl-31999181

RESUMO

Bilingual children have been shown to differ from monolingual children in several domains of human cognition. Comparatively few studies have investigated social-interactional processes in bilingual populations. Here, we investigated whether monolingual and bilingual children demonstrate similar susceptibility to an aspect of social functioning with broad societal reach: racial bias. We measured both implicit and explicit biases against African race individuals in 2 groups of monolingual preschoolers (native speakers of English or Chinese) and in 2 groups of English-Chinese bilingual preschoolers (tested in English or Chinese; total N = 160). We found that monolingual children demonstrated greater implicit bias against African race individuals than bilingual children, independent of their native language. Monolingual Chinese children demonstrated greater explicit bias than bilingual children, although monolingual English children's explicit bias scores did not differ from those of bilingual children. Findings are discussed in terms of cognitive and experiential mechanisms that may link bilingualism and racial bias. (PsycInfo Database Record (c) 2020 APA, all rights reserved).


Assuntos
Cognição , Grupos Étnicos , Multilinguismo , Racismo , Pré-Escolar , Feminino , Humanos , Relações Interpessoais , Masculino
12.
J Vis Exp ; (150)2019 08 19.
Artigo em Inglês | MEDLINE | ID: mdl-31475966

RESUMO

Impinging dryness is now a widely used and effective way for fabric drying due to its high heat and mass transfer coefficient. Previous studies on fabric drying have neglected the contributions of moisture uniformity and diffusion coefficient to the drying process; though, they have recently been shown to have a significant influence on drying characteristics. This report outlines a step-by-step procedure to investigate the effects of air impingement parameters on a fabric's drying characteristics by controlling the uniformity of its area moisture distribution. A hot air blower unit equipped with an angle adjustable nozzle is used to generate air flow with different velocities and temperatures while the drying process is recorded and analyzed using an infrared thermograph. In addition, a uniform padder is adapted to ensure the fabric's moisture uniformity. Impinging drying is studied under different initial conditions by changing the air flow temperature, velocity, and direction, then the applicability and suitability of the protocol are evaluated.


Assuntos
Têxteis , Ar , Fibra de Algodão , Temperatura , Água
13.
Dev Psychol ; 55(7): 1440-1452, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-30945884

RESUMO

We investigated the developmental courses of both implicit and explicit racial biases in relation to the perceived social status of outgroups. We did so by assessing these biases among Chinese participants (N = 200, age range from 4- to 19-year-olds) toward 2 different other-race groups that differ in terms of perceived social status (i.e., Whites and Blacks). At the youngest age, children showed both implicit anti-White and anti-Black bias at similar levels. However, these biases had different patterns of age-related change: implicit anti-Black bias remained strong and stable over time, whereas implicit anti-White bias declined after age 10. For explicit bias, children showed a decline in anti-Black and anti-White bias. Implicit and explicit biases were uncorrelated at all ages. The observed age-related changes demonstrate that it is possible for patterns of biases toward different races to diverge with age, and that perceived social status may contribute to the differential developmental patterns. (PsycINFO Database Record (c) 2019 APA, all rights reserved).


Assuntos
Desenvolvimento Infantil , Grupos Étnicos/psicologia , Racismo/psicologia , Adolescente , Adulto , Fatores Etários , Grupo com Ancestrais do Continente Asiático , Criança , Pré-Escolar , Feminino , Humanos , Masculino , Fatores Socioeconômicos , Adulto Jovem
14.
Child Dev ; 90(1): 162-179, 2019 01.
Artigo em Inglês | MEDLINE | ID: mdl-28605007

RESUMO

This research investigated the relation between racial categorization and implicit racial bias in majority and minority children. Chinese and Indian 3- to 7-year-olds from Singapore (N = 158) categorized Chinese and Indian faces by race and had their implicit and explicit racial biases measured. Majority Chinese children, but not minority Indian children, showed implicit bias favoring own race. Regardless of ethnicity, children's racial categorization performance correlated positively with implicit racial bias. Also, Chinese children, but not Indian children, displayed explicit bias favoring own race. Furthermore, children's explicit bias was unrelated to racial categorization performance and implicit bias. The findings support a perceptual-social linkage in the emergence of implicit racial bias and have implications for designing programs to promote interracial harmony.


Assuntos
Grupos Étnicos , Grupos Minoritários , Racismo/etnologia , Percepção Social , Criança , Pré-Escolar , China/etnologia , Feminino , Humanos , Índia/etnologia , Masculino , Singapura/etnologia
15.
Child Dev ; 90(3): e290-e305, 2019 05.
Artigo em Inglês | MEDLINE | ID: mdl-29023649

RESUMO

This study tracked the long-term effect of perceptual individuation training on reducing 5-year-old Chinese children's (N = 95, Mage  = 5.64 years) implicit pro-Asian/anti-Black racial bias. Initial training to individuate other-race Black faces, followed by supplementary training occurring 1 week later, resulted in a long-term reduction of pro-Asian/anti-Black bias (70 days). In contrast, training Chinese children to recognize White or Asian faces had no effect on pro-Asian/anti-Black bias. Theoretically, the finding that individuation training can have a long-term effect on reducing implicit racial bias in preschoolers suggests that a developmentally early causal linkage between perceptual and social processing of faces is not a transitory phenomenon. Practically, the data point to an effective intervention method for reducing implicit racism in young children.


Assuntos
Individuação , Racismo/etnologia , Afro-Americanos , Grupo com Ancestrais do Continente Asiático , Pré-Escolar , China/etnologia , Discriminação Psicológica/fisiologia , Grupo com Ancestrais do Continente Europeu , Reconhecimento Facial , Feminino , Humanos , Masculino , Satisfação Pessoal , Racismo/psicologia , Percepção Social
16.
Cancer Cell Int ; 18: 200, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-30534001

RESUMO

Background: Apoptosis and autophagy are known to play important roles in cancer development. It has been reported that HVJ-E induces apoptosis in cancer cells, thereby inhibiting the development of tumors. To define the mechanism by which HVJ-E induces cell death, we examined whether HVJ-E activates autophagic and apoptotic signaling pathways in HeLa cells. Methods: Cells were treated with chloroquine (CQ) and rapamycin to determine whether autophagy is involved in HVJ-E-induced apoptosis. Treatment with the ERK inhibitor, U0126, was used to determine whether autophagy and apoptosis are mediated by the ERK pathway. Activators of the PI3K/Akt/mTOR/p70S6K pathway, 740 Y-P and SC79, were used to characterize its role in HVJ-E-induced autophagy. siRNA against Atg3 was used to knock down the protein and determine whether it plays a role in HVJ-E-induced apoptosis in HeLa cells. Results: We found that HVJ-E infection inhibited cell viability and induced apoptosis through the mitochondrial pathway, as evidenced by the expression of caspase proteins. This process was promoted by rapamycin treatment and inhibited by CQ treatment. HVJ-E-induced autophagy was further blocked by 740 Y-P, SC79, and U0126, indicating that both the ERK- and the PI3K/Akt/mTOR/p70S6K-pathways were involved. Finally, autophagy-mediated apoptosis induced by HVJ-E was inhibited by siRNA-mediated Atg3 knockdown. Conclusion: In HeLa cells, HVJ-E infection triggered autophagy through the PI3K/Akt/mTOR/p70S6K pathway in an ERK1/2-dependent manner, and the induction of autophagy promoted apoptosis in an Atg3-dependent manner.

17.
Med Sci Monit ; 24: 8583-8591, 2018 Nov 27.
Artigo em Inglês | MEDLINE | ID: mdl-30481165

RESUMO

BACKGROUND UV-related skin disease such as actinic keratosis is a major concern in public health. In view of the cell injury induced by UVB, Klotho protein it is an ideal therapy to eliminate UVB-induced cell damages and the associated signaling pathways. MATERIAL AND METHODS To gain insights into the potential role of Klotho and the underlying molecular mechanism, we constructed a Klotho-overexpress HaCaT cell line and assessed the protection against UVB insults. The effects of exposure to UVB radiation on the human keratinocyte HaCaT cells, including cell growth, apoptosis, and changes of selected biomarkers, were measured by CCK-8, flow cytometry, Quantitative real-time PCR, and Western blot analysis. RESULTS We found that enhanced NF-κB activity was accompanied by decreased expression of the anti-aging protein Klotho upon UVB stimulation, which was further confirmed with in vivo experiments. Overexpression of Klotho was able to considerably alleviate the UVB-induced damages to cells and reversed the UVB-caused biomarker changes to a great extent, which was comparable to the effects of administration of NF-κB inhibitor PDTC, suggesting the inhibition of nuclear translocation and DNA-binding activity of NF-κB. Furthermore, Klotho overexpression was proved to decrease the nuclear expression of NF-κB as much as the treatment with PDTC, which provides support for the direct regulation of NF-κB by Klotho. CONCLUSIONS Collectively, our work provides new insight into the potential role of Klotho in the context of UVB-induced injuries in human keratinocytes, as well as providing the basis for future study of new therapies against UV-related skin disease.


Assuntos
Glucuronidase/metabolismo , Queratinócitos/metabolismo , Apoptose/efeitos dos fármacos , Ciclo Celular/efeitos dos fármacos , Linhagem Celular , Proliferação de Células/efeitos dos fármacos , Humanos , Queratinócitos/fisiologia , NF-kappa B/metabolismo , NF-kappa B/efeitos da radiação , Substâncias Protetoras/farmacologia , Protetores contra Radiação/farmacologia , Transdução de Sinais/efeitos dos fármacos , Raios Ultravioleta/efeitos adversos
18.
Biomed Environ Sci ; 31(4): 280-289, 2018 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-29773091

RESUMO

OBJECTIVE: The current study aims to investigate the effect of Hemagglutinating virus of Japan envelope (HVJ-E) on induction of apoptosis and autophagy in human prostate cancer PC3 cells, and the underlying mechanisms. METHODS: PC3 cells were treated with HVJ-E at various multiplicity of infection (MOI), and the generated reactive oxygen species (ROS), cell viability, apoptosis, and autophagy were detected, respectively. Next, the role of ROS played in the regulation of HVJ-E-induced apoptosis and autuphagy in PC3 cells were analysed. In the end, the relationship between HVJ-E-induced apoptosis and autuophagy was investigated by using rapamycin and chloroquine. RESULTS: Flow cytometry assay revealed that HVJ-E treatment induced dose-dependent apoptosis and that the JNK and p38 MAPK signaling pathways were involved in HVJ-E-induced apoptosis in PC3 cells. In addition, HVJ-E was able to induce autophagy in PC3 cells via the class III PI3K/beclin-1 pathway. The data also implyed that HVJ-E-triggered autophagy and apoptosis were ROS dependent. When ROS was blocked with N-acetylcysteine (NAC), HVJ-E-induced LC3-II conversion and apoptosis were reversed. Interestingly, HVJ-E-induced apoptosis was significantly increased by an inducer of autophagy, rapamycin pretreatment, both in vitro and in vivo. CONCLUSION: HVJ-E exerts anticancer effects via autophagic cell death in prostate cancer cells.


Assuntos
Apoptose/fisiologia , Autofagia/fisiologia , Neoplasias da Próstata/metabolismo , Espécies Reativas de Oxigênio/metabolismo , Vírus Sendai/imunologia , Inativação de Vírus , Linhagem Celular Tumoral , Sobrevivência Celular , Humanos , Masculino , Terapia Viral Oncolítica , Vírus Sendai/fisiologia
19.
Gene ; 651: 161-165, 2018 Apr 20.
Artigo em Inglês | MEDLINE | ID: mdl-29409738

RESUMO

BACKGROUND: Genome-wide association study (GWAS) studies have showed that single nucleotide polymorphisms (SNPs) in OCA2 gene were associated with the survival of breast cancer patients treated with adjuvant chemotherapy. To further explain the association between OCA2 SNPs and breast cancer survival, we investigated the predictive value of rs4778137 located in OCA2 in local advanced breast cancer patients receiving neoadjuvant chemotherapy. PATIENTS AND METHODS: A case-cohort with 150 breast cancer patients was performed to evaluate the effects of the OCA2 rs4778137 on breast cancer survival. The association between rs4778137 genotypes and pathological complete response (pCR, defined that the postoperative pathology indicating no residual invasive breast cancer in the breast or the axillary lymph node) were analyzed. Logistic regression analysis was performed to identify the independent predictors of pCR. Survival was assessed by Kaplan-Meier method and Cox regression analysis according to the rs4778137 genotypes. RESULTS: The differences between pCR and the rs4778137 genotypes were statistically significant (p < 0.05). The patients with genotype GG harbored a better disease-free survival (HR: 2.358, p = 0.000) and overall survival (HR: 1.578, p = 0.008) than the patients with genotype CC in rs4778137. The further Univariate and Multivariate survival analysis revealed that SNP rs4778137 was an independent predictive factor of disease-free survival (p = 0.000/p = 0.001) and overall survival (p = 0.006/p = 0.045). CONCLUSION: The OCA2 rs4778137 may be a predictor for the clinical response and survival in local advanced breast cancer patients who received neoadjuvant chemotherapy.


Assuntos
Antineoplásicos/uso terapêutico , Neoplasias da Mama/genética , Neoplasias da Mama/terapia , Proteínas de Membrana Transportadoras/genética , Terapia Neoadjuvante , Polimorfismo de Nucleotídeo Único , Adulto , Neoplasias da Mama/mortalidade , Quimioterapia Adjuvante , Estudos de Coortes , Terapia Combinada , Epirubicina/uso terapêutico , Feminino , Seguimentos , Humanos , Pessoa de Meia-Idade , Prognóstico , Análise de Sobrevida , Taxoides/uso terapêutico , Adulto Jovem
20.
Zhongguo Dang Dai Er Ke Za Zhi ; 19(7): 812-815, 2017 Jul.
Artigo em Chinês | MEDLINE | ID: mdl-28697837

RESUMO

The study reports a female neonate with a gestational age of 29+2 weeks and a birth weight of 1 210 g. Ten minutes after birth, the neonate was admitted to the hospital due to shortness of breath. Several days after birth, the neonate presented with hyperglycemia, polyuria, and poor weight gain, accompanied by azotemia, hypochloremic metabolic alkalosis, hypokalemia, and hyponatremia. Laboratory examinations showed elevated levels of aldosterone, renin, and angiotensin II. Gene detection revealed SLC12A1 gene mutation. Neonatal Bartter syndrome was thus confirmed. The neonate was treated with sodium and potassium supplements, and was followed up for 8 months. During the follow-up, the mental and neural development of the neonate was almost normal at the corrected age, and regular reexaminations showed slight metabolic alkalosis and almost normal electrolyte levels. For the neonates who have the symptoms of unexplainable polyurine and electrolyte disorders, it is important to examine the levels of aldosterone, renin and angiotensin. A definite diagnosis of neonatal Bartter syndrome can be made based on the presence of SLC12A1 gene mutation.


Assuntos
Acidose/etiologia , Síndrome de Bartter/etiologia , Hipopotassemia/etiologia , Ganho de Peso , Síndrome de Bartter/terapia , Feminino , Humanos , Recém-Nascido , Recidiva
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