Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 215
Filtrar
1.
Pathol Res Pract ; 228: 153676, 2021 Oct 30.
Artigo em Inglês | MEDLINE | ID: mdl-34753061

RESUMO

BACKGROUND: Increasing evidences have proved that long noncoding RNAs (lncRNAs) regulate the occurrence of bladder cancer (BC) and participate in various pathophysiology processes. However, little is unknown about the role of lncRNAs in drug resistance of BC cells. In this study, we explored the role of non-coding RNA activated by DNA damage (NORAD) in the gemcitabine (GEM) resistant of BC cells and explored its potential mechanism. METHODS: Real-time quantitative PCR (RT-qPCR) was used to detect the expression of NORAD and miR-155-5p of BC cells. Cell counting kit-8 (CCK-8) and Western blot were used to detect cell inhibition rate and the expression of WEE1 G2 checkpoint kinase (WEE1), P-glycoprotein (P-gp) and multidrug resistance-associated protein 1 (MRP1). Flow cytometry detected cell cycle and apoptosis. Dual luciferase reporter gene assay and RNA immunoprecipitation (RIP) assay were used to confirm the targeting relationship between miR-155-5p, NORAD and WEE1. The xenograft model was used to observe the function of NORAD in vivo. immunohistochemistry (IHC) assay was used to detect the expression of WEE1, caspase-3 and Ki67 in tumor tissues. RESULTS: NORAD highly expressed in GEM-resistant BC cell lines. Knockdown of NORAD significantly inhibited the proliferation of T24/GEM cells, the expression of drug-resistant proteins P-gp and MRP1, inhibit the G0/G1 phase of cells, and induce cell apoptosis. Knockdown of NORAD reversed the promotion effect of miR-155-5p on WEE1 expression and promoted the sensitivity of T24/GEM cells to GEM. In vivo, knockdown of NORAD inhibited the tumor growth, and enhanced the GEM-sensitivity in mice. CONCLUSION: These data highlight the potential of NORAD acts as a therapeutic target for BC GEM resistance. It revealed the vital roles of NORAD/miR-155-5p/WEE1 axis in GEM resistant BC cells, providing a novel therapeutic strategy for BC.

2.
Sci Total Environ ; : 151511, 2021 Nov 08.
Artigo em Inglês | MEDLINE | ID: mdl-34762949

RESUMO

A regional ozone (O3) pollution event occurred in the Yangtze River Delta region during August 17-23, 2020 (except on August 21). This study aims to understand the causes of O3 pollution during the event using an emission-based model (i.e., the Community Multiscale Air Quality (CMAQ) model) and an observation-based model (OBM). The OBM was used to investigate O3 sensitivity to its precursors during the O3 pollution, concluding that O3 formation was limited by volatile organic compounds (VOCs) on August 19, but was co-limited by VOCs and nitrogen oxides (NOx) on other polluted days. Aromatics and alkenes were the two main VOC groups contributing to the O3 formation, with trans-2-butene and m/p-xylene as the key species among the VOCs measured at the Nanjing urban site. The source apportionment results estimated using the source-oriented CMAQ model suggest that the transportation and industry sources dominated the non-background O3 production in Nanjing, which were responsible for 52% and 24.7%, respectively. The O3 concentration attributed to NOx (~70%) was significantly higher than that attributed to VOCs (approximately 30%). The process analysis revealed that vertical mixing increased the O3 concentrations in the early morning, and photochemical reactions promoted O3 formation and accumulation during the daytime within the planetary boundary layer. At night, outflow from horizontal transport and nocturnal chemistry jointly resulted the O3 depletion. The contributions of inter-city transport during the O3 pollution period in Nanjing were also estimated. The predicted O3 concentration was largely recorded from long-distance regions, reaching 46%, followed by local sources (38%) and surrounding cities (16%). The results indicate that both NOx and VOCs contributed significantly to O3 pollution during this event, and the emissions controls of NOx and the key VOC species of aromatics and alkenes from a cooperative regional perspective should be considered to mitigate O3 pollution.

3.
Epigenomics ; 13(19): 1523-1537, 2021 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-34612069

RESUMO

Aims: The genetic association between Behçet's disease susceptibility and IL-10 has been confirmed in multiple cohorts, but the underlying mechanism of this association remains unclear. Materials & methods: We combined public resources and laboratory experiments (electrophoretic mobility shift assays, chromatin immunoprecipitation, luciferase reporter gene and CRISPR/Cas9 genome editing) to analyze transcription factor binding and enhancer activity controlling IL-10 expression. Results & conclusion: The T allele of noncoding rs3024490 within super-enhancer elements is able to specifically bind TBX1 and, in turn, promotes the enhancer activity and increased expression of IL-10. However, a relative deficiency in TBX1 in Behçet's disease patients leads to the low expression of IL-10 and increased risk of developing Behçet's disease.

4.
Soft Matter ; 17(40): 9125-9130, 2021 Oct 20.
Artigo em Inglês | MEDLINE | ID: mdl-34570151

RESUMO

Utilizing combined non-covalent interactions and introducing anions as structure-directing factors to build oriented self-assembly and 2D crystalline nanosheet superstructures with precise distance control of surface charges in competitive aqueous solvents still represents a formidable challenge for supramolecular chemists. Here we report a simple, efficient, and general strategy for multiple C-H/N-H⋯anion hydrogen bond enhanced π-π interaction directed 2D oriented self-assembly in water, which is based on the head-to-tail association of perylene monoimide dimers (PMIs) by directing N-H⋯anion interactions to position the anions to the C-H of π systems (PMIs). Interesting, this behavior only occurs for size-matched anions (Cl- to NO3-; <45 Å3), while larger anions could not form 2D crystalline nanosheet superstructures. The results show that crystalline nanosheet superstructures with precise distance control of surface charges can effectively capture DNA, possibly due to their high surface charge density and the distance match between the distance of surface charges and the distance between adjacent base pairs.


Assuntos
DNA , Ânions , Pareamento de Bases , Ligação de Hidrogênio
5.
Int J Mol Sci ; 22(18)2021 Sep 20.
Artigo em Inglês | MEDLINE | ID: mdl-34576293

RESUMO

Photodynamic therapy (PDT) is a clinical treatment for cancer or non-neoplastic diseases, and the photosensitizers (PSs) are crucial for PDT efficiency. The commonly used chemical PSs, generally produce ROS through the type II reaction that highly relies on the local oxygen concentration. However, the hypoxic tumor microenvironment and unavoidable dark toxicity of PSs greatly restrain the wide application of PDT. The genetically encoded PSs, unlike chemical PSs, can be modified using genetic engineering techniques and targeted to unique cellular compartments, even within a single cell. KillerRed, as a dimeric red fluorescent protein, can be activated by visible light or upconversion luminescence to execute the Type I reaction of PDT, which does not need too much oxygen and surely attract the researchers' focus. In particular, nanotechnology provides new opportunities for various modifications of KillerRed and versatile delivery strategies. This review more comprehensively outlines the applications of KillerRed, highlighting the fascinating features of KillerRed genes and proteins in the photodynamic systems. Furthermore, the advantages and defects of KillerRed are also discussed, either alone or in combination with other therapies. These overviews may facilitate understanding KillerRed progress in PDT and suggest some emerging potentials to circumvent challenges to improve the efficiency and accuracy of PDT.


Assuntos
Proteínas de Fluorescência Verde/metabolismo , Fotoquimioterapia/métodos , Fármacos Fotossensibilizantes/metabolismo , Animais , Proteínas de Fluorescência Verde/genética , Proteínas de Fluorescência Verde/uso terapêutico , Humanos , Fármacos Fotossensibilizantes/uso terapêutico , Engenharia de Proteínas/métodos
6.
Artigo em Inglês | MEDLINE | ID: mdl-34524450

RESUMO

OBJECTIVE: There have been various methods to deal with the erroneous training data in distantly supervised relation extraction (RE), however, their performance is still far from satisfaction. We aimed to deal with the insufficient modeling problem on instance-label correlations for predicting biomedical relations using deep learning and reinforcement learning. MATERIALS AND METHODS: In this study, a new computational model called piecewise attentive convolutional neural network and reinforcement learning (PACNN+RL) was proposed to perform RE on distantly supervised data generated from Unified Medical Language System with MEDLINE abstracts and benchmark datasets. In PACNN+RL, PACNN was introduced to encode semantic information of biomedical text, and the RL method with memory backtracking mechanism was leveraged to alleviate the erroneous data issue. Extensive experiments were conducted on 4 biomedical RE tasks. RESULTS: The proposed PACNN+RL model achieved competitive performance on 8 biomedical corpora, outperforming most baseline systems. Specifically, PACNN+RL outperformed all baseline methods with the F1-score of 0.5592 on the may-prevent dataset, 0.6666 on the may-treat dataset, and 0.3838 on the DDI corpus, 2011. For the protein-protein interaction RE task, we obtained new state-of-the-art performance on 4 out of 5 benchmark datasets. CONCLUSIONS: The performance on many distantly supervised biomedical RE tasks was substantially improved, primarily owing to the denoising effect of the proposed model. It is anticipated that PACNN+RL will become a useful tool for large-scale RE and other downstream tasks to facilitate biomedical knowledge acquisition. We also made the demonstration program and source code publicly available at http://112.74.48.115:9000/.

8.
J Agric Food Chem ; 69(37): 11006-11014, 2021 Sep 22.
Artigo em Inglês | MEDLINE | ID: mdl-34491745

RESUMO

Many phytochemicals suffer from poor water dispersity and storage stability, which restrict their application within aqueous-based commercial products. ß-Cyclodextrin (ß-CD) is a water-dispersible molecule with a hydrophobic core that can encapsulate and protect non-polar substances. The functional attributes of ß-CD can be further enhanced by chemical modification. In this study, a simple and effective dry-heating process was applied to fabricate succinic acid (SA)-modified ß-CD (SACD) through esterification. SACD showed better encapsulation property than non-modified ß-CD to guest molecules such as methyl orange (up to 1.41-folds of ß-CD) and curcumin (with an encapsulation efficiency of up to 10 mg/g). Meanwhile, higher water solubility (up to 469.30 g per 100 g of H2O) was achieved for SACD, indicating that a high dose of SACD could be applied in an aqueous food matrix. Such a simple strategy exhibiting low cytotoxicity shows great potential incorporating bioactive compounds into functional foods.


Assuntos
Curcumina , Ciclodextrinas , Interações Hidrofóbicas e Hidrofílicas , Solubilidade , Água
9.
Preprint em Inglês | bioRxiv | ID: ppbiorxiv-460185

RESUMO

Coronavirus disease 2019 (COVID-19) caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is currently the most significant public health threats in worldwide. Patients with severe COVID-19 usually have pneumonia concomitant with local inflammation and sometimes a cytokine storm. Specific components of the SARS-CoV-2 virus trigger lung inflammation, and recruitment of immune cells to the lungs exacerbates this process, although much remains unknown about the pathogenesis of COVID-19. Our study of lung type II pneumocyte cells (A549) demonstrated that ORF7, an open reading frame (ORF) in the genome of SARS-CoV-2, induced the production of CCL2, a chemokine that promotes the chemotaxis of monocytes, and decreased the expression of IL-8, a chemokine that recruits neutrophils. A549 cells also had an increased level of IL-6. The results of our chemotaxis transwell assay suggested that ORF7 augmented monocyte infiltration and reduced the number of neutrophils. We conclude that the ORF7 of SARS-CoV-2 may have specific effects on the immunological changes in tissues after infection. These results suggest that the functions of other ORFs of SARS-CoV-2 should also be comprehensively examined.

10.
Huan Jing Ke Xue ; 42(9): 4116-4125, 2021 Sep 08.
Artigo em Chinês | MEDLINE | ID: mdl-34414710

RESUMO

Organic carbon (OC), elemental carbon (EC), and PM2.5 concentration data obtained from Shanxi Super Station in Jiashan County of Jiaxing City, in the winter of 2018 and 2019, were analyzed to determine the variation and potential source areas of carbonaceous aerosols. The results show that OC concentrations in the winter of 2018 and 2019 were 6.90 µg·m-3 and 5.63 µg·m-3, respectively, while EC concentrations were 2.47 µg·m-3 and 1.57 µg·m-3, respectively. The concentrations of OC and EC in the winter of 2019 were lower than those in the winter of 2018, by approximately 18.4% and 36.4%, respectively. In 2018 and 2019, the concentrations of secondary organic carbon (SOC), calculated using the minimum R-squared (MRS) method, were 1.49 µg·m-3 and 1.97 µg·m-3, respectively, and the concentrations of primary organic carbon (POC) were 5.41 µg·m-3 and 3.66 µg·m-3, respectively. The proportion of POC in OC showed a downward trend, from 96.0% in December 2018 to 64.9% in February 2020, indicating a decrease of 31.1 percentage points. SOC showed an upward trend, increasing by 31.1 percentage points from 4.0% in December 2018 to 35.1% in February 2020. It is worth noting that with the increase in PM2.5 concentration, the concentration of OC and EC increased by 474.7% and 408.2%, respectively, although the proportion of OC in PM2.5 decreased from 18.8% to 12.3%. and the percentage of OC decreased from 5.8% to 3.3%. The contribution of POC to PM2.5 did not fluctuate, and only decreased significantly above 150 µg·m-3, while the contribution of SOC to PM2.5 first decreased and then increased. In Jiaxing, the potential sources of OC and EC were mainly southern Jiangsu, southeastern Anhui, local Jiaxing, and northern Zhejiang. In the winter of the contribution concentrations of OC and EC in the main potential source regions were approximately 2 µg·m-3 and 6 µg·m-3 lower, respectively, than in winter 2018. The range of high values in the potential source regions also decreased in 2019. Before the COVID-19 epidemic, it was affected by both motor vehicle exhaust emissions and coal burning. During the Spring Festival and home isolation, due to traffic control and other reasons, motor vehicle emissions were reduced, which leaving coal burning as the main contributor.


Assuntos
Poluentes Atmosféricos , COVID-19 , Aerossóis/análise , Poluentes Atmosféricos/análise , Monitoramento Ambiental , Humanos , Material Particulado/análise , SARS-CoV-2
11.
Front Oncol ; 11: 686044, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34422642

RESUMO

Background: Bladder cancer (BC) is a molecular heterogeneous malignant tumor; the treatment strategies for advanced-stage patients were limited. Therefore, it is vital for improving the clinical outcome of BC patients to identify key biomarkers affecting prognosis. Ferroptosis is a newly discovered programmed cell death and plays a crucial role in the occurrence and progression of tumors. Ferroptosis-related genes (FRGs) can be promising candidate biomarkers in BC. The objective of our study was to construct a prognostic model to improve the prognosis prediction of BC. Methods: The mRNA expression profiles and corresponding clinical data of bladder urothelial carcinoma (BLCA) patients were downloaded from The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) databases. FRGs were identified by downloading data from FerrDb. Differential analysis was performed to identify differentially expressed genes (DEGs) related to ferroptosis. Univariate and multivariate Cox regression analyses were conducted to establish a prognostic model in the TCGA cohort. BLCA patients from the GEO cohort were used for validation. Gene ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), and single-sample gene set enrichment analysis (ssGSEA) were used to explore underlying mechanisms. Results: Nine genes (ALB, BID, FADS2, FANCD2, IFNG, MIOX, PLIN4, SCD, and SLC2A3) were identified to construct a prognostic model. Patients were classified into high-risk and low-risk groups according to the signature-based risk score. Receiver operating characteristic (ROC) and Kaplan-Meier (K-M) survival analysis confirmed the superior predictive performance of the novel survival model based on the nine-FRG signature. Multivariate Cox regression analyses showed that risk score was an independent risk factor associated with overall survival (OS). GO and KEGG enrichment analysis indicated that apart from ferroptosis-related pathways, immune-related pathways were significantly enriched. ssGSEA analysis indicated that the immune status was different between the two risk groups. Conclusion: The results of our study indicated that a novel prognostic model based on the nine-FRG signature can be used for prognostic prediction in BC patients. FRGs are potential prognostic biomarkers and therapeutic targets.

12.
Front Endocrinol (Lausanne) ; 12: 704620, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34335475

RESUMO

Hydrogen sulfide (H2S), as one of the three known gaseous signal transduction molecules in organisms, has attracted a surging amount of attention. H2S is involved in a variety of physiological and pathological processes in the body, such as dilating blood vessels (regulating blood pressure), protecting tissue from ischemia-reperfusion injury, anti-inflammation, carcinogenesis, or inhibition of cancer, as well as acting on the hypothalamus and pancreas to regulate hormonal metabolism. The change of H2S concentration is related to a variety of endocrine disorders, and the change of hormone concentration also affects the synthesis of H2S. Understanding the effect of biosynthesis and the concentration of H2S on the endocrine system is useful to develop drugs for the treatment of hypertension, diabetes, and other diseases.

13.
Exp Ther Med ; 22(3): 962, 2021 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-34335904

RESUMO

The Chinese decoction compound centella formula (CCF) is clinically effective against diabetic kidney disease (DKD), but the exact mechanism remains unclear. The present study aimed to investigate the effects of CCF on OxInflammation and silent information regulator 1 (SIRT1) levels in rats with streptozotocin (STZ)-induced diabetes. Sprague-Dawley rats were divided into CCF, losartan, diabetic control (DC) and normal control (NC) groups (n=7). Except for the NC, all subgroups of rats were fed a high-fat diet for 112 days and received a single intraperitoneal injection of 35 mg/kg STZ on day 29. All rats were sacrificed on day 112. High-performance liquid chromatography was performed to analyse asiaticoside, astragaloside and triptolide levels in CCF (0.3400, 0.0640 and 0.0001 mg/ml, respectively). Fasting blood glucose, urine protein-to-creatinine ratio, serum creatinine and blood urea nitrogen were quantified. Periodic acid Schiff staining, H&E staining and transmission electron microscopy were used to examine kidney pathological changes. The mRNA and protein expression levels of SIRT1 in renal tissues were analysed by reverse transcription-quantitative PCR, western blotting and immunohistochemistry. Oxidative stress was evaluated by measuring the levels of superoxide dismutase (SOD), malondialdehyde (MDA) and nicotinamide adenine dinucleotide phosphate oxidase 4 (NOX4) in renal tissues. TNF-α and NF-κB p65 subunit in renal tissues were assessed for inflammation. Compared with the rats in the NC group, the rats in the DC group exhibited renal injury with proteinuria, decreased expression levels of SIRT1 and SOD (P<0.01) and increased levels of MDA, NOX4, TNF-α and NF-κB p65 (P<0.01). CCF treatment reduced proteinuria (P<0.01), alleviated renal damage, decreased MDA, NOX4, TNF-α and NF-κB p65 levels (P<0.01), increased SOD levels (P<0.05) and increased SIRT1 mRNA and protein expression levels (P<0.01). The present study indicates that CCF effectively protects the kidney from diabetes by inhibiting OxInflammation and upregulating SIRT1.

14.
Langmuir ; 37(28): 8486-8497, 2021 07 20.
Artigo em Inglês | MEDLINE | ID: mdl-34236199

RESUMO

Recently, a burgeoning category of biocompatible botanically derived nanomaterial cellulose nanofibers (CNFs) has captured tremendous attention on account of its entangled nanostructured network, natural abundance, and outstanding mechanical properties. Biomimetically inspired by the superior properties of CNFs, this paper examined them as the coating material to cover cyclotrimethylenetrinitramine (RDX), cyclotetramethylenetetranitramine (HMX), and hexanitrohexaazaisowurtzitane (CL-20) via a facile water suspension method and the ultrasonic technology. The core-shell structure and the composition of energetic crystal@CNF were examined through scanning electron microscopy, X-ray diffraction, Fourier transform infrared spectroscopy, Raman spectroscopy, and X-ray photoelectron spectroscopy analyses. The obtained outcomes demonstrated that the dispersibility of the CNF enhanced favorably upon covering the surface of explosive crystals; the interfacial contact ability between CNFs and energetic crystals was also manifested to be increased, which could be ascribed to the interfacial interaction of hydrogen bonds and the electrostatic force of self-assembly. In addition, the stable crystalloid construction of ß-HMX and ε-CL-20 has been preserved positively in the preparation process. In comparison with raw explosives, the thermal stability and sensitivity performances of the core-shell structure composites were outstanding. Accordingly, this work demonstrated the rewarding application of coating CNFs uniformly on the surface of energetic crystals, ulteriorly offering a potential fabrication strategy for the embellishment of high-explosive crystals.


Assuntos
Substâncias Explosivas , Nanofibras , Compostos de Anilina , Celulose , Nitrobenzenos
15.
Sci Total Environ ; 792: 148349, 2021 Oct 20.
Artigo em Inglês | MEDLINE | ID: mdl-34147813

RESUMO

We investigated the spatial distribution and trend of double high pollution (DHP), in which the daily average concentration of fine particulate matter (PM2.5) was above 75 µg/m3 and the daily maximum 8-hour average ozone (MDA8 O3) concentration was above 160 µg/m3, in the Yangtze River Delta (YRD) region during 2015-2019, along with the meteorological and chemical characteristics during DHP and differences compared to high O3 pollution (HOP) and high PM2.5 pollution (HPP). In the YRD, Shanghai had the highest frequency of DHP at 7.6%, while Anhui had the least (2.1%). DHP mostly occurred in the northwest and along the Yangtze River in the east of the YRD, especially in spring (April) and autumn (October). MDA8 O3 level was relatively higher during DHP than HOP, while PM2.5 level was relatively higher during HPP than DHP. In 2015-2019, the total number of DHP events decreased in the YRD, but the changes in PM2.5 and O3 concentrations showed great spatial variations. DHP was often associated with a weak pressure field, under meteorological conditions with east winds, temperatures of 18.7-26.1 °C, relative humidity of 65.7-77.1%, sea level pressure of 1008.2-1019 hPa, wind speed of 1.4-2.4 m/s, and visibility of 3.1-7.5 km. Water-soluble ions (NO3-, NH4+, and SO42-) were the dominant components of PM2.5 during DHP at Nanjing and Changzhou City in 2019. Although the fraction of those ions during DHP and HPP were similar, the secondary conversion of NO2 and SO2 was stronger in HPP. The concentrations of those ions were lowest in HOP, with a higher fraction of sulfate than the other two types of pollution. The conversion of SO2 to sulfate was easier to occur than that of NO2 to nitrate under all the polluted conditions in the two cities.


Assuntos
Poluentes Atmosféricos , Poluição do Ar , Poluentes Atmosféricos/análise , Poluição do Ar/análise , China , Monitoramento Ambiental , Material Particulado/análise , Rios , Estações do Ano
16.
Genome Biol ; 22(1): 175, 2021 06 09.
Artigo em Inglês | MEDLINE | ID: mdl-34108023

RESUMO

BACKGROUND: The maize inbred line A188 is an attractive model for elucidation of gene function and improvement due to its high embryogenic capacity and many contrasting traits to the first maize reference genome, B73, and other elite lines. The lack of a genome assembly of A188 limits its use as a model for functional studies. RESULTS: Here, we present a chromosome-level genome assembly of A188 using long reads and optical maps. Comparison of A188 with B73 using both whole-genome alignments and read depths from sequencing reads identify approximately 1.1 Gb of syntenic sequences as well as extensive structural variation, including a 1.8-Mb duplication containing the Gametophyte factor1 locus for unilateral cross-incompatibility, and six inversions of 0.7 Mb or greater. Increased copy number of carotenoid cleavage dioxygenase 1 (ccd1) in A188 is associated with elevated expression during seed development. High ccd1 expression in seeds together with low expression of yellow endosperm 1 (y1) reduces carotenoid accumulation, accounting for the white seed phenotype of A188. Furthermore, transcriptome and epigenome analyses reveal enhanced expression of defense pathways and altered DNA methylation patterns of the embryonic callus. CONCLUSIONS: The A188 genome assembly provides a high-resolution sequence for a complex genome species and a foundational resource for analyses of genome variation and gene function in maize. The genome, in comparison to B73, contains extensive intra-species structural variations and other genetic differences. Expression and network analyses identify discrete profiles for embryonic callus and other tissues.

17.
J Transcult Nurs ; 32(6): 778-789, 2021 11.
Artigo em Inglês | MEDLINE | ID: mdl-34120529

RESUMO

INTRODUCTION: Nursing education is challenged to improve students' cultural competence. Clinical simulation using standardized patients (SPs) may be an effective learning intervention. This systematic review was to identify current evidence on the use of simulations with SPs as learning interventions that have been developed to improve nursing students' cultural competence and on the effectiveness of those interventions. METHOD: Using published guidelines, we conducted a systematic review of studies on simulation using SPs to teach cultural competence. RESULTS: Ten studies met the inclusion criteria. Outcomes of simulation with SPs increased levels of nursing students' cultural competence. Simulations with SPs were used in theoretical and practicum courses of nursing students' cultural competence, often combined with case-study and video presentations. Other forms of cultural education also improved cultural competence. DISCUSSION: This study supports that a combination of lecture, case-based learning, and simulation with SPs can increase nursing students' cultural competence.

18.
Chemosphere ; 278: 130483, 2021 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-34126692

RESUMO

Rapid industrialization and urbanization have produced a lot of hazardous substances in water and wastewater, which has turned into a crucial issue to the environment and the public health. Recently, iron carbon microelectrolysis (IC-ME) has attracted extensive attention in environmental remediation due to its low costs and excellent performance. Nevertheless, there is still a lack of a more systematic review on IC-ME preparation methods, their performance, and the interaction mechanisms of IC-ME in the remediation of wastewater. Herein, this work summarizes the synthetic methods, application of IC-ME materials, and the mechanism of pollutant removal by IC-ME. A variety approaches have been applied to prepare IC-ME materials, and the preparation methods and conditions have a certain influence on the properties of IC-ME materials, thus affecting the performance of pollutant removal. The mechanisms of IC-ME for contaminants removal are very complex, including adsorption, coprecipitation, reduction, surface complexation, and oxidation. Moreover, research vacant fields and problems that existed in the application of IC-ME are proposed. At last, the problems to be addressed to adapt IC to future applications are introduced. This paper reviews and prospects IC-ME wastewater remediation technology, which provides a reference for further scientific research and engineering applications.


Assuntos
Recuperação e Remediação Ambiental , Poluentes Químicos da Água , Adsorção , Carbono , Ferro , Águas Residuárias , Poluentes Químicos da Água/análise
19.
J Environ Sci (China) ; 104: 150-168, 2021 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-33985718

RESUMO

Using a bottom-up estimation method, a comprehensive, high-resolution emission inventory of gaseous and particulate atmospheric pollutants for multiple anthropogenic sectors with typical local sources has been developed for the Harbin-Changchun city agglomeration (HCA). The annual emissions for CO, NOx, SO2, NH3, VOCS, PM2.5, PM10, BC and OC during 2017 in the HCA were estimated to be 5.82 Tg, 0.70 Tg, 0.34 Tg, 0.75 Tg, 0.81Tg, 0.67 Tg, 1.59 Tg, 0.12 Tg and 0.26 Tg, respectively. For PM10 and SO2, the emissions from industry processes were the dominant contributors representing 54.7% and 49.5%, respectively, of the total emissions, while 95.3% and 44.5% of the total NH3 and NOx emissions, respectively, were from or associated with agricultural activities and transportation. Spatiotemporal distributions showed that most emissions (except NH3) occurred in November to March and were concentrated in the central cities of Changchun and Harbin and the surrounding cities. Open burning of straw made an important contribution to PM2.5 in the central regions of the northeastern plain during autumn and spring, while domestic coal combustion for heating purposes was significant with respect to SO2 and PM2.5 emissions during autumn and winter. Furthermore, based on Principal Component Analysis and Multivariable Linear Regression model, air temperature, relative humidity, electricity and energy consumption, and the urban and rural population were optimized to be representative indicators for rapidly assessing the magnitude of regional atmospheric pollutants in the HCA. Such indicators and equations were demonstrated to be useful for local atmospheric environment management.


Assuntos
Poluentes Atmosféricos , Poluição do Ar , Poluentes Ambientais , Poluentes Atmosféricos/análise , Poluição do Ar/análise , China , Cidades , Conservação dos Recursos Naturais , Monitoramento Ambiental , Humanos , Material Particulado/análise
20.
Sichuan Da Xue Xue Bao Yi Xue Ban ; 52(3): 430-437, 2021 May.
Artigo em Chinês | MEDLINE | ID: mdl-34018361

RESUMO

Objective: To investigate the effect of E74-like factor 5 (ELF5) overexpression on the growth and invasion ability of colorectal cancer cells and its effect on tumor formation in nude mice. Methods: Human colorectal cancer SW480 and HT-29 cells were divided into 5 groups: the lentivirus (LV)- GFP group transfected with empty vector LV- GFP, the LV- ELF5 group transfected with recombinant LV- ELF5, the shRNA-NC group transfected with empty vector shRNA-NC, the shRNA- ELF5 group transfected with recombinant shRNA- ELF5, and the control group, not transfected with any vector. Seventy-two h after transfection, the cell supernatant containing lentivirus was collected. The mRNA expression level of ELF5 in each group was examined by real-time fluorescent quantitative PCR (RT-qPCR). The protein expression levels of ELF5, apoptosis-related cleaved Caspase-3/Caspase-3 and cleaved Caspase-9/Caspase-9, and invasion-related E-cadherin and N-cadherin were checked with Western blot. CCK-8 was used to check cell viability. Colony formation experiment was done to evaluate colony formation rate. Flow cytometry was used to assess cell apoptosis. Transwell migration assay was used to examine cell invasion. TUNEL assay was used to examine the apoptosis of tissues cells. Immunohistochemistry test was done to determine the expression of E-cadherin and N-cadherin in tissues. 20 BALB/c nude mice were put into 4 groups (5 in each group): LV- GFP group, shRNA-NC group, LV- ELF5 group, and shRNA- ELF5 group. Recombinant lentiviral SW480 cell supernatants were subcutaneously injected into nude mice to construct nude mice tumorigenesis models and the volume changes of transplanted tumors were monitored. On the 30th day, transplanted tumor tissues from the nude mice were extracted and the tumor mass was measured. Western blot was done to measure the expression of ELF4 protein in the transplanted tumors. TUNEL staining was used to check cell apoptosis in the tissues, and the positive expression of N-cadherin in the transplanted tumor was measured by immunohistochemical tests. Results: Compared with the control group, there was no statistically significant difference in the indicators of the two cell lines in the LV- GFP group and shRNA-NC group. The results of Western blot and RT-qPCR showed that the ELF5 protein and mRNA of the LV- ELF5 group of the two cell lines were up-regulated ( P<0.05, compared with those of the LV- GFP group), and the ELF5 protein and mRNA of the shRNA- ELF5 group were down-regulated ( P<0.05). The ELF5 overexpression system and interference system were successfully constructed. Compared with the LV- GFP group, data from the LV- ELF5 group showed that cell viability and colony formation rate ( P<0.05) were reduced, SW480 and HT-29 cell apoptosis was promoted, cleaved Caspase-3/Caspase-3 and cleaved Caspase-9/Caspase-9 protein expression was up-regulated ( P<0.05), cell invasion was inhibited, and the expression of E-cadherin protein was up-regulated while the expression of N-cadherin protein was down-regulated ( P<0.05). After ELF5 interference, the above-mentioned expression of cells demonstrated an opposite trend ( P<0.05, comparing shRNA- ELF5 group with shRNA-NC group). In vivo experimental results indicated that ELF5 overexpression reduced tumor volume and tumor mass ( P<0.05), promoted cell apoptosis in tissues ( P<0.05), and inhibited N-cadherin protein expression ( P<0.05). When ELF5 expression was inhibited, the above mentioned experimental results showed the opposite trend. Conclusion: In vivo and in vitro experiments showed that ELF5 overexpression could promote the apoptosis of colorectal cancer cells and inhibit the growth and invasion of colorectal cancer cells.


Assuntos
Neoplasias do Colo , Fator V , Animais , Apoptose/genética , Linhagem Celular Tumoral , Proliferação de Células , Neoplasias do Colo/genética , Proteínas de Ligação a DNA , Camundongos , Camundongos Endogâmicos BALB C , Camundongos Nus , Fatores de Transcrição , Transfecção
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...