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1.
Nat Commun ; 11(1): 340, 2020 Jan 17.
Artigo em Inglês | MEDLINE | ID: mdl-31953413

RESUMO

Mikania micrantha is one of the top 100 worst invasive species that can cause serious damage to natural ecosystems and substantial economic losses. Here, we present its 1.79 Gb chromosome-scale reference genome. Half of the genome is composed of long terminal repeat retrotransposons, 80% of which have been derived from a significant expansion in the past one million years. We identify a whole genome duplication event and recent segmental duplications, which may be responsible for its rapid environmental adaptation. Additionally, we show that M. micrantha achieves higher photosynthetic capacity by CO2 absorption at night to supplement the carbon fixation during the day, as well as enhanced stem photosynthesis efficiency. Furthermore, the metabolites of M. micrantha can increase the availability of nitrogen by enriching the microbes that participate in nitrogen cycling pathways. These findings collectively provide insights into the rapid growth and invasive adaptation.

2.
Arch Insect Biochem Physiol ; 102(3): e21613, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31549439

RESUMO

Frankliniella occidentalis is an economically important invasive pest worldwide, which can damage various horticultural crops and ornamental plants. F. occidentalis was first intercepted in Kunming, Yunnan province in 2000, and first reported to establish a population in Beijing, China in 2003. Since then, this pest is currently distributed across tens of provinces in mainland China and cause increasingly serious damage and loss. To control this pest, invasion biology, monitoring, and integrated pest management have been generally and intensively studied for 15 years in China. Furthermore, western flower thrips (WFT) as an important invasive insect pest, the research achievements on WFT has contributed to the promotion of technological innovation and development for invasive alien species management strategies and techniques in China. This review provides an overview for research on the biology, ecology, prevention, and management of this pest during 15 years in China. Meanwhile, China's "4E action" strategy on F. occidentalis is also discussed in this review.


Assuntos
Controle de Insetos/métodos , Tisanópteros/fisiologia , Animais , China , Genética Populacional , Resistência a Inseticidas , Inseticidas , Espécies Introduzidas , Tisanópteros/genética
3.
Nat Commun ; 10(1): 4237, 2019 09 17.
Artigo em Inglês | MEDLINE | ID: mdl-31530873

RESUMO

The codling moth Cydia pomonella, a major invasive pest of pome fruit, has spread around the globe in the last half century. We generated a chromosome-level scaffold assembly including the Z chromosome and a portion of the W chromosome. This assembly reveals the duplication of an olfactory receptor gene (OR3), which we demonstrate enhances the ability of C. pomonella to exploit kairomones and pheromones in locating both host plants and mates. Genome-wide association studies contrasting insecticide-resistant and susceptible strains identify hundreds of single nucleotide polymorphisms (SNPs) potentially associated with insecticide resistance, including three SNPs found in the promoter of CYP6B2. RNAi knockdown of CYP6B2 increases C. pomonella sensitivity to two insecticides, deltamethrin and azinphos methyl. The high-quality genome assembly of C. pomonella informs the genetic basis of its invasiveness, suggesting the codling moth has distinctive capabilities and adaptive potential that may explain its worldwide expansion.


Assuntos
Cromossomos de Insetos/genética , Resistência a Inseticidas , Inseticidas/farmacologia , Mariposas/efeitos dos fármacos , Mariposas/genética , Animais , Duplicação Gênica , Genoma de Inseto , Proteínas de Insetos/genética , Proteínas de Insetos/metabolismo , Mariposas/metabolismo , Feromônios/metabolismo , Polimorfismo de Nucleotídeo Único , Regiões Promotoras Genéticas , Receptores Odorantes/genética , Receptores Odorantes/metabolismo
4.
J Proteomics ; 207: 103465, 2019 Sep 15.
Artigo em Inglês | MEDLINE | ID: mdl-31344497

RESUMO

Protein lysine acetylation is a reversible posttranslational modification and plays a pivotal role in a broad array of physiological functions. In our study, a strategy combining immunoaffinity enrichment of acetylated peptides based on anti-acetyllysine antibody with high-resolution tandem mass spectrometry was employed for a systemic survey of acetylation sites in a polyphagous pest insect Thrips tabaci. In total, 597 acetylated proteins containing 995 lysine acetylation sites were identified in T. tabaci. Interestingly, functional enrichment analysis showed that acetylated proteins are implicated in the regulation of diverse KEGG pathways, including carbohydrate metabolism, energy metabolism, amino acid metabolism, and translational process. In particular, a large fraction of metabolic enzymes, including multiple rate-limiting enzymes, was also found to be acetylated. Comparative analysis indicated that a proportion of euNOG entries was shared by three insects. Furthermore, motif analysis showed that the sequence flanking acetylation sites exhibited subcellular compartment-specific patterns. Protein-protein interaction network analysis demonstrated that acetylated proteins formed several densely connected sub-networks tightly associated with ribosome, fatty acid metabolism, oxidative phosphorylation and purine metabolism, thus strengthening the functional enrichment result. Overall, our study provides a comprehensive view of acetylation sites, facilitating an in-depth investigation of functional roles of acetylation in the future. SIGNIFICANCE: Onion thrips is a polyphagous agricultural pest insect. Insecticide resistance has been frequently reported due to the intensive use of chemical pesticides. Lysine acetylation is a ubiquitous posttranslational modification and plays important roles in gene regulation. An in-depth understanding of transcriptional regulation is crucial for designing novel and highly efficient pesticides. With high-resolution mass spectrometry based proteomics method, we systematically explored the acetylome in this insect. In total, 595 proteins containing 995 acetylation sites were identified in this study. Bioinformatic analysis revealed that acetylated proteins are implicated in regulating diverse biological processes, including carbohydrate metabolism, energy metabolism, amino acid metabolism, and translational process. Furthermore, protein-protein interaction network analysis showed that ribosome, fatty acid metabolism, oxidative metabolism and purine metabolism are significantly enriched for acetylated proteins. Our results provide insights into the targets of acetylation in onion thrips and facilitate elucidation of transcriptional regulation and design of novel control strategies against this insect.

5.
Gigascience ; 7(9)2018 09 01.
Artigo em Inglês | MEDLINE | ID: mdl-30107526

RESUMO

Background: The golden apple snail (Pomacea canaliculata) is a freshwater snail listed among the top 100 worst invasive species worldwide and a noted agricultural and quarantine pest that causes great economic losses. It is characterized by fast growth, strong stress tolerance, a high reproduction rate, and adaptation to a broad range of environments. Results: Here, we used long-read sequencing to produce a 440-Mb high-quality, chromosome-level assembly of the P. canaliculata genome. In total, 50 Mb (11.4%) repeat sequences and 21,533 gene models were identified in the genome. The major findings of this study include the recent explosion of DNA/hAT-Charlie transposable elements, the expansion of the P450 gene family, and the constitution of the cellular homeostasis system, which contributes to ecological plasticity in stress adaptation. In addition, the high transcriptional levels of perivitelline genes in the ovary and albumen gland promote the function of nutrient supply and defense ability in eggs. Furthermore, the gut metagenome also contains diverse genes for food digestion and xenobiotic degradation. Conclusions: These findings collectively provide novel insights into the molecular mechanisms of the ecological plasticity and high invasiveness.


Assuntos
Aclimatação/genética , Genoma , Espécies Introduzidas , Caramujos/genética , Estresse Fisiológico/genética , Animais
6.
Sheng Wu Gong Cheng Xue Bao ; 33(3): 486-493, 2017 Mar 25.
Artigo em Chinês | MEDLINE | ID: mdl-28941346

RESUMO

The contradiction between the increasing population and the decrease of tillable land areas is becoming more and more serious in our country. Food security is an important guarantee for sustainable development of our national economy. Photosynthesis is the basis for crop yield. Improving crop photosynthetic efficiency is one of the important approaches to increase crop yield. In this review, we summarized the recent advances in engineering photosynthetic performance by synthetic biology from three key aspects including absorption, transduction and conversion of light energy, light utilization efficiency and carbon assimilation. We also addressed the prospects of its application in increasing photosynthetic efficiency through synthetic biology principles, which may provide important theoretical support and key biotechnology to increase grain production.


Assuntos
Produtos Agrícolas/fisiologia , Fotossíntese , Biologia Sintética , Biotecnologia , Carbono/metabolismo , Produtos Agrícolas/crescimento & desenvolvimento
7.
Autophagy ; 13(8): 1318-1330, 2017 Aug 03.
Artigo em Inglês | MEDLINE | ID: mdl-28594263

RESUMO

Magnaporthe oryzae, the ascomycete fungus that causes rice blast disease, initiates conidiation in response to light when grown on Prune-Agar medium containing both carbon and nitrogen sources. Macroautophagy/autophagy was shown to be essential for M. oryzae conidiation and induced specifically upon exposure to light but is undetectable in the dark. Therefore, it is inferred that autophagy is naturally induced by light, rather than by starvation during M. oryzae conidiation. However, the signaling pathway(s) involved in such phototropic induction of autophagy remains unknown. We identified an M. oryzae ortholog of GCN5 (MGG_03677), encoding a histone acetyltransferase (HAT) that negatively regulates light- and nitrogen-starvation-induced autophagy, by acetylating the autophagy protein Atg7. Furthermore, we unveiled novel regulatory mechanisms on Gcn5 at both transcriptional and post-translational levels, governing its function associated with the unique phototropic response of autophagy in this pathogenic fungus. Thus, our study depicts a signaling network and regulatory mechanism underlying the autophagy induction by important environmental clues such as light and nutrients.


Assuntos
Autofagia , Biocatálise , Proteínas Fúngicas/metabolismo , Magnaporthe/citologia , Magnaporthe/metabolismo , Processos Fototróficos , Acetilação , Autofagia/efeitos da radiação , Regulação Fúngica da Expressão Gênica/efeitos da radiação , Genes Fúngicos , Luz , Magnaporthe/genética , Magnaporthe/efeitos da radiação , Processos Fototróficos/efeitos da radiação , Ligação Proteica , Processamento de Proteína Pós-Traducional/efeitos da radiação , Esporos Fúngicos/metabolismo , Esporos Fúngicos/efeitos da radiação , Transcrição Genética/efeitos da radiação
8.
J Exp Bot ; 66(9): 2709-21, 2015 May.
Artigo em Inglês | MEDLINE | ID: mdl-25788731

RESUMO

The mycotoxin fumonisin B1 (FB1) is a strong inducer of programmed cell death (PCD) in plants, but its underlying mechanism remains unclear. Here, we describe two ubiquitin ligases, RING DOMAIN LIGASE3 (RGLG3) and RGLG4, which control FB1-triggered PCD by modulating the jasmonate (JA) signalling pathway in Arabidopsis thaliana. RGLG3 and RGLG4 transcription was sensitive to FB1. Arabidopsis FB1 sensitivity was suppressed by loss of function of RGLG3 and RGLG4 and was increased by their overexpression. Thus RGLG3 and RGLG4 have coordinated and positive roles in FB1-elicited PCD. Mutated JA perception by coi1 disrupted the RGLG3- and RGLG4-related response to FB1 and interfered with their roles in cell death. Although FB1 induced JA-responsive defence genes, it repressed growth-related, as well as JA biosynthesis-related, genes. Consistently, FB1 application reduced JA content in wild-type plants. Furthermore, exogenously applied salicylic acid additively suppressed JA signalling with FB1 treatment, suggesting that FB1-induced salicylic acid inhibits the JA pathway during this process. All of these effects were attenuated in rglg3 rglg4 plants. Altogether, these data suggest that the JA pathway is hijacked by the toxin FB1 to elicit PCD, which is coordinated by Arabidopsis RGLG3 and RGLG4.


Assuntos
Apoptose/fisiologia , Proteínas de Arabidopsis/fisiologia , Arabidopsis/fisiologia , Ciclopentanos/metabolismo , Fumonisinas/farmacologia , Ligases/fisiologia , Oxilipinas/metabolismo , Domínios RING Finger , Transdução de Sinais , Apoptose/efeitos dos fármacos , Arabidopsis/citologia , Arabidopsis/efeitos dos fármacos , Proteínas de Arabidopsis/genética , Proteínas de Arabidopsis/metabolismo , Núcleo Celular/metabolismo , Citoplasma/metabolismo , Regulação da Expressão Gênica de Plantas , Ligases/genética , Ligases/metabolismo , Ácido Salicílico/metabolismo
9.
Plant Physiol ; 160(2): 808-22, 2012 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-22898498

RESUMO

Jasmonates (JAs) regulate various stress responses and development processes in plants, and the JA pathway is tightly controlled. In this study, we report the functional characterization of two novel RING-type ubiquitin ligases, RING DOMAIN LIGASE3 (RGLG3) and RGLG4, in modulating JA signaling. Both RGLG3 and RGLG4 possessed ubiquitin ligase activities and were widely distributed in Arabidopsis (Arabidopsis thaliana) tissues. Altered expression of RGLG3 and RGLG4 affected methyl JA-inhibited root growth and JA-inductive gene expression, which could be suppressed by the coronatine insensitive1 (coi1) mutant. rglg3 rglg4 also attenuated the inhibitory effect of JA-isoleucine-mimicking coronatine on root elongation, and consistently, rglg3 rglg4 was resistant to the coronatine-secreting pathogen Pseudomonas syringae pv tomato DC3000, suggesting that RGLG3 and RGLG4 acted in response to the coronatine and promoted JA-mediated pathogen susceptibility. In addition, rglg3 rglg4 repressed wound-stunted plant growth, wound-stimulated expression of JA-responsive genes, and wound-induced JA biosynthesis, indicating their roles in JA-dependent wound response. Furthermore, both RGLG3 and RGLG4 responded to methyl JA, P. syringae pv tomato DC3000, and wounding in a COI1-dependent manner. Taken together, these results indicate that the ubiquitin ligases RGLG3 and RGLG4 are essential upstream modulators of JA signaling in response to various stimuli.


Assuntos
Acetatos/farmacologia , Proteínas de Arabidopsis/metabolismo , Arabidopsis/enzimologia , Ciclopentanos/farmacologia , Regulação da Expressão Gênica de Plantas , Oxilipinas/farmacologia , Ubiquitina-Proteína Ligases/metabolismo , Aminoácidos/metabolismo , Arabidopsis/efeitos dos fármacos , Arabidopsis/genética , Arabidopsis/microbiologia , Proteínas de Arabidopsis/genética , Proteínas de Bactérias/metabolismo , Ativação Enzimática , Retroalimentação Fisiológica , Regulação Enzimológica da Expressão Gênica , Genes de Plantas , Indenos/metabolismo , Filogenia , Doenças das Plantas/microbiologia , Imunidade Vegetal , Raízes de Plantas/efeitos dos fármacos , Raízes de Plantas/enzimologia , Raízes de Plantas/crescimento & desenvolvimento , Plantas Geneticamente Modificadas/enzimologia , Plantas Geneticamente Modificadas/genética , Plantas Geneticamente Modificadas/crescimento & desenvolvimento , Pseudomonas syringae/patogenicidade , Proteínas Recombinantes/genética , Proteínas Recombinantes/metabolismo , Transdução de Sinais , Ubiquitina-Proteína Ligases/genética , Ubiquitinação
10.
Biochem Biophys Res Commun ; 364(3): 668-74, 2007 Dec 21.
Artigo em Inglês | MEDLINE | ID: mdl-18028877

RESUMO

Miniature inverted-repeat transposable elements (MITEs) have a broad impact on genome structure and function. Although MITEs are found associated to genes, little is known about their effect on gene regulation. We have identified a novel MITE family, named Triton, whilst analyzing two independent trichosanthin (TCS) gene promoters (TP9 and TP12) cloned from Trichosanthes kirilowii Maximowicz. Triton1 and Triton2 are nested in TP9, and Triton3 (with 93% sequence similarity to Triton2) is in TP12. To assess the effect of MITE insertion on TCS promoters, we excised Triton1 from TP9 and inserted it into TP12. GUS activity analysis revealed that nested Triton1 is required for effective repression of promoter activity. Detailed analyses of a series of 5'-truncated promoters concerning Triton1 showed that a dark-specific repressor and some constitutive elements endow Triton1 with ability to response to light conditions. These results suggest that Triton1 MITE, which contains cis-regulatory elements, could mediate gene expression.


Assuntos
Elementos de DNA Transponíveis/genética , Regulação da Expressão Gênica de Plantas/genética , Trichosanthes/genética , Sequência de Bases , DNA de Plantas/genética , DNA de Plantas/isolamento & purificação , Escuridão , Genes de Plantas , Dados de Sequência Molecular , Filogenia , Regiões Promotoras Genéticas/genética , Sítio de Iniciação de Transcrição
11.
Plant Cell Rep ; 26(1): 85-93, 2007 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-16924502

RESUMO

With the use of in vivo recombination theory, the screening time of yeast one-hybrid system was decreased in the present study. A basic helix-loop-helix (bHLH) protein PsGBF was successfully obtained from a glutathione (GSH)-induced pea cDNA library using the G-box cis-element of the PsCHS1 promoter as a bait. Electrophoretic mobility shift assay (EMSA) and beta-galactosidase assay results suggested that PsGBF possesses both G-box-specific binding and transcription-activating activities. The specific interaction of PsGBF with G-box was further confirmed by in vivo transient expression assays in tobacco. The current study examined the combination effect of G-box with Box I elements in the interaction with PsGBF or OsMYC. The results indicated that PsGBF bound with the G-box, but not the Box I element. Moreover, this combination effect of G-box and Box I only associated with PsGBF but not with other bHLH-type proteins such as OsMYC.


Assuntos
Aciltransferases/genética , Fatores de Ligação G-Box/genética , Sequências Hélice-Alça-Hélice/genética , Regiões Promotoras Genéticas/genética , Sequência de Aminoácidos , Sequência de Bases , Northern Blotting , Proteínas de Ligação a DNA/genética , Proteínas de Ligação a DNA/metabolismo , Ensaio de Desvio de Mobilidade Eletroforética , Fatores de Ligação G-Box/metabolismo , Regulação da Expressão Gênica de Plantas/efeitos dos fármacos , Glutationa/farmacologia , Modelos Genéticos , Dados de Sequência Molecular , Ervilhas/genética , Ervilhas/metabolismo , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Ligação Proteica , Homologia de Sequência de Aminoácidos , Tabaco/genética , Tabaco/metabolismo , Transcrição Genética/efeitos dos fármacos , Técnicas do Sistema de Duplo-Híbrido
12.
Front Biosci ; 12: 1670-9, 2007.
Artigo em Inglês | MEDLINE | ID: mdl-17129850

RESUMO

Chaltone synthase (CHS) is a key speed-limiting enzyme in the phenylpropanoid pathway which plays an important role in plant defense response against pathogens. In the PsCHS1 promoter, there is an AT-rich element (ATRE) which is required for the maximal elicitor-mediated activation. However, the transcription activator of the ATRE and its regulation mechanism in pea keep unclear. In this paper, a new ATRE-binding factor was isolated from an elicitor-induced pea cDNA expression library and was designated as PsATF1. Electrophoretic mobility shift assay (EMSA) indicated the ATRE-specific binding activity of PsATF1. Beta-galactosidase assays in yeast cells suggested that PsATF1 possessed transcription-activating activity because PsATF1 activated the expression of the reporter gene even without the GAL4 activation domain (AD). The current study also examined the co-activation effects of PsATF1 with another transcription factor PsGBF on ATRE or PsCHS1 promoter through a transient expression system. The present work reports that PsATF1 acts as a complete transcription activator and first indicates that there are combined effects of PsATF1 with PsGBF on the activation of PsCHS1 promoter. These results provide theoretical basis to the plant defense gene expression mechanism regulated by multiple activators.


Assuntos
Aciltransferases/genética , Regulação da Expressão Gênica de Plantas , Ervilhas/genética , Proteínas de Plantas/metabolismo , Elementos de Resposta , Transativadores/metabolismo , Sequência Rica em At , Sequência de Aminoácidos , Sequência de Bases , Sítios de Ligação , Proteínas de Ligação a DNA/metabolismo , Fatores de Ligação G-Box/metabolismo , Glutationa/farmacologia , Dados de Sequência Molecular , Proteínas de Plantas/química , Proteínas de Plantas/genética , Regiões Promotoras Genéticas , Transativadores/química , Transativadores/genética , Ativação Transcricional
13.
Eur J Immunol ; 36(1): 199-206, 2006 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-16323247

RESUMO

Toll-like receptor 3 (TLR3) plays an important role in antiviral responses through recognizing viral double-stranded RNA produced during viral infection and mediating induction of type I IFN. TRIF is a Toll/IL-1 receptor (TIR) domain-containing adaptor protein that is associated with TLR3 and critically involved in TLR3-mediated signaling. In yeast two-hybrid screens, we identified TNF receptor-associated factor (TRAF)1 as a TRIF-interacting protein. The TRAF-C domain of TRAF1 and the TIR domain of TRIF were responsible for their interaction. Overexpression of TRAF1 inhibited TRIF- and TLR3-mediated activation of NF-kappaB, IFN-stimulated response element and the IFN-beta promoter. Overexpression of TRIF caused caspase-dependent cleavage of TRAF1. The cleaved N-terminal but not C-terminal fragment of TRAF1 was responsible for inhibiting TRIF signaling. Mutation of the caspase cleavage site of TRAF1 or addition of the caspase inhibitor crmA inhibited TRAF1 cleavage and abolished the ability of TRAF1 to inhibit TRIF signaling, suggesting that TRIF-induced cleavage of TRAF1 is required for its inhibition of TRIF signaling. Our findings provide a novel mechanism for negative regulation of TRIF-mediated signaling.


Assuntos
Proteínas Adaptadoras de Transporte Vesicular/metabolismo , Receptores de Interleucina-1/metabolismo , Transdução de Sinais/imunologia , Fator 1 Associado a Receptor de TNF/metabolismo , Proteínas Adaptadoras de Transporte Vesicular/imunologia , Western Blotting , Linhagem Celular , Eletroforese em Gel de Poliacrilamida , Humanos , Interferon beta/imunologia , Interferon beta/metabolismo , NF-kappa B/imunologia , NF-kappa B/metabolismo , Receptores de Interleucina-1/imunologia , Fator 1 Associado a Receptor de TNF/imunologia , Receptor 3 Toll-Like/imunologia , Receptor 3 Toll-Like/metabolismo , Transfecção , Técnicas do Sistema de Duplo-Híbrido
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