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1.
Comput Intell Neurosci ; 2022: 3428078, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36082353

RESUMO

In the context of global science and technology, all countries pay more and more attention to the text analysis of emotional intonation, and the emotional intonation text analysis and policy orientation of enterprise management in major international and domestic enterprises have also changed from shallow to deep. In the twenty-first century, with the rapid development of human society, people's demand for living standards and material needs increases rapidly, and employees' awareness and needs for work are constantly changing. At present, there is the problem of emotional intonation text analysis error in the management of the enterprise, and the task and emotional transmission command are not clear and thorough. It is necessary to reasonably use deep-learning-related algorithms, especially convolutional neural network and other algorithms, to study the emotional intonation text analysis and policy guidance of the enterprise management. Aiming at the forefront of deep learning development, the latest deep learning technologies are constantly introduced. The research field of emotional intonation text analysis and policy orientation of enterprise management is focused. Through simulation experiment, the characteristics of emotional intonation text analysis and policy orientation research of different enterprise management are compared and analyzed, so as to further improve the emotional intonation text analysis and policy orientation of deep learning for enterprise management.


Assuntos
Aprendizado Profundo , Algoritmos , Emoções , Humanos , Redes Neurais de Computação , Políticas
2.
Front Oncol ; 12: 815998, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36072787

RESUMO

The Disheveled, EGL-10, Pleckstrin domain containing 1 (DEPDC1) is a new oncogene that has recently been described. The mechanisms and functions of its expression are yet to be determined in oral squamous cell carcinoma (OSCC). In the present study, the impact of DEPDC1 on the growth and development of OSCC was investigated using animal models, cell lines and human tissue samples. Elevated DEPDC1 expression within cancer cell lines and human OSCC has been identified. Mechanistic examination showed that restored DEPDC1 expression in vivo and in vitro stimulated OSCC tumour development. In addition, FOXM1 interacts with DEPDC1 as indicated by co-immunoprecipitation and immunofluorescence testing. Functionally, DEPDC1 facilitated Wnt/ß-catenin signal transduction and ß-catenin protein nuclear expression. In summary, the DEPDC1, interacting with FOXM1 via Wnt/ß-catenin signaling, the closely regulated OSCC pathogenesis, suggesting that targeting the novel DEPDC1/FOXM1/ß-catenin complex is an essential OSCC therapeutic approach.

3.
Front Oncol ; 12: 968202, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36059627

RESUMO

Background: Postoperative recurrence impedes the curability of early-stage hepatocellular carcinoma (E-HCC). We aimed to establish a novel recurrence-related pathological prognosticator with artificial intelligence, and investigate the relationship between pathological features and the local immunological microenvironment. Methods: A total of 576 whole-slide images (WSIs) were collected from 547 patients with E-HCC in the Zhongshan cohort, which was randomly divided into a training cohort and a validation cohort. The external validation cohort comprised 147 Tumor Node Metastasis (TNM) stage I patients from The Cancer Genome Atlas (TCGA) database. Six types of HCC tissues were identified by a weakly supervised convolutional neural network. A recurrence-related histological score (HS) was constructed and validated. The correlation between immune microenvironment and HS was evaluated through extensive immunohistochemical data. Results: The overall classification accuracy of HCC tissues was 94.17%. The C-indexes of HS in the training, validation and TCGA cohorts were 0.804, 0.739 and 0.708, respectively. Multivariate analysis showed that the HS (HR= 4.05, 95% CI: 3.40-4.84) was an independent predictor for recurrence-free survival. Patients in HS high-risk group had elevated preoperative alpha-fetoprotein levels, poorer tumor differentiation and a higher proportion of microvascular invasion. The immunohistochemistry data linked the HS to local immune cell infiltration. HS was positively correlated with the expression level of peritumoral CD14+ cells (p= 0.013), and negatively with the intratumoral CD8+ cells (p< 0.001). Conclusions: The study established a novel histological score that predicted short-term and long-term recurrence for E-HCCs using deep learning, which could facilitate clinical decision making in recurrence prediction and management.

4.
Sci Total Environ ; 851(Pt 1): 157974, 2022 Aug 11.
Artigo em Inglês | MEDLINE | ID: mdl-35963407

RESUMO

The unease of consumers with bisphenol A has led to the increased industrial usage of bisphenol F (BPF), which is a new hazard to environmental health. Here, zebrafish were exposed to three BPF concentrations (0.5, 5, and 50 µg/L) from the embryonic stage for 180 days. Results showed that zebrafish body length and weight decreased and hepatosomatic index values increased, even at environmentally relevant concentration. Histological analysis identified the occurrence of hepatic fibrosis and steatosis in 5 and 50 µg/L groups, which indicated the liver injury caused by BPF. Based on the untargeted metabolomics results, a dose-dependent variation in the effects of BPF on liver metabolism was found, and amino acids, purines and one carbon metabolism were the main affected processes in the 0.5, 5, and 50 µg/L treatments, respectively. At the same time, BPF induced a shift in intestinal microbiome composition, including decreased abundance of Erysipelotrichaceae, Rhodobacteraceae and Gemmobacter. In addition, the correlation analysis suggested an association between gut microbiome changes and affected hepatic metabolites after BPF exposure. These findings indicate that a liver-gut alteration is induced by long-term BPF exposure.

5.
Nanomaterials (Basel) ; 12(16)2022 Aug 16.
Artigo em Inglês | MEDLINE | ID: mdl-36014672

RESUMO

Nanomechanical resonators offer brilliant mass and force sensitivity applied in many fields, owing to a low mass m and high-quality factor Q. However, in vibrating process, resonant energy is inevitably dissipated. Typically, quality factor does not surpass the inverse of the material loss angle φ. Recently, some exceptions emerged in the use of highly stressed silicon nitride material. As yet, it is interpreted that the pre-stress seems to "dilute" the intrinsic energy dissipation according to the Zener model. Is there any other material that could further break the 1/φ limit and achieve higher quality factors? In our previous research, through theoretical calculation and finite element simulation, we have proved that graphene's quality factor is two orders of magnitude larger than silicon nitride, on account of the extremely thin thickness of graphene. Based on this, we further optimize the structure of phononic crystals to achieve higher quality factors, in terms of duty cycle and cell size. Through simulation analysis, the quality factor could improve with a larger duty cycle and bigger cell size of triangular lattice phononic crystal. Unexpectedly, the Q amplification coefficient of the 3 × 5-cell structure, which is the least number to compose a phononic crystal with a central defect area, is the highest. In contrast, the minimal cell-number structure in hexagonal lattice could not achieve the brilliant dissipation dilution effect as well as the triangular one. Then we consider how overall size and stress influence quality factor and, furthermore, compare theoretical calculation and finite simulation. Lastly, we start from the primitive 3 × 5 cells, constantly adding cells to the periphery. Through simulation, to our surprise, the largest Q amplification coefficient does not belong to the largest structure, instead originating from the moderate one consisting of 7 × 13 cells.

6.
Ying Yong Sheng Tai Xue Bao ; 33(8): 2027-2034, 2022 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-36043807

RESUMO

In this study, the spatial distribution patterns of individuals with different diameter classes of dominant shrub Lonicera fragrantissima var. lancifolia and the intraspecific and interspecific relationships were analyzed in Qinling Huangguan Plot. The results showed that the diameter class structure of L. fragrantissima var. lancifolia showed a pyramid shape, with a wide bottom and a narrow top. The number of small-diameter class individuals was the largest, showing a good state of renewal and a stable growth, which was conducive to community renewal and succession. Based on Ripley's K function, using univariate and bivariate paired correlation functions, under complete spatial randomness model, heterogeneous Poisson model and antecedent condition model, all individual of the species and their diameter-dividing classes were mainly aggregated, and the aggregation degree decreased with the increases of research scale, and gradually tended to random distribution. Affected by habitat heterogeneity, diffusion restriction and negative density dependence, there was a positive correlation among different intraspecific dia-meter classes, and also a certain degree of no correlation, but without negative correlation. The interspecific relationship was complex. All types of association (no, positive and negative) were observed, but negative association and no association were dominated.


Assuntos
Florestas , Lonicera , China , Ecossistema , Humanos , Árvores
7.
Ying Yong Sheng Tai Xue Bao ; 33(8): 2035-2042, 2022 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-36043808

RESUMO

To explore the spatial distribution and intraspecific correlation of Quercus aliena var. acutiserrata, a domi-nant tree species in a 25 hm2 plot of warm temperate deciduous broadleaved forest in Qinling Mountains, the pair-correlation function g(r) was used to study the spatial pattern and intraspecific association. The results showed that the diameter class structure of Q. aliena var. acutiserrata was bimodal, with a large proportion of young trees (1 cm≤DBH<5 cm), indicating an increase population structure with good capability of regeneration. The abundance of middle trees (15 cm≤DBH<25 cm) was slightly more than that of big trees (25 cm≤DBH<35 cm) and old trees (DBH≥35 cm), but far less than that of young trees and small trees. The spatial distribution of Q. aliena var. acuteserrata was obviously altitude dependent, which mainly distributed in the middle and high altitude areas. Results of complete spatial randomness (CSR) model analysis showed that young trees, small trees, adult trees, big trees, and old trees were aggregated in the large scale (<60 m). Heterogeneous Poisson (HP) model was used to eliminate habitat heterogeneity. The results of HP model showed that the individual aggregation degree of each diameter class decreased, indicating that the distribution was affected by habitat heterogeneity. At the small scale (<40 m), spatial correlation was positively correlated between individuals with small diameter gap, whereas the spatial correlation was negative correlation and no correlation between individuals with large diameter gap. At large scale (>40 m), the spatial correlation was positively correlated between large-diameter individuals, but negatively correlated and unrelated between saplings and other diameter individuals. Our results indicated that biological cha-racteristics of Q. aliena var. acutiserrata and habitat heterogeneity were important drivers for the formation of population spatial pattern.


Assuntos
Quercus , China , Ecossistema , Florestas , Humanos , Árvores
8.
J Ethnopharmacol ; 297: 115550, 2022 Oct 28.
Artigo em Inglês | MEDLINE | ID: mdl-35863612

RESUMO

ETHNOPHARMACOLOGICAL RELEVANCE: Cholestatic liver disease (CLD) is mainly characterized by cholestasis. If not treated, it will deteriorate to cholestatic hepatitis, liver fibrosis, liver cirrhosis, and even liver failure. CLD has a high clinical incidence, and limited treatment with single therapy. In the long-term clinical exploration, traditional Chinese medicine (TCM) has been corroborated with unique therapeutic effects on the CLD process. AIM OF THIS REVIEW: This paper summarizes the effective single and compound TCMs for the treatment of CLD. According to 4 important clinical stages of CLD: cholestasis, hepatitis, liver fibrosis, liver cirrhosis, pharmacological effects and mechanisms of 5 typical TCM examples are reviewed, aims to provide basis for clinical drug selection in different processes of CLD. MATERIALS AND METHODS: Relevant scientific articles regarding therapeutic effects of TCM for the CLD were collected from different databases. We collated three single herbs including Artemisia scoparia Waldst. et Kit. or Artemisia capillaris Thunb. (Artemisiae Scopariae Herba, Yin Chen in Chinese), Paeonia lactiflora Pall. or Paeonia veitchii Lynch. (Paeoniae radix rubra, Chi Shao in Chinese), Poria cocos (Schw.) Wolf (Poria, Fu Ling in Chinese), and two compound herbs of Huang Qi Decoction (HQD) and Yin Chen Hao Decoction (YCHD) to studied and analyzed. RESULTS: We proposed five promising TCMs treatments for the important developmental stages of CLD. Among them, Yin Chen is an essential medicine for protecting liver and gallbladder, and its TCM prescription is also a promising strategy for cholestasis. Based on clinical evidence, high-dose application of Chi Shao is a clinical special treatment of cholestasis hepatitis. Fu Ling can regulate immune cells and increase antibody levels in serum, which is expected to be an emerging therapy to prevent cholestatic liver fibrosis to cirrhosis. HQD can be used as routine clinical medicine for liver fibrosis. In addition, YCHD can exert better comprehensive advantages with multiple components, can treat the whole course of CLD and prevent it from developing to the end-stage. CONCLUSION: Yin Chen, Chi Shao, Fu Ling, HQD and YCHD have shown good clinical efficacy in controlling the development of CLD. Clinically, it is easier to curb the development of CLD by adopting graded diagnosis and treatment measures. We suggest that CLD should be risk stratified in clinical treatment to ensure personalized treatment for patients, so as to slow down the development of the disease.


Assuntos
Artemisia , Colestase , Medicamentos de Ervas Chinesas , Hepatite , Paeonia , Colestase/tratamento farmacológico , Colestase/prevenção & controle , Medicamentos de Ervas Chinesas/farmacologia , Medicamentos de Ervas Chinesas/uso terapêutico , Hepatite/tratamento farmacológico , Cirrose Hepática/tratamento farmacológico , Medicina Tradicional Chinesa
9.
Patterns (N Y) ; 3(7): 100511, 2022 Jul 08.
Artigo em Inglês | MEDLINE | ID: mdl-35845832

RESUMO

Destroying image integrity in scientific papers may result in serious consequences. Inappropriate duplication and fabrication of images are two common misconducts in this aspect. The rapid development of artificial-intelligence technology has brought to us promising image-generation models that can produce realistic fake images. Here, we show that such advanced generative models threaten the publishing system in academia as they may be used to generate fake scientific images that cannot be effectively identified. We demonstrate the disturbing potential of these generative models in synthesizing fake images, plagiarizing existing images, and deliberately modifying images. It is very difficult to identify images generated by these models by visual inspection, image-forensic tools, and detection tools due to the unique paradigm of the generative models for processing images. This perspective reveals vast risks and arouses the vigilance of the scientific community on fake scientific images generated by artificial intelligence (AI) models.

10.
Environ Pollut ; 308: 119716, 2022 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-35809714

RESUMO

Inland freshwater aquaculture ponds (IFAPs) represent the key component of the global lentic freshwater environment and are increasingly important for global aquaculture production, yet the occurrence of organochlorine pesticides (OCPs) in these pond systems remains largely unknown. Here, we characterized the residual concentrations of 19 individual OCPs in sediments and in cultured fish and crustacean species (crabs, shrimp, crayfish and lobster), which were on-spot sampled from the IFAPs at a large region-scale in China. The total OCP levels in sediments varied dramatically between regions. Crabs presented the greatest OCP contamination among the studied species. Dichlorodiphenyltrichloroethanes (DDTs) was the dominating contaminant in sediments and crabs and its stable degradation products 4,4'-DDE and 4,4'-DDD were co-occurrent between these two compartments. The diagnostic ratio analysis indicated fresh inputs of DDTs, lindane and aldrin in multiple regions, which may be resulted from agricultural soil erosion, surface runoff and local anthropogenic activities. Ecological impacts of these pesticides could be expected at some sites due to their levels in sediments above the risk level. Risk assessment based on the OCP levels corrected by the cooking loss revealed that daily consumption of the IFAPs-derived aquatic foods may pose carcinogenic risks in humans.


Assuntos
Hidrocarbonetos Clorados , Praguicidas , Poluentes Químicos da Água , Animais , Aquicultura , China , Monitoramento Ambiental/métodos , Água Doce , Sedimentos Geológicos , Humanos , Hidrocarbonetos Clorados/análise , Praguicidas/análise , Lagoas , Medição de Risco , Poluentes Químicos da Água/análise
11.
Toxicol In Vitro ; 83: 105426, 2022 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-35781001

RESUMO

As commonly used neonicotinoid insecticides for pest control, imidacloprid (IMI) and acetamiprid (ACE) posed neurotoxicity effects on living organisms. However, researches of the differences in toxicity mechanism between these two neonicotinoid insecticides are still limited. In this study, different cellular metabolism perturbations and redox homeostasis damages induced by IMI and ACE exposure in Neuro-2a cells were investigated. Distinct elevation of lactate dehydrogenase (LDH) activity and caspase 7 level demonstrated the influences on necrosis and apoptosis. There were 21 and 12 metabolites screened out as potential biomarkers after IMI and ACE exposure, including lipids and amino acids. Remarkable decrease of lipid hydroperoxides (LOOH) and increase of reactive oxygen species (ROS) generation were found only in the ACE20 group. Interference with glutathione metabolism pathway was further validated by detecting GPx (glutathion peroxidase), GSH (reduced glutathione) and GSSG (oxidized glutathione) levels. Taken together, the metabolic interferences and oxidative damages in ACE20 group were significantly different from the other three exposure groups. These results help to explore the toxicity mechanism of neonicotinoid insecticides from multiple perspectives. This study provides scientific basis for evaluating toxicity of different neonicotinoid insecticides.


Assuntos
Inseticidas , Inseticidas/toxicidade , Lipidômica , Neonicotinoides/toxicidade , Nitrocompostos/toxicidade
12.
Front Psychiatry ; 13: 925007, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35722555

RESUMO

Studies have found that repetitive transcranial magnetic stimulation rTMS can produce antidepressant effects by affecting inflammatory cytokines in patients with depression, which plays a key role in the therapeutic mechanism of antidepressants. This study aimed to explore the changes in inflammatory cytokine levels in patients with depression after 4 weeks of rTMS treatment to determine the possible antidepressant mechanism of rTMS. This prospective, double-blind, pseudo-stimulus-controlled study was conducted, and a total of 57 patients with depression and 30 healthy controls were recruited. Patients were randomly divided into the active rTMS (n = 29) and sham rTMS groups (n = 28). The Hamilton Depression Scale was used to evaluate depressive symptoms and their severity. The serum levels of seven inflammatory cytokines were measured using enzyme-linked immunosorbent assay. Inflammatory cytokines include high-sensitivity C-reactive protein (CRP-hc); tumor necrosis factor (TNF-α); interferon (IFN-γ); interleukin-2 (IL-2); interleukin-4 (IL-4); interleukin-6 (IL-6); and interleukin-8 (IL-8). At baseline, TNF-α (F = 36.699, p < 0.001), IFN-γ (F = 8.907, p < 0.001), IL-4 (F = 66.256, p < 0.001), and IL-2 (F = 9.162, p < 0.001) levels in the depression group were significantly different from those of healthy controls. In the self-control analysis of the active rTMS group, the levels of IL-2 and CRP-hc increased significantly after 2 and 12 weeks of treatment. In the sham-rTMS group, IFN-γ increased after 2 and 12 weeks of treatment. Our results revealed that the changes in inflammatory cytokines after rTMS treatment showed different patterns compared to the sham group, suggesting that the antidepressant effect of rTMS may be related to changes in inflammatory cytokines.

13.
BMC Cardiovasc Disord ; 22(1): 282, 2022 06 22.
Artigo em Inglês | MEDLINE | ID: mdl-35733085

RESUMO

BACKGROUND: The formation of advanced glycation end-products (AGEs) is a crucial risk factor for the pathogenesis of cardiovascular diseases in diabetes. We investigated whether N-epsilon-carboxymethyllysine (CML), a major form of AGEs in vivo, was associated with poor coronary collateral vessel (CCV) formation in patients with type 2 diabetes mellitus (T2DM) and chronic total occlusion (CTO) of coronary artery. METHODS: This study consisted of 242 T2DM patients with coronary angiographically documented CTO. Blood samples were obtained and demographic/clinical characteristics were documented. The coronary collateralization of these patients was defined according to Rentrop or Werner classification. Serum CML levels were evaluated using ELISA assay. Receiver operating characteristic curve and multivariable regression analysis were performed. RESULTS: 242 patients were categorized into poor CCV group or good CCV group (107 vs. 135 by the Rentrop classification or 193 vs. 49 by the Werner classification, respectively). Serum CML levels were significantly higher in poor CCV group than in good CCV group (110.0 ± 83.35 vs. 62.95 ± 58.83 ng/ml by the Rentrop classification and 94.75 ± 78.29 ng/ml vs. 40.37 ± 28.69 ng/ml by Werner classification, both P < 0.001). Moreover, these CML levels were also significantly different across the Rentrop and Werner classification subgroups (P < 0.001). In multivariable logistic regression, CML levels (P < 0.001) remained independent determinants of poor CCV according to the Rentrop or Werner classification after adjustment of traditional risk factors. CONCLUSIONS: This study suggests that higher serum CML level is associated with poor collateralization in T2DM patients with CTO.


Assuntos
Oclusão Coronária , Diabetes Mellitus Tipo 2 , Circulação Colateral , Angiografia Coronária/efeitos adversos , Circulação Coronária , Oclusão Coronária/etiologia , Vasos Coronários/diagnóstico por imagem , Diabetes Mellitus Tipo 2/complicações , Diabetes Mellitus Tipo 2/diagnóstico , Humanos , Lisina/análogos & derivados
14.
J Neural Eng ; 19(3)2022 05 25.
Artigo em Inglês | MEDLINE | ID: mdl-35523144

RESUMO

Objective.A body movement involves the complicated information exchange between the central and peripheral systems, which is characterized by the dynamical coupling patterns between the multiple brain areas and multiple muscle units. How the central and peripheral nerves coordinate multiple internal brain regions and muscle groups is very important when accomplishing the action.Approach.In this study, we extend the adaptive directed transfer function to construct the time-varying networks between multiple corticomuscular regions, and divided the movement duration into different stages by the time-varying corticomuscular network patterns.Main results.The inter dynamical corticomuscular network demonstrated the different interaction patterns between the central and peripheral systems during the different hand movement stages. The muscles transmit bottom-up movement information in the preparation stage, but the brain issues top-down control commands and dominates in the execution stage, and finally the brain's dominant advantage gradually weakens in the relaxation stage. When classifying the different movement stages based on time-varying corticomuscular network indicators, an average accuracy above 74% could be reliably achieved.Significance.The findings of this study help deepen our knowledge of central-peripheral nerve pathways and coordination mechanisms, and also provide opportunities for monitoring and regulating movement disorders.


Assuntos
Eletroencefalografia , Músculo Esquelético , Eletromiografia , Dedos , Movimento/fisiologia , Músculo Esquelético/fisiologia
15.
Front Psychol ; 13: 861823, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35572339

RESUMO

As a worldwide epidemic in the digital age, cyberbullying is a pertinent but understudied concern-especially from the perspective of language. Elucidating the linguistic features of cyberbullying is critical both to preventing it and to cultivating ethical and responsible digital citizens. In this study, a mixed-method approach integrating lexical feature analysis, sentiment polarity analysis, and semantic network analysis was adopted to develop a deeper understanding of cyberbullying language. Five cyberbullying cases on Chinese social media were analyzed to uncover explicit and implicit linguistic features. Results indicated that cyberbullying comments had significantly different linguistic profiles than non-bullying comments and that explicit and implicit bullying were distinct. The content of cases further suggested that cyberbullying language varied in the use of words, types of cyberbullying, and sentiment polarity. These findings offer useful insight for designing automatic cyberbullying detection tools for Chinese social networking platforms. Implications also offer guidance for regulating cyberbullying and fostering ethical and responsible digital citizens.

16.
Environ Sci Technol ; 56(12): 8528-8540, 2022 Jun 21.
Artigo em Inglês | MEDLINE | ID: mdl-35616434

RESUMO

The central nervous system (CNS) is a sensitive target for endocrine-disrupting chemicals, such as bisphenol analogues. Bisphenol A (BPA) usage is associated with the occurrence of many neurological diseases. With the restricted use of BPA, bisphenol F (BPF) has been greatly introduced for industrial manufacture and brings new hazards to public CNS health. To understand how BPF affects the neural system, we performed a cognitive test for zebrafish that are continuously exposed to environmentally relevant concentrations (0.5 and 5.0 µg/L) of BPF since embryonic stage and identified suppressed cognitive ability in adulthood. Single-cell RNA sequencing of neural cells revealed a cell composition shift in zebrafish brain post BPF exposure, including increase in microglia and decrease in neurons; these changes were further validated by immune staining. At the same time, a significant inflammatory response and increased phagocytic activity were detected in zebrafish brain post BPF exposure, which were consistent with the activation of microglia. Cell-specific transcriptomic profiles showed that abnormal phagocytosis, activated brain cell death, and apoptosis occurred in microglia post BPF exposure, which are responsible for the neuron loss. In addition, certain neurological diseases were affected by BPF in both excitatory and inhibitory neurons, such as the movement disorder and neural muscular disease, however, with distinctly involved genes. These findings indicate that BPF exposure could lead to an abnormal cognitive behavior of zebrafish through inducing heterogeneous changes of neural cells in brain and revealed the dominating role of microglia in mediating this effect.


Assuntos
Disruptores Endócrinos , Peixe-Zebra , Animais , Compostos Benzidrílicos/metabolismo , Compostos Benzidrílicos/toxicidade , Cognição , Disruptores Endócrinos/metabolismo , Disruptores Endócrinos/toxicidade , Neurônios , Fenóis , Peixe-Zebra/metabolismo
17.
J Mater Sci Mater Med ; 33(6): 44, 2022 May 16.
Artigo em Inglês | MEDLINE | ID: mdl-35575837

RESUMO

This study investigated the corrosion susceptibility of pure titanium under uric acid exposure for 7 days based on surface analysis. The prepared pure titanium specimens, exposed to different concentrations of uric acid, were examined for surface microstructure, surface element composition and surface wettability using scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS) and static contact angle measurement, respectively. The corrosion behaviors of titanium specimens were measured by open-circuit potential (OCP), electrochemical impedance spectroscopy (EIS) and potentiodynamic polarization. The titanium ion release from the prepared specimens, which were immersed in Hank's balanced salt solution (HBSS) containing different amount of uric acid, was measured by inductively coupled plasma atomic emission spectrometry (ICP-AES). More irregular pitting holes were observed on titanium surfaces exposed to a high concentration of uric acid, and XPS analyses revealed that the amount of titanium dioxide (TiO2) decreased. Titanium surfaces pre-treated with high uric acid became more hydrophobic. Furthermore, the results of OCP and potentiodynamic polarization tests showed increased corrosion susceptibility of titanium samples, while EIS data indicated more active corrosion behavior of titanium materials. The high concentration of uric acid also induced titanium ion release. High concentration of uric acid negatively influenced the surface characteristics and corrosion properties of titanium materials, which destroyed the titanium oxide film barrier. High uric acid exposure increased corrosion susceptibility of pure titanium specimens and accelerated titanium ion release. Graphical abstract.


Assuntos
Titânio , Ácido Úrico , Corrosão , Teste de Materiais , Espectroscopia Fotoeletrônica , Propriedades de Superfície , Titânio/química
18.
Front Bioeng Biotechnol ; 10: 848769, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35528211

RESUMO

Zinc-modified titanium materials have been widely applied in oral implants. Among them, our previous studies have also successfully prepared a novel acid-etched microstructured titanium surface modified with zinc-containing nanowires (Ti-NW-Zn) and proved its excellent biocompatibility. It is well known that the functional regulation between angiogenesis and osteogenesis is of great importance for bone remodeling around implants. However, there are few reports concerning the biological safety of zinc ions released from materials and the appropriate concentration of released zinc ions which was more conducive to angiogenesis and bone regeneration. In this study, we investigated the effects of zinc ions released from Ti-NW-Zn surfaces on angiogenesis and osteogenesis using the zebrafish model and revealed the relationship between angiogenesis and osteogenesis via HUVECs and MC3T3-E1s in vitro. We found that the zinc ions released from Ti-NW-Zn surfaces, with a concentration lower than median lethal concentrations (LCs) of zebrafish, were biologically safe and promote osteogenesis and angiogenesis in vivo. Moreover, the proper concentration of zinc ions could induce the proliferation of HUVECs and osteogenic differentiation. The positive effects of the appropriate concentration of zinc ions on osteoblast behaviors might be regulated by activating the MAPK/ERK signaling pathway. These aspects may provide new sights into the mechanisms underlying zinc-modified titanium surfaces between osteogenesis and angiogenesis, to lay the foundation for further improving the materials, meanwhile, promoting the applications in dentistry.

19.
Environ Int ; 165: 107287, 2022 07.
Artigo em Inglês | MEDLINE | ID: mdl-35598417

RESUMO

The metabolic effects of endocrine-disrupting chemicals, such as bisphenol analogues, have drawn increasing attention. Bisphenol A (BPA) usage is associated with the occurrence of many metabolic diseases. With the restricted use of BPA, alternatives like bisphenol F (BPF) and bisphenol AF (BPAF) have been greatly introduced for industrial manufacture, and brings new hazard to public health. To understand how bisphenol analogues induced metabolic effects, zebrafish are continuous exposed to environmental level (0.5 µg/L) of BPA, BPF and BPAF since embryonic stage, and identified hepatic steatosis and insulin resistance at 60-day post fertilization. Hepatic transcriptional profile indicated that pancreatic disease pathways were activated by BPA, but were inhibited by BPF. At the same time, increased lipid secretion and gluconeogenesis pathways in zebrafish liver was found post BPAF exposure. Significant inflammatory response, histological injury and increased mucus secretion was detected in zebrafish intestine post exposure of three bisphenol analogues. Single-cell RNA sequencing of zebrafish intestinal cells revealed activation of lipid uptake and absorption pathways in enterocyte lineages, which well explained the hepatic steatosis induced by BPA and BPF. Besides, genes related to carbohydrate metabolism, diabetes and insulin resistance were activated in intestinal immune cell types by three bisphenol analogues. These findings indicated that BPA and its alternatives could lead to abnormal lipid and carbohydrate metabolism of zebrafish through inducing cell heterogeneous changes in gut, and revealed both molecular and cellular mechanism in mediating this effect.


Assuntos
Resistência à Insulina , Peixe-Zebra , Animais , Compostos Benzidrílicos/química , Compostos Benzidrílicos/toxicidade , Intestinos , Lipídeos , Fenóis
20.
J Chem Theory Comput ; 18(5): 2803-2815, 2022 May 10.
Artigo em Inglês | MEDLINE | ID: mdl-35380833

RESUMO

We present a multilayer subsystem surface hopping (MSSH) method to deal with nonadiabatic dynamics in large-scale systems. A small subsystem instead of the full system is adopted for surface hopping and is updated on-the-fly to achieve a reliable description of important adiabatic states and the wave function evolution. Additional subsystems for molecular dynamics and statistical description are introduced to further improve the simulation reliability. The global flux hopping probabilities with optimal state assignments are utilized to treat the complex surface crossings. As demonstrated in a series of one- and two-dimensional Holstein models with up to hundreds of thousands of states, MSSH shows weak parameter dependence in all investigated systems. Especially, the computational costs are reduced by 2-6 orders of magnitude compared to traditional surface hopping simulations in full systems, and size-independent results are achieved with a large time-step size of 2-5 fs. The new method is compatible with different decoherence correction strategies and achieves a much better balance between efficiency and reliability, thus promising for applications in general charge and exciton dynamics simulations.

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