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1.
Asian J Androl ; 2021 Oct 29.
Artigo em Inglês | MEDLINE | ID: mdl-34747721

RESUMO

This paper presents a meta-analysis regarding the detection rate (DR) of fluorine-18 (18F)-labeled prostate-specific membrane antigen positron emission tomography/computed tomography (PSMA PET/CT) in the management of patients with prostate cancer (PCa). Relevant studies regarding 18F-PSMA PET/CT in the management of PCa published until June 1, 2021, were electronically searched in online databases including EMBASE, PubMed, and Web of Science. The primary outcome was the DR of 18F-PSMA PET/CT in managing PCa patients, while the secondary outcome was the DR of 18F-PSMA PET/CT according to Gleason scores and serum prostate-specific antigen (PSA) level. The pooled DR was calculated on a per-patient basis, with pooled odd ratios and 95% confidence intervals (CIs). In total, 17 observational studies evaluating 1019 patients with PCa met the inclusion criteria. The DR of 18F-PSMA PET/CT was 0.83 (95% CI: 0.78-0.88), in the random-effects model. Subsequently, the analysis of DR of 18F-PSMA PET/CT in PCa patients using Gleason score (≤7 vs ≥8), showed a significant difference in PCa patients. Based on the above results, the higher Gleason score of PCa patients, the higher DR of 18F-PSMA PET/CT. The DR of 18F-PSMA PET/CT in PCa was 0.57 for PSA <0.5 ng ml-1; 0.75 for PSA ≥0.5 ng ml-1 and <1.0 ng ml-1; 0.93 for PSA ≥1.0 ng ml-1 and <2.0 ng ml-1; and 0.95 for PSA ≥2.0 ng ml-1. Therefore, the significant diagnostic value was found in terms of the DR of 18F-PSMA PET/CT in managing PCa patients and was associated with Gleason score and serum PSA level.

2.
Int J Infect Dis ; 112: 152-164, 2021 Aug 30.
Artigo em Inglês | MEDLINE | ID: mdl-34474147

RESUMO

BACKGROUND: Schistosomiasis is a parasitic disease caused by hematodes of genus Schistosoma. This review evaluated the available nucleic acid amplification techniques for diagnosing S. mansoni infections in humans, intermediate host snails, and presumed rodent reservoirs. METHODS: Sensitivity, specificity, diagnostic odds ratio (DOR), and 95% CI were calculated based on available literature. The potential of PCR, nPCR, PCR-ELISA, qPCR, and LAMP was compared for diagnosing S. mansoni infections. RESULTS: A total of 546 published records were identified. Quality assessment by QUADAS-2 revealed an uncertain risk in most studies, and 21 references were included in the final. For human samples, the four nucleic acid amplification techniques showed an overall sensitivity of 89.79% (95% CI: 83.92%-93.67%), specificity of 87.70% (95% CI: 72.60%-95.05%), and DOR of 37.73 (95% CI: 21.79-65.33). LAMP showed the highest sensitivity, followed by PCR-ELISA, PCR, and qPCR, while this order was almost reversed for specificity; qPCR had the highest AUC. For rodent samples, qPCR showed modest sensitivity (68.75%, 95% CI: 43.32%-86.36%) and high specificity (92.45%, 95% CI: 19.94%-99.83%). For snail samples, PCR and nPCR assays showed high sensitivity of 90.06% (95% CI: 84.39%-93.82%) and specificity of 85.51% (95% CI: 54.39%-96.69%). CONCLUSION: Nucleic acid amplification techniques had high diagnostic potential for identifying S. mansoni infections in humans.

3.
Parasit Vectors ; 13(1): 273, 2020 Jun 01.
Artigo em Inglês | MEDLINE | ID: mdl-32487266

RESUMO

BACKGROUND: Fishermen and boatmen are a population at-risk for contracting schistosomiasis due to their high frequency of water contact in endemic areas of schistosomiasis in the People's Republic of China (P. R. China). To develop specific interventions towards this population, the present study was designed to assess the knowledge, attitudes and practices (KAPs) towards schistosomiasis of fishermen and boatmen, and to identify the risk factors associated with schistosome infection using a molecular technique in a selected area of Hunan Province in P. R. China. METHODS: A cross sectional survey was conducted in the Dongting Lake Basin of Yueyang County, Hunan Province. A total of 601 fishermen and boatmen were interviewed between October and November 2017. Information regarding sociodemographic details and KAPs towards schistosomiasis were collected using a standardized questionnaire. Fecal samples of participants were collected and tested by polymerase chain reaction (PCR). Logistic regression analysis was conducted to explore the risk factors related to the positive results of PCR. RESULTS: Of the 601 respondents, over 90% knew schistosomiasis and how the disease was contracted, the intermediate host of schistosomes and preventive methods. The majority of respondents had a positive attitude towards schistosomiasis prevention. However, only 6.66% (40/601) of respondents had installed a latrine on their boats, while 32.61% (196/601) of respondents defecated in the public toilets on shore. In addition, only 4.99% (30/601) respondents protected themselves while exposed to freshwater. The prevalence of schistosomiasis, as determined by PCR, among fishermen and boatmen in Yueyang County was 13.81% (83/601). Age, years of performing the current job, number of times receiving treatment, and whether they were treated in past three years were the main influencing factors of PCR results among this population. CONCLUSIONS: Fishermen and boatmen are still at high risk of infection in P. R. China and gaps exist in KAPs towards schistosomiasis in this population group. Chemotherapy, and health education encouraging behavior change in combination with other integrated approaches to decrease the transmission risk in environments should be improved.


Assuntos
Pesqueiros , Conhecimentos, Atitudes e Prática em Saúde , Esquistossomose/epidemiologia , Adulto , Animais , China/epidemiologia , Estudos Transversais , Fezes/parasitologia , Feminino , Educação em Saúde , Humanos , Lagos , Masculino , Pessoa de Meia-Idade , Ocupações/estatística & dados numéricos , Prevalência , Fatores de Risco , Esquistossomose/diagnóstico , Esquistossomose/parasitologia , Navios , Inquéritos e Questionários
4.
Adv Parasitol ; 110: 1-62, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32563322

RESUMO

Being a zoonotic parasitic disease, schistosomiasis was widely spread in 12 provinces of Southern China in the 1950s, severly harming human health and hindering economic development. The National Institute of Parasitic Diseases at the Chinese Center for Diseases Control and Prevention, and Chinese Center for Tropical Diseases Research (NIPD-CTDR), as the only professional institution focussing on parasitic diseases at the national level, has played an important role in schistosomiasis control in the country. In this article, we look back at the changes of schistosomiasis endemicity and the contribution of NIPD-CTDR to the national schistosomiasis control programme. We review NIPD-CTDR's activities, including field investigations, design of control strategies and measures, development of diagnostics and drugs, surveillance-response of endemic situation, and monitoring & evaluation of the programme. The NIPD-CTDR has mastered the transmission status of schistosomiasis, mapped the snail distribution, and explored strategies and measures suitable for different types of endemic areas in China. With a good understanding of the life cycle of Schistosoma japonicum and transmission patterns of the disease, advanced research carried out in the NIPD-CTDR based on genomics and modern technology has made it possible to explore highly efficient and soft therapeutic drugs and molluscicides, making it possible to develop new diagnostic tools and produce vaccine candidates. In the field, epidemiological studies, updated strategies and targeted intervention measures developed by scientists from the NIPD-CTDR have contributed significantly to the national schistosomiasis control programme. This all adds up to a strong foundation for eliminating schistosomiasis in China in the near future, and recommendations have been put forward how to reach this goal.


Assuntos
Academias e Institutos , Doenças Endêmicas/prevenção & controle , Programas Governamentais , Programas Nacionais de Saúde , Esquistossomose Japônica , Animais , Bovinos , China/epidemiologia , Erradicação de Doenças , Desenvolvimento de Medicamentos , Humanos , Moluscocidas , Esquistossomose Japônica/tratamento farmacológico , Esquistossomose Japônica/epidemiologia , Esquistossomose Japônica/transmissão , Vacinação
5.
Urol J ; 16(3): 224-231, 2019 06 17.
Artigo em Inglês | MEDLINE | ID: mdl-31004338

RESUMO

PURPOSE: Tamsulosin, a medical expulsive therapy (MET), was always recommended for patients with distal ure-teral calculi less than 10 mm. The aim of the systematic review was to assess the efficacy and safety of tamsulosin in MET compared with placebo. MATERIALS AND METHODS: A comprehensive search was conducted in the databases PubMed, EMBASE and Web of Science for relevant articles, covering all the literatures published until April 2018. All placebo controlled trails were identified in which patients were randomized to receive either tamsulosin or placebo for distal ureteral calculi. RESULTS: A total of seven placebo controlled studies including 4135 patients met the inclusion criteria and were involved in the review. We found that tamsulosin was associated with a significantly higher expulsion rate (ESR) [odds ratio (OR) = 1.10, 95% confidence interval (CI) = 1.00-1.21] than placebo in patients with distal ureteral stones less than 7 mm. The ESR ranged from 67.0%-90.7%. But the significant difference was better seen in pa-tients with distal ureteral stones less than 10 mm (OR = 1.11, 95% CI = 1.01-1.21). Even though tamsulosin has a higher incidence of retrograde ejaculation than placebo, no significant difference was observed in the incidence of other adverse events. CONCLUSION: The results of the current meta-analysis indicated that tamsulosin was superior to placebo in its effi-cacy for distal ureteral stones though retrograde ejaculation was worse with tamsulosin use. It should be a safe and effective medical expulsive therapy choice for distal ureteral stones when stone sizes are less than 10 mm.


Assuntos
Tansulosina/uso terapêutico , Cálculos Ureterais/tratamento farmacológico , Agentes Urológicos/uso terapêutico , Humanos , Placebos , Ensaios Clínicos Controlados Aleatórios como Assunto , Tansulosina/efeitos adversos , Resultado do Tratamento , Cálculos Ureterais/patologia , Agentes Urológicos/efeitos adversos
6.
Cell Death Dis ; 10(4): 307, 2019 04 05.
Artigo em Inglês | MEDLINE | ID: mdl-30952838

RESUMO

Di-n-butyl phthalate (DBP) is a kind of ubiquitous chemical linked to hormonal disruptions that affects male reproductive system. However, the mechanism of DBP-induced germ cells toxicity remains unclear. Here, we demonstrate that DBP induces reduction of proliferation, increase of apoptosis and DNA damage dependent on the PTEN/AKT pathway. Mechanistically, DBP decreases PTEN promoter methylation and increases its transcriptional activity, leading to increased PTEN expression. Notably, DNMT3b is confirmed as a target of miR-29b and miR-29b-mediated status of PTEN methylation is involved in the effects of DBP treatment. Meanwhile, DBP decreases AKT pathway expression via increasing PTEN expression. In addition, the fact that DBP decreases the sperm number and the percentage of motile and progressive sperm is associated with downregulated AKT pathway and sperm flagellum-related genes. Collectively, these findings indicate that DBP induces aberrant PTEN demethylation, leading to inhibition of the AKT pathway, which contributes to the reproductive toxicity.


Assuntos
Desmetilação do DNA , Dibutilftalato/toxicidade , PTEN Fosfo-Hidrolase/metabolismo , Proteínas Proto-Oncogênicas c-akt/metabolismo , Espermatozoides/efeitos dos fármacos , Espermatozoides/metabolismo , Animais , Apoptose/efeitos dos fármacos , Linhagem Celular , Proliferação de Células/efeitos dos fármacos , DNA (Citosina-5-)-Metiltransferases/genética , DNA (Citosina-5-)-Metiltransferases/metabolismo , Dano ao DNA/efeitos dos fármacos , Flagelos/genética , Flagelos/metabolismo , Masculino , Camundongos , Camundongos Endogâmicos C57BL , MicroRNAs/genética , MicroRNAs/metabolismo , PTEN Fosfo-Hidrolase/química , PTEN Fosfo-Hidrolase/genética , Regiões Promotoras Genéticas , Proteínas Proto-Oncogênicas c-akt/antagonistas & inibidores , Proteínas Proto-Oncogênicas c-akt/genética , Transdução de Sinais/efeitos dos fármacos , Motilidade Espermática/efeitos dos fármacos , Motilidade Espermática/genética , Testículo/citologia , Testículo/efeitos dos fármacos , Testículo/metabolismo
7.
Biosci Rep ; 39(6)2019 06 28.
Artigo em Inglês | MEDLINE | ID: mdl-30975733

RESUMO

Autism is known as a severe neurobehavioral syndrome, with males affected more often than females. Previous studies have revealed that microRNAs (miRNAs) play a critical role in the search for novel therapeutic strategies for autism. Therefore, we evaluate the ability of miR-153 to influence brain-derived neurotrophic factor (BDNF) of autism as well as proliferation and apoptosis of hippocampal neuron through the janus kinase-signal transducer and activator of transcription (JAK-STAT) signaling pathway by targeting leptin receptor (LEPR). Firstly, the autistic mice models were established and Morris water maze was employed for the analysis of the learning ability and memory of the mice. Besides, in vitro experiments were conducted with the transfection of different mimic, inhibitor, or siRNA into the hippocampal neuron cells, after which the effect of miR-153 on LEPR and the JAK-STAT signaling pathway-related factors was investigated. Next, 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide assay and flow cytometry assay were conducted to evaluate cell proliferation, cell cycle, and apoptosis respectively following transfection. The results revealed that there was a significant decrease in learning ability and memory in the autistic mice along with a reduction in the positive expression rate of BDNF and serious inflammatory reaction. LEPR was confirmed as a target gene of miR-153 by the dual luciferase reporter gene assay. After transfection of overexpressed miR-153, LEPR and the JAK-STAT signaling pathway were inhibited followed by an increase in BDNF and enhancement of cell proliferation. In conclusion, the high expression of miR-153 can inhibit activation of JAK-STAT signaling pathway by LEPR, thus improving BDNF expression and the proliferative ability of hippocampal neurons.


Assuntos
Transtorno Autístico/genética , Fator Neurotrófico Derivado do Encéfalo/genética , MicroRNAs/genética , Receptores para Leptina/genética , Animais , Apoptose/genética , Transtorno Autístico/patologia , Ciclo Celular/genética , Proliferação de Células/genética , Modelos Animais de Doenças , Feminino , Regulação da Expressão Gênica/genética , Hipocampo/metabolismo , Hipocampo/patologia , Humanos , Janus Quinases/genética , Camundongos , Neurônios/metabolismo , RNA Interferente Pequeno/genética , Fatores de Transcrição STAT
8.
Pol J Microbiol ; 67(4): 417-430, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-30550228

RESUMO

We isolated three laccase-producing fungus strains from Taxus rhizosphere. Myrotheium verrucaria strain DJTU-sh7 had the highest laccase activity of 216.2 U/ml, which was increased to above 300 U/ml after optimization. DJTU-sh7 had the best decolorizing effect for three classes of reactive dyes. The DJTU-sh7-containing fungal consortium displayed the robust decolorizing ability. Both color removal efficiency and chemical oxygen demand were increased in the consortium mediated biotransformation. Transcriptome changes of M. verrucaria elicited by azo dye and phenolic were quantified by the high throughput transcriptome sequencing, and the activities of the selected oxidases and reductases were determined. The possible involvement of oxidases and reductases, especially laccase, aryl alcohol oxidase, and ferric reductase in the biotransformation of dye and phenolic compounds was revealed at both transcriptomic and phenotypic levels. Revealing the transcriptomic mechanisms of fungi in dealing with organic pollutants facilitates the fine-tuned manipulation of strains in developing novel bioremediation and biodegradation strategies.We isolated three laccase-producing fungus strains from Taxus rhizosphere. Myrotheium verrucaria strain DJTU-sh7 had the highest laccase activity of 216.2 U/ml, which was increased to above 300 U/ml after optimization. DJTU-sh7 had the best decolorizing effect for three classes of reactive dyes. The DJTU-sh7-containing fungal consortium displayed the robust decolorizing ability. Both color removal efficiency and chemical oxygen demand were increased in the consortium mediated biotransformation. Transcriptome changes of M. verrucaria elicited by azo dye and phenolic were quantified by the high throughput transcriptome sequencing, and the activities of the selected oxidases and reductases were determined. The possible involvement of oxidases and reductases, especially laccase, aryl alcohol oxidase, and ferric reductase in the biotransformation of dye and phenolic compounds was revealed at both transcriptomic and phenotypic levels. Revealing the transcriptomic mechanisms of fungi in dealing with organic pollutants facilitates the fine-tuned manipulation of strains in developing novel bioremediation and biodegradation strategies.


Assuntos
Ascomicetos/genética , Ascomicetos/metabolismo , Corantes/metabolismo , Rizosfera , Taxus/microbiologia , Transcriptoma , Compostos Azo , Biodegradação Ambiental , Biotransformação , Sequenciamento de Nucleotídeos em Larga Escala , Lacase/biossíntese , Fenóis/metabolismo , Fenótipo , Microbiologia do Solo
9.
Trop Med Infect Dis ; 3(4)2018 Dec 15.
Artigo em Inglês | MEDLINE | ID: mdl-30558259

RESUMO

Schistosoma infection in snails can be monitored by microscopy or indirectly by sentinel mice. As both these approaches can miss infections, more sensitive tests are needed, particularly in low-level transmission settings. In this study, loop-mediated isothermal amplification (LAMP) technique, designed to detect a specific 28S ribosomal Schistosoma japonicum (Sj28S) gene with high sensitivity, was compared to microscopy using snail samples from 51 areas endemic for schistosomiasis in five Chinese provinces. In addition, the results were compared with those from polymerase chain reaction (PCR) by adding DNA sequencing as a reference. The testing of pooled snail samples with the LAMP assay showed that a dilution factor of 1/50, i.e., one infected snail plus 49 non-infected ones, would still result in a positive reaction after the recommended number of amplification cycles. Testing a total of 232 pooled samples, emanating from 4006 snail specimens, showed a rate of infection of 6.5%, while traditional microscopy found only 0.4% positive samples in the same materials. Parallel PCR analysis confirmed the diagnostic accuracy of the LAMP assay, with DNA sequencing even giving LAMP a slight lead. Microscopy and the LAMP test were carried out at local schistosomiasis-control stations, demonstrating that the potential of the latter assay to serve as a point-of-care (POC) test with results available within 60⁻90 min, while the more complicated PCR test had to be carried out at the National Institute of Parasitic Diseases (NIPD) in Shanghai, China. In conclusion, LAMP was found to be clearly superior to microscopy and as good as, or better than, PCR. As it can be used under field conditions and requires less time than other techniques, LAMP testing would improve and accelerate schistosomiasis control.

10.
Infect Dis Poverty ; 7(1): 29, 2018 Mar 26.
Artigo em Inglês | MEDLINE | ID: mdl-29615124

RESUMO

BACKGROUND: Snail intermediate hosts play active roles in the transmission of snail-borne trematode infections in Africa. A good knowledge of snail-borne diseases epidemiology particularly snail intermediate host populations would provide the necessary impetus to complementing existing control strategy. MAIN BODY: This review highlights the importance of molecular approaches in differentiating snail hosts population structure and the need to provide adequate information on snail host populations by updating snail hosts genome database for Africa, in order to equip different stakeholders with adequate information on the ecology of snail intermediate hosts and their roles in the transmission of different diseases. Also, we identify the gaps and areas where there is need for urgent intervention to facilitate effective integrated control of schistosomiasis and other snail-borne trematode infections. CONCLUSIONS: Prioritizing snail studies, especially snail differentiation using molecular tools will boost disease surveillance and also enhance efficient schistosomaisis control programme in Africa.


Assuntos
Vetores de Doenças , Genoma , Caramujos/classificação , Caramujos/genética , Infecções por Trematódeos , África , Animais , Vetores de Doenças/classificação , Humanos , Esquistossomose/epidemiologia , Esquistossomose/prevenção & controle , Esquistossomose/transmissão , Caramujos/parasitologia , Trematódeos/fisiologia , Infecções por Trematódeos/epidemiologia , Infecções por Trematódeos/prevenção & controle , Infecções por Trematódeos/transmissão
12.
Environ Toxicol ; 32(7): 1908-1917, 2017 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-28295950

RESUMO

di-N-butylphthalate (DBP) is a ubiquitous environmental pollutant used for plastic coating and in the cosmetics industry. It has toxic effects on body health, especially the male reproductive system. Here, we investigated the effects of DBP on the male reproductive system of pubertal mice and explored the protective role of sulforaphane (SFN). The results showed that DBP significantly reduced the anogenital distance, testicular weight, sperm count and motility, and plasma and testicular testosterone levels and significantly increased the oxidative stress, sperm abnormalities, and testicular cell apoptosis. SFN supplementation ameliorated these effects. After DBP stimulation, the transcription factor nuclear factor erythroid-related factor 2 (Nrf2) was adaptively increased together with its target genes, such as HO-1 and NQO1. Upregulation of Nrf2 by SFN reduced the DBP-mediated intracellular oxidative toxicity and also increased testosterone secretion and spermatogenesis, which were decreased by DBP. These findings indicate that SFN can attenuate DBP-induced reproductive damage in pubertal mice via Nrf2-associated pathways.


Assuntos
Dibutilftalato/toxicidade , Poluentes Ambientais/toxicidade , Isotiocianatos/farmacologia , Fator 2 Relacionado a NF-E2/metabolismo , Reprodução/efeitos dos fármacos , Animais , Antioxidantes/metabolismo , Apoptose/efeitos dos fármacos , Masculino , Camundongos , Oxirredução , Estresse Oxidativo/efeitos dos fármacos , Contagem de Espermatozoides , Motilidade Espermática/efeitos dos fármacos , Testículo/citologia , Testículo/efeitos dos fármacos , Regulação para Cima
13.
Oncotarget ; 8(30): 50124-50132, 2017 Jul 25.
Artigo em Inglês | MEDLINE | ID: mdl-28223548

RESUMO

Single nucleotide polymorphisms (SNPs) of Excision repair cross-complementing group 2 (ERCC2) gene are suspected to affect the risk of pancreatic cancer. Many studies have reported the association between ERCC2 Lys751Gln polymorphism (rs13181) and the susceptibility to pancreatic cancer, but the outcomes remained controversial. To comprehensively determine this association, we conducted a meta-analysis based on a total of eight studies. Evidence for this association was obtained from the PubMed, EMBASE, Web of Science and Chinese National Knowledge Infrastructure (CNKI) databases. In general, a significant association was found between ERCC2 rs13181 polymorphism and the susceptibility to pancreatic cancer in four genetic models [CC vs. AA: OR = 1.56, (95% CI: 1.28-1.90), P = 0.470; AC/CC vs. AA: OR=1.20, (95% CI: 1.06-1.36), P = 0.396; CC vs. AC/CC: OR = 1.50; (95% CI: 1.24-1.81), P = 0.530; C vs. A: OR=1.22, (95%CI:1.11-1.34), P = 0.159]. Furthermore, stratified analyses by ethnicity indicated a significant association only in the Asian population. Our results indicate that the ERCC2 Lys751Gln polymorphism might be important in stimulating the development of pancreatic cancer, especially for Asians.


Assuntos
Grupo com Ancestrais do Continente Asiático/genética , Neoplasias Pancreáticas/genética , Proteína Grupo D do Xeroderma Pigmentoso/genética , Predisposição Genética para Doença , Humanos , Neoplasias Pancreáticas/patologia , Polimorfismo de Nucleotídeo Único
14.
Fa Yi Xue Za Zhi ; 32(2): 86-9, 93, 2016 Apr.
Artigo em Chinês | MEDLINE | ID: mdl-27501676

RESUMO

OBJECTIVE: To explore the application value of postmortem multi-slice spiral computed tomography (MSCT) by observing and analyzing the injury features in the traffic accident victims. METHODS: Ten traffic accident victims were scanned with whole body MSCT. The systemic autopsy was subsequently performed to compare with the results of MSCT. The advantages and disadvantages of autopsy and MSCT for obtaining the information of traffic accident injuries were then analyzed. RESULTS: MSCT could reveal 3D shape of fractures clearly and detect air accumulation in different positions of the body, which showed the obvious advantages compared with autopsy. However, the resolution of MSCT was limited compared to the detection of organ and soft tissue injuries. CONCLUSION: A combination of MSCT and autopsy is the best way for determining the manner and the cause of death in traffic fatality victims.


Assuntos
Acidentes de Trânsito , Autopsia , Tomografia Computadorizada Espiral , Fraturas Ósseas , Humanos , Lesões dos Tecidos Moles
15.
Fa Yi Xue Za Zhi ; 32(1): 69-73, 2016 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-27295861

RESUMO

A case of a stillbirth with lethal type II osteogenesis imperfecta (OI) was reported. The fetus had skull fractures and craniocerebral injuries during pregnancy. Postmortem multi-sliced computed tomography (MSCT) and 3D-reconstruction were performed, followed by a medico-legal autopsy. The autopsic findings showed the typical features of type II OI, including a soft calvarium, deformed extremities, flexed and abducted hips, and uncommon features, such as white sclera, coxa vara, absence of several bones and organs, a cleft lip, and asymmetric ears. The radiologic images revealed such anomalies and variations as a cleft palate, mandibular dysplasia, spina bifida, costa cervicalis, and fusion of the ribs and vertebrae, which were difficult to detect during conventional autopsy. The paper investigated the classification, causative mutation, cause of death, and the differentiation of OI from child abuse, coming to a conclusion that OI knowledge can be of great importance to forensic pathologists and that the merits of postmortem MSCT should be emphasized in forensic pathologic examinations.


Assuntos
Tomografia Computadorizada Multidetectores/métodos , Osteogênese Imperfeita/diagnóstico , Autopsia , Criança , Morte , Evolução Fatal , Feminino , Feto , Patologia Legal , Humanos , Recém-Nascido , Osteogênese Imperfeita/fisiopatologia , Gravidez , Costelas , Crânio/patologia
16.
Artigo em Chinês | MEDLINE | ID: mdl-30129738

RESUMO

Small non-coding RNA controls the expression of target genes and is related with mRNA degradation, chromatin modification and genome stability. Recent studies showed that small non-coding RNA is not only associated with the incidence of schistosomiasis, but also acts as a potential biomarker. In this article, we will review the applications of small non-coding RNA in schistosomiasis diagnosis and its potential role in schistosome development and pathogenesis, in the aim to provide hints for developing detection methods and vaccines for schistosomiasis and for drug development.


Assuntos
Schistosoma , Esquistossomose , Animais , Humanos , Pequeno RNA não Traduzido , Vacinas
17.
Artigo em Chinês | MEDLINE | ID: mdl-30130012

RESUMO

Objective: To evaluate the transmission risk and features of schistosomiasis by analyzing the data concerning positive rates of anti-schistosome antibody using catalytic models. Methods: Cross-sectional survey was conducted in seven villages with different endemicity of schistosomiasis in Jiangxi (Caohui, Xinhua, Jingtou villages), Anhui (Yuye, Tieguai, Longshang villages) and Hubei (Hebei village) Provinces in 2008. Serum samples were collected and indirect hemagglutination assay was performed to detect anti-schistosome antibodies in serum. Antibody positive rate was calculated and differences among villages and age groups as well as between genders were analyzed. Data of antibody positive rate based on age strata for each village were analyzed by two-stage catalytic model and reversible and two-stage compound catalytic model. Parameters of each model were estimated through the maximum likelihood method. Seroconversion rate and sero-negative conversion rate were estimated in the two-stage catalytic model. Seroconversion rate, seroreversion rate and sero-negative conversion rate were estimated in reversible and two-stage compound catalytic model. The fitting effect was evaluated through correlation analysis and chi-square tests. The best fitted models and parameters were used to analyze the transmission risk and characteristics of schistosomiasis. Results: A total of 6 428 individuals were examined with an average schistosomiasis antibody positive rate of 39.80%(2 485/6 428). In terms of age, the peak of antibody positive rate in Caohui, Xinhua, Jingtou, Yuye, Tieguai, Longshang, and Hebei villages occurred in the age group of 46-50 (82.86%, 58/70), 31-35 (60.78%, 31/51), 31-35 (68.42%, 26/38), 41-45(55.04%, 71/129), 51-55 (62.38%, 63/101), 56-60 (31.43%, 33/105), and 16-20 (21.88%, 7/32) years old, respectively. In general, the antibody positive rate showed a trend of increase followed by a decrease with increasing age in each village. The best model for the data of Caohui, Xinhua and Jingtou and Yuye village was the two-stage catalytic model. The estimated seroconversion rate in these villages was 0.049 5, 0.044 0, 0.055 7, and 0.034 4 respectively, all higher than the corresponding sero-negative conversion rate of 0.005 9, 0.019 6, 0.015 5, and 0.017 8. The best model for the data of Longshang, Tieguai and Hebei villages was the reversible and two-stage compound model. The seroconversion rate in these villages was 0.062 9, 0.168 1, and 0.039 4 respectively, the seroreversion rate was 0.168 8, 0.121 1, and 0.152 2, and the sero-negative conversion rate was 0.001 7, 0.000 2, and 0.090 9. Conclusions: The catalytic model based on antibody positive rate by age strata could reflect the transmission rate and risk quantitatively and may provide guidance for making control strategies.


Assuntos
Schistosoma , Esquistossomose , Animais , Anticorpos Anti-Helmínticos , Biocatálise , China , Estudos Transversais , Testes de Hemaglutinação , Humanos , Medição de Risco
18.
Artigo em Chinês | MEDLINE | ID: mdl-30146861

RESUMO

Objective: To investigate the resistance of E77.43 gene of Microtus fortis(MfE77.43) to Schistosoma japonicum infection. Methods: MfE77.43 was constructed into the recombinant Adeno-associated virus AAV2. The AAV2-MfE77.43 was transfected into HEK293 cells by the calcium phosphate DNA coprecipitation method. The recombinant rAAV2-MfE77.43 was purified and total RNA was extracted from the transfected cells. The expression of E77.43 was examined by RT-PCR and the purity of rAAV2-MfE77.43 was analyzed by SDS-PAGE. Eighteen KM mice were divided into three groups (n=6 in each group). Mice in the experiment group were intramuscularly injected on days 0, 3 and 7 with 400 µl recombinant AAV2-MfE77.43 virus which was 5-fold diluted in normal saline. Mice in negative control and blank control groups received same volume of pAAV or normal saline. Venous blood was collected through the tail before each injection, and E77.43 expression in plasma was detected by dot-ELISA method. After the last injection, each mouse was infected with 40 S. japonicum cercariae and sacrificed on day 41 after infection. Adult worms and liver eggs per gram(LEPG) were counted. Worm and egg reduction rate was calculated respectively. Egg granulomas were observed by HE staining. Results: RT-PCR resulted in a 330 bp specific band. SDS-PAGE of virus shell protein revealed three protein bands with M(r) of 87 000, 72 000 and 62 000, respectively. Dot-ELISA showed that E77.43 protein began to be expressed on day 3 after rAAV2-MfE77.43 injection, remaining stable till day 41. The adult worm number and LEPG were 20.16±3.93 and 19 800±2 715, respectively, with a worm and egg reduction rate of 27.3% and 26.2% in the experiment group. While the worm number and LEPG in the negative control group were 29.16±2.44 and 28 000±2 192(P<0.01), respectively. HE staining and observation revealed fewer eosinophils and inflammatory cells around the liver eggs in the therapy group. Conclusion: The E77.43 gene shows protective effects against S. japonicum infection, indicating that E77.43 may participate in the natural resistance of Microtus fortis to S. japonicum infection.


Assuntos
Schistosoma japonicum , Esquistossomose Japônica , Animais , Arvicolinae , Cercárias , Granuloma , Células HEK293 , Humanos , Fígado , Camundongos , Camundongos Endogâmicos BALB C
19.
Fa Yi Xue Za Zhi ; 31(4): 257-61, 2015 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-26665876

RESUMO

OBJECTIVE: To observe the chemical groups changing in rat kidney with regard to fatal hyperthermia by Fourier transform infrared microspectroscopy (FTIR-MSP) and to provide a new method to diagnose fatal hyperthermia. METHODS: Rats were sacrificed by hyperthermia, brainstem injury, massive hemorrhage and asphyxiation and divided into groups. The renal samples were dissected immediately after death. The data of infrared spectroscopy in glomerulus were measured by FTIR-MSP. RESULTS: The absorbances of 3290, 3070, 2850, 1540 and 1396 cm(-1) significantly increased (P < 0.05), and the ratios of Al650/A3290 and A1650/A1540 significantly decreased (P < 0.05) in group of hyperthermia. CONCLUSION: FTIR-MSP can analyze the changes of chemical groups of kidney as an auxiliary diagnosis for discriminating hyperthermia with other causes of death.


Assuntos
Febre/mortalidade , Rim/metabolismo , Espectroscopia de Infravermelho com Transformada de Fourier/métodos , Animais , Análise de Fourier , Microespectrofotometria , Ratos
20.
Fa Yi Xue Za Zhi ; 31(4): 287-92, 297, 2015 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-26665883

RESUMO

Postmortem chemistry is becoming more and more essential in routine forensic pathology and has made considerable progress over the past years. Biochemical analyses of vitreous humor, blood, urine and cerebrospinal fluid may provide important information in determining the cause of death or in elucidating forensic issues. Postmortem chemistry may be essential for the determination of cause of death when morphological methods (diabetes mellitus, alcoholic ketoacidosis and electrolytic disorders) cannot detect the pathophysiological changes involved in the death process. It can also provide many information in other forensic situations, including myocardial ischemia, sepsis, inflammation, infection, anaphylaxis and hormonal disturbances. The most recent relevant research advances on glucose metabolism, liver function, cardiac function, renal function, sepsis, inflammation, infection, anaphylaxis and hormonal aspect are hereby reviewed.


Assuntos
Autopsia/métodos , Líquidos Corporais/química , Patologia Legal/métodos , Mudanças Depois da Morte , Anafilaxia , Autopsia/tendências , Biomarcadores/análise , Morte , Diabetes Mellitus , Humanos , Sepse , Corpo Vítreo
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