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1.
Food Chem ; 372: 130935, 2022 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-34818725

RESUMO

The impacts of cold stress (4 â„ƒ for 0 h, 12 h, 24 h, 36 h and 48 h, respectively) on the components, structural and physical properties of myofibrillar protein (MP) gel from Procambarus clarkii were investigated. The physicochemical analysis indicated the secondary and tertiary structure of MP were unfolding to different degrees after cold stress when compared to the control. The MP gel hardness reached a maximum when the cold stress reached 24 h. Furthermore, the quantitative proteomics results indicated that 20 up-regulated differentially abundant proteins (DAPs) were detected in 24 h when compared to control, specifically include myosin light chain 1 (MLC1) and skeletal muscle actin 6. Additionally, the combined analysis confirmed that MLC1 and skeletal muscle actin 6 might play key roles in hardening shrimp meat under cold stress. The results could provide a theoretical reference for the changes in crayfish muscle quality during cold chain transportation.


Assuntos
Astacoidea , Proteômica , Animais , Astacoidea/genética , Resposta ao Choque Frio , Músculos
2.
Spectrochim Acta A Mol Biomol Spectrosc ; 265: 120370, 2022 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-34536887

RESUMO

In this work, a serials of PS(polystyrene)/Cu2S/Ag sandwich substrates were successfully constructed using the magnetic sputtering method by adjusting the Ag sputtering time (0 min, 2 min, 4 min, 6 min, 8 min and 10 min) and used as the surface-enhanced Raman scattering (SERS) substrates. When the Ag sputtering time was 6 min, the strongest SERS signal was observed. The optimized SERS substrate has strong SERS activity on 4-mercaptobenzoic acid (4-MBA), the minimum detection limit was 10-13 M and the enhancement factor was as high as 4.7 × 107. In addition, the SERS signals were highly reproducible with small standard deviation. The SERS enhancement mechanism of the PS/Cu2S/Ag system was attributed to the synergistic effect of the chemical mechanism and the electromagnetic enhancement mechanism. This strategy has find a new way for manufacturing SERS activity sensor with high sensitivity and reproducibility.


Assuntos
Nanopartículas Metálicas , Prata , Reprodutibilidade dos Testes , Análise Espectral Raman
3.
BMC Cardiovasc Disord ; 21(1): 574, 2021 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-34852763

RESUMO

BACKGROUND: We performed a meta-analysis sought to investigate the risk of stroke with antiplatelet and anticoagulant therapies among patients with coronary artery disease (CAD). METHODS: We searched PubMed, EMBASE, and Cochrane Library for randomized controlled trials from January 1995 to March 2020. Studies were retrieved if they reported data of stroke for patients with CAD and were randomized to receive intensive versus conservative antithrombotic therapies, including antiplatelet and oral anticoagulant (OAC). Analyses were pooled by random-effects modeling. A total of 42 studies with 301,547subjects were enrolled in this analysis. RESULTS: Intensive antithrombotic therapy significantly reduced risk of all stroke (RR 0.86, 95% CI 0.80-0.94) and ischemic stroke (RR 0.80, 95% CI 0.71-0.91), but increased risk of hemorrhagic stroke (RR 1.36, 95% CI 1.00-1.86) and intracranial hemorrhage (RR 1.46, 95% CI 1.17-1.81). Subgroup analyses indicated that OAC yields more benefit to all stroke than antiplatelet therapy (OAC: RR 0.73, 95% CI 0.58-0.92; Antiplatelet: RR 0.90, 95% CI 0.83-0.97; Between-group heterogeneity P value = 0.030). The benefit of antiplatelet therapy on all stroke and ischemic stroke were mainly driven by the studies comparing longer versus shorter duration of dual antiplatelet therapy (All stroke: RR 0.86, 95% CI 0.78-0.95; ischemic stroke: RR 0.84, 95% CI 0.75-0.94). CONCLUSIONS: Among CAD patients who have already received antiplatelet therapy, either strengthening antiplatelet or anticoagulant treatments significantly reduced all stroke, mainly due to the reduction of ischemic stroke, although it increased the risk of hemorrhagic stroke and intracranial hemorrhage. OAC yields more benefit to all stroke than antiplatelet therapy.

4.
Natl Sci Rev ; 8(10): nwaa287, 2021 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-34858601

RESUMO

As a promising low-cost energy storage device, the development of a rechargeable potassium-ion battery (KIB) is severely hindered by the limited capacity of cathode candidates. Regarded as an attractive capacity-boosting strategy, triggering the O-related anionic redox activity has not been achieved within a sealed KIB system. Herein, in contrast to the typical gaseous open K-O2 battery (O2/KO2 redox), we originally realize the reversible superoxide/peroxide (KO2/K2O2) interconversion on a KO2-based cathode. Controlled within a sealed cell environment, the irreversible O2 evolution and electrolyte decomposition (induced by superoxide anion (O2 -) formation) are effectively restrained. Rationally controlling the reversible depth-of-charge at 300 mAh/g (based on the mass of KO2), no obvious cell degradation can be observed during 900 cycles. Moreover, benefitting from electrolyte modification, the KO2-based cathode is coupled with a limited amount of K-metal anode (merely 2.5 times excess), harvesting a K-metal full-cell with high energy efficiency (∼90%) and long-term cycling stability (over 300 cycles).

5.
Food Chem ; 373(Pt B): 131478, 2021 Oct 29.
Artigo em Inglês | MEDLINE | ID: mdl-34731791

RESUMO

Microbial infection, senescence and water losses result in serious quality deterioration of postharvest mushrooms. The aim of this study was to investigate the impact of ultrasound treatment (US), gamma irradiation treatment (GI) and their combination on quality maintenance of fresh Lentinula edodes during storage. The results showed that US + GI was the most effective approach to maintaining the quality of mushrooms. US + GI reduced natural microflora present on L. edodes, such as total number of colonies, molds, yeasts, Pseudomonas and Enterobacteriaceae. Furthermore, US + GI stimulated phenylalanine ammonia lyase, maintained the highest level of total phenolic content (733.63 mg GAE/kg on Day 4), and postponed the occurrence of reduced ascorbic acid (33.7% retention relative to the control), which contributed to strengthening the antioxidant capacity. Additionally, US + GI retarded water mobility and loss. In brief, the US + GI in this study is an effective hurdle technology for preserving the quality of fresh L. edodes during storage.

6.
ACS Appl Mater Interfaces ; 13(46): 55700-55711, 2021 Nov 24.
Artigo em Inglês | MEDLINE | ID: mdl-34752083

RESUMO

Currently, lithium-ion batteries (LIBs) are assembled with polar electrolytes; thus, resulting SEI layers are dominated with organics. Herein, a low-polarity electrolyte is formulated with a low-polarity solvent (tetraethyl silicate, TEOS) and a non-polar inert shielding co-solvent (cyclohexane, CYH); solvation behaviors of lithium salt are investigated. The use of such a low-polarity solvent is found to improve the fraction of anions in the solvation sheath of Li+, and the presence of the non-polar co-solvent further shields the reductive decomposition of the solvent on the anode. The resulting SEI layer is relatively rich in LiF and has a 3D cross-linked Si-O network as a skeleton from the decomposition of TEOS molecules, which is more robust to tolerate the damage from the volume expansion of silicon. A Si-nanoparticle-based anode in such a low-polarity electrolyte delivers a capacity as high as 1491 mAh g-1 after 200 cycles, outperforming those in the commercial polar electrolytes.

7.
Entropy (Basel) ; 23(11)2021 Oct 31.
Artigo em Inglês | MEDLINE | ID: mdl-34828138

RESUMO

Quantum key distribution (QKD) is a promising technique to share unconditionally secure keys between remote parties. As an essential part of a practical QKD system, reconciliation is responsible for correcting the errors due to the quantum channel noise by exchanging information through a public classical channel. In the present work, we propose a novel syndrome-based low-density parity-check (LDPC) reconciliation protocol to reduce the information leakage of reconciliation by fully utilizing the syndrome information that was previously wasted. Both theoretical analysis and simulation results show that our protocol can evidently reduce the information leakage as well as the number of communication rounds.

8.
Artigo em Inglês | MEDLINE | ID: mdl-34797761

RESUMO

Interactive image restoration aims to generate restored images by adjusting a controlling coefficient which determines the restoration level. Previous works are restricted in modulating image with a single coefficient. However, real images always contain multiple types of degradation, which cannot be well determined by one coefficient. This paper presents a new problem setup, called multi-dimension (MD) modulation, which aims at modulating output effects across multiple degradation types and levels. Compared with the previous single-dimension (SD) modulation, the MD setup to handle multiple degradations adaptively and relief data unbalancing problem in different degradation types. We also propose a deep architecture - CResMD with newly introduced controllable residual connections. Specifically, we add a controlling variable on the conventional residual connection to allow a weighted summation of input and residual. The values of these weights are generated by another condition network. We further propose a new data sampling strategy based on beta distribution together with a simple loss reweighting approach to balance different degradation types and levels. With corrupted image and degradation information as inputs, the network can output the corresponding restored image. By tweaking the condition vector, users can control the output effects in MD space at test time.

9.
Artigo em Inglês | MEDLINE | ID: mdl-34752396

RESUMO

We present a novel two-stage approach for automated floorplan design in residential buildings with a given exterior wall boundary. Our approach has the unique advantage of being human-centric, that is, the generated floorplans can be geometrically plausible, as well as topologically reasonable to enhance resident interaction with the environment. From the input boundary, we first synthesize a human-activity map that reflects both the spatial configuration and human-environment interaction in an architectural space. We propose to produce the human-activity map either automatically by a pre-trained generative adversarial network (GAN) model, or semi-automatically by synthesizing it with user manipulation of the furniture. Second, we feed the human-activity map into our deep framework ActFloor-GAN to guide a pixel-wise prediction of room types. We adopt a re-formulated cycle-consistency constraint in ActFloor-GAN to maximize the overall prediction performance, so that we can produce high-quality room layouts that are readily convertible to vectorized floorplans. Experimental results show several benefits of our approach. First, a quantitative analysis of ablated techniques shows superior performance of leveraging the human-activity map in predicting piecewise room types. Second, a subjective evaluation by architects shows that our results have compelling quality as professionally-designed floorplans and much better than those generated by existing methods in terms of the room layout topology. Last, our approach allows manipulating the furniture placement, considers the human activities in the environment, and enables the incorporation of user-design preferences.

10.
Ecotoxicol Environ Saf ; 227: 112916, 2021 Dec 20.
Artigo em Inglês | MEDLINE | ID: mdl-34695613

RESUMO

High ambient temperature-induced heat stress (HS) during pregnancy may affect the placental function and fetal development. Late gestation is a critical period of the developing fetal brain and intestine. The study aimed to investigate the effects of HS during late pregnancy on the function of placenta, fetal brain and intestine in a mouse model. We found that the number of stillborn fetal mice were increased due to maternal HS. Transcriptome analysis revealed that the expression of genes enriched in nutrients transport and metabolism of HS group were up-regulated in the placenta, but down-regulated in the fetal duodenum and jejunum. Interestingly, the concentration of triglyceride (TG) in the HS group was raised in the placenta, but reduced both in the fetal duodenum and jejunum compared with the thermal-neutral (TN) group. Additionally, maternal HS also reduced total cholesterol (TC) contents in the fetal duodenum. The mRNA expression and protein levels of placental fatty acid binding protein 2 and 4 (fabp2 and fabp4) were not affected by maternal HS, but the mRNA expression and protein levels of cluster of differentiation 36 (CD36) and diacylglycerol acyltransferase-2 (Dgat2) were decreased in the fetal intestine. Furthermore, maternal HS reduced the mRNA expression and protein levels of the placental 11beta-hydroxysteroid dehydrogenase type 2 (Hsd11b2) and 5-hydroxytryptamine receptor 1D (Htr1d). The concentrations of corticosterone and the expression of heat shock protein 90 beta family member 1 (hsp90b1), hypoxia up-regulated 1 (hyou1) and corticotropin releasing hormone receptor 1 (crhr1) enriched in response to glucocorticoids in the fetal brain were increased by maternal HS. Taken together, our findings demonstrated that maternal HS disrupted the placental glucocorticoid barrier and serotonin system associated with the raised corticosterone levels in the fetal brain, which might contribute to the decreased capacity of nutrients transport and metabolism in the fetal intestine.


Assuntos
11-beta-Hidroxiesteroide Desidrogenase Tipo 2 , Placenta , 11-beta-Hidroxiesteroide Desidrogenase Tipo 2/metabolismo , Animais , Encéfalo/metabolismo , Feminino , Feto , Resposta ao Choque Térmico , Camundongos , Placenta/metabolismo , Gravidez
11.
Sci Total Environ ; : 150997, 2021 Oct 14.
Artigo em Inglês | MEDLINE | ID: mdl-34656588

RESUMO

This study reports the transformation behavior of nitrogen during the co-hydrothermal carbonization of sewage sludge and model compounds (microcrystalline cellulose, starch, lignin, and xylan) of food waste at 220 °C, with a focus on the reaction routes between starch/xylan and NH4+. Most of the nitrogen in the raw sludge was transformed into organic-N (44.6%) and NH4+ (23.3%) in the aqueous product, and only 20.3% of nitrogen was retained in the hydrochar. The added model compounds could react with organic-N (i.e., amino acids and amines) and NH4+ in aqueous products through Maillard and Mannich reactions, generating heterocyclic-N (especially pyrrole-N) which further polymerizes to form nitrogen-containing polyaromatic hydrochar. This leads to an increase in the retention rate of nitrogen to 36.8-50.9%, especially upon the addition of starch and xylan. During the hydrothermal carbonization of starch/xylan in the NH4+ solution, the polymers are first hydrolyzed into monomers, followed by their further reaction with NH4+ to generate pyrrole-N and pyridine-N in aqueous products (especially xylan), and the pyrrole-N can then polymerize with aromatic clusters to form hydrochar-N. The results show that the model compounds of food waste substantially affect the nitrogen transformation pathways during hydrothermal carbonization, mainly because of the structures of their monomers. These findings can guide the production of sludge-based hydrochar with the targeted regulation of nitrogen content and species.

12.
Phytother Res ; 35(11): 6310-6323, 2021 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-34514657

RESUMO

Overactivation of TGF-ß/ALK5/Smad signaling pathway has been observed in the advanced stage of various human malignancies. As a key component of TGF-ß/ALK5/Smad signaling pathway transduction, TGF-ß type I receptor (also known as ALK5) has emerged as a promising therapeutic target for cancer treatment. In this study, to discover a novel ALK5 inhibitor, a commercial natural products library was screened using docking-based virtual screening, followed by luciferase reporter assay. A flavonoid glycoside kaempferol 3-O-gentiobioside (KPF 3-O-G) was identified as a potent ALK5 inhibitor through directly bound to the ATP-site of ALK5, resulting in the inhibitory effects on phosphorylation and translocation of Smad2 and expression of Smad4. Additionally, we found that KPF 3-O-G reduced cell proliferation and inhibited TGF-ß-induced cell migration and invasion. Moreover, western blotting and immunofluorescent analysis showed that KPF 3-O-G significantly reversed the TGF-ß-induced EMT biomarkers, including upregulation of E-cadherin and downregulation of N-cadherin, vimentin, and snail. In vivo study showed that KPF 3-O-G administration reduced tumor growth in human ovarian cancer xenograft mouse model, without obvious toxic effect. This study provided novel insight into the anticancer effects of KPF-3-O-G and indicated that KPF-3-O-G might be developed as potential therapeutics for cancer treatment after further validation.


Assuntos
Transição Epitelial-Mesenquimal , Quempferóis , Animais , Linhagem Celular Tumoral , Movimento Celular , Proliferação de Células , Camundongos , Receptor do Fator de Crescimento Transformador beta Tipo I , Transdução de Sinais , Fator de Crescimento Transformador beta
13.
Zhen Ci Yan Jiu ; 46(8): 700-6, 2021 Aug 25.
Artigo em Chinês | MEDLINE | ID: mdl-34472757

RESUMO

Acupuncture treatment can regulate blood pressure (BP) through multiple levels and ways. In the present paper, we reviewed the progress of researches on the underlying mechanisms of acupuncture in lowering BP from 1) regulation of activities of the neuroendocrine, 2) improvement of metabolic abnormality, and 3) alternation of gene expression in the heart and BP-regulation-related centers of the brain. The neuroendocrine mechanism mainly involves the inhibition of neuroinflammatory reaction in some higher brain regions, reduction of neuronal apoptosis, and suppression of the sympathetic cardiovascular regulatory functional areas of the brain stem, regulation of neurotransmitters and autonomic balance, activation of brain areas related to BP regulation, and promotion of functional connection between brain networks. The improvement of metabolic abnormality mainly refers to amelioration of imbalance of intestinal flora and target metabolites related to hypertension. The alteration of gene expression mainly manifests as up- and down-regulation of expression of genes related to oxidative stress, inflammation and vascular endothelial function in the myocardium, hypothalamus, rostral ventrolateral medulla. We reviewed the new research progress on the mechanism of acupuncture for hypertension, in order to provide evidence and research ideas for the treatment of related cardiovascular diseases by using acupuncture therapy in the future.


Assuntos
Terapia por Acupuntura , Hipertensão , Sistema Nervoso Autônomo , Pressão Sanguínea/genética , Humanos , Hipertensão/genética , Hipertensão/terapia , Bulbo
14.
Food Sci Nutr ; 9(9): 4946-4951, 2021 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-34532006

RESUMO

The objective of this work was to investigate the freezing and storage temperature (-80 and -18℃) on the microstructure, protein pattern, and thermal properties of red swamp crayfish after one-week storage, and a Pearson correlation analysis was performed among these attributes. After cryogenic treatments for short-term storage, Tp (pretein denaturation temperature) was significantly raised (p < .05) except for samples frozen at -80℃ prior to store at -18℃ (-80/-18). Samples frozen and stored at -80℃ (-80/-80) had lower number and sum area of white regions in histology, higher intensity of most protein bands in sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) image, and relatively higher Tp and ΔH (p < .05), while -80/-18 samples had lower intensity of most protein bands and TP 2, and higher number and sum area of white regions and ΔH 2 (p < .05). Pearson's analysis results showed the intensive TN T and MLC 1 band could be potentially considered as the markers of tissue integrity and protein degradation. Therefore, the three attributes could be applied to comprehensively assess the quality of frozen aquatic products, and -80/-80 treatment was appropriate for crayfish preservation.

15.
IEEE Trans Image Process ; 30: 8008-8018, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34534081

RESUMO

The problem of generalizing deep neural networks from multiple source domains to a target one is studied under two settings: When unlabeled target data is available, it is a multi-source unsupervised domain adaptation (UDA) problem, otherwise a domain generalization (DG) problem. We propose a unified framework termed domain adaptive ensemble learning (DAEL) to address both problems. A DAEL model is composed of a CNN feature extractor shared across domains and multiple classifier heads each trained to specialize in a particular source domain. Each such classifier is an expert to its own domain but a non-expert to others. DAEL aims to learn these experts collaboratively so that when forming an ensemble, they can leverage complementary information from each other to be more effective for an unseen target domain. To this end, each source domain is used in turn as a pseudo-target-domain with its own expert providing supervisory signal to the ensemble of non-experts learned from the other sources. To deal with unlabeled target data under the UDA setting where real expert does not exist, DAEL uses pseudo labels to supervise the ensemble learning. Extensive experiments on three multi-source UDA datasets and two DG datasets show that DAEL improves the state of the art on both problems, often by significant margins.

16.
Front Med (Lausanne) ; 8: 742419, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34568395

RESUMO

Background: T-lymphocyte subsets reflect patients' immune status and are associated with adverse outcomes in various diseases. However, the association between T-lymphocyte subsets and major infection and renal outcome in chronic kidney disease (CKD) patients has not been well-addressed. Methods: Patients diagnosed with stage 3-5 of non-dialysis CKD were recruited, and healthy subjects were selected as the controls. T-lymphocyte subsets (CD3+, CD4+, CD8+) were detected by flow cytometry, and the CD4+/CD8+ T cell ratio was then calculated. Patients were divided into the normal-level group and the low-level group according to the clinical reference value. The primary outcomes were the major infection and renal outcome. Results: A total of 410 CKD patients were enrolled; the average age was 47.25 years. Compared to the healthy controls, the level of CD3+, CD4+, CD8+ T cells, and the CD4+/CD8+ T cell ratio were significantly decreased in CKD patients (p < 0.05). During the median follow-up of 2.56 (quartile interval 1.24-3.46) years, major infections occurred in 15.10% of the CKD patients. The incidence of infection was significantly higher in the low-level group of CD3+, CD4+ T cells, and CD4+/CD8+ T cell ratio compared with the normal level groups. Kaplan-Meier analysis showed that the lower level of CD3+, CD4+ T cells, and CD4+/CD8+T cell ratio is associated with a greater risk of infection. Cox regression analysis further confirmed that low CD3+, CD4+ T cells, and CD4+/CD8+ T cell ratio were independent risk factors of infection in CKD patients. Moreover, during the follow-up, renal events occurred in 37.50% of patients. Kaplan-Meier analysis indicated that low levels of CD3+, CD4+, and CD8+ T cells are significantly associated with renal outcome in CKD patients. Cox regression analysis showed that low level of CD3+ T cells (HR = 2.407, 95% CI: 1.664-3.482, p < 0.001), CD4+ T cells (HR = 2.397, 95% CI: 1.633-3.518, p < 0.001) and CD8+ T cells (HR = 2.416, 95% CI: 1.476-3.955, p < 0.001) were independent risk factors for renal outcome after multivariable-adjusted. Conclusion: CKD patients had a defect in T-lymphocyte subpopulation. T-lymphocyte subsets were closely associated with infection and renal outcome in CKD patients. Suggesting T-lymphocyte subsets are independent predictors of infection and renal outcome in CKD patients.

17.
IEEE Trans Image Process ; 30: 7914-7925, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34495831

RESUMO

Graph Convolution Network (GCN) has been successfully used for 3D human pose estimation in videos. However, it is often built on the fixed human-joint affinity, according to human skeleton. This may reduce adaptation capacity of GCN to tackle complex spatio-temporal pose variations in videos. To alleviate this problem, we propose a novel Dynamical Graph Network (DG-Net), which can dynamically identify human-joint affinity, and estimate 3D pose by adaptively learning spatial/temporal joint relations from videos. Different from traditional graph convolution, we introduce Dynamical Spatial/Temporal Graph convolution (DSG/DTG) to discover spatial/temporal human-joint affinity for each video exemplar, depending on spatial distance/temporal movement similarity between human joints in this video. Hence, they can effectively understand which joints are spatially closer and/or have consistent motion, for reducing depth ambiguity and/or motion uncertainty when lifting 2D pose to 3D pose. We conduct extensive experiments on three popular benchmarks, e.g., Human3.6M, HumanEva-I, and MPI-INF-3DHP, where DG-Net outperforms a number of recent SOTA approaches with fewer input frames and model size.

18.
Org Lett ; 23(18): 7177-7182, 2021 Sep 17.
Artigo em Inglês | MEDLINE | ID: mdl-34472870

RESUMO

Herein, we disclose a RhIII-catalyzed heteroarylation of C(sp3)-H and C(sp2)-H bonds in heterocycles with organoboron reagents. This protocol displays high efficiency and excellent functional group tolerance. A range of heterocyclic boronates with strong coordinating atoms, including pyridine, pyrimidine, pyrazole, thiophene, and furan derivatives, can be extensively served as the coupling reagents. The direct heteroarylation method could supply potential application in terms of the synthesis of drug molecules with multiple heterocycles.

19.
Zhen Ci Yan Jiu ; 46(9): 782-8, 2021 Sep 25.
Artigo em Chinês | MEDLINE | ID: mdl-34558245

RESUMO

OBJECTIVE: To explore the molecular mechanism of locus coeruleus(LC) involved in electroacupuncture (EA) anti myocardial ischemia. METHODS: Twenty-four SD rats were randomly divided into sham-operation, model, EA and EA +lesion groups, with 6 rats in each group. The acute myocardial ischemia (AMI) model was established by ligation of the left anterior descending branch of coronary artery. EA (2 Hz/15 Hz, 1 mA) was applied to bilateral "Shenmen" (HT7) -"Tongli" (HT5) and the middle-point between HT7 and HT5 for 30 min, once daily for 3 days. For rats of the EA +lesion group, the virus (300 nL) was injected into bilateral LC before EA treatment. Serum aspartate aminotransferase (AST) was detected by ELISA. The gene expression profiles of rat heart were detected by transcriptome sequencing, the differentially expressed genes were screened, and Gene Ontology (GO) functional classification and Kyoto Encyclopedia of genes and genomes (KEGG) metabolic pathway enrichment analysis were performed. RESULTS: Compared with the sham-operation group, serum AST content was significantly increased in the model group (P<0.01). Following the intervention, serum AST was significantly reduced in the EA group (P<0.01), while the serum AST in the EA + lesion group was significantly higher compared with the EA group (P<0.05). Differential expression analysis showed that 1 138 differentially expressed genes were screened out between the model group and the sham-operation group, 1 330 differentially expressed genes between model and EA group, and 804 differentially expressed genes between EA and EA + lesion group. Among them, 218 differential genes were involved in the regulation of EA anti-myocardial ischemia in LC. GO functional classification analysis showed that these differentially expressed genes mainly involved in cell processes, metabolic processes and biological regulation in biological processes. KEGG pathway analysis showed that these differentially expressed genes were enriched in sulfur relay system, thiamine metabolism, glutathione metabolism, C5 branch dicarboxylic acid metabolism, cell adhesion molecules and Th1 and Th2 cell differentiation. CONCLUSION: EA intervention has a positive effect in anti-myocardial ischemia, which may be related to the sulfur relay system, thiamine metabolism, glutathione metabolism, C5 branch dicarboxylic acid metabolism, cell adhesion molecules and Th1 and Th2 cell differentiation involved in LC.


Assuntos
Eletroacupuntura , Isquemia Miocárdica , Pontos de Acupuntura , Animais , Locus Cerúleo , Isquemia Miocárdica/genética , Isquemia Miocárdica/terapia , Ratos , Ratos Sprague-Dawley , Transcriptoma
20.
Chem Commun (Camb) ; 57(78): 10055-10058, 2021 Sep 30.
Artigo em Inglês | MEDLINE | ID: mdl-34505847

RESUMO

Lithium (Li) dendrite growth seriously hinders the practical application of Li metal batteries. Here, we report molecular amidinothiourea (ATU) as a new electrolyte additive to regulate Li stripping/plating behaviors of Li metal anodes. The molecular ATU in the electrolyte can act as a shielding layer on the Li metal surface to suppress the decomposition of electrolytes as verified by XPS and adsorption energy calculation, which improves the electrochemical reversibility of the Li plating/stripping behaviors and inhibits lithium dendrite growth.

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