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1.
Appl Microbiol Biotechnol ; 105(16-17): 6477-6488, 2021 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-34424384

RESUMO

Co-existence of toxic metals causes complex toxicity to microorganisms during bioremediation in water and soil. This study investigated the immobilization of Pb2+ and Cd2+ by fungus Aspergillus niger, which has been widely applied to environmental remediation. Five treatments were set, i.e., CK (no toxic metals), Pb2+ only, Cd2+ only, Pb2+/Cd2+ = 1:1(molar ratio), and Pb2+/Cd2+ = 2:1. Cadmium induced strong toxicity to the fungus, and maintained the high toxicity during incubation. However, as Pb/Cd ratio increased from 0 to 2, the removal rates of Cd2+ by A. niger were raised from 30 to 50%. The elevated activities of pyruvate dehydrogenase (PDH) and citrate synthetase (CS) enzymes confirmed that Pb addition could stimulate the growth of A. niger. For instance, citric acid concentrations and CS activities were 463.22 mg/L and 78.37 nmol/min/g, respectively, during 3-day incubation as Pb/Cd = 1. However, these two values were as low as ~ 50 with addition of only Cd. It was hence assumed that appropriate co-existence of Pb2+ enhanced microbial activity by promoting TCA cycle of the fungus. Moreover, the SEM analysis and geochemical modeling demonstrated that Pb2+ cations were more easily adsorbed and mineralized on A. niger with respect to Cd2+. Therefore, instead of intensifying metal toxicity, the addition of appropriate Pb actually weakened Cd toxicity to the fungus. This study sheds a bright future on application of A. niger to the remediation of polluted water with co-existence of Pb and Cd. KEY POINTS: • Cd2+ significantly inhibited P consumption, suggesting its high toxicity to A. niger. • Pb2+ stimulated the growth of A. niger by promoting TCA cycle in the cells. • Cd2+ removal by A. niger were improved with co-existence of Pb2+.


Assuntos
Aspergillus niger , Cádmio , Bioacumulação , Biodegradação Ambiental , Cádmio/análise , Cádmio/toxicidade , Chumbo/toxicidade
2.
Cell Death Dis ; 12(7): 708, 2021 07 15.
Artigo em Inglês | MEDLINE | ID: mdl-34267179

RESUMO

The dysregulated microRNAs (miRNAs) are involved in diabetic retinopathy progression. Epithelial mesenchymal transition (EMT) and cell permeability are important events in diabetic retinopathy. However, the function and mechanism of miR-195 in EMT and cell permeability in diabetic retinopathy remain largely unclear. Diabetic retinopathy models were established using streptozotocin (STZ)-induced diabetic mice and high glucose (HG)-stimulated ARPE-19 cells. Retina injury was investigated by hematoxylin-eosin (HE) staining. EMT and cell permeability were analyzed by western blotting, immunofluorescence, wound healing, and FITC-dextran assays. MiR-195 expression was detected via qRT-PCR. YY1, VEGFA, Snail1, and Smurf2 levels were detected via western blotting. The interaction relationship was analyzed via ChIP, Co-IP, or dual-luciferase reporter assay. The retina injury, EMT, and cell permeability were induced in STZ-induced diabetic mice. HG induced EMT and cell permeability in ARPE-19 cells. MiR-195, YY1, VEGFA, and Snail1 levels were enhanced, but Smurf2 abundance was reduced in STZ-induced diabetic mice and HG-stimulated ARPE-19 cells. VEGFA knockdown decreased Snail1 expression and attenuated HG-induced EMT and cell permeability. YY1 silence reduced VEGFA and Snail1 expression, and mitigated HG-induced EMT and cell permeability. YY1 could bind with VEGFA and Snail1, and it was degraded via Smurf2-mediated ubiquitination. MiR-195 knockdown upregulated Smurf2 to decrease YY1 expression and inhibited HG-induced EMT and cell permeability. MiR-195 targeted Smurf2, increased expression of YY1, VEGFA, and Snail1, and promoted HG-induced EMT and cell permeability. MiR-195 promotes EMT and cell permeability of HG-stimulated ARPE-19 cells by increasing VEGFA/Snail1 via inhibiting the Smurf2-mediated ubiquitination of YY1.


Assuntos
Retinopatia Diabética/metabolismo , Células Epiteliais/metabolismo , Transição Epitelial-Mesenquimal , MicroRNAs/metabolismo , Epitélio Pigmentado da Retina/metabolismo , Ubiquitina-Proteína Ligases/metabolismo , Fator de Transcrição YY1/metabolismo , Animais , Linhagem Celular , Retinopatia Diabética/genética , Retinopatia Diabética/patologia , Modelos Animais de Doenças , Células Epiteliais/patologia , Glucose/metabolismo , Humanos , Masculino , Camundongos Endogâmicos C57BL , MicroRNAs/genética , Permeabilidade , Complexo de Endopeptidases do Proteassoma/metabolismo , Proteólise , Epitélio Pigmentado da Retina/patologia , Transdução de Sinais , Fatores de Transcrição da Família Snail/metabolismo , Ubiquitina-Proteína Ligases/genética , Ubiquitinação , Fator A de Crescimento do Endotélio Vascular/genética , Fator A de Crescimento do Endotélio Vascular/metabolismo , Fator de Transcrição YY1/genética
3.
J Physiol Anthropol ; 40(1): 7, 2021 Jun 08.
Artigo em Inglês | MEDLINE | ID: mdl-34103077

RESUMO

BACKGROUND: Blue-enriched white light at night has the potential to delay the circadian rhythm in daily life. This study was conducted to determine whether the use of high correlated color temperature (CCT) light at home at night is associated with delay of sleep timing in university students. METHODS: The survey was conducted in 2014-2015 in 447 university students in Japan and 327 students in China. Habitual sleep timing and type of CCT light at home were investigated by using a self-administered questionnaire. The Japanese students were significantly later than the Chinese students in bedtime, wake time, and midpoint of sleep. They were asked whether the lighting in the room where they spend most of their time at night was closer to warm color (low CCT) or daylight color (high CCT). The amount of light exposure level during daily life was measured for at least 1 week by the use of a light sensor in 60 students in each country. RESULTS: The percentages of participants who used high CCT lighting at night were 61.6% for Japanese students and 80.8% for Chinese students. Bedtime and sleep onset time on school days and free days were significantly later in the high CCT group than in the low CCT group in Japan. The midpoint of sleep in the high CCT group was significantly later than that in the low CCT group on free days but not on school days. On the other hand, none of the sleep measurements on school days and free days were significantly different between the high CCT and low CCT groups in China. Illuminance level of light exposure during the night was significantly higher in Japanese than in Chinese, but that in the morning was significantly higher in China than in Japan. CONCLUSIONS: The use of high CCT light at night is associated with delay of sleep timing in Japanese university students but not in Chinese university students. The effects of light at night on sleep timing and circadian rhythm may be complicated by other lifestyle factors depending on the country.


Assuntos
Ritmo Circadiano/efeitos da radiação , Iluminação/métodos , Sono/efeitos da radiação , Adulto , China , Feminino , Humanos , Japão , Masculino , Estudantes , Inquéritos e Questionários , Fatores de Tempo , Universidades , Adulto Jovem
4.
Environ Microbiol ; 2021 May 18.
Artigo em Inglês | MEDLINE | ID: mdl-34002461

RESUMO

Rhodotorula mucilaginosa resists heavy metal (HM) stress because of its abundant extracellular polymeric substances and functional vesicles. In this study, we provided new insights into its survival strategies at both biochemical and genetic levels. After lead exposure, carotenoid biosynthesis was initiated within 24 h incubation and then increased to the maximum after 96 h of incubation. Raman analysis confirmed that carotenoids (primarily ß-carotene) were the major identifiable chemical substances on the cell surface. Moreover, the increased carotenoid production was accompanied by a rising budding rate, ~40% higher than that in the cultures without Pb. During the 96 h of incubation, the driving force for Pb accumulation was assigned to this elevated budding rate. After 96 h, biosorption was primarily attributed to the enhanced antioxidant ability of the single cells during carotenoid production. Furthermore, the yeast budding cells demonstrated an evidently heterogeneous biosorption of Pb, i.e., the rejuvenated daughters had a relatively lower Pb level than the mother cells. This resulted in the protection of the buds from Pb stress. After investigating phosphorus uptake and the RNA sequencing data, we finally confirmed two tightly correlated pathways that resist HM stress, i.e., biochemical (carotenoid production) and reproductive (healthy buds) pathways.

5.
Artigo em Inglês | MEDLINE | ID: mdl-34032909

RESUMO

OBJECTIVE: A systematic review and meta-analysis were performed to evaluate the prevalence and prognosis of otorhinolaryngological symptoms in patients with the diagnosed coronavirus disease 2019 (COVID-19). METHODS: A systematic search of PubMed, Embase, Web of Science, and Google Scholar databases was performed up to August 19, 2020.We included studies that reported infections with COVID-19 and symptoms of otolaryngology. The retrieved data from the respective studies were evaluated and summarized. The study's immediate result was to assess the combined prevalence of otorhinolaryngological symptoms in patients with COVID-19. However, the secondary result was to determine the exacerbation of COVID-19 infection in patients with otorhinolaryngological symptoms. RESULTS: Fifty-four studies with 16,478 patients were included. Olfactory dysfunction, sneezing and sputum production were the 3 most prevalent otorhinolaryngological symptoms in patients with COVID-19. The pooled prevalence amongst the prevalent symptoms was 47% (95% CI 29-65; range 0-98; I2 = 99.58%), 27% (95% CI 11-48; range 12-40; I2 = 93.34%), and 22% (95% CI 16-30; range 2-56; I2 = 97.60%), respectively. The proportion of severely ill patients with sputum production and shortness of breath was significantly higher among patients with COVID-19 infections (OR 1.66 [95% CI 1.08-2.54]; P = 0.02, I2 = 51% and 3.29 [95% CI 1.57-6.90]; P = 0.002, I2 = 49%, respectively). Subgroup analysis showed no statistically significant differences between the incidence of otolaryngology symptoms in severely ill patients and non-severely ill patients (OR 1.43 [95% CI 1.12-1.82]; P = 0.07 I2 = 53.1%). In contrast, the incidence of shortness of breath in severely ill patients was significantly increased (3.29 [1.57-6.90]; P = 0.002, I2 = 49%). CONCLUSION: Our research shows that otorhinolaryngology symptoms in patients with COVID-19 are not uncommon, which should attract otorhinolaryngologists' attention.

6.
J Asthma ; : 1-7, 2021 Apr 23.
Artigo em Inglês | MEDLINE | ID: mdl-33863266

RESUMO

OBJECTIVE: Previous studies have reported a correlation between coronavirus disease-2019 (COVID-19) and asthma. However, data on whether asthma constitutes a risk factor for COVID-19 and the prevalence of asthma in COVID-19 cases still remain scant. Here, we interrogated and analyzed the association between COVID-19 and asthma. METHODS: In this study, we systematically searched PubMed, Embase, and Web of Science databases for studies published between January 1 and August 28, 2020. We included studies that reported the epidemiological and clinical features of COVID-19 and its prevalence in asthma patients. We excluded reviews, animal trials, single case reports, small case series and studies evaluating other coronavirus-related illnesses. Raw data from the studies were pooled into a meta-analysis. RESULTS: We analyzed findings from 18 studies, including asthma patients with COVID-19. The pooled prevalence of asthma in COVID-19 cases was 0.08 (95% CI, 0.06-0.11), with an overall I2 of 99.07%, p < 0.005. The data indicated that asthma did not increase the risk of developing severe COVID-19 (odds ratio [OR] 1.04 (95% CI, 0.75-1.46) p = 0.28; I2=20%). In addition, there was no significant difference in the incidence of asthma with age in COVID-19 infections [OR] 0.77(95% CI, 0.59-1.00) p = 0.24; I2=29%). CONCLUSION: Taken together, our data suggested that asthma is not a significant risk factor for the development of severe COVID-19.

7.
Medicine (Baltimore) ; 100(12): e25197, 2021 Mar 26.
Artigo em Inglês | MEDLINE | ID: mdl-33761702

RESUMO

BACKGROUND: Type 2 diabetes mellitus (T2D) and osteoporosis (OP) often coexist, and both are systemic metabolic diseases and seriously increase the risk of fragility fracture. However, there is no specific Western medicine for the treatment of T2D with OP (T2DOP). As reported in clinical and experimental studies, traditional Chinese medicine (TCM) based on principle of tonifying-kidney, strengthening-spleen, and invigorating blood circulation (Bushen-Jianpi-Huoxue) (BSJPHX) has significant efficacy against T2DOP. This protocol will be designed for a systematic review and meta-analysis to assess the efficacy and safety of TCM BSJPHX principle in the treatment of T2DOP. METHODS: All relevant randomized controlled trials (RCTs) related to TCM therapies conducted in BSJPHX principle for T2DOP will be searched in the 8 electronic databases: PubMed, Cochrane Library, Wed of Science, EMBASE, Chinese National Knowledge Infrastructure Database (CNKI), Chinese Biomedical Literatures Database (CBM), Chinese Scientific Journal Database (VIP), Wanfang Database, from inception to October 2020. The main outcomes will contain: fasting blood glucose (FPG), 2 hours postprandial blood glucose (2hPG), glycosylated hemoglobin (HbA1c), serum calcium, bone mineral density (BMD), TCM syndrome integral, and the additional outcomes will consist of visual analog scale (VAS), and adverse events. Two reviewers will independently carry out literature search, data selection and synthesis, and literature quality assessment. In case of any dispute, it will be settled by group discussion. Assessment of risk of bias, reporting bias, and data synthesis would be performed with Review Manager software (Rev-Man 5.3). RESULT: This study will collate and summarize the various current evidences of TCM BSJPHX principle for T2DOP. CONCLUSION: This study will offer convincing evidence for judging the efficacy and safety of TCM BSJPHX principle for T2DOP. PROSPERO REGISTRATION NUMBER: CRD42020218877.


Assuntos
Diabetes Mellitus Tipo 2/tratamento farmacológico , Medicamentos de Ervas Chinesas/uso terapêutico , Metanálise como Assunto , Osteoporose/tratamento farmacológico , Revisões Sistemáticas como Assunto , Glicemia/metabolismo , Densidade Óssea , Cálcio/sangue , Diabetes Mellitus Tipo 2/sangue , Diabetes Mellitus Tipo 2/complicações , Medicamentos de Ervas Chinesas/efeitos adversos , Hemoglobina A Glicada/metabolismo , Humanos , Insulina/sangue , Resistência à Insulina , Osteoporose/sangue , Osteoporose/complicações , Projetos de Pesquisa
8.
Free Radic Biol Med ; 169: 361-381, 2021 06.
Artigo em Inglês | MEDLINE | ID: mdl-33762162

RESUMO

Long noncoding RNAs (lncRNAs) are important regulators in various diseases including diabetic retinopathy (DR). In this study, DR patients exhibited significantly increased expression of serum LncRNA-OGRU compared with normal individuals. Streptozotocin (STZ)-challenged rats with DR also had higher OGRU expression in retinas than that of the control group, which was confirmed in Müller cells upon high glucose (HG) stimulation. OGRU knockdown remarkably decreased vascular endothelial growth factor (VEGF) and transforming growth factor-ß1 (TGF-ß1) expression in HG-incubated Müller cells. HG-induced inflammatory response and oxidative stress in vitro were markedly mitigated by OGRU knockdown through restraining IκBɑ/nuclear factor kappa beta (NF-κB) and improving nuclear factor erythroid 2-related factor 2 (Nrf2) signaling pathways, respectively. Further studies indicated that OGRU suppression greatly restored miR-320 expression, and a negative correlation between them was detected in DR patients. We also found that miR-320 over-expression considerably restrained TGF-ß1 signaling, and hindered inflammation and reactive oxygen species (ROS) production in HG-stimulated Müller cells. Additionally, OGRU knockdown or miR-320 over-expression could dramatically down-regulate ubiquitin-specific peptidase 14 (USP14) expression levels in HG-incubated Müller cells, and miR-320 could directly target USP14. Notably, OGRU/miR-320 axis-mediated TGF-ß1 signaling, inflammation and ROS were largely dependent on USP14. Intriguingly, our results showed that USP14 directly interacted with transforming growth factor-beta type 1 receptor (TßR1), and impeded TßR1 ubiquitination and degradation. Furthermore, USP14 could also facilitate IκBɑ deubiquitination and degradation, exacerbating IκBɑ phosphorylation and NF-κB activation. Finally, our in vivo studies confirmed that OGRU knockdown considerably ameliorated DR progression in STZ-challenged rats through mediating the mechanisms observed in vitro. Collectively, these findings implicated that LncRNA-OGRU mediated DR progression through competing for miR-320 to regulate USP14 expression, and thus LncRNA-OGRU/miR-320/USP14 axis may be considered as a therapeutic target for DR treatment.


Assuntos
Diabetes Mellitus , Retinopatia Diabética , MicroRNAs , RNA Longo não Codificante , Animais , Retinopatia Diabética/genética , Humanos , Inflamação/genética , MicroRNAs/genética , MicroRNAs/metabolismo , Estresse Oxidativo/genética , RNA Longo não Codificante/genética , RNA Longo não Codificante/metabolismo , Ratos , Ubiquitina Tiolesterase/genética , Fator A de Crescimento do Endotélio Vascular/metabolismo
9.
Endocr J ; 68(5): 531-541, 2021 May 28.
Artigo em Inglês | MEDLINE | ID: mdl-33563844

RESUMO

This study investigated the effects and mechanisms of miR-132 related to the permeability and mobility of human retinal pigment epithelium ARPE-19 cells in high-glucose (HG) condition. ARPE-19 cells were cultured in normal and HG condition and identified by immunofluorescence staining. Cell viability was assessed by the MTT assay, cell permeability was assessed by the FITC-dextran assay and cell mobility was assessed by the wound healing assay. Different miRNA and mRNA expression levels were determined by quantitative real-time polymerase chain reaction (RT-qPCR). The expression of tight junction-related proteins was determined by Western blot assay and immunofluorescence. The interaction between occludin and miR-132 was confirmed by a dual-luciferase reporter assay. We revealed that HG-treated ARPE-19 cells exhibited significantly increased miR-132 expression, decreased expression of the tight-junction markers including occludin and E-cadherin, and increased cell mobility and permeability. Occludin is a direct target of miR-132, which could regulate cell viability, mobility and permeability under HG condition through the JAK/STAT3 signaling pathway. These are the first data to suggest that miR-132 may contribute to the progression of diabetic retinopathy (DR) and that targeting the effect of miR-132 on occudin and the JAK/STAT3 pathway could represent a novel effective DR-treatment strategy.

10.
Inorg Chem ; 2021 Jan 20.
Artigo em Inglês | MEDLINE | ID: mdl-33471502

RESUMO

Metal-organic frameworks (MOFs) and MOF-based composites as luminescent sensors with excellent economic practicability and handy operability have attracted much attention. Herein, we designed and fabricated a porous Zn-based MOF, [Zn(OBA)2(L1)·2DMA]n [1; H2OBA = 4,4'-oxybis(benzoic acid), L1 = 2,4,6-tris(4-pyridyl)pyridine, and DMA = N,N-dimethylacetamide], with mixed nodes under solvothermal conditions, and the pore size of 5.9 Å was calculated from N2 adsorption isotherms by using a density functional theory model. The as-synthesized compound 1 is stable in different boiling organic solvents and water solutions with a wide pH range of 2-12 and exhibits intense luminescence emission at 360 nm under excitation of 290 nm. Significantly, compound 1 shows high selective detection of Fe3+, CrO42-, and Cr2O72- in aqueous solution even under the interference of other ions. Compound 1 can quickly sense these ions in a short time and has a striking sensitivity toward Fe3+ with an ultralow limit of detection (LOD) of 1.06 µM. The relatively low LODs for CrO42- and Cr2O72- are 3.87 and 2.37 µM, respectively, compared to the reported works. Meanwhile, compound 1 can be reused to detect Fe3+, CrO42-, and Cr2O72- six times by simple regeneration. Considering the practicability, a mixed-matrix membrane (MMM) incorporated compound 1 and poly(methyl methacrylate) has been constructed. This MMM displays quick detection of Fe3+, CrO42-, and Cr2O72- and prompt regeneration by lifting from the analyte. This useful MMM shows a comparable LOD below 4.35 µM for these ions. This work presents a cost-effective Zn-based MOF as a functional platform for simple but useful sensing of Fe3+, CrO42-, and Cr2O72- in aqueous solution.

11.
Sci Total Environ ; 755(Pt 2): 142570, 2021 Feb 10.
Artigo em Inglês | MEDLINE | ID: mdl-33035850

RESUMO

Carbonate in soil from karst region is a substantial carbon sink on Earth. Many karst regions are covered by P-deficient soil. This study evaluated the influences of phosphate addition on fungal weathering (by typical phosphate-solubilizing fungus Aspergillus niger) of carbonate in the soil with red color from karst region. Two weathering pathways were recognized, i.e., biochemical and biomechanical deterioration. The biochemical pathway was performed by dissolving carbonate via secreting organic acids. Meanwhile, the dominant organic acid, i.e., oxalic acid, induced the formation of calcium oxalate, which prevented the loss of Ca2+ cations. It was estimated that the ideal carbonate solubilization driven by geological fluorapatite and fungal weathering is up to 3.3% per year, based on the equation of 12 × (RBase + RPSF) × m × (Areal/APSF). Moreover, fungal weathering of carbonate is very sensitive to the solubility of phosphates. Phosphates supply essential P source for the fungal growth and subsequently raise water-soluble P content in the soil. The addition of bioapatite (a variety of natural apatite with relatively high solubility) elevated the value to 4.6% (a ~ 40% enhancement compared with FAp). This research hence elucidated the tight correlation between carbonate weathering and P supply. Inorganic C release driven by P availability and microbial weathering should be addressed in karst region.


Assuntos
Fosfatos , Solo , Sequestro de Carbono , Carbonatos/análise , Fungos
12.
Water Res ; 182: 115927, 2020 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-32622123

RESUMO

Reuse of sludge-derived Fe-rich biochar as sludge conditioner is an attractive route for management of waste activated sludge at source. Homogeneous and heterogeneous Fenton reactions have been proved in sludge conditioning with Fe-rich biochar activating H2O2 to enhance sludge dewaterability. The FeAl2O4 phase in Fe-rich biochar was first identified during pyrolysis of sewage sludge after adding both Fe2O3 and Al2O3, since Fe and Al elements are two of major metal elements in Fe-rich sludge. Compared with the Fe-rich biochar that did not comprise FeAl2O4 phase, the capillary suction time (CST) and specific resistance to filterability (SRF) of the sludge conditioned with the Fe-rich biochar comprising FeAl2O4 phase could be efficiently decreased by 23% and 44%, respectively. The results indicated that FeAl2O4 phase in Fe-rich biochar could improve sludge dewaterability by enhancing heterogeneous Fenton reaction. Synergistic effect between Fe and Al in FeAl2O4 contributed to weak the O-O bond in H2O2 and reduce the activation energy of H2O2 decomposition for enhancing ·OH generation, which could be explained by density functional theory (DFT) calculations for the first time. Thus, the decomposition rate of H2O2 and the amount of ·OH generation were obviously promoted by FeAl2O4 phase in sludge-derived biochar during sludge conditioning, attributing to the destruction of sludge flocs, the release of bound water, and the improvement of sludge dewaterability.


Assuntos
Peróxido de Hidrogênio , Esgotos , Carvão Vegetal , Ferro , Oxirredução , Eliminação de Resíduos Líquidos , Água
13.
BMC Plant Biol ; 20(1): 215, 2020 May 13.
Artigo em Inglês | MEDLINE | ID: mdl-32404101

RESUMO

BACKGROUND: MicroRNAs are important gene expression regulators in plants immune system. Aspergillus flavus is the most common causal agents of aflatoxin contamination in peanuts, but information on the function of miRNA in peanut-A. flavus interaction is lacking. In this study, the resistant cultivar (GT-C20) and susceptible cultivar (Tifrunner) were used to investigate regulatory roles of miRNAs in response to A. flavus growth. RESULTS: A total of 30 miRNAs, 447 genes and 21 potential miRNA/mRNA pairs were differentially expressed significantly when treated with A. flavus. A total of 62 miRNAs, 451 genes and 44 potential miRNA/mRNA pairs exhibited differential expression profiles between two peanut varieties. Gene Ontology (GO) analysis showed that metabolic-process related GO terms were enriched. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses further supported the GO results, in which many enriched pathways were related with biosynthesis and metabolism, such as biosynthesis of secondary metabolites and metabolic pathways. Correlation analysis of small RNA, transcriptome and degradome indicated that miR156/SPL pairs might regulate the accumulation of flavonoids in resistant and susceptible genotypes. The miR482/2118 family might regulate NBS-LRR gene which had the higher expression level in resistant genotype. These results provided useful information for further understanding the roles of miR156/157/SPL and miR482/2118/NBS-LRR pairs. CONCLUSIONS: Integration analysis of the transcriptome, miRNAome and degradome of resistant and susceptible peanut varieties were performed in this study. The knowledge gained will help to understand the roles of miRNAs of peanut in response to A. flavus.


Assuntos
Arachis/genética , Aspergillus flavus/crescimento & desenvolvimento , Interações Hospedeiro-Patógeno , MicroRNAs/genética , RNA Mensageiro/genética , Transcriptoma , Arachis/metabolismo , Arachis/microbiologia , Genes de Plantas , MicroRNAs/metabolismo , RNA Mensageiro/metabolismo , RNA de Plantas/genética , RNA de Plantas/metabolismo , Sementes/metabolismo , Sementes/microbiologia
14.
Int J Antimicrob Agents ; 56(1): 106018, 2020 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-32417205

RESUMO

INTRODUCTION: This systematic review and meta-analysis investigated the efficacy and safety of single-dose fosfomycin tromethamine (FT) versus other antibiotic agents in women suffering from lower uncomplicated urinary tract infection (uUTI) and pregnant women with uUTI or asymptomatic bacteriuria (ASB). METHODS: MEDLINE, EMBASE and the Cochrane library were searched to identify relevant literature. Twenty-one studies were identified. Nine of the 21 studies enrolled 21 22 patients and were used to compare the clinical resolution of uUTI between non-pregnant and pregnant women. Given that uUTI and ASB are assessed using similar microbiological evaluation methods, all 3103 patients in the identified 21 studies were pooled to determine microbiological resolution between uUTI or ASB patients. Safety outcomes of the treatments were analysed in 15 studies. RESULTS: The results showed that single-dose FT was comparable with other antibiotic agents in clinical resolution of uUTI (OR 0.89; 95% CI 0.71-1.10; P = 0.41) in non-pregnant (P = 0.32) and pregnant women (P = 0.64). Moreover, single-dose FT was equal to other antibiotics in microbiological resolution, and there was no difference in overall microbiological resolution (OR 1.11; 95% CI 0.92-1.34; P = 0.29) among non-pregnant women with uUTI (P = 0.48), pregnant women with uUTI (P = 0.81) and pregnant women with ASB (P = 0.30). There were no serious fosfomycin-related adverse events and most frequent adverse events were mainly gastrointestinal. CONCLUSION: This meta-analysis suggests that single-dose fosfomycin tromethamine produces equivalent clinical outcomes to comparator antibiotics in terms of clinical efficacy and microbiological efficacy. It is therefore clinically effective and safe for women with uUTI and pregnant women with uUTI or ASB, and has higher patient compliance.


Assuntos
Antibacterianos/uso terapêutico , Doenças Assintomáticas/terapia , Bacteriúria/tratamento farmacológico , Fosfomicina/uso terapêutico , Infecções Urinárias/tratamento farmacológico , Antibacterianos/efeitos adversos , Bacteriúria/microbiologia , Feminino , Fosfomicina/efeitos adversos , Humanos , Gravidez , Resultado do Tratamento , Infecções Urinárias/microbiologia
15.
Nan Fang Yi Ke Da Xue Xue Bao ; 40(2): 203-210, 2020 Feb 29.
Artigo em Chinês | MEDLINE | ID: mdl-32376529

RESUMO

OBJECTIVE: To assess the performance of high-performance liquid chromatography (HPLC) combined with capillary monolithic column molecularly imprinted with metal organic frame (UiO-66-NH2@MIPs capillary monolithic column) for enrichment, purification and detection of Ponceau 4R in Carthami flos. METHODS: UiO-66-NH2@MIPs monolithic columns were prepared via in situ polymerization, and the adsorption properties and morphology of the columns were characterized by HPLC, scanning electron microscopy (SEM) and infrared (IR) spectral analysis. HPLC with the prepared columns was performed for detecting the content of Ponceau 4R in Carthami flos samples. RESULTS: The UiO-66-NH2@MIPs system showed a good linearity for detecting Ponceau 4R over the concentration range of 0.1-10.0 µg/mL with a correlation coefficient > 0.9999 and a detection limit (S/N=3) of 2.7×10-4 µg/mL. The mean recovery of Ponceau 4R in Carthami flos samples ranged from 82.60% to 105.56%, and the intra-day and inter-day relative standard deviation (RSD) values ranged from 2.4% to 3.4%. The recycling experiment showed that the system could be reused for sensitive detection of Ponceau 4R in Carthami flos. The capacity of UiO-66-NH2@MIPs column was 0.178 µg/mg, which was superior to that of other monolithic columns (0.089, 0.080, and 0.111 µg/ mg), demonstrating that the addition of UiO-66-NH2 increased the adsorption capacity of the system. Under the optimized conditions, the UiO-66-NH2@MIPs-HPLC system had an enrichment factor of over 73 folds with obviously reduced interference by the impurity peaks. CONCLUSIONS: The UiO-66-NH2@MIPs column-HPLC system has much better performance for enrichment, purification and detection of Ponceau 4R in Carthami flos than direct HPLC.


Assuntos
Cromatografia Líquida de Alta Pressão , Compostos Azo , Metais , Impressão Molecular , Naftalenossulfonatos , Extração em Fase Sólida
16.
Sci Total Environ ; 725: 138299, 2020 Jul 10.
Artigo em Inglês | MEDLINE | ID: mdl-32278183

RESUMO

Fe-rich biochar with multivalent iron compounds (Fe0, Fe0.95C0.05, Fe3O4, and FeAl2O4) pyrolyzed from sludge cake conditioned with Fenton's reagent and red mud was utilized as an efficient Fenton catalyst for the degradation of 4-chlorophenol (4-CP). Effects of pyrolysis temperature and sludge conditioner composition on the transformation of iron compounds were studied. Both homogeneous Fenton reaction initiated by Fe2+ leached from both low-valent Fe0 and Fe0.95C0.05, and heterogeneous Fenton reaction initiated by solid iron phases of Fe3O4 and FeAl2O4 were revealed to contribute to the degradation of 4-CP. The removal efficiency of 4-CP remained 100% after five successive degradation rounds. The homogeneous Fenton reaction mainly works in the first degradation round, and the heterogeneous Fenton reaction dominates in subsequent degradation rounds. The findings of this study suggest that sewage sludge derived Fe-rich biochar could be utilized as an efficient Fenton catalyst for recalcitrant organics degradation.


Assuntos
Carvão Vegetal , Esgotos , Catálise , Clorofenóis , Peróxido de Hidrogênio , Ferro , Oxirredução
17.
Int Ophthalmol ; 40(5): 1277-1284, 2020 May.
Artigo em Inglês | MEDLINE | ID: mdl-31981000

RESUMO

OBJECTIVE: To investigate the dry eye symptoms after cataract surgery in MGD patients and their relationships METHODS: The study included 115 patients (115 eyes) with age-related cataract that underwent uncomplicated cataract surgery, and the patients were divided into two groups according to the MGD diagnostic criteria: group A (MGD group) and group B (control group). Schirmer I test (ST-I), tear breakup time (TBUT), and corneal fluorescein staining (CFS) were performed preoperatively and at 3 days, 7 days, 14 days, and 30 days postoperatively. We also measured eyelid meibomian gland morphology, meibomian gland expression, and meibum character scores before and after the cataract surgery. RESULTS: Postoperatively, in group A, TBUT decreased and CFS scores increased significantly. ST-I increased in the early postoperative course but decreased later. The eyelid margin morphology scores and meibomian gland expression scores of group A significantly increased after the cataract operation. Thus, patients with MGD may have a greater chance of developing the dry eye disease after cataract surgery. Cataract surgery may aggravate the signs of MGD, and the severity of MGD may positively correlate with TBUT, CFS, and corneal lesions after surgery. CONCLUSIONS: The characteristics of dry eye after cataract surgery in patients with MGD are different from common cataract patients, changes in the early postoperative phase to the ocular surface were caused by surgical factors, and the damages to epithelial function in the later postoperative phase were mainly associated with the inflammation of the meibomian gland and eyelid.


Assuntos
Extração de Catarata/efeitos adversos , Síndromes do Olho Seco/diagnóstico , Glândulas Tarsais/patologia , Complicações Pós-Operatórias , Idoso , Idoso de 80 Anos ou mais , Síndromes do Olho Seco/etiologia , Feminino , Fluorofotometria , Seguimentos , Humanos , Masculino , Pessoa de Meia-Idade , Estudos Prospectivos
18.
Mater Sci Eng C Mater Biol Appl ; 107: 110313, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31761227

RESUMO

Blood brain barrier (BBB), a barrier formed by endothelial cells, separates the brain from the circulatory system and protects the stability of central neural system normally, however, it also results in low permeability of vast majority of drugs for brain disease therapy. In this work, the cytotoxicity, uptake and transportation of 2D graphene nanosheet through BBB were investigated in in vitro models of BBB constructed by human brain microvascular endothelia cells (hBMECs). Permeability of two types of graphene nanosheet, including graphene oxide (GO) and porphyrin conjugated graphene oxide (PGO) through BBB were studied. With hydrophobic chemicals conjugation on its surface, permeability of PGO was greatly improved compared to GO. Furthermore, transportation behavior of assorted sizes of PGO obtained by differential velocity centrifugation through BBB was also explored, revealing that PGO with larger size has higher permeability than smaller-size PGO. The significant improved permeability of 2D graphene nanosheet through BBB compared to traditional drugs provides promising applications in drug delivery and disease therapy for brain disease in the near future.


Assuntos
Barreira Hematoencefálica/metabolismo , Sistemas de Liberação de Medicamentos/métodos , Grafite , Porfirinas , Linhagem Celular , Células Cultivadas , Células Endoteliais/citologia , Células Endoteliais/metabolismo , Grafite/química , Grafite/farmacocinética , Humanos , Nanoestruturas/química , Porfirinas/química , Porfirinas/farmacocinética
19.
Polymers (Basel) ; 11(11)2019 Nov 13.
Artigo em Inglês | MEDLINE | ID: mdl-31766185

RESUMO

In this study, tough and conductive hydrogels were printed by 3D printing method. The combination of thermo-responsive agar and ionic-responsive alginate can highly improve the shape fidelity. With addition of agar, ink viscosity was enhanced, further improving its rheological characteristics for a precise printing. After printing, the printed construct was cured via free radical polymerization, and alginate was crosslinked by calcium ions. Most importantly, with calcium crosslinking of alginate, mechanical properties of 3D printed hydrogels are greatly improved. Furthermore, these 3D printed hydrogels can serve as ionic conductors, because hydrogels contain large amounts of water that dissolve excess calcium ions. A wearable resistive strain sensor that can quickly and precisely detect human motions like finger bending was fabricated by a 3D printed hydrogel film. These results demonstrate that the conductive, transparent, and stretchable hydrogels are promising candidates as soft wearable electronics for healthcare, robotics and entertainment.

20.
Nanomaterials (Basel) ; 9(10)2019 Oct 18.
Artigo em Inglês | MEDLINE | ID: mdl-31635384

RESUMO

Artificial tissue materials usually suffer properties and structure loss over time. As a usual strategy, a new substitution is required to replace the worn one to maintain the functions. Although several approaches have been developed to restore the mechanical properties of hydrogels, they require direct heating or touching, which cannot be processed within the body. In this manuscript, a photothermal method was developed to restore the mechanical properties of the tough hydrogels by using near infrared (NIR) laser irradiation. By adding the porphyrin decorated graphene oxide (PGO) as the nanoreinforcer and photothermal agent into carrageenan/polyacrylamide double network hydrogels (PDN), the compressive strength of the PDN was greatly improved by 104%. Under a short time of NIR laser irradiation, the PGO effectively converts light energy to thermal energy to heat the PDN hydrogels. The damaged carrageenan network was rebuilt, and a 90% compressive strength recovery was achieved. The PGO not only significantly improves the mechanical performance of PDN, but also restores the compressive property of PDN via a photothermal method. These tough hydrogels with superior photothermal recovery may work as promising substitutes for load-bearing tissues.

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